MD14G1143700.v1.1

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011882
Physical Location & Seq
Reverse (-)
23634090 .. 23637027
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1143700.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGACGACGACGTCAACAACAAACCCCAACAACGCCAATCCCAGATCGCCCAAAATTCCGCGTTACCGTCAGAGCCACGTGGCAACCGCCGTCATCGCCGTCGCGGCCCTCTTGATCTCCACCACCGCTTGGCTTTCCCTCGTCTTTTCCGCCACCCCGCACTCCCTCCCCGCCCCACAGTCCTTCACTCGCTCCCGAAACCGCACCATTTCGCGACGGGGTGGACACTCTGAGGAGCTCACTCTAAACGACATCGTTTTCGGAATTGCCGGGTCGGCCCAACTCTGGAAGCAGCGGAAGGAGTACGTCCGGCTCTGGTGGCGCCCGAACGACATGCGCGGCCACGTATGGCTGGAAGAGAAGCTACCGGTAAACGACGTCGATCGGTCGTTGCCGCCGATCATGGTCTCCGAGGACATCTCGAGGTTTCGGTACACGAATCCGACGGGTCACCCGTCGGGGCTTCGAATCTCCCGGATAATCTCCGAGTGCTTCCGCCTTGGCCTCCCCAATGTCCGGTGGTTCGTCCTAGCCGACGACGACACCATCATCAACGTCGATAACCTCGTCGCCGTGCTGGGCAAGTACGACTCGTCGGAGATGGTTTATGTGGGAAGCCCGTCGGAGAGTCACTCTGCCAACACTTATTTCAGCCACTCTATGGCGTTTGGCGGGGGCGGCATTGCAATTAGCCACCCTTTGGCGGAAGCCCTATCCGGAATGCAGGACAAGTGTCTCGAGAGGTACCCGAAGCTCTATGGAAGCGACGATCGTCTCCATGCCTGCATTGCTGAGCTTGGGATTCCATTAACCAGGGAACCTGGCTTCCATCAGTGTGACATTAGGGGCAATGCACACGGTCTTTTATCTTCACATCCTATAGCACCTTTTGTGTCCATTCACCATGTTGAAGCTGTTGACCCATTTTATCCCGGCTTCACCTCTCTGCAAAGTTTGAAGCTTTTCACACAGGCAATGAGACTCCAAGCTACCAGCTTTTTGCAGCGGTCCATTTGTTATGATCGTGGACACCATCTCACATTTTCTGTCTCGCTTGGTTATGTGGTTCAAGTCTTCCCAAACATTGTGCTCCCTCGTGACCTCGAGCGCTCAGAGCAGACTTATTCTGCTTGGAATGGGATAAGTCACAGGAATGAATTCGATTTCGATACAAAAGTCGCTTATAGGTCTGTTTGTAAGAAGCCTATTTTATTCTTCTTGAAAGATATTAGGAAACAGGGGGATGCTACATTGGGATCATATATGCGGAGCAAAGCAAAAGATGATTTCAAGAGAAAGGTTTTCTGCTTTTCCCGATTTCGACTTTTGCACAGTCTGCAAAAAATTAAAGTTTTGGGCTATCCGCCCACCAAGAAATGGCATTTGGTACCGCGGCGGTTATGTTGCAAACTGAACCAAACAAGTGAGGAAGTTGTCAGTTTAACAGTGGGACAGTGTGGTAAAGGACCTTTCGGCTCGATTACTGATACTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

499

Amino Acids

56.08

Weight (kDa)

9.5

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 171 - 278 9.7e-18 Fringe-like
DUF604 PF04646 214 - 469 1.1e-99 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33250
fragaria_vesca FvH4_5g10130
malus_domestica MD06G1127900.v1.1 MD14G1143700.v1.1
prunus_persica Prupe.5G137700_v2.0.a1 Prupe.5G137700_v2.0.a1
pyrus_communis pycom06g11820 pycom14g11910
rosa_chinensis RchiOBHm_Chr7g0188171
rosa_laevigata RLG00000004722
rosa_multiflora Rmu_sc0004001.1_g000001
rosa_roxburghii Rroxscaffold_3G00266710
rosa_rugosa Rorug06G0488800 Rorug06G0488900.1
rosa_samantha Rh7AG093000 Rh7BG094600 Rh7CG094300 Rh7DG095000
rosa_wichuraiana Rw7G008030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 2 cut(s) 14, 381
Acc65I GGTACC 2 cut(s) 744, 1387
AccB1I GGYRCC 3 cut(s) 321, 744, 1387
AccB7I CCANNNNNTGG 4 cut(s) 129, 661, 700, 1377
AccII CGCG 5 cut(s) 61, 104, 214, 339, 1393
AccIII TCCGGA 1 cut(s) 716
AclWI GGATC 1 cut(s) 1264
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 2 cut(s) 54, 1157
AcvI CACGTG 1 cut(s) 79
AcyI GRCGYC 3 cut(s) 11, 322, 378
AfaI GTAC 5 cut(s) 305, 434, 587, 746, 1389
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 7 cut(s) 129, 285, 516, 661, 700, 703, 1377
AgeI ACCGGT 1 cut(s) 367
AgsI TTSAA 5 cut(s) 911, 958, 1070, 1222, 1291
AjnI CCWGG 2 cut(s) 812, 820
AjuI GAANNNNNNNTTGG 4 cut(s) 632, 664, 1367, 1399
AloI GAACNNNNNNTCC 2 cut(s) 810, 842
AluBI AGCT 8 cut(s) 238, 364, 754, 796, 914, 961, 989, 996
AluI AGCT 8 cut(s) 238, 364, 754, 796, 914, 961, 989, 996
Alw21I GWGCWC 2 cut(s) 240, 1092
Alw26I GTCTC 5 cut(s) 412, 740, 779, 973, 1054
AlwI GGATC 1 cut(s) 1264
Ama87I CYCGRG 3 cut(s) 421, 737, 1103
Aor13HI TCCGGA 1 cut(s) 716
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 4 cut(s) 105, 276, 340, 502
ApeKI GCWGC 2 cut(s) 292, 1003
ApoI RAATTY 2 cut(s) 54, 1157
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AsiGI ACCGGT 1 cut(s) 367
Asp700I GAANNNNTTC 2 cut(s) 962, 1157
Asp718I GGTACC 2 cut(s) 744, 1387
AspLEI GCGC 3 cut(s) 324, 339, 1110
AspS9I GGNCC 4 cut(s) 106, 277, 1008, 1466
AsuC2I CCSGG 3 cut(s) 271, 475, 933
AsuHPI GGTGA 3 cut(s) 443, 893, 931
AsuII TTCGAA 1 cut(s) 466
AvaI CYCGRG 3 cut(s) 421, 737, 1103
AvaII GGWCC 2 cut(s) 1008, 1466
BaeI ACNNNNGTAYC 2 cut(s) 1161, 1194
BanI GGYRCC 3 cut(s) 321, 744, 1387
BanII GRGCYC 1 cut(s) 240
BarI GAAGNNNNNNTAC 2 cut(s) 348, 380
BauI CACGAG 1 cut(s) 1095
BbrPI CACGTG 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 1066
Bbv12I GWGCWC 2 cut(s) 240, 1092
BbvI GCAGC 2 cut(s) 304, 1015
BccI CCATC 4 cut(s) 554, 595, 837, 1041
BceAI ACGGC 3 cut(s) 74, 83, 557
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 2 cut(s) 814, 822
BcnI CCSGG 3 cut(s) 271, 475, 933
BcoDI GTCTC 5 cut(s) 412, 740, 779, 973, 1054
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 879
BfoI RGCGCY 2 cut(s) 325, 1111
BglI GCCNNNNNGGC 2 cut(s) 104, 275
BisI GCNGC 7 cut(s) 105, 293, 340, 395, 679, 1004, 1394
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 7 cut(s) 106, 294, 341, 396, 680, 1005, 1395
Bme1390I CCNGG 5 cut(s) 271, 475, 814, 822, 933
Bme18I GGWCC 2 cut(s) 1008, 1466
BmeT110I CYCGRG 3 cut(s) 421, 737, 1103
BmgT120I GGNCC 4 cut(s) 106, 277, 1008, 1466
BmiI GGNNCC 4 cut(s) 323, 746, 819, 1389
BmrFI CCNGG 5 cut(s) 271, 475, 814, 822, 933
BmsI GCATC 1 cut(s) 1234
BoxI GACNNNNGTC 2 cut(s) 732, 771
BpiI GAAGAC 1 cut(s) 1066
BplI GAGNNNNNCTC 4 cut(s) 404, 436, 617, 649
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 466
BpuMI CCSGG 3 cut(s) 271, 475, 933
BsaAI YACGTR 2 cut(s) 79, 346
BsaHI GRCGYC 3 cut(s) 11, 322, 378
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 4 cut(s) 411, 499, 813, 1391
BsaWI WCCGGW 3 cut(s) 367, 516, 716
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 285, 516, 661, 700, 703, 1377
Bse118I RCCGGY 1 cut(s) 367
Bse3DI GCAATG 4 cut(s) 681, 786, 856, 981
BseAI TCCGGA 1 cut(s) 716
BseBI CCWGG 2 cut(s) 814, 822
BseDI CCNNGG 4 cut(s) 411, 499, 813, 1391
BseGI GGATG 2 cut(s) 874, 1249
BseLI CCNNNNNNNGG 7 cut(s) 129, 285, 516, 661, 700, 703, 1377
BseMI GCAATG 4 cut(s) 681, 786, 856, 981
BseMII CTCAG 3 cut(s) 222, 783, 1125
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 2 cut(s) 304, 1015
BseYI CCCAGC 1 cut(s) 577
Bsh1236I CGCG 5 cut(s) 61, 104, 214, 339, 1393
Bsh1285I CGRYCG 3 cut(s) 385, 389, 772
BshFI GGCC 4 cut(s) 107, 278, 342, 504
BshNI GGYRCC 3 cut(s) 321, 744, 1387
BshTI ACCGGT 1 cut(s) 367
BsiEI CGRYCG 3 cut(s) 385, 389, 772
BsiHKAI GWGCWC 2 cut(s) 240, 1092
BsiHKCI CYCGRG 3 cut(s) 421, 737, 1103
BsiSI CCGG 7 cut(s) 270, 310, 368, 475, 517, 717, 933
BslFI GGGAC 1 cut(s) 1464
BslI CCNNNNNNNGG 7 cut(s) 129, 285, 516, 661, 700, 703, 1377
BsmAI GTCTC 5 cut(s) 412, 740, 779, 973, 1054
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 1464
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 4 cut(s) 107, 278, 342, 504
Bso31I GGTCTC 1 cut(s) 412
BsoBI CYCGRG 3 cut(s) 421, 737, 1103
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 240, 1092
Bsp13I TCCGGA 1 cut(s) 716
Bsp143I GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp68I TCGCGA 1 cut(s) 214
BspANI GGCC 4 cut(s) 107, 278, 342, 504
BspCNI CTCAG 3 cut(s) 223, 784, 1124
BspEI TCCGGA 1 cut(s) 716
BspFNI CGCG 5 cut(s) 61, 104, 214, 339, 1393
BspLI GGNNCC 4 cut(s) 323, 746, 819, 1389
BspPI GGATC 1 cut(s) 1264
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 3 cut(s) 321, 744, 1387
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 4 cut(s) 681, 786, 856, 981
BsrFI RCCGGY 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 4 cut(s) 411, 499, 813, 1391
BssMI GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
BssNI GRCGYC 3 cut(s) 11, 322, 378
BssSI CACGAG 1 cut(s) 1095
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 2 cut(s) 814, 822
Bst4CI ACNGT 6 cut(s) 68, 180, 860, 1334, 1447, 1455
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 3 cut(s) 11, 322, 378
BstAPI GCANNNNNTGC 1 cut(s) 1336
BstBAI YACGTR 2 cut(s) 79, 346
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 784
BstDEI CTNAG 3 cut(s) 231, 792, 1111
BstDSI CCRYGG 1 cut(s) 1391
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 2 cut(s) 874, 1249
BstFNI CGCG 5 cut(s) 61, 104, 214, 339, 1393
BstH2I RGCGCY 2 cut(s) 325, 1111
BstHHI GCGC 3 cut(s) 324, 339, 1110
BstKTI GATC 7 cut(s) 47, 117, 385, 402, 772, 1024, 1259
BstMAI GTCTC 5 cut(s) 412, 740, 779, 973, 1054
BstMBI GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
BstMCI CGRYCG 3 cut(s) 385, 389, 772
BstMWI GCNNNNNNNGC 8 cut(s) 95, 104, 275, 319, 678, 788, 1114, 1336
BstNI CCWGG 2 cut(s) 814, 822
BstNSI RCATGY 1 cut(s) 337
BstPAI GACNNNNGTC 2 cut(s) 732, 771
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 5 cut(s) 269, 473, 812, 820, 931
BstSFI CTRYAG 1 cut(s) 879
BstUI CGCG 5 cut(s) 61, 104, 214, 339, 1393
BstV1I GCAGC 2 cut(s) 304, 1015
BstV2I GAAGAC 1 cut(s) 1066
BsuRI GGCC 4 cut(s) 107, 278, 342, 504
BtgI CCRYGG 1 cut(s) 1391
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 874, 1249
BtsIMutI CAGTG 3 cut(s) 839, 1452, 1460
BtuMI TCGCGA 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 784
CfoI GCGC 3 cut(s) 324, 339, 1110
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 4 cut(s) 106, 277, 1008, 1466
Cfr42I CCGCGG 1 cut(s) 1394
Csp6I GTAC 5 cut(s) 304, 433, 586, 745, 1388
CspAI ACCGGT 1 cut(s) 367
CviAII CATG 4 cut(s) 334, 403, 779, 905
CviQI GTAC 5 cut(s) 304, 433, 586, 745, 1388
DdeI CTNAG 3 cut(s) 231, 792, 1111
DinI GGCGCC 1 cut(s) 323
DpnI GATC 7 cut(s) 46, 116, 384, 401, 771, 1023, 1258
DpnII GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 4 cut(s) 139, 485, 719, 1353
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 1008, 1466
Eco47III AGCGCT 1 cut(s) 1109
Eco53kI GAGCTC 1 cut(s) 238
Eco72I CACGTG 1 cut(s) 79
Eco88I CYCGRG 3 cut(s) 421, 737, 1103
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 1466
EcoO65I GGTNACC 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 1157
EcoRII CCWGG 2 cut(s) 812, 820
EcoT14I CCWWGG 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 323
EheI GGCGCC 1 cut(s) 323
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 4 cut(s) 337, 406, 782, 908
FaqI GGGAC 1 cut(s) 1464
FatI CATG 4 cut(s) 333, 402, 778, 904
FauI CCCGC 3 cut(s) 165, 178, 665
Fnu4HI GCNGC 7 cut(s) 105, 293, 340, 395, 679, 1004, 1394
FokI GGATG 2 cut(s) 861, 1256
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 7 cut(s) 105, 293, 340, 395, 679, 1004, 1394
FspBI CTAG 1 cut(s) 530
GlaI GCGC 3 cut(s) 323, 338, 1109
GluI GCNGC 7 cut(s) 105, 293, 340, 395, 679, 1004, 1394
GsaI CCCAGC 1 cut(s) 581
HaeII RGCGCY 2 cut(s) 325, 1111
HaeIII GGCC 4 cut(s) 107, 278, 342, 504
HapII CCGG 7 cut(s) 270, 310, 368, 475, 517, 717, 933
HhaI GCGC 3 cut(s) 324, 339, 1110
Hin1I GRCGYC 3 cut(s) 11, 322, 378
Hin1II CATG 4 cut(s) 337, 406, 782, 908
Hin6I GCGC 3 cut(s) 322, 337, 1108
HinP1I GCGC 3 cut(s) 322, 337, 1108
HincII GTYRAC 2 cut(s) 15, 919
HindII GTYRAC 2 cut(s) 15, 919
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 6 cut(s) 439, 468, 590, 628, 802, 981
HpaII CCGG 7 cut(s) 270, 310, 368, 475, 517, 717, 933
HphI GGTGA 3 cut(s) 443, 893, 931
Hpy166II GTNNAC 6 cut(s) 15, 224, 373, 435, 919, 1028
Hpy188I TCNGA 9 cut(s) 72, 232, 263, 412, 444, 487, 598, 625, 1114
Hpy8I GTNNAC 6 cut(s) 15, 224, 373, 435, 919, 1028
HpyAV CCTTC 2 cut(s) 193, 292
HpyCH4III ACNGT 6 cut(s) 68, 180, 860, 1334, 1447, 1455
HpyCH4IV ACGT 6 cut(s) 11, 78, 306, 345, 378, 555
HpyCH4V TGCA 9 cut(s) 686, 724, 786, 854, 949, 1003, 1330, 1339, 1407
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 104, 275, 319, 678, 788, 1114, 1336
HpyF3I CTNAG 3 cut(s) 231, 792, 1111
HpySE526I ACGT 6 cut(s) 11, 78, 306, 345, 378, 555
Hsp92I GRCGYC 3 cut(s) 11, 322, 378
Hsp92II CATG 4 cut(s) 337, 406, 782, 908
HspAI GCGC 3 cut(s) 322, 337, 1108
KasI GGCGCC 1 cut(s) 321
Kpn2I TCCGGA 1 cut(s) 716
KpnI GGTACC 2 cut(s) 748, 1391
KspI CCGCGG 1 cut(s) 1394
Kzo9I GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
LmnI GCTCC 4 cut(s) 197, 235, 1095, 1269
Lsp1109I GCAGC 2 cut(s) 304, 1015
LweI GCATC 1 cut(s) 1234
MaeI CTAG 1 cut(s) 530
MaeII ACGT 6 cut(s) 11, 78, 306, 345, 378, 555
MaeIII GTNAC 6 cut(s) 62, 449, 629, 836, 1097, 1145
MalI GATC 7 cut(s) 46, 116, 384, 401, 771, 1023, 1258
MboI GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
MboII GAAGA 4 cut(s) 368, 861, 1066, 1207
MhlI GDGCHC 2 cut(s) 240, 1092
MluCI AATT 5 cut(s) 54, 264, 687, 1157, 1344
Mly113I GGCGCC 1 cut(s) 322
MlyI GAGTC 3 cut(s) 584, 637, 975
MmeI TCCRAC 3 cut(s) 467, 576, 603
MroI TCCGGA 1 cut(s) 716
MroXI GAANNNNTTC 2 cut(s) 962, 1157
MseI TTAA 3 cut(s) 809, 1347, 1442
MslI CAYNNNNRTG 2 cut(s) 834, 1152
MspA1I CMGCKG 3 cut(s) 295, 1006, 1393
MspI CCGG 7 cut(s) 270, 310, 368, 475, 517, 717, 933
MspR9I CCNGG 5 cut(s) 271, 475, 814, 822, 933
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 2 cut(s) 814, 822
MvnI CGCG 5 cut(s) 61, 104, 214, 339, 1393
MwoI GCNNNNNNNGC 8 cut(s) 95, 104, 275, 319, 678, 788, 1114, 1336
NarI GGCGCC 1 cut(s) 322
NciI CCSGG 3 cut(s) 271, 475, 933
NdeII GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
NlaIII CATG 4 cut(s) 337, 406, 782, 908
NlaIV GGNNCC 4 cut(s) 323, 746, 819, 1389
NmuCI GTSAC 5 cut(s) 449, 629, 836, 1097, 1145
NruI TCGCGA 1 cut(s) 214
NspI RCATGY 1 cut(s) 337
NspV TTCGAA 1 cut(s) 466
PaeR7I CTCGAG 3 cut(s) 421, 737, 1103
PcsI WCGNNNNNNNCGW 4 cut(s) 395, 452, 531, 564
PctI GAATGC 1 cut(s) 726
PdmI GAANNNNTTC 2 cut(s) 962, 1157
PfeI GAWTC 3 cut(s) 439, 468, 802
PflFI GACNNNGTC 1 cut(s) 10
PflMI CCANNNNNTGG 4 cut(s) 129, 661, 700, 1377
PfoI TCCNGGA 1 cut(s) 473
PinAI ACCGGT 1 cut(s) 367
PkrI GCNGC 7 cut(s) 106, 294, 341, 396, 680, 1005, 1395
Ple19I CGATCG 2 cut(s) 385, 772
PleI GAGTC 3 cut(s) 584, 636, 975
PluTI GGCGCC 1 cut(s) 325
PmaCI CACGTG 1 cut(s) 79
PmlI CACGTG 1 cut(s) 79
PpsI GAGTC 3 cut(s) 584, 636, 975
Ppu21I YACGTR 2 cut(s) 79, 346
PpuMI RGGWCCY 1 cut(s) 1466
PshAI GACNNNNGTC 2 cut(s) 732, 771
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 1466
Psp6I CCWGG 2 cut(s) 812, 820
PspCI CACGTG 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 449
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 2 cut(s) 812, 820
PspN4I GGNNCC 4 cut(s) 323, 746, 819, 1389
PspPI GGNCC 4 cut(s) 106, 277, 1008, 1466
PspPPI RGGWCCY 1 cut(s) 1466
PspXI VCTCGAGB 1 cut(s) 1103
PsyI GACNNNGTC 1 cut(s) 10
PvuI CGATCG 2 cut(s) 385, 772
RruI TCGCGA 1 cut(s) 214
RsaI GTAC 5 cut(s) 305, 434, 587, 746, 1389
RsaNI GTAC 5 cut(s) 304, 433, 586, 745, 1388
RseI CAYNNNNRTG 2 cut(s) 834, 1152
SacI GAGCTC 1 cut(s) 240
SacII CCGCGG 1 cut(s) 1394
SaqAI TTAA 3 cut(s) 809, 1347, 1442
SatI GCNGC 7 cut(s) 105, 293, 340, 395, 679, 1004, 1394
Sau3AI GATC 7 cut(s) 44, 114, 382, 399, 769, 1021, 1256
Sau96I GGNCC 4 cut(s) 106, 277, 1008, 1466
SchI GAGTC 3 cut(s) 584, 637, 975
ScrFI CCNGG 5 cut(s) 271, 475, 814, 822, 933
SduI GDGCHC 2 cut(s) 240, 1092
SfaNI GCATC 1 cut(s) 1234
SfcI CTRYAG 1 cut(s) 879
SfoI GGCGCC 1 cut(s) 323
Sfr274I CTCGAG 3 cut(s) 421, 737, 1103
Sfr303I CCGCGG 1 cut(s) 1394
SfuI TTCGAA 1 cut(s) 466
SgrBI CCGCGG 1 cut(s) 1394
SinI GGWCC 2 cut(s) 1008, 1466
SlaI CTCGAG 3 cut(s) 421, 737, 1103
SmiMI CAYNNNNRTG 2 cut(s) 834, 1152
SmlI CTYRAG 3 cut(s) 421, 737, 1103
SmoI CTYRAG 3 cut(s) 421, 737, 1103
Sse9I AATT 5 cut(s) 54, 264, 687, 1157, 1344
SspDI GGCGCC 1 cut(s) 321
SspMI CTAG 1 cut(s) 530
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 5 cut(s) 269, 473, 812, 820, 931
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 6 cut(s) 68, 180, 860, 1334, 1447, 1455
TaiI ACGT 6 cut(s) 14, 81, 309, 348, 381, 558
TaqII GACCGA 1 cut(s) 375
TasI AATT 5 cut(s) 54, 264, 687, 1157, 1344
TauI GCSGC 5 cut(s) 107, 342, 397, 681, 1396
TfiI GAWTC 3 cut(s) 439, 468, 802
Tru1I TTAA 3 cut(s) 809, 1347, 1442
Tru9I TTAA 3 cut(s) 809, 1347, 1442
TscAI CASTG 3 cut(s) 839, 1452, 1460
TseFI GTSAC 5 cut(s) 449, 629, 836, 1097, 1145
TseI GCWGC 2 cut(s) 292, 1003
Tsp45I GTSAC 5 cut(s) 449, 629, 836, 1097, 1145
TspDTI ATGAA 1 cut(s) 1170
TspRI CASTG 3 cut(s) 839, 1452, 1460
Tth111I GACNNNGTC 1 cut(s) 10
Van91I CCANNNNNTGG 4 cut(s) 129, 661, 700, 1377
VpaK11BI GGWCC 2 cut(s) 1008, 1466
XapI RAATTY 2 cut(s) 54, 1157
XceI RCATGY 1 cut(s) 337
XhoI CTCGAG 3 cut(s) 421, 737, 1103
XmnI GAANNNNTTC 2 cut(s) 962, 1157
XspI CTAG 1 cut(s) 530
ZraI GACGTC 2 cut(s) 12, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.