FvH4_5g10130

Fringe-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011882
Physical Location & Seq
Forward (+)
5773551 .. 5777209
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10130.t1

Sequence Viewer

Length: 1575 bp
ATGACGACCATGTCAACCAACCCCAACAACAATCCCAATCCCAATCCCAATCCCAATCCCAAAGACCAAACCAATCTCCGATCACCCAAGAGCCGTCGATGGCGGCAGAGCCAGGTGGCGACTGCCGTGATCGCCATCGCGGCTCTCTTGATCTCCACCGCCGCGTGGCTCTCTCTTGTCTTCTCCGGCAGCCCGACTCTTCCGGTCGCCCGCTTGCAAAACAAGGCCCCGGCTCCGGCTTTCACGCAGTACCAAAACGACAGCGTTTCGGTTTTCGATGACGAGGATGACCCGGTGGAGTGGGGCGACGAGGAGCTGTCGCTAGACGACGTCGTTTTCGGGATTGCTGGGTCGTCCGGGCTCTGGAAGCAGCGGAAGGAGTACGTCAGGCTGTGGTGGCGTCCGGACGTCATGCGCGGACACGTCTGGATGGAAGAGAAAGTTCCGGAAGAACAAAACGACGAGTCGTTGCCGCCGATCATGGTCTCCGAGGACATCTCGCGGTTCCGGTACACCAACCCGACGGGTCACCCTTCGGGGCTCCGAATCTCGCGGATAATCTCCGAGTGCTTCCGCCTCCGCCTGCCCAAAGTGCGGTGGTTCGTGCTGGCGGACGACGACACCATCATCAATGTCGTTAACCTCGTGGCGGTTCTGAGCAAATACGACGCGTCGGAGATGGTTTATGTGGGGAGTCCGTCGGAGAGTCACTCTGCGAATACGTATTTCAGTCATTCCATGGCTTTCGGCGGTGGAGGTATTGCGATTAGCTATCCTCTGGCGAAAGCTCTCTCCGAAATTCAAGACGAGTGTCTCGAGAGGTATCCGAAACTATACGGGAGTGATGATCGACTCCATGCCTGCATTACTGAGCTCGGGGTTCCTTTAACTCGAGAGCCTGGCTTCCACCAGTGTGATATTAGAGGCAATGCGCATGGTCTTCTATCTTCTCATCCGATAGCACCATTTGTGTCGATTCACCATGTTGAAGCTGTGGATCCTATTTATCCTGGCTTAAACTCTTTAGAGAGTTTGAAGCTCTTTACACAGGCTATGAGACTCCAACCCAGCAGTTTTTTGCAGCGTTCCATTTGTTATGATCGCCGACACCATCTCACATTTTCGGTTTCTCTTGGTTATGTGGTTCAAGTCTTCCCGCAAATTGTGCTTCCTCGGGACCTTGAGCGATCAGAGCAGACTTACTCGGCTTGGAATGGAATCAGTAAAAGGAGTGAATTTGATTTGGACACAAAAGACGCGTACAGGTCTGTTTGTAAGAAACCTATTCTGTTATTCTTGAAAGATATCAGGTCGCAGGGTAATGCTACTCTGGGATCTTATATGAGGAGTAGAGCAAAAGATGATTTCAAAAGAACAGTTCTATGCTTTCCCCGGTCCCGGCATTTGCGCCATCTACGAGAAATTCAAGTTTTGGGCCATCCACTCACCAAGAAATGGCATTTGGTACCGCGCCGGCTATGTTGCAAACTGAACCAAAACAGTGATGAGGTCCTTAGATTAACAGTTGGACAATGTGCTAAGGGAGCTTCTGGTTCTTTTACTGATTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

525

Amino Acids

59.25

Weight (kDa)

8.56

Isoelectric Point (pI)

54.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fringe PF02434 197 - 304 4.1e-18 Fringe-like
DUF604 PF04646 240 - 495 3.9e-101 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33250
fragaria_vesca FvH4_5g10130
malus_domestica MD06G1127900.v1.1 MD14G1143700.v1.1
prunus_persica Prupe.5G137700_v2.0.a1 Prupe.5G137700_v2.0.a1
pyrus_communis pycom06g11820 pycom14g11910
rosa_chinensis RchiOBHm_Chr7g0188171
rosa_laevigata RLG00000004722
rosa_multiflora Rmu_sc0004001.1_g000001
rosa_roxburghii Rroxscaffold_3G00266710
rosa_rugosa Rorug06G0488800 Rorug06G0488900.1
rosa_samantha Rh7AG093000 Rh7BG094600 Rh7CG094300 Rh7DG095000
rosa_wichuraiana Rw7G008030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1569
AasI GACNNNNNNGTC 1 cut(s) 10
AatII GACGTC 2 cut(s) 333, 411
Acc16I TGCGCA 1 cut(s) 933
Acc65I GGTACC 1 cut(s) 1465
AccB1I GGYRCC 1 cut(s) 1465
AccB7I CCANNNNNTGG 1 cut(s) 1455
AccII CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
AccIII TCCGGA 2 cut(s) 403, 445
AclWI GGATC 3 cut(s) 992, 1005, 1342
AcsI RAATTY 3 cut(s) 798, 1235, 1422
AcyI GRCGYC 3 cut(s) 330, 400, 408
AfaI GTAC 5 cut(s) 251, 383, 512, 1262, 1467
AfiI CCNNNNNNNGG 7 cut(s) 165, 235, 363, 594, 649, 1398, 1455
AflIII ACRYGT 3 cut(s) 421, 669, 1257
AgsI TTSAA 7 cut(s) 803, 989, 1036, 1148, 1300, 1369, 1427
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 3 cut(s) 111, 898, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 1445, 1477
AleI CACNNNNGTG 1 cut(s) 912
AluBI AGCT 7 cut(s) 316, 771, 788, 874, 992, 1039, 1547
AluI AGCT 7 cut(s) 316, 771, 788, 874, 992, 1039, 1547
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 3 cut(s) 490, 818, 1051
AlwI GGATC 3 cut(s) 992, 1005, 1342
Ama87I CYCGRG 4 cut(s) 815, 875, 891, 1173
Aor13HI TCCGGA 2 cut(s) 403, 445
AoxI GGCC 2 cut(s) 225, 1435
ApeKI GCWGC 3 cut(s) 189, 370, 1081
ApoI RAATTY 3 cut(s) 798, 1235, 1422
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp718I GGTACC 1 cut(s) 1465
AspLEI GCGC 4 cut(s) 417, 934, 1410, 1473
AspS9I GGNCC 5 cut(s) 226, 1177, 1395, 1435, 1510
AsuC2I CCSGG 5 cut(s) 230, 293, 358, 1393, 1399
AsuHPI GGTGA 4 cut(s) 75, 521, 971, 1438
AvaI CYCGRG 4 cut(s) 815, 875, 891, 1173
AvaII GGWCC 3 cut(s) 1177, 1395, 1510
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 1465
BanII GRGCYC 3 cut(s) 363, 543, 876
BauI CACGAG 1 cut(s) 644
BbsI GAAGAC 3 cut(s) 172, 932, 1144
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 3 cut(s) 201, 382, 1093
BccI CCATC 8 cut(s) 93, 143, 424, 632, 673, 1119, 1419, 1446
BceAI ACGGC 2 cut(s) 78, 110
BciT130I CCWGG 3 cut(s) 113, 900, 1011
BciVI GTATCC 1 cut(s) 834
BcnI CCSGG 5 cut(s) 230, 293, 358, 1393, 1399
BcoDI GTCTC 3 cut(s) 490, 818, 1051
BfaI CTAG 1 cut(s) 323
BfuI GTATCC 1 cut(s) 834
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 7 cut(s) 104, 141, 162, 190, 371, 473, 1082
BlsI GCNGC 7 cut(s) 105, 142, 163, 191, 372, 474, 1083
Bme1390I CCNGG 8 cut(s) 113, 230, 293, 358, 900, 1011, 1393, 1399
Bme18I GGWCC 3 cut(s) 1177, 1395, 1510
BmeT110I CYCGRG 4 cut(s) 815, 875, 891, 1173
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 5 cut(s) 226, 1177, 1395, 1435, 1510
BmiI GGNNCC 9 cut(s) 228, 234, 506, 542, 882, 999, 1178, 1397, 1467
BmrFI CCNGG 8 cut(s) 113, 230, 293, 358, 900, 1011, 1393, 1399
BoxI GACNNNNGTC 1 cut(s) 810
BpiI GAAGAC 3 cut(s) 172, 932, 1144
BplI GAGNNNNNCTC 4 cut(s) 482, 514, 695, 727
Bpu10I CCTNAGC 1 cut(s) 1539
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 5 cut(s) 230, 293, 358, 1393, 1399
BsaAI YACGTR 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 3 cut(s) 330, 400, 408
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 5 cut(s) 228, 489, 738, 1172, 1391
BsaWI WCCGGW 4 cut(s) 202, 403, 445, 507
Bsc4I CCNNNNNNNGG 7 cut(s) 165, 235, 363, 594, 649, 1398, 1455
Bse118I RCCGGY 1 cut(s) 1473
Bse1I ACTGG 1 cut(s) 910
Bse3DI GCAATG 1 cut(s) 934
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 2 cut(s) 403, 445
BseBI CCWGG 3 cut(s) 113, 900, 1011
BseDI CCNNGG 5 cut(s) 228, 489, 738, 1172, 1391
BseGI GGATG 4 cut(s) 292, 435, 952, 1438
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 7 cut(s) 165, 235, 363, 594, 649, 1398, 1455
BseMI GCAATG 1 cut(s) 934
BseMII CTCAG 2 cut(s) 647, 861
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 2 cut(s) 326, 1360
BseXI GCAGC 3 cut(s) 201, 382, 1093
BseYI CCCAGC 2 cut(s) 347, 1067
Bsh1236I CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 227, 1437
BshNI GGYRCC 1 cut(s) 1465
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 876
BsiHKCI CYCGRG 4 cut(s) 815, 875, 891, 1173
BslFI GGGAC 2 cut(s) 1190, 1381
BslI CCNNNNNNNGG 7 cut(s) 165, 235, 363, 594, 649, 1398, 1455
BsmAI GTCTC 3 cut(s) 490, 818, 1051
BsmFI GGGAC 2 cut(s) 1190, 1381
BsnI GGCC 2 cut(s) 227, 1437
Bso31I GGTCTC 1 cut(s) 490
BsoBI CYCGRG 4 cut(s) 815, 875, 891, 1173
Bsp1286I GDGCHC 3 cut(s) 363, 543, 876
Bsp13I TCCGGA 2 cut(s) 403, 445
Bsp143I GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
Bsp19I CCATGG 1 cut(s) 738
BspANI GGCC 2 cut(s) 227, 1437
BspCNI CTCAG 2 cut(s) 648, 862
BspEI TCCGGA 2 cut(s) 403, 445
BspFNI CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
BspLI GGNNCC 9 cut(s) 228, 234, 506, 542, 882, 999, 1178, 1397, 1467
BspPI GGATC 3 cut(s) 992, 1005, 1342
BspT107I GGYRCC 1 cut(s) 1465
BspTNI GGTCTC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 934
BsrFI RCCGGY 1 cut(s) 1473
BsrI ACTGG 1 cut(s) 910
BssAI RCCGGY 1 cut(s) 1473
BssECI CCNNGG 5 cut(s) 228, 489, 738, 1172, 1391
BssMI GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
BssNI GRCGYC 3 cut(s) 330, 400, 408
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 738
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 3 cut(s) 113, 900, 1011
Bst4CI ACNGT 3 cut(s) 1378, 1502, 1525
Bst6I CTCTTC 2 cut(s) 204, 429
BstACI GRCGYC 3 cut(s) 330, 400, 408
BstAPI GCANNNNNTGC 1 cut(s) 1165
BstBAI YACGTR 1 cut(s) 723
BstC8I GCNNGC 6 cut(s) 211, 215, 584, 609, 862, 1475
BstDEI CTNAG 4 cut(s) 656, 870, 1514, 1539
BstDSI CCRYGG 1 cut(s) 738
BstEII GGTNACC 1 cut(s) 527
BstF5I GGATG 4 cut(s) 292, 435, 952, 1438
BstFNI CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
BstHHI GCGC 4 cut(s) 417, 934, 1410, 1473
BstKTI GATC 9 cut(s) 83, 132, 153, 480, 850, 1000, 1102, 1190, 1337
BstMAI GTCTC 3 cut(s) 490, 818, 1051
BstMBI GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 8 cut(s) 131, 140, 367, 397, 592, 1165, 1192, 1544
BstNI CCWGG 3 cut(s) 113, 900, 1011
BstPAI GACNNNNGTC 1 cut(s) 810
BstPI GGTNACC 1 cut(s) 527
BstSCI CCNGG 8 cut(s) 111, 228, 291, 356, 898, 1009, 1391, 1397
BstSNI TACGTA 1 cut(s) 723
BstUI CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
BstV1I GCAGC 3 cut(s) 201, 382, 1093
BstV2I GAAGAC 3 cut(s) 172, 932, 1144
BstX2I RGATCY 2 cut(s) 997, 1334
BstYI RGATCY 2 cut(s) 997, 1334
BsuI GTATCC 1 cut(s) 834
BsuRI GGCC 2 cut(s) 227, 1437
BtgI CCRYGG 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 121
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 4 cut(s) 292, 435, 952, 1438
BtsIMutI CAGTG 2 cut(s) 917, 1507
Cac8I GCNNGC 6 cut(s) 211, 215, 584, 609, 862, 1475
CfoI GCGC 4 cut(s) 417, 934, 1410, 1473
Cfr10I RCCGGY 1 cut(s) 1473
Cfr13I GGNCC 5 cut(s) 226, 1177, 1395, 1435, 1510
CseI GACGC 4 cut(s) 389, 660, 677, 1265
Csp6I GTAC 5 cut(s) 250, 382, 511, 1261, 1466
CviAII CATG 7 cut(s) 10, 412, 481, 739, 857, 935, 983
CviQI GTAC 5 cut(s) 250, 382, 511, 1261, 1466
DdeI CTNAG 4 cut(s) 656, 870, 1514, 1539
DpnI GATC 9 cut(s) 82, 131, 152, 479, 849, 999, 1101, 1189, 1336
DpnII GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 204, 429
EarI CTCTTC 2 cut(s) 204, 429
EciI GGCGGA 3 cut(s) 563, 569, 626
Ecl136II GAGCTC 1 cut(s) 874
Eco105I TACGTA 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 738
Eco24I GRGCYC 3 cut(s) 363, 543, 876
Eco31I GGTCTC 1 cut(s) 490
Eco32I GATATC 1 cut(s) 1306
Eco47I GGWCC 3 cut(s) 1177, 1395, 1510
Eco53kI GAGCTC 1 cut(s) 874
Eco88I CYCGRG 4 cut(s) 815, 875, 891, 1173
Eco91I GGTNACC 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 874
EcoO109I RGGNCCY 3 cut(s) 226, 1177, 1510
EcoO65I GGTNACC 1 cut(s) 527
EcoRII CCWGG 3 cut(s) 111, 898, 1009
EcoRV GATATC 1 cut(s) 1306
EcoT14I CCWWGG 1 cut(s) 738
EcoT38I GRGCYC 3 cut(s) 363, 543, 876
ErhI CCWWGG 1 cut(s) 738
FaeI CATG 7 cut(s) 13, 415, 484, 742, 860, 938, 986
FaqI GGGAC 2 cut(s) 1190, 1381
FatI CATG 7 cut(s) 9, 411, 480, 738, 856, 934, 982
FauI CCCGC 2 cut(s) 218, 1164
Fnu4HI GCNGC 7 cut(s) 104, 141, 162, 190, 371, 473, 1082
FokI GGATG 4 cut(s) 299, 442, 939, 1425
FriOI GRGCYC 3 cut(s) 363, 543, 876
Fsp4HI GCNGC 7 cut(s) 104, 141, 162, 190, 371, 473, 1082
FspAI RTGCGCAY 1 cut(s) 933
FspBI CTAG 1 cut(s) 323
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 4 cut(s) 416, 933, 1409, 1472
GluI GCNGC 7 cut(s) 104, 141, 162, 190, 371, 473, 1082
GsaI CCCAGC 2 cut(s) 351, 1071
HaeIII GGCC 2 cut(s) 227, 1437
HgaI GACGC 4 cut(s) 389, 660, 677, 1265
HhaI GCGC 4 cut(s) 417, 934, 1410, 1473
Hin1I GRCGYC 3 cut(s) 330, 400, 408
Hin1II CATG 7 cut(s) 13, 415, 484, 742, 860, 938, 986
Hin6I GCGC 4 cut(s) 415, 932, 1408, 1471
HinP1I GCGC 4 cut(s) 415, 932, 1408, 1471
HincII GTYRAC 2 cut(s) 15, 640
HindII GTYRAC 2 cut(s) 15, 640
HinfI GANTC 9 cut(s) 196, 464, 546, 694, 706, 852, 976, 1059, 1218
HpaI GTTAAC 1 cut(s) 640
HphI GGTGA 4 cut(s) 75, 521, 971, 1438
Hpy166II GTNNAC 3 cut(s) 15, 513, 640
Hpy8I GTNNAC 3 cut(s) 15, 513, 640
HpyAV CCTTC 2 cut(s) 370, 543
HpyCH4III ACNGT 3 cut(s) 1378, 1502, 1525
HpyCH4IV ACGT 5 cut(s) 330, 384, 408, 423, 722
HpyCH4V TGCA 4 cut(s) 217, 864, 1081, 1485
HpyF10VI GCNNNNNNNGC 8 cut(s) 131, 140, 367, 397, 592, 1165, 1192, 1544
HpyF3I CTNAG 4 cut(s) 656, 870, 1514, 1539
HpySE526I ACGT 5 cut(s) 330, 384, 408, 423, 722
Hsp92I GRCGYC 3 cut(s) 330, 400, 408
Hsp92II CATG 7 cut(s) 13, 415, 484, 742, 860, 938, 986
HspAI GCGC 4 cut(s) 415, 932, 1408, 1471
Kpn2I TCCGGA 2 cut(s) 403, 445
KpnI GGTACC 1 cut(s) 1469
KroI GCCGGC 1 cut(s) 1473
KroNI GCCGGC 1 cut(s) 1475
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
LmnI GCTCC 4 cut(s) 238, 313, 546, 1544
Lsp1109I GCAGC 3 cut(s) 201, 382, 1093
MaeI CTAG 1 cut(s) 323
MaeII ACGT 5 cut(s) 330, 384, 408, 423, 722
MaeIII GTNAC 2 cut(s) 527, 707
MalI GATC 9 cut(s) 82, 131, 152, 479, 849, 999, 1101, 1189, 1336
MboI GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
MboII GAAGA 7 cut(s) 172, 191, 446, 461, 932, 939, 1144
MflI RGATCY 2 cut(s) 997, 1334
MhlI GDGCHC 3 cut(s) 363, 543, 876
MluCI AATT 4 cut(s) 798, 1161, 1235, 1422
MluI ACGCGT 2 cut(s) 669, 1257
MlyI GAGTC 6 cut(s) 190, 473, 703, 715, 846, 1053
MmeI TCCRAC 4 cut(s) 654, 681, 1087, 1507
MroI TCCGGA 2 cut(s) 403, 445
MroNI GCCGGC 1 cut(s) 1473
MseI TTAA 4 cut(s) 639, 887, 1016, 1520
MslI CAYNNNNRTG 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 373
MspR9I CCNGG 8 cut(s) 113, 230, 293, 358, 900, 1011, 1393, 1399
MvaI CCWGG 3 cut(s) 113, 900, 1011
MvnI CGCG 8 cut(s) 140, 164, 417, 502, 553, 671, 1259, 1471
MwoI GCNNNNNNNGC 8 cut(s) 131, 140, 367, 397, 592, 1165, 1192, 1544
NaeI GCCGGC 1 cut(s) 1475
NciI CCSGG 5 cut(s) 230, 293, 358, 1393, 1399
NcoI CCATGG 1 cut(s) 738
NdeII GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
NgoMIV GCCGGC 1 cut(s) 1473
NlaIII CATG 7 cut(s) 13, 415, 484, 742, 860, 938, 986
NlaIV GGNNCC 9 cut(s) 228, 234, 506, 542, 882, 999, 1178, 1397, 1467
NmeAIII GCCGAG 1 cut(s) 1184
NmuCI GTSAC 2 cut(s) 527, 707
NsbI TGCGCA 1 cut(s) 933
OliI CACNNNNGTG 1 cut(s) 912
PaeR7I CTCGAG 2 cut(s) 815, 891
PcsI WCGNNNNNNNCGW 3 cut(s) 473, 642, 813
PdiI GCCGGC 1 cut(s) 1475
PfeI GAWTC 3 cut(s) 546, 976, 1218
PflFI GACNNNGTC 2 cut(s) 10, 329
PflMI CCANNNNNTGG 1 cut(s) 1455
PkrI GCNGC 7 cut(s) 105, 142, 163, 191, 372, 474, 1083
PleI GAGTC 6 cut(s) 190, 472, 702, 714, 846, 1053
PpsI GAGTC 6 cut(s) 190, 472, 702, 714, 846, 1053
Ppu21I YACGTR 1 cut(s) 723
PpuMI RGGWCCY 2 cut(s) 1177, 1510
PshAI GACNNNNGTC 1 cut(s) 810
PsiI TTATAA 1 cut(s) 1569
Psp124BI GAGCTC 1 cut(s) 876
Psp5II RGGWCCY 2 cut(s) 1177, 1510
Psp6I CCWGG 3 cut(s) 111, 898, 1009
PspEI GGTNACC 1 cut(s) 527
PspFI CCCAGC 2 cut(s) 347, 1067
PspGI CCWGG 3 cut(s) 111, 898, 1009
PspN4I GGNNCC 9 cut(s) 228, 234, 506, 542, 882, 999, 1178, 1397, 1467
PspPI GGNCC 5 cut(s) 226, 1177, 1395, 1435, 1510
PspPPI RGGWCCY 2 cut(s) 1177, 1510
PsuI RGATCY 2 cut(s) 997, 1334
PsyI GACNNNGTC 2 cut(s) 10, 329
RsaI GTAC 5 cut(s) 251, 383, 512, 1262, 1467
RsaNI GTAC 5 cut(s) 250, 382, 511, 1261, 1466
RseI CAYNNNNRTG 1 cut(s) 912
SacI GAGCTC 1 cut(s) 876
SaqAI TTAA 4 cut(s) 639, 887, 1016, 1520
SatI GCNGC 7 cut(s) 104, 141, 162, 190, 371, 473, 1082
Sau3AI GATC 9 cut(s) 80, 129, 150, 477, 847, 997, 1099, 1187, 1334
Sau96I GGNCC 5 cut(s) 226, 1177, 1395, 1435, 1510
SchI GAGTC 6 cut(s) 190, 473, 703, 715, 846, 1053
ScrFI CCNGG 8 cut(s) 113, 230, 293, 358, 900, 1011, 1393, 1399
SduI GDGCHC 3 cut(s) 363, 543, 876
Sfr274I CTCGAG 2 cut(s) 815, 891
SinI GGWCC 3 cut(s) 1177, 1395, 1510
SlaI CTCGAG 2 cut(s) 815, 891
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 3 cut(s) 815, 891, 1181
SmoI CTYRAG 3 cut(s) 815, 891, 1181
SnaBI TACGTA 1 cut(s) 723
Sse9I AATT 4 cut(s) 798, 1161, 1235, 1422
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 8 cut(s) 111, 228, 291, 356, 898, 1009, 1391, 1397
StyI CCWWGG 1 cut(s) 738
TaaI ACNGT 3 cut(s) 1378, 1502, 1525
TaiI ACGT 5 cut(s) 333, 387, 411, 426, 725
TaqI TCGA 6 cut(s) 97, 276, 816, 850, 892, 974
TasI AATT 4 cut(s) 798, 1161, 1235, 1422
TauI GCSGC 4 cut(s) 106, 143, 164, 475
TfiI GAWTC 3 cut(s) 546, 976, 1218
Tru1I TTAA 4 cut(s) 639, 887, 1016, 1520
Tru9I TTAA 4 cut(s) 639, 887, 1016, 1520
TscAI CASTG 2 cut(s) 917, 1507
TseFI GTSAC 2 cut(s) 527, 707
TseI GCWGC 3 cut(s) 189, 370, 1081
Tsp45I GTSAC 2 cut(s) 527, 707
TspGWI ACGGA 1 cut(s) 687
TspRI CASTG 2 cut(s) 917, 1507
Tth111I GACNNNGTC 2 cut(s) 10, 329
Van91I CCANNNNNTGG 1 cut(s) 1455
VpaK11BI GGWCC 3 cut(s) 1177, 1395, 1510
XapI RAATTY 3 cut(s) 798, 1235, 1422
XhoI CTCGAG 2 cut(s) 815, 891
XspI CTAG 1 cut(s) 323
ZraI GACGTC 2 cut(s) 331, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.