AT1G52570

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005265
Physical Location & Seq
Reverse (-)
19583617 .. 19587531
3915 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G52570.1

Sequence Viewer

Length: 2433 bp
ATGGAAGAGTGTTTGTTACATGGAAGATTGCACGCGACAATCTACGAAGTTGATCATCTCCATGCTGAAGGAGGTAGATCAGGTTTCTTAGGATCGATACTAGCAAATGTAGAAGAAACAATCGGTGTTGGCAAAGGAGAAACTCAGCTTTACGCTACAATCGATCTTGAGAAAGCAAGAGTTGGAAGAACGAGGAAAATAACAAAGGAGCCAAAAAACCCAAAGTGGTTTGAGTCTTTTCATATTTACTGTGGTCACATGGCTAAACATGTTATTTTCACTGTTAAGGATGCTAATCCGATTGGCGCGACGTTGATCGGAAGAGGTTATATTCCTGTGGAAGATATTTTGCATGGAGAAGAAGTTGATAGATGGGTTGATATCTTAGATAATGAGAAAAACCCAATTGCTGGAGGATCAAAGATCCATGTGAAACTCCAATATTTCGGTGTTGAAAAGGATAAAAACTGGAATCGTGGTATCAAAAGTGCTAAGTTTCCAGGAGTTCCTTACACATTCTTCTCTCAGAGGAGAGGCTGCAAAGTTTCTTTGTATCAAGATGCTCATATCCCTGGAAACTTTGTCCCGAAAATCCCTCTTGCGGGAGGGAAAAACTACGAGCCACATCGATGTTGGGAGGATATTTTCGATGCAATTACTAATGCTAAACACTTGATTTACATTACTGGTTGGTCTGTTTACACTGAGATTTCTTTAGTGAGGGACTCGAGGAGGCCAAAACAAGGAGGAGATGTGACTGTTGGTGAGCTACTCAAGAAGAAAGCTAGTGAAGGTGTTAAGGTTATTTTGCTTGTTTGGGATGACAGAACATCAGTTGATTTGCTGAAAAAAGATGGACTTATGGCTACTCATGATGAGGAAACTGAGAATTTTTTCAGAGGAACCGACGTGAACTGTATTTTGTGTCCTCGTAACCCTGATGATGGTGGAAGCATTGTCCAAAACTTGCAAATCTCAACTATGTTCACTCATCACCAAAAGATTGTTGTTGTAGACAGTGAAATGCCGAGTGGAGGATCAAGATCCAGAAGAATAGTGAGTTTTGTTGGTGGTCTTGATCTTTGTGATGGAAGATATGACACTCCGTTTCACTCCTTGTTTAGAACGTTGGACACTGCCCATCACGATGACTTCCATCAACCGAATTTCACTGGCGCTGCGATAACCAAAGGTGGTCCTAGAGAGCCATGGCATGATATTCATTGTCGTCTTGAAGGACCTATTGCTTGGGATGTCTTGTATAATTTTGAGCAGAGATGGAGTAGACAAGGCGGTAAAGATATTTTGGTTAAAATGAGAGAACTCGGAGATATTATCATCCCGCCTTCTCCCGTTCTGTTCTCAGAGGATCATGACGTGTGGAATGTTCAGTTGTTTAGATCCATTGATGGTGGAGCAGCTGCTGGATTTCCTGACTCCCCTGAAGCTGCAGCGGAAGCAGGTCTTGTGAGTGGAAAAGATAATATAATTGATAGGAGTATTCAAGATGCTTACATTCACGCGATTAGACGAGCTAAAGACTTCATCTATATCGAAAACCAGTACTTCCTTGGAAGCTCTTTTGCTTGGAGCGCGGATGGAATCAAACCAGAGGAAATCAATGCTCTGCATCTGATTCCGAAGGAGCTATCTTTGAAGATTGTTAGCAAAATCAAAGCAGGAGAGAAGTTTAAGGTCTATGTTGTTGTTCCAATGTGGCCTGAAGGGATTCCTGAGAGTGGATCAGTCCAAGCTATATTAGATTGGCAGAAAAGAACAATGGAGATGATGTACAAAGATGTAATCAAGGCACTTAGAGAAAATGGACTTGAGGGAGAGGATCCGCGAGATTATCTGACATTCTTCTGCCTCGGTAACCGAGAGGTCAAGAAAGATGGAGAGTATGAGCCTTCAGAGAAACCTGAACCGGACACTGACTATATCCGAGCACAAGAAGCACGCCGCTTCATGATCTATGTTCACACTAAAATGATGATAGTTGACGATGAGTACATTATAATCGGATCAGCAAATATCAACCAGAGGTCAATGGATGGTGCAAGAGACTCAGAGATAGCAATGGGAGGCTATCAACCATATCATCTCTCAACAAGACAACCAGCTCGAGGTCAAATCCATGGATTCCGCATGTCTTTATGGTATGAACATTTAGGAATGCTCGATGAAACATTCCTCGATCCATCGAGCCAAGAATGTATCCAGAAAGTCAACCGTGTTGCTGATAAGTATTGGGATCTTTACTCAAGTGAGTCACTTGAACATGATCTTCCTGGTCATCTACTTCGCTACCCGATCGGTATTGCTAGTGAAGGGAACATCACTGAGCTTCCCGGATGTGAATTCTTTCCCGACACAAAAGCTCGTATCCTCGGTGTCAAATCTGATTACATGCCTCCAATTCTTACAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005096 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005773 GO:0005783 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0007154 GO:0007165 GO:0007275 GO:0008047 GO:0008081 GO:0008150 GO:0008152 GO:0008289 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009791 GO:0009845 GO:0009941 GO:0009965 GO:0009966 GO:0009967 GO:0009987 GO:0010016 GO:0010033 GO:0010035 GO:0010038 GO:0010119 GO:0010358 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019752 GO:0023051 GO:0023052 GO:0023056 GO:0030054 GO:0030135 GO:0030136 GO:0030234 GO:0030695 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032501 GO:0032502 GO:0032787 GO:0032870 GO:0033993 GO:0035091 GO:0042221 GO:0042578 GO:0043085 GO:0043087 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043547 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048532 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051336 GO:0051345 GO:0051716 GO:0055044 GO:0060589 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0090351 GO:0097305 GO:0097306 GO:0097708 GO:0098772 GO:0099402 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1901981 GO:1902936 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231
Pfam Domains
Protein Families
C2 PLD_C PLDc

Protein Analysis

810

Amino Acids

91.6

Weight (kDa)

5.82

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 45 - 127 3.2e-07 C2 domain
PLDc PF00614 328 - 365 1.1e-06 Phospholipase D Active site motif
PLDc_2 PF13091 656 - 689 3.9e-06 PLD-like domain
PLDc PF00614 658 - 683 3e-08 Phospholipase D Active site motif
PLD_C PF12357 728 - 799 2.1e-28 Phospholipase D C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2018
Acc36I ACCTGC 1 cut(s) 1451
AccB7I CCANNNNNTGG 1 cut(s) 410
AccI GTMKAC 2 cut(s) 1014, 1285
AccII CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
AciI CCGC 8 cut(s) 602, 1293, 1343, 1454, 1595, 1844, 1963, 2146
AclI AACGTT 1 cut(s) 1127
AcsI RAATTY 3 cut(s) 889, 1165, 2360
AcuI CTGAAG 4 cut(s) 87, 1464, 1743, 1896
AfaI GTAC 3 cut(s) 1565, 1793, 2012
AfiI CCNNNNNNNGG 8 cut(s) 410, 601, 602, 743, 944, 1034, 1739, 2126
AflIII ACRYGT 2 cut(s) 268, 1377
AgsI TTSAA 5 cut(s) 455, 1235, 1505, 1657, 2279
AjiI CACGTC 2 cut(s) 910, 1378
AjnI CCWGG 3 cut(s) 499, 571, 2290
Alw21I GWGCWC 1 cut(s) 1951
Alw26I GTCTC 1 cut(s) 2058
AlwNI CAGNNNCTG 1 cut(s) 1424
Ama87I CYCGRG 2 cut(s) 727, 2124
AoxI GGCC 2 cut(s) 734, 1718
ApeKI GCWGC 6 cut(s) 537, 1178, 1418, 1421, 1448, 1451
ApoI RAATTY 3 cut(s) 889, 1165, 2360
Asp700I GAANNNNTTC 3 cut(s) 893, 1728, 2364
AspLEI GCGC 3 cut(s) 308, 1178, 1595
AspS9I GGNCC 2 cut(s) 1196, 1238
AsuC2I CCSGG 1 cut(s) 2352
AsuHPI GGTGA 2 cut(s) 776, 986
AvaI CYCGRG 2 cut(s) 727, 2124
AvaII GGWCC 2 cut(s) 1196, 1238
BamHI GGATCC 1 cut(s) 1840
Bbv12I GWGCWC 1 cut(s) 1951
BbvI GCAGC 6 cut(s) 524, 1165, 1408, 1430, 1435, 1463
BciT130I CCWGG 3 cut(s) 501, 573, 2292
BciVI GTATCC 2 cut(s) 2228, 2396
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 1 cut(s) 2352
BcoDI GTCTC 1 cut(s) 2058
BfaI CTAG 4 cut(s) 101, 786, 1200, 2325
BfmI CTRYAG 1 cut(s) 1449
BfoI RGCGCY 1 cut(s) 1179
BfuAI ACCTGC 1 cut(s) 1451
BfuI GTATCC 2 cut(s) 2228, 2396
BisI GCNGC 7 cut(s) 538, 1179, 1419, 1422, 1449, 1452, 1963
BlsI GCNGC 7 cut(s) 539, 1180, 1420, 1423, 1450, 1453, 1964
BmcAI AGTACT 1 cut(s) 1565
Bme1390I CCNGG 4 cut(s) 501, 573, 2292, 2352
Bme18I GGWCC 2 cut(s) 1196, 1238
BmeT110I CYCGRG 2 cut(s) 727, 2124
BmgBI CACGTC 2 cut(s) 910, 1378
BmgT120I GGNCC 2 cut(s) 1196, 1238
BmiI GGNNCC 3 cut(s) 210, 904, 1842
BmrFI CCNGG 4 cut(s) 501, 573, 2292, 2352
BmsI GCATC 5 cut(s) 280, 550, 640, 1498, 1639
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 4 cut(s) 188, 758, 1850, 2248
BpuMI CCSGG 1 cut(s) 2352
Bsa29I ATCGAT 3 cut(s) 95, 162, 628
BsaBI GATNNNNATC 2 cut(s) 294, 1042
BsaJI CCNNGG 6 cut(s) 571, 1208, 1570, 1870, 2137, 2389
BsaWI WCCGGW 1 cut(s) 1927
BsaXI ACNNNNNCTCC 6 cut(s) 348, 378, 738, 768, 1775, 1805
Bsc4I CCNNNNNNNGG 8 cut(s) 410, 601, 602, 743, 944, 1034, 1739, 2126
Bse1I ACTGG 4 cut(s) 473, 691, 1177, 1561
Bse3DI GCAATG 1 cut(s) 2085
Bse8I GATNNNNATC 2 cut(s) 294, 1042
BseBI CCWGG 3 cut(s) 501, 573, 2292
BseCI ATCGAT 3 cut(s) 95, 162, 628
BseDI CCNNGG 6 cut(s) 571, 1208, 1570, 1870, 2137, 2389
BseGI GGATG 7 cut(s) 295, 826, 1258, 1338, 1603, 2059, 2360
BseJI GATNNNNATC 2 cut(s) 294, 1042
BseLI CCNNNNNNNGG 8 cut(s) 410, 601, 602, 743, 944, 1034, 1739, 2126
BseMI GCAATG 1 cut(s) 2085
BseMII CTCAG 8 cut(s) 158, 539, 696, 876, 1377, 1725, 2082, 2334
BseNI ACTGG 4 cut(s) 473, 691, 1177, 1561
BseRI GAGGAG 3 cut(s) 544, 745, 762
BseXI GCAGC 6 cut(s) 524, 1165, 1408, 1430, 1435, 1463
Bsh1236I CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
Bsh1285I CGRYCG 1 cut(s) 2316
BshFI GGCC 2 cut(s) 736, 1720
BshVI ATCGAT 3 cut(s) 95, 162, 628
BsiEI CGRYCG 1 cut(s) 2316
BsiHKAI GWGCWC 1 cut(s) 1951
BsiHKCI CYCGRG 2 cut(s) 727, 2124
BsiSI CCGG 2 cut(s) 1928, 2352
BslFI GGGAC 2 cut(s) 569, 737
BslI CCNNNNNNNGG 8 cut(s) 410, 601, 602, 743, 944, 1034, 1739, 2126
BsmAI GTCTC 1 cut(s) 2058
BsmFI GGGAC 2 cut(s) 569, 737
BsmI GAATGC 1 cut(s) 2181
BsnI GGCC 2 cut(s) 736, 1720
BsoBI CYCGRG 2 cut(s) 727, 2124
Bsp1286I GDGCHC 1 cut(s) 1951
Bsp1407I TGTACA 1 cut(s) 1791
Bsp19I CCATGG 2 cut(s) 1208, 2137
BspACI CCGC 8 cut(s) 602, 1293, 1343, 1454, 1595, 1844, 1963, 2146
BspANI GGCC 2 cut(s) 736, 1720
BspCNI CTCAG 8 cut(s) 157, 538, 697, 877, 1376, 1726, 2081, 2335
BspDI ATCGAT 3 cut(s) 95, 162, 628
BspFNI CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
BspHI TCATGA 3 cut(s) 871, 1372, 1968
BspLI GGNNCC 3 cut(s) 210, 904, 1842
BspMAI CTGCAG 1 cut(s) 1453
BspMI ACCTGC 1 cut(s) 1451
BsrDI GCAATG 1 cut(s) 2085
BsrGI TGTACA 1 cut(s) 1791
BsrI ACTGG 4 cut(s) 473, 691, 1177, 1561
BssECI CCNNGG 6 cut(s) 571, 1208, 1570, 1870, 2137, 2389
BssT1I CCWWGG 3 cut(s) 1208, 1570, 2137
Bst2UI CCWGG 3 cut(s) 501, 573, 2292
Bst4CI ACNGT 6 cut(s) 251, 283, 760, 917, 1019, 2234
Bst6I CTCTTC 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 1791
BstC8I GCNNGC 2 cut(s) 33, 1960
BstDSI CCRYGG 2 cut(s) 1208, 2137
BstEII GGTNACC 1 cut(s) 1874
BstF5I GGATG 7 cut(s) 295, 826, 1258, 1338, 1603, 2059, 2360
BstFNI CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
BstH2I RGCGCY 1 cut(s) 1179
BstHHI GCGC 3 cut(s) 308, 1178, 1595
BstMAI GTCTC 1 cut(s) 2058
BstMCI CGRYCG 1 cut(s) 2316
BstMWI GCNNNNNNNGC 3 cut(s) 1457, 1592, 1955
BstNI CCWGG 3 cut(s) 501, 573, 2292
BstNSI RCATGY 3 cut(s) 272, 2152, 2413
BstPI GGTNACC 1 cut(s) 1874
BstSCI CCNGG 4 cut(s) 499, 571, 2290, 2350
BstSFI CTRYAG 1 cut(s) 1449
BstUI CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
BstV1I GCAGC 6 cut(s) 524, 1165, 1408, 1430, 1435, 1463
BstX2I RGATCY 5 cut(s) 423, 1043, 1400, 1840, 2254
BstYI RGATCY 5 cut(s) 423, 1043, 1400, 1840, 2254
Bsu15I ATCGAT 3 cut(s) 95, 162, 628
BsuI GTATCC 2 cut(s) 2228, 2396
BsuRI GGCC 2 cut(s) 736, 1720
BsuTUI ATCGAT 3 cut(s) 95, 162, 628
BtgI CCRYGG 2 cut(s) 1208, 2137
BtrI CACGTC 2 cut(s) 910, 1378
BtsCI GGATG 7 cut(s) 295, 826, 1258, 1338, 1603, 2059, 2360
BtsI GCAGTG 1 cut(s) 1134
BtsIMutI CAGTG 7 cut(s) 279, 702, 1024, 1134, 1170, 1932, 2340
BveI ACCTGC 1 cut(s) 1451
Cac8I GCNNGC 2 cut(s) 33, 1960
CaiI CAGNNNCTG 1 cut(s) 1424
CciI TCATGA 3 cut(s) 871, 1372, 1968
CfoI GCGC 3 cut(s) 308, 1178, 1595
Cfr13I GGNCC 2 cut(s) 1196, 1238
ClaI ATCGAT 3 cut(s) 95, 162, 628
Csp6I GTAC 3 cut(s) 1564, 1792, 2011
CviQI GTAC 3 cut(s) 1564, 1792, 2011
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 3 cut(s) 1208, 1570, 2137
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 2 cut(s) 1196, 1238
Eco57I CTGAAG 4 cut(s) 87, 1464, 1743, 1896
Eco88I CYCGRG 2 cut(s) 727, 2124
Eco91I GGTNACC 1 cut(s) 1874
EcoO109I RGGNCCY 1 cut(s) 1238
EcoO65I GGTNACC 1 cut(s) 1874
EcoRI GAATTC 1 cut(s) 2360
EcoRII CCWGG 3 cut(s) 499, 571, 2290
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 3 cut(s) 1208, 1570, 2137
ErhI CCWWGG 3 cut(s) 1208, 1570, 2137
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 1449, 1481
FaqI GGGAC 2 cut(s) 569, 737
FauI CCCGC 2 cut(s) 595, 1350
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 2 cut(s) 1014, 1285
Fnu4HI GCNGC 7 cut(s) 538, 1179, 1419, 1422, 1449, 1452, 1963
FokI GGATG 7 cut(s) 302, 833, 1265, 1325, 1610, 2066, 2367
Fsp4HI GCNGC 7 cut(s) 538, 1179, 1419, 1422, 1449, 1452, 1963
FspBI CTAG 4 cut(s) 101, 786, 1200, 2325
GlaI GCGC 3 cut(s) 307, 1177, 1594
GluI GCNGC 7 cut(s) 538, 1179, 1419, 1422, 1449, 1452, 1963
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 1179
HaeIII GGCC 2 cut(s) 736, 1720
HapII CCGG 2 cut(s) 1928, 2352
HhaI GCGC 3 cut(s) 308, 1178, 1595
Hin6I GCGC 3 cut(s) 306, 1176, 1593
HinP1I GCGC 3 cut(s) 306, 1176, 1593
HincII GTYRAC 2 cut(s) 2002, 2230
HindII GTYRAC 2 cut(s) 2002, 2230
HpaII CCGG 2 cut(s) 1928, 2352
HphI GGTGA 2 cut(s) 776, 986
Hpy166II GTNNAC 8 cut(s) 700, 913, 987, 1015, 1286, 1981, 2002, 2230
Hpy8I GTNNAC 8 cut(s) 700, 913, 987, 1015, 1286, 1981, 2002, 2230
Hpy99I CGWCG 2 cut(s) 313, 911
HpyAV CCTTC 8 cut(s) 62, 785, 1229, 1356, 1636, 1718, 1920, 2324
HpyCH4III ACNGT 6 cut(s) 251, 283, 760, 917, 1019, 2234
HpyCH4IV ACGT 4 cut(s) 311, 909, 1127, 1377
HpyCH4V TGCA 8 cut(s) 31, 352, 540, 653, 970, 1451, 1630, 2060
HpyF10VI GCNNNNNNNGC 3 cut(s) 1457, 1592, 1955
HpySE526I ACGT 4 cut(s) 311, 909, 1127, 1377
HspAI GCGC 3 cut(s) 306, 1176, 1593
Ksp22I TGATCA 1 cut(s) 52
LmnI GCTCC 4 cut(s) 208, 1415, 1590, 1645
Lsp1109I GCAGC 6 cut(s) 524, 1165, 1408, 1430, 1435, 1463
LweI GCATC 5 cut(s) 280, 550, 640, 1498, 1639
MaeI CTAG 4 cut(s) 101, 786, 1200, 2325
MaeII ACGT 4 cut(s) 311, 909, 1127, 1377
MaeIII GTNAC 6 cut(s) 15, 254, 754, 932, 1874, 2271
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 5 cut(s) 423, 1043, 1400, 1840, 2254
MhlI GDGCHC 1 cut(s) 1951
MluCI AATT 8 cut(s) 405, 654, 889, 1165, 1264, 1488, 2360, 2418
MlyI GAGTC 5 cut(s) 242, 719, 1430, 2060, 2279
MmeI TCCRAC 2 cut(s) 163, 1110
MroXI GAANNNNTTC 3 cut(s) 893, 1728, 2364
MseI TTAA 4 cut(s) 285, 798, 1311, 1692
MslI CAYNNNNRTG 4 cut(s) 60, 628, 1146, 1988
MspA1I CMGCKG 2 cut(s) 1421, 1454
MspI CCGG 2 cut(s) 1928, 2352
MspR9I CCNGG 4 cut(s) 501, 573, 2292, 2352
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 2181
MvaI CCWGG 3 cut(s) 501, 573, 2292
MvnI CGCG 5 cut(s) 35, 308, 1523, 1595, 1846
MwoI GCNNNNNNNGC 3 cut(s) 1457, 1592, 1955
NciI CCSGG 1 cut(s) 2352
NcoI CCATGG 2 cut(s) 1208, 2137
NlaIV GGNNCC 3 cut(s) 210, 904, 1842
NmeAIII GCCGAG 1 cut(s) 1053
NmuCI GTSAC 3 cut(s) 254, 754, 2271
NspI RCATGY 3 cut(s) 272, 2152, 2413
PaeR7I CTCGAG 2 cut(s) 727, 2124
PagI TCATGA 3 cut(s) 871, 1372, 1968
PciI ACATGT 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 2181
PdmI GAANNNNTTC 3 cut(s) 893, 1728, 2364
PfeI GAWTC 5 cut(s) 472, 1602, 1636, 1729, 2142
PflMI CCANNNNNTGG 1 cut(s) 410
PfoI TCCNGGA 2 cut(s) 499, 2350
PkrI GCNGC 7 cut(s) 539, 1180, 1420, 1423, 1450, 1453, 1964
Ple19I CGATCG 1 cut(s) 2316
PleI GAGTC 5 cut(s) 241, 719, 1430, 2060, 2278
PpsI GAGTC 5 cut(s) 241, 719, 1430, 2060, 2278
PpuMI RGGWCCY 1 cut(s) 1238
PscI ACATGT 1 cut(s) 268
PsiI TTATAA 1 cut(s) 2018
Psp1406I AACGTT 1 cut(s) 1127
Psp5II RGGWCCY 1 cut(s) 1238
Psp6I CCWGG 3 cut(s) 499, 571, 2290
PspEI GGTNACC 1 cut(s) 1874
PspGI CCWGG 3 cut(s) 499, 571, 2290
PspN4I GGNNCC 3 cut(s) 210, 904, 1842
PspPI GGNCC 2 cut(s) 1196, 1238
PspPPI RGGWCCY 1 cut(s) 1238
PspXI VCTCGAGB 2 cut(s) 727, 2124
PstI CTGCAG 1 cut(s) 1453
PstNI CAGNNNCTG 1 cut(s) 1424
PsuI RGATCY 5 cut(s) 423, 1043, 1400, 1840, 2254
PvuI CGATCG 1 cut(s) 2316
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 3 cut(s) 1565, 1793, 2012
RsaNI GTAC 3 cut(s) 1564, 1792, 2011
RseI CAYNNNNRTG 4 cut(s) 60, 628, 1146, 1988
SaqAI TTAA 4 cut(s) 285, 798, 1311, 1692
SatI GCNGC 7 cut(s) 538, 1179, 1419, 1422, 1449, 1452, 1963
Sau96I GGNCC 2 cut(s) 1196, 1238
ScaI AGTACT 1 cut(s) 1565
SchI GAGTC 5 cut(s) 242, 719, 1430, 2060, 2279
ScrFI CCNGG 4 cut(s) 501, 573, 2292, 2352
SduI GDGCHC 1 cut(s) 1951
SfaNI GCATC 5 cut(s) 280, 550, 640, 1498, 1639
SfcI CTRYAG 1 cut(s) 1449
Sfr274I CTCGAG 2 cut(s) 727, 2124
SinI GGWCC 2 cut(s) 1196, 1238
SlaI CTCGAG 2 cut(s) 727, 2124
SmiMI CAYNNNNRTG 4 cut(s) 60, 628, 1146, 1988
SmlI CTYRAG 6 cut(s) 167, 727, 773, 1829, 2124, 2263
SmoI CTYRAG 6 cut(s) 167, 727, 773, 1829, 2124, 2263
Sse9I AATT 8 cut(s) 405, 654, 889, 1165, 1264, 1488, 2360, 2418
SsiI CCGC 8 cut(s) 602, 1293, 1343, 1454, 1595, 1844, 1963, 2146
SspI AATATT 1 cut(s) 443
SspMI CTAG 4 cut(s) 101, 786, 1200, 2325
StyD4I CCNGG 4 cut(s) 499, 571, 2290, 2350
StyI CCWWGG 3 cut(s) 1208, 1570, 2137
TaaI ACNGT 6 cut(s) 251, 283, 760, 917, 1019, 2234
TaiI ACGT 4 cut(s) 314, 912, 1130, 1380
TasI AATT 8 cut(s) 405, 654, 889, 1165, 1264, 1488, 2360, 2418
TatI WGTACW 3 cut(s) 1563, 1791, 2010
TauI GCSGC 1 cut(s) 1965
TfiI GAWTC 5 cut(s) 472, 1602, 1636, 1729, 2142
Tru1I TTAA 4 cut(s) 285, 798, 1311, 1692
Tru9I TTAA 4 cut(s) 285, 798, 1311, 1692
TscAI CASTG 7 cut(s) 286, 709, 1024, 1141, 1177, 1939, 2347
TseFI GTSAC 3 cut(s) 254, 754, 2271
TseI GCWGC 6 cut(s) 537, 1178, 1418, 1421, 1448, 1451
Tsp45I GTSAC 3 cut(s) 254, 754, 2271
TspDTI ATGAA 6 cut(s) 230, 1211, 1534, 1957, 2178, 2199
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 7 cut(s) 286, 709, 1024, 1141, 1177, 1939, 2347
Van91I CCANNNNNTGG 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 1196, 1238
XapI RAATTY 3 cut(s) 889, 1165, 2360
XceI RCATGY 3 cut(s) 272, 2152, 2413
XcmI CCANNNNNNNNNTGG 2 cut(s) 630, 1568
XhoI CTCGAG 2 cut(s) 727, 2124
XmiI GTMKAC 2 cut(s) 1014, 1285
XmnI GAANNNNTTC 3 cut(s) 893, 1728, 2364
XspI CTAG 4 cut(s) 101, 786, 1200, 2325
ZrmI AGTACT 1 cut(s) 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.