Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005265

Physical Location & Seq

Genomic Coordinates Forward (+)

25393822 .. 25398354

Gene Structure 4533 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1209200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGGACGCTTCACGCTACGATCTATGAGGTTGATAAGTTGCATAGTAGTAGTGGGAATTTCCTCCGCAAGTTATATGAATTTTCATATTGCAGGAAAAATTGAGGAGACTGTTGGTATTGGCAAGGGAATTAGTAAACTCTATGCAACCGTTGATCTGGAAAGGGCTAGGGTTGGTAGGACCAGGGTTATAGAGAAAGAGCCCTCTAATCCCAGGTGGTATGAGTCTTTCCACATTTACTGTGCTCATACTGCTGCAAATGTTATATTTACTGTCAAGGAGTCTAATCCTATTGGGGCATCATTAATTGGAAGAGCATATGTACCTGTTCAAGAACTCATAGAGGGGGAAGAAGTGGATCAATGGGCTGAAATTCTGGATGAAAAAAAGAACCCTGTCCATGGAAATCCTAAAATACATGTGAAACTACAATTTTTCCATGTTACAAAGGACCGCAGTTGGGGTCTGGGTATAAGGAGTCCTAAGTTTCCTGGAGTTCCGTTTACATTTTTCTCACAGAGACAAGGGTGTAAGGTTTCCCTTTACCAAGATGCTCATATCCCAGATAAATTTATTCCTAAAATTCCTCTTGCTGGAGGCAAGCGTTACGAGCCCCACAGATGCTGGGAAGACATCTTTGATGCAATTACTAATGCAAAGCACCTGATCTACATTACTGGTTGGTCAGTTTATACTGAAATTTCCTTGGTAAGGGACTCAAGAAGGCCAAAGCCTGGTGGAGACGTCACCATTGGTGAATTGCTTAAAAAGAAAGCAAGTGAAGGTGTTAGGGTTCTTATGCTTGTTTGGGATGACAGAACCTCTGTTGGTTTATTGAAAAAAGATGGACTGATGGCCACTCATGATGAAGACACTGCAAAGTTCTTCCAGGGTACTGATGTGCATTGTGTCTTATGCCCCCGTAATCCTGATGGTGGCGGGAGCATTATTCAGGGTGCACAAGTCTCTACCATGTTCACTCATCACCAGAAGATTGTGGTTGTGGACCATGACATGCCAAATGGAGAATCAGAGAGGAGAAGAATTGTTAGTTTTGTTGGGGGTCTTGATCTGTGTGATGGAAGATATGATACCCCCTTCCATTCACTTTTCAGGACATTGGATACTGCACACCATGATGATTTTCATCAGCCAAATTTTACTGGTGCCTCAATTACAAAAGGTGGTCCAAGGGAACCGTGGCATGATATCCACTCTCGACTGGAAGGGCCTATTGCTTGGGATGTTTTGTTTAACTTTGAGCAGAGGTGGAGAAAGCAAGGTGGTAAAGATTTACTTGTTCAGCTTAGAGAGCTGGAGGAAATCATCATTCCCCCTTCTCCTGTTATGTTCCCAGATGACCATGAGACATGGAATGTTCAGTTATTCAGATCAATTGATGGAGGAGCTGCTTTTGGCTTCCCAGATACGCCTGAAGATGCAGCTCGAGCTGGGCTTGTTAGTGGGAAGGATAACATCATTGACCGAAGCATCCAGGATGCTTATATTCATGCTATTCGCCGCGCAAAGAATTTCATCTATATTGAAAATCAGTACTTTCTAGGGAGCTCTTTTGCCTGGGAGGCTGATGGTATAAAGCCTGAGGACATTGGCGCTTTGCATGTAATTCCAAGGGAGCTTTCACTTAAGATTGCTAGCAAGATTGAAGCAGGGGAGAGGTTTACTGTCTATGTCGTTGTTCCAATGTGGCCAGAGGGGATCCCAGAGTCAGGATCTGTTCAGGCAATATTAGACTGGCAGAGGAGGACTATGAATATGATGTATGCAGATGTTAAGCGTGCATTAGATGCACAAGGTCTCGAGGAGGATCCTCGGACCTACTTGACATTTTTCTGCCTTGGGAATCGGGAGGTAAAGAAGCCTGGGGAATATGAACCCTCAGAGACACCAGAAGCAGATTCAGACTATCAAAGAGCTCAGGCAGCCCGACGCTTCATGATTTATGTTCATGCCAAGATGATGATAGTTGACGATGAATACATAATTATTGGATCTGCCAACATCAACCAGAGATCAATGGATGGTGCCAGAGACTCTGAAATAGCAATGGGAGCCTACCAACCATATCATCTGTCTGTCAGGGAACCAGCACGTGGTCAGATCCATGGTTTCCGTATGGCACTATGGTATGAGCACCTTGGCATGCTTGATGAGGCCTTCCTTCAGCCAGAAAGAATTGAATGTATTTCAAAGGTGAACCAGATTGCTGACAAATACTGGGATCTCTATTCAAGTGAGACTCTTGAACATGATTTACCTGGTCACTTGCTTCGATATCCAGTTGGTATTACCAGCGAAGGAGTTGTCGATGAGTTGCCAGGATTTGAATTCTTCCCTGACACCAAAGCTCGTATTCTTGGTGCCAAATCCGACTACCTTCCTCCGATCCTTACTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

807

Amino Acids

91.27

Weight (kDa)

5.92

Isoelectric Point (pI)

41.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	42 - 124	1.7e-09	C2 domain
PLDc	PF00614	325 - 362	8e-07	Phospholipase D Active site motif
PLDc_2	PF13091	653 - 686	1.9e-06	PLD-like domain
PLDc	PF00614	654 - 679	3.9e-08	Phospholipase D Active site motif
PLD_C	PF12357	724 - 796	2.4e-28	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52570 AT1G52570 AT1G52570 AT3G15730
fragaria_vesca	FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920
malus_domestica	MD00G1090900.v1.1 MD17G1209200.v1.1
prunus_persica	Prupe.3G084800_v2.0.a1 Prupe.3G084800_v2.0.a1 Prupe.3G084800_v2.0.a1
pyrus_communis	pycom09g14080
rosa_chinensis	RchiOBHm_Chr2g0135901
rosa_laevigata	RLG00000019489
rosa_multiflora	Rmu_sc0001825.1_g000006 Rmu_sc0007848.1_g000004
rosa_roxburghii	Rroxscaffold_2G00108790
rosa_rugosa	Rorug02G0326700 Rorug02G0326700 Rorug02G0326700
rosa_samantha	Rh2AG378300 Rh2BG384800 Rh2CG364100 Rh2DG401200
rosa_wichuraiana	Rw2G030820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	747
AccB1I	GGYRCC	3 cut(s)	1166, 2045, 2381
AccB7I	CCANNNNNTGG	2 cut(s)	734, 2114
AccII	CGCG	1 cut(s)	1524
AciI	CCGC	4 cut(s)	66, 454, 939, 1522
AcoI	YGGCCR	2 cut(s)	855, 1709
AcsI	RAATTY	9 cut(s)	57, 79, 372, 569, 582, 699, 1156, 1531, 2348
AcuI	CTGAAG	2 cut(s)	1455, 2168
AcvI	CACGTG	1 cut(s)	2114
AcyI	GRCGYC	1 cut(s)	744
AfaI	GTAC	3 cut(s)	324, 895, 1556
AfiI	CCNNNNNNNGG	8 cut(s)	401, 460, 593, 711, 734, 935, 1730, 2114
AflII	CTTAAG	1 cut(s)	1646
AflIII	ACRYGT	1 cut(s)	418
AgsI	TTSAA	9 cut(s)	332, 838, 1547, 1667, 2201, 2211, 2253, 2267, 2348
AjuI	GAANNNNNNNTTGG	2 cut(s)	1624, 1656
AluBI	AGCT	9 cut(s)	1306, 1315, 1409, 1445, 1451, 1569, 1639, 1937, 2369
AluI	AGCT	9 cut(s)	1306, 1315, 1409, 1445, 1451, 1569, 1639, 1937, 2369
Alw21I	GWGCWC	5 cut(s)	247, 961, 1571, 1939, 2157
Alw26I	GTCTC	9 cut(s)	101, 514, 735, 970, 1361, 1823, 1898, 2046, 2252
Alw44I	GTGCAC	1 cut(s)	957
AlwNI	CAGNNNCTG	2 cut(s)	624, 1736
Ama87I	CYCGRG	2 cut(s)	1446, 1820
AoxI	GGCC	5 cut(s)	725, 855, 1229, 1709, 2175
ApaLI	GTGCAC	1 cut(s)	957
ApeKI	GCWGC	4 cut(s)	254, 1409, 1442, 1943
ApoI	RAATTY	9 cut(s)	57, 79, 372, 569, 582, 699, 1156, 1531, 2348
ArsI	GACNNNNNNTTYG	2 cut(s)	2310, 2342
AseI	ATTAAT	1 cut(s)	305
AspLEI	GCGC	2 cut(s)	1526, 1616
AspS9I	GGNCC	6 cut(s)	180, 451, 1006, 1187, 1229, 1836
AsuHPI	GGTGA	4 cut(s)	739, 767, 977, 2227
AsuNHI	GCTAGC	1 cut(s)	1655
AvaI	CYCGRG	2 cut(s)	1446, 1820
AvaII	GGWCC	5 cut(s)	180, 451, 1006, 1187, 1836
AxyI	CCTNAGG	1 cut(s)	1602
BaeGI	GKGCMC	1 cut(s)	961
BaeI	ACNNNNGTAYC	2 cut(s)	885, 918
BalI	TGGCCA	2 cut(s)	857, 1711
BamHI	GGATCC	2 cut(s)	1719, 1828
BanI	GGYRCC	3 cut(s)	1166, 2045, 2381
BanII	GRGCYC	4 cut(s)	204, 615, 1571, 1939
BarI	GAAGNNNNNNTAC	2 cut(s)	1075, 1107
BbrPI	CACGTG	1 cut(s)	2114
BbsI	GAAGAC	2 cut(s)	636, 876
Bbv12I	GWGCWC	5 cut(s)	247, 961, 1571, 1939, 2157
BbvI	GCAGC	4 cut(s)	241, 1396, 1454, 1955
BccI	CCATC	7 cut(s)	839, 847, 926, 1073, 1394, 1583, 2036
BciVI	GTATCC	1 cut(s)	1117
BcoDI	GTCTC	9 cut(s)	101, 514, 735, 970, 1361, 1823, 1898, 2046, 2252
BfaI	CTAG	3 cut(s)	168, 1562, 1656
BfoI	RGCGCY	1 cut(s)	1617
BfrI	CTTAAG	1 cut(s)	1646
BfuI	GTATCC	1 cut(s)	1117
BglI	GCCNNNNNGGC	1 cut(s)	1583
BisI	GCNGC	5 cut(s)	255, 1410, 1443, 1522, 1944
BlsI	GCNGC	5 cut(s)	256, 1411, 1444, 1523, 1945
BmcAI	AGTACT	1 cut(s)	1556
Bme18I	GGWCC	5 cut(s)	180, 451, 1006, 1187, 1836
BmeT110I	CYCGRG	2 cut(s)	1446, 1820
BmgT120I	GGNCC	6 cut(s)	180, 451, 1006, 1187, 1229, 1836
BmiI	GGNNCC	8 cut(s)	1168, 1197, 1721, 1830, 2047, 2074, 2106, 2383
BmrI	ACTGGG	1 cut(s)	2248
BmsI	GCATC	8 cut(s)	308, 541, 611, 631, 1429, 1489, 1500, 1798
BmtI	GCTAGC	1 cut(s)	1659
BmuI	ACTGGG	1 cut(s)	2248
BpiI	GAAGAC	2 cut(s)	636, 876
BpmI	CTGGAG	3 cut(s)	513, 615, 1337
Bpu10I	CCTNAGC	1 cut(s)	1938
BpuEI	CTTGAG	1 cut(s)	703
BsaAI	YACGTR	1 cut(s)	2114
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaHI	GRCGYC	1 cut(s)	744
BsaI	GGTCTC	1 cut(s)	1823
Bsc4I	CCNNNNNNNGG	8 cut(s)	401, 460, 593, 711, 734, 935, 1730, 2114
Bse1I	ACTGG	6 cut(s)	682, 1168, 1227, 1760, 2243, 2300
Bse21I	CCTNAGG	1 cut(s)	1602
Bse3DI	GCAATG	1 cut(s)	2073
Bse8I	GATNNNNATC	1 cut(s)	1146
BseGI	GGATG	6 cut(s)	385, 817, 1249, 1491, 1504, 2047
BseJI	GATNNNNATC	1 cut(s)	1146
BseLI	CCNNNNNNNGG	8 cut(s)	401, 460, 593, 711, 734, 935, 1730, 2114
BseMI	GCAATG	1 cut(s)	2073
BseMII	CTCAG	3 cut(s)	1593, 1914, 1952
BseNI	ACTGG	6 cut(s)	682, 1168, 1227, 1760, 2243, 2300
BseRI	GAGGAG	5 cut(s)	119, 1051, 1419, 1777, 1838
BseSI	GKGCMC	1 cut(s)	961
BseXI	GCAGC	4 cut(s)	241, 1396, 1454, 1955
BseYI	CCCAGC	2 cut(s)	624, 1451
BsgI	GTGCAG	1 cut(s)	1113
Bsh1236I	CGCG	1 cut(s)	1524
BshFI	GGCC	5 cut(s)	727, 857, 1231, 1711, 2177
BshNI	GGYRCC	3 cut(s)	1166, 2045, 2381
BsiHKAI	GWGCWC	5 cut(s)	247, 961, 1571, 1939, 2157
BsiHKCI	CYCGRG	2 cut(s)	1446, 1820
BslFI	GGGAC	2 cut(s)	18, 728
BslI	CCNNNNNNNGG	8 cut(s)	401, 460, 593, 711, 734, 935, 1730, 2114
BsmAI	GTCTC	9 cut(s)	101, 514, 735, 970, 1361, 1823, 1898, 2046, 2252
BsmBI	CGTCTC	1 cut(s)	735
BsmFI	GGGAC	2 cut(s)	18, 728
BsnI	GGCC	5 cut(s)	727, 857, 1231, 1711, 2177
Bso31I	GGTCTC	1 cut(s)	1823
BsoBI	CYCGRG	2 cut(s)	1446, 1820
Bsp1286I	GDGCHC	7 cut(s)	204, 247, 615, 961, 1571, 1939, 2157
Bsp19I	CCATGG	2 cut(s)	400, 2125
BspACI	CCGC	4 cut(s)	66, 454, 939, 1522
BspANI	GGCC	5 cut(s)	727, 857, 1231, 1711, 2177
BspCNI	CTCAG	3 cut(s)	1594, 1913, 1951
BspFNI	CGCG	1 cut(s)	1524
BspHI	TCATGA	2 cut(s)	862, 1956
BspLI	GGNNCC	8 cut(s)	1168, 1197, 1721, 1830, 2047, 2074, 2106, 2383
BspOI	GCTAGC	1 cut(s)	1659
BspQI	GCTCTTC	1 cut(s)	307
BspT107I	GGYRCC	3 cut(s)	1166, 2045, 2381
BspTI	CTTAAG	1 cut(s)	1646
BspTNI	GGTCTC	1 cut(s)	1823
BsrDI	GCAATG	1 cut(s)	2073
BsrI	ACTGG	6 cut(s)	682, 1168, 1227, 1760, 2243, 2300
BssNI	GRCGYC	1 cut(s)	744
BssT1I	CCWWGG	7 cut(s)	400, 705, 1190, 1631, 1858, 2125, 2158
Bst4CI	ACNGT	6 cut(s)	112, 151, 242, 274, 1200, 1687
Bst6I	CTCTTC	1 cut(s)	307
BstACI	GRCGYC	1 cut(s)	744
BstAFI	CTTAAG	1 cut(s)	1646
BstAPI	GCANNNNNTGC	1 cut(s)	1808
BstBAI	YACGTR	1 cut(s)	2114
BstC8I	GCNNGC	4 cut(s)	602, 1657, 1800, 2165
BstDEI	CTNAG	5 cut(s)	483, 1307, 1602, 1900, 1938
BstDSI	CCRYGG	3 cut(s)	400, 1199, 2125
BstENI	CCTNNNNNAGG	1 cut(s)	709
BstF5I	GGATG	6 cut(s)	385, 817, 1249, 1491, 1504, 2047
BstFNI	CGCG	1 cut(s)	1524
BstH2I	RGCGCY	1 cut(s)	1617
BstHHI	GCGC	2 cut(s)	1526, 1616
BstMAI	GTCTC	9 cut(s)	101, 514, 735, 970, 1361, 1823, 1898, 2046, 2252
BstMWI	GCNNNNNNNGC	8 cut(s)	251, 610, 1312, 1448, 1583, 1808, 1943, 2072
BstNSI	RCATGY	4 cut(s)	422, 1018, 1625, 2167
BstSLI	GKGCMC	1 cut(s)	961
BstUI	CGCG	1 cut(s)	1524
BstV1I	GCAGC	4 cut(s)	241, 1396, 1454, 1955
BstV2I	GAAGAC	2 cut(s)	636, 876
BstX2I	RGATCY	6 cut(s)	1719, 1733, 1828, 2012, 2121, 2242
BstYI	RGATCY	6 cut(s)	1719, 1733, 1828, 2012, 2121, 2242
Bsu36I	CCTNAGG	1 cut(s)	1602
BsuI	GTATCC	1 cut(s)	1117
BsuRI	GGCC	5 cut(s)	727, 857, 1231, 1711, 2177
BtgI	CCRYGG	3 cut(s)	400, 1199, 2125
BtsCI	GGATG	6 cut(s)	385, 817, 1249, 1491, 1504, 2047
BtsI	GCAGTG	1 cut(s)	873
BtsIMutI	CAGTG	1 cut(s)	873
Cac8I	GCNNGC	4 cut(s)	602, 1657, 1800, 2165
CaiI	CAGNNNCTG	2 cut(s)	624, 1736
CciI	TCATGA	2 cut(s)	862, 1956
CfoI	GCGC	2 cut(s)	1526, 1616
Cfr13I	GGNCC	6 cut(s)	180, 451, 1006, 1187, 1229, 1836
CseI	GACGC	2 cut(s)	15, 1959
CsiI	ACCWGGT	1 cut(s)	2278
Csp6I	GTAC	3 cut(s)	323, 894, 1555
CviQI	GTAC	3 cut(s)	323, 894, 1555
DdeI	CTNAG	5 cut(s)	483, 1307, 1602, 1900, 1938
EaeI	YGGCCR	2 cut(s)	855, 1709
Eam1104I	CTCTTC	1 cut(s)	307
EarI	CTCTTC	1 cut(s)	307
Ecl136II	GAGCTC	2 cut(s)	1569, 1937
Eco130I	CCWWGG	7 cut(s)	400, 705, 1190, 1631, 1858, 2125, 2158
Eco147I	AGGCCT	1 cut(s)	2177
Eco24I	GRGCYC	4 cut(s)	204, 615, 1571, 1939
Eco31I	GGTCTC	1 cut(s)	1823
Eco32I	GATATC	2 cut(s)	1210, 2297
Eco47I	GGWCC	5 cut(s)	180, 451, 1006, 1187, 1836
Eco53kI	GAGCTC	2 cut(s)	1569, 1937
Eco57I	CTGAAG	2 cut(s)	1455, 2168
Eco72I	CACGTG	1 cut(s)	2114
Eco81I	CCTNAGG	1 cut(s)	1602
Eco88I	CYCGRG	2 cut(s)	1446, 1820
EcoICRI	GAGCTC	2 cut(s)	1569, 1937
EcoNI	CCTNNNNNAGG	1 cut(s)	709
EcoO109I	RGGNCCY	1 cut(s)	1229
EcoRI	GAATTC	1 cut(s)	2348
EcoRV	GATATC	2 cut(s)	1210, 2297
EcoT14I	CCWWGG	7 cut(s)	400, 705, 1190, 1631, 1858, 2125, 2158
EcoT38I	GRGCYC	4 cut(s)	204, 615, 1571, 1939
ErhI	CCWWGG	7 cut(s)	400, 705, 1190, 1631, 1858, 2125, 2158
Esp3I	CGTCTC	1 cut(s)	735
FalI	AAGNNNNNCTT	2 cut(s)	1281, 1313
FaqI	GGGAC	2 cut(s)	18, 728
FauI	CCCGC	1 cut(s)	932
FauNDI	CATATG	1 cut(s)	319
Fnu4HI	GCNGC	5 cut(s)	255, 1410, 1443, 1522, 1944
FokI	GGATG	6 cut(s)	392, 824, 1256, 1478, 1511, 2054
FriOI	GRGCYC	4 cut(s)	204, 615, 1571, 1939
Fsp4HI	GCNGC	5 cut(s)	255, 1410, 1443, 1522, 1944
FspBI	CTAG	3 cut(s)	168, 1562, 1656
GlaI	GCGC	2 cut(s)	1525, 1615
GluI	GCNGC	5 cut(s)	255, 1410, 1443, 1522, 1944
GsaI	CCCAGC	2 cut(s)	628, 1455
GsuI	CTGGAG	3 cut(s)	513, 615, 1337
HaeII	RGCGCY	1 cut(s)	1617
HaeIII	GGCC	5 cut(s)	727, 857, 1231, 1711, 2177
HgaI	GACGC	2 cut(s)	15, 1959
HhaI	GCGC	2 cut(s)	1526, 1616
Hin1I	GRCGYC	1 cut(s)	744
Hin6I	GCGC	2 cut(s)	1524, 1614
HinP1I	GCGC	2 cut(s)	1524, 1614
HincII	GTYRAC	1 cut(s)	1990
HindII	GTYRAC	1 cut(s)	1990
HphI	GGTGA	4 cut(s)	739, 767, 977, 2227
Hpy166II	GTNNAC	8 cut(s)	137, 504, 959, 978, 1006, 1683, 1990, 2218
Hpy188I	TCNGA	9 cut(s)	1033, 1391, 1836, 1903, 1924, 2059, 2121, 2392, 2406
Hpy8I	GTNNAC	8 cut(s)	137, 504, 959, 978, 1006, 1683, 1990, 2218
Hpy99I	CGWCG	1 cut(s)	1953
HpyCH4III	ACNGT	6 cut(s)	112, 151, 242, 274, 1200, 1687
HpyCH4IV	ACGT	2 cut(s)	744, 2113
HpyF10VI	GCNNNNNNNGC	8 cut(s)	251, 610, 1312, 1448, 1583, 1808, 1943, 2072
HpyF3I	CTNAG	5 cut(s)	483, 1307, 1602, 1900, 1938
HpySE526I	ACGT	2 cut(s)	744, 2113
Hsp92I	GRCGYC	1 cut(s)	744
HspAI	GCGC	2 cut(s)	1524, 1614
LguI	GCTCTTC	1 cut(s)	307
LmnI	GCTCC	5 cut(s)	942, 1406, 1566, 1636, 2072
Lsp1109I	GCAGC	4 cut(s)	241, 1396, 1454, 1955
LweI	GCATC	8 cut(s)	308, 541, 611, 631, 1429, 1489, 1500, 1798
MabI	ACCWGGT	1 cut(s)	2278
MaeI	CTAG	3 cut(s)	168, 1562, 1656
MaeII	ACGT	2 cut(s)	744, 2113
MaeIII	GTNAC	4 cut(s)	442, 605, 745, 2282
MfeI	CAATTG	1 cut(s)	1395
MflI	RGATCY	6 cut(s)	1719, 1733, 1828, 2012, 2121, 2242
MhlI	GDGCHC	7 cut(s)	204, 247, 615, 961, 1571, 1939, 2157
MlsI	TGGCCA	2 cut(s)	857, 1711
MluNI	TGGCCA	2 cut(s)	857, 1711
MlyI	GAGTC	7 cut(s)	233, 290, 487, 710, 1736, 2048, 2254
MmeI	TCCRAC	1 cut(s)	2415
Mox20I	TGGCCA	2 cut(s)	857, 1711
MscI	TGGCCA	2 cut(s)	857, 1711
MseI	TTAA	6 cut(s)	305, 765, 1254, 1647, 1794, 2419
MslI	CAYNNNNRTG	1 cut(s)	1137
Msp20I	TGGCCA	2 cut(s)	857, 1711
MspCI	CTTAAG	1 cut(s)	1646
MunI	CAATTG	1 cut(s)	1395
MvnI	CGCG	1 cut(s)	1524
MwoI	GCNNNNNNNGC	8 cut(s)	251, 610, 1312, 1448, 1583, 1808, 1943, 2072
NcoI	CCATGG	2 cut(s)	400, 2125
NdeI	CATATG	1 cut(s)	319
NheI	GCTAGC	1 cut(s)	1655
NlaIV	GGNNCC	8 cut(s)	1168, 1197, 1721, 1830, 2047, 2074, 2106, 2383
NmuCI	GTSAC	2 cut(s)	745, 2282
NspI	RCATGY	4 cut(s)	422, 1018, 1625, 2167
PaeI	GCATGC	1 cut(s)	2167
PaeR7I	CTCGAG	2 cut(s)	1446, 1820
PagI	TCATGA	2 cut(s)	862, 1956
PceI	AGGCCT	1 cut(s)	2177
PciI	ACATGT	1 cut(s)	418
PciSI	GCTCTTC	1 cut(s)	307
PfeI	GAWTC	3 cut(s)	1028, 1864, 1919
PflMI	CCANNNNNTGG	2 cut(s)	734, 2114
PfoI	TCCNGGA	2 cut(s)	490, 1494
PkrI	GCNGC	5 cut(s)	256, 1411, 1444, 1523, 1945
PleI	GAGTC	7 cut(s)	232, 289, 486, 710, 1735, 2048, 2254
PmaCI	CACGTG	1 cut(s)	2114
PmlI	CACGTG	1 cut(s)	2114
PpsI	GAGTC	7 cut(s)	232, 289, 486, 710, 1735, 2048, 2254
Ppu21I	YACGTR	1 cut(s)	2114
PscI	ACATGT	1 cut(s)	418
PshBI	ATTAAT	1 cut(s)	305
Psp124BI	GAGCTC	2 cut(s)	1571, 1939
PspCI	CACGTG	1 cut(s)	2114
PspFI	CCCAGC	2 cut(s)	624, 1451
PspN4I	GGNNCC	8 cut(s)	1168, 1197, 1721, 1830, 2047, 2074, 2106, 2383
PspPI	GGNCC	6 cut(s)	180, 451, 1006, 1187, 1229, 1836
PspXI	VCTCGAGB	1 cut(s)	1446
PsrI	GAACNNNNNNTAC	2 cut(s)	2259, 2291
PstNI	CAGNNNCTG	2 cut(s)	624, 1736
PsuI	RGATCY	6 cut(s)	1719, 1733, 1828, 2012, 2121, 2242
RsaI	GTAC	3 cut(s)	324, 895, 1556
RsaNI	GTAC	3 cut(s)	323, 894, 1555
RseI	CAYNNNNRTG	1 cut(s)	1137
SacI	GAGCTC	2 cut(s)	1571, 1939
SapI	GCTCTTC	1 cut(s)	307
SaqAI	TTAA	6 cut(s)	305, 765, 1254, 1647, 1794, 2419
SatI	GCNGC	5 cut(s)	255, 1410, 1443, 1522, 1944
Sau96I	GGNCC	6 cut(s)	180, 451, 1006, 1187, 1229, 1836
ScaI	AGTACT	1 cut(s)	1556
SchI	GAGTC	7 cut(s)	233, 290, 487, 710, 1736, 2048, 2254
SduI	GDGCHC	7 cut(s)	204, 247, 615, 961, 1571, 1939, 2157
SexAI	ACCWGGT	1 cut(s)	2278
SfaNI	GCATC	8 cut(s)	308, 541, 611, 631, 1429, 1489, 1500, 1798
Sfr274I	CTCGAG	2 cut(s)	1446, 1820
SinI	GGWCC	5 cut(s)	180, 451, 1006, 1187, 1836
SlaI	CTCGAG	2 cut(s)	1446, 1820
SmiMI	CAYNNNNRTG	1 cut(s)	1137
SmlI	CTYRAG	4 cut(s)	718, 1446, 1646, 1820
SmoI	CTYRAG	4 cut(s)	718, 1446, 1646, 1820
SphI	GCATGC	1 cut(s)	2167
SseBI	AGGCCT	1 cut(s)	2177
SsiI	CCGC	4 cut(s)	66, 454, 939, 1522
SspI	AATATT	1 cut(s)	1749
SspMI	CTAG	3 cut(s)	168, 1562, 1656
SstI	GAGCTC	2 cut(s)	1571, 1939
StuI	AGGCCT	1 cut(s)	2177
StyI	CCWWGG	7 cut(s)	400, 705, 1190, 1631, 1858, 2125, 2158
TaaI	ACNGT	6 cut(s)	112, 151, 242, 274, 1200, 1687
TaiI	ACGT	2 cut(s)	747, 2116
TaqI	TCGA	5 cut(s)	1219, 1447, 1821, 2293, 2328
TaqII	GACCGA	1 cut(s)	1500
TatI	WGTACW	1 cut(s)	1554
TauI	GCSGC	1 cut(s)	1524
TfiI	GAWTC	3 cut(s)	1028, 1864, 1919
Tru1I	TTAA	6 cut(s)	305, 765, 1254, 1647, 1794, 2419
Tru9I	TTAA	6 cut(s)	305, 765, 1254, 1647, 1794, 2419
TscAI	CASTG	1 cut(s)	880
TseFI	GTSAC	2 cut(s)	745, 2282
TseI	GCWGC	4 cut(s)	254, 1409, 1442, 1943
Tsp45I	GTSAC	2 cut(s)	745, 2282
TspGWI	ACGGA	2 cut(s)	489, 2123
TspRI	CASTG	1 cut(s)	880
Van91I	CCANNNNNTGG	2 cut(s)	734, 2114
Vha464I	CTTAAG	1 cut(s)	1646
VneI	GTGCAC	1 cut(s)	957
VpaK11BI	GGWCC	5 cut(s)	180, 451, 1006, 1187, 1836
VspI	ATTAAT	1 cut(s)	305
XagI	CCTNNNNNAGG	1 cut(s)	709
XapI	RAATTY	9 cut(s)	57, 79, 372, 569, 582, 699, 1156, 1531, 2348
XceI	RCATGY	4 cut(s)	422, 1018, 1625, 2167
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1197
XhoI	CTCGAG	2 cut(s)	1446, 1820
XspI	CTAG	3 cut(s)	168, 1562, 1656
ZraI	GACGTC	1 cut(s)	745
ZrmI	AGTACT	1 cut(s)	1556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.