Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005265

Physical Location & Seq

Genomic Coordinates Forward (+)

32896360 .. 32901387

Gene Structure 5028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00108790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGGTGACGCGGCTGAGCATTGGCTACATGGGACGCTTCATGCTACGATCTATGAGGTTGATAAGCTGCACAGTAGCAGTGGAAATTTCCTCCGCAAGATCACTGGAAAACTTGAAGAGACTGTTGGTATTGGCAAAGGAATGAGTAAACTGTATGCAACTGTTGATCTGGAAAGGGCTAGGGTTGGCCGCACCAGAGTTGTAGGAGAACCCTCCCATCCCAAGTGGTTTGAGTCTTTCCACATTTATTGTGCTCATGTCGCTGCAAATGTTATATTCACTGTGAAGGAGTCGAATCCTATTGGAGCATCATTAATTGGTAGAGCGTATGTACCTGTTGAACAACTTTTTGAAGGGCAAGAAGTGGATACATGGGCTGAAATTTTGGATGAGAAAAAAGAACCTGTTCATGGCAGTCCCAAGATACATGTGAAGCTACAATTTTTTCATGTTTCAAAGGACCGCAGTTGGAGTCAAGGTATTAAGAGTCCTAAATTTCCTGGAGTTCCATTTACATTTTTCTCACAGAGACAAGGATGTAAGGTTACACTGTACCAAGATGCTCATGTCCCAGATAACTTTATTCCTAAGATTCCTCTTGCTGGAGGCAAGAATTATGAGCCCCACAGATGTTGGGAAGATATCTTTGATGCAATTACTAATGCAAAACACCTGATCTACATTACTGGGTGGTCAGTTTATACTGAAATTTCCTTGGTCAGGGACTCAAGGAGGTCAAAACCTGGTGGCGATATCACTATTGGTGAGTTACTTAAAAAGAAAGCAAGTGAAGGTGTCCGTGTTCTTATGCTTGTTTGGGATGACAGAACCTCTGTTCCTTTATTAAAAAAAGATGGAATGATGGCCACCCATGATGAGGCAACTGCGCAGTATTTCCAGAATACTGACGTGAACTGTGTCTTATGCCCCCGTAATCCAGATGGTGGTGGAAGCATTGTTCAGGGGGCACAAATCTCTACAATGTTCACTCATCATCAGAAGATTGTGGTTGTGGACAGTGAAATGCCAACTAGAGGATCACAGAGTAGAAGAATTGTGAGTTTTGTTGGGGGTATTGATCTGTGTGATGGAAGATATGATACCCAATTCCATTCACTTTTCAGGACATTGGATACTGCACATCATGACGATTTCCATCAGCCAAATTTTACTGGTGCATCGATTACAAAAGGTGGTCCAAGAGAACCTTGGCATGATATTCACTCTCGGCTGGAAGGGCCTATTGCTTGGGATGTGTTGTTTAACTTTGAGCAGAGATGGAGAAAGCAAGGTGGTAAAGATGTACTTGTTCAGCTCAGAGAGCTTGACAATGTTATTATTCCACCTTCTCCTGTTATGTTCCCAGATGACCATGATACATGGAATGTTCAGTTATTCAGATCAATTGATGGAGGAGCTGCTTTTGGCTTCCCGGATACGCCTGAAGATGCAGCCAGAGCTGGGCTTGTTAGTGGGAAGGATAACATCATTGATCGAAGCATTCAGGATGCTTATATCCATGCTATTCGCCGTGCAAAGAACTTCATATATATTGAGAATCAGTATTTTCTTGGTAGCTCTTTTGCCTGGGCAGCTGATGGTATAAAGCCCGAAGACATTGGTGCTTTGCATGTAATTCCTAGGGAGCTTTCGCTTAAGATTGCTGACAAGATTGCGAATGGGGAGAGGTTTACTGTCTATGTGGTTGTCCCAATGTGGCCAGAGGGAATCCCGGAGTCTGGACCAGTTCAGGCGATATTAGATTGGCAGAGGAGGACAATGCAAATGATGTACACAGATATTAAGCAGGCACTTGATAAAAACGGTGTTCAGGAGGATCCAAGGAACTATTTGACATTTTTCTGCCTTGGGAATCGGGAGGTGAAGAAGGATGGAGAATATGAACCCACTGAGCGACCAGAGGCAGATTCGGATTATATTAGAGCTCAGGAATCCCGACGCTTCATGATTTATGTTCATACCAAGATGATGATAGTTGACGACGAATACATCATCATTGGATCTGCCAACATCAACCAGAGATCAATGGATGGTGCCAGGGACTCTGAAATAGCCATGGGAGCCTACCAACCATATCATCTATCGGTCAGGGAGCCAGCACGTGGTCAGATTCATGGTTTCCGTATGGCATTGTGGTACGAGCACCTTGGCATGCTTGATGAGTCCTTCCTCCAGCCAGAAAGCATTGAATGTATCACAAAGGTTAACCAGATTGCTGACAAATACTGGGACCTCTATTCAAGTGAAACACTGGAACGTGATTTGCCGGGTCACCTGCTTCGCTATCCTGTTGGAGTTACCAGCGAAGGAGAAGTCACGGAGTTGCCCGGATTTGAATTCTTCCCTGACACCAAGGCTCGAGTGCTAGGTGCCAAATCAGACTATCTTCCTCCCATCATTACTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

809

Amino Acids

91.57

Weight (kDa)

5.85

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	46 - 127	4.3e-08	C2 domain
PLDc	PF00614	328 - 365	1.5e-06	Phospholipase D Active site motif
PLDc_2	PF13091	655 - 689	2.8e-06	PLD-like domain
PLDc	PF00614	657 - 682	3e-08	Phospholipase D Active site motif
PLD_C	PF12357	727 - 799	6.6e-30	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52570 AT1G52570 AT1G52570 AT3G15730
fragaria_vesca	FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920 FvH4_6g29920
malus_domestica	MD00G1090900.v1.1 MD17G1209200.v1.1
prunus_persica	Prupe.3G084800_v2.0.a1 Prupe.3G084800_v2.0.a1 Prupe.3G084800_v2.0.a1
pyrus_communis	pycom09g14080
rosa_chinensis	RchiOBHm_Chr2g0135901
rosa_laevigata	RLG00000019489
rosa_multiflora	Rmu_sc0001825.1_g000006 Rmu_sc0007848.1_g000004
rosa_roxburghii	Rroxscaffold_2G00108790
rosa_rugosa	Rorug02G0326700 Rorug02G0326700 Rorug02G0326700
rosa_samantha	Rh2AG378300 Rh2BG384800 Rh2CG364100 Rh2DG401200
rosa_wichuraiana	Rw2G030820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2304
Acc16I	TGCGCA	1 cut(s)	888
Acc36I	ACCTGC	1 cut(s)	2304
AccB1I	GGYRCC	2 cut(s)	2054, 2390
AccB7I	CCANNNNNTGG	2 cut(s)	944, 2123
AccII	CGCG	1 cut(s)	11
AciI	CCGC	4 cut(s)	11, 94, 190, 463
AclWI	GGATC	4 cut(s)	1045, 1832, 1845, 2029
AcoI	YGGCCR	3 cut(s)	187, 864, 1718
AcsI	RAATTY	6 cut(s)	85, 381, 494, 708, 1165, 2357
AcuI	CTGAAG	1 cut(s)	1464
AcvI	CACGTG	1 cut(s)	2123
AfaI	GTAC	5 cut(s)	333, 554, 1305, 1793, 2159
AfiI	CCNNNNNNNGG	9 cut(s)	410, 602, 720, 877, 944, 1034, 1461, 1739, 2123
AflII	CTTAAG	1 cut(s)	1655
AflIII	ACRYGT	1 cut(s)	427
AgsI	TTSAA	7 cut(s)	116, 341, 353, 456, 2210, 2262, 2357
AjiI	CACGTC	1 cut(s)	910
AjnI	CCWGG	4 cut(s)	499, 742, 1586, 2057
AjuI	GAANNNNNNNTTGG	2 cut(s)	108, 140
AloI	GAACNNNNNNTCC	4 cut(s)	820, 852, 942, 974
Alw21I	GWGCWC	3 cut(s)	256, 1948, 2166
Alw26I	GTCTC	2 cut(s)	113, 523
AlwI	GGATC	4 cut(s)	1045, 1832, 1845, 2029
Ama87I	CYCGRG	1 cut(s)	2379
AoxI	GGCC	4 cut(s)	187, 864, 1238, 1718
ApeKI	GCWGC	5 cut(s)	67, 263, 1418, 1451, 1592
ApoI	RAATTY	6 cut(s)	85, 381, 494, 708, 1165, 2357
ArsI	GACNNNNNNTTYG	2 cut(s)	2319, 2351
AseI	ATTAAT	1 cut(s)	314
Asp700I	GAANNNNTTC	1 cut(s)	405
AspA2I	CCTAGG	1 cut(s)	1640
AspLEI	GCGC	1 cut(s)	889
AspS9I	GGNCC	5 cut(s)	460, 1196, 1238, 1742, 2251
AsuC2I	CCSGG	4 cut(s)	1433, 1733, 2289, 2349
AsuHPI	GGTGA	4 cut(s)	17, 776, 1894, 2285
AvaI	CYCGRG	1 cut(s)	2379
AvaII	GGWCC	4 cut(s)	460, 1196, 1742, 2251
AvrII	CCTAGG	1 cut(s)	1640
BaeGI	GKGCMC	1 cut(s)	970
BalI	TGGCCA	2 cut(s)	866, 1720
BamHI	GGATCC	1 cut(s)	1837
BanI	GGYRCC	2 cut(s)	2054, 2390
BanII	GRGCYC	2 cut(s)	624, 1948
BarI	GAAGNNNNNNTAC	2 cut(s)	1084, 1116
BbrPI	CACGTG	1 cut(s)	2123
BbsI	GAAGAC	1 cut(s)	1620
Bbv12I	GWGCWC	3 cut(s)	256, 1948, 2166
BbvI	GCAGC	5 cut(s)	54, 250, 1405, 1463, 1604
BceAI	ACGGC	1 cut(s)	1515
BciT130I	CCWGG	4 cut(s)	501, 744, 1588, 2059
BciVI	GTATCC	3 cut(s)	361, 1126, 1429
BcnI	CCSGG	4 cut(s)	1433, 1733, 2289, 2349
BcoDI	GTCTC	2 cut(s)	113, 523
BfaI	CTAG	4 cut(s)	180, 1032, 1641, 2387
BfrI	CTTAAG	1 cut(s)	1655
BfuAI	ACCTGC	1 cut(s)	2304
BfuI	GTATCC	3 cut(s)	361, 1126, 1429
BisI	GCNGC	7 cut(s)	12, 68, 190, 264, 1419, 1452, 1593
BlnI	CCTAGG	1 cut(s)	1640
BlpI	GCTNAGC	1 cut(s)	15
BlsI	GCNGC	7 cut(s)	13, 69, 191, 265, 1420, 1453, 1594
Bme1390I	CCNGG	8 cut(s)	501, 744, 1433, 1588, 1733, 2059, 2289, 2349
Bme18I	GGWCC	4 cut(s)	460, 1196, 1742, 2251
BmeT110I	CYCGRG	1 cut(s)	2379
BmgBI	CACGTC	1 cut(s)	910
BmgT120I	GGNCC	5 cut(s)	460, 1196, 1238, 1742, 2251
BmiI	GGNNCC	6 cut(s)	1839, 2056, 2083, 2115, 2252, 2392
BmrFI	CCNGG	8 cut(s)	501, 744, 1433, 1588, 1733, 2059, 2289, 2349
BmrI	ACTGGG	2 cut(s)	696, 2257
BmsI	GCATC	6 cut(s)	317, 550, 640, 1187, 1438, 1498
BmuI	ACTGGG	2 cut(s)	696, 2257
BpiI	GAAGAC	1 cut(s)	1620
BpmI	CTGGAG	3 cut(s)	522, 624, 2177
Bpu10I	CCTNAGC	1 cut(s)	1947
Bpu1102I	GCTNAGC	1 cut(s)	15
BpuEI	CTTGAG	1 cut(s)	712
BpuMI	CCSGG	4 cut(s)	1433, 1733, 2289, 2349
Bsa29I	ATCGAT	1 cut(s)	1181
BsaAI	YACGTR	1 cut(s)	2123
BsaBI	GATNNNNATC	1 cut(s)	1155
BsaXI	ACNNNNNCTCC	2 cut(s)	463, 493
Bsc4I	CCNNNNNNNGG	9 cut(s)	410, 602, 720, 877, 944, 1034, 1461, 1739, 2123
Bse1I	ACTGG	6 cut(s)	109, 691, 1177, 1745, 2252, 2277
Bse8I	GATNNNNATC	1 cut(s)	1155
BseBI	CCWGG	4 cut(s)	501, 744, 1588, 2059
BseCI	ATCGAT	1 cut(s)	1181
BseGI	GGATG	8 cut(s)	217, 394, 542, 826, 1258, 1513, 1897, 2056
BseJI	GATNNNNATC	1 cut(s)	1155
BseLI	CCNNNNNNNGG	9 cut(s)	410, 602, 720, 877, 944, 1034, 1461, 1739, 2123
BseMII	CTCAG	4 cut(s)	6, 1330, 1902, 1961
BseNI	ACTGG	6 cut(s)	109, 691, 1177, 1745, 2252, 2277
BseRI	GAGGAG	2 cut(s)	1428, 1786
BseSI	GKGCMC	1 cut(s)	970
BseXI	GCAGC	5 cut(s)	54, 250, 1405, 1463, 1604
BseYI	CCCAGC	1 cut(s)	1460
BsgI	GTGCAG	2 cut(s)	53, 1122
Bsh1236I	CGCG	1 cut(s)	11
BshFI	GGCC	4 cut(s)	189, 866, 1240, 1720
BshNI	GGYRCC	2 cut(s)	2054, 2390
BshVI	ATCGAT	1 cut(s)	1181
BsiHKAI	GWGCWC	3 cut(s)	256, 1948, 2166
BsiHKCI	CYCGRG	1 cut(s)	2379
BsiSI	CCGG	4 cut(s)	1433, 1733, 2288, 2349
BslFI	GGGAC	7 cut(s)	46, 402, 554, 737, 1694, 2075, 2264
BslI	CCNNNNNNNGG	9 cut(s)	410, 602, 720, 877, 944, 1034, 1461, 1739, 2123
BsmAI	GTCTC	2 cut(s)	113, 523
BsmFI	GGGAC	7 cut(s)	46, 402, 554, 737, 1694, 2075, 2264
BsmI	GAATGC	1 cut(s)	1500
BsnI	GGCC	4 cut(s)	189, 866, 1240, 1720
BsoBI	CYCGRG	1 cut(s)	2379
Bsp1286I	GDGCHC	5 cut(s)	256, 624, 970, 1948, 2166
Bsp1407I	TGTACA	1 cut(s)	1791
Bsp1720I	GCTNAGC	1 cut(s)	15
Bsp19I	CCATGG	1 cut(s)	2076
BspACI	CCGC	4 cut(s)	11, 94, 190, 463
BspANI	GGCC	4 cut(s)	189, 866, 1240, 1720
BspCNI	CTCAG	4 cut(s)	7, 1329, 1903, 1960
BspDI	ATCGAT	1 cut(s)	1181
BspFNI	CGCG	1 cut(s)	11
BspHI	TCATGA	2 cut(s)	1144, 1965
BspLI	GGNNCC	6 cut(s)	1839, 2056, 2083, 2115, 2252, 2392
BspMI	ACCTGC	1 cut(s)	2304
BspPI	GGATC	4 cut(s)	1045, 1832, 1845, 2029
BspT107I	GGYRCC	2 cut(s)	2054, 2390
BspTI	CTTAAG	1 cut(s)	1655
BsrGI	TGTACA	1 cut(s)	1791
BsrI	ACTGG	6 cut(s)	109, 691, 1177, 1745, 2252, 2277
BssT1I	CCWWGG	8 cut(s)	714, 1208, 1640, 1841, 1867, 2076, 2167, 2373
Bst2UI	CCWGG	4 cut(s)	501, 744, 1588, 2059
Bst6I	CTCTTC	1 cut(s)	111
BstAFI	CTTAAG	1 cut(s)	1655
BstAUI	TGTACA	1 cut(s)	1791
BstBAI	YACGTR	1 cut(s)	2123
BstC8I	GCNNGC	3 cut(s)	1809, 2118, 2174
BstDEI	CTNAG	5 cut(s)	15, 588, 1316, 1911, 1947
BstDSI	CCRYGG	1 cut(s)	2076
BstEII	GGTNACC	1 cut(s)	2291
BstENI	CCTNNNNNAGG	1 cut(s)	718
BstF5I	GGATG	8 cut(s)	217, 394, 542, 826, 1258, 1513, 1897, 2056
BstFNI	CGCG	1 cut(s)	11
BstHHI	GCGC	1 cut(s)	889
BstMAI	GTCTC	2 cut(s)	113, 523
BstMWI	GCNNNNNNNGC	6 cut(s)	260, 1237, 1321, 1457, 1592, 2081
BstNI	CCWGG	4 cut(s)	501, 744, 1588, 2059
BstNSI	RCATGY	3 cut(s)	431, 1634, 2176
BstPI	GGTNACC	1 cut(s)	2291
BstSCI	CCNGG	8 cut(s)	499, 742, 1431, 1586, 1731, 2057, 2287, 2347
BstSLI	GKGCMC	1 cut(s)	970
BstUI	CGCG	1 cut(s)	11
BstV1I	GCAGC	5 cut(s)	54, 250, 1405, 1463, 1604
BstV2I	GAAGAC	1 cut(s)	1620
BstX2I	RGATCY	2 cut(s)	1837, 2021
BstYI	RGATCY	2 cut(s)	1837, 2021
Bsu15I	ATCGAT	1 cut(s)	1181
BsuI	GTATCC	3 cut(s)	361, 1126, 1429
BsuRI	GGCC	4 cut(s)	189, 866, 1240, 1720
BsuTUI	ATCGAT	1 cut(s)	1181
BtgI	CCRYGG	1 cut(s)	2076
BtrI	CACGTC	1 cut(s)	910
BtsCI	GGATG	8 cut(s)	217, 394, 542, 826, 1258, 1513, 1897, 2056
BtsI	GCAGTG	1 cut(s)	85
BtsIMutI	CAGTG	7 cut(s)	85, 102, 279, 548, 1024, 1908, 2270
BveI	ACCTGC	1 cut(s)	2304
Cac8I	GCNNGC	3 cut(s)	1809, 2118, 2174
CciI	TCATGA	2 cut(s)	1144, 1965
CfoI	GCGC	1 cut(s)	889
Cfr13I	GGNCC	5 cut(s)	460, 1196, 1238, 1742, 2251
ClaI	ATCGAT	1 cut(s)	1181
CseI	GACGC	3 cut(s)	17, 43, 1968
CsiI	ACCWGGT	1 cut(s)	742
Csp6I	GTAC	5 cut(s)	332, 553, 1304, 1792, 2158
CspCI	CAANNNNNGTGG	4 cut(s)	230, 265, 614, 649
CviQI	GTAC	5 cut(s)	332, 553, 1304, 1792, 2158
DdeI	CTNAG	5 cut(s)	15, 588, 1316, 1911, 1947
EaeI	YGGCCR	3 cut(s)	187, 864, 1718
Eam1104I	CTCTTC	1 cut(s)	111
EarI	CTCTTC	1 cut(s)	111
Ecl136II	GAGCTC	1 cut(s)	1946
Eco130I	CCWWGG	8 cut(s)	714, 1208, 1640, 1841, 1867, 2076, 2167, 2373
Eco24I	GRGCYC	2 cut(s)	624, 1948
Eco32I	GATATC	2 cut(s)	643, 754
Eco47I	GGWCC	4 cut(s)	460, 1196, 1742, 2251
Eco53kI	GAGCTC	1 cut(s)	1946
Eco57I	CTGAAG	1 cut(s)	1464
Eco72I	CACGTG	1 cut(s)	2123
Eco88I	CYCGRG	1 cut(s)	2379
Eco91I	GGTNACC	1 cut(s)	2291
EcoICRI	GAGCTC	1 cut(s)	1946
EcoNI	CCTNNNNNAGG	1 cut(s)	718
EcoO109I	RGGNCCY	2 cut(s)	1238, 2251
EcoO65I	GGTNACC	1 cut(s)	2291
EcoRI	GAATTC	1 cut(s)	2357
EcoRII	CCWGG	4 cut(s)	499, 742, 1586, 2057
EcoRV	GATATC	2 cut(s)	643, 754
EcoT14I	CCWWGG	8 cut(s)	714, 1208, 1640, 1841, 1867, 2076, 2167, 2373
EcoT38I	GRGCYC	2 cut(s)	624, 1948
ErhI	CCWWGG	8 cut(s)	714, 1208, 1640, 1841, 1867, 2076, 2167, 2373
FalI	AAGNNNNNCTT	4 cut(s)	1192, 1224, 1290, 1322
FaqI	GGGAC	7 cut(s)	46, 402, 554, 737, 1694, 2075, 2264
Fnu4HI	GCNGC	7 cut(s)	12, 68, 190, 264, 1419, 1452, 1593
FokI	GGATG	8 cut(s)	204, 401, 549, 833, 1265, 1520, 1904, 2063
FriOI	GRGCYC	2 cut(s)	624, 1948
Fsp4HI	GCNGC	7 cut(s)	12, 68, 190, 264, 1419, 1452, 1593
FspBI	CTAG	4 cut(s)	180, 1032, 1641, 2387
FspI	TGCGCA	1 cut(s)	888
GlaI	GCGC	1 cut(s)	888
GluI	GCNGC	7 cut(s)	12, 68, 190, 264, 1419, 1452, 1593
GsaI	CCCAGC	1 cut(s)	1464
GsuI	CTGGAG	3 cut(s)	522, 624, 2177
HaeIII	GGCC	4 cut(s)	189, 866, 1240, 1720
HapII	CCGG	4 cut(s)	1433, 1733, 2288, 2349
HgaI	GACGC	3 cut(s)	17, 43, 1968
HhaI	GCGC	1 cut(s)	889
Hin6I	GCGC	1 cut(s)	887
HinP1I	GCGC	1 cut(s)	887
HincII	GTYRAC	2 cut(s)	1999, 2227
HindII	GTYRAC	2 cut(s)	1999, 2227
HpaI	GTTAAC	1 cut(s)	2227
HpaII	CCGG	4 cut(s)	1433, 1733, 2288, 2349
HphI	GGTGA	4 cut(s)	17, 776, 1894, 2285
Hpy166II	GTNNAC	8 cut(s)	149, 913, 987, 1015, 1692, 1794, 1999, 2227
Hpy188I	TCNGA	7 cut(s)	999, 1319, 1400, 1933, 2068, 2130, 2401
Hpy8I	GTNNAC	8 cut(s)	149, 913, 987, 1015, 1692, 1794, 1999, 2227
Hpy99I	CGWCG	2 cut(s)	1962, 2006
HpyAV	CCTTC	9 cut(s)	280, 347, 785, 1229, 1356, 1471, 1882, 2197, 2321
HpyCH4IV	ACGT	3 cut(s)	909, 2122, 2278
HpyF10VI	GCNNNNNNNGC	6 cut(s)	260, 1237, 1321, 1457, 1592, 2081
HpyF3I	CTNAG	5 cut(s)	15, 588, 1316, 1911, 1947
HpySE526I	ACGT	3 cut(s)	909, 2122, 2278
HspAI	GCGC	1 cut(s)	887
KspAI	GTTAAC	1 cut(s)	2227
LmnI	GCTCC	5 cut(s)	305, 1415, 1645, 2081, 2113
Lsp1109I	GCAGC	5 cut(s)	54, 250, 1405, 1463, 1604
LweI	GCATC	6 cut(s)	317, 550, 640, 1187, 1438, 1498
MabI	ACCWGGT	1 cut(s)	742
MaeI	CTAG	4 cut(s)	180, 1032, 1641, 2387
MaeII	ACGT	3 cut(s)	909, 2122, 2278
MaeIII	GTNAC	6 cut(s)	5, 544, 768, 2291, 2317, 2335
MfeI	CAATTG	1 cut(s)	1404
MflI	RGATCY	2 cut(s)	1837, 2021
MhlI	GDGCHC	5 cut(s)	256, 624, 970, 1948, 2166
MlsI	TGGCCA	2 cut(s)	866, 1720
MluNI	TGGCCA	2 cut(s)	866, 1720
MlyI	GAGTC	8 cut(s)	242, 299, 481, 496, 719, 1745, 2057, 2192
MmeI	TCCRAC	2 cut(s)	449, 2293
Mox20I	TGGCCA	2 cut(s)	866, 1720
MroXI	GAANNNNTTC	1 cut(s)	405
MscI	TGGCCA	2 cut(s)	866, 1720
MseI	TTAA	8 cut(s)	314, 483, 774, 845, 1263, 1656, 1803, 2226
Msp20I	TGGCCA	2 cut(s)	866, 1720
MspA1I	CMGCKG	1 cut(s)	1595
MspCI	CTTAAG	1 cut(s)	1655
MspI	CCGG	4 cut(s)	1433, 1733, 2288, 2349
MspR9I	CCNGG	8 cut(s)	501, 744, 1433, 1588, 1733, 2059, 2289, 2349
MunI	CAATTG	1 cut(s)	1404
Mva1269I	GAATGC	1 cut(s)	1500
MvaI	CCWGG	4 cut(s)	501, 744, 1588, 2059
MvnI	CGCG	1 cut(s)	11
MwoI	GCNNNNNNNGC	6 cut(s)	260, 1237, 1321, 1457, 1592, 2081
NciI	CCSGG	4 cut(s)	1433, 1733, 2289, 2349
NcoI	CCATGG	1 cut(s)	2076
NlaIV	GGNNCC	6 cut(s)	1839, 2056, 2083, 2115, 2252, 2392
NmeAIII	GCCGAG	1 cut(s)	1207
NmuCI	GTSAC	3 cut(s)	5, 2291, 2335
NsbI	TGCGCA	1 cut(s)	888
NspI	RCATGY	3 cut(s)	431, 1634, 2176
PaeI	GCATGC	1 cut(s)	2176
PaeR7I	CTCGAG	1 cut(s)	2379
PagI	TCATGA	2 cut(s)	1144, 1965
PaqCI	CACCTGC	1 cut(s)	2304
PciI	ACATGT	1 cut(s)	427
PctI	GAATGC	1 cut(s)	1500
PdmI	GAANNNNTTC	1 cut(s)	405
PfeI	GAWTC	8 cut(s)	295, 592, 1558, 1728, 1873, 1928, 1952, 2131
PflMI	CCANNNNNTGG	2 cut(s)	944, 2123
PfoI	TCCNGGA	3 cut(s)	499, 1431, 1731
PkrI	GCNGC	7 cut(s)	13, 69, 191, 265, 1420, 1453, 1594
PleI	GAGTC	8 cut(s)	241, 298, 480, 495, 719, 1744, 2057, 2191
PmaCI	CACGTG	1 cut(s)	2123
PmlI	CACGTG	1 cut(s)	2123
PpsI	GAGTC	8 cut(s)	241, 298, 480, 495, 719, 1744, 2057, 2191
Ppu21I	YACGTR	1 cut(s)	2123
PpuMI	RGGWCCY	1 cut(s)	2251
PscI	ACATGT	1 cut(s)	427
PshBI	ATTAAT	1 cut(s)	314
Psp124BI	GAGCTC	1 cut(s)	1948
Psp5II	RGGWCCY	1 cut(s)	2251
Psp6I	CCWGG	4 cut(s)	499, 742, 1586, 2057
PspCI	CACGTG	1 cut(s)	2123
PspEI	GGTNACC	1 cut(s)	2291
PspFI	CCCAGC	1 cut(s)	1460
PspGI	CCWGG	4 cut(s)	499, 742, 1586, 2057
PspN4I	GGNNCC	6 cut(s)	1839, 2056, 2083, 2115, 2252, 2392
PspPI	GGNCC	5 cut(s)	460, 1196, 1238, 1742, 2251
PspPPI	RGGWCCY	1 cut(s)	2251
PspXI	VCTCGAGB	1 cut(s)	2379
PsuI	RGATCY	2 cut(s)	1837, 2021
PvuII	CAGCTG	1 cut(s)	1595
RsaI	GTAC	5 cut(s)	333, 554, 1305, 1793, 2159
RsaNI	GTAC	5 cut(s)	332, 553, 1304, 1792, 2158
SacI	GAGCTC	1 cut(s)	1948
SaqAI	TTAA	8 cut(s)	314, 483, 774, 845, 1263, 1656, 1803, 2226
SatI	GCNGC	7 cut(s)	12, 68, 190, 264, 1419, 1452, 1593
Sau96I	GGNCC	5 cut(s)	460, 1196, 1238, 1742, 2251
SchI	GAGTC	8 cut(s)	242, 299, 481, 496, 719, 1745, 2057, 2192
ScrFI	CCNGG	8 cut(s)	501, 744, 1433, 1588, 1733, 2059, 2289, 2349
SduI	GDGCHC	5 cut(s)	256, 624, 970, 1948, 2166
SexAI	ACCWGGT	1 cut(s)	742
SfaNI	GCATC	6 cut(s)	317, 550, 640, 1187, 1438, 1498
Sfr274I	CTCGAG	1 cut(s)	2379
SinI	GGWCC	4 cut(s)	460, 1196, 1742, 2251
SlaI	CTCGAG	1 cut(s)	2379
SmlI	CTYRAG	3 cut(s)	727, 1655, 2379
SmoI	CTYRAG	3 cut(s)	727, 1655, 2379
SphI	GCATGC	1 cut(s)	2176
SsiI	CCGC	4 cut(s)	11, 94, 190, 463
SspMI	CTAG	4 cut(s)	180, 1032, 1641, 2387
SstI	GAGCTC	1 cut(s)	1948
StyD4I	CCNGG	8 cut(s)	499, 742, 1431, 1586, 1731, 2057, 2287, 2347
StyI	CCWWGG	8 cut(s)	714, 1208, 1640, 1841, 1867, 2076, 2167, 2373
TaiI	ACGT	3 cut(s)	912, 2125, 2281
TaqI	TCGA	4 cut(s)	293, 1181, 1495, 2380
TaqII	GACCGA	1 cut(s)	2095
TatI	WGTACW	2 cut(s)	1303, 1791
TauI	GCSGC	2 cut(s)	14, 192
TfiI	GAWTC	8 cut(s)	295, 592, 1558, 1728, 1873, 1928, 1952, 2131
Tru1I	TTAA	8 cut(s)	314, 483, 774, 845, 1263, 1656, 1803, 2226
Tru9I	TTAA	8 cut(s)	314, 483, 774, 845, 1263, 1656, 1803, 2226
TscAI	CASTG	7 cut(s)	85, 109, 286, 555, 1024, 1915, 2277
TseFI	GTSAC	3 cut(s)	5, 2291, 2335
TseI	GCWGC	5 cut(s)	67, 263, 1418, 1451, 1592
Tsp45I	GTSAC	3 cut(s)	5, 2291, 2335
TspDTI	ATGAA	8 cut(s)	29, 398, 437, 1534, 1917, 1954, 1967, 2123
TspGWI	ACGGA	3 cut(s)	788, 2132, 2354
TspRI	CASTG	7 cut(s)	85, 109, 286, 555, 1024, 1915, 2277
Van91I	CCANNNNNTGG	2 cut(s)	944, 2123
Vha464I	CTTAAG	1 cut(s)	1655
VpaK11BI	GGWCC	4 cut(s)	460, 1196, 1742, 2251
VspI	ATTAAT	1 cut(s)	314
XagI	CCTNNNNNAGG	1 cut(s)	718
XapI	RAATTY	6 cut(s)	85, 381, 494, 708, 1165, 2357
XceI	RCATGY	3 cut(s)	431, 1634, 2176
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1206
XhoI	CTCGAG	1 cut(s)	2379
XmaJI	CCTAGG	1 cut(s)	1640
XmnI	GAANNNNTTC	1 cut(s)	405
XspI	CTAG	4 cut(s)	180, 1032, 1641, 2387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.