AT1G53040

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003383
Physical Location & Seq
Reverse (-)
19764245 .. 19767507
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53040.6

Sequence Viewer

Length: 1623 bp
ATGCTCACTTCTAATTCTAAAGAGAAGGAAAGGTCTCTCTCCTTCTTATGTTGCTGGTACTTAGGTCGTCGACGAGTTGCTATGCTTCTACTTCTCAGCCTTGCTTTTGTTGTCTTTGTGTTGGGTTCCTACACTATCAACAAAGAGAGCAATAGTCCAAATATTCATCAGAGTATTGAGACGATGGATTTTGGAAGCAACCAAACACCGATAAGTAGAGAATTGACTTCTTTCTATACAAAGGAGTCTGATAATGATCATGTTAGAGATCCTTTTTTGTGGAATGGCATTGGAGGAAGTGATGTAGACGTCAACCATCCTCCTCCATTCTTACCTTCATGGCATCACCATCCGTGTGATAGTTTTTCTTTTCCTCCTCCTCCACCACCTGGAATGAGAAGGCCTGGTCCACGCCCTTGTCCGGTATGCTACCTACCTCCAGAAGAGGCCTTAGCACATATGCCAAAGTATCCATTTGAATCTCCCTTGCTCAAGAATTTGACATATATACGTGAAGAGAGTCCTGTAAAACCTGAAGAAAGTGAAGGAGGCTCTGAATTCGGAGGTTATCCTTCTCTCGAGCATAGGACTAACTCTTTTGACATAAAAGAGTCCATGACAGTGCATTGCGGGTTTATTAAAGGAACAAAACCGGGCCACCAAACTGGATTTGACATCGATGAGGATATCCTTCATGAGTTGGACCAGTCCCATGATGTGATTGTCGCGTCAGCGATATTTGGAAAGTATGACATAATTCAAGAACCGGTGAACATCAGTGAAATGGCGAGGAAGAACATCCCATTCTACATGTTTGTTGATGAAGAAACACATTTATACCTTAAGAACACGTCGAGTTATACAGACGATAACAAGAGAGTGGGATTGTGGAGGATCATTGTTGTACATAATGTCCCTTATACCGATGCACGGCGTAATGGAAAGGTTCCGAAGCTTTTATTGCATAGATTATTCCCAAATGTCCGGTACTCTATATGGGTTGATGCAAAGCTCCAGCTTGTTGTAGATCCATATCAAATCCTTGAAAGGTTCTTGTGGAGAACGAATTCTAGTTTTGCAATCTCGAGACATTACCGACGTTTTGATGTGTTTGTAGAGGCTGAAGCGAATAAGGCCGCAAGAAAGTACGACAATGCCTCAATAGATTACCAAGTAGAGTTCTACAAGAAGGAAGGTTTAACACCTTATACGGAGGCTAAGCTTCCAATAACAAGTGATGTCCCTGAAGGTTGTACCATCATAAGAGAACACATACCGATCACAAACCTTTTCACATGTGTATGGTTCAACGAGGTAGATCGGTTTACTTCAAGAGATCAGCTAAGCTTTGCAATTGCGAGAGACAAAATAAGAGAGAAAGTTGATTGGAGCATCAATATGTTCTTGGACTGTGAAAGACGCAACTTTGTGAAACAGGTATATCATAGAGACGTTTTGCTGACCATGAAACCTCCTCGAGCTTCTTCTAGAGTTCTTCCCGAGCCACTGGTTTTACCACGTGGAAGATTAGCCGGTGGTAGAGCCACGCCGGGTAAGAAGACTCCGGGACAACGCGGGAAAAGGCGTCACCGGAAAGTTTCAGCCGGTGGCAGAAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

540

Amino Acids

62.17

Weight (kDa)

8.76

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 167 - 482 3.7e-151 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
AccB7I CCANNNNNTGG 1 cut(s) 389
AccI GTMKAC 2 cut(s) 70, 306
AccII CGCG 2 cut(s) 728, 1575
AciI CCGC 3 cut(s) 630, 1137, 1575
AclWI GGATC 3 cut(s) 263, 902, 1022
AcsI RAATTY 3 cut(s) 496, 557, 1066
AcuI CTGAAG 3 cut(s) 555, 1143, 1266
AcvI CACGTG 1 cut(s) 1520
AcyI GRCGYC 2 cut(s) 309, 1585
AfaI GTAC 5 cut(s) 59, 906, 989, 1148, 1255
AfiI CCNNNNNNNGG 3 cut(s) 389, 421, 930
AflII CTTAAG 1 cut(s) 842
AflIII ACRYGT 4 cut(s) 810, 849, 1295, 1617
AgeI ACCGGT 1 cut(s) 766
AgsI TTSAA 5 cut(s) 479, 761, 1046, 1309, 1332
AjiI CACGTC 1 cut(s) 852
AjnI CCWGG 2 cut(s) 388, 403
AluBI AGCT 7 cut(s) 955, 1012, 1018, 1222, 1342, 1347, 1481
AluI AGCT 7 cut(s) 955, 1012, 1018, 1222, 1342, 1347, 1481
Alw26I GTCTC 5 cut(s) 39, 173, 1081, 1356, 1443
AlwI GGATC 3 cut(s) 263, 902, 1022
Ama87I CYCGRG 4 cut(s) 578, 1084, 1476, 1499
AoxI GGCC 4 cut(s) 401, 447, 655, 1134
ApoI RAATTY 3 cut(s) 496, 557, 1066
AsiGI ACCGGT 1 cut(s) 766
Asp700I GAANNNNTTC 1 cut(s) 1066
AspS9I GGNCC 3 cut(s) 407, 655, 703
AsuC2I CCSGG 3 cut(s) 654, 1551, 1566
AsuHPI GGTGA 3 cut(s) 338, 781, 1580
AvaI CYCGRG 4 cut(s) 578, 1084, 1476, 1499
AvaII GGWCC 2 cut(s) 407, 703
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BbrPI CACGTG 1 cut(s) 1520
BbsI GAAGAC 1 cut(s) 1565
BccI CCATC 4 cut(s) 178, 324, 357, 1265
BceAI ACGGC 1 cut(s) 947
BciT130I CCWGG 2 cut(s) 390, 405
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 3 cut(s) 654, 1551, 1566
BcoDI GTCTC 5 cut(s) 39, 173, 1081, 1356, 1443
BfaI CTAG 2 cut(s) 1071, 1488
BfrI CTTAAG 1 cut(s) 842
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 1 cut(s) 1137
BlpI GCTNAGC 2 cut(s) 1218, 1343
BlsI GCNGC 1 cut(s) 1138
Bme1390I CCNGG 5 cut(s) 390, 405, 654, 1551, 1566
Bme18I GGWCC 2 cut(s) 407, 703
BmeT110I CYCGRG 4 cut(s) 578, 1084, 1476, 1499
BmgBI CACGTC 1 cut(s) 852
BmgT120I GGNCC 3 cut(s) 407, 655, 703
BmiI GGNNCC 2 cut(s) 127, 948
BmrFI CCNGG 5 cut(s) 390, 405, 654, 1551, 1566
BmsI GCATC 4 cut(s) 352, 916, 994, 1401
BpiI GAAGAC 1 cut(s) 1565
BpmI CTGGAG 2 cut(s) 423, 998
Bpu10I CCTNAGC 1 cut(s) 451
Bpu1102I GCTNAGC 2 cut(s) 1218, 1343
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 3 cut(s) 654, 1551, 1566
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 2 cut(s) 512, 1520
BsaBI GATNNNNATC 2 cut(s) 255, 1032
BsaHI GRCGYC 2 cut(s) 309, 1585
BsaI GGTCTC 1 cut(s) 39
BsaWI WCCGGW 4 cut(s) 421, 766, 984, 1590
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 421, 930
Bse118I RCCGGY 3 cut(s) 766, 1532, 1604
Bse1I ACTGG 3 cut(s) 670, 706, 1512
Bse3DI GCAATG 1 cut(s) 625
Bse8I GATNNNNATC 2 cut(s) 255, 1032
BseBI CCWGG 2 cut(s) 390, 405
BseCI ATCGAT 1 cut(s) 678
BseGI GGATG 3 cut(s) 316, 349, 798
BseJI GATNNNNATC 2 cut(s) 255, 1032
BseLI CCNNNNNNNGG 3 cut(s) 389, 421, 930
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 1 cut(s) 109
BseNI ACTGG 3 cut(s) 670, 706, 1512
BseRI GAGGAG 4 cut(s) 312, 366, 369, 1464
Bsh1236I CGCG 2 cut(s) 728, 1575
BshFI GGCC 4 cut(s) 403, 449, 657, 1136
BshTI ACCGGT 1 cut(s) 766
BshVI ATCGAT 1 cut(s) 678
BsiHKCI CYCGRG 4 cut(s) 578, 1084, 1476, 1499
BsiSI CCGG 9 cut(s) 422, 653, 767, 985, 1533, 1550, 1565, 1591, 1605
BslFI GGGAC 4 cut(s) 694, 899, 1226, 1581
BslI CCNNNNNNNGG 3 cut(s) 389, 421, 930
BsmAI GTCTC 5 cut(s) 39, 173, 1081, 1356, 1443
BsmBI CGTCTC 2 cut(s) 173, 1443
BsmFI GGGAC 4 cut(s) 694, 899, 1226, 1581
BsnI GGCC 4 cut(s) 403, 449, 657, 1136
Bso31I GGTCTC 1 cut(s) 39
BsoBI CYCGRG 4 cut(s) 578, 1084, 1476, 1499
Bsp1407I TGTACA 1 cut(s) 904
Bsp143I GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
Bsp1720I GCTNAGC 2 cut(s) 1218, 1343
BspACI CCGC 3 cut(s) 630, 1137, 1575
BspANI GGCC 4 cut(s) 403, 449, 657, 1136
BspCNI CTCAG 1 cut(s) 108
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 2 cut(s) 728, 1575
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 127, 948
BspPI GGATC 3 cut(s) 263, 902, 1022
BspTI CTTAAG 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 625
BsrFI RCCGGY 3 cut(s) 766, 1532, 1604
BsrGI TGTACA 1 cut(s) 904
BsrI ACTGG 3 cut(s) 670, 706, 1512
BssAI RCCGGY 3 cut(s) 766, 1532, 1604
BssMI GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
BssNI GRCGYC 2 cut(s) 309, 1585
Bst2UI CCWGG 2 cut(s) 390, 405
Bst4CI ACNGT 2 cut(s) 622, 1412
Bst6I CTCTTC 2 cut(s) 438, 510
BstACI GRCGYC 2 cut(s) 309, 1585
BstAFI CTTAAG 1 cut(s) 842
BstAUI TGTACA 1 cut(s) 904
BstBAI YACGTR 2 cut(s) 512, 1520
BstDEI CTNAG 5 cut(s) 61, 95, 451, 1218, 1343
BstF5I GGATG 3 cut(s) 316, 349, 798
BstFNI CGCG 2 cut(s) 728, 1575
BstKTI GATC 7 cut(s) 259, 271, 897, 1030, 1281, 1321, 1339
BstMAI GTCTC 5 cut(s) 39, 173, 1081, 1356, 1443
BstMBI GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
BstMWI GCNNNNNNNGC 2 cut(s) 961, 1133
BstNI CCWGG 2 cut(s) 390, 405
BstNSI RCATGY 3 cut(s) 814, 1299, 1621
BstSCI CCNGG 5 cut(s) 388, 403, 652, 1549, 1564
BstUI CGCG 2 cut(s) 728, 1575
BstV2I GAAGAC 1 cut(s) 1565
BstX2I RGATCY 2 cut(s) 268, 1027
BstXI CCANNNNNNTGG 1 cut(s) 665
BstYI RGATCY 2 cut(s) 268, 1027
Bsu15I ATCGAT 1 cut(s) 678
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 4 cut(s) 403, 449, 657, 1136
BsuTUI ATCGAT 1 cut(s) 678
BtrI CACGTC 1 cut(s) 852
BtsCI GGATG 3 cut(s) 316, 349, 798
BtsIMutI CAGTG 3 cut(s) 627, 784, 1505
CciI TCATGA 1 cut(s) 694
Cfr10I RCCGGY 3 cut(s) 766, 1532, 1604
Cfr13I GGNCC 3 cut(s) 407, 655, 703
ClaI ATCGAT 1 cut(s) 678
CseI GACGC 3 cut(s) 717, 1428, 1574
Csp6I GTAC 5 cut(s) 58, 905, 988, 1147, 1254
CspAI ACCGGT 1 cut(s) 766
CviAII CATG 9 cut(s) 260, 339, 616, 695, 713, 811, 1296, 1465, 1618
CviQI GTAC 5 cut(s) 58, 905, 988, 1147, 1254
DdeI CTNAG 5 cut(s) 61, 95, 451, 1218, 1343
DpnI GATC 7 cut(s) 258, 270, 896, 1029, 1280, 1320, 1338
DpnII GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
Eam1104I CTCTTC 2 cut(s) 438, 510
EarI CTCTTC 2 cut(s) 438, 510
Eco147I AGGCCT 2 cut(s) 403, 449
Eco31I GGTCTC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 407, 703
Eco57I CTGAAG 3 cut(s) 555, 1143, 1266
Eco72I CACGTG 1 cut(s) 1520
Eco88I CYCGRG 4 cut(s) 578, 1084, 1476, 1499
EcoRI GAATTC 2 cut(s) 557, 1066
EcoRII CCWGG 2 cut(s) 388, 403
EcoRV GATATC 1 cut(s) 688
Esp3I CGTCTC 2 cut(s) 173, 1443
FaeI CATG 9 cut(s) 263, 342, 619, 698, 716, 814, 1299, 1468, 1621
FaqI GGGAC 4 cut(s) 694, 899, 1226, 1581
FatI CATG 9 cut(s) 259, 338, 615, 694, 712, 810, 1295, 1464, 1617
FauI CCCGC 2 cut(s) 623, 1568
FauNDI CATATG 1 cut(s) 459
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 2 cut(s) 70, 306
Fnu4HI GCNGC 1 cut(s) 1137
FokI GGATG 3 cut(s) 303, 336, 785
Fsp4HI GCNGC 1 cut(s) 1137
FspBI CTAG 2 cut(s) 1071, 1488
GluI GCNGC 1 cut(s) 1137
GsuI CTGGAG 2 cut(s) 423, 998
HaeIII GGCC 4 cut(s) 403, 449, 657, 1136
HapII CCGG 9 cut(s) 422, 653, 767, 985, 1533, 1550, 1565, 1591, 1605
HgaI GACGC 3 cut(s) 717, 1428, 1574
Hin1I GRCGYC 2 cut(s) 309, 1585
Hin1II CATG 9 cut(s) 263, 342, 619, 698, 716, 814, 1299, 1468, 1621
HincII GTYRAC 2 cut(s) 71, 313
HindII GTYRAC 2 cut(s) 71, 313
HindIII AAGCTT 3 cut(s) 953, 1220, 1345
HinfI GANTC 5 cut(s) 245, 479, 520, 611, 1561
HpaII CCGG 9 cut(s) 422, 653, 767, 985, 1533, 1550, 1565, 1591, 1605
HphI GGTGA 3 cut(s) 338, 781, 1580
Hpy166II GTNNAC 6 cut(s) 71, 307, 313, 410, 772, 1326
Hpy188I TCNGA 5 cut(s) 171, 250, 556, 563, 951
Hpy8I GTNNAC 6 cut(s) 71, 307, 313, 410, 772, 1326
Hpy99I CGWCG 4 cut(s) 72, 75, 856, 1101
HpyCH4III ACNGT 2 cut(s) 622, 1412
HpyCH4IV ACGT 6 cut(s) 309, 511, 851, 1099, 1452, 1519
HpyCH4V TGCA 6 cut(s) 625, 929, 964, 1007, 1079, 1352
HpyF10VI GCNNNNNNNGC 2 cut(s) 961, 1133
HpyF3I CTNAG 5 cut(s) 61, 95, 451, 1218, 1343
HpySE526I ACGT 6 cut(s) 309, 511, 851, 1099, 1452, 1519
Hsp92I GRCGYC 2 cut(s) 309, 1585
Hsp92II CATG 9 cut(s) 263, 342, 619, 698, 716, 814, 1299, 1468, 1621
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
LmnI GCTCC 2 cut(s) 1017, 1389
LweI GCATC 4 cut(s) 352, 916, 994, 1401
MaeI CTAG 2 cut(s) 1071, 1488
MaeII ACGT 6 cut(s) 309, 511, 851, 1099, 1452, 1519
MaeIII GTNAC 1 cut(s) 1586
MalI GATC 7 cut(s) 258, 270, 896, 1029, 1280, 1320, 1338
MboI GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
MboII GAAGA 9 cut(s) 455, 527, 548, 805, 836, 1476, 1487, 1536, 1570
MfeI CAATTG 1 cut(s) 1353
MflI RGATCY 2 cut(s) 268, 1027
MluCI AATT 7 cut(s) 13, 221, 496, 557, 756, 1066, 1353
MlyI GAGTC 4 cut(s) 254, 529, 620, 1555
MmeI TCCRAC 1 cut(s) 681
MroXI GAANNNNTTC 1 cut(s) 1066
MseI TTAA 3 cut(s) 639, 843, 1199
MslI CAYNNNNRTG 4 cut(s) 354, 620, 1300, 1397
MspCI CTTAAG 1 cut(s) 842
MspI CCGG 9 cut(s) 422, 653, 767, 985, 1533, 1550, 1565, 1591, 1605
MspR9I CCNGG 5 cut(s) 390, 405, 654, 1551, 1566
MunI CAATTG 1 cut(s) 1353
MvaI CCWGG 2 cut(s) 390, 405
MvnI CGCG 2 cut(s) 728, 1575
MwoI GCNNNNNNNGC 2 cut(s) 961, 1133
NciI CCSGG 3 cut(s) 654, 1551, 1566
NdeI CATATG 1 cut(s) 459
NdeII GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
NlaIII CATG 9 cut(s) 263, 342, 619, 698, 716, 814, 1299, 1468, 1621
NlaIV GGNNCC 2 cut(s) 127, 948
NmuCI GTSAC 1 cut(s) 1586
NspI RCATGY 3 cut(s) 814, 1299, 1621
PaeR7I CTCGAG 3 cut(s) 578, 1084, 1476
PagI TCATGA 1 cut(s) 694
PceI AGGCCT 2 cut(s) 403, 449
PciI ACATGT 3 cut(s) 810, 1295, 1617
PdmI GAANNNNTTC 1 cut(s) 1066
PfeI GAWTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 1564
PinAI ACCGGT 1 cut(s) 766
PkrI GCNGC 1 cut(s) 1138
PleI GAGTC 4 cut(s) 253, 528, 619, 1555
PmaCI CACGTG 1 cut(s) 1520
PmlI CACGTG 1 cut(s) 1520
PpsI GAGTC 4 cut(s) 253, 528, 619, 1555
Ppu21I YACGTR 2 cut(s) 512, 1520
PscI ACATGT 3 cut(s) 810, 1295, 1617
Psp6I CCWGG 2 cut(s) 388, 403
PspCI CACGTG 1 cut(s) 1520
PspGI CCWGG 2 cut(s) 388, 403
PspN4I GGNNCC 2 cut(s) 127, 948
PspPI GGNCC 3 cut(s) 407, 655, 703
PspXI VCTCGAGB 1 cut(s) 1476
PsuI RGATCY 2 cut(s) 268, 1027
RsaI GTAC 5 cut(s) 59, 906, 989, 1148, 1255
RsaNI GTAC 5 cut(s) 58, 905, 988, 1147, 1254
RseI CAYNNNNRTG 4 cut(s) 354, 620, 1300, 1397
SalI GTCGAC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 639, 843, 1199
SatI GCNGC 1 cut(s) 1137
Sau3AI GATC 7 cut(s) 256, 268, 894, 1027, 1278, 1318, 1336
Sau96I GGNCC 3 cut(s) 407, 655, 703
SchI GAGTC 4 cut(s) 254, 529, 620, 1555
ScrFI CCNGG 5 cut(s) 390, 405, 654, 1551, 1566
SfaNI GCATC 4 cut(s) 352, 916, 994, 1401
Sfr274I CTCGAG 3 cut(s) 578, 1084, 1476
SgrDI CGTCGACG 1 cut(s) 69
SinI GGWCC 2 cut(s) 407, 703
SlaI CTCGAG 3 cut(s) 578, 1084, 1476
SmiMI CAYNNNNRTG 4 cut(s) 354, 620, 1300, 1397
SmlI CTYRAG 5 cut(s) 491, 578, 842, 1084, 1476
SmoI CTYRAG 5 cut(s) 491, 578, 842, 1084, 1476
Sse9I AATT 7 cut(s) 13, 221, 496, 557, 756, 1066, 1353
SseBI AGGCCT 2 cut(s) 403, 449
SsiI CCGC 3 cut(s) 630, 1137, 1575
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 1071, 1488
StuI AGGCCT 2 cut(s) 403, 449
StyD4I CCNGG 5 cut(s) 388, 403, 652, 1549, 1564
TaaI ACNGT 2 cut(s) 622, 1412
TaiI ACGT 6 cut(s) 312, 514, 854, 1102, 1455, 1522
TaqI TCGA 6 cut(s) 70, 579, 678, 854, 1085, 1477
TasI AATT 7 cut(s) 13, 221, 496, 557, 756, 1066, 1353
TatI WGTACW 1 cut(s) 904
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 3 cut(s) 639, 843, 1199
Tru9I TTAA 3 cut(s) 639, 843, 1199
TscAI CASTG 3 cut(s) 627, 784, 1512
TseFI GTSAC 1 cut(s) 1586
Tsp45I GTSAC 1 cut(s) 1586
TspDTI ATGAA 5 cut(s) 155, 327, 683, 837, 1481
TspGWI ACGGA 2 cut(s) 342, 1226
TspRI CASTG 3 cut(s) 627, 784, 1512
Van91I CCANNNNNTGG 1 cut(s) 389
Vha464I CTTAAG 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 407, 703
XapI RAATTY 3 cut(s) 496, 557, 1066
XbaI TCTAGA 1 cut(s) 1487
XceI RCATGY 3 cut(s) 814, 1299, 1621
XhoI CTCGAG 3 cut(s) 578, 1084, 1476
XmiI GTMKAC 2 cut(s) 70, 306
XmnI GAANNNNTTC 1 cut(s) 1066
XspI CTAG 2 cut(s) 1071, 1488
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.