Rorug05G0141300

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003383
Physical Location & Seq
Forward (+)
12911137 .. 12911778
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0141300.1

Sequence Viewer

Length: 642 bp
ATGTTGAAACCTAAGCTCAGCTCTCCCCTGGCCCTAAAGCCAAGGACCATGGTCACTGTGTGCTCAGCCACCCCAAACCCAGAGAAAATATCCACTGCTTCAGCAATGAAGACTTTCTCTGCTGCACTAGCACTCTCCTCCATCATCTTGGGCGCAGCTCCCATCCCAGCCATGGCTGACATCTCAGGCCTAACCCCATGCAAAGACTCCAAGCAGTTCGCCAAGCGCGAGAAGCAGTCGATCAAGAAGCTTGAGTCCTCATTGAAGCTGTACGCACCGGACAGCGCACCTGCCTTGGCCATCAATGCAACAATAGAGAAGACCAAGAGAAGGTTTGACAACTACGGCAAGCAGGGTTTGCTCTGTGGGTCTGATGGGCTTCCCCATTTGATTGTGAGCGGTGACCAGAGGCACTGGGGTGAGTTCATCACTCCCGGGATTCTGTTCCTTTACATTGCTGGGTGGATCGGGTGGGTTGGAAGGAGCTACTTGATTGCCATTAGGGATGATAAGAAGCCAACACAGAAGGAGATCATCATTGATGTGCCTTTGGCTACTGGCTTGGTTTTCAGAGGCTTCAGTTGGCCCATTGCTGCTTACAGAGAGTTGTTGAATGGTGAACTCATTGCCAAGGACGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

213

Amino Acids

23.14

Weight (kDa)

9.58

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaF PF02507 50 - 212 1e-80 Photosystem I reaction centre subunit III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 399
AccII CGCG 1 cut(s) 228
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 473
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 84, 562
AdeI CACNNNGTG 1 cut(s) 60
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 172, 330
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 3 cut(s) 7, 265, 613
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 1 cut(s) 27
AleI CACNNNNGTG 1 cut(s) 417
AluBI AGCT 6 cut(s) 16, 21, 158, 250, 268, 486
AluI AGCT 6 cut(s) 16, 21, 158, 250, 268, 486
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 1 cut(s) 473
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 4 cut(s) 30, 187, 297, 584
ApeKI GCWGC 3 cut(s) 122, 155, 593
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 3 cut(s) 155, 228, 287
AspS9I GGNCC 3 cut(s) 31, 45, 585
AsuC2I CCSGG 2 cut(s) 435, 436
AsuHPI GGTGA 3 cut(s) 413, 431, 629
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 45
BalI TGGCCA 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 116, 326
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 109, 167, 580
BccI CCATC 4 cut(s) 149, 170, 308, 368
BceAI ACGGC 1 cut(s) 361
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 2 cut(s) 435, 436
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 3 cut(s) 123, 156, 594
BlpI GCTNAGC 2 cut(s) 17, 64
BlsI GCNGC 3 cut(s) 124, 157, 595
Bme1390I CCNGG 3 cut(s) 29, 435, 436
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 434
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 3 cut(s) 31, 45, 585
BmrFI CCNGG 3 cut(s) 29, 435, 436
BmrI ACTGGG 1 cut(s) 424
BmuI ACTGGG 1 cut(s) 424
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 116, 326
Bpu10I CCTNAGC 1 cut(s) 12
Bpu1102I GCTNAGC 2 cut(s) 17, 64
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 2 cut(s) 435, 436
BsaJI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 330
Bse1I ACTGG 2 cut(s) 419, 562
Bse3DI GCAATG 4 cut(s) 111, 453, 588, 624
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
BseGI GGATG 2 cut(s) 162, 511
BseLI CCNNNNNNNGG 2 cut(s) 172, 330
BseMI GCAATG 4 cut(s) 111, 453, 588, 624
BseMII CTCAG 3 cut(s) 31, 78, 198
BseNI ACTGG 2 cut(s) 419, 562
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 3 cut(s) 109, 167, 580
BseYI CCCAGC 2 cut(s) 166, 458
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 4 cut(s) 32, 189, 299, 586
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 2 cut(s) 278, 435
BslI CCNNNNNNNGG 2 cut(s) 172, 330
BsnI GGCC 4 cut(s) 32, 189, 299, 586
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 240, 465, 531
Bsp1720I GCTNAGC 2 cut(s) 17, 64
Bsp19I CCATGG 2 cut(s) 48, 171
BspACI CCGC 1 cut(s) 399
BspANI GGCC 4 cut(s) 32, 189, 299, 586
BspCNI CTCAG 3 cut(s) 30, 77, 197
BspFNI CGCG 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 399
BsrDI GCAATG 4 cut(s) 111, 453, 588, 624
BsrI ACTGG 2 cut(s) 419, 562
BssECI CCNNGG 7 cut(s) 27, 41, 48, 171, 294, 434, 630
BssMI GATC 3 cut(s) 240, 465, 531
BssT1I CCWWGG 5 cut(s) 41, 48, 171, 294, 630
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 12, 17, 64, 184
BstDSI CCRYGG 2 cut(s) 48, 171
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 2 cut(s) 162, 511
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 3 cut(s) 155, 228, 287
BstKTI GATC 3 cut(s) 243, 468, 534
BstMBI GATC 3 cut(s) 240, 465, 531
BstMWI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
BstNI CCWGG 1 cut(s) 29
BstPAI GACNNNNGTC 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 3 cut(s) 27, 433, 434
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 109, 167, 580
BstV2I GAAGAC 2 cut(s) 116, 326
BstXI CCANNNNNNTGG 1 cut(s) 148
BsuRI GGCC 4 cut(s) 32, 189, 299, 586
BtgI CCRYGG 2 cut(s) 48, 171
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 2 cut(s) 162, 511
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 3 cut(s) 54, 93, 412
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 350
CfoI GCGC 3 cut(s) 155, 228, 287
Cfr13I GGNCC 3 cut(s) 31, 45, 585
Cfr9I CCCGGG 1 cut(s) 434
Csp6I GTAC 1 cut(s) 271
CviAII CATG 3 cut(s) 49, 172, 198
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 4 cut(s) 12, 17, 64, 184
DpnI GATC 3 cut(s) 242, 467, 533
DpnII GATC 3 cut(s) 240, 465, 531
DraIII CACNNNGTG 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 297
Eco130I CCWWGG 5 cut(s) 41, 48, 171, 294, 630
Eco147I AGGCCT 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 45
Eco57I CTGAAG 2 cut(s) 84, 562
Eco88I CYCGRG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 5 cut(s) 41, 48, 171, 294, 630
ErhI CCWWGG 5 cut(s) 41, 48, 171, 294, 630
FaeI CATG 3 cut(s) 52, 175, 201
FaiI YATR 3 cut(s) 50, 173, 199
FatI CATG 3 cut(s) 48, 171, 197
Fnu4HI GCNGC 3 cut(s) 123, 156, 594
FokI GGATG 2 cut(s) 149, 518
Fsp4HI GCNGC 3 cut(s) 123, 156, 594
FspBI CTAG 1 cut(s) 128
GlaI GCGC 3 cut(s) 154, 227, 286
GluI GCNGC 3 cut(s) 123, 156, 594
GsaI CCCAGC 2 cut(s) 170, 462
HaeIII GGCC 4 cut(s) 32, 189, 299, 586
HapII CCGG 2 cut(s) 278, 435
HhaI GCGC 3 cut(s) 155, 228, 287
Hin1II CATG 3 cut(s) 52, 175, 201
Hin6I GCGC 3 cut(s) 153, 226, 285
HinP1I GCGC 3 cut(s) 153, 226, 285
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 3 cut(s) 206, 254, 439
HpaII CCGG 2 cut(s) 278, 435
HphI GGTGA 3 cut(s) 413, 431, 629
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 2 cut(s) 373, 572
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 3 cut(s) 324, 474, 520
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 636
HpyCH4V TGCA 3 cut(s) 125, 201, 308
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
HpyF3I CTNAG 4 cut(s) 12, 17, 64, 184
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 3 cut(s) 52, 175, 201
HspAI GCGC 3 cut(s) 153, 226, 285
Kzo9I GATC 3 cut(s) 240, 465, 531
LmnI GCTCC 2 cut(s) 163, 483
Lsp1109I GCAGC 3 cut(s) 109, 167, 580
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 2 cut(s) 52, 401
MalI GATC 3 cut(s) 242, 467, 533
MbiI CCGCTC 1 cut(s) 399
MboI GATC 3 cut(s) 240, 465, 531
MboII GAAGA 2 cut(s) 121, 331
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 2 cut(s) 200, 263
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 4 cut(s) 148, 268, 402, 566
Mox20I TGGCCA 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 113
MscI TGGCCA 1 cut(s) 299
MslI CAYNNNNRTG 2 cut(s) 417, 542
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 2 cut(s) 278, 435
MspR9I CCNGG 3 cut(s) 29, 435, 436
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 4 cut(s) 128, 232, 305, 358
NciI CCSGG 2 cut(s) 435, 436
NcoI CCATGG 2 cut(s) 48, 171
NdeII GATC 3 cut(s) 240, 465, 531
NlaIII CATG 3 cut(s) 52, 175, 201
NmuCI GTSAC 2 cut(s) 52, 401
OliI CACNNNNGTG 1 cut(s) 417
PaqCI CACCTGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 3 cut(s) 124, 157, 595
PleI GAGTC 2 cut(s) 200, 262
PpsI GAGTC 2 cut(s) 200, 262
PshAI GACNNNNGTC 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 27
PspEI GGTNACC 1 cut(s) 401
PspFI CCCAGC 2 cut(s) 166, 458
PspGI CCWGG 1 cut(s) 27
PspPI GGNCC 3 cut(s) 31, 45, 585
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 2 cut(s) 417, 542
SatI GCNGC 3 cut(s) 123, 156, 594
Sau3AI GATC 3 cut(s) 240, 465, 531
Sau96I GGNCC 3 cut(s) 31, 45, 585
SchI GAGTC 2 cut(s) 200, 263
ScrFI CCNGG 3 cut(s) 29, 435, 436
SduI GDGCHC 1 cut(s) 65
SinI GGWCC 1 cut(s) 45
SmaI CCCGGG 1 cut(s) 436
SmiMI CAYNNNNRTG 2 cut(s) 417, 542
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 1 cut(s) 399
SspMI CTAG 1 cut(s) 128
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 27, 433, 434
StyI CCWWGG 5 cut(s) 41, 48, 171, 294, 630
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 239
TfiI GAWTC 1 cut(s) 439
TscAI CASTG 3 cut(s) 61, 100, 419
TseFI GTSAC 2 cut(s) 52, 401
TseI GCWGC 3 cut(s) 122, 155, 593
Tsp45I GTSAC 2 cut(s) 52, 401
TspDTI ATGAA 2 cut(s) 122, 415
TspMI CCCGGG 1 cut(s) 434
TspRI CASTG 3 cut(s) 61, 100, 419
VpaK11BI GGWCC 1 cut(s) 45
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmaI CCCGGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.