FvH4_3g19940

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003383
Physical Location & Seq
Forward (+)
13097406 .. 13103217
5812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19940.t4

Sequence Viewer

Length: 1380 bp
ATGAAAGGAAAAGATGCAGATAGAGCCTTACTTTCTCCTCCTCCTCCTCCCCATCTCCATACTGAGTCATTTACTGTTGTTCATGGAAAATCCCCTCTGTTGGCCCATCTATGTGAAAATTTTGCATTTCCTCCTCCTCCTCCTCCATCTGGTCGAAGACGAAGTGGACCTCGCCCGTGTCCAGTATGTTATCTCCCTGTGGAGCAGGCTATAGCTAGTATGCCAACTTCTCCTTCAGAATCACCTGTACTACATAATTTGACATATGTTTATGATGAAAATCCGACTAGAATTGAACCACATGGAGGTTCTGAATTTGGTGGATTCCCAACTCTGAGGGAGAGAAATGAATCATATGATATAAAAGAATCCATGACGGTTCACTGTGGATTTGTTAAAGGCAATAAACCTGGGGACCAGTCTGGATTTGATGTTGATGAAGTGGACCTTATGGAGTTGGAGACGTCCCATCAGATAATTGTTGCCTCAGCTGTCTTTGGAAACTATGACATAATACAGCAGCCTACAAACATTAGTGAGTTTTCGAGGGAAAGTGTACCGTTCTACATGTTTATTGATGAAGAGACGGAAGCTTATATGAAGAATTCCAGTGTCCTGGGAAACAATAGGAAAGTAGGATTATGGAGAGTTATTGTTGTCCGTAACATTCCTTACACTGATGCAAGACGCAATGGGAAGATTCCAAAGCTTCTATTGCATAGGCTTTTCCCCAATGTCAGATATTCTATATGGATTGATGGAAAGCTTCGGCTTGTTGTGGATCCTTATCAGATCCTTGAGAGGTTCTTGTGGCGTCAGAATGCAAATTTTGCAATCTCAAAGCACTATAGACGGTTTGATGTTTTTGAAGAGGCTGAAGCTAACAGAGCTGCTGGGAAGTACGATAATTCATCCATTAATGAGCAGGTTGACTTCTATATCAAGGAGGGTTTACAACCTTATTCTGAGGCTAAGCTTCCAATAACAAGTGATGTTCCTGAAGGTTGTGTGATCATAAAGGAACACATTCCCATCACAAATTTATTCACCTGTAATTGGTTCAATGAAGTTGATCGTTTTACCTCCAGGGATCAGTTAAGCTTTTCCACAGTGAGGGATAAAATTATGGCAAAAGTTGACTGGAGCGTTAATATGTTTTTGGATTGTGAAAGGCGCAACTTTGTCATTCAGGCATACCACAGAGAGTTGCTGGAGCAGATGCCTCCTCCCCCTCCTCCGAGGGTTGTGATTCATCGTCCACCAGCTTTGCCTCATGGTATCACATCTGTAAAGACTCCACCTGGGAAGAAGCGTGGACGTGGAGATAAAAAGCGTCATCGGAAGGTTGTTACTGTTAGTAGAGACAACAGGCAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

460

Amino Acids

52.55

Weight (kDa)

8.68

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 191 - 501 3e-146 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 916
AclWI GGATC 4 cut(s) 776, 787, 789, 1098
AcsI RAATTY 5 cut(s) 118, 314, 604, 826, 1039
AcuI CTGAAG 3 cut(s) 219, 897, 1020
AcyI GRCGYC 2 cut(s) 464, 814
AfaI GTAC 3 cut(s) 249, 558, 902
AfiI CCNNNNNNNGG 5 cut(s) 100, 149, 305, 1056, 1113
AflIII ACRYGT 1 cut(s) 567
AgsI TTSAA 3 cut(s) 296, 869, 1063
AjiI CACGTC 1 cut(s) 1319
AjnI CCWGG 4 cut(s) 409, 615, 1085, 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
Alw26I GTCTC 3 cut(s) 455, 578, 1356
AlwI GGATC 4 cut(s) 776, 787, 789, 1098
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 2 cut(s) 520, 890
ApoI RAATTY 5 cut(s) 118, 314, 604, 826, 1039
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 1176
AspS9I GGNCC 4 cut(s) 103, 167, 415, 445
AsuHPI GGTGA 2 cut(s) 234, 1039
AvaII GGWCC 3 cut(s) 167, 415, 445
BamHI GGATCC 1 cut(s) 781
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
BbsI GAAGAC 1 cut(s) 163
BbvCI CCTCAGC 1 cut(s) 487
BbvI GCAGC 2 cut(s) 532, 877
BccI CCATC 6 cut(s) 60, 114, 154, 477, 752, 1040
BciT130I CCWGG 4 cut(s) 411, 617, 1087, 1302
BclI TGATCA 1 cut(s) 1011
BcoDI GTCTC 3 cut(s) 455, 578, 1356
BfaI CTAG 2 cut(s) 216, 288
BfmI CTRYAG 2 cut(s) 210, 847
BfuAI ACCTGC 1 cut(s) 916
BisI GCNGC 2 cut(s) 521, 891
BlpI GCTNAGC 1 cut(s) 972
BlsI GCNGC 2 cut(s) 522, 892
Bme1390I CCNGG 4 cut(s) 411, 617, 1087, 1302
Bme18I GGWCC 3 cut(s) 167, 415, 445
BmgBI CACGTC 1 cut(s) 1319
BmgT120I GGNCC 4 cut(s) 103, 167, 415, 445
BmiI GGNNCC 2 cut(s) 416, 783
BmrFI CCNGG 4 cut(s) 411, 617, 1087, 1302
BmsI GCATC 3 cut(s) 4, 670, 1209
BpiI GAAGAC 1 cut(s) 163
BpmI CTGGAG 3 cut(s) 1069, 1162, 1232
Bpu10I CCTNAGC 1 cut(s) 487
Bpu1102I GCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 818
BsaBI GATNNNNATC 2 cut(s) 279, 786
BsaHI GRCGYC 2 cut(s) 464, 814
BsaJI CCNNGG 5 cut(s) 410, 616, 1086, 1238, 1301
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 149, 305, 1056, 1113
Bse1I ACTGG 4 cut(s) 182, 418, 609, 1145
Bse3DI GCAATG 1 cut(s) 697
Bse8I GATNNNNATC 2 cut(s) 279, 786
BseBI CCWGG 4 cut(s) 411, 617, 1087, 1302
BseDI CCNNGG 5 cut(s) 410, 616, 1086, 1238, 1301
BseGI GGATG 1 cut(s) 911
BseJI GATNNNNATC 2 cut(s) 279, 786
BseLI CCNNNNNNNGG 5 cut(s) 100, 149, 305, 1056, 1113
BseMI GCAATG 1 cut(s) 697
BseMII CTCAG 4 cut(s) 54, 326, 501, 957
BseNI ACTGG 4 cut(s) 182, 418, 609, 1145
BseXI GCAGC 2 cut(s) 532, 877
BseYI CCCAGC 1 cut(s) 893
BshFI GGCC 1 cut(s) 104
BslFI GGGAC 2 cut(s) 428, 451
BslI CCNNNNNNNGG 5 cut(s) 100, 149, 305, 1056, 1113
BsmAI GTCTC 3 cut(s) 455, 578, 1356
BsmBI CGTCTC 2 cut(s) 455, 578
BsmFI GGGAC 2 cut(s) 428, 451
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 1 cut(s) 104
Bsp143I GATC 5 cut(s) 781, 792, 1011, 1072, 1090
Bsp1720I GCTNAGC 1 cut(s) 972
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 4 cut(s) 55, 327, 500, 958
BspLI GGNNCC 2 cut(s) 416, 783
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 4 cut(s) 776, 787, 789, 1098
BsrDI GCAATG 1 cut(s) 697
BsrI ACTGG 4 cut(s) 182, 418, 609, 1145
BssECI CCNNGG 5 cut(s) 410, 616, 1086, 1238, 1301
BssMI GATC 5 cut(s) 781, 792, 1011, 1072, 1090
BssNI GRCGYC 2 cut(s) 464, 814
Bst2UI CCWGG 4 cut(s) 411, 617, 1087, 1302
Bst4CI ACNGT 7 cut(s) 76, 379, 386, 561, 855, 1111, 1354
Bst6I CTCTTC 2 cut(s) 576, 864
BstACI GRCGYC 2 cut(s) 464, 814
BstAPI GCANNNNNTGC 1 cut(s) 830
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 5 cut(s) 63, 335, 487, 966, 972
BstF5I GGATG 1 cut(s) 911
BstHHI GCGC 1 cut(s) 1176
BstKTI GATC 5 cut(s) 784, 795, 1014, 1075, 1093
BstMAI GTCTC 3 cut(s) 455, 578, 1356
BstMBI GATC 5 cut(s) 781, 792, 1011, 1072, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 23, 715, 830, 887
BstNI CCWGG 4 cut(s) 411, 617, 1087, 1302
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 4 cut(s) 409, 615, 1085, 1300
BstSFI CTRYAG 2 cut(s) 210, 847
BstV1I GCAGC 2 cut(s) 532, 877
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 2 cut(s) 781, 792
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 2 cut(s) 781, 792
BsuRI GGCC 1 cut(s) 104
BtrI CACGTC 1 cut(s) 1319
BtsCI GGATG 1 cut(s) 911
BtsIMutI CAGTG 4 cut(s) 382, 616, 675, 1116
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 1 cut(s) 207
CfoI GCGC 1 cut(s) 1176
Cfr13I GGNCC 4 cut(s) 103, 167, 415, 445
CseI GACGC 3 cut(s) 696, 803, 1322
Csp6I GTAC 3 cut(s) 248, 557, 901
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
CviAII CATG 5 cut(s) 83, 302, 373, 568, 1274
CviQI GTAC 3 cut(s) 248, 557, 901
DdeI CTNAG 5 cut(s) 63, 335, 487, 966, 972
DpnI GATC 5 cut(s) 783, 794, 1013, 1074, 1092
DpnII GATC 5 cut(s) 781, 792, 1011, 1072, 1090
Eam1104I CTCTTC 2 cut(s) 576, 864
EarI CTCTTC 2 cut(s) 576, 864
Eco47I GGWCC 3 cut(s) 167, 415, 445
Eco57I CTGAAG 3 cut(s) 219, 897, 1020
EcoRI GAATTC 1 cut(s) 604
EcoRII CCWGG 4 cut(s) 409, 615, 1085, 1300
Esp3I CGTCTC 2 cut(s) 455, 578
FaeI CATG 5 cut(s) 86, 305, 376, 571, 1277
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FaqI GGGAC 2 cut(s) 428, 451
FatI CATG 5 cut(s) 82, 301, 372, 567, 1273
FauNDI CATATG 2 cut(s) 265, 355
FbaI TGATCA 1 cut(s) 1011
Fnu4HI GCNGC 2 cut(s) 521, 891
FokI GGATG 1 cut(s) 898
Fsp4HI GCNGC 2 cut(s) 521, 891
FspBI CTAG 2 cut(s) 216, 288
GlaI GCGC 1 cut(s) 1175
GluI GCNGC 2 cut(s) 521, 891
GsaI CCCAGC 1 cut(s) 897
GsuI CTGGAG 3 cut(s) 1069, 1162, 1232
HaeIII GGCC 1 cut(s) 104
HgaI GACGC 3 cut(s) 696, 803, 1322
HhaI GCGC 1 cut(s) 1176
Hin1I GRCGYC 2 cut(s) 464, 814
Hin1II CATG 5 cut(s) 86, 305, 376, 571, 1277
Hin6I GCGC 1 cut(s) 1174
HinP1I GCGC 1 cut(s) 1174
HincII GTYRAC 2 cut(s) 931, 1138
HindII GTYRAC 2 cut(s) 931, 1138
HindIII AAGCTT 5 cut(s) 591, 707, 764, 974, 1099
HinfI GANTC 8 cut(s) 65, 239, 324, 350, 368, 700, 1249, 1294
HphI GGTGA 2 cut(s) 234, 1039
Hpy166II GTNNAC 9 cut(s) 167, 382, 445, 557, 931, 953, 1138, 1259, 1316
Hpy188III TCNNGA 2 cut(s) 423, 998
Hpy8I GTNNAC 9 cut(s) 167, 382, 445, 557, 931, 953, 1138, 1259, 1316
HpyAV CCTTC 3 cut(s) 243, 995, 1336
HpyCH4III ACNGT 7 cut(s) 76, 379, 386, 561, 855, 1111, 1354
HpyCH4IV ACGT 2 cut(s) 464, 1318
HpyCH4V TGCA 6 cut(s) 17, 125, 683, 718, 824, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 715, 830, 887
HpyF3I CTNAG 5 cut(s) 63, 335, 487, 966, 972
HpySE526I ACGT 2 cut(s) 464, 1318
Hsp92I GRCGYC 2 cut(s) 464, 814
Hsp92II CATG 5 cut(s) 86, 305, 376, 571, 1277
HspAI GCGC 1 cut(s) 1174
Ksp22I TGATCA 1 cut(s) 1011
Kzo9I GATC 5 cut(s) 781, 792, 1011, 1072, 1090
LmnI GCTCC 3 cut(s) 202, 1143, 1213
Lsp1109I GCAGC 2 cut(s) 532, 877
LweI GCATC 3 cut(s) 4, 670, 1209
MaeI CTAG 2 cut(s) 216, 288
MaeII ACGT 2 cut(s) 464, 1318
MaeIII GTNAC 2 cut(s) 662, 1348
MalI GATC 5 cut(s) 783, 794, 1013, 1074, 1092
MboI GATC 5 cut(s) 781, 792, 1011, 1072, 1090
MboII GAAGA 6 cut(s) 168, 593, 613, 709, 881, 1318
MflI RGATCY 2 cut(s) 781, 792
MlyI GAGTC 2 cut(s) 74, 1288
MmeI TCCRAC 2 cut(s) 308, 438
MroXI GAANNNNTTC 1 cut(s) 1026
MseI TTAA 4 cut(s) 396, 918, 1097, 1149
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 491
MspR9I CCNGG 4 cut(s) 411, 617, 1087, 1302
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 4 cut(s) 411, 617, 1087, 1302
MwoI GCNNNNNNNGC 4 cut(s) 23, 715, 830, 887
NdeI CATATG 2 cut(s) 265, 355
NdeII GATC 5 cut(s) 781, 792, 1011, 1072, 1090
NlaIII CATG 5 cut(s) 86, 305, 376, 571, 1277
NlaIV GGNNCC 2 cut(s) 416, 783
NspI RCATGY 1 cut(s) 571
PciI ACATGT 1 cut(s) 567
PctI GAATGC 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 6 cut(s) 239, 324, 350, 368, 700, 1249
PkrI GCNGC 2 cut(s) 522, 892
PleI GAGTC 2 cut(s) 73, 1288
PpsI GAGTC 2 cut(s) 73, 1288
PscI ACATGT 1 cut(s) 567
PshBI ATTAAT 1 cut(s) 918
Psp6I CCWGG 4 cut(s) 409, 615, 1085, 1300
PspFI CCCAGC 1 cut(s) 893
PspGI CCWGG 4 cut(s) 409, 615, 1085, 1300
PspN4I GGNNCC 2 cut(s) 416, 783
PspPI GGNCC 4 cut(s) 103, 167, 415, 445
PsuI RGATCY 2 cut(s) 781, 792
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 3 cut(s) 249, 558, 902
RsaNI GTAC 3 cut(s) 248, 557, 901
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 4 cut(s) 396, 918, 1097, 1149
SatI GCNGC 2 cut(s) 521, 891
Sau3AI GATC 5 cut(s) 781, 792, 1011, 1072, 1090
Sau96I GGNCC 4 cut(s) 103, 167, 415, 445
SchI GAGTC 2 cut(s) 74, 1288
ScrFI CCNGG 4 cut(s) 411, 617, 1087, 1302
SfaNI GCATC 3 cut(s) 4, 670, 1209
SfcI CTRYAG 2 cut(s) 210, 847
SinI GGWCC 3 cut(s) 167, 415, 445
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
SspMI CTAG 2 cut(s) 216, 288
StyD4I CCNGG 4 cut(s) 409, 615, 1085, 1300
TaaI ACNGT 7 cut(s) 76, 379, 386, 561, 855, 1111, 1354
TaiI ACGT 2 cut(s) 467, 1321
TaqI TCGA 2 cut(s) 154, 545
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 6 cut(s) 239, 324, 350, 368, 700, 1249
Tru1I TTAA 4 cut(s) 396, 918, 1097, 1149
Tru9I TTAA 4 cut(s) 396, 918, 1097, 1149
TscAI CASTG 4 cut(s) 389, 616, 682, 1116
TseI GCWGC 2 cut(s) 520, 890
TspGWI ACGGA 2 cut(s) 602, 650
TspRI CASTG 4 cut(s) 389, 616, 682, 1116
VpaK11BI GGWCC 3 cut(s) 167, 415, 445
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 5 cut(s) 118, 314, 604, 826, 1039
XceI RCATGY 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 2 cut(s) 216, 288
ZraI GACGTC 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.