Rw5G021200

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003383
Physical Location & Seq
Forward (+)
28316521 .. 28321533
5013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021200.1

Sequence Viewer

Length: 1767 bp
ATGACTGGAGGGTCATTAGGTCTACGTACCGGAAGCTATGGATCACTACAACTACAGCAGATACAGAATGGTTTTCCACCTATTCTGCAGACAACCCCTCCTCCTCTTGCGCGAAAGCCTTCCAAGGTGCTTCTTGCAAGTAGGGAGAAGGAAAGGGTTCTGCCCTTTGTCTGTAGATTTTTGGGCCGAAAAAAGGTCATAATGCTTCTTTTGGTTGTCCTTGCTCTTTTGGTTTTCGTATCCAATTCCTTCAATGTAAATAAAGCAATCATGTCTTTTACTCAACATAGTCGAAATGCCATATCTTACTGCATGGTGATATCAAGTAGTTCCGTAATTTTCGCAGGAATGGAAGATAAACATGACATTAAAATTACTTCTACACAGAATACCATGAAAGGAAAAGATGCAAATAGAGCCCTACTTACTCCTCCTCCCCATCTTCATACTGAGTCATTTACTGCTGTTCATGGAAATTCCCCTCCATTGGCCCATCAATGTGAAAATTTTGCATTTCCTCCTCCTCCTCCTTCATCTGGTCGAAAACGAAGTGGTCCTCGCCCGTGTCCAGTATGTTATCTCCCTGTGGAGCAGGCTATAGCTAGTATGCCAAGTTCTTCTTCAGAATCACCTGTACTACATAATTTGACATATGTTTATGATGAAAATCCCACTAGAACTGAACGACATGGAGGTTCTGAATTTGGTGGATTTCCAACTCTGAGACAGAGAAATGATTCATATGATATAAAAGAATCCATGACGGTCCACTGTGGATTTGTGAAAGGCAATAAACCTGGTGACCAGTCTGGATTTGATGTTGATGAAGCTGACCTTATGGAGTTGGAGACATCCCATGAGATAATTGTTGCATCAGCTGTCTTTGGAAACTATGACATAATACAGCAGCCAACAAACATTAGTGGGTTTTCGAGGAAAAATGTTCCATTCTACATGTTTATTGATGAAGAGACTGAAGCTTATATGAAGAATTCCAGTGTCCTGGGAAACAGTAGGAAAGTAGGATTATGGAGAGTTATTGTTGTCCGTAATGTTCCTTACACTGATGCAAGACGCAATGGAAAGATTCCAAAGCTTCTATTGCACAGGCTTTTCCCCAATGTCAGATATTCTATATGGATTGATGGGAAGCTTCAGCTTGTTGTGGATCCTTATCAGGTCCTTGAGAGGTTCTTGTGGCGTCAGAATGCAAATTTTGCAATCTCAAAGCACTATAGACGGTATGATGTGTTTGAAGAGGCTGAAGCTAACAGAGCTGCTGGGAAGTACGATAATTCCTCCATTAATGAGCAGGTTGACTTCTATATCAAGGAGGGTTTACAACCTTATTCTGAGGCTAAGCTTCCAATAACGAGTGATGTTCCTGAAGGTTGTGTGATCATAAAGGAACACATTCCCATTACAAATTTATTCACCTGTAATTGGTTCAATGAAGTTGATCGTTTTACCTCCAGGGATCAGTTAAGCTTTTCCACAGTGAGGGATAAAATCATGGGAAAAGTTGATTGGAGCATTAATATGTTTTTGGATTGTGAAAGGCGCAATTTTGTAATTCAGGCATACCACAGAGAGTTGCTGGAGCAGATGCCTCCTCCCCGTCCTCCGAGGGTTGTGATTCGTCGTACACCAGCTTTGCCTCATGTTATCATATCTGTAAAGACTCCACCTGGGAAGAAGCGTGGACGAGGGGATAAGAAGCGCCATCGGAAGGTTGTTGCTGTTAGTAGAGACAACATGCAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

588

Amino Acids

66.64

Weight (kDa)

9.45

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 216 - 529 2.6e-147 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 1303
AccI GTMKAC 1 cut(s) 22
AccII CGCG 1 cut(s) 112
AclWI GGATC 4 cut(s) 49, 1163, 1176, 1485
AcsI RAATTY 6 cut(s) 475, 505, 701, 991, 1213, 1426
AcuI CTGAAG 5 cut(s) 606, 996, 1139, 1284, 1407
AcyI GRCGYC 1 cut(s) 1201
AfaI GTAC 4 cut(s) 28, 636, 1289, 1645
AfiI CCNNNNNNNGG 6 cut(s) 193, 487, 536, 1443, 1500, 1729
AflIII ACRYGT 1 cut(s) 954
AgsI TTSAA 3 cut(s) 253, 1256, 1450
AjnI CCWGG 4 cut(s) 796, 1002, 1472, 1687
AjuI GAANNNNNNNTTGG 4 cut(s) 604, 636, 1510, 1542
Alw26I GTCTC 4 cut(s) 718, 842, 965, 1743
AlwI GGATC 4 cut(s) 49, 1163, 1176, 1485
AoxI GGCC 2 cut(s) 184, 489
ApeKI GCWGC 2 cut(s) 907, 1277
ApoI RAATTY 6 cut(s) 475, 505, 701, 991, 1213, 1426
AseI ATTAAT 2 cut(s) 1305, 1536
Asp700I GAANNNNTTC 4 cut(s) 118, 156, 736, 1413
AspLEI GCGC 3 cut(s) 112, 1563, 1722
AspS9I GGNCC 5 cut(s) 184, 490, 554, 766, 1180
AsuHPI GGTGA 4 cut(s) 328, 621, 812, 1426
AvaII GGWCC 3 cut(s) 554, 766, 1180
BamHI GGATCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 919, 1264
BccI CCATC 4 cut(s) 447, 501, 1139, 1731
BciT130I CCWGG 4 cut(s) 798, 1004, 1474, 1689
BciVI GTATCC 1 cut(s) 250
BclI TGATCA 1 cut(s) 1398
BcoDI GTCTC 4 cut(s) 718, 842, 965, 1743
BfaI CTAG 2 cut(s) 603, 675
BfmI CTRYAG 5 cut(s) 53, 86, 172, 597, 1234
BfoI RGCGCY 1 cut(s) 1723
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 1 cut(s) 250
BisI GCNGC 2 cut(s) 908, 1278
BlpI GCTNAGC 1 cut(s) 1359
BlsI GCNGC 2 cut(s) 909, 1279
Bme1390I CCNGG 4 cut(s) 798, 1004, 1474, 1689
Bme18I GGWCC 3 cut(s) 554, 766, 1180
BmgT120I GGNCC 5 cut(s) 184, 490, 554, 766, 1180
BmiI GGNNCC 1 cut(s) 1170
BmrFI CCNGG 4 cut(s) 798, 1004, 1474, 1689
BmsI GCATC 4 cut(s) 397, 881, 1057, 1596
BpmI CTGGAG 3 cut(s) 27, 1456, 1619
Bpu1102I GCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 1205
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 666, 1173
BsaHI GRCGYC 1 cut(s) 1201
BsaJI CCNNGG 5 cut(s) 123, 1003, 1473, 1625, 1688
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 6 cut(s) 82, 85, 112, 115, 418, 448
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 487, 536, 1443, 1500, 1729
Bse1I ACTGG 4 cut(s) 10, 569, 805, 996
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 2 cut(s) 666, 1173
BseBI CCWGG 4 cut(s) 798, 1004, 1474, 1689
BseDI CCNNGG 5 cut(s) 123, 1003, 1473, 1625, 1688
BseGI GGATG 1 cut(s) 851
BseJI GATNNNNATC 2 cut(s) 666, 1173
BseLI CCNNNNNNNGG 6 cut(s) 193, 487, 536, 1443, 1500, 1729
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 3 cut(s) 441, 713, 1344
BseNI ACTGG 4 cut(s) 10, 569, 805, 996
BseRI GAGGAG 8 cut(s) 90, 93, 420, 423, 510, 513, 516, 1602
BseXI GCAGC 2 cut(s) 919, 1264
BseYI CCCAGC 1 cut(s) 1280
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 186, 491
BsiSI CCGG 1 cut(s) 30
BslI CCNNNNNNNGG 6 cut(s) 193, 487, 536, 1443, 1500, 1729
BsmAI GTCTC 4 cut(s) 718, 842, 965, 1743
BsmI GAATGC 1 cut(s) 1213
BsnI GGCC 2 cut(s) 186, 491
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
Bsp1720I GCTNAGC 1 cut(s) 1359
BspANI GGCC 2 cut(s) 186, 491
BspCNI CTCAG 3 cut(s) 442, 714, 1345
BspFNI CGCG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 1170
BspMAI CTGCAG 1 cut(s) 90
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 4 cut(s) 49, 1163, 1176, 1485
BsrDI GCAATG 1 cut(s) 1084
BsrI ACTGG 4 cut(s) 10, 569, 805, 996
BssECI CCNNGG 5 cut(s) 123, 1003, 1473, 1625, 1688
BssMI GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
BssNI GRCGYC 1 cut(s) 1201
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 4 cut(s) 798, 1004, 1474, 1689
Bst4CI ACNGT 5 cut(s) 766, 773, 1013, 1242, 1498
Bst6I CTCTTC 2 cut(s) 963, 1251
BstACI GRCGYC 1 cut(s) 1201
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 4 cut(s) 450, 722, 1353, 1359
BstEII GGTNACC 1 cut(s) 800
BstF5I GGATG 1 cut(s) 851
BstFNI CGCG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 1723
BstHHI GCGC 3 cut(s) 112, 1563, 1722
BstKTI GATC 5 cut(s) 44, 1171, 1401, 1462, 1480
BstMAI GTCTC 4 cut(s) 718, 842, 965, 1743
BstMBI GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
BstMWI GCNNNNNNNGC 4 cut(s) 416, 1102, 1217, 1274
BstNI CCWGG 4 cut(s) 798, 1004, 1474, 1689
BstNSI RCATGY 2 cut(s) 958, 1759
BstPI GGTNACC 1 cut(s) 800
BstSCI CCNGG 4 cut(s) 796, 1002, 1472, 1687
BstSFI CTRYAG 5 cut(s) 53, 86, 172, 597, 1234
BstSNI TACGTA 1 cut(s) 26
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 919, 1264
BstX2I RGATCY 1 cut(s) 1168
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 1 cut(s) 1168
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 2 cut(s) 186, 491
BtsCI GGATG 1 cut(s) 851
BtsIMutI CAGTG 4 cut(s) 769, 1003, 1062, 1503
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 1 cut(s) 594
CfoI GCGC 3 cut(s) 112, 1563, 1722
Cfr13I GGNCC 5 cut(s) 184, 490, 554, 766, 1180
CseI GACGC 2 cut(s) 1083, 1190
CsiI ACCWGGT 1 cut(s) 796
Csp6I GTAC 4 cut(s) 27, 635, 1288, 1644
CviQI GTAC 4 cut(s) 27, 635, 1288, 1644
DdeI CTNAG 4 cut(s) 450, 722, 1353, 1359
DpnI GATC 5 cut(s) 43, 1170, 1400, 1461, 1479
DpnII GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 963, 1251
EarI CTCTTC 2 cut(s) 963, 1251
Eco105I TACGTA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 421
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 3 cut(s) 554, 766, 1180
Eco57I CTGAAG 5 cut(s) 606, 996, 1139, 1284, 1407
Eco91I GGTNACC 1 cut(s) 800
EcoO109I RGGNCCY 1 cut(s) 1180
EcoO65I GGTNACC 1 cut(s) 800
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 4 cut(s) 796, 1002, 1472, 1687
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 123
FalI AAGNNNNNCTT 4 cut(s) 604, 636, 819, 851
FauNDI CATATG 2 cut(s) 652, 742
FbaI TGATCA 1 cut(s) 1398
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 908, 1278
FokI GGATG 1 cut(s) 838
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 908, 1278
FspBI CTAG 2 cut(s) 603, 675
GlaI GCGC 3 cut(s) 111, 1562, 1721
GluI GCNGC 2 cut(s) 908, 1278
GsaI CCCAGC 1 cut(s) 1284
GsuI CTGGAG 3 cut(s) 27, 1456, 1619
HaeII RGCGCY 1 cut(s) 1723
HaeIII GGCC 2 cut(s) 186, 491
HapII CCGG 1 cut(s) 30
HgaI GACGC 2 cut(s) 1083, 1190
HhaI GCGC 3 cut(s) 112, 1563, 1722
Hin1I GRCGYC 1 cut(s) 1201
Hin6I GCGC 3 cut(s) 110, 1561, 1720
HinP1I GCGC 3 cut(s) 110, 1561, 1720
HincII GTYRAC 1 cut(s) 1318
HindII GTYRAC 1 cut(s) 1318
HindIII AAGCTT 5 cut(s) 978, 1094, 1151, 1361, 1486
HinfI GANTC 7 cut(s) 452, 626, 737, 755, 1087, 1636, 1681
HpaII CCGG 1 cut(s) 30
HphI GGTGA 4 cut(s) 328, 621, 812, 1426
Hpy166II GTNNAC 6 cut(s) 23, 769, 1318, 1340, 1646, 1703
Hpy188I TCNGA 8 cut(s) 625, 700, 723, 1127, 1206, 1354, 1626, 1728
Hpy188III TCNNGA 2 cut(s) 810, 1385
Hpy8I GTNNAC 6 cut(s) 23, 769, 1318, 1340, 1646, 1703
Hpy99I CGWCG 1 cut(s) 1644
HpyAV CCTTC 6 cut(s) 129, 142, 259, 540, 1382, 1723
HpyCH4III ACNGT 5 cut(s) 766, 773, 1013, 1242, 1498
HpyCH4IV ACGT 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 1102, 1217, 1274
HpyF3I CTNAG 4 cut(s) 450, 722, 1353, 1359
HpySE526I ACGT 1 cut(s) 25
Hsp92I GRCGYC 1 cut(s) 1201
HspAI GCGC 3 cut(s) 110, 1561, 1720
Ksp22I TGATCA 1 cut(s) 1398
Kzo9I GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
LmnI GCTCC 3 cut(s) 589, 1530, 1600
Lsp1109I GCAGC 2 cut(s) 919, 1264
LweI GCATC 4 cut(s) 397, 881, 1057, 1596
MabI ACCWGGT 1 cut(s) 796
MaeI CTAG 2 cut(s) 603, 675
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 800
MalI GATC 5 cut(s) 43, 1170, 1400, 1461, 1479
MboI GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
MboII GAAGA 8 cut(s) 365, 434, 609, 612, 980, 1000, 1268, 1705
MflI RGATCY 1 cut(s) 1168
MhlI GDGCHC 1 cut(s) 421
MlyI GAGTC 2 cut(s) 461, 1675
MmeI TCCRAC 2 cut(s) 740, 825
MroXI GAANNNNTTC 4 cut(s) 118, 156, 736, 1413
MseI TTAA 4 cut(s) 369, 1305, 1484, 1536
MslI CAYNNNNRTG 2 cut(s) 498, 1538
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 4 cut(s) 798, 1004, 1474, 1689
Mva1269I GAATGC 1 cut(s) 1213
MvaI CCWGG 4 cut(s) 798, 1004, 1474, 1689
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 4 cut(s) 416, 1102, 1217, 1274
NdeI CATATG 2 cut(s) 652, 742
NdeII GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
NlaIV GGNNCC 1 cut(s) 1170
NmuCI GTSAC 1 cut(s) 800
NspI RCATGY 2 cut(s) 958, 1759
PciI ACATGT 1 cut(s) 954
PctI GAATGC 1 cut(s) 1213
PdmI GAANNNNTTC 4 cut(s) 118, 156, 736, 1413
PfeI GAWTC 5 cut(s) 626, 737, 755, 1087, 1636
PkrI GCNGC 2 cut(s) 909, 1279
PleI GAGTC 2 cut(s) 460, 1675
PpsI GAGTC 2 cut(s) 460, 1675
Ppu21I YACGTR 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 1180
PscI ACATGT 1 cut(s) 954
PshBI ATTAAT 2 cut(s) 1305, 1536
Psp5II RGGWCCY 1 cut(s) 1180
Psp6I CCWGG 4 cut(s) 796, 1002, 1472, 1687
PspEI GGTNACC 1 cut(s) 800
PspFI CCCAGC 1 cut(s) 1280
PspGI CCWGG 4 cut(s) 796, 1002, 1472, 1687
PspN4I GGNNCC 1 cut(s) 1170
PspPI GGNCC 5 cut(s) 184, 490, 554, 766, 1180
PspPPI RGGWCCY 1 cut(s) 1180
PsrI GAACNNNNNNTAC 2 cut(s) 598, 630
PstI CTGCAG 1 cut(s) 90
PsuI RGATCY 1 cut(s) 1168
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 4 cut(s) 28, 636, 1289, 1645
RsaNI GTAC 4 cut(s) 27, 635, 1288, 1644
RseI CAYNNNNRTG 2 cut(s) 498, 1538
SaqAI TTAA 4 cut(s) 369, 1305, 1484, 1536
SatI GCNGC 2 cut(s) 908, 1278
Sau3AI GATC 5 cut(s) 41, 1168, 1398, 1459, 1477
Sau96I GGNCC 5 cut(s) 184, 490, 554, 766, 1180
SchI GAGTC 2 cut(s) 461, 1675
ScrFI CCNGG 4 cut(s) 798, 1004, 1474, 1689
SduI GDGCHC 1 cut(s) 421
SexAI ACCWGGT 1 cut(s) 796
SfaNI GCATC 4 cut(s) 397, 881, 1057, 1596
SfcI CTRYAG 5 cut(s) 53, 86, 172, 597, 1234
SinI GGWCC 3 cut(s) 554, 766, 1180
SmiMI CAYNNNNRTG 2 cut(s) 498, 1538
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
SnaBI TACGTA 1 cut(s) 26
SspMI CTAG 2 cut(s) 603, 675
StyD4I CCNGG 4 cut(s) 796, 1002, 1472, 1687
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 5 cut(s) 766, 773, 1013, 1242, 1498
TaiI ACGT 1 cut(s) 28
TaqI TCGA 3 cut(s) 292, 541, 932
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 5 cut(s) 626, 737, 755, 1087, 1636
Tru1I TTAA 4 cut(s) 369, 1305, 1484, 1536
Tru9I TTAA 4 cut(s) 369, 1305, 1484, 1536
TscAI CASTG 4 cut(s) 776, 1003, 1069, 1503
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 2 cut(s) 907, 1277
Tsp45I GTSAC 1 cut(s) 800
TspGWI ACGGA 2 cut(s) 322, 1037
TspRI CASTG 4 cut(s) 776, 1003, 1069, 1503
VpaK11BI GGWCC 3 cut(s) 554, 766, 1180
VspI ATTAAT 2 cut(s) 1305, 1536
XapI RAATTY 6 cut(s) 475, 505, 701, 991, 1213, 1426
XceI RCATGY 2 cut(s) 958, 1759
XmiI GTMKAC 1 cut(s) 22
XmnI GAANNNNTTC 4 cut(s) 118, 156, 736, 1413
XspI CTAG 2 cut(s) 603, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.