Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004914

Physical Location & Seq

Genomic Coordinates Reverse (-)

19883935 .. 19888413

Gene Structure 4479 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G53310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2904 bp

ATGGCGAATCGGAAGTTAGAGAAGATGGCATCGATTGATGTTCATCTTCGTCAACTGGTTCCTGGCAAAGTTAGTGAAGACGACAAGCTTGTTGAGTATGATGCTTTGCTTCTAGATCGGTTTCTCGATATCCTCCAGGATTTGCACGGTGAAGATCTCCGTGAAACTGTTCAAGAGCTTTATGAGCATTCTGCAGAATACGAAGGGAAGCATGAACCTAAGAAGCTAGAGGAGCTAGGGAGTGTGCTAACGAGTTTAGATCCAGGAGATTCCATTGTTATCGCTAAAGCTTTCTCTCATATGCTTAACTTAGCCAATTTGGCTGAGGAAGTGCAGATTGCTTATCGCCGTAGGATCAAGAAGCTGAAGAAAGGTGATTTTGTTGATGAGAGCTCTGCTACTACTGAATCTGATCTTGAAGAAACTTTCAAGAAGCTTGTTGGAGATCTGAACAAGTCTCCTGAAGAGATCTTTGATGCTCTCAAGAATCAGACTGTGGATTTGGTTTTGACTGCTCATCCTACTCAGTCTGTGAGAAGATCATTGCTTCAGAAACATGGGAGGATAAGAGACTGTCTGGCTCAACTATATGCTAAGGATATTACTCCTGATGACAAGCAAGAGCTCGATGAGGCTCTTCAGAGAGAGATTCAAGCTGCATTCCGAACAGATGAAATCAAAAGAACACCACCTACTCCTCAAGATGAGATGAGAGCGGGAATGAGTTATTTCCATGAAACTATCTGGAAAGGTGTTCCTAAGTTTCTGCGCCGTGTTGACACGGCTTTGAAAAACATAGGGATCGAAGAACGTGTTCCATATAATGCTCCATTGATTCAGTTCTCTTCTTGGATGGGTGGTGATCGTGACGGTAACCCAAGGGTTACACCTGAAGTCACCAGAGATGTTTGCTTGTTAGCTAGAATGATGGCTGCTACTATGTACTTTAACCAAATCGAAGATCTTATGTTTGAGATGTCTATGTGGCGTTGCAATGACGAGCTGCGTGCGCGAGCTGATGAAGTTCATGCAAATTCGAGGAAAGATGCTGCAAAACATTACATAGAATTCTGGAAGTCAATTCCTACAACTGAGCCATACCGTGTGATTCTTGGCGATGTAAGGGACAAGCTTTATCACACACGTGAACGCGCTCATCAACTGCTCAGCAATGGACACTCTGATGTCCCTGTAGAGGCTACTTTCATTAACTTGGAACAGTTCTTGGAACCTCTTGAGCTCTGTTACCGATCTCTGTGTTCATGTGGTGATCGTCCAATAGCAGATGGAAGCCTTCTTGATTTCTTGAGGCAAGTCTCAACCTTTGGGCTCTCTCTTGTGAGACTTGACATAAGGCAAGAATCTGACCGCCACACTGATGTATTGGATGCTATCACCACGCATTTAGATATCGGATCCTACAGAGAGTGGTCTGAAGAACGCCGCCAAGAATGGCTTTTATCTGAGCTAAGTGGCAAACGTCCGCTTTTCGGTTCTGATCTTCCTAAAACCGAAGAAATAGCTGATGTTCTGGACACGTTTCATGTCATAGCCGAGCTACCAGCAGATAGCTTTGGTGCTTACATTATCTCTATGGCAACTGCACCTTCTGATGTATTAGCTGTTGAGCTTTTACAGCGTGAATGCCGAGTGAAACAGCCTTTGAGAGTTGTTCCGCTCTTTGAGAAGCTAGCAGATCTGGAAGCAGCTCCTGCTGCAGTTGCTAGGCTCTTTTCTGTTGATTGGTACAAAAACCGAATTAACGGTAAGCAAGAGGTTATGATTGGTTATTCGGATTCAGGAAAAGATGCTGGACGGTTATCTGCTGCTTGGCAGTTATACAAAGCTCAAGAAGAGCTTGTGAAGGTTGCTAAAGAGTACGGTGTGAAGCTAACAATGTTTCACGGTCGTGGTGGCACGGTCGGAAGAGGAGGTGGACCAACCCATCTTGCTATATTGTCTCAGCCTCCGGATACTATTAACGGTTCCCTCCGTGTCACAGTTCAAGGTGAAGTCATCGAGCAATCGTTTGGTGAAGAGCACTTATGCTTTAGAACACTTCAGCGTTTCACAGCTGCTACACTCGAGCACGGTATGCGTCCTCCAATTTCGCCTAAACCAGAATGGCGCGCTTTGCTGGATGAAATGGCGGTTGTTGCAACCGAGGAGTATCGCTCAGTTGTGTTCCAAGAACCTCGGTTTGTCGAGTACTTCCGCCTCGCTACACCGGAACTGGAGTATGGACGTATGAATATCGGAAGCAGACCTTCGAAGCGTAAACCAAGCGGTGGCATTGAATCTCTCCGTGCAATTCCATGGATCTTCGCTTGGACTCAAACAAGATTCCATCTTCCTGTATGGCTTGGATTCGGATCAGCAATTAGACATGTGATCGAAAAAGACGTCAGGAACCTCCATATGCTCCAAGATATGTACCAACACTGGCCTTTCTTTAGAGTCACCATTGATCTAATCGAAATGGTGTTCGCTAAAGGAGATCCTGGTATTGCTGCTTTGTACGATAAGCTTCTTGTTTCAGAGGAACTCTGGCCTTTTGGTGAGAAACTCAGAGCTAACTTCGAAGAAACCAAGAAACTCATCCTCCAGACCGCTGGACACAAAGATCTTCTTGAAGGTGATCCTTACTTGAAACAGAGACTGAGACTTCGTGATTCTTACATTACAACTCTCAATGTCTGTCAAGCTTACACATTGAAGAGAATCCGTGATCCGAGTTACCATGTGACTCTGCGACCACACATTTCTAAGGAGATAGCGGAATCGAGCAAACCAGCAAAAGAACTCATCGAGCTTAACCCGACTAGCGAATACGCGCCAGGACTTGAAGATACACTCATCTTGACGATGAAGGGTATTGCTGCTGGTCTACAAAACACCGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008964 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0022607 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0051716 GO:0055044 GO:0065003 GO:0071496 GO:0071840 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

967

Amino Acids

110.28

Weight (kDa)

5.72

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 967	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53310 AT1G53310 AT1G53310 AT3G14940 AT3G14940
fragaria_vesca	FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430
malus_domestica	MD03G1242000.v1.1 MD11G1261900.v1.1
prunus_persica	Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1
pyrus_communis	pycom03g18910 pycom11g23090
rosa_chinensis	RchiOBHm_Chr5g0031161
rosa_laevigata	RLG00000033287
rosa_multiflora	Rmu_sc0011257.1_g000003
rosa_roxburghii	Rroxscaffold_1G00049100
rosa_rugosa	Rorug05G0122600 Rorug05G0122700
rosa_samantha	Rh5AG216300 Rh5BG215600 Rh5CG239100 Rh5DG220500
rosa_wichuraiana	Rw5G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2406
AccB7I	CCANNNNNTGG	1 cut(s)	2288
AccBSI	CCGCTC	2 cut(s)	716, 1678
AccI	GTMKAC	1 cut(s)	2887
AccII	CGCG	4 cut(s)	1012, 1152, 2130, 2834
AccIII	TCCGGA	1 cut(s)	1969
AcsI	RAATTY	2 cut(s)	1033, 1067
AcuI	CTGAAG	7 cut(s)	386, 483, 533, 623, 912, 1455, 2045
AcvI	CACGTG	1 cut(s)	1145
AcyI	GRCGYC	1 cut(s)	2403
AfaI	GTAC	6 cut(s)	944, 1748, 1880, 2210, 2435, 2519
AfiI	CCNNNNNNNGG	3 cut(s)	1195, 1490, 2288
AflIII	ACRYGT	4 cut(s)	811, 1142, 1536, 2386
AgeI	ACCGGT	1 cut(s)	2897
AjnI	CCWGG	5 cut(s)	61, 135, 262, 2500, 2836
AleI	CACNNNNGTG	2 cut(s)	1143, 1908
Alw21I	GWGCWC	5 cut(s)	395, 627, 1242, 2043, 2091
Alw26I	GTCTC	7 cut(s)	462, 564, 1321, 1336, 1965, 2650, 2656
AlwNI	CAGNNNCTG	2 cut(s)	1712, 2659
Ama87I	CYCGRG	1 cut(s)	2084
Aor13HI	TCCGGA	1 cut(s)	1969
AoxI	GGCC	2 cut(s)	2444, 2549
ApoI	RAATTY	2 cut(s)	1033, 1067
ArsI	GACNNNNNNTTYG	6 cut(s)	484, 516, 2388, 2420, 2649, 2681
AsiGI	ACCGGT	1 cut(s)	2897
Asp700I	GAANNNNTTC	2 cut(s)	168, 813
AspLEI	GCGC	6 cut(s)	771, 1012, 1154, 2130, 2132, 2836
AspS9I	GGNCC	1 cut(s)	1937
AsuII	TTCGAA	2 cut(s)	2270, 2580
AsuNHI	GCTAGC	1 cut(s)	1690
AvaI	CYCGRG	1 cut(s)	2084
AvaII	GGWCC	1 cut(s)	1937
BamHI	GGATCC	1 cut(s)	1415
BanII	GRGCYC	4 cut(s)	395, 627, 1242, 1332
BbrPI	CACGTG	1 cut(s)	1145
BbsI	GAAGAC	1 cut(s)	84
Bbv12I	GWGCWC	5 cut(s)	395, 627, 1242, 2043, 2091
BbvCI	CCTCAGC	1 cut(s)	324
BccI	CCATC	6 cut(s)	19, 847, 922, 1280, 1953, 2355
BceAI	ACGGC	3 cut(s)	333, 756, 798
BcgI	CGANNNNNNTGC	2 cut(s)	989, 1023
BciT130I	CCWGG	5 cut(s)	63, 137, 264, 2502, 2838
BciVI	GTATCC	1 cut(s)	1966
BcoDI	GTCTC	7 cut(s)	462, 564, 1321, 1336, 1965, 2650, 2656
BfaI	CTAG	7 cut(s)	113, 227, 236, 921, 1691, 1725, 2823
BfmI	CTRYAG	4 cut(s)	192, 1191, 1420, 1716
BfuI	GTATCC	1 cut(s)	1966
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	6 cut(s)	154, 445, 468, 961, 1696, 2623
BlpI	GCTNAGC	1 cut(s)	1166
BmcAI	AGTACT	1 cut(s)	2210
Bme1390I	CCNGG	5 cut(s)	63, 137, 264, 2502, 2838
Bme18I	GGWCC	1 cut(s)	1937
BmeT110I	CYCGRG	1 cut(s)	2084
BmgT120I	GGNCC	1 cut(s)	1937
BmiI	GGNNCC	5 cut(s)	60, 1230, 1417, 1987, 2411
BmrFI	CCNGG	5 cut(s)	63, 137, 264, 2502, 2838
BmsI	GCATC	6 cut(s)	38, 91, 466, 1036, 1378, 1798
BmtI	GCTAGC	1 cut(s)	1694
BpiI	GAAGAC	1 cut(s)	84
BplI	GAGNNNNNCTC	2 cut(s)	2159, 2191
BpmI	CTGGAG	3 cut(s)	119, 2255, 2588
Bpu10I	CCTNAGC	2 cut(s)	324, 594
Bpu1102I	GCTNAGC	1 cut(s)	1166
Bpu14I	TTCGAA	2 cut(s)	2270, 2580
BpuEI	CTTGAG	5 cut(s)	467, 684, 1256, 1327, 1833
Bsa29I	ATCGAT	1 cut(s)	32
BsaAI	YACGTR	1 cut(s)	1145
BsaBI	GATNNNNATC	2 cut(s)	42, 2371
BsaHI	GRCGYC	1 cut(s)	2403
BsaJI	CCNNGG	4 cut(s)	878, 2163, 2195, 2315
BsaWI	WCCGGW	3 cut(s)	1969, 2227, 2897
BsaXI	ACNNNNNCTCC	2 cut(s)	2586, 2616
Bsc4I	CCNNNNNNNGG	3 cut(s)	1195, 1490, 2288
Bse118I	RCCGGY	1 cut(s)	2897
Bse1I	ACTGG	3 cut(s)	60, 2238, 2447
Bse3DI	GCAATG	3 cut(s)	542, 1000, 1177
Bse8I	GATNNNNATC	2 cut(s)	42, 2371
BseAI	TCCGGA	1 cut(s)	1969
BseBI	CCWGG	5 cut(s)	63, 137, 264, 2502, 2838
BseCI	ATCGAT	1 cut(s)	32
BseDI	CCNNGG	4 cut(s)	878, 2163, 2195, 2315
BseGI	GGATG	5 cut(s)	517, 858, 1393, 2146, 2598
BseJI	GATNNNNATC	2 cut(s)	42, 2371
BseLI	CCNNNNNNNGG	3 cut(s)	1195, 1490, 2288
BseMI	GCAATG	3 cut(s)	542, 1000, 1177
BseMII	CTCAG	9 cut(s)	315, 539, 1083, 1180, 1455, 1976, 2190, 2581, 2651
BseNI	ACTGG	3 cut(s)	60, 2238, 2447
BsePI	GCGCGC	1 cut(s)	2128
BseRI	GAGGAG	4 cut(s)	245, 687, 1944, 2180
BsgI	GTGCAG	2 cut(s)	353, 1587
Bsh1236I	CGCG	4 cut(s)	1012, 1152, 2130, 2834
Bsh1285I	CGRYCG	2 cut(s)	1909, 1923
BshFI	GGCC	2 cut(s)	2446, 2551
BshTI	ACCGGT	1 cut(s)	2897
BshVI	ATCGAT	1 cut(s)	32
BsiEI	CGRYCG	2 cut(s)	1909, 1923
BsiHKAI	GWGCWC	5 cut(s)	395, 627, 1242, 2043, 2091
BsiHKCI	CYCGRG	1 cut(s)	2084
BsiSI	CCGG	3 cut(s)	1970, 2228, 2898
BslFI	GGGAC	2 cut(s)	1139, 1172
BslI	CCNNNNNNNGG	3 cut(s)	1195, 1490, 2288
BsmAI	GTCTC	7 cut(s)	462, 564, 1321, 1336, 1965, 2650, 2656
BsmFI	GGGAC	2 cut(s)	1139, 1172
BsmI	GAATGC	3 cut(s)	187, 659, 1649
BsnI	GGCC	2 cut(s)	2446, 2551
BsoBI	CYCGRG	1 cut(s)	2084
Bsp119I	TTCGAA	2 cut(s)	2270, 2580
Bsp1286I	GDGCHC	6 cut(s)	395, 627, 1242, 1332, 2043, 2091
Bsp13I	TCCGGA	1 cut(s)	1969
Bsp1720I	GCTNAGC	1 cut(s)	1166
Bsp19I	CCATGG	1 cut(s)	2315
BspANI	GGCC	2 cut(s)	2446, 2551
BspCNI	CTCAG	9 cut(s)	316, 538, 1084, 1179, 1456, 1975, 2189, 2580, 2652
BspDI	ATCGAT	1 cut(s)	32
BspEI	TCCGGA	1 cut(s)	1969
BspFNI	CGCG	4 cut(s)	1012, 1152, 2130, 2834
BspLI	GGNNCC	5 cut(s)	60, 1230, 1417, 1987, 2411
BspMAI	CTGCAG	2 cut(s)	196, 1720
BspOI	GCTAGC	1 cut(s)	1694
BspQI	GCTCTTC	3 cut(s)	642, 1848, 2031
BspT104I	TTCGAA	2 cut(s)	2270, 2580
BsrBI	CCGCTC	2 cut(s)	716, 1678
BsrDI	GCAATG	3 cut(s)	542, 1000, 1177
BsrFI	RCCGGY	1 cut(s)	2897
BsrI	ACTGG	3 cut(s)	60, 2238, 2447
BssAI	RCCGGY	1 cut(s)	2897
BssECI	CCNNGG	4 cut(s)	878, 2163, 2195, 2315
BssHII	GCGCGC	1 cut(s)	2128
BssNI	GRCGYC	1 cut(s)	2403
BssT1I	CCWWGG	2 cut(s)	878, 2315
Bst2UI	CCWGG	5 cut(s)	63, 137, 264, 2502, 2838
Bst6I	CTCTTC	7 cut(s)	459, 642, 850, 1848, 1921, 2031, 2711
BstACI	GRCGYC	1 cut(s)	2403
BstAPI	GCANNNNNTGC	2 cut(s)	1712, 2095
BstBAI	YACGTR	1 cut(s)	1145
BstBI	TTCGAA	2 cut(s)	2270, 2580
BstC8I	GCNNGC	4 cut(s)	1008, 1014, 1692, 2130
BstDSI	CCRYGG	1 cut(s)	2315
BstEII	GGTNACC	1 cut(s)	872
BstF5I	GGATG	5 cut(s)	517, 858, 1393, 2146, 2598
BstFNI	CGCG	4 cut(s)	1012, 1152, 2130, 2834
BstHHI	GCGC	6 cut(s)	771, 1012, 1154, 2130, 2132, 2836
BstMAI	GTCTC	7 cut(s)	462, 564, 1321, 1336, 1965, 2650, 2656
BstMCI	CGRYCG	2 cut(s)	1909, 1923
BstNI	CCWGG	5 cut(s)	63, 137, 264, 2502, 2838
BstNSI	RCATGY	1 cut(s)	2390
BstPI	GGTNACC	1 cut(s)	872
BstSCI	CCNGG	5 cut(s)	61, 135, 262, 2500, 2836
BstSFI	CTRYAG	4 cut(s)	192, 1191, 1420, 1716
BstUI	CGCG	4 cut(s)	1012, 1152, 2130, 2834
BstV2I	GAAGAC	1 cut(s)	84
BstXI	CCANNNNNNTGG	1 cut(s)	2612
Bsu15I	ATCGAT	1 cut(s)	32
BsuI	GTATCC	1 cut(s)	1966
BsuRI	GGCC	2 cut(s)	2446, 2551
BsuTUI	ATCGAT	1 cut(s)	32
BtgI	CCRYGG	1 cut(s)	2315
BtgZI	GCGATG	1 cut(s)	1131
BtsCI	GGATG	5 cut(s)	517, 858, 1393, 2146, 2598
BtsIMutI	CAGTG	2 cut(s)	1374, 2440
Cac8I	GCNNGC	4 cut(s)	1008, 1014, 1692, 2130
CaiI	CAGNNNCTG	2 cut(s)	1712, 2659
CfoI	GCGC	6 cut(s)	771, 1012, 1154, 2130, 2132, 2836
Cfr10I	RCCGGY	1 cut(s)	2897
Cfr13I	GGNCC	1 cut(s)	1937
ClaI	ATCGAT	1 cut(s)	32
CseI	GACGC	1 cut(s)	2087
Csp6I	GTAC	6 cut(s)	943, 1747, 1879, 2209, 2434, 2518
CspAI	ACCGGT	1 cut(s)	2897
CviAII	CATG	9 cut(s)	212, 557, 734, 1028, 1263, 1544, 2316, 2387, 2741
CviQI	GTAC	6 cut(s)	943, 1747, 1879, 2209, 2434, 2518
Eam1104I	CTCTTC	7 cut(s)	459, 642, 850, 1848, 1921, 2031, 2711
EarI	CTCTTC	7 cut(s)	459, 642, 850, 1848, 1921, 2031, 2711
EciI	GGCGGA	1 cut(s)	2204
Ecl136II	GAGCTC	3 cut(s)	393, 625, 1240
Eco130I	CCWWGG	2 cut(s)	878, 2315
Eco24I	GRGCYC	4 cut(s)	395, 627, 1242, 1332
Eco32I	GATATC	2 cut(s)	130, 1411
Eco47I	GGWCC	1 cut(s)	1937
Eco53kI	GAGCTC	3 cut(s)	393, 625, 1240
Eco57I	CTGAAG	7 cut(s)	386, 483, 533, 623, 912, 1455, 2045
Eco72I	CACGTG	1 cut(s)	1145
Eco88I	CYCGRG	1 cut(s)	2084
Eco91I	GGTNACC	1 cut(s)	872
EcoICRI	GAGCTC	3 cut(s)	393, 625, 1240
EcoO65I	GGTNACC	1 cut(s)	872
EcoRI	GAATTC	1 cut(s)	1067
EcoRII	CCWGG	5 cut(s)	61, 135, 262, 2500, 2836
EcoRV	GATATC	2 cut(s)	130, 1411
EcoT14I	CCWWGG	2 cut(s)	878, 2315
EcoT38I	GRGCYC	4 cut(s)	395, 627, 1242, 1332
ErhI	CCWWGG	2 cut(s)	878, 2315
FaeI	CATG	9 cut(s)	215, 560, 737, 1031, 1266, 1547, 2319, 2390, 2744
FalI	AAGNNNNNCTT	8 cut(s)	1440, 1472, 1842, 1874, 2251, 2283, 2613, 2645
FaqI	GGGAC	2 cut(s)	1139, 1172
FatI	CATG	9 cut(s)	211, 556, 733, 1027, 1262, 1543, 2315, 2386, 2740
FauI	CCCGC	1 cut(s)	709
FauNDI	CATATG	2 cut(s)	300, 2418
FblI	GTMKAC	1 cut(s)	2887
FokI	GGATG	5 cut(s)	504, 865, 1400, 2153, 2585
FriOI	GRGCYC	4 cut(s)	395, 627, 1242, 1332
FspBI	CTAG	7 cut(s)	113, 227, 236, 921, 1691, 1725, 2823
GlaI	GCGC	6 cut(s)	770, 1011, 1153, 2129, 2131, 2835
GsuI	CTGGAG	3 cut(s)	119, 2255, 2588
HaeIII	GGCC	2 cut(s)	2446, 2551
HapII	CCGG	3 cut(s)	1970, 2228, 2898
HgaI	GACGC	1 cut(s)	2087
HhaI	GCGC	6 cut(s)	771, 1012, 1154, 2130, 2132, 2836
Hin1I	GRCGYC	1 cut(s)	2403
Hin1II	CATG	9 cut(s)	215, 560, 737, 1031, 1266, 1547, 2319, 2390, 2744
Hin6I	GCGC	6 cut(s)	769, 1010, 1152, 2128, 2130, 2834
HinP1I	GCGC	6 cut(s)	769, 1010, 1152, 2128, 2130, 2834
HincII	GTYRAC	2 cut(s)	53, 778
HindII	GTYRAC	2 cut(s)	53, 778
HindIII	AAGCTT	6 cut(s)	86, 288, 434, 1130, 2525, 2703
HpaII	CCGG	3 cut(s)	1970, 2228, 2898
Hpy166II	GTNNAC	6 cut(s)	53, 778, 1148, 1937, 2279, 2888
Hpy8I	GTNNAC	6 cut(s)	53, 778, 1148, 1937, 2279, 2888
HpyAV	CCTTC	7 cut(s)	197, 1304, 1617, 1858, 2277, 2627, 2863
HpyCH4IV	ACGT	6 cut(s)	811, 1144, 1480, 1538, 2245, 2403
HpySE526I	ACGT	6 cut(s)	811, 1144, 1480, 1538, 2245, 2403
Hsp92I	GRCGYC	1 cut(s)	2403
Hsp92II	CATG	9 cut(s)	215, 560, 737, 1031, 1266, 1547, 2319, 2390, 2744
HspAI	GCGC	6 cut(s)	769, 1010, 1152, 2128, 2130, 2834
Kpn2I	TCCGGA	1 cut(s)	1969
LguI	GCTCTTC	3 cut(s)	642, 1848, 2031
LmnI	GCTCC	4 cut(s)	232, 832, 1714, 2427
LweI	GCATC	6 cut(s)	38, 91, 466, 1036, 1378, 1798
MaeI	CTAG	7 cut(s)	113, 227, 236, 921, 1691, 1725, 2823
MaeII	ACGT	6 cut(s)	811, 1144, 1480, 1538, 2245, 2403
MaeIII	GTNAC	9 cut(s)	866, 872, 883, 895, 1244, 1996, 2458, 2735, 2743
MbiI	CCGCTC	2 cut(s)	716, 1678
MhlI	GDGCHC	6 cut(s)	395, 627, 1242, 1332, 2043, 2091
MluCI	AATT	8 cut(s)	316, 1033, 1067, 1080, 1758, 2106, 2310, 2379
MlyI	GAGTC	3 cut(s)	2326, 2466, 2740
MmeI	TCCRAC	2 cut(s)	421, 1903
MroI	TCCGGA	1 cut(s)	1969
MroXI	GAANNNNTTC	2 cut(s)	168, 813
MseI	TTAA	7 cut(s)	306, 948, 1209, 1761, 1980, 2814, 2902
MslI	CAYNNNNRTG	4 cut(s)	1143, 1182, 1377, 1908
MspA1I	CMGCKG	2 cut(s)	2075, 2612
MspI	CCGG	3 cut(s)	1970, 2228, 2898
MspR9I	CCNGG	5 cut(s)	63, 137, 264, 2502, 2838
Mva1269I	GAATGC	3 cut(s)	187, 659, 1649
MvaI	CCWGG	5 cut(s)	63, 137, 264, 2502, 2838
MvnI	CGCG	4 cut(s)	1012, 1152, 2130, 2834
NcoI	CCATGG	1 cut(s)	2315
NdeI	CATATG	2 cut(s)	300, 2418
NheI	GCTAGC	1 cut(s)	1690
NlaIII	CATG	9 cut(s)	215, 560, 737, 1031, 1266, 1547, 2319, 2390, 2744
NlaIV	GGNNCC	5 cut(s)	60, 1230, 1417, 1987, 2411
NmeAIII	GCCGAG	2 cut(s)	1579, 1673
NmuCI	GTSAC	5 cut(s)	866, 895, 1996, 2458, 2743
NspI	RCATGY	1 cut(s)	2390
NspV	TTCGAA	2 cut(s)	2270, 2580
OliI	CACNNNNGTG	2 cut(s)	1143, 1908
PaeR7I	CTCGAG	1 cut(s)	2084
PauI	GCGCGC	1 cut(s)	2128
PciI	ACATGT	1 cut(s)	2386
PciSI	GCTCTTC	3 cut(s)	642, 1848, 2031
PcsI	WCGNNNNNNNCGW	1 cut(s)	2400
PctI	GAATGC	3 cut(s)	187, 659, 1649
PdmI	GAANNNNTTC	2 cut(s)	168, 813
PflMI	CCANNNNNTGG	1 cut(s)	2288
PfoI	TCCNGGA	2 cut(s)	135, 262
PinAI	ACCGGT	1 cut(s)	2897
PleI	GAGTC	3 cut(s)	2326, 2465, 2740
PmaCI	CACGTG	1 cut(s)	1145
PmlI	CACGTG	1 cut(s)	1145
PpsI	GAGTC	3 cut(s)	2326, 2465, 2740
Ppu21I	YACGTR	1 cut(s)	1145
PscI	ACATGT	1 cut(s)	2386
Psp124BI	GAGCTC	3 cut(s)	395, 627, 1242
Psp6I	CCWGG	5 cut(s)	61, 135, 262, 2500, 2836
PspCI	CACGTG	1 cut(s)	1145
PspEI	GGTNACC	1 cut(s)	872
PspGI	CCWGG	5 cut(s)	61, 135, 262, 2500, 2836
PspN4I	GGNNCC	5 cut(s)	60, 1230, 1417, 1987, 2411
PspPI	GGNCC	1 cut(s)	1937
PspXI	VCTCGAGB	1 cut(s)	2084
PsrI	GAACNNNNNNTAC	2 cut(s)	676, 708
PstI	CTGCAG	2 cut(s)	196, 1720
PstNI	CAGNNNCTG	2 cut(s)	1712, 2659
PteI	GCGCGC	1 cut(s)	2128
PvuII	CAGCTG	1 cut(s)	2075
RsaI	GTAC	6 cut(s)	944, 1748, 1880, 2210, 2435, 2519
RsaNI	GTAC	6 cut(s)	943, 1747, 1879, 2209, 2434, 2518
RseI	CAYNNNNRTG	4 cut(s)	1143, 1182, 1377, 1908
SacI	GAGCTC	3 cut(s)	395, 627, 1242
SapI	GCTCTTC	3 cut(s)	642, 1848, 2031
SaqAI	TTAA	7 cut(s)	306, 948, 1209, 1761, 1980, 2814, 2902
Sau96I	GGNCC	1 cut(s)	1937
ScaI	AGTACT	1 cut(s)	2210
SchI	GAGTC	3 cut(s)	2326, 2466, 2740
ScrFI	CCNGG	5 cut(s)	63, 137, 264, 2502, 2838
SduI	GDGCHC	6 cut(s)	395, 627, 1242, 1332, 2043, 2091
SfaNI	GCATC	6 cut(s)	38, 91, 466, 1036, 1378, 1798
SfcI	CTRYAG	4 cut(s)	192, 1191, 1420, 1716
Sfr274I	CTCGAG	1 cut(s)	2084
SfuI	TTCGAA	2 cut(s)	2270, 2580
SinI	GGWCC	1 cut(s)	1937
SlaI	CTCGAG	1 cut(s)	2084
SmiMI	CAYNNNNRTG	4 cut(s)	1143, 1182, 1377, 1908
SmlI	CTYRAG	6 cut(s)	482, 699, 1235, 1306, 1848, 2084
SmoI	CTYRAG	6 cut(s)	482, 699, 1235, 1306, 1848, 2084
Sse9I	AATT	8 cut(s)	316, 1033, 1067, 1080, 1758, 2106, 2310, 2379
SspMI	CTAG	7 cut(s)	113, 227, 236, 921, 1691, 1725, 2823
SstI	GAGCTC	3 cut(s)	395, 627, 1242
StyD4I	CCNGG	5 cut(s)	61, 135, 262, 2500, 2836
StyI	CCWWGG	2 cut(s)	878, 2315
TaiI	ACGT	6 cut(s)	814, 1147, 1483, 1541, 2248, 2406
TasI	AATT	8 cut(s)	316, 1033, 1067, 1080, 1758, 2106, 2310, 2379
TatI	WGTACW	2 cut(s)	942, 2208
TauI	GCSGC	1 cut(s)	1446
Tru1I	TTAA	7 cut(s)	306, 948, 1209, 1761, 1980, 2814, 2902
Tru9I	TTAA	7 cut(s)	306, 948, 1209, 1761, 1980, 2814, 2902
TscAI	CASTG	2 cut(s)	1381, 2447
TseFI	GTSAC	5 cut(s)	866, 895, 1996, 2458, 2743
Tsp45I	GTSAC	5 cut(s)	866, 895, 1996, 2458, 2743
TspGWI	ACGGA	4 cut(s)	149, 1982, 2294, 2714
TspRI	CASTG	2 cut(s)	1381, 2447
Van91I	CCANNNNNTGG	1 cut(s)	2288
VpaK11BI	GGWCC	1 cut(s)	1937
XapI	RAATTY	2 cut(s)	1033, 1067
XbaI	TCTAGA	1 cut(s)	112
XceI	RCATGY	1 cut(s)	2390
XhoI	CTCGAG	1 cut(s)	2084
XmiI	GTMKAC	1 cut(s)	2887
XmnI	GAANNNNTTC	2 cut(s)	168, 813
XspI	CTAG	7 cut(s)	113, 227, 236, 921, 1691, 1725, 2823
ZraI	GACGTC	1 cut(s)	2404
ZrmI	AGTACT	1 cut(s)	2210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.