Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004914

Physical Location & Seq

Genomic Coordinates Reverse (-)

37648687 .. 37655099

Gene Structure 6413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1261900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2907 bp

ATGGCTAACAGGAATCTGGAGAAGATGGCGTCCATTGACGCGCAGCTTCGGCTTCTGGTTCCGGCCAAAGTGAGCGAGGATGACAAGCTGGTGGAATACGATGCTTTGCTTCTGGATCGGTTTCTGGATATTCTTCAAGATTTACATGGGGAGGATCTCAGGGAAACGGTTCAAGCATGTTATGAGCTTTCTGCTGAGTATGAAGGAAAGCATGACCCGAAAAAGCTGTCAGAGCTTGGAAATGTATTAACAAGTTTGGACCCAGGTGATTCTATTGTTATTGCAAAATCCTTCGCCCACATGCTTAGCTTGGCCAACTTGGCTGAGGAGGTCCAGATTGCTTACCGCAGACGACAAAAGCTCAAAAAGGGTGACTTTGCTGATGAAAATTCTGCAACGACTGAATCAGACATTGAAGAAACTCTCAAGAGGCTTGTGGGGGATCTCAAAAAGTCTCCCCAAGAGGTTTTTGATGCTCTTAAAAATCAGACTGTTGATTTGGTCCTCACTGCTCATCCTACACAATCAGTTCGTAGATCCTTACTTCAGAAGCATGGAAGGATTCGGAATTGTTTAACCCAGTTGTATGCGAAAGACATTACTCCTGATGATAAGCAGGAGATTGATGAGGCACTTCAGAGAGAGATCCAAGCTGCATTTCGTACTGATGAGATCCGAAGAACAGCTCCCACACCACAAGATGAAATGAGAGCAGGGATGAGCTATTTCCATGAAACAATTTGGAAGGGTGTCCCAAAGTTCCTACGTCGTGTTGACACAGCTTTGAAGAATATTGGGATTAACGAACGGGTTCCTCATAATGCCCCACTTATTCAGTTTTCTTCTTGGATGGGTGGTGATCGTGATGGCAATCCAAGAGTAACTCCTGAGGTAACAAGGGATGTTTGCCTACTGGCTAGAATGATGGCTTCAAACTTGTACTATTCTCAGATAGAGGATCTTATGTTTGAGTGGGATTTTTTGTCTATGTGGCGGTGCAATGATGAACTCCGTGTTCGTGCAGATGTACTTCACAGGTCCACGAGGAGAGATGCAAAGCACTACATAGAATTTTGGAAGCAAGTTCCTCCAAATGAACCCTACCGTGTGATTCTCGGTGATGTAAGGGATAAGCTTTATCATACACGTGAACGTTCTCGTCAGTTGTTAGCCAGTGGGCACTCTGACATTCCAGAGGAGGCAACACTGATTAGTGTTGAGCAGTTCTTGGAACCGCTCGAACTCTGTTACAGATCACTCTGCTCTTGTGGTGACCGGGCAATTGCTGATGGATCACTTCTTGATTTCTTAAGGCAAGTGTCTACCTTTGGACTTTCACTTGTGAAGCTCGATATTAGGCAAGAATCTGACAGGCATACTGATGTGATTGATGCCATCACCAAGCATCTGGAAATTGGTTCCTATCGTGAGTGGTCTGAAGAACGCCGTCAGGAGTGGCTTTTATCTGAACTGTGTGGCAAGCGCCCACTGTTTGGCCCTGACCTTCCAAAAACGGAAGAAATTTCTGATGTTTTGGACACATTACATGTGATATCAGAACTTCCATCTGATAACTTTGGAGCATACATCATATCAATGGCGACTGCTCCATCTGATGTGCTTGCAGTTGAGCTCCTGCAACGTGAATGCCATGTGAAGAAACCACTGAGAGTTGTTCCACTGTTTGAGAAGCTTGCAGATCTGGAGGCTGCTCCTGCTGCTTTGGCTCGGCTTTTCTCGATTGATTGGTATAGAGAGCGAATCAATGGAAAGCAAGAAGTCATGATTGGGTACTCAGATTCTGGCAAAGATGCTGGGCGTTTCTCTGCAGCATGGCAGTTATATAAGGCACAGGAGGAGCTTATAAACGTTGCTAAGAAATTTGGTGTGAAGCTAACTATGTTCCATGGTCGTGGTGGCACTGTTGGAAGGGGAGGTGGGCCCACCCATCTTGCTATATTGTCTCAACCACCAGATACGATTCATGGTTCACTCCGTGTCACTGTCCAAGGTGAAGTTATTGAGCAGTCGTTTGGAGAGGAGCTCTTGTGTTTTAGAACACTCCAGCGTTTCACCGCTGCTACATTAGAGCACGGAATGCATATGCCAGTTTCACCAAAACCGGAATGGCGTGCACTTATGGACGAAATGGCTGTTGTAGCTACAGAGGACTATCGTTCCATTGTTTTCCAAGAACCCCGATTTGTCGAGTACTTCCGTCTTGCTACACCGGAGCTGGAGTATGGTCGGATGAACATTGGCAGTCGCCCCTCAAAGCGAAAGCCTAGTGGAGGCATTGAGTCCCTTCGTGCAATCCCATGGATTTTCGCCTGGACACAAACAAGGTTCCATCTTCCAGTGTGGTTAGGCTTCGGAGCAGCCTTCAGACATGTCATTAAGAAGGACATTAAGAATCTTCACATGCTGCAAGAGATGTACAACCAATGGCCTTTCTTCAGGGTCACTATTGATTTGGTTGAAATGGTGTTTGCCAAGGGAAACCCCGGAATAGCTGCGTTATATGATAAACTCCTTGTTTCAGAAGACCTATGGTCATTCGGAGAGCTATTACGGTCCAACTATGAAGAAACCAAGAACTTTCTGTTACAGATTGCTGGACACAAGGATCTTCTCGAAGGTGACCCGCACTTGAAGCAAAGACTCCGCCTGCGTGATTCATACATCACAACCCTCAATGTTTGCCAAGCATATACACTGAAACGGATCCGTGATCCAAACTTCCACGTGACACTGCGGCCACACATCTCAAAGGAAATCAACGAAACAAACAAGCCGGCTAATGAACTTGTAAAGCTGAACCCAACAAGCGAGTACGCGCCTGGTCTGGAGGACACCCTCATCTTGACAATGAAGGGTATTGCTGCCGGCTTACAGAACACCGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008964 GO:0009267 GO:0009507 GO:0009536 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0051716 GO:0065003 GO:0071496 GO:0071840 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

969

Amino Acids

110.61

Weight (kDa)

6.0

Isoelectric Point (pI)

47.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 968	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53310 AT1G53310 AT1G53310 AT3G14940 AT3G14940
fragaria_vesca	FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430
malus_domestica	MD03G1242000.v1.1 MD11G1261900.v1.1
prunus_persica	Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1
pyrus_communis	pycom03g18910 pycom11g23090
rosa_chinensis	RchiOBHm_Chr5g0031161
rosa_laevigata	RLG00000033287
rosa_multiflora	Rmu_sc0011257.1_g000003
rosa_roxburghii	Rroxscaffold_1G00049100
rosa_rugosa	Rorug05G0122600 Rorug05G0122700
rosa_samantha	Rh5AG216300 Rh5BG215600 Rh5CG239100 Rh5DG220500
rosa_wichuraiana	Rw5G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1865
AccB7I	CCANNNNNTGG	1 cut(s)	1493
AccBSI	CCGCTC	1 cut(s)	1237
AccI	GTMKAC	1 cut(s)	1322
AccII	CGCG	2 cut(s)	41, 2837
AciI	CCGC	7 cut(s)	346, 994, 1235, 2076, 2645, 2665, 2755
AclI	AACGTT	2 cut(s)	1153, 1869
AcoI	YGGCCR	3 cut(s)	63, 312, 2756
AcsI	RAATTY	4 cut(s)	388, 1070, 1521, 1880
AcuI	CTGAAG	5 cut(s)	530, 620, 1458, 2368, 2440
AcvI	CACGTG	2 cut(s)	1148, 2746
AcyI	GRCGYC	1 cut(s)	29
AdeI	CACNNNGTG	1 cut(s)	1997
AfaI	GTAC	7 cut(s)	664, 941, 1029, 1793, 2213, 2438, 2834
AfiI	CCNNNNNNNGG	2 cut(s)	1493, 2291
AflII	CTTAAG	1 cut(s)	1309
AflIII	ACRYGT	3 cut(s)	1145, 1546, 2389
AgeI	ACCGGT	1 cut(s)	2900
AgsI	TTSAA	7 cut(s)	137, 173, 416, 787, 933, 2480, 2653
AhdI	GACNNNNNGTC	1 cut(s)	2551
AjnI	CCWGG	3 cut(s)	262, 2330, 2839
AjuI	GAANNNNNNNTTGG	4 cut(s)	642, 674, 1770, 1802
Alw21I	GWGCWC	4 cut(s)	1635, 2046, 2094, 2137
Alw26I	GTCTC	2 cut(s)	459, 1968
Alw44I	GTGCAC	1 cut(s)	2133
AlwNI	CAGNNNCTG	1 cut(s)	1802
AoxI	GGCC	6 cut(s)	63, 312, 1495, 1940, 2447, 2756
ApaI	GGGCCC	1 cut(s)	1944
ApaLI	GTGCAC	1 cut(s)	2133
ApoI	RAATTY	4 cut(s)	388, 1070, 1521, 1880
ArsI	GACNNNNNNTTYG	4 cut(s)	212, 244, 481, 513
AsiGI	ACCGGT	1 cut(s)	2900
Asp700I	GAANNNNTTC	2 cut(s)	168, 810
AspLEI	GCGC	3 cut(s)	43, 1485, 2839
AspS9I	GGNCC	8 cut(s)	259, 331, 502, 1038, 1496, 1940, 1941, 2574
AsuC2I	CCSGG	2 cut(s)	1277, 2505
AvaII	GGWCC	5 cut(s)	259, 331, 502, 1038, 2574
AxyI	CCTNAGG	1 cut(s)	888
BaeGI	GKGCMC	3 cut(s)	1182, 1944, 2137
BalI	TGGCCA	1 cut(s)	314
BamHI	GGATCC	1 cut(s)	2724
BanII	GRGCYC	3 cut(s)	1635, 1944, 2046
BauI	CACGAG	1 cut(s)	1042
BbrPI	CACGTG	2 cut(s)	1148, 2746
BbsI	GAAGAC	1 cut(s)	2550
Bbv12I	GWGCWC	4 cut(s)	1635, 2046, 2094, 2137
BbvCI	CCTCAGC	1 cut(s)	324
BceAI	ACGGC	1 cut(s)	1431
BciT130I	CCWGG	3 cut(s)	264, 2332, 2841
BcnI	CCSGG	2 cut(s)	1277, 2505
BcoDI	GTCTC	2 cut(s)	459, 1968
BfaI	CTAG	2 cut(s)	918, 2286
BfmI	CTRYAG	2 cut(s)	1827, 2163
BfoI	RGCGCY	1 cut(s)	1486
BfrI	CTTAAG	1 cut(s)	1309
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	1 cut(s)	1699
BlpI	GCTNAGC	1 cut(s)	305
BmcAI	AGTACT	1 cut(s)	2213
Bme1390I	CCNGG	5 cut(s)	264, 1277, 2332, 2505, 2841
Bme18I	GGWCC	5 cut(s)	259, 331, 502, 1038, 2574
BmeRI	GACNNNNNGTC	1 cut(s)	2551
BmgT120I	GGNCC	8 cut(s)	259, 331, 502, 1038, 1496, 1940, 1941, 2574
BmiI	GGNNCC	8 cut(s)	60, 261, 813, 1233, 1420, 1942, 2348, 2726
BmrFI	CCNGG	5 cut(s)	264, 1277, 2332, 2505, 2841
BmrI	ACTGGG	1 cut(s)	574
BmsI	GCATC	6 cut(s)	91, 463, 1042, 1381, 1414, 1801
BmuI	ACTGGG	1 cut(s)	574
BpiI	GAAGAC	1 cut(s)	2550
BplI	GAGNNNNNCTC	2 cut(s)	2028, 2060
BpmI	CTGGAG	5 cut(s)	38, 1724, 2048, 2258, 2867
Bpu10I	CCTNAGC	1 cut(s)	324
Bpu1102I	GCTNAGC	1 cut(s)	305
BpuEI	CTTGAG	1 cut(s)	410
BpuMI	CCSGG	2 cut(s)	1277, 2505
BsaAI	YACGTR	2 cut(s)	1148, 2746
BsaBI	GATNNNNATC	1 cut(s)	870
BsaHI	GRCGYC	1 cut(s)	29
BsaJI	CCNNGG	6 cut(s)	262, 1906, 2008, 2318, 2493, 2503
BsaWI	WCCGGW	3 cut(s)	2122, 2230, 2900
BsaXI	ACNNNNNCTCC	4 cut(s)	2033, 2063, 2225, 2255
Bsc4I	CCNNNNNNNGG	2 cut(s)	1493, 2291
Bse118I	RCCGGY	3 cut(s)	2794, 2885, 2900
Bse1I	ACTGG	5 cut(s)	580, 918, 1173, 2108, 2357
Bse21I	CCTNAGG	1 cut(s)	888
Bse3DI	GCAATG	1 cut(s)	1006
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	3 cut(s)	264, 2332, 2841
BseDI	CCNNGG	6 cut(s)	262, 1906, 2008, 2318, 2493, 2503
BseGI	GGATG	6 cut(s)	85, 514, 723, 855, 907, 2256
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	2 cut(s)	1493, 2291
BseMI	GCAATG	1 cut(s)	1006
BseMII	CTCAG	7 cut(s)	172, 186, 315, 879, 962, 1658, 1809
BseNI	ACTGG	5 cut(s)	580, 918, 1173, 2108, 2357
BseRI	GAGGAG	5 cut(s)	341, 1060, 1211, 1871, 2054
BseSI	GKGCMC	3 cut(s)	1182, 1944, 2137
BseYI	CCCAGC	1 cut(s)	1814
BsgI	GTGCAG	1 cut(s)	1041
Bsh1236I	CGCG	2 cut(s)	41, 2837
BshFI	GGCC	6 cut(s)	65, 314, 1497, 1942, 2449, 2758
BshTI	ACCGGT	1 cut(s)	2900
BsiHKAI	GWGCWC	4 cut(s)	1635, 2046, 2094, 2137
BsiSI	CCGG	8 cut(s)	62, 1276, 2123, 2231, 2505, 2795, 2886, 2901
BslFI	GGGAC	2 cut(s)	737, 2287
BslI	CCNNNNNNNGG	2 cut(s)	1493, 2291
BsmAI	GTCTC	2 cut(s)	459, 1968
BsmFI	GGGAC	2 cut(s)	737, 2287
BsmI	GAATGC	2 cut(s)	1652, 2103
BsnI	GGCC	6 cut(s)	65, 314, 1497, 1942, 2449, 2758
Bsp120I	GGGCCC	1 cut(s)	1940
Bsp1286I	GDGCHC	6 cut(s)	1182, 1635, 1944, 2046, 2094, 2137
Bsp1407I	TGTACA	1 cut(s)	2436
Bsp1720I	GCTNAGC	1 cut(s)	305
Bsp19I	CCATGG	2 cut(s)	1906, 2318
BspACI	CCGC	7 cut(s)	346, 994, 1235, 2076, 2645, 2665, 2755
BspANI	GGCC	6 cut(s)	65, 314, 1497, 1942, 2449, 2758
BspCNI	CTCAG	7 cut(s)	171, 187, 316, 880, 961, 1659, 1808
BspFNI	CGCG	2 cut(s)	41, 2837
BspHI	TCATGA	1 cut(s)	1782
BspLI	GGNNCC	8 cut(s)	60, 261, 813, 1233, 1420, 1942, 2348, 2726
BspMAI	CTGCAG	1 cut(s)	1831
BspTI	CTTAAG	1 cut(s)	1309
BsrBI	CCGCTC	1 cut(s)	1237
BsrDI	GCAATG	1 cut(s)	1006
BsrFI	RCCGGY	3 cut(s)	2794, 2885, 2900
BsrGI	TGTACA	1 cut(s)	2436
BsrI	ACTGG	5 cut(s)	580, 918, 1173, 2108, 2357
BssAI	RCCGGY	3 cut(s)	2794, 2885, 2900
BssECI	CCNNGG	6 cut(s)	262, 1906, 2008, 2318, 2493, 2503
BssNI	GRCGYC	1 cut(s)	29
BssSI	CACGAG	1 cut(s)	1042
BssT1I	CCWWGG	4 cut(s)	1906, 2008, 2318, 2493
Bst2BI	CACGAG	1 cut(s)	1042
Bst2UI	CCWGG	3 cut(s)	264, 2332, 2841
Bst4CI	ACNGT	9 cut(s)	169, 493, 1106, 1473, 1491, 1683, 1924, 2005, 2574
BstACI	GRCGYC	1 cut(s)	29
BstAFI	CTTAAG	1 cut(s)	1309
BstAPI	GCANNNNNTGC	1 cut(s)	2098
BstAUI	TGTACA	1 cut(s)	2436
BstBAI	YACGTR	2 cut(s)	1148, 2746
BstC8I	GCNNGC	7 cut(s)	1481, 1623, 1695, 2133, 2669, 2796, 2887
BstDEI	CTNAG	9 cut(s)	158, 195, 305, 324, 888, 948, 1667, 1795, 1875
BstDSI	CCRYGG	2 cut(s)	1906, 2318
BstEII	GGTNACC	2 cut(s)	1271, 2639
BstENI	CCTNNNNNAGG	1 cut(s)	2289
BstF5I	GGATG	6 cut(s)	85, 514, 723, 855, 907, 2256
BstFNI	CGCG	2 cut(s)	41, 2837
BstH2I	RGCGCY	1 cut(s)	1486
BstHHI	GCGC	3 cut(s)	43, 1485, 2839
BstMAI	GTCTC	2 cut(s)	459, 1968
BstMWI	GCNNNNNNNGC	9 cut(s)	49, 232, 320, 1715, 1718, 1724, 2098, 2159, 2653
BstNI	CCWGG	3 cut(s)	264, 2332, 2841
BstNSI	RCATGY	5 cut(s)	180, 304, 1550, 2393, 2425
BstPI	GGTNACC	2 cut(s)	1271, 2639
BstSCI	CCNGG	5 cut(s)	262, 1275, 2330, 2503, 2839
BstSFI	CTRYAG	2 cut(s)	1827, 2163
BstSLI	GKGCMC	3 cut(s)	1182, 1944, 2137
BstUI	CGCG	2 cut(s)	41, 2837
BstV2I	GAAGAC	1 cut(s)	2550
BstX2I	RGATCY	9 cut(s)	154, 442, 536, 645, 672, 958, 1699, 2626, 2724
BstXI	CCANNNNNNTGG	2 cut(s)	1408, 1913
BstYI	RGATCY	9 cut(s)	154, 442, 536, 645, 672, 958, 1699, 2626, 2724
Bsu36I	CCTNAGG	1 cut(s)	888
BsuRI	GGCC	6 cut(s)	65, 314, 1497, 1942, 2449, 2758
BtgI	CCRYGG	2 cut(s)	1906, 2318
BtsCI	GGATG	6 cut(s)	85, 514, 723, 855, 907, 2256
BtsI	GCAGTG	2 cut(s)	507, 2750
Cac8I	GCNNGC	7 cut(s)	1481, 1623, 1695, 2133, 2669, 2796, 2887
CaiI	CAGNNNCTG	1 cut(s)	1802
CciI	TCATGA	1 cut(s)	1782
CfoI	GCGC	3 cut(s)	43, 1485, 2839
Cfr10I	RCCGGY	3 cut(s)	2794, 2885, 2900
Cfr13I	GGNCC	8 cut(s)	259, 331, 502, 1038, 1496, 1940, 1941, 2574
CseI	GACGC	2 cut(s)	18, 47
Csp6I	GTAC	7 cut(s)	663, 940, 1028, 1792, 2212, 2437, 2833
CspAI	ACCGGT	1 cut(s)	2900
CspCI	CAANNNNNGTGG	2 cut(s)	1933, 1968
CviQI	GTAC	7 cut(s)	663, 940, 1028, 1792, 2212, 2437, 2833
DdeI	CTNAG	9 cut(s)	158, 195, 305, 324, 888, 948, 1667, 1795, 1875
DraIII	CACNNNGTG	1 cut(s)	1997
DriI	GACNNNNNGTC	1 cut(s)	2551
EaeI	YGGCCR	3 cut(s)	63, 312, 2756
Eam1105I	GACNNNNNGTC	1 cut(s)	2551
EciI	GGCGGA	1 cut(s)	2654
Ecl136II	GAGCTC	2 cut(s)	1633, 2044
Eco130I	CCWWGG	4 cut(s)	1906, 2008, 2318, 2493
Eco24I	GRGCYC	3 cut(s)	1635, 1944, 2046
Eco32I	GATATC	1 cut(s)	1554
Eco47I	GGWCC	5 cut(s)	259, 331, 502, 1038, 2574
Eco53kI	GAGCTC	2 cut(s)	1633, 2044
Eco57I	CTGAAG	5 cut(s)	530, 620, 1458, 2368, 2440
Eco72I	CACGTG	2 cut(s)	1148, 2746
Eco81I	CCTNAGG	1 cut(s)	888
Eco91I	GGTNACC	2 cut(s)	1271, 2639
EcoICRI	GAGCTC	2 cut(s)	1633, 2044
EcoNI	CCTNNNNNAGG	1 cut(s)	2289
EcoO65I	GGTNACC	2 cut(s)	1271, 2639
EcoRII	CCWGG	3 cut(s)	262, 2330, 2839
EcoRV	GATATC	1 cut(s)	1554
EcoT14I	CCWWGG	4 cut(s)	1906, 2008, 2318, 2493
EcoT22I	ATGCAT	1 cut(s)	2103
EcoT38I	GRGCYC	3 cut(s)	1635, 1944, 2046
ErhI	CCWWGG	4 cut(s)	1906, 2008, 2318, 2493
FalI	AAGNNNNNCTT	2 cut(s)	359, 391
FaqI	GGGAC	2 cut(s)	737, 2287
FauI	CCCGC	1 cut(s)	2652
FauNDI	CATATG	1 cut(s)	2103
FblI	GTMKAC	1 cut(s)	1322
FokI	GGATG	6 cut(s)	92, 501, 730, 862, 914, 2263
FriOI	GRGCYC	3 cut(s)	1635, 1944, 2046
FspBI	CTAG	2 cut(s)	918, 2286
GlaI	GCGC	3 cut(s)	42, 1484, 2838
GsaI	CCCAGC	1 cut(s)	1818
GsuI	CTGGAG	5 cut(s)	38, 1724, 2048, 2258, 2867
HaeII	RGCGCY	1 cut(s)	1486
HaeIII	GGCC	6 cut(s)	65, 314, 1497, 1942, 2449, 2758
HapII	CCGG	8 cut(s)	62, 1276, 2123, 2231, 2505, 2795, 2886, 2901
HgaI	GACGC	2 cut(s)	18, 47
HhaI	GCGC	3 cut(s)	43, 1485, 2839
Hin1I	GRCGYC	1 cut(s)	29
Hin6I	GCGC	3 cut(s)	41, 1483, 2837
HinP1I	GCGC	3 cut(s)	41, 1483, 2837
HincII	GTYRAC	1 cut(s)	775
HindII	GTYRAC	1 cut(s)	775
HindIII	AAGCTT	2 cut(s)	1133, 1691
HpaII	CCGG	8 cut(s)	62, 1276, 2123, 2231, 2505, 2795, 2886, 2901
Hpy166II	GTNNAC	6 cut(s)	775, 1041, 1151, 1323, 1991, 2135
Hpy8I	GTNNAC	6 cut(s)	775, 1041, 1151, 1323, 1991, 2135
Hpy99I	CGWCG	1 cut(s)	771
HpyCH4III	ACNGT	9 cut(s)	169, 493, 1106, 1473, 1491, 1683, 1924, 2005, 2574
HpyCH4IV	ACGT	6 cut(s)	766, 1147, 1153, 1642, 1869, 2745
HpyF10VI	GCNNNNNNNGC	9 cut(s)	49, 232, 320, 1715, 1718, 1724, 2098, 2159, 2653
HpyF3I	CTNAG	9 cut(s)	158, 195, 305, 324, 888, 948, 1667, 1795, 1875
HpySE526I	ACGT	6 cut(s)	766, 1147, 1153, 1642, 1869, 2745
Hsp92I	GRCGYC	1 cut(s)	29
HspAI	GCGC	3 cut(s)	41, 1483, 2837
KroI	GCCGGC	2 cut(s)	2794, 2885
KroNI	GCCGGC	2 cut(s)	2796, 2887
LmnI	GCTCC	9 cut(s)	691, 1580, 1612, 1638, 1717, 1858, 2041, 2233, 2375
LweI	GCATC	6 cut(s)	91, 463, 1042, 1381, 1414, 1801
MaeI	CTAG	2 cut(s)	918, 2286
MaeII	ACGT	6 cut(s)	766, 1147, 1153, 1642, 1869, 2745
MbiI	CCGCTC	1 cut(s)	1237
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	9 cut(s)	154, 442, 536, 645, 672, 958, 1699, 2626, 2724
MhlI	GDGCHC	6 cut(s)	1182, 1635, 1944, 2046, 2094, 2137
MlsI	TGGCCA	1 cut(s)	314
MluCI	AATT	8 cut(s)	388, 568, 738, 1070, 1281, 1413, 1521, 1880
MluNI	TGGCCA	1 cut(s)	314
MlyI	GAGTC	2 cut(s)	2309, 2655
MmeI	TCCRAC	3 cut(s)	1906, 2228, 2601
Mox20I	TGGCCA	1 cut(s)	314
Mph1103I	ATGCAT	1 cut(s)	2103
MroNI	GCCGGC	2 cut(s)	2794, 2885
MroXI	GAANNNNTTC	2 cut(s)	168, 810
MscI	TGGCCA	1 cut(s)	314
MseI	TTAA	8 cut(s)	248, 480, 575, 801, 1310, 2397, 2409, 2905
MslI	CAYNNNNRTG	4 cut(s)	1146, 1380, 1595, 1911
Msp20I	TGGCCA	1 cut(s)	314
MspA1I	CMGCKG	1 cut(s)	2078
MspCI	CTTAAG	1 cut(s)	1309
MspI	CCGG	8 cut(s)	62, 1276, 2123, 2231, 2505, 2795, 2886, 2901
MspR9I	CCNGG	5 cut(s)	264, 1277, 2332, 2505, 2841
MunI	CAATTG	1 cut(s)	1281
Mva1269I	GAATGC	2 cut(s)	1652, 2103
MvaI	CCWGG	3 cut(s)	264, 2332, 2841
MvnI	CGCG	2 cut(s)	41, 2837
MwoI	GCNNNNNNNGC	9 cut(s)	49, 232, 320, 1715, 1718, 1724, 2098, 2159, 2653
NaeI	GCCGGC	2 cut(s)	2796, 2887
NciI	CCSGG	2 cut(s)	1277, 2505
NcoI	CCATGG	2 cut(s)	1906, 2318
NdeI	CATATG	1 cut(s)	2103
NgoMIV	GCCGGC	2 cut(s)	2794, 2885
NlaIV	GGNNCC	8 cut(s)	60, 261, 813, 1233, 1420, 1942, 2348, 2726
NmeAIII	GCCGAG	1 cut(s)	1708
NmuCI	GTSAC	6 cut(s)	371, 1271, 1999, 2461, 2639, 2746
NsiI	ATGCAT	1 cut(s)	2103
NspI	RCATGY	5 cut(s)	180, 304, 1550, 2393, 2425
PagI	TCATGA	1 cut(s)	1782
PciI	ACATGT	2 cut(s)	1546, 2389
PctI	GAATGC	2 cut(s)	1652, 2103
PdiI	GCCGGC	2 cut(s)	2796, 2887
PdmI	GAANNNNTTC	2 cut(s)	168, 810
PflMI	CCANNNNNTGG	1 cut(s)	1493
PinAI	ACCGGT	1 cut(s)	2900
PleI	GAGTC	2 cut(s)	2308, 2655
PmaCI	CACGTG	2 cut(s)	1148, 2746
PmlI	CACGTG	2 cut(s)	1148, 2746
PpsI	GAGTC	2 cut(s)	2308, 2655
Ppu21I	YACGTR	2 cut(s)	1148, 2746
PscI	ACATGT	2 cut(s)	1546, 2389
PsiI	TTATAA	1 cut(s)	1865
Psp124BI	GAGCTC	2 cut(s)	1635, 2046
Psp1406I	AACGTT	2 cut(s)	1153, 1869
Psp6I	CCWGG	3 cut(s)	262, 2330, 2839
PspCI	CACGTG	2 cut(s)	1148, 2746
PspEI	GGTNACC	2 cut(s)	1271, 2639
PspFI	CCCAGC	1 cut(s)	1814
PspGI	CCWGG	3 cut(s)	262, 2330, 2839
PspN4I	GGNNCC	8 cut(s)	60, 261, 813, 1233, 1420, 1942, 2348, 2726
PspOMI	GGGCCC	1 cut(s)	1940
PspPI	GGNCC	8 cut(s)	259, 331, 502, 1038, 1496, 1940, 1941, 2574
PstI	CTGCAG	1 cut(s)	1831
PstNI	CAGNNNCTG	1 cut(s)	1802
PsuI	RGATCY	9 cut(s)	154, 442, 536, 645, 672, 958, 1699, 2626, 2724
RsaI	GTAC	7 cut(s)	664, 941, 1029, 1793, 2213, 2438, 2834
RsaNI	GTAC	7 cut(s)	663, 940, 1028, 1792, 2212, 2437, 2833
RseI	CAYNNNNRTG	4 cut(s)	1146, 1380, 1595, 1911
SacI	GAGCTC	2 cut(s)	1635, 2046
SaqAI	TTAA	8 cut(s)	248, 480, 575, 801, 1310, 2397, 2409, 2905
Sau96I	GGNCC	8 cut(s)	259, 331, 502, 1038, 1496, 1940, 1941, 2574
ScaI	AGTACT	1 cut(s)	2213
SchI	GAGTC	2 cut(s)	2309, 2655
ScrFI	CCNGG	5 cut(s)	264, 1277, 2332, 2505, 2841
SduI	GDGCHC	6 cut(s)	1182, 1635, 1944, 2046, 2094, 2137
SfaNI	GCATC	6 cut(s)	91, 463, 1042, 1381, 1414, 1801
SfcI	CTRYAG	2 cut(s)	1827, 2163
SinI	GGWCC	5 cut(s)	259, 331, 502, 1038, 2574
SmiMI	CAYNNNNRTG	4 cut(s)	1146, 1380, 1595, 1911
SmlI	CTYRAG	2 cut(s)	425, 1309
SmoI	CTYRAG	2 cut(s)	425, 1309
Sse9I	AATT	8 cut(s)	388, 568, 738, 1070, 1281, 1413, 1521, 1880
SsiI	CCGC	7 cut(s)	346, 994, 1235, 2076, 2645, 2665, 2755
SspI	AATATT	1 cut(s)	793
SspMI	CTAG	2 cut(s)	918, 2286
SstI	GAGCTC	2 cut(s)	1635, 2046
StyD4I	CCNGG	5 cut(s)	262, 1275, 2330, 2503, 2839
StyI	CCWWGG	4 cut(s)	1906, 2008, 2318, 2493
TaaI	ACNGT	9 cut(s)	169, 493, 1106, 1473, 1491, 1683, 1924, 2005, 2574
TaiI	ACGT	6 cut(s)	769, 1150, 1156, 1645, 1872, 2748
TaqI	TCGA	5 cut(s)	1239, 1350, 1739, 2208, 2634
TasI	AATT	8 cut(s)	388, 568, 738, 1070, 1281, 1413, 1521, 1880
TatI	WGTACW	4 cut(s)	939, 1027, 2211, 2436
TauI	GCSGC	1 cut(s)	2758
Tru1I	TTAA	8 cut(s)	248, 480, 575, 801, 1310, 2397, 2409, 2905
Tru9I	TTAA	8 cut(s)	248, 480, 575, 801, 1310, 2397, 2409, 2905
TseFI	GTSAC	6 cut(s)	371, 1271, 1999, 2461, 2639, 2746
Tsp45I	GTSAC	6 cut(s)	371, 1271, 1999, 2461, 2639, 2746
TspGWI	ACGGA	7 cut(s)	1001, 1529, 1985, 2109, 2207, 2717, 2737
Van91I	CCANNNNNTGG	1 cut(s)	1493
Vha464I	CTTAAG	1 cut(s)	1309
VneI	GTGCAC	1 cut(s)	2133
VpaK11BI	GGWCC	5 cut(s)	259, 331, 502, 1038, 2574
XagI	CCTNNNNNAGG	1 cut(s)	2289
XapI	RAATTY	4 cut(s)	388, 1070, 1521, 1880
XceI	RCATGY	5 cut(s)	180, 304, 1550, 2393, 2425
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1913, 2124
XmiI	GTMKAC	1 cut(s)	1322
XmnI	GAANNNNTTC	2 cut(s)	168, 810
XspI	CTAG	2 cut(s)	918, 2286
ZrmI	AGTACT	1 cut(s)	2213
Zsp2I	ATGCAT	1 cut(s)	2103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.