Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004914

Physical Location & Seq

Genomic Coordinates Forward (+)

25783339 .. 25790256

Gene Structure 6918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G019770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCTAATAGGAGTTTGGAGAAGTTGGCGTCTATAGACGCTCAGCTTCGGGCTCTGGTTCCGGCGAAAGTGAGTGAGGATGACAAGCTTGTTGAGTATGATGCTCTGCTTTTGGATCGGTTTCTGGATATTCTCCAGGATTTGCATGGAGAAGATCTCAGGGAAACGGTCCAATCGTGTTATGAGCTTTCTGCTGAATATGAAGGAAAGCATGATCCGCAAAAACTGTCAGAACTTGGAAATGTATTGACAAGTTTGGATCCAGGGGATTCCATTGTTATTGCAAAATCCTTCGCTCACATGCTTAGCTTGGCCAACTTGGCTGAGGAGGTCCAGATTGCTTACCGCAGACGAAACAAACTGAAAAAGGGAGACTTTGCAGATGAAAATTCCGCAACAACTGAGTCAGACATTGAAGAAACTCTCAAGAGGCTTGTGGGGGAATTTAAGAAGTCTCCAGAAGAGGTTTTTGATGCTCTGAAAAATCAGACTGTAGATCTTGTCTTCACTGCTCATCCTACACAATCAGTCCGTAGATCCTTGCTTCAGAAGCATGGAAGGATACGGAATTGCTTAACCCAGTTGTATGCCAAAGACATTACTCCAGATGATAAGCAGGAAATTGATGAGGCACTTCAGAGAGAGATTCAAGCTGCATTTCGTACGGATGAGATCAGAAGAACAGCTCCGACTCCACAAGATGAGATGAGAGCAGGGATGAGCTACTTTCATGAAACAATCTGGAATGGTGTCCCAAAGTTTCTGCGTCGTGTTGACACAGCTTTGAAGAACATTGGGATTAATGAACGTGTTCCCTACAATGCCCCACTTATTCAATTCTCATCTTGGATGGGTGGTGATCGCGATGGTAATCCAAGAGTAACTCCTGAAGTCACAAGGGATGTGTGCCTTCTGGCTAGAATGATGGCTGCTAACTTGTACTATTCTCAGATAGAGGATCTTATGTTTGAGTTGTCTATGTGGCGCTGCAATGAGGAACTCCGTGTTCGCGCAGATGTACTTCACAGGTCCTCAAGGAGAGATGCAAAGCACTACATAGAATTTTGGAAGCAAGTTCCTCCCAACGAACCTTACCGTGTGATTCTTGGTGATATAAGGGATAAGCTTTATCATACACGTGAACGTTCTCGTCAGTTGTTAGCCTGTGGGCACTCTGACATTCCAGAGGAAGCAGTATTGACCAGTGTTGAGGAGTTCTTGGAACCACTCGAACTATGCTATAGATCACTCTGCTCTTGTGGAGATCGGGCAATTGCCGATGGCTCCCTTCTTGATTTCTTAAGGCAAGTTTCTACCTTTGGACTTTCACTTGTGAGACTTGATATTCGACAAGAATCTGACAGGCACACTGATGTCATCGATGCCATCACAAAGCATCTGGAAATTGGTTCCTATAGAGAATGGTCCGAAGAACGCCGTCAGCAGTGGCTTCTATCTGAACTTAGTGGCAAGCGCCCGCTTTTTGGCCCTGATCTTCCAAAAACCGAAGAAATTGCTGATGTTTTGGATACATTACATGTGATATCAGAACTCCCATCTGACAACTTTGGAGCATACATCATCTCAATGGCAACTGCTGCATCTGATGTCCTTGCAGTTGAACTCCTGCAACGTGAATGTCATGTGAAGAAACCACTGAGAGTCGTTCCACTGTTTGAGAAGCTTGCAGATTTGGAGGCTGCTCCTGCTGCTTTGGCTCGGCTCTTCTCAATTGATTGGTATAGGGAAAGAATCAATGGAAAGCAAGAAGTCATGATTGGGTACTCAGATTCTGGTAAAGATGCAGGGCGTTTTTCAGCTGGCTGGCAGTTATACAAGGCACAGGAGGACCTTATAAAGGTTGCTAAGGATTTTGGTGTGAAGCTAACTATGTTCCATGGTCGTGGTGGGACTGTTGGAAGGGGAGGTGGGCCCACCCATCTTGCTATATTGTCTCAACCACCTGATACTATTCATGGTTCCCTCCGTGTCACTGTCCAAGGTGAAGTTATTGAGCAGTCTTTTGGAGAGGAGCTCTTGTGTTTCAGAACACTCCAGCGTTTCACAGCTGCTACACTAGAGCATGGAATGCATTTCCCAGTTTCACCAAAACCAGAATGGCGTAAACTGATGGATGAAATGGCTGTTGTATCTACAGAGGCCTATCGTTCTATTGTTTTCAAAGAACCCAGATTCGTCGAGTATTTCCGTCTTGCTACACCAGAGCTGGAGTATGGTCGGATGAACATTGGAAGTCGCCCGTCAAAGCGAAAGCCTAGTGGAGGCATTGAGTCACTTCGTGCAATCCCATGGATTTTTGCCTGGACACAAACGAGGTTCCATCTTCCTGTGTGGTTAGGCTTTGGAGAAGCTTTCAAATATGTCATTGCGAAGGACATTAGGAACCTTCACATGCTGCAGGATATGTACAATCAATGGCCGTTCTTTAGGGTCACCATTGATTTGGTTGAAATGGTGTTTGCCAAGGGAAACCCCGGAATTGCTGCTTTGTATGATAAACTCCTTGTTTCAGAAGACCTATGGTCATTCGGAGAGCGATTGAGAGCCGACTATGCAGAAACCCAGAAATATCTCTTGCAGATTGCTGGACACAAGGATCTTCTTGAAGGGGACCCCCACCTGAAGCAAAGACTCCGACTGCGTGATTCATACATCACCACCCTCAATGTTTGCCAAGCATATACACTGAAAAGGATCCGAGATCCTACCTACCATGTGACTGTGCGACCACATCTGTCCAAGGAGATTTCGGAGTCGAACAAACCAGCTGATGAACTTGTGAAGCTGAACCCAACAAGTGAGTACGCCCCTGGTTTGGAGGACACCCTTATCTTGACAATGAAGGGTATTGCAGCTGGCTTGCAGAACACTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008964 GO:0009267 GO:0009507 GO:0009536 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0051716 GO:0065003 GO:0071496 GO:0071840 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

965

Amino Acids

110.12

Weight (kDa)

5.86

Isoelectric Point (pI)

47.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 965	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53310 AT1G53310 AT1G53310 AT3G14940 AT3G14940
fragaria_vesca	FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430
malus_domestica	MD03G1242000.v1.1 MD11G1261900.v1.1
prunus_persica	Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1
pyrus_communis	pycom03g18910 pycom11g23090
rosa_chinensis	RchiOBHm_Chr5g0031161
rosa_laevigata	RLG00000033287
rosa_multiflora	Rmu_sc0011257.1_g000003
rosa_roxburghii	Rroxscaffold_1G00049100
rosa_rugosa	Rorug05G0122600 Rorug05G0122700
rosa_samantha	Rh5AG216300 Rh5BG215600 Rh5CG239100 Rh5DG220500
rosa_wichuraiana	Rw5G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1856
AccII	CGCG	2 cut(s)	864, 1011
AciI	CCGC	4 cut(s)	218, 346, 393, 1478
AclI	AACGTT	1 cut(s)	1144
AcoI	YGGCCR	2 cut(s)	312, 2438
AcsI	RAATTY	3 cut(s)	388, 443, 1061
AcuI	CTGAAG	4 cut(s)	530, 620, 909, 2663
AcvI	CACGTG	1 cut(s)	1139
AcyI	GRCGYC	1 cut(s)	29
AdeI	CACNNNGTG	1 cut(s)	2300
AfaI	GTAC	6 cut(s)	664, 941, 1020, 1784, 2429, 2825
AfiI	CCNNNNNNNGG	4 cut(s)	1484, 1858, 2282, 2836
AflII	CTTAAG	1 cut(s)	1300
AflIII	ACRYGT	3 cut(s)	808, 1136, 1537
AgsI	TTSAA	9 cut(s)	416, 650, 787, 836, 1622, 2182, 2377, 2471, 2627
AhdI	GACNNNNNGTC	1 cut(s)	2542
AjnI	CCWGG	4 cut(s)	135, 262, 2321, 2830
AjuI	GAANNNNNNNTTGG	2 cut(s)	1761, 1793
Alw21I	GWGCWC	1 cut(s)	2037
Alw26I	GTCTC	4 cut(s)	366, 459, 1330, 1959
AlwNI	CAGNNNCTG	1 cut(s)	1793
AoxI	GGCC	5 cut(s)	312, 1486, 1931, 2160, 2438
ApaI	GGGCCC	1 cut(s)	1935
ApoI	RAATTY	3 cut(s)	388, 443, 1061
ArsI	GACNNNNNNTTYG	2 cut(s)	1329, 1361
AseI	ATTAAT	1 cut(s)	801
Asp700I	GAANNNNTTC	1 cut(s)	810
AspLEI	GCGC	3 cut(s)	987, 1013, 1476
AspS9I	GGNCC	9 cut(s)	169, 331, 1029, 1425, 1487, 1849, 1931, 1932, 2632
AsuC2I	CCSGG	1 cut(s)	2496
AsuHPI	GGTGA	6 cut(s)	869, 1121, 2015, 2097, 2446, 2668
AvaII	GGWCC	6 cut(s)	169, 331, 1029, 1425, 1849, 2632
BaeGI	GKGCMC	2 cut(s)	1173, 1935
BalI	TGGCCA	1 cut(s)	314
BamHI	GGATCC	2 cut(s)	259, 2715
BanII	GRGCYC	3 cut(s)	55, 1935, 2037
BbrPI	CACGTG	1 cut(s)	1139
BbsI	GAAGAC	2 cut(s)	496, 2541
Bbv12I	GWGCWC	1 cut(s)	2037
BbvCI	CCTCAGC	1 cut(s)	324
BccI	CCATC	9 cut(s)	844, 860, 919, 1274, 1394, 1564, 1947, 2125, 2349
BceAI	ACGGC	2 cut(s)	1422, 2425
BcgI	CGANNNNNNTGC	2 cut(s)	1496, 1530
BciT130I	CCWGG	4 cut(s)	137, 264, 2323, 2832
BciVI	GTATCC	2 cut(s)	555, 1522
BcnI	CCSGG	1 cut(s)	2496
BcoDI	GTCTC	4 cut(s)	366, 459, 1330, 1959
BfaI	CTAG	3 cut(s)	918, 2078, 2277
BfmI	CTRYAG	6 cut(s)	33, 492, 1240, 1414, 2154, 2417
BfoI	RGCGCY	2 cut(s)	988, 1477
BfrI	CTTAAG	1 cut(s)	1300
BfuI	GTATCC	2 cut(s)	555, 1522
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	2 cut(s)	154, 496
BlpI	GCTNAGC	2 cut(s)	42, 305
Bme1390I	CCNGG	5 cut(s)	137, 264, 2323, 2496, 2832
Bme18I	GGWCC	6 cut(s)	169, 331, 1029, 1425, 1849, 2632
BmeRI	GACNNNNNGTC	1 cut(s)	2542
BmgT120I	GGNCC	9 cut(s)	169, 331, 1029, 1425, 1487, 1849, 1931, 1932, 2632
BmrFI	CCNGG	5 cut(s)	137, 264, 2323, 2496, 2832
BmrI	ACTGGG	2 cut(s)	574, 2093
BmsI	GCATC	7 cut(s)	91, 463, 1033, 1372, 1405, 1610, 1792
BmuI	ACTGGG	2 cut(s)	574, 2093
BpiI	GAAGAC	2 cut(s)	496, 2541
BplI	GAGNNNNNCTC	4 cut(s)	141, 173, 2019, 2051
BpmI	CTGGAG	5 cut(s)	119, 441, 588, 2039, 2249
Bpu10I	CCTNAGC	2 cut(s)	324, 1866
Bpu1102I	GCTNAGC	2 cut(s)	42, 305
BpuEI	CTTGAG	2 cut(s)	410, 1018
BpuMI	CCSGG	1 cut(s)	2496
Bsa29I	ATCGAT	1 cut(s)	1380
BsaAI	YACGTR	1 cut(s)	1139
BsaBI	GATNNNNATC	1 cut(s)	870
BsaHI	GRCGYC	1 cut(s)	29
BsaJI	CCNNGG	8 cut(s)	263, 1897, 1999, 2309, 2484, 2494, 2760, 2830
BsaXI	ACNNNNNCTCC	2 cut(s)	2024, 2054
Bsc4I	CCNNNNNNNGG	4 cut(s)	1484, 1858, 2282, 2836
Bse1I	ACTGG	4 cut(s)	580, 1203, 2099, 2896
Bse3DI	GCAATG	2 cut(s)	997, 2385
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	4 cut(s)	137, 264, 2323, 2832
BseCI	ATCGAT	1 cut(s)	1380
BseDI	CCNNGG	8 cut(s)	263, 1897, 1999, 2309, 2484, 2494, 2760, 2830
BseGI	GGATG	8 cut(s)	85, 514, 673, 723, 855, 907, 2140, 2247
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	4 cut(s)	1484, 1858, 2282, 2836
BseMI	GCAATG	2 cut(s)	997, 2385
BseMII	CTCAG	7 cut(s)	56, 172, 315, 393, 962, 1649, 1800
BseNI	ACTGG	4 cut(s)	580, 1203, 2099, 2896
BseRI	GAGGAG	3 cut(s)	341, 1226, 2045
BseSI	GKGCMC	2 cut(s)	1173, 1935
Bsh1236I	CGCG	2 cut(s)	864, 1011
BshFI	GGCC	5 cut(s)	314, 1488, 1933, 2162, 2440
BshVI	ATCGAT	1 cut(s)	1380
BsiHKAI	GWGCWC	1 cut(s)	2037
BsiSI	CCGG	2 cut(s)	62, 2496
BsiWI	CGTACG	1 cut(s)	662
BslFI	GGGAC	3 cut(s)	737, 1924, 2645
BslI	CCNNNNNNNGG	4 cut(s)	1484, 1858, 2282, 2836
BsmAI	GTCTC	4 cut(s)	366, 459, 1330, 1959
BsmFI	GGGAC	3 cut(s)	737, 1924, 2645
BsmI	GAATGC	1 cut(s)	2094
BsnI	GGCC	5 cut(s)	314, 1488, 1933, 2162, 2440
Bsp120I	GGGCCC	1 cut(s)	1931
Bsp1286I	GDGCHC	4 cut(s)	55, 1173, 1935, 2037
Bsp1407I	TGTACA	1 cut(s)	2427
Bsp1720I	GCTNAGC	2 cut(s)	42, 305
Bsp19I	CCATGG	2 cut(s)	1897, 2309
Bsp68I	TCGCGA	1 cut(s)	864
BspACI	CCGC	4 cut(s)	218, 346, 393, 1478
BspANI	GGCC	5 cut(s)	314, 1488, 1933, 2162, 2440
BspCNI	CTCAG	7 cut(s)	55, 171, 316, 394, 961, 1650, 1799
BspDI	ATCGAT	1 cut(s)	1380
BspFNI	CGCG	2 cut(s)	864, 1011
BspHI	TCATGA	2 cut(s)	730, 1773
BspMAI	CTGCAG	1 cut(s)	2421
BspQI	GCTCTTC	1 cut(s)	1730
BspTI	CTTAAG	1 cut(s)	1300
BsrDI	GCAATG	2 cut(s)	997, 2385
BsrGI	TGTACA	1 cut(s)	2427
BsrI	ACTGG	4 cut(s)	580, 1203, 2099, 2896
BssECI	CCNNGG	8 cut(s)	263, 1897, 1999, 2309, 2484, 2494, 2760, 2830
BssNI	GRCGYC	1 cut(s)	29
BssT1I	CCWWGG	5 cut(s)	1897, 1999, 2309, 2484, 2760
Bst2UI	CCWGG	4 cut(s)	137, 264, 2323, 2832
Bst4CI	ACNGT	8 cut(s)	169, 228, 493, 1097, 1674, 1915, 1996, 2743
Bst6I	CTCTTC	2 cut(s)	456, 1730
BstACI	GRCGYC	1 cut(s)	29
BstAFI	CTTAAG	1 cut(s)	1300
BstAPI	GCANNNNNTGC	2 cut(s)	1598, 2089
BstAUI	TGTACA	1 cut(s)	2427
BstBAI	YACGTR	1 cut(s)	1139
BstC8I	GCNNGC	7 cut(s)	1472, 1478, 1686, 1822, 1826, 2878, 2882
BstDSI	CCRYGG	2 cut(s)	1897, 2309
BstEII	GGTNACC	1 cut(s)	2452
BstENI	CCTNNNNNAGG	2 cut(s)	1856, 2280
BstF5I	GGATG	8 cut(s)	85, 514, 673, 723, 855, 907, 2140, 2247
BstFNI	CGCG	2 cut(s)	864, 1011
BstH2I	RGCGCY	2 cut(s)	988, 1477
BstHHI	GCGC	3 cut(s)	987, 1013, 1476
BstMAI	GTCTC	4 cut(s)	366, 459, 1330, 1959
BstMWI	GCNNNNNNNGC	9 cut(s)	217, 320, 550, 1598, 1706, 1709, 1715, 2089, 2573
BstNI	CCWGG	4 cut(s)	137, 264, 2323, 2832
BstNSI	RCATGY	3 cut(s)	304, 1541, 2416
BstPI	GGTNACC	1 cut(s)	2452
BstSCI	CCNGG	5 cut(s)	135, 262, 2321, 2494, 2830
BstSFI	CTRYAG	6 cut(s)	33, 492, 1240, 1414, 2154, 2417
BstSLI	GKGCMC	2 cut(s)	1173, 1935
BstUI	CGCG	2 cut(s)	864, 1011
BstV2I	GAAGAC	2 cut(s)	496, 2541
BstX2I	RGATCY	8 cut(s)	154, 259, 496, 536, 958, 2617, 2715, 2722
BstXI	CCANNNNNNTGG	2 cut(s)	1904, 2464
BstYI	RGATCY	8 cut(s)	154, 259, 496, 536, 958, 2617, 2715, 2722
Bsu15I	ATCGAT	1 cut(s)	1380
BsuI	GTATCC	2 cut(s)	555, 1522
BsuRI	GGCC	5 cut(s)	314, 1488, 1933, 2162, 2440
BsuTUI	ATCGAT	1 cut(s)	1380
BtgI	CCRYGG	2 cut(s)	1897, 2309
BtgZI	GCGATG	1 cut(s)	879
BtsCI	GGATG	8 cut(s)	85, 514, 673, 723, 855, 907, 2140, 2247
BtsI	GCAGTG	2 cut(s)	507, 1451
BtsIMutI	CAGTG	9 cut(s)	507, 1210, 1368, 1451, 1655, 1670, 1992, 2705, 2889
BtuMI	TCGCGA	1 cut(s)	864
Cac8I	GCNNGC	7 cut(s)	1472, 1478, 1686, 1822, 1826, 2878, 2882
CaiI	CAGNNNCTG	1 cut(s)	1793
CciI	TCATGA	2 cut(s)	730, 1773
CfoI	GCGC	3 cut(s)	987, 1013, 1476
Cfr13I	GGNCC	9 cut(s)	169, 331, 1029, 1425, 1487, 1849, 1931, 1932, 2632
ClaI	ATCGAT	1 cut(s)	1380
CseI	GACGC	3 cut(s)	18, 47, 755
Csp6I	GTAC	6 cut(s)	663, 940, 1019, 1783, 2428, 2824
CspCI	CAANNNNNGTGG	2 cut(s)	1924, 1959
CviQI	GTAC	6 cut(s)	663, 940, 1019, 1783, 2428, 2824
DraIII	CACNNNGTG	1 cut(s)	2300
DriI	GACNNNNNGTC	1 cut(s)	2542
EaeI	YGGCCR	2 cut(s)	312, 2438
Eam1104I	CTCTTC	2 cut(s)	456, 1730
Eam1105I	GACNNNNNGTC	1 cut(s)	2542
EarI	CTCTTC	2 cut(s)	456, 1730
Ecl136II	GAGCTC	1 cut(s)	2035
Eco130I	CCWWGG	5 cut(s)	1897, 1999, 2309, 2484, 2760
Eco147I	AGGCCT	1 cut(s)	2162
Eco24I	GRGCYC	3 cut(s)	55, 1935, 2037
Eco32I	GATATC	1 cut(s)	1545
Eco47I	GGWCC	6 cut(s)	169, 331, 1029, 1425, 1849, 2632
Eco53kI	GAGCTC	1 cut(s)	2035
Eco57I	CTGAAG	4 cut(s)	530, 620, 909, 2663
Eco72I	CACGTG	1 cut(s)	1139
Eco91I	GGTNACC	1 cut(s)	2452
EcoICRI	GAGCTC	1 cut(s)	2035
EcoNI	CCTNNNNNAGG	2 cut(s)	1856, 2280
EcoO109I	RGGNCCY	3 cut(s)	1029, 1849, 2632
EcoO65I	GGTNACC	1 cut(s)	2452
EcoRII	CCWGG	4 cut(s)	135, 262, 2321, 2830
EcoRV	GATATC	1 cut(s)	1545
EcoT14I	CCWWGG	5 cut(s)	1897, 1999, 2309, 2484, 2760
EcoT22I	ATGCAT	1 cut(s)	2094
EcoT38I	GRGCYC	3 cut(s)	55, 1935, 2037
ErhI	CCWWGG	5 cut(s)	1897, 1999, 2309, 2484, 2760
FalI	AAGNNNNNCTT	2 cut(s)	359, 391
FaqI	GGGAC	3 cut(s)	737, 1924, 2645
FauI	CCCGC	1 cut(s)	1485
FokI	GGATG	8 cut(s)	92, 501, 680, 730, 862, 914, 2147, 2254
FriOI	GRGCYC	3 cut(s)	55, 1935, 2037
FspBI	CTAG	3 cut(s)	918, 2078, 2277
GlaI	GCGC	3 cut(s)	986, 1012, 1475
GsuI	CTGGAG	5 cut(s)	119, 441, 588, 2039, 2249
HaeII	RGCGCY	2 cut(s)	988, 1477
HaeIII	GGCC	5 cut(s)	314, 1488, 1933, 2162, 2440
HapII	CCGG	2 cut(s)	62, 2496
HgaI	GACGC	3 cut(s)	18, 47, 755
HhaI	GCGC	3 cut(s)	987, 1013, 1476
Hin1I	GRCGYC	1 cut(s)	29
Hin6I	GCGC	3 cut(s)	985, 1011, 1474
HinP1I	GCGC	3 cut(s)	985, 1011, 1474
HincII	GTYRAC	1 cut(s)	775
HindII	GTYRAC	1 cut(s)	775
HindIII	AAGCTT	4 cut(s)	86, 1124, 1682, 2370
HpaII	CCGG	2 cut(s)	62, 2496
HphI	GGTGA	6 cut(s)	869, 1121, 2015, 2097, 2446, 2668
Hpy166II	GTNNAC	3 cut(s)	775, 1142, 2126
Hpy8I	GTNNAC	3 cut(s)	775, 1142, 2126
Hpy99I	CGWCG	2 cut(s)	771, 2201
HpyCH4III	ACNGT	8 cut(s)	169, 228, 493, 1097, 1674, 1915, 1996, 2743
HpyCH4IV	ACGT	4 cut(s)	808, 1138, 1144, 1633
HpyF10VI	GCNNNNNNNGC	9 cut(s)	217, 320, 550, 1598, 1706, 1709, 1715, 2089, 2573
HpySE526I	ACGT	4 cut(s)	808, 1138, 1144, 1633
Hsp92I	GRCGYC	1 cut(s)	29
HspAI	GCGC	3 cut(s)	985, 1011, 1474
KflI	GGGWCCC	1 cut(s)	2632
LguI	GCTCTTC	1 cut(s)	1730
LmnI	GCTCC	5 cut(s)	691, 1289, 1571, 1708, 2032
LweI	GCATC	7 cut(s)	91, 463, 1033, 1372, 1405, 1610, 1792
MaeI	CTAG	3 cut(s)	918, 2078, 2277
MaeII	ACGT	4 cut(s)	808, 1138, 1144, 1633
MaeIII	GTNAC	6 cut(s)	880, 892, 1990, 2292, 2452, 2737
MfeI	CAATTG	2 cut(s)	1272, 1731
MflI	RGATCY	8 cut(s)	154, 259, 496, 536, 958, 2617, 2715, 2722
MhlI	GDGCHC	4 cut(s)	55, 1173, 1935, 2037
MlsI	TGGCCA	1 cut(s)	314
MluNI	TGGCCA	1 cut(s)	314
MlyI	GAGTC	6 cut(s)	413, 685, 1671, 2300, 2646, 2783
MmeI	TCCRAC	4 cut(s)	713, 1897, 2219, 2680
Mox20I	TGGCCA	1 cut(s)	314
Mph1103I	ATGCAT	1 cut(s)	2094
MroXI	GAANNNNTTC	1 cut(s)	810
MscI	TGGCCA	1 cut(s)	314
MseI	TTAA	5 cut(s)	447, 575, 801, 1301, 2896
MslI	CAYNNNNRTG	4 cut(s)	1137, 1371, 1586, 1902
Msp20I	TGGCCA	1 cut(s)	314
MspA1I	CMGCKG	4 cut(s)	1820, 2069, 2789, 2876
MspCI	CTTAAG	1 cut(s)	1300
MspI	CCGG	2 cut(s)	62, 2496
MspR9I	CCNGG	5 cut(s)	137, 264, 2323, 2496, 2832
MunI	CAATTG	2 cut(s)	1272, 1731
Mva1269I	GAATGC	1 cut(s)	2094
MvaI	CCWGG	4 cut(s)	137, 264, 2323, 2832
MvnI	CGCG	2 cut(s)	864, 1011
MwoI	GCNNNNNNNGC	9 cut(s)	217, 320, 550, 1598, 1706, 1709, 1715, 2089, 2573
NciI	CCSGG	1 cut(s)	2496
NcoI	CCATGG	2 cut(s)	1897, 2309
NmeAIII	GCCGAG	1 cut(s)	1699
NmuCI	GTSAC	5 cut(s)	892, 1990, 2292, 2452, 2737
NruI	TCGCGA	1 cut(s)	864
NsiI	ATGCAT	1 cut(s)	2094
NspI	RCATGY	3 cut(s)	304, 1541, 2416
PagI	TCATGA	2 cut(s)	730, 1773
PceI	AGGCCT	1 cut(s)	2162
PciI	ACATGT	1 cut(s)	1537
PciSI	GCTCTTC	1 cut(s)	1730
PcsI	WCGNNNNNNNCGW	1 cut(s)	173
PctI	GAATGC	1 cut(s)	2094
PdmI	GAANNNNTTC	1 cut(s)	810
PfeI	GAWTC	8 cut(s)	269, 646, 1102, 1355, 1752, 1790, 2193, 2666
Pfl23II	CGTACG	1 cut(s)	662
PfoI	TCCNGGA	1 cut(s)	135
PleI	GAGTC	6 cut(s)	412, 685, 1670, 2299, 2646, 2782
PmaCI	CACGTG	1 cut(s)	1139
PmlI	CACGTG	1 cut(s)	1139
PpsI	GAGTC	6 cut(s)	412, 685, 1670, 2299, 2646, 2782
Ppu21I	YACGTR	1 cut(s)	1139
PpuMI	RGGWCCY	3 cut(s)	1029, 1849, 2632
PscI	ACATGT	1 cut(s)	1537
PshBI	ATTAAT	1 cut(s)	801
PsiI	TTATAA	1 cut(s)	1856
Psp124BI	GAGCTC	1 cut(s)	2037
Psp1406I	AACGTT	1 cut(s)	1144
Psp5II	RGGWCCY	3 cut(s)	1029, 1849, 2632
Psp6I	CCWGG	4 cut(s)	135, 262, 2321, 2830
PspCI	CACGTG	1 cut(s)	1139
PspEI	GGTNACC	1 cut(s)	2452
PspGI	CCWGG	4 cut(s)	135, 262, 2321, 2830
PspLI	CGTACG	1 cut(s)	662
PspOMI	GGGCCC	1 cut(s)	1931
PspPI	GGNCC	9 cut(s)	169, 331, 1029, 1425, 1487, 1849, 1931, 1932, 2632
PspPPI	RGGWCCY	3 cut(s)	1029, 1849, 2632
PstI	CTGCAG	1 cut(s)	2421
PstNI	CAGNNNCTG	1 cut(s)	1793
PsuI	RGATCY	8 cut(s)	154, 259, 496, 536, 958, 2617, 2715, 2722
PvuII	CAGCTG	4 cut(s)	1820, 2069, 2789, 2876
RruI	TCGCGA	1 cut(s)	864
RsaI	GTAC	6 cut(s)	664, 941, 1020, 1784, 2429, 2825
RsaNI	GTAC	6 cut(s)	663, 940, 1019, 1783, 2428, 2824
RseI	CAYNNNNRTG	4 cut(s)	1137, 1371, 1586, 1902
SacI	GAGCTC	1 cut(s)	2037
SapI	GCTCTTC	1 cut(s)	1730
SaqAI	TTAA	5 cut(s)	447, 575, 801, 1301, 2896
Sau96I	GGNCC	9 cut(s)	169, 331, 1029, 1425, 1487, 1849, 1931, 1932, 2632
SchI	GAGTC	6 cut(s)	413, 685, 1671, 2300, 2646, 2783
ScrFI	CCNGG	5 cut(s)	137, 264, 2323, 2496, 2832
SduI	GDGCHC	4 cut(s)	55, 1173, 1935, 2037
SfaNI	GCATC	7 cut(s)	91, 463, 1033, 1372, 1405, 1610, 1792
SfcI	CTRYAG	6 cut(s)	33, 492, 1240, 1414, 2154, 2417
SinI	GGWCC	6 cut(s)	169, 331, 1029, 1425, 1849, 2632
SmiMI	CAYNNNNRTG	4 cut(s)	1137, 1371, 1586, 1902
SmlI	CTYRAG	3 cut(s)	425, 1033, 1300
SmoI	CTYRAG	3 cut(s)	425, 1033, 1300
SseBI	AGGCCT	1 cut(s)	2162
SsiI	CCGC	4 cut(s)	218, 346, 393, 1478
SspMI	CTAG	3 cut(s)	918, 2078, 2277
SstI	GAGCTC	1 cut(s)	2037
StuI	AGGCCT	1 cut(s)	2162
StyD4I	CCNGG	5 cut(s)	135, 262, 2321, 2494, 2830
StyI	CCWWGG	5 cut(s)	1897, 1999, 2309, 2484, 2760
TaaI	ACNGT	8 cut(s)	169, 228, 493, 1097, 1674, 1915, 1996, 2743
TaiI	ACGT	4 cut(s)	811, 1141, 1147, 1636
TaqI	TCGA	5 cut(s)	1230, 1348, 1380, 2199, 2777
TatI	WGTACW	3 cut(s)	939, 1018, 2427
TfiI	GAWTC	8 cut(s)	269, 646, 1102, 1355, 1752, 1790, 2193, 2666
Tru1I	TTAA	5 cut(s)	447, 575, 801, 1301, 2896
Tru9I	TTAA	5 cut(s)	447, 575, 801, 1301, 2896
TscAI	CASTG	9 cut(s)	514, 1210, 1375, 1451, 1662, 1677, 1999, 2712, 2896
TseFI	GTSAC	5 cut(s)	892, 1990, 2292, 2452, 2737
Tsp45I	GTSAC	5 cut(s)	892, 1990, 2292, 2452, 2737
TspGWI	ACGGA	6 cut(s)	521, 580, 680, 992, 1976, 2198
TspRI	CASTG	9 cut(s)	514, 1210, 1375, 1451, 1662, 1677, 1999, 2712, 2896
Vha464I	CTTAAG	1 cut(s)	1300
VpaK11BI	GGWCC	6 cut(s)	169, 331, 1029, 1425, 1849, 2632
VspI	ATTAAT	1 cut(s)	801
XagI	CCTNNNNNAGG	2 cut(s)	1856, 2280
XapI	RAATTY	3 cut(s)	388, 443, 1061
XceI	RCATGY	3 cut(s)	304, 1541, 2416
XcmI	CCANNNNNNNNNTGG	3 cut(s)	143, 1904, 2115
XmnI	GAANNNNTTC	1 cut(s)	810
XspI	CTAG	3 cut(s)	918, 2078, 2277
Zsp2I	ATGCAT	1 cut(s)	2094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.