Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0004914

Physical Location & Seq

Genomic Coordinates Forward (+)

24819157 .. 24825427

Gene Structure 6271 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG220500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGCTTAGCTTGGCCAACTTGGCTGAGGAGGTCCAGATTGCTTACCGCAGACGAAACAAACTGAAAAAGGGAGACTTTGCAGATGAAAATTCCGCAACAACTGAGTCAGACATTGAAGAAACTCTCAAGAGGCTTGTGGGGGAATTTAAGAAGTCTCCGGAAGAGGTTTTTGATGCTCTGAAAAATCAGACTGTAGATCTTGTCTTCACTGCTCATCCTACACAATCAGTCCGTAGATCCTTGCTTCAGAAGCATGGAAGGATACGGAATTGCTTAACCCAGTTGTATGCCAAAGACATTACTCCAGATGATAAGCAGGAAATTGATGAGGCACTTCAGAGAGAGATTCAAGCTGCATTTCGTACGGATGAGATCAGAAGAACAGCTCCGACTCCACAAGATGAGATGAGAGCAGGGATGAGCTACTTTCATGAAACAGTCTGGAATGGTGTCCCAAAGTTTCTGCGTCGTGTTGACACAGCTTTGAAGAACATTGGGATTAATGAACGCGTTCCCTACAATGCCCCACTTATTCAATTCTCTTCTTGGATGGGTGGTGATCGCGATGGTAATCCAAGAGTAACTCCTGAAGTCACAAGGGATGTGTGCCTTCTGGCTAGAATGATGGCTGCTAACTTGTACTATTCTCAGATAGAGGATCTTATGTTTGAGTTGTCTATGTGGCGCTGCAATGAGGAACTCCGTGTTCGTGCAGACGTACTTCACAGGTCCTCAAGGAGAGATGCAAAGCACTACATAGAATTTTGGAAGCAAGTTCCTCCCAACGAACCCTACCGTGTGATTCTTGGTGATATAAGGGATAAGCTTTATCATACACGGGAACGTTCTCGTCAGTTGTTAGCCTGTGGGCACTCTGACATTCCAGAGGAAGCAGTATTGACCAGTGTTGAGGAGTTCTTGGAACCACTCGAACTATGCTATAGATCACTCTGCTCTTGTGGAGATCGGGCAATTGCCGATGGCTCCCTTCTTGATTTCTTAAGGCAAGTTTCTACCTTTGGACTTTCACTTGTGAGACTTGATATTCGGCAAGAATCTGACAGGCACACTGATGTCATCGATGCCATCACAAAGCATCTGGAAATTGGTTCCTATAGAGAATGGTCCGAAGAACGCCGTCAGCAGTGGCTTCTATCTGAACTTAGTGGCAAGCGCCCGCTTTTTGGCCCTGATCTTCCAAAAACCGAAGAAATTGCTGATGTTTTGGATACATTACATGTGATATCAGAACTCCCATCTGACAACTTTGGAGCATACATCATCTCAATGGCAACTGCTCCATCTGATGTCCTTGCAGTTGAACTCCTGCAACGTGAATGTCATGTGAAGAAACCACTGAGAGTCGTTCCACTGTTTGAGAAGCTTGCAGATTTGGAGGCTGCTCCTGCTGCTTTGGCTCGGCTCTTCTCAATTGATTGGTATAGGGAAAGGATCAATGGAAAGCAAGAAGTCATGATTGGGTACTCAGATTCTGGTAAAGATGCAGGGCGTTTTTCAGCTGGCTGGCAGTTATACAAGGCACAGGAGGACCTTATAAAGGTTGCTAAGGATTTTGGTGTGAAGCTAACTATGTTCCATGGTCGTGGTGGGACTGTTGGAAGGGGAGGTGGGCCCACCCATCTTGCTATATTGTCTCAACCACCTGATACTATTCATGGTTCCCTCCGTGTCACTGTCCAAGGTGAAGTTATTGAGCAGTCTTTTGGAGAGGAGCTCTTGTGTTTTAGAACACTCCAGCGTTTCACAGCTGCTACACTAGAGCATGGAATGCATTTCCCAGTTTCACCAAAACCAGAATGGCGTAAACTTATGGATGAAATGGCTGTTGTATCTACAGAGGCCTATCGTTCTATTGTTTTCAAAGAACCCAGATTTGTTGAGTATTTCCGTCTTGCTACACCAGAGCTGGAGTATGGTCGGATGAACATTGGAAGTCGCCCGTCAAAGCGAAAGCCTAGTGGAGGCATTGAGTCACTTCGTGCAATCCCATGGATTTTTGCCTGGACACAAACGAGGTTCCATCTTCCTGTGTGGTTAGGCTTTGGAGAAGCTTTCAAATATGTCATTGCGAAGGACATTAGGAACCTTCACATGCTGCAGGATATGTACAATCAATGGCCGTTCTTTAGGGTCACCATTGATTTGGTTGAAATGGTGTTTGCCAAGGGAAACCCCGGAATTGCTGCTTTGTATGATAAACTCCTTGTTTCAGAAGACCTATGGTCATTCGGAGAGCGATTGAGAGCCGACTATGCAGAAACCCAGAAATATCTCTTGCAGATTGCTGGTCACAAGGATCTTCTTGAAGGGGACCCCCACCTGAAGCAAAGACTCCGACTGCGTGATTCATACATCACCACCCTCAATGTTTGCCAAGCATATACACTGAAAAGGATCCGAGATCCTACCTACCATGTGACTGTGCGACCACATCTGTCCAAGGAGATTACGGAGTCAAACAAACCAGCTGATGAACTTGTGAAGCTGAACCCAACAAGTGAGTACGCCCCTGGTTTGGAGGACACCCTTATCTTGACAATGAAGGGTATTGCAGCTGGCTTGCAGAACACTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008964 GO:0009267 GO:0009507 GO:0009536 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0051716 GO:0065003 GO:0071496 GO:0071840 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

865

Amino Acids

99.04

Weight (kDa)

6.31

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	1 - 865	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53310 AT1G53310 AT1G53310 AT3G14940 AT3G14940
fragaria_vesca	FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430 FvH4_3g18430
malus_domestica	MD03G1242000.v1.1 MD11G1261900.v1.1
prunus_persica	Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1 Prupe.4G166400_v2.0.a1
pyrus_communis	pycom03g18910 pycom11g23090
rosa_chinensis	RchiOBHm_Chr5g0031161
rosa_laevigata	RLG00000033287
rosa_multiflora	Rmu_sc0011257.1_g000003
rosa_roxburghii	Rroxscaffold_1G00049100
rosa_rugosa	Rorug05G0122600 Rorug05G0122700
rosa_samantha	Rh5AG216300 Rh5BG215600 Rh5CG239100 Rh5DG220500
rosa_wichuraiana	Rw5G019770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1556
AccII	CGCG	2 cut(s)	510, 564
AccIII	TCCGGA	1 cut(s)	157
AciI	CCGC	3 cut(s)	46, 93, 1178
AclI	AACGTT	1 cut(s)	844
AclWI	GGATC	7 cut(s)	231, 666, 1460, 2325, 2410, 2417, 2423
AcoI	YGGCCR	2 cut(s)	12, 2138
AcsI	RAATTY	3 cut(s)	88, 143, 761
AcuI	CTGAAG	4 cut(s)	230, 320, 609, 2363
AdeI	CACNNNGTG	1 cut(s)	2000
AfaI	GTAC	6 cut(s)	364, 641, 720, 1484, 2129, 2525
AfiI	CCNNNNNNNGG	4 cut(s)	1184, 1558, 1982, 2536
AflII	CTTAAG	1 cut(s)	1000
AflIII	ACRYGT	2 cut(s)	508, 1237
AgsI	TTSAA	9 cut(s)	116, 350, 487, 536, 1322, 1882, 2077, 2171, 2327
AhdI	GACNNNNNGTC	1 cut(s)	2242
AjnI	CCWGG	2 cut(s)	2021, 2530
AjuI	GAANNNNNNNTTGG	2 cut(s)	1461, 1493
Alw21I	GWGCWC	1 cut(s)	1737
Alw26I	GTCTC	4 cut(s)	66, 159, 1030, 1659
AlwI	GGATC	7 cut(s)	231, 666, 1460, 2325, 2410, 2417, 2423
AlwNI	CAGNNNCTG	1 cut(s)	1493
Aor13HI	TCCGGA	1 cut(s)	157
AoxI	GGCC	5 cut(s)	12, 1186, 1631, 1860, 2138
ApaI	GGGCCC	1 cut(s)	1635
ApeKI	GCWGC	9 cut(s)	353, 629, 687, 1400, 1409, 1769, 2116, 2204, 2573
ApoI	RAATTY	3 cut(s)	88, 143, 761
ArsI	GACNNNNNNTTYG	2 cut(s)	1029, 1061
AseI	ATTAAT	1 cut(s)	501
Asp700I	GAANNNNTTC	1 cut(s)	510
AspLEI	GCGC	2 cut(s)	687, 1176
AspS9I	GGNCC	8 cut(s)	31, 729, 1125, 1187, 1549, 1631, 1632, 2332
AsuC2I	CCSGG	1 cut(s)	2196
AsuHPI	GGTGA	6 cut(s)	569, 821, 1715, 1797, 2146, 2368
AvaII	GGWCC	5 cut(s)	31, 729, 1125, 1549, 2332
BaeGI	GKGCMC	2 cut(s)	873, 1635
BalI	TGGCCA	1 cut(s)	14
BamHI	GGATCC	1 cut(s)	2415
BanII	GRGCYC	2 cut(s)	1635, 1737
BbsI	GAAGAC	2 cut(s)	196, 2241
Bbv12I	GWGCWC	1 cut(s)	1737
BbvCI	CCTCAGC	1 cut(s)	24
BbvI	GCAGC	9 cut(s)	340, 616, 674, 1387, 1396, 1756, 2103, 2191, 2585
BccI	CCATC	9 cut(s)	544, 560, 619, 974, 1094, 1264, 1309, 1647, 2049
BceAI	ACGGC	2 cut(s)	1122, 2125
BcgI	CGANNNNNNTGC	2 cut(s)	1196, 1230
BciT130I	CCWGG	2 cut(s)	2023, 2532
BciVI	GTATCC	2 cut(s)	255, 1222
BcnI	CCSGG	1 cut(s)	2196
BcoDI	GTCTC	4 cut(s)	66, 159, 1030, 1659
BfaI	CTAG	3 cut(s)	618, 1778, 1977
BfmI	CTRYAG	5 cut(s)	192, 940, 1114, 1854, 2117
BfoI	RGCGCY	2 cut(s)	688, 1177
BfrI	CTTAAG	1 cut(s)	1000
BfuI	GTATCC	2 cut(s)	255, 1222
BglI	GCCNNNNNGGC	1 cut(s)	20
BglII	AGATCT	1 cut(s)	196
BisI	GCNGC	9 cut(s)	354, 630, 688, 1401, 1410, 1770, 2117, 2205, 2574
BlpI	GCTNAGC	1 cut(s)	5
BlsI	GCNGC	9 cut(s)	355, 631, 689, 1402, 1411, 1771, 2118, 2206, 2575
Bme1390I	CCNGG	3 cut(s)	2023, 2196, 2532
Bme18I	GGWCC	5 cut(s)	31, 729, 1125, 1549, 2332
BmeRI	GACNNNNNGTC	1 cut(s)	2242
BmgT120I	GGNCC	8 cut(s)	31, 729, 1125, 1187, 1549, 1631, 1632, 2332
BmrFI	CCNGG	3 cut(s)	2023, 2196, 2532
BmrI	ACTGGG	2 cut(s)	274, 1793
BmsI	GCATC	5 cut(s)	163, 733, 1072, 1105, 1492
BmuI	ACTGGG	2 cut(s)	274, 1793
BpiI	GAAGAC	2 cut(s)	196, 2241
BplI	GAGNNNNNCTC	2 cut(s)	1719, 1751
BpmI	CTGGAG	3 cut(s)	288, 1739, 1949
Bpu10I	CCTNAGC	2 cut(s)	24, 1566
Bpu1102I	GCTNAGC	1 cut(s)	5
BpuEI	CTTGAG	2 cut(s)	110, 718
BpuMI	CCSGG	1 cut(s)	2196
Bsa29I	ATCGAT	1 cut(s)	1080
BsaBI	GATNNNNATC	1 cut(s)	570
BsaJI	CCNNGG	7 cut(s)	1597, 1699, 2009, 2184, 2194, 2460, 2530
BsaWI	WCCGGW	1 cut(s)	157
BsaXI	ACNNNNNCTCC	2 cut(s)	1724, 1754
Bsc4I	CCNNNNNNNGG	4 cut(s)	1184, 1558, 1982, 2536
Bse1I	ACTGG	4 cut(s)	280, 903, 1799, 2596
Bse3DI	GCAATG	2 cut(s)	697, 2085
Bse8I	GATNNNNATC	1 cut(s)	570
BseAI	TCCGGA	1 cut(s)	157
BseBI	CCWGG	2 cut(s)	2023, 2532
BseCI	ATCGAT	1 cut(s)	1080
BseDI	CCNNGG	7 cut(s)	1597, 1699, 2009, 2184, 2194, 2460, 2530
BseGI	GGATG	7 cut(s)	214, 373, 423, 555, 607, 1840, 1947
BseJI	GATNNNNATC	1 cut(s)	570
BseLI	CCNNNNNNNGG	4 cut(s)	1184, 1558, 1982, 2536
BseMI	GCAATG	2 cut(s)	697, 2085
BseMII	CTCAG	5 cut(s)	15, 93, 662, 1349, 1500
BseNI	ACTGG	4 cut(s)	280, 903, 1799, 2596
BseRI	GAGGAG	3 cut(s)	41, 926, 1745
BseSI	GKGCMC	2 cut(s)	873, 1635
BseXI	GCAGC	9 cut(s)	340, 616, 674, 1387, 1396, 1756, 2103, 2191, 2585
BsgI	GTGCAG	1 cut(s)	732
Bsh1236I	CGCG	2 cut(s)	510, 564
BshFI	GGCC	5 cut(s)	14, 1188, 1633, 1862, 2140
BshVI	ATCGAT	1 cut(s)	1080
BsiHKAI	GWGCWC	1 cut(s)	1737
BsiSI	CCGG	2 cut(s)	158, 2196
BsiWI	CGTACG	1 cut(s)	362
BslFI	GGGAC	3 cut(s)	437, 1624, 2345
BslI	CCNNNNNNNGG	4 cut(s)	1184, 1558, 1982, 2536
BsmAI	GTCTC	4 cut(s)	66, 159, 1030, 1659
BsmFI	GGGAC	3 cut(s)	437, 1624, 2345
BsmI	GAATGC	1 cut(s)	1794
BsnI	GGCC	5 cut(s)	14, 1188, 1633, 1862, 2140
Bsp120I	GGGCCC	1 cut(s)	1631
Bsp1286I	GDGCHC	3 cut(s)	873, 1635, 1737
Bsp13I	TCCGGA	1 cut(s)	157
Bsp1407I	TGTACA	1 cut(s)	2127
Bsp1720I	GCTNAGC	1 cut(s)	5
Bsp19I	CCATGG	2 cut(s)	1597, 2009
Bsp68I	TCGCGA	1 cut(s)	564
BspACI	CCGC	3 cut(s)	46, 93, 1178
BspANI	GGCC	5 cut(s)	14, 1188, 1633, 1862, 2140
BspCNI	CTCAG	5 cut(s)	16, 94, 661, 1350, 1499
BspDI	ATCGAT	1 cut(s)	1080
BspEI	TCCGGA	1 cut(s)	157
BspFNI	CGCG	2 cut(s)	510, 564
BspHI	TCATGA	2 cut(s)	430, 1473
BspMAI	CTGCAG	1 cut(s)	2121
BspPI	GGATC	7 cut(s)	231, 666, 1460, 2325, 2410, 2417, 2423
BspQI	GCTCTTC	1 cut(s)	1430
BspTI	CTTAAG	1 cut(s)	1000
BsrDI	GCAATG	2 cut(s)	697, 2085
BsrGI	TGTACA	1 cut(s)	2127
BsrI	ACTGG	4 cut(s)	280, 903, 1799, 2596
BssECI	CCNNGG	7 cut(s)	1597, 1699, 2009, 2184, 2194, 2460, 2530
BssT1I	CCWWGG	5 cut(s)	1597, 1699, 2009, 2184, 2460
Bst2UI	CCWGG	2 cut(s)	2023, 2532
Bst4CI	ACNGT	7 cut(s)	193, 439, 797, 1374, 1615, 1696, 2443
Bst6I	CTCTTC	3 cut(s)	156, 547, 1430
BstAFI	CTTAAG	1 cut(s)	1000
BstAPI	GCANNNNNTGC	1 cut(s)	1789
BstAUI	TGTACA	1 cut(s)	2127
BstC8I	GCNNGC	7 cut(s)	1172, 1178, 1386, 1522, 1526, 2578, 2582
BstDEI	CTNAG	8 cut(s)	5, 24, 102, 648, 1163, 1358, 1486, 1566
BstDSI	CCRYGG	2 cut(s)	1597, 2009
BstEII	GGTNACC	1 cut(s)	2152
BstENI	CCTNNNNNAGG	2 cut(s)	1556, 1980
BstF5I	GGATG	7 cut(s)	214, 373, 423, 555, 607, 1840, 1947
BstFNI	CGCG	2 cut(s)	510, 564
BstH2I	RGCGCY	2 cut(s)	688, 1177
BstHHI	GCGC	2 cut(s)	687, 1176
BstMAI	GTCTC	4 cut(s)	66, 159, 1030, 1659
BstMWI	GCNNNNNNNGC	7 cut(s)	20, 250, 1406, 1409, 1415, 1789, 2273
BstNI	CCWGG	2 cut(s)	2023, 2532
BstNSI	RCATGY	2 cut(s)	1241, 2116
BstPI	GGTNACC	1 cut(s)	2152
BstSCI	CCNGG	3 cut(s)	2021, 2194, 2530
BstSFI	CTRYAG	5 cut(s)	192, 940, 1114, 1854, 2117
BstSLI	GKGCMC	2 cut(s)	873, 1635
BstUI	CGCG	2 cut(s)	510, 564
BstV1I	GCAGC	9 cut(s)	340, 616, 674, 1387, 1396, 1756, 2103, 2191, 2585
BstV2I	GAAGAC	2 cut(s)	196, 2241
BstX2I	RGATCY	6 cut(s)	196, 236, 658, 2317, 2415, 2422
BstXI	CCANNNNNNTGG	2 cut(s)	1604, 2164
BstYI	RGATCY	6 cut(s)	196, 236, 658, 2317, 2415, 2422
Bsu15I	ATCGAT	1 cut(s)	1080
BsuI	GTATCC	2 cut(s)	255, 1222
BsuRI	GGCC	5 cut(s)	14, 1188, 1633, 1862, 2140
BsuTUI	ATCGAT	1 cut(s)	1080
BtgI	CCRYGG	2 cut(s)	1597, 2009
BtgZI	GCGATG	1 cut(s)	579
BtsCI	GGATG	7 cut(s)	214, 373, 423, 555, 607, 1840, 1947
BtsI	GCAGTG	2 cut(s)	207, 1151
BtsIMutI	CAGTG	9 cut(s)	207, 910, 1068, 1151, 1355, 1370, 1692, 2405, 2589
BtuMI	TCGCGA	1 cut(s)	564
Cac8I	GCNNGC	7 cut(s)	1172, 1178, 1386, 1522, 1526, 2578, 2582
CaiI	CAGNNNCTG	1 cut(s)	1493
CciI	TCATGA	2 cut(s)	430, 1473
CfoI	GCGC	2 cut(s)	687, 1176
Cfr13I	GGNCC	8 cut(s)	31, 729, 1125, 1187, 1549, 1631, 1632, 2332
ClaI	ATCGAT	1 cut(s)	1080
CseI	GACGC	1 cut(s)	455
Csp6I	GTAC	6 cut(s)	363, 640, 719, 1483, 2128, 2524
CspCI	CAANNNNNGTGG	2 cut(s)	1624, 1659
CviQI	GTAC	6 cut(s)	363, 640, 719, 1483, 2128, 2524
DdeI	CTNAG	8 cut(s)	5, 24, 102, 648, 1163, 1358, 1486, 1566
DraIII	CACNNNGTG	1 cut(s)	2000
DriI	GACNNNNNGTC	1 cut(s)	2242
EaeI	YGGCCR	2 cut(s)	12, 2138
Eam1104I	CTCTTC	3 cut(s)	156, 547, 1430
Eam1105I	GACNNNNNGTC	1 cut(s)	2242
EarI	CTCTTC	3 cut(s)	156, 547, 1430
Ecl136II	GAGCTC	1 cut(s)	1735
Eco130I	CCWWGG	5 cut(s)	1597, 1699, 2009, 2184, 2460
Eco147I	AGGCCT	1 cut(s)	1862
Eco24I	GRGCYC	2 cut(s)	1635, 1737
Eco32I	GATATC	1 cut(s)	1245
Eco47I	GGWCC	5 cut(s)	31, 729, 1125, 1549, 2332
Eco53kI	GAGCTC	1 cut(s)	1735
Eco57I	CTGAAG	4 cut(s)	230, 320, 609, 2363
Eco91I	GGTNACC	1 cut(s)	2152
EcoICRI	GAGCTC	1 cut(s)	1735
EcoNI	CCTNNNNNAGG	2 cut(s)	1556, 1980
EcoO109I	RGGNCCY	3 cut(s)	729, 1549, 2332
EcoO65I	GGTNACC	1 cut(s)	2152
EcoRII	CCWGG	2 cut(s)	2021, 2530
EcoRV	GATATC	1 cut(s)	1245
EcoT14I	CCWWGG	5 cut(s)	1597, 1699, 2009, 2184, 2460
EcoT22I	ATGCAT	1 cut(s)	1794
EcoT38I	GRGCYC	2 cut(s)	1635, 1737
ErhI	CCWWGG	5 cut(s)	1597, 1699, 2009, 2184, 2460
FalI	AAGNNNNNCTT	2 cut(s)	59, 91
FaqI	GGGAC	3 cut(s)	437, 1624, 2345
FauI	CCCGC	1 cut(s)	1185
Fnu4HI	GCNGC	9 cut(s)	354, 630, 688, 1401, 1410, 1770, 2117, 2205, 2574
FokI	GGATG	7 cut(s)	201, 380, 430, 562, 614, 1847, 1954
FriOI	GRGCYC	2 cut(s)	1635, 1737
Fsp4HI	GCNGC	9 cut(s)	354, 630, 688, 1401, 1410, 1770, 2117, 2205, 2574
FspBI	CTAG	3 cut(s)	618, 1778, 1977
GlaI	GCGC	2 cut(s)	686, 1175
GluI	GCNGC	9 cut(s)	354, 630, 688, 1401, 1410, 1770, 2117, 2205, 2574
GsuI	CTGGAG	3 cut(s)	288, 1739, 1949
HaeII	RGCGCY	2 cut(s)	688, 1177
HaeIII	GGCC	5 cut(s)	14, 1188, 1633, 1862, 2140
HapII	CCGG	2 cut(s)	158, 2196
HgaI	GACGC	1 cut(s)	455
HhaI	GCGC	2 cut(s)	687, 1176
Hin6I	GCGC	2 cut(s)	685, 1174
HinP1I	GCGC	2 cut(s)	685, 1174
HincII	GTYRAC	1 cut(s)	475
HindII	GTYRAC	1 cut(s)	475
HindIII	AAGCTT	3 cut(s)	824, 1382, 2070
HpaII	CCGG	2 cut(s)	158, 2196
HphI	GGTGA	6 cut(s)	569, 821, 1715, 1797, 2146, 2368
Hpy166II	GTNNAC	2 cut(s)	475, 1826
Hpy8I	GTNNAC	2 cut(s)	475, 1826
Hpy99I	CGWCG	1 cut(s)	471
HpyAV	CCTTC	8 cut(s)	252, 620, 998, 1614, 2086, 2117, 2321, 2557
HpyCH4III	ACNGT	7 cut(s)	193, 439, 797, 1374, 1615, 1696, 2443
HpyCH4IV	ACGT	3 cut(s)	717, 844, 1333
HpyF10VI	GCNNNNNNNGC	7 cut(s)	20, 250, 1406, 1409, 1415, 1789, 2273
HpyF3I	CTNAG	8 cut(s)	5, 24, 102, 648, 1163, 1358, 1486, 1566
HpySE526I	ACGT	3 cut(s)	717, 844, 1333
HspAI	GCGC	2 cut(s)	685, 1174
KflI	GGGWCCC	1 cut(s)	2332
Kpn2I	TCCGGA	1 cut(s)	157
LguI	GCTCTTC	1 cut(s)	1430
LmnI	GCTCC	6 cut(s)	391, 989, 1271, 1303, 1408, 1732
Lsp1109I	GCAGC	9 cut(s)	340, 616, 674, 1387, 1396, 1756, 2103, 2191, 2585
LweI	GCATC	5 cut(s)	163, 733, 1072, 1105, 1492
MaeI	CTAG	3 cut(s)	618, 1778, 1977
MaeII	ACGT	3 cut(s)	717, 844, 1333
MaeIII	GTNAC	7 cut(s)	580, 592, 1690, 1992, 2152, 2309, 2437
MfeI	CAATTG	2 cut(s)	972, 1431
MflI	RGATCY	6 cut(s)	196, 236, 658, 2317, 2415, 2422
MhlI	GDGCHC	3 cut(s)	873, 1635, 1737
MlsI	TGGCCA	1 cut(s)	14
MluI	ACGCGT	1 cut(s)	508
MluNI	TGGCCA	1 cut(s)	14
MlyI	GAGTC	6 cut(s)	113, 385, 1371, 2000, 2346, 2483
MmeI	TCCRAC	4 cut(s)	413, 1597, 1919, 2380
Mox20I	TGGCCA	1 cut(s)	14
Mph1103I	ATGCAT	1 cut(s)	1794
MroI	TCCGGA	1 cut(s)	157
MroXI	GAANNNNTTC	1 cut(s)	510
MscI	TGGCCA	1 cut(s)	14
MseI	TTAA	5 cut(s)	147, 275, 501, 1001, 2596
MslI	CAYNNNNRTG	3 cut(s)	1071, 1286, 1602
Msp20I	TGGCCA	1 cut(s)	14
MspA1I	CMGCKG	4 cut(s)	1520, 1769, 2489, 2576
MspCI	CTTAAG	1 cut(s)	1000
MspI	CCGG	2 cut(s)	158, 2196
MspR9I	CCNGG	3 cut(s)	2023, 2196, 2532
MunI	CAATTG	2 cut(s)	972, 1431
Mva1269I	GAATGC	1 cut(s)	1794
MvaI	CCWGG	2 cut(s)	2023, 2532
MvnI	CGCG	2 cut(s)	510, 564
MwoI	GCNNNNNNNGC	7 cut(s)	20, 250, 1406, 1409, 1415, 1789, 2273
NciI	CCSGG	1 cut(s)	2196
NcoI	CCATGG	2 cut(s)	1597, 2009
NmeAIII	GCCGAG	1 cut(s)	1399
NmuCI	GTSAC	6 cut(s)	592, 1690, 1992, 2152, 2309, 2437
NruI	TCGCGA	1 cut(s)	564
NsiI	ATGCAT	1 cut(s)	1794
NspI	RCATGY	2 cut(s)	1241, 2116
PagI	TCATGA	2 cut(s)	430, 1473
PceI	AGGCCT	1 cut(s)	1862
PciI	ACATGT	1 cut(s)	1237
PciSI	GCTCTTC	1 cut(s)	1430
PctI	GAATGC	1 cut(s)	1794
PdmI	GAANNNNTTC	1 cut(s)	510
PfeI	GAWTC	5 cut(s)	346, 802, 1055, 1490, 2366
Pfl23II	CGTACG	1 cut(s)	362
PkrI	GCNGC	9 cut(s)	355, 631, 689, 1402, 1411, 1771, 2118, 2206, 2575
PleI	GAGTC	6 cut(s)	112, 385, 1370, 1999, 2346, 2482
PpsI	GAGTC	6 cut(s)	112, 385, 1370, 1999, 2346, 2482
PpuMI	RGGWCCY	3 cut(s)	729, 1549, 2332
PscI	ACATGT	1 cut(s)	1237
PshBI	ATTAAT	1 cut(s)	501
PsiI	TTATAA	1 cut(s)	1556
Psp124BI	GAGCTC	1 cut(s)	1737
Psp1406I	AACGTT	1 cut(s)	844
Psp5II	RGGWCCY	3 cut(s)	729, 1549, 2332
Psp6I	CCWGG	2 cut(s)	2021, 2530
PspEI	GGTNACC	1 cut(s)	2152
PspGI	CCWGG	2 cut(s)	2021, 2530
PspLI	CGTACG	1 cut(s)	362
PspOMI	GGGCCC	1 cut(s)	1631
PspPI	GGNCC	8 cut(s)	31, 729, 1125, 1187, 1549, 1631, 1632, 2332
PspPPI	RGGWCCY	3 cut(s)	729, 1549, 2332
PstI	CTGCAG	1 cut(s)	2121
PstNI	CAGNNNCTG	1 cut(s)	1493
PsuI	RGATCY	6 cut(s)	196, 236, 658, 2317, 2415, 2422
PvuII	CAGCTG	4 cut(s)	1520, 1769, 2489, 2576
RruI	TCGCGA	1 cut(s)	564
RsaI	GTAC	6 cut(s)	364, 641, 720, 1484, 2129, 2525
RsaNI	GTAC	6 cut(s)	363, 640, 719, 1483, 2128, 2524
RseI	CAYNNNNRTG	3 cut(s)	1071, 1286, 1602
SacI	GAGCTC	1 cut(s)	1737
SapI	GCTCTTC	1 cut(s)	1430
SaqAI	TTAA	5 cut(s)	147, 275, 501, 1001, 2596
SatI	GCNGC	9 cut(s)	354, 630, 688, 1401, 1410, 1770, 2117, 2205, 2574
Sau96I	GGNCC	8 cut(s)	31, 729, 1125, 1187, 1549, 1631, 1632, 2332
SchI	GAGTC	6 cut(s)	113, 385, 1371, 2000, 2346, 2483
ScrFI	CCNGG	3 cut(s)	2023, 2196, 2532
SduI	GDGCHC	3 cut(s)	873, 1635, 1737
SfaNI	GCATC	5 cut(s)	163, 733, 1072, 1105, 1492
SfcI	CTRYAG	5 cut(s)	192, 940, 1114, 1854, 2117
SinI	GGWCC	5 cut(s)	31, 729, 1125, 1549, 2332
SmiMI	CAYNNNNRTG	3 cut(s)	1071, 1286, 1602
SmlI	CTYRAG	3 cut(s)	125, 733, 1000
SmoI	CTYRAG	3 cut(s)	125, 733, 1000
SseBI	AGGCCT	1 cut(s)	1862
SsiI	CCGC	3 cut(s)	46, 93, 1178
SspMI	CTAG	3 cut(s)	618, 1778, 1977
SstI	GAGCTC	1 cut(s)	1737
StuI	AGGCCT	1 cut(s)	1862
StyD4I	CCNGG	3 cut(s)	2021, 2194, 2530
StyI	CCWWGG	5 cut(s)	1597, 1699, 2009, 2184, 2460
TaaI	ACNGT	7 cut(s)	193, 439, 797, 1374, 1615, 1696, 2443
TaiI	ACGT	3 cut(s)	720, 847, 1336
TaqI	TCGA	2 cut(s)	930, 1080
TatI	WGTACW	2 cut(s)	639, 2127
TfiI	GAWTC	5 cut(s)	346, 802, 1055, 1490, 2366
Tru1I	TTAA	5 cut(s)	147, 275, 501, 1001, 2596
Tru9I	TTAA	5 cut(s)	147, 275, 501, 1001, 2596
TscAI	CASTG	9 cut(s)	214, 910, 1075, 1151, 1362, 1377, 1699, 2412, 2596
TseFI	GTSAC	6 cut(s)	592, 1690, 1992, 2152, 2309, 2437
TseI	GCWGC	9 cut(s)	353, 629, 687, 1400, 1409, 1769, 2116, 2204, 2573
Tsp45I	GTSAC	6 cut(s)	592, 1690, 1992, 2152, 2309, 2437
TspGWI	ACGGA	7 cut(s)	221, 280, 380, 692, 1676, 1898, 2486
TspRI	CASTG	9 cut(s)	214, 910, 1075, 1151, 1362, 1377, 1699, 2412, 2596
Vha464I	CTTAAG	1 cut(s)	1000
VpaK11BI	GGWCC	5 cut(s)	31, 729, 1125, 1549, 2332
VspI	ATTAAT	1 cut(s)	501
XagI	CCTNNNNNAGG	2 cut(s)	1556, 1980
XapI	RAATTY	3 cut(s)	88, 143, 761
XceI	RCATGY	2 cut(s)	1241, 2116
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1604, 1815
XmnI	GAANNNNTTC	1 cut(s)	510
XspI	CTAG	3 cut(s)	618, 1778, 1977
Zsp2I	ATGCAT	1 cut(s)	1794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.