Rorug05G0122600

Phosphoenolpyruvate carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004914
Physical Location & Seq
Reverse (-)
10847017 .. 10847292
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0122600.1

Sequence Viewer

Length: 276 bp
ATGAGGCCTCTTCTACCACAAGTTGATCCAGTTGTGTACAACATGCTTCAAGAAGATCCAGGCAATGTGAGCTTCTCGCCTGTCGGAGACCTATCTGATCAGATTCAAGATCTAAGGGAATCTATGGAGCTACCTTTGATGAACCCTGAGCTGTTTCATAGGGTGGGGATTAAGCCTCCCAAGGGCGTTCTTCTCTATGGACCTCCGGGAACAGGAAAGACATTACTAGCTAGAGCAATTTCGTATCAAAGTGCCATTGTTTGTATGTTGGGGTGA

Protein Analysis

91

Amino Acids

9.96

Weight (kDa)

5.12

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 63 - 83 3.1e-06 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 20, 50
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 182, 212
AgsI TTSAA 2 cut(s) 50, 107
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 4 cut(s) 72, 130, 151, 230
AluI AGCT 4 cut(s) 72, 130, 151, 230
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 2 cut(s) 20, 50
AoxI GGCC 1 cut(s) 5
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 60
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 81
BfaI CTAG 2 cut(s) 227, 231
BglII AGATCT 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 60, 207
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 60, 207
Bpu10I CCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 180
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 212
Bse1I ACTGG 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 182, 212
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 1 cut(s) 29
BshFI GGCC 1 cut(s) 7
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 182, 212
BsmAI GTCTC 1 cut(s) 81
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 81
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 4 cut(s) 25, 55, 97, 109
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 1 cut(s) 139
BspPI GGATC 2 cut(s) 20, 50
BspTNI GGTCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 4 cut(s) 25, 55, 97, 109
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 60
Bst6I CTCTTC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 36
BstDEI CTNAG 2 cut(s) 113, 147
BstKTI GATC 4 cut(s) 28, 58, 100, 112
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 4 cut(s) 25, 55, 97, 109
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 58, 205
BstX2I RGATCY 2 cut(s) 55, 109
BstYI RGATCY 2 cut(s) 55, 109
BsuRI GGCC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 37
CviAII CATG 1 cut(s) 43
CviJI RGCY 6 cut(s) 7, 72, 130, 151, 175, 230
CviKI_1 RGCY 6 cut(s) 7, 72, 130, 151, 175, 230
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 2 cut(s) 113, 147
DpnI GATC 4 cut(s) 27, 57, 99, 111
DpnII GATC 4 cut(s) 25, 55, 97, 109
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 7
Eco31I GGTCTC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 125, 159, 198, 266
FatI CATG 1 cut(s) 42
FbaI TGATCA 1 cut(s) 97
FspBI CTAG 2 cut(s) 227, 231
HaeIII GGCC 1 cut(s) 7
HapII CCGG 1 cut(s) 206
Hin1II CATG 1 cut(s) 46
HinfI GANTC 2 cut(s) 103, 119
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 86, 97, 102
Hpy188III TCNNGA 2 cut(s) 50, 107
Hpy8I GTNNAC 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 2 cut(s) 113, 147
Hsp92II CATG 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 4 cut(s) 25, 55, 97, 109
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 7 cut(s) 42, 45, 72, 93, 159, 198, 219
MaeI CTAG 2 cut(s) 227, 231
MalI GATC 4 cut(s) 27, 57, 99, 111
MboI GATC 4 cut(s) 25, 55, 97, 109
MboII GAAGA 2 cut(s) 65, 182
MflI RGATCY 2 cut(s) 55, 109
MluCI AATT 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 3 cut(s) 18, 186, 213
MseI TTAA 1 cut(s) 171
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 60, 207
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 69
NciI CCSGG 1 cut(s) 207
NdeII GATC 4 cut(s) 25, 55, 97, 109
NlaIII CATG 1 cut(s) 46
NspI RCATGY 1 cut(s) 46
PceI AGGCCT 1 cut(s) 7
PfeI GAWTC 2 cut(s) 103, 119
PfoI TCCNGGA 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspPI GGNCC 1 cut(s) 200
PsuI RGATCY 2 cut(s) 55, 109
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 171
Sau3AI GATC 4 cut(s) 25, 55, 97, 109
Sau96I GGNCC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 60, 207
SetI ASST 7 cut(s) 74, 93, 132, 136, 153, 205, 232
SinI GGWCC 1 cut(s) 200
Sse9I AATT 1 cut(s) 237
SseBI AGGCCT 1 cut(s) 7
SspMI CTAG 2 cut(s) 227, 231
StuI AGGCCT 1 cut(s) 7
StyD4I CCNGG 2 cut(s) 58, 205
StyI CCWWGG 1 cut(s) 180
TasI AATT 1 cut(s) 237
TatI WGTACW 1 cut(s) 36
TfiI GAWTC 2 cut(s) 103, 119
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TspDTI ATGAA 2 cut(s) 146, 155
VpaK11BI GGWCC 1 cut(s) 200
XceI RCATGY 1 cut(s) 46
XspI CTAG 2 cut(s) 227, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.