AT1G54310

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008663
Physical Location & Seq
Forward (+)
20273567 .. 20275574
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54310.2

Sequence Viewer

Length: 738 bp
ATGGTGATGCTCGGTGTGCTCCAGCTTCCCTGCCGCCTCCATCACTCTCTCTTTTACTCCTCTTCCCAAATTGCTTCTTCTCGCCCTCAAGGTGTTGCTAAAGTGGTTCTGAAAAAAGGCAAGACACAGCTCTTCAAAGACGGAAGTCCAATGGTGTACAGTGGAGCTGTCGACAGGATCATCGGAAAACCACCTCCTCAGACAGGAGATGTTGTCATTGTAGCTGATGGCACTGAGAATCCCATTGGTTGGGGTTTGTATAACTCTGTTTCTATGTTCTGTGTTCGCCTTATGCAGCTTCAACACGAATCCACCAGAGATCCTTCTTGTGCATTGAACATTGAGAAGCTTCTCCAAACAAGAATCGCTGAAGCAGTTCAGTTGCGAAAGAGTTTGGCACTTCCCTCGGCTAATACTAATGCTTATCGTCTTGTCAACAGTGAAGGAGATAGATTGTCTGGATTGATTGTGGATGTGTTTGGAGATATAGCTGTGGTAGCATCCTCTGCTGCTTGGCTTGAAAAGTACAGGATTGAAGTGGAGAGTTGCTTAAGATCAATTGACGGAATTAATCATATAAACTGGAGACCTTCTCTTGATGTTCTTAAAGAAGATGGTTTTGACATCTCAAGTCTGAAACAGACACAATCATCTACTCTCCCACAGAGATCAATGGTACGGTGTGTGGTGCCCTTAATCAGGTTGTGGAAAATGGGATCTCATACGCAATTTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

245

Amino Acids

27.11

Weight (kDa)

9.28

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 52 - 115 3.4e-11 PUA-like domain
Methyltrans_SAM PF10672 243 - 401 1.3e-17 S-adenosylmethionine-dependent methyltransferase
MTS PF05175 273 - 371 8.4e-06 Methyltransferase small domain
PrmA PF06325 275 - 355 3.4e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 276 - 404 3.4e-09 Methyltransferase domain
Cons_hypoth95 PF03602 277 - 359 1e-07 Conserved hypothetical protein 95
Methyltransf_25 PF13649 281 - 356 5.3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 249
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 34
AclWI GGATC 3 cut(s) 185, 314, 724
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 3 cut(s) 158, 527, 678
AfiI CCNNNNNNNGG 3 cut(s) 203, 249, 699
AflII CTTAAG 1 cut(s) 550
AgsI TTSAA 5 cut(s) 136, 302, 337, 521, 536
AluBI AGCT 7 cut(s) 25, 130, 167, 224, 298, 349, 491
AluI AGCT 7 cut(s) 25, 130, 167, 224, 298, 349, 491
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 580
AlwI GGATC 3 cut(s) 185, 314, 724
ApeKI GCWGC 2 cut(s) 295, 509
AseI ATTAAT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 693
BanI GGYRCC 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 307, 496
BccI CCATC 3 cut(s) 48, 221, 608
BcgI CGANNNNNNTGC 2 cut(s) 387, 421
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 1 cut(s) 736
BfrI CTTAAG 1 cut(s) 550
BisI GCNGC 3 cut(s) 34, 296, 510
BlsI GCNGC 3 cut(s) 35, 297, 511
BmiI GGNNCC 1 cut(s) 690
BmsI GCATC 1 cut(s) 509
BoxI GACNNNNGTC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpmI CTGGAG 2 cut(s) 5, 604
BpuEI CTTGAG 2 cut(s) 72, 613
BsaI GGTCTC 1 cut(s) 580
BsaJI CCNNGG 1 cut(s) 405
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 249, 699
Bse1I ACTGG 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 2 cut(s) 478, 500
BseLI CCNNNNNNNGG 3 cut(s) 203, 249, 699
BseMII CTCAG 2 cut(s) 212, 225
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 2 cut(s) 49, 186
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 2 cut(s) 307, 496
BshNI GGYRCC 1 cut(s) 688
BsiHKAI GWGCWC 1 cut(s) 21
BslI CCNNNNNNNGG 3 cut(s) 203, 249, 699
BsmAI GTCTC 1 cut(s) 580
Bso31I GGTCTC 1 cut(s) 580
Bsp1286I GDGCHC 2 cut(s) 21, 693
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 5 cut(s) 177, 319, 554, 668, 716
BspACI CCGC 1 cut(s) 34
BspCNI CTCAG 2 cut(s) 211, 226
BspLI GGNNCC 1 cut(s) 690
BspPI GGATC 3 cut(s) 185, 314, 724
BspQI GCTCTTC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 688
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 587
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 5 cut(s) 177, 319, 554, 668, 716
Bst4CI ACNGT 3 cut(s) 161, 440, 681
Bst6I CTCTTC 2 cut(s) 67, 137
BstAFI CTTAAG 1 cut(s) 550
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 156
BstDEI CTNAG 2 cut(s) 198, 234
BstENI CCTNNNNNAGG 2 cut(s) 201, 697
BstF5I GGATG 2 cut(s) 478, 500
BstKTI GATC 5 cut(s) 180, 322, 557, 671, 719
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 5 cut(s) 177, 319, 554, 668, 716
BstMWI GCNNNNNNNGC 3 cut(s) 16, 497, 506
BstPAI GACNNNNGTC 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 693
BstV1I GCAGC 2 cut(s) 307, 496
BstX2I RGATCY 2 cut(s) 319, 716
BstYI RGATCY 2 cut(s) 319, 716
BtsCI GGATG 2 cut(s) 478, 500
BtsIMutI CAGTG 3 cut(s) 166, 231, 445
Csp6I GTAC 3 cut(s) 157, 526, 677
CviJI RGCY 9 cut(s) 25, 130, 167, 224, 298, 349, 410, 491, 517
CviKI_1 RGCY 9 cut(s) 25, 130, 167, 224, 298, 349, 410, 491, 517
CviQI GTAC 3 cut(s) 157, 526, 677
DdeI CTNAG 2 cut(s) 198, 234
DpnI GATC 5 cut(s) 179, 321, 556, 670, 718
DpnII GATC 5 cut(s) 177, 319, 554, 668, 716
Eam1104I CTCTTC 2 cut(s) 67, 137
EarI CTCTTC 2 cut(s) 67, 137
Eco31I GGTCTC 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 390
EcoNI CCTNNNNNAGG 2 cut(s) 201, 697
FaiI YATR 7 cut(s) 261, 275, 293, 488, 576, 578, 723
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 34, 296, 510
FokI GGATG 2 cut(s) 485, 487
Fsp4HI GCNGC 3 cut(s) 34, 296, 510
FspBI CTAG 1 cut(s) 736
GluI GCNGC 3 cut(s) 34, 296, 510
GsuI CTGGAG 2 cut(s) 5, 604
HincII GTYRAC 2 cut(s) 172, 436
HindII GTYRAC 2 cut(s) 172, 436
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 238, 308, 363
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 157, 172, 436
Hpy188I TCNGA 4 cut(s) 111, 185, 201, 636
Hpy188III TCNNGA 2 cut(s) 459, 596
Hpy8I GTNNAC 3 cut(s) 157, 172, 436
HpyAV CCTTC 3 cut(s) 333, 437, 600
HpyCH4III ACNGT 3 cut(s) 161, 440, 681
HpyCH4V TGCA 2 cut(s) 295, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 497, 506
HpyF3I CTNAG 2 cut(s) 198, 234
Kzo9I GATC 5 cut(s) 177, 319, 554, 668, 716
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 24, 164
LpnPI CCDG 9 cut(s) 35, 43, 160, 189, 328, 444, 514, 568, 685
Lsp1109I GCAGC 2 cut(s) 307, 496
LweI GCATC 1 cut(s) 509
MaeI CTAG 1 cut(s) 736
MalI GATC 5 cut(s) 179, 321, 556, 670, 718
MboI GATC 5 cut(s) 177, 319, 554, 668, 716
MboII GAAGA 4 cut(s) 54, 69, 124, 623
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 2 cut(s) 319, 716
MhlI GDGCHC 2 cut(s) 21, 693
MluCI AATT 4 cut(s) 69, 558, 567, 728
MnlI CCTC 7 cut(s) 47, 70, 96, 204, 207, 415, 514
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 4 cut(s) 551, 570, 606, 695
MspCI CTTAAG 1 cut(s) 550
MunI CAATTG 1 cut(s) 558
MwoI GCNNNNNNNGC 3 cut(s) 16, 497, 506
NdeII GATC 5 cut(s) 177, 319, 554, 668, 716
NlaIV GGNNCC 1 cut(s) 690
NmeAIII GCCGAG 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 238, 308, 363
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 3 cut(s) 35, 297, 511
PshAI GACNNNNGTC 1 cut(s) 144
PshBI ATTAAT 1 cut(s) 570
PspN4I GGNNCC 1 cut(s) 690
PsuI RGATCY 2 cut(s) 319, 716
RsaI GTAC 3 cut(s) 158, 527, 678
RsaNI GTAC 3 cut(s) 157, 526, 677
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 4 cut(s) 551, 570, 606, 695
SatI GCNGC 3 cut(s) 34, 296, 510
Sau3AI GATC 5 cut(s) 177, 319, 554, 668, 716
SduI GDGCHC 2 cut(s) 21, 693
SfaNI GCATC 1 cut(s) 509
SmlI CTYRAG 3 cut(s) 87, 550, 628
SmoI CTYRAG 3 cut(s) 87, 550, 628
Sse9I AATT 4 cut(s) 69, 558, 567, 728
SsiI CCGC 1 cut(s) 34
SspMI CTAG 1 cut(s) 736
TaaI ACNGT 3 cut(s) 161, 440, 681
TaqI TCGA 1 cut(s) 171
TasI AATT 4 cut(s) 69, 558, 567, 728
TatI WGTACW 2 cut(s) 156, 525
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 3 cut(s) 238, 308, 363
Tru1I TTAA 4 cut(s) 551, 570, 606, 695
Tru9I TTAA 4 cut(s) 551, 570, 606, 695
TscAI CASTG 3 cut(s) 166, 238, 445
TseI GCWGC 2 cut(s) 295, 509
TspGWI ACGGA 2 cut(s) 156, 579
TspRI CASTG 3 cut(s) 166, 238, 445
Van91I CCANNNNNTGG 1 cut(s) 249
Vha464I CTTAAG 1 cut(s) 550
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 2 cut(s) 201, 697
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.