RLG00000032720

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008663
Physical Location & Seq
Reverse (-)
18191827 .. 18196137
4311 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032720

Sequence Viewer

Length: 1326 bp
ATGCAGCATTTTCCAGCCCGCCTGATAAAGTCTCTGTCTACTTCTACTTCCTTACCATGCACCACCACTACCCTTCAAGAACTTGCTTCTGCTCACCCCAAAGGTGTTGCGAGGGTTGTACTCAAGAAGGGGAAGACTCAACTATTCAAGGATGGAAGTCCAATGGTTTATAGTGGAGCAGTTGATAGAATAATTGGGAGACCGCCACCAAGAACTGGAGATGTTGTGCTAGTAGCTGATGGAGCAGAAAAGCCAATAGGGTGGGGCATGTATAATTCAGTCTCCATGTTCTGTGTCCGGCTTATGCAGCTGGATGAGGAAGCAATAAGGGATTGTTCATGTGCATTGAATATGGAGAAACTGCTTGAGACAAGAATTAATGAAGCTATAGAGCTACGTAAGAGTTTGGGGCTTCCATCAGCTAGTACAAATGCATTTCGTCTTGTCAATAGTGAAGGAGACAGATTATCAGGCCTAATAATTGACATATTTGGAGATGTAGCAGTAATAGCATCATCTGCCGCTTGGGTTGAGAAGTACAAAGCAGAAATAGAGGCTTGTGTTGGTAGAATCAATGAAATTAATCACATCAACTGGAGACCGTCTGTGGAAATTTTAAAAGAAGAAGGATTGGATGTGTCAAATTTGAAGGAAATGGATCCATCTATTTGCCCTCAGAGAACAAAGGTTATGGAAAACGGGATTTTTTATGCCATTTCACTTGAAGGGCAGAAGACAGGGTTCTATGCTGATCAGCGTGAAAACCGCAAATTCATATCAACAATTTCAGACAATCAGAAAGTTCTTGATATCTGCTGCTATAGTGGTGGTTTTGCGCTAAATGCTGCACGAGGTGGTGCTGTGAATGTCACAGGTGTTGACTCATCCTTGGGTGCAATCGAATTAGCTAAAGAAAATATTGCTCTTAACAACATGGATCCAGGAATAATATCATTTGTGAAAGAAGATGCAAGTGAGTTTATGAAGGGTGCTCTTTCTAGGAATGAATCATTTGACATTGTCATAATAGATCCTCCTAAACTAGCACCACGAAAGAAGGTTTTACAAAATGCATCAGGCATGTATAGAAATTTAAATTCATTAGCAATGCAATTGACAAGGAAAGGTGGCCTTCTCATGACTTGTTCGTGTTCAGGAGCGATGACCCAAAGTGGGACGTTCTTGCGCATTCTTCAGAGTGCAGCATCAGCAGCAGGTAGAAAAATAACCGTTCTCAGGGATGCTGGAGCAGCCTGTGATCATACCATTGACCCATCCTACCCAGAAGGCAAATACCTTTCCAACATTCTCCTGAGGGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

442

Amino Acids

47.88

Weight (kDa)

8.21

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 39 - 102 4.1e-10 PUA-like domain
Methyltrans_SAM PF10672 229 - 389 3.3e-16 S-adenosylmethionine-dependent methyltransferase
TRM5-TYW2_MTfase PF02475 230 - 347 2.5e-06 TRM5/TYW2 methyltransferase domain
MTS PF05175 260 - 361 2.6e-08 Methyltransferase small domain
PrmA PF06325 262 - 342 5e-08 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 263 - 392 2.4e-11 Methyltransferase domain
Cons_hypoth95 PF03602 264 - 346 1.1e-08 Conserved hypothetical protein 95
Methyltransf_25 PF13649 268 - 354 1.2e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1187
Acc36I ACCTGC 1 cut(s) 1205
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 38
AciI CCGC 4 cut(s) 19, 203, 522, 766
AclWI GGATC 5 cut(s) 653, 666, 932, 945, 1025
AcsI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
AcuI CTGAAG 1 cut(s) 1178
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 3 cut(s) 120, 427, 539
AfiI CCNNNNNNNGG 3 cut(s) 215, 1173, 1236
AgsI TTSAA 5 cut(s) 77, 148, 349, 649, 725
AjnI CCWGG 1 cut(s) 940
AluBI AGCT 6 cut(s) 236, 310, 386, 394, 422, 908
AluI AGCT 6 cut(s) 236, 310, 386, 394, 422, 908
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
AlwI GGATC 5 cut(s) 653, 666, 932, 945, 1025
AoxI GGCC 2 cut(s) 472, 1129
ApeKI GCWGC 7 cut(s) 4, 307, 816, 845, 1202, 1211, 1250
ApoI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
AseI ATTAAT 2 cut(s) 378, 582
AspLEI GCGC 2 cut(s) 838, 1188
AsuHPI GGTGA 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 1313
BamHI GGATCC 2 cut(s) 658, 937
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 140, 740
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BccI CCATC 5 cut(s) 146, 233, 424, 670, 1282
BciT130I CCWGG 1 cut(s) 942
BclI TGATCA 2 cut(s) 751, 1258
BcoDI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BfaI CTAG 4 cut(s) 230, 423, 999, 1043
BfmI CTRYAG 2 cut(s) 387, 820
BfuAI ACCTGC 1 cut(s) 1205
BisI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
BlsI GCNGC 8 cut(s) 6, 309, 523, 818, 847, 1204, 1213, 1252
Bme1390I CCNGG 1 cut(s) 942
BmiI GGNNCC 2 cut(s) 660, 939
BmrFI CCNGG 1 cut(s) 942
BmsI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
BpiI GAAGAC 2 cut(s) 140, 740
BpmI CTGGAG 3 cut(s) 237, 616, 1266
BpuEI CTTGAG 2 cut(s) 107, 386
BsaAI YACGTR 1 cut(s) 398
BsaI GGTCTC 2 cut(s) 193, 592
BsaJI CCNNGG 1 cut(s) 888
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 1173, 1236
Bse1I ACTGG 2 cut(s) 220, 599
Bse21I CCTNAGG 1 cut(s) 1313
Bse3DI GCAATG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 942
BseDI CCNNGG 1 cut(s) 888
BseGI GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BseLI CCNNNNNNNGG 3 cut(s) 215, 1173, 1236
BseMI GCAATG 1 cut(s) 1113
BseMII CTCAG 3 cut(s) 689, 1249, 1304
BseNI ACTGG 2 cut(s) 220, 599
BseXI GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BsgI GTGCAG 2 cut(s) 831, 1221
BshFI GGCC 2 cut(s) 474, 1131
BsiHKAI GWGCWC 1 cut(s) 994
BsiSI CCGG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 1189
BslI CCNNNNNNNGG 3 cut(s) 215, 1173, 1236
BsmAI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BsmFI GGGAC 1 cut(s) 1189
BsmI GAATGC 1 cut(s) 1188
BsnI GGCC 2 cut(s) 474, 1131
Bso31I GGTCTC 2 cut(s) 193, 592
Bsp1286I GDGCHC 1 cut(s) 994
Bsp143I GATC 5 cut(s) 658, 751, 937, 1030, 1258
BspACI CCGC 4 cut(s) 19, 203, 522, 766
BspANI GGCC 2 cut(s) 474, 1131
BspCNI CTCAG 3 cut(s) 688, 1248, 1305
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 2 cut(s) 660, 939
BspMI ACCTGC 1 cut(s) 1205
BspPI GGATC 5 cut(s) 653, 666, 932, 945, 1025
BspTNI GGTCTC 2 cut(s) 193, 592
BsrDI GCAATG 1 cut(s) 1113
BsrI ACTGG 2 cut(s) 220, 599
BssECI CCNNGG 1 cut(s) 888
BssMI GATC 5 cut(s) 658, 751, 937, 1030, 1258
BssSI CACGAG 1 cut(s) 849
BssT1I CCWWGG 1 cut(s) 888
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 2 cut(s) 603, 1231
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 3 cut(s) 675, 1235, 1313
BstF5I GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BstHHI GCGC 2 cut(s) 838, 1188
BstKTI GATC 5 cut(s) 661, 754, 940, 1033, 1261
BstMAI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BstMBI GATC 5 cut(s) 658, 751, 937, 1030, 1258
BstMWI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
BstNI CCWGG 1 cut(s) 942
BstNSI RCATGY 2 cut(s) 271, 1084
BstSCI CCNGG 1 cut(s) 940
BstSFI CTRYAG 2 cut(s) 387, 820
BstSNI TACGTA 1 cut(s) 398
BstV1I GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BstV2I GAAGAC 2 cut(s) 140, 740
BstX2I RGATCY 3 cut(s) 658, 937, 1030
BstXI CCANNNNNNTGG 1 cut(s) 261
BstYI RGATCY 3 cut(s) 658, 937, 1030
Bsu36I CCTNAGG 1 cut(s) 1313
BsuRI GGCC 2 cut(s) 474, 1131
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BveI ACCTGC 1 cut(s) 1205
Cac8I GCNNGC 1 cut(s) 19
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 2 cut(s) 838, 1188
Csp6I GTAC 3 cut(s) 119, 426, 538
CviAII CATG 7 cut(s) 57, 268, 286, 339, 934, 1081, 1138
CviQI GTAC 3 cut(s) 119, 426, 538
DdeI CTNAG 3 cut(s) 675, 1235, 1313
DpnI GATC 5 cut(s) 660, 753, 939, 1032, 1260
DpnII GATC 5 cut(s) 658, 751, 937, 1030, 1258
DraI TTTAAA 2 cut(s) 618, 1095
DraIII CACNNNGTG 1 cut(s) 854
Eco105I TACGTA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 888
Eco147I AGGCCT 1 cut(s) 474
Eco31I GGTCTC 2 cut(s) 193, 592
Eco32I GATATC 1 cut(s) 811
Eco57I CTGAAG 1 cut(s) 1178
Eco81I CCTNAGG 1 cut(s) 1313
EcoRII CCWGG 1 cut(s) 940
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 1 cut(s) 888
EcoT22I ATGCAT 2 cut(s) 436, 1075
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 7 cut(s) 60, 271, 289, 342, 937, 1084, 1141
FalI AAGNNNNNCTT 2 cut(s) 1116, 1148
FaqI GGGAC 1 cut(s) 1189
FatI CATG 7 cut(s) 56, 267, 285, 338, 933, 1080, 1137
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 2 cut(s) 751, 1258
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
FokI GGATG 6 cut(s) 164, 326, 647, 871, 1253, 1261
Fsp4HI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
FspBI CTAG 4 cut(s) 230, 423, 999, 1043
FspI TGCGCA 1 cut(s) 1187
GlaI GCGC 2 cut(s) 837, 1187
GluI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
GsuI CTGGAG 3 cut(s) 237, 616, 1266
HaeIII GGCC 2 cut(s) 474, 1131
HapII CCGG 1 cut(s) 298
HhaI GCGC 2 cut(s) 838, 1188
Hin1II CATG 7 cut(s) 60, 271, 289, 342, 937, 1084, 1141
Hin6I GCGC 2 cut(s) 836, 1186
HinP1I GCGC 2 cut(s) 836, 1186
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 4 cut(s) 136, 570, 881, 1007
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 2 cut(s) 39, 880
Hpy188I TCNGA 4 cut(s) 678, 790, 798, 1197
Hpy188III TCNNGA 6 cut(s) 77, 124, 806, 1138, 1155, 1312
Hpy8I GTNNAC 2 cut(s) 39, 880
HpyCH4III ACNGT 2 cut(s) 603, 1231
HpyCH4IV ACGT 2 cut(s) 397, 1178
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
HpyF3I CTNAG 3 cut(s) 675, 1235, 1313
HpySE526I ACGT 2 cut(s) 397, 1178
Hsp92II CATG 7 cut(s) 60, 271, 289, 342, 937, 1084, 1141
HspAI GCGC 2 cut(s) 836, 1186
Ksp22I TGATCA 2 cut(s) 751, 1258
Kzo9I GATC 5 cut(s) 658, 751, 937, 1030, 1258
LmnI GCTCC 4 cut(s) 176, 242, 1157, 1247
Lsp1109I GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
LweI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
MaeI CTAG 4 cut(s) 230, 423, 999, 1043
MaeII ACGT 2 cut(s) 397, 1178
MaeIII GTNAC 1 cut(s) 868
MalI GATC 5 cut(s) 660, 753, 939, 1032, 1260
MboI GATC 5 cut(s) 658, 751, 937, 1030, 1258
MboII GAAGA 5 cut(s) 145, 635, 745, 977, 1184
MfeI CAATTG 1 cut(s) 1112
MflI RGATCY 3 cut(s) 658, 937, 1030
MhlI GDGCHC 1 cut(s) 994
MlyI GAGTC 2 cut(s) 130, 875
MmeI TCCRAC 1 cut(s) 1326
MnlI CCTC 7 cut(s) 105, 310, 547, 684, 845, 1044, 1308
Mph1103I ATGCAT 2 cut(s) 436, 1075
MseI TTAA 5 cut(s) 378, 582, 617, 927, 1094
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 298
MspR9I CCNGG 1 cut(s) 942
MunI CAATTG 1 cut(s) 1112
Mva1269I GAATGC 1 cut(s) 1188
MvaI CCWGG 1 cut(s) 942
MwoI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
NdeII GATC 5 cut(s) 658, 751, 937, 1030, 1258
NlaIII CATG 7 cut(s) 60, 271, 289, 342, 937, 1084, 1141
NlaIV GGNNCC 2 cut(s) 660, 939
NmuCI GTSAC 1 cut(s) 868
NsbI TGCGCA 1 cut(s) 1187
NsiI ATGCAT 2 cut(s) 436, 1075
NspI RCATGY 2 cut(s) 271, 1084
PagI TCATGA 1 cut(s) 1137
PceI AGGCCT 1 cut(s) 474
PctI GAATGC 1 cut(s) 1188
PfeI GAWTC 2 cut(s) 570, 1007
PflFI GACNNNGTC 1 cut(s) 1019
PflMI CCANNNNNTGG 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 8 cut(s) 6, 309, 523, 818, 847, 1204, 1213, 1252
PleI GAGTC 2 cut(s) 130, 875
PpsI GAGTC 2 cut(s) 130, 875
Ppu21I YACGTR 1 cut(s) 398
PshBI ATTAAT 2 cut(s) 378, 582
Psp6I CCWGG 1 cut(s) 940
PspGI CCWGG 1 cut(s) 940
PspN4I GGNNCC 2 cut(s) 660, 939
PsuI RGATCY 3 cut(s) 658, 937, 1030
PsyI GACNNNGTC 1 cut(s) 1019
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 3 cut(s) 120, 427, 539
RsaNI GTAC 3 cut(s) 119, 426, 538
SaqAI TTAA 5 cut(s) 378, 582, 617, 927, 1094
SatI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
Sau3AI GATC 5 cut(s) 658, 751, 937, 1030, 1258
SchI GAGTC 2 cut(s) 130, 875
ScrFI CCNGG 1 cut(s) 942
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
SfcI CTRYAG 2 cut(s) 387, 820
SmiI ATTTAAAT 1 cut(s) 1095
SmlI CTYRAG 2 cut(s) 122, 365
SmoI CTYRAG 2 cut(s) 122, 365
SnaBI TACGTA 1 cut(s) 398
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 4 cut(s) 19, 203, 522, 766
SspI AATATT 1 cut(s) 919
SspMI CTAG 4 cut(s) 230, 423, 999, 1043
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 940
StyI CCWWGG 1 cut(s) 888
SwaI ATTTAAAT 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 603, 1231
TaiI ACGT 2 cut(s) 400, 1181
TaqI TCGA 1 cut(s) 900
TatI WGTACW 3 cut(s) 118, 425, 537
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 570, 1007
Tru1I TTAA 5 cut(s) 378, 582, 617, 927, 1094
Tru9I TTAA 5 cut(s) 378, 582, 617, 927, 1094
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 7 cut(s) 4, 307, 816, 845, 1202, 1211, 1250
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 7 cut(s) 327, 396, 591, 763, 998, 1020, 1089
Tth111I GACNNNGTC 1 cut(s) 1019
Van91I CCANNNNNTGG 1 cut(s) 215
VspI ATTAAT 2 cut(s) 378, 582
XapI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
XceI RCATGY 2 cut(s) 271, 1084
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 4 cut(s) 230, 423, 999, 1043
Zsp2I ATGCAT 2 cut(s) 436, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.