Rw5G015090

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008663
Physical Location & Seq
Reverse (-)
18242854 .. 18247055
4202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015090.1

Sequence Viewer

Length: 1326 bp
ATGCAGCATTTTCCAGCCCGCCTGATAAAGTCTCTGTCTACTTCTACTTCCTTACCATGCACCACCACTACCCTTCAAGAACTTGCTTCTGCTCACCCCAAAGGTGTTGCGAGGGTTGTACTCAAGAAGGGGAAGACACAACTATTCAAGGATGGAAGTCCAATGGTTTATAGTGGAGCAGTTGATAGAATAATTGGGAGACCACCACCAAGAACTGGAGATGTTGTGCTAGTAGCTGATGGAGCAGAAAAGCCAATAGGGTGGGGCATGTATAATTCAGTCTCCATGTTCTGTGTCCGGCTTATGCAGCTGGATGAGGAAGCAATAAGGGATTGTTCATGTGCATTGAATATGGAGAAACTGCTTGAGACAAGAATTAATGAAGCTATAGAGCTACGTAAGAGTTTGGGGCTTCCATCAGCTAGTACAAATGCATTTCGTCTTGTCAATAGTGAAGGAGACAGATTATCAGGCCTAATAATTGACATATTTGGAGATGTAGCAGTAATAGCATCATCTGCCGCTTGGGTTGAGAAGTACAAAGCAGAAATAGAGGCTTGTGTTGGTAGAATCAATGAAATTAATCACATCAACTGGAGACCGTCTGTGGAAATTTTAAAAGAAGAAGGATTGGATGTGTCAAATTTGAAGGAAATGGATCCATCGATTTGCCCTCAGAGAACAAAGGTTATGGAAAACGGGATTTTTTATGCCATTTCACTTGAAGGGCAGAAGACAGGGTTCTATGCTGATCAGCGTGAAAACCGCAAATTCATATCAACAATTTCAGACAATCAGAAAGTTCTTGATATCTGCTGCTATAGTGGTGGTTTTGCGCTAAATGCTGCACGAGGTGGTGCTGTGAATGTCACAGGTGTTGACTCATCCTTCGGTGCAATCGAACTAGCTAAAGAAAATATTGCTCTTAACAACATGGATCCAGGAATAATATCATTTGTGAAAGAAGATGCAAGTGAGTTTATGAAGGGTGCTCTTTCTAGGAATGAATCATTTGATATTGTCATCTTAGATCCTCCTAAACTAGCACCACGAAAGAAGGTTTTACAAAATGCATCAGGCATGTATAGAAATTTAAATTCATTAGCAATGCAATTGACAAGGAGAGGTGGCCTTCTCATGACTTGTTCATGTTCAGGAGCGATGACCCAAAGTGGGACGTTCTTGCGCATTCTTCAGAGTGCAGCATCAGCAGCAGGTAGAAAAATAACTGTTCTCAGGGATGCTGGAGCAGCATGTGATCATACCATTGACCCATCCTACCCAGAAGGCAAATACCTTTCCAACATTCTCCTGAGGGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

441

Amino Acids

47.94

Weight (kDa)

8.21

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 39 - 102 4.1e-10 PUA-like domain
Methyltrans_SAM PF10672 229 - 389 7.5e-16 S-adenosylmethionine-dependent methyltransferase
TRM5-TYW2_MTfase PF02475 230 - 347 2e-06 TRM5/TYW2 methyltransferase domain
MTS PF05175 260 - 358 4e-08 Methyltransferase small domain
PrmA PF06325 262 - 342 5.9e-08 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 263 - 391 1.1e-10 Methyltransferase domain
Cons_hypoth95 PF03602 264 - 346 1.2e-08 Conserved hypothetical protein 95
Methyltransf_25 PF13649 268 - 353 1.7e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1187
Acc36I ACCTGC 1 cut(s) 1205
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 38
AciI CCGC 3 cut(s) 19, 522, 766
AclWI GGATC 5 cut(s) 653, 666, 932, 945, 1025
AcsI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
AcuI CTGAAG 1 cut(s) 1178
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 3 cut(s) 120, 427, 539
AfiI CCNNNNNNNGG 2 cut(s) 215, 1173
AgsI TTSAA 5 cut(s) 77, 148, 349, 649, 725
AjnI CCWGG 1 cut(s) 940
AluBI AGCT 6 cut(s) 236, 310, 386, 394, 422, 908
AluI AGCT 6 cut(s) 236, 310, 386, 394, 422, 908
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
AlwI GGATC 5 cut(s) 653, 666, 932, 945, 1025
AoxI GGCC 2 cut(s) 472, 1129
ApeKI GCWGC 7 cut(s) 4, 307, 816, 845, 1202, 1211, 1250
ApoI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
AseI ATTAAT 2 cut(s) 378, 582
AspLEI GCGC 2 cut(s) 838, 1188
AsuHPI GGTGA 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 1313
BamHI GGATCC 2 cut(s) 658, 937
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 140, 740
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BccI CCATC 5 cut(s) 146, 233, 424, 670, 1282
BciT130I CCWGG 1 cut(s) 942
BclI TGATCA 2 cut(s) 751, 1258
BcoDI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BfaI CTAG 5 cut(s) 230, 423, 905, 999, 1043
BfmI CTRYAG 2 cut(s) 387, 820
BfuAI ACCTGC 1 cut(s) 1205
BisI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
BlsI GCNGC 8 cut(s) 6, 309, 523, 818, 847, 1204, 1213, 1252
Bme1390I CCNGG 1 cut(s) 942
BmiI GGNNCC 2 cut(s) 660, 939
BmrFI CCNGG 1 cut(s) 942
BmsI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
BpiI GAAGAC 2 cut(s) 140, 740
BpmI CTGGAG 3 cut(s) 237, 616, 1266
BpuEI CTTGAG 2 cut(s) 107, 386
Bsa29I ATCGAT 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 398
BsaI GGTCTC 2 cut(s) 193, 592
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 1173
Bse1I ACTGG 2 cut(s) 220, 599
Bse21I CCTNAGG 1 cut(s) 1313
Bse3DI GCAATG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 942
BseCI ATCGAT 1 cut(s) 665
BseGI GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BseLI CCNNNNNNNGG 2 cut(s) 215, 1173
BseMI GCAATG 1 cut(s) 1113
BseMII CTCAG 3 cut(s) 689, 1249, 1304
BseNI ACTGG 2 cut(s) 220, 599
BseXI GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BsgI GTGCAG 2 cut(s) 831, 1221
BshFI GGCC 2 cut(s) 474, 1131
BshVI ATCGAT 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 994
BsiSI CCGG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 1189
BslI CCNNNNNNNGG 2 cut(s) 215, 1173
BsmAI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BsmFI GGGAC 1 cut(s) 1189
BsmI GAATGC 1 cut(s) 1188
BsnI GGCC 2 cut(s) 474, 1131
Bso31I GGTCTC 2 cut(s) 193, 592
Bsp1286I GDGCHC 1 cut(s) 994
Bsp143I GATC 5 cut(s) 658, 751, 937, 1030, 1258
BspACI CCGC 3 cut(s) 19, 522, 766
BspANI GGCC 2 cut(s) 474, 1131
BspCNI CTCAG 3 cut(s) 688, 1248, 1305
BspDI ATCGAT 1 cut(s) 665
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 2 cut(s) 660, 939
BspMI ACCTGC 1 cut(s) 1205
BspPI GGATC 5 cut(s) 653, 666, 932, 945, 1025
BspTNI GGTCTC 2 cut(s) 193, 592
BsrDI GCAATG 1 cut(s) 1113
BsrI ACTGG 2 cut(s) 220, 599
BssMI GATC 5 cut(s) 658, 751, 937, 1030, 1258
BssSI CACGAG 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 2 cut(s) 603, 1231
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 675, 1027, 1235, 1313
BstF5I GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BstHHI GCGC 2 cut(s) 838, 1188
BstKTI GATC 5 cut(s) 661, 754, 940, 1033, 1261
BstMAI GTCTC 6 cut(s) 36, 193, 286, 362, 453, 592
BstMBI GATC 5 cut(s) 658, 751, 937, 1030, 1258
BstMWI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
BstNI CCWGG 1 cut(s) 942
BstNSI RCATGY 3 cut(s) 271, 1084, 1257
BstSCI CCNGG 1 cut(s) 940
BstSFI CTRYAG 2 cut(s) 387, 820
BstSNI TACGTA 1 cut(s) 398
BstV1I GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
BstV2I GAAGAC 2 cut(s) 140, 740
BstX2I RGATCY 3 cut(s) 658, 937, 1030
BstXI CCANNNNNNTGG 1 cut(s) 261
BstYI RGATCY 3 cut(s) 658, 937, 1030
Bsu15I ATCGAT 1 cut(s) 665
Bsu36I CCTNAGG 1 cut(s) 1313
BsuRI GGCC 2 cut(s) 474, 1131
BsuTUI ATCGAT 1 cut(s) 665
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 6 cut(s) 157, 319, 640, 884, 1246, 1274
BveI ACCTGC 1 cut(s) 1205
Cac8I GCNNGC 1 cut(s) 19
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 2 cut(s) 838, 1188
ClaI ATCGAT 1 cut(s) 665
Csp6I GTAC 3 cut(s) 119, 426, 538
CviAII CATG 9 cut(s) 57, 268, 286, 339, 934, 1081, 1138, 1149, 1254
CviQI GTAC 3 cut(s) 119, 426, 538
DdeI CTNAG 4 cut(s) 675, 1027, 1235, 1313
DpnI GATC 5 cut(s) 660, 753, 939, 1032, 1260
DpnII GATC 5 cut(s) 658, 751, 937, 1030, 1258
DraI TTTAAA 2 cut(s) 618, 1095
DraIII CACNNNGTG 1 cut(s) 854
Eco105I TACGTA 1 cut(s) 398
Eco147I AGGCCT 1 cut(s) 474
Eco31I GGTCTC 2 cut(s) 193, 592
Eco32I GATATC 1 cut(s) 811
Eco57I CTGAAG 1 cut(s) 1178
Eco81I CCTNAGG 1 cut(s) 1313
EcoRII CCWGG 1 cut(s) 940
EcoRV GATATC 1 cut(s) 811
EcoT22I ATGCAT 2 cut(s) 436, 1075
FaeI CATG 9 cut(s) 60, 271, 289, 342, 937, 1084, 1141, 1152, 1257
FaqI GGGAC 1 cut(s) 1189
FatI CATG 9 cut(s) 56, 267, 285, 338, 933, 1080, 1137, 1148, 1253
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 2 cut(s) 751, 1258
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
FokI GGATG 6 cut(s) 164, 326, 647, 871, 1253, 1261
Fsp4HI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
FspBI CTAG 5 cut(s) 230, 423, 905, 999, 1043
FspI TGCGCA 1 cut(s) 1187
GlaI GCGC 2 cut(s) 837, 1187
GluI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
GsuI CTGGAG 3 cut(s) 237, 616, 1266
HaeIII GGCC 2 cut(s) 474, 1131
HapII CCGG 1 cut(s) 298
HhaI GCGC 2 cut(s) 838, 1188
Hin1II CATG 9 cut(s) 60, 271, 289, 342, 937, 1084, 1141, 1152, 1257
Hin6I GCGC 2 cut(s) 836, 1186
HinP1I GCGC 2 cut(s) 836, 1186
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 3 cut(s) 570, 881, 1007
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 2 cut(s) 39, 880
Hpy188I TCNGA 4 cut(s) 678, 790, 798, 1197
Hpy188III TCNNGA 6 cut(s) 77, 124, 806, 1138, 1155, 1312
Hpy8I GTNNAC 2 cut(s) 39, 880
HpyCH4III ACNGT 2 cut(s) 603, 1231
HpyCH4IV ACGT 2 cut(s) 397, 1178
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
HpyF3I CTNAG 4 cut(s) 675, 1027, 1235, 1313
HpySE526I ACGT 2 cut(s) 397, 1178
Hsp92II CATG 9 cut(s) 60, 271, 289, 342, 937, 1084, 1141, 1152, 1257
HspAI GCGC 2 cut(s) 836, 1186
Ksp22I TGATCA 2 cut(s) 751, 1258
Kzo9I GATC 5 cut(s) 658, 751, 937, 1030, 1258
LmnI GCTCC 4 cut(s) 176, 242, 1157, 1247
Lsp1109I GCAGC 7 cut(s) 16, 319, 803, 832, 1214, 1223, 1262
LweI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
MaeI CTAG 5 cut(s) 230, 423, 905, 999, 1043
MaeII ACGT 2 cut(s) 397, 1178
MaeIII GTNAC 1 cut(s) 868
MalI GATC 5 cut(s) 660, 753, 939, 1032, 1260
MboI GATC 5 cut(s) 658, 751, 937, 1030, 1258
MboII GAAGA 5 cut(s) 145, 635, 745, 977, 1184
MfeI CAATTG 1 cut(s) 1112
MflI RGATCY 3 cut(s) 658, 937, 1030
MhlI GDGCHC 1 cut(s) 994
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 1 cut(s) 1326
MnlI CCTC 8 cut(s) 105, 310, 547, 684, 845, 1044, 1118, 1308
Mph1103I ATGCAT 2 cut(s) 436, 1075
MseI TTAA 5 cut(s) 378, 582, 617, 927, 1094
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 298
MspR9I CCNGG 1 cut(s) 942
MunI CAATTG 1 cut(s) 1112
Mva1269I GAATGC 1 cut(s) 1188
MvaI CCWGG 1 cut(s) 942
MwoI GCNNNNNNNGC 8 cut(s) 242, 307, 509, 518, 842, 1208, 1211, 1250
NdeII GATC 5 cut(s) 658, 751, 937, 1030, 1258
NlaIII CATG 9 cut(s) 60, 271, 289, 342, 937, 1084, 1141, 1152, 1257
NlaIV GGNNCC 2 cut(s) 660, 939
NmuCI GTSAC 1 cut(s) 868
NsbI TGCGCA 1 cut(s) 1187
NsiI ATGCAT 2 cut(s) 436, 1075
NspI RCATGY 3 cut(s) 271, 1084, 1257
PagI TCATGA 1 cut(s) 1137
PceI AGGCCT 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 897
PctI GAATGC 1 cut(s) 1188
PfeI GAWTC 2 cut(s) 570, 1007
PflMI CCANNNNNTGG 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 8 cut(s) 6, 309, 523, 818, 847, 1204, 1213, 1252
PleI GAGTC 1 cut(s) 875
PpsI GAGTC 1 cut(s) 875
Ppu21I YACGTR 1 cut(s) 398
PshBI ATTAAT 2 cut(s) 378, 582
Psp6I CCWGG 1 cut(s) 940
PspGI CCWGG 1 cut(s) 940
PspN4I GGNNCC 2 cut(s) 660, 939
PsuI RGATCY 3 cut(s) 658, 937, 1030
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 3 cut(s) 120, 427, 539
RsaNI GTAC 3 cut(s) 119, 426, 538
SaqAI TTAA 5 cut(s) 378, 582, 617, 927, 1094
SatI GCNGC 8 cut(s) 5, 308, 522, 817, 846, 1203, 1212, 1251
Sau3AI GATC 5 cut(s) 658, 751, 937, 1030, 1258
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 1 cut(s) 942
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 5 cut(s) 521, 958, 1082, 1214, 1231
SfcI CTRYAG 2 cut(s) 387, 820
SmiI ATTTAAAT 1 cut(s) 1095
SmlI CTYRAG 2 cut(s) 122, 365
SmoI CTYRAG 2 cut(s) 122, 365
SnaBI TACGTA 1 cut(s) 398
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 3 cut(s) 19, 522, 766
SspI AATATT 1 cut(s) 919
SspMI CTAG 5 cut(s) 230, 423, 905, 999, 1043
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 940
SwaI ATTTAAAT 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 603, 1231
TaiI ACGT 2 cut(s) 400, 1181
TaqI TCGA 2 cut(s) 665, 900
TatI WGTACW 3 cut(s) 118, 425, 537
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 570, 1007
Tru1I TTAA 5 cut(s) 378, 582, 617, 927, 1094
Tru9I TTAA 5 cut(s) 378, 582, 617, 927, 1094
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 7 cut(s) 4, 307, 816, 845, 1202, 1211, 1250
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 8 cut(s) 327, 396, 591, 763, 998, 1020, 1089, 1137
Van91I CCANNNNNTGG 1 cut(s) 215
VspI ATTAAT 2 cut(s) 378, 582
XapI RAATTY 5 cut(s) 612, 643, 770, 1090, 1096
XceI RCATGY 3 cut(s) 271, 1084, 1257
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 5 cut(s) 230, 423, 905, 999, 1043
Zsp2I ATGCAT 2 cut(s) 436, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.