Rroxscaffold_1G00055930

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008663
Physical Location & Seq
Forward (+)
77616072 .. 77620593
4522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00055930.1

Sequence Viewer

Length: 1098 bp
ATGCAGCATTTTCCAGCCCGCCTGATAAAGTCCCTGTCTACTTCTGCTTCCTTACCATGCACCACCACTACCCTCCAAGAACTTGCTTCTGCTCACCCCAAAGGTGTTGCGAGGGTTGTACTCAAGAAGGGGAAGACACAGCTATTCAAGGATGGAAGTCCAATGGTTTATAGTGGAGCAGTTGATAGAATAATTGGGAGACCGCCACCAAGAACTGGAGATGTTGTGCTAGTAGCTGATGGAGCAGAAAAGCCAATAGGGTGGGGCATGTATAATTCAGTCTCCATGTTCTGTGTCCGGCTTATGCAGCTGGATGAGGAAGCAATAAGGGATTGTTCATGTGCATTGAATATGGAGAAACTGCTTGAGACAAGAATTAATGAAGCTATAGAACTACGTAAGAGTTTGGGGCTTCCATCAGCTAGTACAAATGCATTTCGTCTTGTCAATAGCCTAATAATTGACATATTTGGAGATGTAGCAGTAATAGCATCATCTGCCGCTTGGGTTGAGAAGTACAAAGCAGAAATAGAGGCTTTTGTTGGTAGAATCAATGAAATTAATCACATCAACTGGAGACCGTCTGTGGAAATTTTAAAAGAAGAAGGATTGGATGTGTCAAATTTGAAGGAAATGGATCCATCTATTTGCCCTCAGAGAACAAAGGTTATGGAAAACGGGATTTTTTATGCCATTTCACTTGAAGGGCAGAAGACAGGGTTCTATGCTGATCAGCGTGAAAACCGCAAATTCATATCAACAATTTCAGACAATCAGAAAGTTCTTGATATCTGCTGCTATAGTGGTGGTTTTGCGCTAAATGCTGCACGAGGTGGTGCTGTGAATGTCACAGGATTAATATCATTTGTGAAAGAAGATGCAAGTGAGTTTATGAAGGGTGCTCTTTCTAGGAATGAATCATTTGATATTGTCATCTTAGATCCTCCTAAACTAGCACCACGAAAGAAGAGTGCAGCATCAGCAGCAGGTAGAAAAATAACCGTTCTCAGGGATGCTGGAGCAGCATGTGATCATACCATTGACCCATCCTACCCAGAAGGCAAATACCTTTCCAACATTCTCCTGAGGGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrans_SAM

Protein Analysis

365

Amino Acids

39.87

Weight (kDa)

8.33

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUA_3 PF17785 39 - 102 3.1e-10 PUA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 977
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 38
AciI CCGC 4 cut(s) 19, 203, 501, 745
AclWI GGATC 3 cut(s) 632, 645, 935
AcsI RAATTY 3 cut(s) 591, 622, 749
AdeI CACNNNGTG 1 cut(s) 833
AfaI GTAC 3 cut(s) 120, 427, 518
AfiI CCNNNNNNNGG 2 cut(s) 215, 1008
AgsI TTSAA 4 cut(s) 148, 349, 628, 704
AluBI AGCT 5 cut(s) 142, 236, 310, 386, 422
AluI AGCT 5 cut(s) 142, 236, 310, 386, 422
Alw21I GWGCWC 1 cut(s) 904
Alw26I GTCTC 4 cut(s) 193, 286, 362, 571
AlwI GGATC 3 cut(s) 632, 645, 935
ApeKI GCWGC 7 cut(s) 4, 307, 795, 824, 974, 983, 1022
ApoI RAATTY 3 cut(s) 591, 622, 749
AseI ATTAAT 3 cut(s) 378, 561, 857
AspLEI GCGC 1 cut(s) 817
AsuHPI GGTGA 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 1085
BamHI GGATCC 1 cut(s) 637
BauI CACGAG 1 cut(s) 828
BbsI GAAGAC 2 cut(s) 140, 719
Bbv12I GWGCWC 1 cut(s) 904
BbvI GCAGC 7 cut(s) 16, 319, 782, 811, 986, 995, 1034
BccI CCATC 5 cut(s) 146, 233, 424, 649, 1054
BclI TGATCA 2 cut(s) 730, 1030
BcoDI GTCTC 4 cut(s) 193, 286, 362, 571
BfaI CTAG 4 cut(s) 230, 423, 909, 953
BfmI CTRYAG 2 cut(s) 387, 799
BfuAI ACCTGC 1 cut(s) 977
BisI GCNGC 8 cut(s) 5, 308, 501, 796, 825, 975, 984, 1023
BlsI GCNGC 8 cut(s) 6, 309, 502, 797, 826, 976, 985, 1024
BmiI GGNNCC 1 cut(s) 639
BmsI GCATC 4 cut(s) 500, 868, 986, 1003
BpiI GAAGAC 2 cut(s) 140, 719
BpmI CTGGAG 3 cut(s) 237, 595, 1038
BpuEI CTTGAG 2 cut(s) 107, 386
BsaAI YACGTR 1 cut(s) 398
BsaBI GATNNNNATC 1 cut(s) 859
BsaI GGTCTC 2 cut(s) 193, 571
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 1008
Bse1I ACTGG 2 cut(s) 220, 578
Bse21I CCTNAGG 1 cut(s) 1085
Bse8I GATNNNNATC 1 cut(s) 859
BseGI GGATG 5 cut(s) 157, 319, 619, 1018, 1046
BseJI GATNNNNATC 1 cut(s) 859
BseLI CCNNNNNNNGG 2 cut(s) 215, 1008
BseMII CTCAG 3 cut(s) 668, 1021, 1076
BseNI ACTGG 2 cut(s) 220, 578
BseXI GCAGC 7 cut(s) 16, 319, 782, 811, 986, 995, 1034
BsgI GTGCAG 2 cut(s) 810, 993
BsiHKAI GWGCWC 1 cut(s) 904
BsiSI CCGG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 2 cut(s) 215, 1008
BsmAI GTCTC 4 cut(s) 193, 286, 362, 571
BsmFI GGGAC 1 cut(s) 16
Bso31I GGTCTC 2 cut(s) 193, 571
Bsp1286I GDGCHC 1 cut(s) 904
Bsp143I GATC 4 cut(s) 637, 730, 940, 1030
BspACI CCGC 4 cut(s) 19, 203, 501, 745
BspCNI CTCAG 3 cut(s) 667, 1020, 1077
BspLI GGNNCC 1 cut(s) 639
BspMI ACCTGC 1 cut(s) 977
BspPI GGATC 3 cut(s) 632, 645, 935
BspTNI GGTCTC 2 cut(s) 193, 571
BsrI ACTGG 2 cut(s) 220, 578
BssMI GATC 4 cut(s) 637, 730, 940, 1030
BssSI CACGAG 1 cut(s) 828
Bst2BI CACGAG 1 cut(s) 828
Bst4CI ACNGT 2 cut(s) 582, 1003
Bst6I CTCTTC 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 497
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 654, 937, 1007, 1085
BstF5I GGATG 5 cut(s) 157, 319, 619, 1018, 1046
BstHHI GCGC 1 cut(s) 817
BstKTI GATC 4 cut(s) 640, 733, 943, 1033
BstMAI GTCTC 4 cut(s) 193, 286, 362, 571
BstMBI GATC 4 cut(s) 637, 730, 940, 1030
BstMWI GCNNNNNNNGC 8 cut(s) 242, 307, 488, 497, 821, 980, 983, 1022
BstNSI RCATGY 2 cut(s) 271, 1029
BstSFI CTRYAG 2 cut(s) 387, 799
BstSNI TACGTA 1 cut(s) 398
BstV1I GCAGC 7 cut(s) 16, 319, 782, 811, 986, 995, 1034
BstV2I GAAGAC 2 cut(s) 140, 719
BstX2I RGATCY 2 cut(s) 637, 940
BstXI CCANNNNNNTGG 1 cut(s) 261
BstYI RGATCY 2 cut(s) 637, 940
Bsu36I CCTNAGG 1 cut(s) 1085
BtsCI GGATG 5 cut(s) 157, 319, 619, 1018, 1046
BveI ACCTGC 1 cut(s) 977
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 1 cut(s) 817
Csp6I GTAC 3 cut(s) 119, 426, 517
CviAII CATG 5 cut(s) 57, 268, 286, 339, 1026
CviQI GTAC 3 cut(s) 119, 426, 517
DdeI CTNAG 4 cut(s) 654, 937, 1007, 1085
DpnI GATC 4 cut(s) 639, 732, 942, 1032
DpnII GATC 4 cut(s) 637, 730, 940, 1030
DraI TTTAAA 1 cut(s) 597
DraIII CACNNNGTG 1 cut(s) 833
Eam1104I CTCTTC 1 cut(s) 962
EarI CTCTTC 1 cut(s) 962
Eco105I TACGTA 1 cut(s) 398
Eco31I GGTCTC 2 cut(s) 193, 571
Eco32I GATATC 1 cut(s) 790
Eco81I CCTNAGG 1 cut(s) 1085
EcoRV GATATC 1 cut(s) 790
EcoT22I ATGCAT 1 cut(s) 436
FaeI CATG 5 cut(s) 60, 271, 289, 342, 1029
FaqI GGGAC 1 cut(s) 16
FatI CATG 5 cut(s) 56, 267, 285, 338, 1025
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 2 cut(s) 730, 1030
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 8 cut(s) 5, 308, 501, 796, 825, 975, 984, 1023
FokI GGATG 5 cut(s) 164, 326, 626, 1025, 1033
Fsp4HI GCNGC 8 cut(s) 5, 308, 501, 796, 825, 975, 984, 1023
FspBI CTAG 4 cut(s) 230, 423, 909, 953
GlaI GCGC 1 cut(s) 816
GluI GCNGC 8 cut(s) 5, 308, 501, 796, 825, 975, 984, 1023
GsuI CTGGAG 3 cut(s) 237, 595, 1038
HapII CCGG 1 cut(s) 298
HhaI GCGC 1 cut(s) 817
Hin1II CATG 5 cut(s) 60, 271, 289, 342, 1029
Hin6I GCGC 1 cut(s) 815
HinP1I GCGC 1 cut(s) 815
HinfI GANTC 2 cut(s) 549, 917
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 3 cut(s) 657, 769, 777
Hpy188III TCNNGA 3 cut(s) 124, 785, 1084
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 6 cut(s) 121, 599, 622, 698, 889, 1052
HpyCH4III ACNGT 2 cut(s) 582, 1003
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 8 cut(s) 4, 60, 307, 344, 434, 827, 881, 974
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 307, 488, 497, 821, 980, 983, 1022
HpyF3I CTNAG 4 cut(s) 654, 937, 1007, 1085
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 5 cut(s) 60, 271, 289, 342, 1029
HspAI GCGC 1 cut(s) 815
Ksp22I TGATCA 2 cut(s) 730, 1030
Kzo9I GATC 4 cut(s) 637, 730, 940, 1030
LmnI GCTCC 3 cut(s) 176, 242, 1019
Lsp1109I GCAGC 7 cut(s) 16, 319, 782, 811, 986, 995, 1034
LweI GCATC 4 cut(s) 500, 868, 986, 1003
MaeI CTAG 4 cut(s) 230, 423, 909, 953
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 1 cut(s) 847
MalI GATC 4 cut(s) 639, 732, 942, 1032
MboI GATC 4 cut(s) 637, 730, 940, 1030
MboII GAAGA 5 cut(s) 145, 614, 724, 887, 979
MflI RGATCY 2 cut(s) 637, 940
MhlI GDGCHC 1 cut(s) 904
MluCI AATT 9 cut(s) 192, 274, 375, 459, 558, 591, 622, 749, 762
MmeI TCCRAC 1 cut(s) 1098
MnlI CCTC 8 cut(s) 83, 105, 310, 526, 663, 824, 954, 1080
Mph1103I ATGCAT 1 cut(s) 436
MseI TTAA 4 cut(s) 378, 561, 596, 857
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 1 cut(s) 298
MwoI GCNNNNNNNGC 8 cut(s) 242, 307, 488, 497, 821, 980, 983, 1022
NdeII GATC 4 cut(s) 637, 730, 940, 1030
NlaIII CATG 5 cut(s) 60, 271, 289, 342, 1029
NlaIV GGNNCC 1 cut(s) 639
NmuCI GTSAC 1 cut(s) 847
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 2 cut(s) 271, 1029
PfeI GAWTC 2 cut(s) 549, 917
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 8 cut(s) 6, 309, 502, 797, 826, 976, 985, 1024
Ppu21I YACGTR 1 cut(s) 398
PshBI ATTAAT 3 cut(s) 378, 561, 857
PspN4I GGNNCC 1 cut(s) 639
PsuI RGATCY 2 cut(s) 637, 940
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 3 cut(s) 120, 427, 518
RsaNI GTAC 3 cut(s) 119, 426, 517
SaqAI TTAA 4 cut(s) 378, 561, 596, 857
SatI GCNGC 8 cut(s) 5, 308, 501, 796, 825, 975, 984, 1023
Sau3AI GATC 4 cut(s) 637, 730, 940, 1030
SduI GDGCHC 1 cut(s) 904
SfaNI GCATC 4 cut(s) 500, 868, 986, 1003
SfcI CTRYAG 2 cut(s) 387, 799
SmlI CTYRAG 2 cut(s) 122, 365
SmoI CTYRAG 2 cut(s) 122, 365
SnaBI TACGTA 1 cut(s) 398
Sse9I AATT 9 cut(s) 192, 274, 375, 459, 558, 591, 622, 749, 762
SsiI CCGC 4 cut(s) 19, 203, 501, 745
SspMI CTAG 4 cut(s) 230, 423, 909, 953
TaaI ACNGT 2 cut(s) 582, 1003
TaiI ACGT 1 cut(s) 400
TasI AATT 9 cut(s) 192, 274, 375, 459, 558, 591, 622, 749, 762
TatI WGTACW 3 cut(s) 118, 425, 516
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 2 cut(s) 549, 917
Tru1I TTAA 4 cut(s) 378, 561, 596, 857
Tru9I TTAA 4 cut(s) 378, 561, 596, 857
TseFI GTSAC 1 cut(s) 847
TseI GCWGC 7 cut(s) 4, 307, 795, 824, 974, 983, 1022
Tsp45I GTSAC 1 cut(s) 847
TspDTI ATGAA 6 cut(s) 327, 396, 570, 742, 908, 930
Van91I CCANNNNNTGG 1 cut(s) 215
VspI ATTAAT 3 cut(s) 378, 561, 857
XapI RAATTY 3 cut(s) 591, 622, 749
XceI RCATGY 2 cut(s) 271, 1029
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 4 cut(s) 230, 423, 909, 953
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.