AT1G56590

adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009630
Physical Location & Seq
Reverse (-)
21201948 .. 21204856
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56590.1

Sequence Viewer

Length: 1248 bp
ATGCTTCAATGTATCTTTCTCATCTCCGATTCTGGAGAGGTAATGCTAGAGAAGCAGCTTACTGGTCATCGCGTTGATAGATCCATATGTGCTTGGTTCTGGGATCAATATATTTCTCAAGGCGATTCCTTTAAGGCACTTCCAGTGATTGCTTCACCAACACATTATCTATTTCAAATTGTTCGTGACGGAATTACGTTCTTAGCTTGTAGTCAAGTTGAAATGCCACCGTTGATGGCAATCGAGTTCCTTTGCAGAGTTGCTGATGTTTTGTCTGAGTACCTTGGTGGGCTAAATGAAGATCTCATTAAGGATAATTTCATCATTGTCTATGAGCTTTTGGATGAGATGATCGATAATGGTTTCCCTCTCACAACAGAACCTAGTATCCTGAAGGAAATGATAGCTCCACCGAATCTAGTCAGCAAAATGTTGAGTGTTGTAACGGGAAATGCTTCGAATGTTAGTGACACGCTTCCGAGTGGGGCGGGCTCTTGTGTTCCTTGGCGACCGACAGATCCAAAGTACTCTAGCAACGAAGTTTATGTCGACCTTGTTGAAGAGATGGATGCAATTGTAAACAGGGATGGAGAATTGGTAAAATGCGAGATTTATGGCGAAGTCCAAATGAATTCTCAGCTCACTGGTTTTCCAGATTTGACATTGTCGTTTGCGAATCCATCAATCCTAGAAGACATGAGGTTTCACCCGTGTGTCCGCTACAGACCATGGGAATCTCATCAAGTTCTCTCCTTTGTCCCTCCAGATGGAGAGTTCAAGCTTATGAGTTACAGGGTGAAGAAGCTAAAGAACACACCTGTATATGTAAAACCTCAAATAACATCAGATTCGGGTACATGTCGAATTAGCGTGCTAGTGGGAATCCGAAGCGATCCAGGGAAAACAATTGAGTCGATAACCTTGAGTTTCCAGCTTCCTCACTGTGTATCATCTGCAGATCTCTCATCAAATCATGGAACTGTAACAATCCTCTCTAACAAGACATGTACATGGACAATCGGGCGAATCCCAAAGGACAAAACTCCGTGTTTGTCGGGAACACTAGCGCTGGAACCAGGGTTAGAACGGCTTCACGTGTTTCCGACATTCAAACTCGGGTTTAAGATAATGGGCATTGCTCTTTCTGGCCTAAGAATCGAGAAACTTGATCTTCAAACTATCCCTCCTCGTTTGTACAAAGGGTTTCGTGCTCAGACACGCGCTGGTGAGTTCGATGTCAGGTTGTAG

Protein Analysis

415

Amino Acids

46.31

Weight (kDa)

5.4

Isoelectric Point (pI)

31.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 167 - 415 1.3e-74 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 549
AccII CGCG 2 cut(s) 72, 1221
AciI CCGC 2 cut(s) 488, 718
AclWI GGATC 4 cut(s) 75, 111, 512, 887
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 413
AcvI CACGTG 1 cut(s) 1096
AfaI GTAC 5 cut(s) 281, 527, 856, 1009, 1196
AfeI AGCGCT 1 cut(s) 1068
AfiI CCNNNNNNNGG 1 cut(s) 767
AflIII ACRYGT 3 cut(s) 857, 1004, 1095
AgsI TTSAA 7 cut(s) 8, 176, 221, 560, 778, 1111, 1175
AjnI CCWGG 2 cut(s) 895, 1075
AleI CACNNNNGTG 1 cut(s) 711
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 8 cut(s) 58, 206, 337, 407, 640, 781, 805, 934
AluI AGCT 8 cut(s) 58, 206, 337, 407, 640, 781, 805, 934
Alw21I GWGCWC 1 cut(s) 1213
AlwI GGATC 4 cut(s) 75, 111, 512, 887
Ama87I CYCGRG 1 cut(s) 1115
Aor51HI AGCGCT 1 cut(s) 1068
AoxI GGCC 1 cut(s) 1147
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 1 cut(s) 631
ArsI GACNNNNNNTTYG 2 cut(s) 531, 563
Asp700I GAANNNNTTC 2 cut(s) 454, 1089
AspLEI GCGC 2 cut(s) 1069, 1223
AsuHPI GGTGA 4 cut(s) 147, 698, 808, 1238
AsuII TTCGAA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 494
BbrPI CACGTG 1 cut(s) 1096
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 1213
BbvI GCAGC 1 cut(s) 67
BccI CCATC 5 cut(s) 229, 559, 581, 688, 761
BceAI ACGGC 1 cut(s) 1103
BciT130I CCWGG 2 cut(s) 897, 1077
BciVI GTATCC 1 cut(s) 398
BfaI CTAG 7 cut(s) 47, 384, 419, 531, 689, 875, 1064
BfmI CTRYAG 2 cut(s) 721, 954
BfoI RGCGCY 1 cut(s) 1070
BfuI GTATCC 1 cut(s) 398
BglII AGATCT 2 cut(s) 301, 958
BisI GCNGC 1 cut(s) 56
BlsI GCNGC 1 cut(s) 57
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 897, 1077
BmeT110I CYCGRG 1 cut(s) 1115
BmiI GGNNCC 1 cut(s) 1074
BmrFI CCNGG 2 cut(s) 897, 1077
BmsI GCATC 1 cut(s) 559
BpiI GAAGAC 1 cut(s) 699
BpmI CTGGAG 2 cut(s) 54, 747
Bpu14I TTCGAA 1 cut(s) 458
BpuEI CTTGAG 2 cut(s) 102, 943
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 1096
BsaBI GATNNNNATC 1 cut(s) 239
BsaJI CCNNGG 5 cut(s) 283, 503, 728, 896, 1076
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 1168, 1198
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 3 cut(s) 67, 143, 649
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 239
BseBI CCWGG 2 cut(s) 897, 1077
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 5 cut(s) 283, 503, 728, 896, 1076
BseGI GGATG 3 cut(s) 349, 574, 592
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 3 cut(s) 267, 650, 1226
BseNI ACTGG 3 cut(s) 67, 143, 649
BseRI GAGGAG 1 cut(s) 1176
BseXI GCAGC 1 cut(s) 67
Bsh1236I CGCG 2 cut(s) 72, 1221
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 1 cut(s) 1149
BshVI ATCGAT 1 cut(s) 354
BsiEI CGRYCG 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 1213
BsiHKCI CYCGRG 1 cut(s) 1115
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 1 cut(s) 1149
BsoBI CYCGRG 1 cut(s) 1115
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 494, 1213
Bsp1407I TGTACA 2 cut(s) 1007, 1194
Bsp143I GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
Bsp19I CCATGG 1 cut(s) 728
BspACI CCGC 2 cut(s) 488, 718
BspANI GGCC 1 cut(s) 1149
BspCNI CTCAG 3 cut(s) 268, 649, 1225
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 2 cut(s) 72, 1221
BspLI GGNNCC 1 cut(s) 1074
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 4 cut(s) 75, 111, 512, 887
BspT104I TTCGAA 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 1134
BsrGI TGTACA 2 cut(s) 1007, 1194
BsrI ACTGG 3 cut(s) 67, 143, 649
BssECI CCNNGG 5 cut(s) 283, 503, 728, 896, 1076
BssMI GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
BssT1I CCWWGG 3 cut(s) 283, 503, 728
Bst2UI CCWGG 2 cut(s) 897, 1077
Bst4CI ACNGT 3 cut(s) 231, 944, 982
Bst6I CTCTTC 1 cut(s) 555
BstAUI TGTACA 2 cut(s) 1007, 1194
BstBAI YACGTR 1 cut(s) 1096
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 490, 872
BstDEI CTNAG 5 cut(s) 202, 276, 636, 1151, 1212
BstDSI CCRYGG 1 cut(s) 728
BstF5I GGATG 3 cut(s) 349, 574, 592
BstFNI CGCG 2 cut(s) 72, 1221
BstH2I RGCGCY 1 cut(s) 1070
BstHHI GCGC 2 cut(s) 1069, 1223
BstKTI GATC 8 cut(s) 83, 106, 304, 354, 520, 895, 961, 1171
BstMBI GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstNI CCWGG 2 cut(s) 897, 1077
BstNSI RCATGY 2 cut(s) 861, 1008
BstSCI CCNGG 2 cut(s) 895, 1075
BstSFI CTRYAG 2 cut(s) 721, 954
BstUI CGCG 2 cut(s) 72, 1221
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 4 cut(s) 80, 301, 517, 958
BstYI RGATCY 4 cut(s) 80, 301, 517, 958
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 1 cut(s) 1149
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 728
BtgZI GCGATG 1 cut(s) 53
BtsCI GGATG 3 cut(s) 349, 574, 592
BtsIMutI CAGTG 3 cut(s) 150, 642, 940
Cac8I GCNNGC 2 cut(s) 490, 872
CfoI GCGC 2 cut(s) 1069, 1223
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 5 cut(s) 280, 526, 855, 1008, 1195
CviAII CATG 6 cut(s) 697, 729, 858, 974, 1005, 1011
CviQI GTAC 5 cut(s) 280, 526, 855, 1008, 1195
DdeI CTNAG 5 cut(s) 202, 276, 636, 1151, 1212
DpnI GATC 8 cut(s) 82, 105, 303, 353, 519, 894, 960, 1170
DpnII GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco130I CCWWGG 3 cut(s) 283, 503, 728
Eco24I GRGCYC 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 1068
Eco57I CTGAAG 1 cut(s) 413
Eco72I CACGTG 1 cut(s) 1096
Eco88I CYCGRG 1 cut(s) 1115
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 895, 1075
EcoT14I CCWWGG 3 cut(s) 283, 503, 728
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 3 cut(s) 283, 503, 728
FaeI CATG 6 cut(s) 700, 732, 861, 977, 1008, 1014
FaqI GGGAC 1 cut(s) 743
FatI CATG 6 cut(s) 696, 728, 857, 973, 1004, 1010
FauI CCCGC 1 cut(s) 481
FauNDI CATATG 1 cut(s) 86
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 56
FokI GGATG 3 cut(s) 356, 581, 599
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 1 cut(s) 56
FspBI CTAG 7 cut(s) 47, 384, 419, 531, 689, 875, 1064
GlaI GCGC 2 cut(s) 1068, 1222
GluI GCNGC 1 cut(s) 56
GsuI CTGGAG 2 cut(s) 54, 747
HaeII RGCGCY 1 cut(s) 1070
HaeIII GGCC 1 cut(s) 1149
HhaI GCGC 2 cut(s) 1069, 1223
Hin1II CATG 6 cut(s) 700, 732, 861, 977, 1008, 1014
Hin6I GCGC 2 cut(s) 1067, 1221
HinP1I GCGC 2 cut(s) 1067, 1221
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 779
HphI GGTGA 4 cut(s) 147, 698, 808, 1238
Hpy166II GTNNAC 2 cut(s) 550, 580
Hpy188I TCNGA 7 cut(s) 28, 277, 480, 847, 887, 1104, 1215
Hpy188III TCNNGA 7 cut(s) 33, 185, 391, 653, 764, 1056, 1159
Hpy8I GTNNAC 2 cut(s) 550, 580
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 3 cut(s) 231, 944, 982
HpyCH4IV ACGT 2 cut(s) 197, 1095
HpyCH4V TGCA 3 cut(s) 255, 572, 956
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 5 cut(s) 202, 276, 636, 1151, 1212
HpySE526I ACGT 2 cut(s) 197, 1095
Hsp92II CATG 6 cut(s) 700, 732, 861, 977, 1008, 1014
HspAI GCGC 2 cut(s) 1067, 1221
Kzo9I GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 559
MaeI CTAG 7 cut(s) 47, 384, 419, 531, 689, 875, 1064
MaeII ACGT 2 cut(s) 197, 1095
MaeIII GTNAC 5 cut(s) 185, 442, 467, 788, 982
MalI GATC 8 cut(s) 82, 105, 303, 353, 519, 894, 960, 1170
MboI GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
MboII GAAGA 5 cut(s) 311, 572, 704, 811, 1163
MfeI CAATTG 2 cut(s) 573, 906
MflI RGATCY 4 cut(s) 80, 301, 517, 958
MhlI GDGCHC 2 cut(s) 494, 1213
MluCI AATT 8 cut(s) 177, 192, 316, 573, 593, 631, 864, 906
MlyI GAGTC 1 cut(s) 920
MmeI TCCRAC 1 cut(s) 1127
MnlI CCTC 9 cut(s) 31, 378, 693, 771, 843, 948, 1001, 1194, 1197
MroXI GAANNNNTTC 2 cut(s) 454, 1089
MseI TTAA 3 cut(s) 132, 309, 1122
MslI CAYNNNNRTG 2 cut(s) 711, 1009
MspR9I CCNGG 2 cut(s) 897, 1077
MunI CAATTG 2 cut(s) 573, 906
MvaI CCWGG 2 cut(s) 897, 1077
MvnI CGCG 2 cut(s) 72, 1221
MwoI GCNNNNNNNGC 1 cut(s) 52
NcoI CCATGG 1 cut(s) 728
NdeI CATATG 1 cut(s) 86
NdeII GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
NlaIII CATG 6 cut(s) 700, 732, 861, 977, 1008, 1014
NlaIV GGNNCC 1 cut(s) 1074
NmuCI GTSAC 2 cut(s) 185, 467
NspI RCATGY 2 cut(s) 861, 1008
NspV TTCGAA 1 cut(s) 458
OliI CACNNNNGTG 1 cut(s) 711
PciI ACATGT 2 cut(s) 857, 1004
PdmI GAANNNNTTC 2 cut(s) 454, 1089
PfeI GAWTC 9 cut(s) 29, 125, 415, 676, 734, 848, 882, 1026, 1155
PflFI GACNNNGTC 1 cut(s) 664
PkrI GCNGC 1 cut(s) 57
PleI GAGTC 1 cut(s) 919
PmaCI CACGTG 1 cut(s) 1096
PmlI CACGTG 1 cut(s) 1096
PpsI GAGTC 1 cut(s) 919
Ppu21I YACGTR 1 cut(s) 1096
PscI ACATGT 2 cut(s) 857, 1004
Psp6I CCWGG 2 cut(s) 895, 1075
PspCI CACGTG 1 cut(s) 1096
PspGI CCWGG 2 cut(s) 895, 1075
PspN4I GGNNCC 1 cut(s) 1074
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 4 cut(s) 80, 301, 517, 958
PsyI GACNNNGTC 1 cut(s) 664
RsaI GTAC 5 cut(s) 281, 527, 856, 1009, 1196
RsaNI GTAC 5 cut(s) 280, 526, 855, 1008, 1195
RseI CAYNNNNRTG 2 cut(s) 711, 1009
SalI GTCGAC 1 cut(s) 548
SaqAI TTAA 3 cut(s) 132, 309, 1122
SatI GCNGC 1 cut(s) 56
Sau3AI GATC 8 cut(s) 80, 103, 301, 351, 517, 892, 958, 1168
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 1 cut(s) 920
ScrFI CCNGG 2 cut(s) 897, 1077
SduI GDGCHC 2 cut(s) 494, 1213
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 2 cut(s) 721, 954
SfuI TTCGAA 1 cut(s) 458
SmiMI CAYNNNNRTG 2 cut(s) 711, 1009
SmlI CTYRAG 2 cut(s) 117, 922
SmoI CTYRAG 2 cut(s) 117, 922
Sse9I AATT 8 cut(s) 177, 192, 316, 573, 593, 631, 864, 906
SsiI CCGC 2 cut(s) 488, 718
SspMI CTAG 7 cut(s) 47, 384, 419, 531, 689, 875, 1064
StyD4I CCNGG 2 cut(s) 895, 1075
StyI CCWWGG 3 cut(s) 283, 503, 728
TaaI ACNGT 3 cut(s) 231, 944, 982
TaiI ACGT 2 cut(s) 200, 1098
TaqI TCGA 8 cut(s) 243, 354, 458, 549, 862, 914, 1158, 1233
TaqII GACCGA 1 cut(s) 526
TasI AATT 8 cut(s) 177, 192, 316, 573, 593, 631, 864, 906
TatI WGTACW 3 cut(s) 525, 1007, 1194
TfiI GAWTC 9 cut(s) 29, 125, 415, 676, 734, 848, 882, 1026, 1155
Tru1I TTAA 3 cut(s) 132, 309, 1122
Tru9I TTAA 3 cut(s) 132, 309, 1122
TscAI CASTG 3 cut(s) 150, 649, 947
TseFI GTSAC 2 cut(s) 185, 467
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 2 cut(s) 185, 467
TspDTI ATGAA 3 cut(s) 310, 312, 644
TspGWI ACGGA 2 cut(s) 204, 1035
TspRI CASTG 3 cut(s) 150, 649, 947
Tth111I GACNNNGTC 1 cut(s) 664
XapI RAATTY 1 cut(s) 631
XceI RCATGY 2 cut(s) 861, 1008
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 2 cut(s) 454, 1089
XspI CTAG 7 cut(s) 47, 384, 419, 531, 689, 875, 1064
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.