RchiOBHm_Chr3g0457601

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009630
Physical Location & Seq
Reverse (-)
6653087 .. 6657289
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42435

Sequence Viewer

Length: 1248 bp
ATGTTGCAGTGCATATTTCTGCTATCTGATTCGGGTGAGGTAATGCTGGAGAAACAGCTAACTGGGCAGCGTGTAGACCGTTCGATATGTGTATGGTTCTGGGAACAAGCCATTTCTCAAGGTGATTCCTCCAAGCTGCAACCAGTAATTGCTTCACCAACACATTACCTTTTCCAAATTCTTCGCGAGGGGATAACATTTTTAGCTTGTACCCAAGTTGAAATGCCACCTTTGATGGGCATTGAGTTCCTTTGCAGGGTAGCTGATGTCCTCACAGATTATCTTGGCAGCTTGAATGAAGACTTGATAAAAGATAACTTTGTTATCGTATATGAGCTTCTGGATGAGATGATTGACAATGGCTTCCCTCTCACTACAGAACCTAACATTCTGAGAGACATGATTGCTCCACCAAATCTTGTTAACAAGATGTTGAGTGTTGTGACAGGCAACAGTTCTAACATGAGTGATACACTTCCAGGTGCAACATCATCTCTGATTCCATGGAGAACAACAGATCCGAAATATGCTCACAATGAAGTTTATGTTGATCTTGTTGAAGAAATAGATACAATAATAAACAGGGATGGGGTCCTGGTGAAATGCGAGATATATGGTGAAGTTCAAGTGAACTCCCATTTATCAGGTGTACCTGATTTGACTCTTTCCTTTTCAAACCCTTCTATTCTTGATGATGTAAGATTCCATCCATGTGTTCGGTTTCGGCCTTGGGAATCCCAGCAAATTCTGTCGTTTGTGCCTCCTGATGGACAGTTCAAGCTAATGAGTTACAGGGTTAGAAAGTTGAAAAGCTCTCCAATCTATGTAAAGCCACAGCTAACCTCAGATGCTGGAACATGTCGTCTTAGTGTGATGGTTGGAATACGAAATGATCCTGGAAAGACCATTGATTCAATAACTGTGCAATTTCAACTGCCTCCATGCATTTTATCAGCTGATCTGACTTCAAATCATGGAACAGTAAACATTCTTTCAAATAAGACCTGTTCATGGTCAATTGGGCGTATTCCTAAAGATAAAACCCCTTCAATGTCTGGAACATTAGTGCTTGAGAAAGGATTAGAGCGCCTTCATGTGTTCCCCACATTTCAAGCAAGTTTTAGGATCATGGGTGTTGCTCTCTCTGGCTTGCAGATAGATAAGCTCGATTTGAGGAATCTACCAAATCGACCGTACAAGGGCTTTCGAGCACTCACTAAAGCAGGGCAATTTGAAGTTAGGTCGTAA

Protein Analysis

415

Amino Acids

46.43

Weight (kDa)

5.62

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 167 - 414 5.9e-71 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 186
AclWI GGATC 3 cut(s) 512, 887, 1133
AcsI RAATTY 2 cut(s) 177, 744
AfaI GTAC 3 cut(s) 211, 651, 1196
AfiI CCNNNNNNNGG 5 cut(s) 236, 256, 767, 1011, 1199
AflIII ACRYGT 1 cut(s) 857
AjnI CCWGG 3 cut(s) 478, 594, 895
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
Alw21I GWGCWC 1 cut(s) 1213
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 3 cut(s) 512, 887, 1133
AlwNI CAGNNNCTG 1 cut(s) 851
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 3 cut(s) 67, 136, 288
ApoI RAATTY 2 cut(s) 177, 744
AspLEI GCGC 1 cut(s) 1089
AspS9I GGNCC 1 cut(s) 592
AsuHPI GGTGA 5 cut(s) 47, 134, 147, 610, 629
AvaII GGWCC 1 cut(s) 592
BarI GAAGNNNNNNTAC 2 cut(s) 321, 353
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 1213
BbvI GCAGC 3 cut(s) 79, 123, 300
BccI CCATC 5 cut(s) 229, 581, 714, 761, 868
BciT130I CCWGG 3 cut(s) 480, 596, 897
BcoDI GTCTC 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 1090
BisI GCNGC 3 cut(s) 68, 137, 289
BlsI GCNGC 3 cut(s) 69, 138, 290
Bme1390I CCNGG 3 cut(s) 480, 596, 897
Bme18I GGWCC 1 cut(s) 592
BmgT120I GGNCC 1 cut(s) 592
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 3 cut(s) 480, 596, 897
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 838
BmuI ACTGGG 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 102, 1091
BsaJI CCNNGG 2 cut(s) 503, 728
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 256, 767, 1011, 1199
Bse1I ACTGG 2 cut(s) 67, 143
BseBI CCWGG 3 cut(s) 480, 596, 897
BseDI CCNNGG 2 cut(s) 503, 728
BseGI GGATG 3 cut(s) 349, 592, 706
BseLI CCNNNNNNNGG 5 cut(s) 236, 256, 767, 1011, 1199
BseMII CTCAG 2 cut(s) 383, 858
BseNI ACTGG 2 cut(s) 67, 143
BseXI GCAGC 3 cut(s) 79, 123, 300
BseYI CCCAGC 1 cut(s) 738
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 1193
BshFI GGCC 1 cut(s) 727
BsiEI CGRYCG 1 cut(s) 1193
BsiHKAI GWGCWC 1 cut(s) 1213
BslI CCNNNNNNNGG 5 cut(s) 236, 256, 767, 1011, 1199
BsmAI GTCTC 1 cut(s) 390
BsnI GGCC 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 1213
Bsp143I GATC 5 cut(s) 517, 550, 892, 958, 1125
Bsp19I CCATGG 1 cut(s) 503
Bsp68I TCGCGA 1 cut(s) 186
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 2 cut(s) 384, 857
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 593
BspPI GGATC 3 cut(s) 512, 887, 1133
BsrI ACTGG 2 cut(s) 67, 143
BssECI CCNNGG 2 cut(s) 503, 728
BssMI GATC 5 cut(s) 517, 550, 892, 958, 1125
BssT1I CCWWGG 2 cut(s) 503, 728
Bst2UI CCWGG 3 cut(s) 480, 596, 897
Bst4CI ACNGT 6 cut(s) 80, 455, 774, 922, 982, 1194
BstC8I GCNNGC 1 cut(s) 1151
BstDEI CTNAG 3 cut(s) 392, 844, 866
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 3 cut(s) 349, 592, 706
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 1090
BstHHI GCGC 1 cut(s) 1089
BstKTI GATC 5 cut(s) 520, 553, 895, 961, 1128
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 5 cut(s) 517, 550, 892, 958, 1125
BstMCI CGRYCG 1 cut(s) 1193
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 3 cut(s) 480, 596, 897
BstNSI RCATGY 1 cut(s) 861
BstSCI CCNGG 3 cut(s) 478, 594, 895
BstSFI CTRYAG 1 cut(s) 375
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 79, 123, 300
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 3 cut(s) 349, 592, 706
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 14
BtuMI TCGCGA 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 1151
CaiI CAGNNNCTG 1 cut(s) 851
CfoI GCGC 1 cut(s) 1089
Cfr13I GGNCC 1 cut(s) 592
Csp6I GTAC 3 cut(s) 210, 650, 1195
CviQI GTAC 3 cut(s) 210, 650, 1195
DdeI CTNAG 3 cut(s) 392, 844, 866
DpnI GATC 5 cut(s) 519, 552, 894, 960, 1127
DpnII GATC 5 cut(s) 517, 550, 892, 958, 1125
Eco130I CCWWGG 2 cut(s) 503, 728
Eco47I GGWCC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 3 cut(s) 478, 594, 895
EcoT14I CCWWGG 2 cut(s) 503, 728
EcoT22I ATGCAT 1 cut(s) 947
ErhI CCWWGG 2 cut(s) 503, 728
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 3 cut(s) 68, 137, 289
FokI GGATG 3 cut(s) 356, 599, 693
Fsp4HI GCNGC 3 cut(s) 68, 137, 289
GlaI GCGC 1 cut(s) 1088
GluI GCNGC 3 cut(s) 68, 137, 289
GsaI CCCAGC 1 cut(s) 742
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 1090
HaeIII GGCC 1 cut(s) 727
HhaI GCGC 1 cut(s) 1089
Hin6I GCGC 1 cut(s) 1087
HinP1I GCGC 1 cut(s) 1087
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 8 cut(s) 29, 125, 499, 661, 702, 734, 911, 1177
HpaI GTTAAC 1 cut(s) 424
HphI GGTGA 5 cut(s) 47, 134, 147, 610, 629
Hpy166II GTNNAC 5 cut(s) 76, 424, 631, 650, 985
Hpy188I TCNGA 6 cut(s) 28, 393, 498, 522, 847, 963
Hpy188III TCNNGA 5 cut(s) 185, 341, 689, 764, 1056
Hpy8I GTNNAC 5 cut(s) 76, 424, 631, 650, 985
HpyAV CCTTC 3 cut(s) 690, 1056, 1100
HpyCH4III ACNGT 6 cut(s) 80, 455, 774, 922, 982, 1194
HpyCH4V TGCA 8 cut(s) 7, 12, 139, 255, 485, 925, 945, 1153
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 3 cut(s) 392, 844, 866
HspAI GCGC 1 cut(s) 1087
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 5 cut(s) 517, 550, 892, 958, 1125
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 3 cut(s) 79, 123, 300
LweI GCATC 1 cut(s) 838
MaeIII GTNAC 2 cut(s) 442, 788
MalI GATC 5 cut(s) 519, 552, 894, 960, 1127
MboI GATC 5 cut(s) 517, 550, 892, 958, 1125
MboII GAAGA 3 cut(s) 173, 311, 572
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 1213
MluCI AATT 6 cut(s) 147, 177, 744, 926, 1017, 1229
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 859
MnlI CCTC 9 cut(s) 31, 139, 181, 281, 378, 771, 853, 948, 1167
Mph1103I ATGCAT 1 cut(s) 947
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 1 cut(s) 956
MspR9I CCNGG 3 cut(s) 480, 596, 897
MunI CAATTG 1 cut(s) 1017
MvaI CCWGG 3 cut(s) 480, 596, 897
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 64
NcoI CCATGG 1 cut(s) 503
NdeII GATC 5 cut(s) 517, 550, 892, 958, 1125
NlaIV GGNNCC 1 cut(s) 593
NmuCI GTSAC 1 cut(s) 442
NruI TCGCGA 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 947
NspI RCATGY 1 cut(s) 861
PciI ACATGT 1 cut(s) 857
PfeI GAWTC 7 cut(s) 29, 125, 499, 702, 734, 911, 1177
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 3 cut(s) 69, 138, 290
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PpuMI RGGWCCY 1 cut(s) 592
PscI ACATGT 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 3 cut(s) 478, 594, 895
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 3 cut(s) 478, 594, 895
PspN4I GGNNCC 1 cut(s) 593
PspPI GGNCC 1 cut(s) 592
PspPPI RGGWCCY 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 956
RruI TCGCGA 1 cut(s) 186
RsaI GTAC 3 cut(s) 211, 651, 1196
RsaNI GTAC 3 cut(s) 210, 650, 1195
RseI CAYNNNNRTG 1 cut(s) 711
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 3 cut(s) 68, 137, 289
Sau3AI GATC 5 cut(s) 517, 550, 892, 958, 1125
Sau96I GGNCC 1 cut(s) 592
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 3 cut(s) 480, 596, 897
SduI GDGCHC 1 cut(s) 1213
SfaNI GCATC 1 cut(s) 838
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 2 cut(s) 117, 1070
SmoI CTYRAG 2 cut(s) 117, 1070
Sse9I AATT 6 cut(s) 147, 177, 744, 926, 1017, 1229
StyD4I CCNGG 3 cut(s) 478, 594, 895
StyI CCWWGG 2 cut(s) 503, 728
TaaI ACNGT 6 cut(s) 80, 455, 774, 922, 982, 1194
TaqI TCGA 4 cut(s) 83, 1167, 1189, 1207
TasI AATT 6 cut(s) 147, 177, 744, 926, 1017, 1229
TfiI GAWTC 7 cut(s) 29, 125, 499, 702, 734, 911, 1177
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 67, 136, 288
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 4 cut(s) 312, 552, 999, 1082
TspRI CASTG 1 cut(s) 14
VpaK11BI GGWCC 1 cut(s) 592
XapI RAATTY 2 cut(s) 177, 744
XceI RCATGY 1 cut(s) 861
XmiI GTMKAC 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.