AT1G57850

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0018301
Physical Location & Seq
Forward (+)
21425481 .. 21428078
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G57850.1

Sequence Viewer

Length: 579 bp
ATGACGATTTTGTCTCTCGCCAATGACGACTTCCATCGAGTATTCCTCAGTTTCCGAGGAGATCAGTTGCGCTACAGCTTTGTCAGCCACCTTCTTGACGCCTTTGAGAGACATGGAATCTTGTGTTTTGTAGACAAAGACGAGCTAAGGGGAAAAACCATGACAAACCTTTTTGTAAGGATTAAAGAGTCGAAGATCGCACTAGTTATCTTCTCTAGTAGGTATGCGGAGTCGAGTTGGTGCATGGATGAGCTGGTGAAGATGAAGAAACGTGCGGATAAAGGAAAGCTCCAAGTCATTCCCATCTTCTACAAGGTTAGGGCAAGAGATGTGAGAGGACAGACCGGTGAGTTTGGTGAAACGTTTTGGGCGCTTGCAAGAACTTCTCGAGGAGATCAGATCATGGAGTGGAAGGAAGCGCTAGAGTGTATTTCCAACAAGATGGGCCTGTCCTTGGGAGACAAAAGTTCAGAAGCTGATTTTATCAAGGAAATTGTTAAAGAGGTTAAGAGAGTTGTAGCCGAAATTGGATGCCAGGCCACAGAAAACCATCAATGTATCAATTTTCTCCCGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR

Protein Analysis

192

Amino Acids

22.12

Weight (kDa)

8.57

Isoelectric Point (pI)

26.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 172 1.7e-41 TIR domain
TIR_2 PF13676 14 - 107 1.5e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09665 AT1G47370 AT1G57670 AT1G57830 AT1G57850 AT1G57850 AT1G57850 AT1G60320 AT2G03030 AT2G03300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccI GTMKAC 1 cut(s) 132
AciI CCGC 2 cut(s) 227, 275
AclI AACGTT 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 99
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 344
AhlI ACTAGT 1 cut(s) 202
AjnI CCWGG 1 cut(s) 534
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 5 cut(s) 78, 145, 253, 289, 476
AluI AGCT 5 cut(s) 78, 145, 253, 289, 476
Alw26I GTCTC 3 cut(s) 18, 103, 453
AlwNI CAGNNNCTG 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 387
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 2 cut(s) 445, 537
AsiGI ACCGGT 1 cut(s) 344
AspLEI GCGC 3 cut(s) 72, 373, 421
AspS9I GGNCC 1 cut(s) 445
AsuHPI GGTGA 3 cut(s) 268, 359, 368
AvaI CYCGRG 1 cut(s) 387
BccI CCATC 4 cut(s) 42, 311, 436, 558
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 18, 103, 453
BcuI ACTAGT 1 cut(s) 202
BfaI CTAG 3 cut(s) 203, 216, 422
BfmI CTRYAG 1 cut(s) 73
BfoI RGCGCY 2 cut(s) 374, 422
Bme1390I CCNGG 1 cut(s) 536
BmeT110I CYCGRG 1 cut(s) 387
BmgT120I GGNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 521
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bpu10I CCTNAGC 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 55, 453
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse118I RCCGGY 1 cut(s) 344
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 55, 453
BseGI GGATG 2 cut(s) 253, 536
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 61
BseRI GAGGAG 2 cut(s) 72, 405
BshFI GGCC 2 cut(s) 447, 539
BshTI ACCGGT 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 3 cut(s) 18, 103, 453
BsnI GGCC 2 cut(s) 447, 539
BsoBI CYCGRG 1 cut(s) 387
Bsp143I GATC 4 cut(s) 61, 195, 394, 399
BspACI CCGC 2 cut(s) 227, 275
BspANI GGCC 2 cut(s) 447, 539
BspCNI CTCAG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 55, 453
BssMI GATC 4 cut(s) 61, 195, 394, 399
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 536
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 47, 146
BstF5I GGATG 2 cut(s) 253, 536
BstH2I RGCGCY 2 cut(s) 374, 422
BstHHI GCGC 3 cut(s) 72, 373, 421
BstKTI GATC 4 cut(s) 64, 198, 397, 402
BstMAI GTCTC 3 cut(s) 18, 103, 453
BstMBI GATC 4 cut(s) 61, 195, 394, 399
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstSFI CTRYAG 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 442
BsuRI GGCC 2 cut(s) 447, 539
BtsCI GGATG 2 cut(s) 253, 536
Cac8I GCNNGC 1 cut(s) 375
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 3 cut(s) 72, 373, 421
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 445
CseI GACGC 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 344
CviAII CATG 4 cut(s) 113, 160, 244, 403
CviJI RGCY 9 cut(s) 78, 87, 145, 253, 289, 447, 476, 521, 539
CviKI_1 RGCY 9 cut(s) 78, 87, 145, 253, 289, 447, 476, 521, 539
DdeI CTNAG 2 cut(s) 47, 146
DpnI GATC 4 cut(s) 63, 197, 396, 401
DpnII GATC 4 cut(s) 61, 195, 394, 399
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 453
Eco47III AGCGCT 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 4 cut(s) 116, 163, 247, 406
FaiI YATR 5 cut(s) 114, 161, 225, 245, 404
FatI CATG 4 cut(s) 112, 159, 243, 402
FblI GTMKAC 1 cut(s) 132
FokI GGATG 2 cut(s) 260, 543
FspBI CTAG 3 cut(s) 203, 216, 422
GlaI GCGC 3 cut(s) 71, 372, 420
HaeII RGCGCY 2 cut(s) 374, 422
HaeIII GGCC 2 cut(s) 447, 539
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 107
HhaI GCGC 3 cut(s) 72, 373, 421
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 4 cut(s) 116, 163, 247, 406
Hin6I GCGC 3 cut(s) 70, 371, 419
HinP1I GCGC 3 cut(s) 70, 371, 419
HinfI GANTC 3 cut(s) 117, 188, 230
HpaII CCGG 1 cut(s) 345
HphI GGTGA 3 cut(s) 268, 359, 368
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 3 cut(s) 56, 399, 472
Hpy188III TCNNGA 3 cut(s) 95, 387, 571
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 2 cut(s) 101, 406
HpyCH4IV ACGT 2 cut(s) 271, 362
HpyCH4V TGCA 2 cut(s) 243, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpyF3I CTNAG 2 cut(s) 47, 146
HpySE526I ACGT 2 cut(s) 271, 362
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 4 cut(s) 116, 163, 247, 406
HspAI GCGC 3 cut(s) 70, 371, 419
Kzo9I GATC 4 cut(s) 61, 195, 394, 399
LmnI GCTCC 1 cut(s) 294
LpnPI CCDG 5 cut(s) 239, 358, 461, 521, 548
LweI GCATC 1 cut(s) 521
MaeI CTAG 3 cut(s) 203, 216, 422
MaeII ACGT 2 cut(s) 271, 362
MalI GATC 4 cut(s) 63, 197, 396, 401
MboI GATC 4 cut(s) 61, 195, 394, 399
MboII GAAGA 5 cut(s) 202, 205, 271, 277, 298
MluCI AATT 4 cut(s) 492, 525, 562, 574
MlyI GAGTC 2 cut(s) 197, 239
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 5 cut(s) 50, 56, 329, 383, 496
MseI TTAA 3 cut(s) 183, 498, 507
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 84
NdeII GATC 4 cut(s) 61, 195, 394, 399
NlaIII CATG 4 cut(s) 116, 163, 247, 406
PaeR7I CTCGAG 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 344
PleI GAGTC 2 cut(s) 196, 238
PpsI GAGTC 2 cut(s) 196, 238
Psp1406I AACGTT 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspPI GGNCC 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 476
SaqAI TTAA 3 cut(s) 183, 498, 507
Sau3AI GATC 4 cut(s) 61, 195, 394, 399
Sau96I GGNCC 1 cut(s) 445
SchI GAGTC 2 cut(s) 197, 239
ScrFI CCNGG 1 cut(s) 536
SfaNI GCATC 1 cut(s) 521
SfcI CTRYAG 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 387
SlaI CTCGAG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
SpeI ACTAGT 1 cut(s) 202
Sse9I AATT 4 cut(s) 492, 525, 562, 574
SsiI CCGC 2 cut(s) 227, 275
SspMI CTAG 3 cut(s) 203, 216, 422
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 2 cut(s) 274, 365
TaqI TCGA 4 cut(s) 37, 191, 233, 388
TasI AATT 4 cut(s) 492, 525, 562, 574
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 3 cut(s) 183, 498, 507
Tru9I TTAA 3 cut(s) 183, 498, 507
TspDTI ATGAA 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 3 cut(s) 203, 216, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.