AT2G03300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0018301
Physical Location & Seq
Reverse (-)
1003468 .. 1004222
755 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03300.1

Sequence Viewer

Length: 612 bp
ATGACATTCTTCTCTCCCACTCAGGTGTTTTTGAACTACAGGGGAGAACAACTGCGTCGCAGCTTCGTGAGCCACCTCATTGATGCCTTTGAAAGGAATGAGATCAACTTCTTCGTAGACAAATACGAACAGAGAGGCAAAGACCTCAAAAATCTCTTTCTTAGGATCCAAGAGTCGAAGATCGCGCTTGCCATCTTCTCAACCAGATACACGGAGTCAAGCTGGTGTATGGATGAGTTGGTGAAGATAAAGAAACTTGCTGATAAAAGAAAGCTCCATGTCATTCCAATTTTCTACAAGGTGAAGGTAGAAGACGTTCGAAAACAGACAGGTGAGTTTGGTGACAACTTCTGGACGCTGGCAAAGGTTTCAAGTGGTGATCAGATCAAGAAATGGAAAGAAGCCTTGGAATGTATCCCCAACAAGATGGGTTTGTCGTTGGGAGACAAGAGTGATGAAGCAGATTTCATCAAGGAAGTTGTTAAGGCGGTTCAGTGTGTTGTAGCAACGATTGGACTTGAGGAAGAAGAGAATCATTTTGGGAAAAAGAAGAGAAAGGATTGCAAATGTGAGCTTCCTGATTTGAAGAAAAGCAGAACCAAAAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

203

Amino Acids

23.74

Weight (kDa)

9.23

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 8 - 181 1e-41 TIR domain
TIR_2 PF13676 9 - 102 1.1e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09665 AT1G47370 AT1G57670 AT1G57830 AT1G57850 AT1G57850 AT1G57850 AT1G60320 AT2G03030 AT2G03300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 185
AciI CCGC 1 cut(s) 488
AclWI GGATC 2 cut(s) 160, 173
AfiI CCNNNNNNNGG 1 cut(s) 93
AgsI TTSAA 4 cut(s) 34, 92, 372, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AleI CACNNNNGTG 1 cut(s) 23
AluBI AGCT 4 cut(s) 63, 222, 274, 574
AluI AGCT 4 cut(s) 63, 222, 274, 574
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 2 cut(s) 160, 173
ApeKI GCWGC 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 187
AsuHPI GGTGA 5 cut(s) 253, 313, 344, 353, 389
AsuII TTCGAA 1 cut(s) 319
BamHI GGATCC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 72
BccI CCATC 2 cut(s) 200, 421
BciVI GTATCC 1 cut(s) 425
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 37
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 167
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 185
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 438
Bsp119I TTCGAA 1 cut(s) 319
Bsp143I GATC 5 cut(s) 102, 165, 180, 379, 384
BspACI CCGC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 34
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 1 cut(s) 167
BspPI GGATC 2 cut(s) 160, 173
BspT104I TTCGAA 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 5 cut(s) 102, 165, 180, 379, 384
BssT1I CCWWGG 1 cut(s) 405
Bst6I CTCTTC 2 cut(s) 522, 545
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 189, 360
BstDEI CTNAG 2 cut(s) 21, 161
BstENI CCTNNNNNAGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 5 cut(s) 105, 168, 183, 382, 387
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 5 cut(s) 102, 165, 180, 379, 384
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 165
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 425
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 189, 360
CfoI GCGC 1 cut(s) 187
CseI GACGC 2 cut(s) 44, 364
CviAII CATG 1 cut(s) 278
CviJI RGCY 6 cut(s) 63, 72, 222, 274, 404, 574
CviKI_1 RGCY 6 cut(s) 63, 72, 222, 274, 404, 574
DdeI CTNAG 2 cut(s) 21, 161
DpnI GATC 5 cut(s) 104, 167, 182, 381, 386
DpnII GATC 5 cut(s) 102, 165, 180, 379, 384
Eam1104I CTCTTC 2 cut(s) 522, 545
EarI CTCTTC 2 cut(s) 522, 545
Eco130I CCWWGG 1 cut(s) 405
EcoNI CCTNNNNNAGG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 1 cut(s) 281
FaiI YATR 2 cut(s) 230, 279
FatI CATG 1 cut(s) 277
FbaI TGATCA 1 cut(s) 379
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 1 cut(s) 61
GlaI GCGC 1 cut(s) 186
GluI GCNGC 1 cut(s) 61
HgaI GACGC 2 cut(s) 44, 364
HhaI GCGC 1 cut(s) 187
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 3 cut(s) 173, 215, 532
HphI GGTGA 5 cut(s) 253, 313, 344, 353, 389
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 1 cut(s) 384
Hpy188III TCNNGA 4 cut(s) 67, 352, 388, 578
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 1 cut(s) 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 2 cut(s) 21, 161
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 5 cut(s) 102, 165, 180, 379, 384
LmnI GCTCC 1 cut(s) 279
LpnPI CCDG 8 cut(s) 8, 25, 208, 217, 315, 337, 344, 591
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 1 cut(s) 73
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 341
MalI GATC 5 cut(s) 104, 167, 182, 381, 386
MboI GATC 5 cut(s) 102, 165, 180, 379, 384
MboII GAAGA 9 cut(s) 103, 187, 190, 256, 323, 536, 539, 562, 598
MflI RGATCY 1 cut(s) 165
MluCI AATT 1 cut(s) 288
MlyI GAGTC 2 cut(s) 182, 224
MnlI CCTC 4 cut(s) 86, 128, 155, 514
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 23
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 5 cut(s) 102, 165, 180, 379, 384
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 341
NspV TTCGAA 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 532
PkrI GCNGC 1 cut(s) 62
PleI GAGTC 2 cut(s) 181, 223
PpsI GAGTC 2 cut(s) 181, 223
PspN4I GGNNCC 1 cut(s) 167
PsrI GAACNNNNNNTAC 2 cut(s) 300, 332
PsuI RGATCY 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 5 cut(s) 102, 165, 180, 379, 384
SchI GAGTC 2 cut(s) 182, 224
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 1 cut(s) 288
SsiI CCGC 1 cut(s) 488
StyI CCWWGG 1 cut(s) 405
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 176, 319
TasI AATT 1 cut(s) 288
TfiI GAWTC 1 cut(s) 532
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 2 cut(s) 457, 471
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.