AT1G58520

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003874
Physical Location & Seq
Forward (+)
21729913 .. 21738808
8896 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58520.7

Sequence Viewer

Length: 2241 bp
ATGGAGATATCAGCTCTTCTTACATCTGCTGGAATTAATATTTCGATATGCATAGTTCTTTTATCACTTTACTCCATACTTAGGAAACAGCCAGCCAATTACTGTGTATATTTTGGCCGAAGGCTTGTTTGTGGTGGTGCTAGACGTTATGATCCTTTTTGGTATGAGAGGTTTGTGCCGTCTCCAAGTTGGCTTGTGAAAGCATGGGAGACTAGTGAAGACGAGTTATTAGCTGCTGCTGGTCTTGATGCTGTCGTCTTCCTTAGGATGGTCATTTTCAGCATTCGTATTTTCTTTATAGTTGCTGTTATTTGCATTGCCTTTGTGCTGCCTGTTAATTATTATGGGCAACCGATGGTGCATAAGGAAATCCATTTGGAGTCATCCGAAGTGTTTACAATTGAAAATCTTAAAGAAGGCTCAAAATGGCTGTGGGTTCATTGTCTTGCACTGTACATTATAACCTCAGCGGCATGTCTTCTGCTCTACTTTGAGTATAGCACCATAGCTAAAATGAGGCTTGGGCATATTACTGGATGTGCCTCAAAGCCAAGTCAATTTACCGTTCTTATCCGTGCTATTCCATGGTCTCCTGAGCAATCTTACAGCGACACGCTGAGCAAATTCTTCACAAACTACTACTCGTCCAGCTACGTGTCCCACCAAATGGTCTACCATAATGGCATCATTCAGAGACTGCTGCGTGATGCGGAGAGGATGTGCCAGACTCTAAAACACGTTTCTCCTGAAATCAATTGTAAACCGAGTTTAAGGCCATGCACCTTTTGTGGAGGACCTACAGCCACAAGTTCTTTTCATATCCTATCGAATGAGGCAGACAGTGTGAAAGGAATGGAGCTTGGTGAGTTGACTATGACTACGACTACGACAGAGCAAGAACGTTCAGCTGCTTTTGTGTTTTTTAAGACTCGCTATGATGCTCTTGTTGTTTCGGAGGTTCTACAATCATCAAATCCTATGTTATGGGTGACAGACTTAGCCCCAGAACCTCATGATGTGTACTGGAAGAATCTCAACATTCCTTATCGACAACTTTGGATACGGAAAATAGCAACCCTTGTTGGTGCAGTTGCTTTCATGTTTGTGTTTCTTATTCCCGTTACCTTCATTCAAGGGCTGACTCAACTAGTGCAGCTGTCTCACGCATTTCCTTTTCTAAGAGGCATCTTGAGCAAGAACTTTATAAACCAGGTCATCACAGGGTACTTACCCAGTGTGATCTTGATTCTATTTTTCTATGCCGTTCCACCATTGATGATGTATTTTTCGGCCTTGGAGGGATGTATTTCACGGAGTATAAGGAAGAAGAGTGCTTGCATCAAAGTCTTATATTTTACCATTTGGAATGTGTTCTTCGTGAATATATTATCAGGGTCTGTTATCAGGCAACTGAATGTCTTTTCTAGTGTCAGAGACATACCTGCACAACTTGCTAGAGCAGTGCCAACACAGGCTGGCTTCTTCATGACCTATTGTTTCACATCTGGTTGGGCCAGTTTGGCTTGTGAAATAATGCAGCCTATGGCTCTTATCTGGAATCTGGTAGCAAAAGTTGTTACCAAGAACGAGGATGAGTCATATGAAACACTTAGGTTCCCATACCACACCGAAATTCCTCGGCTGCTTTTGTTTGGGCTCCTGGGTTTCACCAATTCAGTCATAGCACCACTGATACTGCCGTTTTTGCTGATATACTTTTTCCTTGCATATCTCATATACAAAAATCAAATACTCAACGTGTATATTACGAAGTACGAAAGCGGTGGACAATACTGGCCTATTTTCCACAACACAACAATCTTTTCACTGATCTTGACACAGATTATAGCTTTGGGGTTTTTCGGATTAAAGCTATCAACAGTTGCTTCGGGTTTCACCATACCGTTAATTCTTCTCACGTTACTTTTTAGTGAGTATTGTCGGCAGAGGTTTGCGCCCATTTTCAACAAAAATCCTGCCCAGGTTCTCATAGATATGGACAGAGCTGATGAAATATCAGGAAAGATGGAAGAGTTACATAAAAAATTGCATAATGTATACTCGCAGATACCATTACACTCTCAGAAATCATCGAGTAAAGCTGAATGTAGTAATCCTTTCAAAAAGCAGGAGTTACCAGATCCTGAAAAATTGAAGCCAGAGGAGGGGGATGCGATAGCTAAAGAGTTATGGGGATTCCAAGGCAATGAATCTGGTCAAGAACATGACACTAAGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

746

Amino Acids

84.85

Weight (kDa)

8.49

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 165 7.5e-36 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 186 - 344 5.5e-26 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 355 - 621 1.3e-71 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 461, 1205
Acc36I ACCTGC 1 cut(s) 1450
AccB7I CCANNNNNTGG 1 cut(s) 667
AccI GTMKAC 2 cut(s) 672, 2058
AciI CCGC 3 cut(s) 470, 710, 1782
AclI AACGTT 1 cut(s) 901
AclWI GGATC 2 cut(s) 146, 2135
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 623, 1632
AfaI GTAC 4 cut(s) 455, 1022, 1226, 1775
AfiI CCNNNNNNNGG 4 cut(s) 81, 268, 667, 2165
AflIII ACRYGT 3 cut(s) 654, 736, 1758
AgsI TTSAA 5 cut(s) 404, 1133, 1966, 2122, 2155
AhdI GACNNNNNGTC 1 cut(s) 2233
AhlI ACTAGT 2 cut(s) 212, 1147
AjnI CCWGG 3 cut(s) 1209, 1659, 1980
Alw26I GTCTC 6 cut(s) 186, 203, 594, 688, 1164, 1428
AlwI GGATC 2 cut(s) 146, 2135
AlwNI CAGNNNCTG 3 cut(s) 697, 1397, 2144
AoxI GGCC 5 cut(s) 115, 773, 1290, 1512, 1796
ApeKI GCWGC 8 cut(s) 233, 236, 328, 700, 908, 1153, 1537, 1642
ApoI RAATTY 2 cut(s) 623, 1632
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 1370
AspLEI GCGC 1 cut(s) 1957
AspS9I GGNCC 2 cut(s) 794, 1512
AsuHPI GGTGA 4 cut(s) 875, 1000, 1660, 1888
AvaII GGWCC 1 cut(s) 794
AxyI CCTNAGG 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 1685, 1718
BanII GRGCYC 1 cut(s) 1659
BbsI GAAGAC 3 cut(s) 225, 250, 470
BbvCI CCTCAGC 1 cut(s) 466
BbvI GCAGC 8 cut(s) 220, 223, 315, 687, 895, 1165, 1549, 1629
BccI CCATC 3 cut(s) 262, 349, 2020
BceAI ACGGC 3 cut(s) 163, 1247, 1684
BciT130I CCWGG 3 cut(s) 1211, 1661, 1982
BciVI GTATCC 1 cut(s) 1053
BcoDI GTCTC 6 cut(s) 186, 203, 594, 688, 1164, 1428
BcuI ACTAGT 2 cut(s) 212, 1147
BfaI CTAG 5 cut(s) 141, 213, 1148, 1425, 1455
BfmI CTRYAG 1 cut(s) 798
BfuAI ACCTGC 1 cut(s) 1450
BfuI GTATCC 1 cut(s) 1053
BglI GCCNNNNNGGC 1 cut(s) 1520
BisI GCNGC 9 cut(s) 234, 237, 329, 471, 701, 909, 1154, 1538, 1643
BlpI GCTNAGC 1 cut(s) 617
BlsI GCNGC 9 cut(s) 235, 238, 330, 472, 702, 910, 1155, 1539, 1644
Bme1390I CCNGG 3 cut(s) 1211, 1661, 1982
Bme18I GGWCC 1 cut(s) 794
BmeRI GACNNNNNGTC 1 cut(s) 2233
BmgT120I GGNCC 2 cut(s) 794, 1512
BmiI GGNNCC 2 cut(s) 1616, 1658
BmrFI CCNGG 3 cut(s) 1211, 1661, 1982
BmrI ACTGGG 1 cut(s) 1227
BmsI GCATC 7 cut(s) 238, 693, 697, 928, 1194, 1347, 2161
BmuI ACTGGG 1 cut(s) 1227
BpiI GAAGAC 3 cut(s) 225, 250, 470
Bpu10I CCTNAGC 2 cut(s) 466, 594
Bpu1102I GCTNAGC 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 1210
BsaAI YACGTR 1 cut(s) 655
BsaI GGTCTC 1 cut(s) 594
BsaJI CCNNGG 6 cut(s) 584, 1293, 1637, 1660, 1980, 2200
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 268, 667, 2165
Bse1I ACTGG 5 cut(s) 538, 1028, 1233, 1515, 1799
Bse21I CCTNAGG 1 cut(s) 263
Bse3DI GCAATG 2 cut(s) 315, 2212
BseBI CCWGG 3 cut(s) 1211, 1661, 1982
BseDI CCNNGG 6 cut(s) 584, 1293, 1637, 1660, 1980, 2200
BseGI GGATG 7 cut(s) 273, 383, 542, 723, 1307, 1597, 2176
BseLI CCNNNNNNNGG 4 cut(s) 81, 268, 667, 2165
BseMI GCAATG 2 cut(s) 315, 2212
BseMII CTCAG 4 cut(s) 480, 585, 608, 2096
BseNI ACTGG 5 cut(s) 538, 1028, 1233, 1515, 1799
BseRI GAGGAG 1 cut(s) 2177
BseXI GCAGC 8 cut(s) 220, 223, 315, 687, 895, 1165, 1549, 1629
BsgI GTGCAG 3 cut(s) 1107, 1172, 1428
BshFI GGCC 5 cut(s) 117, 775, 1292, 1514, 1798
BslFI GGGAC 1 cut(s) 643
BslI CCNNNNNNNGG 4 cut(s) 81, 268, 667, 2165
BsmAI GTCTC 6 cut(s) 186, 203, 594, 688, 1164, 1428
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 643
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 5 cut(s) 117, 775, 1292, 1514, 1798
Bso31I GGTCTC 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 1659
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 151, 1239, 1830, 2140
Bsp1720I GCTNAGC 1 cut(s) 617
Bsp19I CCATGG 1 cut(s) 584
BspACI CCGC 3 cut(s) 470, 710, 1782
BspANI GGCC 5 cut(s) 117, 775, 1292, 1514, 1798
BspCNI CTCAG 4 cut(s) 479, 586, 609, 2095
BspHI TCATGA 2 cut(s) 1012, 1485
BspLI GGNNCC 2 cut(s) 1616, 1658
BspMI ACCTGC 1 cut(s) 1450
BspPI GGATC 2 cut(s) 146, 2135
BspQI GCTCTTC 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 594
BsrDI GCAATG 2 cut(s) 315, 2212
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 5 cut(s) 538, 1028, 1233, 1515, 1799
BssECI CCNNGG 6 cut(s) 584, 1293, 1637, 1660, 1980, 2200
BssMI GATC 4 cut(s) 151, 1239, 1830, 2140
BssNAI GTATAC 1 cut(s) 2059
BssT1I CCWWGG 3 cut(s) 584, 1293, 2200
Bst1107I GTATAC 1 cut(s) 2059
Bst2UI CCWGG 3 cut(s) 1211, 1661, 1982
Bst4CI ACNGT 6 cut(s) 104, 453, 565, 842, 1882, 1905
Bst6I CTCTTC 3 cut(s) 21, 1322, 2025
BstAPI GCANNNNNTGC 1 cut(s) 1451
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 655
BstC8I GCNNGC 3 cut(s) 93, 1336, 1477
BstDSI CCRYGG 1 cut(s) 584
BstF5I GGATG 7 cut(s) 273, 383, 542, 723, 1307, 1597, 2176
BstHHI GCGC 1 cut(s) 1957
BstKTI GATC 4 cut(s) 154, 1242, 1833, 2143
BstMAI GTCTC 6 cut(s) 186, 203, 594, 688, 1164, 1428
BstMBI GATC 4 cut(s) 151, 1239, 1830, 2140
BstMWI GCNNNNNNNGC 4 cut(s) 1191, 1451, 1520, 1705
BstNI CCWGG 3 cut(s) 1211, 1661, 1982
BstNSI RCATGY 1 cut(s) 477
BstSCI CCNGG 3 cut(s) 1209, 1659, 1980
BstSFI CTRYAG 1 cut(s) 798
BstV1I GCAGC 8 cut(s) 220, 223, 315, 687, 895, 1165, 1549, 1629
BstV2I GAAGAC 3 cut(s) 225, 250, 470
BstX2I RGATCY 1 cut(s) 2140
BstYI RGATCY 1 cut(s) 2140
BstZ17I GTATAC 1 cut(s) 2059
Bsu36I CCTNAGG 1 cut(s) 263
BsuI GTATCC 1 cut(s) 1053
BsuRI GGCC 5 cut(s) 117, 775, 1292, 1514, 1798
BtgI CCRYGG 1 cut(s) 584
BtsCI GGATG 7 cut(s) 273, 383, 542, 723, 1307, 1597, 2176
BtsI GCAGTG 1 cut(s) 1467
BtsIMutI CAGTG 6 cut(s) 449, 847, 1240, 1467, 1688, 1826
BveI ACCTGC 1 cut(s) 1450
Cac8I GCNNGC 3 cut(s) 93, 1336, 1477
CaiI CAGNNNCTG 3 cut(s) 697, 1397, 2144
CciI TCATGA 2 cut(s) 1012, 1485
CfoI GCGC 1 cut(s) 1957
Cfr13I GGNCC 2 cut(s) 794, 1512
CsiI ACCWGGT 1 cut(s) 1209
Csp6I GTAC 4 cut(s) 454, 1021, 1225, 1774
CviAII CATG 8 cut(s) 204, 474, 585, 777, 1013, 1099, 1486, 2225
CviQI GTAC 4 cut(s) 454, 1021, 1225, 1774
DpnI GATC 4 cut(s) 153, 1241, 1832, 2142
DpnII GATC 4 cut(s) 151, 1239, 1830, 2140
DriI GACNNNNNGTC 1 cut(s) 2233
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 21, 1322, 2025
Eam1105I GACNNNNNGTC 1 cut(s) 2233
EarI CTCTTC 3 cut(s) 21, 1322, 2025
Eco130I CCWWGG 3 cut(s) 584, 1293, 2200
Eco24I GRGCYC 1 cut(s) 1659
Eco31I GGTCTC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 794
Eco81I CCTNAGG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 794
EcoRII CCWGG 3 cut(s) 1209, 1659, 1980
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 3 cut(s) 584, 1293, 2200
EcoT22I ATGCAT 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 1659
ErhI CCWWGG 3 cut(s) 584, 1293, 2200
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 8 cut(s) 207, 477, 588, 780, 1016, 1102, 1489, 2228
FaqI GGGAC 1 cut(s) 643
FatI CATG 8 cut(s) 203, 473, 584, 776, 1012, 1098, 1485, 2224
FauNDI CATATG 1 cut(s) 1600
FblI GTMKAC 2 cut(s) 672, 2058
Fnu4HI GCNGC 9 cut(s) 234, 237, 329, 471, 701, 909, 1154, 1538, 1643
FokI GGATG 7 cut(s) 280, 370, 549, 730, 1314, 1604, 2183
FriOI GRGCYC 1 cut(s) 1659
Fsp4HI GCNGC 9 cut(s) 234, 237, 329, 471, 701, 909, 1154, 1538, 1643
FspBI CTAG 5 cut(s) 141, 213, 1148, 1425, 1455
GlaI GCGC 1 cut(s) 1956
GluI GCNGC 9 cut(s) 234, 237, 329, 471, 701, 909, 1154, 1538, 1643
HaeIII GGCC 5 cut(s) 117, 775, 1292, 1514, 1798
HhaI GCGC 1 cut(s) 1957
Hin1II CATG 8 cut(s) 207, 477, 588, 780, 1016, 1102, 1489, 2228
Hin6I GCGC 1 cut(s) 1955
HinP1I GCGC 1 cut(s) 1955
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HphI GGTGA 4 cut(s) 875, 1000, 1660, 1888
Hpy166II GTNNAC 7 cut(s) 396, 673, 761, 870, 1021, 1787, 2059
Hpy188I TCNGA 6 cut(s) 388, 693, 955, 1433, 1865, 2085
Hpy8I GTNNAC 7 cut(s) 396, 673, 761, 870, 1021, 1787, 2059
HpyAV CCTTC 3 cut(s) 114, 410, 1135
HpyCH4III ACNGT 6 cut(s) 104, 453, 565, 842, 1882, 1905
HpyCH4IV ACGT 6 cut(s) 145, 654, 738, 901, 1758, 1919
HpyF10VI GCNNNNNNNGC 4 cut(s) 1191, 1451, 1520, 1705
HpySE526I ACGT 6 cut(s) 145, 654, 738, 901, 1758, 1919
Hsp92II CATG 8 cut(s) 207, 477, 588, 780, 1016, 1102, 1489, 2228
HspAI GCGC 1 cut(s) 1955
Kzo9I GATC 4 cut(s) 151, 1239, 1830, 2140
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 856, 1662
Lsp1109I GCAGC 8 cut(s) 220, 223, 315, 687, 895, 1165, 1549, 1629
LweI GCATC 7 cut(s) 238, 693, 697, 928, 1194, 1347, 2161
MabI ACCWGGT 1 cut(s) 1209
MaeI CTAG 5 cut(s) 141, 213, 1148, 1425, 1455
MaeII ACGT 6 cut(s) 145, 654, 738, 901, 1758, 1919
MaeIII GTNAC 6 cut(s) 988, 1120, 1576, 1920, 2034, 2133
MalI GATC 4 cut(s) 153, 1241, 1832, 2142
MboI GATC 4 cut(s) 151, 1239, 1830, 2140
MfeI CAATTG 2 cut(s) 399, 754
MflI RGATCY 1 cut(s) 2140
MhlI GDGCHC 1 cut(s) 1659
MlyI GAGTC 5 cut(s) 389, 721, 922, 1135, 1604
Mph1103I ATGCAT 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 1370
MseI TTAA 7 cut(s) 36, 336, 411, 770, 924, 1868, 1907
MslI CAYNNNNRTG 2 cut(s) 1103, 1994
MspA1I CMGCKG 3 cut(s) 470, 908, 1156
MspR9I CCNGG 3 cut(s) 1211, 1661, 1982
MunI CAATTG 2 cut(s) 399, 754
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 3 cut(s) 1211, 1661, 1982
MwoI GCNNNNNNNGC 4 cut(s) 1191, 1451, 1520, 1705
NcoI CCATGG 1 cut(s) 584
NdeI CATATG 1 cut(s) 1600
NdeII GATC 4 cut(s) 151, 1239, 1830, 2140
NlaIII CATG 8 cut(s) 207, 477, 588, 780, 1016, 1102, 1489, 2228
NlaIV GGNNCC 2 cut(s) 1616, 1658
NmeAIII GCCGAG 1 cut(s) 1618
NmuCI GTSAC 1 cut(s) 988
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 1 cut(s) 477
PagI TCATGA 2 cut(s) 1012, 1485
PciSI GCTCTTC 1 cut(s) 21
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 1370
PfeI GAWTC 5 cut(s) 1030, 1246, 1558, 2196, 2210
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 9 cut(s) 235, 238, 330, 472, 702, 910, 1155, 1539, 1644
PleI GAGTC 5 cut(s) 388, 721, 922, 1135, 1603
PpsI GAGTC 5 cut(s) 388, 721, 922, 1135, 1603
Ppu21I YACGTR 1 cut(s) 655
PpuMI RGGWCCY 1 cut(s) 794
PshBI ATTAAT 1 cut(s) 36
PsiI TTATAA 2 cut(s) 461, 1205
Psp1406I AACGTT 1 cut(s) 901
Psp5II RGGWCCY 1 cut(s) 794
Psp6I CCWGG 3 cut(s) 1209, 1659, 1980
PspGI CCWGG 3 cut(s) 1209, 1659, 1980
PspN4I GGNNCC 2 cut(s) 1616, 1658
PspPI GGNCC 2 cut(s) 794, 1512
PspPPI RGGWCCY 1 cut(s) 794
PstNI CAGNNNCTG 3 cut(s) 697, 1397, 2144
PsuI RGATCY 1 cut(s) 2140
PvuII CAGCTG 2 cut(s) 908, 1156
RsaI GTAC 4 cut(s) 455, 1022, 1226, 1775
RsaNI GTAC 4 cut(s) 454, 1021, 1225, 1774
RseI CAYNNNNRTG 2 cut(s) 1103, 1994
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 7 cut(s) 36, 336, 411, 770, 924, 1868, 1907
SatI GCNGC 9 cut(s) 234, 237, 329, 471, 701, 909, 1154, 1538, 1643
Sau3AI GATC 4 cut(s) 151, 1239, 1830, 2140
Sau96I GGNCC 2 cut(s) 794, 1512
SchI GAGTC 5 cut(s) 389, 721, 922, 1135, 1604
ScrFI CCNGG 3 cut(s) 1211, 1661, 1982
SduI GDGCHC 1 cut(s) 1659
SexAI ACCWGGT 1 cut(s) 1209
SfaNI GCATC 7 cut(s) 238, 693, 697, 928, 1194, 1347, 2161
SfcI CTRYAG 1 cut(s) 798
SinI GGWCC 1 cut(s) 794
SmiMI CAYNNNNRTG 2 cut(s) 1103, 1994
SmlI CTYRAG 1 cut(s) 1189
SmoI CTYRAG 1 cut(s) 1189
SpeI ACTAGT 2 cut(s) 212, 1147
SsiI CCGC 3 cut(s) 470, 710, 1782
SspI AATATT 1 cut(s) 40
SspMI CTAG 5 cut(s) 141, 213, 1148, 1425, 1455
StyD4I CCNGG 3 cut(s) 1209, 1659, 1980
StyI CCWWGG 3 cut(s) 584, 1293, 2200
TaaI ACNGT 6 cut(s) 104, 453, 565, 842, 1882, 1905
TaiI ACGT 6 cut(s) 148, 657, 741, 904, 1761, 1922
TaqI TCGA 4 cut(s) 44, 827, 1048, 2093
TatI WGTACW 2 cut(s) 453, 1020
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 5 cut(s) 1030, 1246, 1558, 2196, 2210
Tru1I TTAA 7 cut(s) 36, 336, 411, 770, 924, 1868, 1907
Tru9I TTAA 7 cut(s) 36, 336, 411, 770, 924, 1868, 1907
TscAI CASTG 6 cut(s) 456, 847, 1240, 1467, 1695, 1833
TseFI GTSAC 1 cut(s) 988
TseI GCWGC 8 cut(s) 233, 236, 328, 700, 908, 1153, 1537, 1642
Tsp45I GTSAC 1 cut(s) 988
TspDTI ATGAA 8 cut(s) 428, 806, 1087, 1117, 1474, 1617, 2025, 2223
TspGWI ACGGA 3 cut(s) 563, 1078, 1327
TspRI CASTG 6 cut(s) 456, 847, 1240, 1467, 1695, 1833
Van91I CCANNNNNTGG 1 cut(s) 667
VpaK11BI GGWCC 1 cut(s) 794
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 623, 1632
XceI RCATGY 1 cut(s) 477
XmiI GTMKAC 2 cut(s) 672, 2058
XmnI GAANNNNTTC 1 cut(s) 1370
XspI CTAG 5 cut(s) 141, 213, 1148, 1425, 1455
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.