MD08G1076400.v1.1

CSC1-like protein RXW8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003874
Physical Location & Seq
Reverse (-)
6220211 .. 6226116
5906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1076400.v1.1.491

Sequence Viewer

Length: 2214 bp
ATGAATCTGGCAGCTCTTTTGACTTCTGCTGGTATTAATATAGCTGTGTGCGTGGTACTCTTTTTGCTATATTCTATATTGAGGAAACAACCGAGCAATGTGAATGTCTACTTTGGACGAATGCTTACGATATCCAAAAGAAGAAGTGATCCTTTCAATTTGGAAAGGTTTGTTCCTTCTGCTAGTTGGCTGGTGAAAGCATGGAGAACAAAAGAAGAAGATTTTTTGACTGTCGGTGGCTTGGATTCAGTTGTTTTTATCAGGATGCTTATTTTTAGTCTTCGAATCTTTTCCATTGCTGCTGTCATCTGCGTCTTTATAGTGCTTCCGGTGAATTATCATGGGCAAACGAAGGATCACCCGGGAATCCATGATGAATCCTTGGAAGTCTTTACCATCTTGAACGTTCAAGAAGGTTCAAAGTGGCTTTGGACCCATTGTCTCGCATTGTACATTATCACTACTTCAGCTTGTATTCTCCTATACTTTGAATATAATCACGTCACTAGACTGAGGCTGGCATATATTATTGGATCTTCTTCAAATCCAAGTCATTTTACAATTGTTGTTCGTGCTATTCCATGGTCACCGGAAGAATCATATAGTGATTCAGTAAGAAAATTCTTCACAAAATATCATGCATCAAGCTACTTGTCACACCAAATGGTGTATCGATCTGGTAAAGTTCAGAAACTGATGACCGATGCAGGGAAGATGTGCAAGATTCTTAAGGATGTTTCTGTGGAACAGAGTCATAGACCAGATCTATTTCACTGTGGTATGTCTGGAGGAGCTACTGATTCTTTTAAGATACTCGCGAACGATTCAGAGAGTGTTAAGGGGAAGTCATCCCTTATCGACAATAGTTTGGGTGGAAGAAAAAAGGTGTGTGCTGCTGCTTTTGTCTTTTTCAAGACTCGCTGTGCTGCTCTTGTTACATCAAAGGTTCTTCATTCTTCAAATCCCATGCATTGGGTGACAGATATGGCCCCGGAACCAAATGATGTGTATTGGTCAAACCTTTGGATACCATATGGACAACTGTGGATCCGGAAGTTAGCTACGCTTCTGGCTACTATTGCCTTTATGCTTGTGTTTCTTATTCCTGTCACATTTGTTCAGGGCATGACTGCACTAAAAAGCCTAGAAAAAAAGTTTCCAGCTTTGAAAGGATTGCTCAAAAAGAACTATGTAAGCCAGGTGATAACGGGGTACCTGCCAAGTGTGGTTTTAATTGTGGCTTTTTACATCGTTCCACCTGTCATGATGCTGTTTTCAACAGTGGAGGGGTGGATCTCTCGCAGTCAGAGGAAAAAGAGTGCATGCTTCAAAGTCTTATACTTCACAATCTGGAATGTCTTTTTTGTTAATGTTTTTACCGGCTCTGTTATCAGCCAATTGAGTGTATTTTCTAGTGTGAAAGACATACCTGCGCAACTTGCCAACGCGATTCCAGCACAGGCTAAGTTCTTCATGACTTATGTTTTATCATCTGGTTGGGCAAGCTTGGCATGTGAACTCATGCAAATTTATCCTCTTCTCTGCAACTATTTCCGACGGTTCATATTACGGAAAGGACGGTATAATGACTCACTGTCATTTCCATATCATACAGAAATTCCTAGAGTCCTGCTGTTTGGGTTCATCGGCTTCACCTGTTCTATCTTGGTTCCCCTAATATTGCCATTCTTGTTGGTTTACTTTGTACTTGCTTACTTTATATATCGAAACCAGATTCTTAATGTGTATATAGCAGAATACGAAAGTGGGGGACAGTACTGGCCTATGATTCACAATACAGTCATAATTTCATTGGTTATCATGCAAATAATTGCCCTCGGTGTCTTTGGATTAAGAAAATCACCAATTGCATCAGGTTTCACTATTCCACTGCTTATCTTCACTATTTTGTTTAACGAGTACTGTCGACAGCAGTTTCATCCATTGTTCAAGAACCAAGTTGCAGAGATTCTTATTGATATGGACCAGAAGGATGAGGAAGCGGGGAGGATGGAAGAGGTTTATCAACAGCTGCATACAGCTTACTGCCAGTCCCCATTAACTCCTGATGACTCGTGCAGTTTTGGATGCTCAACTCGCCATGTGGGCGCCATCATTCCGGATCCAGAGAATGTCAAGCCACCAGGGGAGGAACCCAGCCAAACTAATCCGTCATGGAATGTCACCTTCGACGGTGAGGCAGATCTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

738

Amino Acids

83.45

Weight (kDa)

8.99

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 164 1.2e-37 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 185 - 340 2.3e-25 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 351 - 616 1.7e-74 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1434
Acc36I ACCTGC 2 cut(s) 1224, 1438
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 2 cut(s) 1212, 2108
AccB7I CCANNNNNTGG 1 cut(s) 972
AccI GTMKAC 2 cut(s) 108, 1927
AccII CGCG 2 cut(s) 818, 1448
AccIII TCCGGA 2 cut(s) 1050, 2119
AciI CCGC 1 cut(s) 2003
AclI AACGTT 1 cut(s) 405
AclWI GGATC 8 cut(s) 143, 363, 541, 1042, 1055, 1301, 2117, 2130
AcsI RAATTY 3 cut(s) 620, 1527, 1617
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 2109
AfaI GTAC 6 cut(s) 57, 452, 1214, 1707, 1778, 1922
AfiI CCNNNNNNNGG 2 cut(s) 708, 972
AflII CTTAAG 1 cut(s) 728
AhdI GACNNNNNGTC 2 cut(s) 438, 1594
AjiI CACGTC 1 cut(s) 502
AjnI CCWGG 2 cut(s) 1197, 2143
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 8 cut(s) 143, 363, 541, 1042, 1055, 1301, 2117, 2130
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 361
Aor13HI TCCGGA 2 cut(s) 1050, 2119
AoxI GGCC 2 cut(s) 987, 1781
ApeKI GCWGC 6 cut(s) 11, 299, 893, 896, 926, 2032
ApoI RAATTY 3 cut(s) 620, 1527, 1617
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 289
Asp718I GGTACC 1 cut(s) 1212
AspLEI GCGC 2 cut(s) 1435, 2111
AspS9I GGNCC 3 cut(s) 432, 988, 1984
AsuC2I CCSGG 3 cut(s) 362, 363, 992
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 2 cut(s) 432, 1984
BamHI GGATCC 2 cut(s) 1047, 2122
BanI GGYRCC 2 cut(s) 1212, 2108
BauI CACGAG 1 cut(s) 2074
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 6 cut(s) 23, 286, 880, 883, 913, 2019
BccI CCATC 3 cut(s) 404, 2005, 2120
BciT130I CCWGG 2 cut(s) 1199, 2145
BciVI GTATCC 1 cut(s) 1020
BcnI CCSGG 3 cut(s) 362, 363, 992
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 5 cut(s) 183, 507, 1145, 1413, 1623
BfoI RGCGCY 1 cut(s) 2112
BfrI CTTAAG 1 cut(s) 728
BfuAI ACCTGC 2 cut(s) 1224, 1438
BfuI GTATCC 1 cut(s) 1020
BglI GCCNNNNNGGC 1 cut(s) 2106
BglII AGATCT 2 cut(s) 763, 2203
BisI GCNGC 6 cut(s) 12, 300, 894, 897, 927, 2033
BlsI GCNGC 6 cut(s) 13, 301, 895, 898, 928, 2034
BmcAI AGTACT 2 cut(s) 1778, 1922
Bme1390I CCNGG 5 cut(s) 362, 363, 992, 1199, 2145
Bme18I GGWCC 2 cut(s) 432, 1984
BmeRI GACNNNNNGTC 2 cut(s) 438, 1594
BmeT110I CYCGRG 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 432, 988, 1984
BmiI GGNNCC 9 cut(s) 434, 990, 996, 1049, 1214, 1671, 2110, 2124, 2154
BmrFI CCNGG 5 cut(s) 362, 363, 992, 1199, 2145
BmsI GCATC 6 cut(s) 255, 650, 694, 1257, 1880, 2078
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 283
BpuMI CCSGG 3 cut(s) 362, 363, 992
Bsa29I ATCGAT 1 cut(s) 673
BsaHI GRCGYC 1 cut(s) 2109
BsaJI CCNNGG 6 cut(s) 361, 381, 581, 990, 1837, 2144
BsaWI WCCGGW 4 cut(s) 328, 589, 1050, 2119
Bsc4I CCNNNNNNNGG 2 cut(s) 708, 972
Bse118I RCCGGY 1 cut(s) 1379
Bse1I ACTGG 2 cut(s) 1784, 2050
Bse3DI GCAATG 2 cut(s) 103, 294
BseAI TCCGGA 2 cut(s) 1050, 2119
BseBI CCWGG 2 cut(s) 1199, 2145
BseCI ATCGAT 1 cut(s) 673
BseDI CCNNGG 6 cut(s) 361, 381, 581, 990, 1837, 2144
BseGI GGATG 7 cut(s) 270, 739, 848, 1939, 1999, 2016, 2093
BseLI CCNNNNNNNGG 2 cut(s) 708, 972
BseMI GCAATG 2 cut(s) 103, 294
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 2 cut(s) 1784, 2050
BseRI GAGGAG 1 cut(s) 804
BseXI GCAGC 6 cut(s) 23, 286, 880, 883, 913, 2019
BseYI CCCAGC 1 cut(s) 2156
BsgI GTGCAG 2 cut(s) 1116, 2098
Bsh1236I CGCG 2 cut(s) 818, 1448
BshFI GGCC 2 cut(s) 989, 1783
BshNI GGYRCC 2 cut(s) 1212, 2108
BshVI ATCGAT 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 7 cut(s) 329, 362, 590, 992, 1051, 1380, 2120
BslFI GGGAC 2 cut(s) 1785, 2038
BslI CCNNNNNNNGG 2 cut(s) 708, 972
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 2 cut(s) 1785, 2038
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 2 cut(s) 989, 1783
BsoBI CYCGRG 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 283
Bsp13I TCCGGA 2 cut(s) 1050, 2119
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
Bsp19I CCATGG 1 cut(s) 581
Bsp68I TCGCGA 1 cut(s) 818
BspACI CCGC 1 cut(s) 2003
BspANI GGCC 2 cut(s) 989, 1783
BspCNI CTCAG 1 cut(s) 504
BspDI ATCGAT 1 cut(s) 673
BspEI TCCGGA 2 cut(s) 1050, 2119
BspFNI CGCG 2 cut(s) 818, 1448
BspHI TCATGA 2 cut(s) 1263, 1473
BspLI GGNNCC 9 cut(s) 434, 990, 996, 1049, 1214, 1671, 2110, 2124, 2154
BspMI ACCTGC 2 cut(s) 1224, 1438
BspPI GGATC 8 cut(s) 143, 363, 541, 1042, 1055, 1301, 2117, 2130
BspT104I TTCGAA 1 cut(s) 283
BspT107I GGYRCC 2 cut(s) 1212, 2108
BspTI CTTAAG 1 cut(s) 728
BsrDI GCAATG 2 cut(s) 103, 294
BsrFI RCCGGY 1 cut(s) 1379
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 2 cut(s) 1784, 2050
BssAI RCCGGY 1 cut(s) 1379
BssECI CCNNGG 6 cut(s) 361, 381, 581, 990, 1837, 2144
BssMI GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
BssNI GRCGYC 1 cut(s) 2109
BssSI CACGAG 1 cut(s) 2074
BssT1I CCWWGG 2 cut(s) 381, 581
Bst2BI CACGAG 1 cut(s) 2074
Bst2UI CCWGG 2 cut(s) 1199, 2145
Bst6I CTCTTC 2 cut(s) 1542, 2010
BstACI GRCGYC 1 cut(s) 2109
BstAFI CTTAAG 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 3 cut(s) 519, 1324, 1504
BstDEI CTNAG 2 cut(s) 512, 1464
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 585
BstF5I GGATG 7 cut(s) 270, 739, 848, 1939, 1999, 2016, 2093
BstFNI CGCG 2 cut(s) 818, 1448
BstH2I RGCGCY 1 cut(s) 2112
BstHHI GCGC 2 cut(s) 1435, 2111
BstKTI GATC 9 cut(s) 151, 358, 536, 677, 766, 1050, 1296, 2125, 2206
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
BstMWI GCNNNNNNNGC 6 cut(s) 1079, 1439, 1454, 1508, 2097, 2106
BstNI CCWGG 2 cut(s) 1199, 2145
BstNSI RCATGY 2 cut(s) 1326, 1515
BstPI GGTNACC 1 cut(s) 585
BstSCI CCNGG 5 cut(s) 360, 361, 990, 1197, 2143
BstUI CGCG 2 cut(s) 818, 1448
BstV1I GCAGC 6 cut(s) 23, 286, 880, 883, 913, 2019
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 6 cut(s) 533, 763, 1047, 1293, 2122, 2203
BstYI RGATCY 6 cut(s) 533, 763, 1047, 1293, 2122, 2203
Bsu15I ATCGAT 1 cut(s) 673
BsuI GTATCC 1 cut(s) 1020
BsuRI GGCC 2 cut(s) 989, 1783
BsuTUI ATCGAT 1 cut(s) 673
BtgI CCRYGG 1 cut(s) 581
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 7 cut(s) 270, 739, 848, 1939, 1999, 2016, 2093
BtsI GCAGTG 1 cut(s) 1889
BtsIMutI CAGTG 4 cut(s) 772, 1287, 1592, 1889
BtuMI TCGCGA 1 cut(s) 818
BveI ACCTGC 2 cut(s) 1224, 1438
Cac8I GCNNGC 3 cut(s) 519, 1324, 1504
CaiI CAGNNNCTG 1 cut(s) 694
CciI TCATGA 2 cut(s) 1263, 1473
CfoI GCGC 2 cut(s) 1435, 2111
Cfr10I RCCGGY 1 cut(s) 1379
Cfr13I GGNCC 3 cut(s) 432, 988, 1984
Cfr9I CCCGGG 1 cut(s) 361
ClaI ATCGAT 1 cut(s) 673
CseI GACGC 1 cut(s) 301
Csp6I GTAC 6 cut(s) 56, 451, 1213, 1706, 1777, 1921
CviQI GTAC 6 cut(s) 56, 451, 1213, 1706, 1777, 1921
DdeI CTNAG 2 cut(s) 512, 1464
DinI GGCGCC 1 cut(s) 2110
DpnI GATC 9 cut(s) 150, 357, 535, 676, 765, 1049, 1295, 2124, 2205
DpnII GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
DriI GACNNNNNGTC 2 cut(s) 438, 1594
Eam1104I CTCTTC 2 cut(s) 1542, 2010
Eam1105I GACNNNNNGTC 2 cut(s) 438, 1594
EarI CTCTTC 2 cut(s) 1542, 2010
Eco130I CCWWGG 2 cut(s) 381, 581
Eco32I GATATC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 432, 1984
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 361
Eco91I GGTNACC 1 cut(s) 585
EcoO65I GGTNACC 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 1197, 2143
EcoRV GATATC 1 cut(s) 132
EcoT14I CCWWGG 2 cut(s) 381, 581
EcoT22I ATGCAT 2 cut(s) 643, 972
EgeI GGCGCC 1 cut(s) 2110
EheI GGCGCC 1 cut(s) 2110
ErhI CCWWGG 2 cut(s) 381, 581
FalI AAGNNNNNCTT 2 cut(s) 136, 168
FaqI GGGAC 2 cut(s) 1785, 2038
FauI CCCGC 1 cut(s) 1996
FauNDI CATATG 1 cut(s) 1033
FblI GTMKAC 2 cut(s) 108, 1927
Fnu4HI GCNGC 6 cut(s) 12, 300, 894, 897, 927, 2033
FokI GGATG 7 cut(s) 277, 746, 835, 1926, 2006, 2023, 2100
Fsp4HI GCNGC 6 cut(s) 12, 300, 894, 897, 927, 2033
FspBI CTAG 5 cut(s) 183, 507, 1145, 1413, 1623
FspI TGCGCA 1 cut(s) 1434
GlaI GCGC 2 cut(s) 1434, 2110
GluI GCNGC 6 cut(s) 12, 300, 894, 897, 927, 2033
GsaI CCCAGC 1 cut(s) 2160
GsuI CTGGAG 1 cut(s) 807
HaeII RGCGCY 1 cut(s) 2112
HaeIII GGCC 2 cut(s) 989, 1783
HapII CCGG 7 cut(s) 329, 362, 590, 992, 1051, 1380, 2120
HgaI GACGC 1 cut(s) 301
HhaI GCGC 2 cut(s) 1435, 2111
Hin1I GRCGYC 1 cut(s) 2109
Hin6I GCGC 2 cut(s) 1433, 2109
HinP1I GCGC 2 cut(s) 1433, 2109
HincII GTYRAC 1 cut(s) 1928
HindII GTYRAC 1 cut(s) 1928
HindIII AAGCTT 1 cut(s) 1504
HpaII CCGG 7 cut(s) 329, 362, 590, 992, 1051, 1380, 2120
Hpy166II GTNNAC 4 cut(s) 109, 1517, 1699, 1928
Hpy188I TCNGA 4 cut(s) 690, 829, 1308, 1556
Hpy8I GTNNAC 4 cut(s) 109, 1517, 1699, 1928
Hpy99I CGWCG 2 cut(s) 1560, 2195
HpyAV CCTTC 5 cut(s) 186, 346, 407, 1984, 2197
HpyCH4IV ACGT 2 cut(s) 405, 501
HpyF10VI GCNNNNNNNGC 6 cut(s) 1079, 1439, 1454, 1508, 2097, 2106
HpyF3I CTNAG 2 cut(s) 512, 1464
HpySE526I ACGT 2 cut(s) 405, 501
Hsp92I GRCGYC 1 cut(s) 2109
HspAI GCGC 2 cut(s) 1433, 2109
KasI GGCGCC 1 cut(s) 2108
Kpn2I TCCGGA 2 cut(s) 1050, 2119
KpnI GGTACC 1 cut(s) 1216
Kzo9I GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
LmnI GCTCC 1 cut(s) 791
Lsp1109I GCAGC 6 cut(s) 23, 286, 880, 883, 913, 2019
LweI GCATC 6 cut(s) 255, 650, 694, 1257, 1880, 2078
MaeI CTAG 5 cut(s) 183, 507, 1145, 1413, 1623
MaeII ACGT 2 cut(s) 405, 501
MaeIII GTNAC 7 cut(s) 502, 585, 654, 934, 976, 1108, 2182
MalI GATC 9 cut(s) 150, 357, 535, 676, 765, 1049, 1295, 2124, 2205
MboI GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
MfeI CAATTG 3 cut(s) 561, 1397, 1866
MflI RGATCY 6 cut(s) 533, 763, 1047, 1293, 2122, 2203
Mly113I GGCGCC 1 cut(s) 2109
MlyI GAGTC 5 cut(s) 760, 910, 1583, 1635, 2066
MmeI TCCRAC 1 cut(s) 1579
Mph1103I ATGCAT 2 cut(s) 643, 972
MroI TCCGGA 2 cut(s) 1050, 2119
MroXI GAANNNNTTC 1 cut(s) 289
MspA1I CMGCKG 1 cut(s) 2032
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 7 cut(s) 329, 362, 590, 992, 1051, 1380, 2120
MspR9I CCNGG 5 cut(s) 362, 363, 992, 1199, 2145
MunI CAATTG 3 cut(s) 561, 1397, 1866
Mva1269I GAATGC 1 cut(s) 126
MvaI CCWGG 2 cut(s) 1199, 2145
MvnI CGCG 2 cut(s) 818, 1448
MwoI GCNNNNNNNGC 6 cut(s) 1079, 1439, 1454, 1508, 2097, 2106
NarI GGCGCC 1 cut(s) 2109
NciI CCSGG 3 cut(s) 362, 363, 992
NcoI CCATGG 1 cut(s) 581
NdeI CATATG 1 cut(s) 1033
NdeII GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
NlaIV GGNNCC 9 cut(s) 434, 990, 996, 1049, 1214, 1671, 2110, 2124, 2154
NmuCI GTSAC 6 cut(s) 502, 585, 654, 976, 1108, 2182
NruI TCGCGA 1 cut(s) 818
NsbI TGCGCA 1 cut(s) 1434
NsiI ATGCAT 2 cut(s) 643, 972
NspI RCATGY 2 cut(s) 1326, 1515
NspV TTCGAA 1 cut(s) 283
PaeI GCATGC 1 cut(s) 1326
PagI TCATGA 2 cut(s) 1263, 1473
PctI GAATGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 972
PkrI GCNGC 6 cut(s) 13, 301, 895, 898, 928, 2034
PleI GAGTC 5 cut(s) 759, 910, 1583, 1634, 2066
PluTI GGCGCC 1 cut(s) 2112
PpsI GAGTC 5 cut(s) 759, 910, 1583, 1634, 2066
PshBI ATTAAT 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 1197, 2143
PspEI GGTNACC 1 cut(s) 585
PspFI CCCAGC 1 cut(s) 2156
PspGI CCWGG 2 cut(s) 1197, 2143
PspN4I GGNNCC 9 cut(s) 434, 990, 996, 1049, 1214, 1671, 2110, 2124, 2154
PspPI GGNCC 3 cut(s) 432, 988, 1984
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 6 cut(s) 533, 763, 1047, 1293, 2122, 2203
PvuII CAGCTG 1 cut(s) 2032
RruI TCGCGA 1 cut(s) 818
RsaI GTAC 6 cut(s) 57, 452, 1214, 1707, 1778, 1922
RsaNI GTAC 6 cut(s) 56, 451, 1213, 1706, 1777, 1921
SalI GTCGAC 1 cut(s) 1926
SatI GCNGC 6 cut(s) 12, 300, 894, 897, 927, 2033
Sau3AI GATC 9 cut(s) 148, 355, 533, 674, 763, 1047, 1293, 2122, 2203
Sau96I GGNCC 3 cut(s) 432, 988, 1984
ScaI AGTACT 2 cut(s) 1778, 1922
SchI GAGTC 5 cut(s) 760, 910, 1583, 1635, 2066
ScrFI CCNGG 5 cut(s) 362, 363, 992, 1199, 2145
SfaNI GCATC 6 cut(s) 255, 650, 694, 1257, 1880, 2078
SfoI GGCGCC 1 cut(s) 2110
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 2 cut(s) 432, 1984
SmaI CCCGGG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SphI GCATGC 1 cut(s) 1326
SsiI CCGC 1 cut(s) 2003
SspDI GGCGCC 1 cut(s) 2108
SspI AATATT 1 cut(s) 1680
SspMI CTAG 5 cut(s) 183, 507, 1145, 1413, 1623
StyD4I CCNGG 5 cut(s) 360, 361, 990, 1197, 2143
StyI CCWWGG 2 cut(s) 381, 581
TaiI ACGT 2 cut(s) 408, 504
TaqI TCGA 6 cut(s) 283, 673, 858, 1726, 1927, 2190
TaqII GACCGA 1 cut(s) 716
TatI WGTACW 4 cut(s) 450, 1705, 1776, 1920
TscAI CASTG 4 cut(s) 779, 1287, 1599, 1896
TseFI GTSAC 6 cut(s) 502, 585, 654, 976, 1108, 2182
TseI GCWGC 6 cut(s) 11, 299, 893, 896, 926, 2032
Tsp45I GTSAC 6 cut(s) 502, 585, 654, 976, 1108, 2182
TspDTI ATGAA 8 cut(s) 17, 390, 941, 1462, 1552, 1633, 1800, 1928
TspGWI ACGGA 2 cut(s) 1585, 2160
TspMI CCCGGG 1 cut(s) 361
TspRI CASTG 4 cut(s) 779, 1287, 1599, 1896
Van91I CCANNNNNTGG 1 cut(s) 972
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 2 cut(s) 432, 1984
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 3 cut(s) 620, 1527, 1617
XceI RCATGY 2 cut(s) 1326, 1515
XmaI CCCGGG 1 cut(s) 361
XmiI GTMKAC 2 cut(s) 108, 1927
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 5 cut(s) 183, 507, 1145, 1413, 1623
ZrmI AGTACT 2 cut(s) 1778, 1922
Zsp2I ATGCAT 2 cut(s) 643, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.