MD15G1063600.v1.1

CSC1-like protein RXW8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003874
Physical Location & Seq
Reverse (-)
4374181 .. 4378933
4753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1063600.v1.1.491

Sequence Viewer

Length: 1785 bp
ATGAATCTTGCAGCTCTTTTGACTTCTGCGGGGATTAATATAGCTGTGTGCGTGATACTCTTTTCGCTATATTCTGTATTGAGGAAACAACCGTGCAATGTGAATGTCTACTTTGGCCGAACGGTTTCGATAACCAAAAGAAGAAGTGATCCTTTCTGCTTGGAAAGGTTTGTACCTTCTGCTAGTTGGCTAGTGAAAGCATGGAGAACAAGAGAAGATGATTTATTGGATGTTGGTGGCTTGGATTCAGTAGTTTTTATCAGGATGGTTATTTTTAGTCTTCGAATCTTTTCCATTGCTGCTGTCATATGCGTCTTTATAGTACTTCCGGTGAATTATCATGGGCAAACCACGAATCACCCGGGAATCCCTGAAGAATCCTTGGAAGTCTTTACCATCTTGAATGTTCAAGAAGGTTCAAAGTGGCTCTGGACCCATTGTCTCGCATTGTACATTATCACTGCTTCAGCTTGTATTCTCCTATACTTTGAATATAAGCACATCACTAGACTGAGGCTGGCATATATTACTGGATCTTCTTTAAGTCCAAGTCATTTTACACTTGTTGTTCGTGCTATCCCATGGTCACCGGAAGAATCATATAGTGATTCAGTAAGAAAATTCTTCACAAAATATCACGCATCAAGCTACTTGTCACACCAAATGGTGTATCGAGTTGGTAAAGTTCAGAAACTGATGAGCAATGCAGGGAGGATGTGCAAAATTCTTAGGGATGTTTCTGTGGAACAGACTCATACACCAGATTTATTTCATTGTGGTATGTGTGGAGGAGCTACTGATTCTTTAAAGATACTTGCGACCGATTCAGAGAGTGTTAAAGGGAAGTCATCCCTCATCGACAATAGTTTGGGTGGTAGAAAAAAGGTGTGTGCTGCTGCTTTTGTCTTTTTCAAAACTCGCTATGCTGCTGTTGTTACTTCAAAGGTTCTTCAGTCTTCAAATCCCATGCATTGGGTGACAGATATGGCTCCGGAACCAAAGGATGTTTATTGGTCAAACCTTTGGATACCGTACGGACAACTGTGGATCCGGAAGATAGCTACACTTCTGGCTACTATTGCTTTTATGCTTGTGTTTCTTATTCCTGTCACATTTGTTCAGGGCATGACTGCACTAAAAAGTCTAGAAAAGAAATTTCCAGCTTTGAAAGGATTACTCAAAAAGAAATTTATGAGCCAGGTGATAACGGGGTACCTACCAAGTGTGGTTTTAATTCTGGCTTTTTACGTAGTTCCACCAGTCATGATGCTGTTTTCAACAGTGGAGGGGTGGATCTCCCGCAGTCAGAGGAAAAAGAGTGCATGCTTCAAAGTCTTGTACTTCACAATCTGGAATGTCTTTTTTGTTAATGTTTTTACCGGTTCTGTTATCAGCCAGTTGAGTGTATTTTCTAGTGTGAAAGACATACCTGGGCAACTTGCCAATGCAGTTCCAGCACAGGCTAAGTTCTTCATGACTTATGTTTTATCATCTGGTTGGGCAAGCTTGGCATGTGAACTCATGCAAATTTATCCTCTTCTTTGCAACTATATCCGACGGTTCATATTACTGAAAGAAGGGTATAATGACACACTGTCATTTCCATATCATACAGAAATTCCTAGAGTCCTGCTGTTTGGGTTCATCGGCTTCACCTGTTCTATCTTGGTTCCCCTAATATTGCCATTCTTGCTGGTTTACTTTGTACTTGCTTACTTTATATATCGAAACCAGGTGAGGACTTCTCCTATCTATCATTCCTTTTTCTGTCAGGTCTCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

595

Amino Acids

67.26

Weight (kDa)

9.49

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 164 1e-36 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 185 - 340 2.6e-27 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 351 - 579 1.9e-59 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 1 cut(s) 1212
AccB7I CCANNNNNTGG 1 cut(s) 972
AccI GTMKAC 1 cut(s) 108
AccIII TCCGGA 2 cut(s) 991, 1050
AciI CCGC 2 cut(s) 29, 1300
AclWI GGATC 5 cut(s) 143, 541, 1042, 1055, 1301
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 6 cut(s) 620, 723, 1154, 1187, 1527, 1617
AcuI CTGAAG 3 cut(s) 393, 450, 935
AfaI GTAC 7 cut(s) 174, 324, 452, 1034, 1214, 1340, 1707
AfiI CCNNNNNNNGG 1 cut(s) 972
AgeI ACCGGT 1 cut(s) 1379
AhdI GACNNNNNGTC 2 cut(s) 438, 1594
AjnI CCWGG 3 cut(s) 1197, 1429, 1731
AluBI AGCT 8 cut(s) 14, 44, 470, 648, 794, 1061, 1163, 1506
AluI AGCT 8 cut(s) 14, 44, 470, 648, 794, 1061, 1163, 1506
Alw26I GTCTC 2 cut(s) 446, 1780
AlwI GGATC 5 cut(s) 143, 541, 1042, 1055, 1301
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 361
Aor13HI TCCGGA 2 cut(s) 991, 1050
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 5 cut(s) 11, 299, 893, 896, 926
ApoI RAATTY 6 cut(s) 620, 723, 1154, 1187, 1527, 1617
AseI ATTAAT 1 cut(s) 36
AsiGI ACCGGT 1 cut(s) 1379
Asp700I GAANNNNTTC 2 cut(s) 124, 289
Asp718I GGTACC 1 cut(s) 1212
AspS9I GGNCC 1 cut(s) 432
AsuC2I CCSGG 2 cut(s) 362, 363
AsuHPI GGTGA 7 cut(s) 343, 350, 579, 988, 1213, 1645, 1747
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 1047
BanI GGYRCC 1 cut(s) 1212
BbsI GAAGAC 2 cut(s) 272, 948
BbvI GCAGC 5 cut(s) 23, 286, 880, 883, 913
BccI CCATC 2 cut(s) 259, 404
BciT130I CCWGG 3 cut(s) 1199, 1431, 1733
BciVI GTATCC 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 362, 363
BcoDI GTCTC 2 cut(s) 446, 1780
BfaI CTAG 6 cut(s) 183, 191, 507, 1145, 1413, 1623
BfuI GTATCC 1 cut(s) 1020
BisI GCNGC 5 cut(s) 12, 300, 894, 897, 927
BlsI GCNGC 5 cut(s) 13, 301, 895, 898, 928
BmcAI AGTACT 1 cut(s) 324
Bme1390I CCNGG 5 cut(s) 362, 363, 1199, 1431, 1733
Bme18I GGWCC 1 cut(s) 432
BmeRI GACNNNNNGTC 2 cut(s) 438, 1594
BmeT110I CYCGRG 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 6 cut(s) 434, 990, 996, 1049, 1214, 1671
BmrFI CCNGG 5 cut(s) 362, 363, 1199, 1431, 1733
BmsI GCATC 2 cut(s) 650, 1257
BpiI GAAGAC 2 cut(s) 272, 948
BplI GAGNNNNNCTC 2 cut(s) 1729, 1761
Bpu14I TTCGAA 1 cut(s) 283
BpuMI CCSGG 2 cut(s) 362, 363
BsaAI YACGTR 1 cut(s) 1249
BsaI GGTCTC 1 cut(s) 1780
BsaJI CCNNGG 4 cut(s) 361, 381, 581, 1430
BsaWI WCCGGW 5 cut(s) 328, 589, 991, 1050, 1379
Bsc4I CCNNNNNNNGG 1 cut(s) 972
Bse118I RCCGGY 1 cut(s) 1379
Bse1I ACTGG 3 cut(s) 535, 1259, 1396
Bse3DI GCAATG 3 cut(s) 103, 294, 709
BseAI TCCGGA 2 cut(s) 991, 1050
BseBI CCWGG 3 cut(s) 1199, 1431, 1733
BseDI CCNNGG 4 cut(s) 361, 381, 581, 1430
BseGI GGATG 6 cut(s) 235, 270, 720, 739, 848, 1009
BseLI CCNNNNNNNGG 1 cut(s) 972
BseMI GCAATG 3 cut(s) 103, 294, 709
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 3 cut(s) 535, 1259, 1396
BseRI GAGGAG 1 cut(s) 804
BseXI GCAGC 5 cut(s) 23, 286, 880, 883, 913
BsgI GTGCAG 1 cut(s) 1116
Bsh1285I CGRYCG 1 cut(s) 822
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 1212
BshTI ACCGGT 1 cut(s) 1379
BsiEI CGRYCG 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 6 cut(s) 329, 362, 590, 992, 1051, 1380
BsiWI CGTACG 1 cut(s) 1032
BslI CCNNNNNNNGG 1 cut(s) 972
BsmAI GTCTC 2 cut(s) 446, 1780
BsnI GGCC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 1780
BsoBI CYCGRG 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 283
Bsp13I TCCGGA 2 cut(s) 991, 1050
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 4 cut(s) 148, 533, 1047, 1293
Bsp19I CCATGG 1 cut(s) 581
BspACI CCGC 2 cut(s) 29, 1300
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 504
BspEI TCCGGA 2 cut(s) 991, 1050
BspHI TCATGA 2 cut(s) 1263, 1473
BspLI GGNNCC 6 cut(s) 434, 990, 996, 1049, 1214, 1671
BspPI GGATC 5 cut(s) 143, 541, 1042, 1055, 1301
BspT104I TTCGAA 1 cut(s) 283
BspT107I GGYRCC 1 cut(s) 1212
BspTNI GGTCTC 1 cut(s) 1780
BsrDI GCAATG 3 cut(s) 103, 294, 709
BsrFI RCCGGY 1 cut(s) 1379
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 3 cut(s) 535, 1259, 1396
BssAI RCCGGY 1 cut(s) 1379
BssECI CCNNGG 4 cut(s) 361, 381, 581, 1430
BssMI GATC 4 cut(s) 148, 533, 1047, 1293
BssT1I CCWWGG 2 cut(s) 381, 581
Bst2UI CCWGG 3 cut(s) 1199, 1431, 1733
Bst4CI ACNGT 7 cut(s) 93, 124, 1032, 1044, 1282, 1560, 1596
Bst6I CTCTTC 1 cut(s) 1542
BstAUI TGTACA 1 cut(s) 450
BstBAI YACGTR 1 cut(s) 1249
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 3 cut(s) 519, 1324, 1504
BstDEI CTNAG 3 cut(s) 512, 728, 1464
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 585
BstF5I GGATG 6 cut(s) 235, 270, 720, 739, 848, 1009
BstKTI GATC 4 cut(s) 151, 536, 1050, 1296
BstMAI GTCTC 2 cut(s) 446, 1780
BstMBI GATC 4 cut(s) 148, 533, 1047, 1293
BstMCI CGRYCG 1 cut(s) 822
BstMWI GCNNNNNNNGC 4 cut(s) 1079, 1454, 1508, 1690
BstNI CCWGG 3 cut(s) 1199, 1431, 1733
BstNSI RCATGY 2 cut(s) 1326, 1515
BstPI GGTNACC 1 cut(s) 585
BstSCI CCNGG 5 cut(s) 360, 361, 1197, 1429, 1731
BstSNI TACGTA 1 cut(s) 1249
BstV1I GCAGC 5 cut(s) 23, 286, 880, 883, 913
BstV2I GAAGAC 2 cut(s) 272, 948
BstX2I RGATCY 3 cut(s) 533, 1047, 1293
BstYI RGATCY 3 cut(s) 533, 1047, 1293
BsuI GTATCC 1 cut(s) 1020
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 581
BtsCI GGATG 6 cut(s) 235, 270, 720, 739, 848, 1009
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 459, 1287, 1592
Cac8I GCNNGC 3 cut(s) 519, 1324, 1504
CaiI CAGNNNCTG 1 cut(s) 694
CciI TCATGA 2 cut(s) 1263, 1473
Cfr10I RCCGGY 1 cut(s) 1379
Cfr13I GGNCC 1 cut(s) 432
Cfr9I CCCGGG 1 cut(s) 361
CseI GACGC 1 cut(s) 301
CsiI ACCWGGT 1 cut(s) 1731
Csp6I GTAC 7 cut(s) 173, 323, 451, 1033, 1213, 1339, 1706
CspAI ACCGGT 1 cut(s) 1379
CviQI GTAC 7 cut(s) 173, 323, 451, 1033, 1213, 1339, 1706
DdeI CTNAG 3 cut(s) 512, 728, 1464
DpnI GATC 4 cut(s) 150, 535, 1049, 1295
DpnII GATC 4 cut(s) 148, 533, 1047, 1293
DraI TTTAAA 1 cut(s) 807
DriI GACNNNNNGTC 2 cut(s) 438, 1594
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 1542
Eam1105I GACNNNNNGTC 2 cut(s) 438, 1594
EarI CTCTTC 1 cut(s) 1542
Eco105I TACGTA 1 cut(s) 1249
Eco130I CCWWGG 2 cut(s) 381, 581
Eco31I GGTCTC 1 cut(s) 1780
Eco47I GGWCC 1 cut(s) 432
Eco57I CTGAAG 3 cut(s) 393, 450, 935
Eco88I CYCGRG 1 cut(s) 361
Eco91I GGTNACC 1 cut(s) 585
EcoO65I GGTNACC 1 cut(s) 585
EcoRII CCWGG 3 cut(s) 1197, 1429, 1731
EcoT14I CCWWGG 2 cut(s) 381, 581
EcoT22I ATGCAT 1 cut(s) 972
ErhI CCWWGG 2 cut(s) 381, 581
FalI AAGNNNNNCTT 2 cut(s) 136, 168
FauI CCCGC 2 cut(s) 22, 1307
FauNDI CATATG 1 cut(s) 308
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 5 cut(s) 12, 300, 894, 897, 927
FokI GGATG 6 cut(s) 242, 277, 727, 746, 835, 1016
Fsp4HI GCNGC 5 cut(s) 12, 300, 894, 897, 927
FspBI CTAG 6 cut(s) 183, 191, 507, 1145, 1413, 1623
GluI GCNGC 5 cut(s) 12, 300, 894, 897, 927
HaeIII GGCC 1 cut(s) 117
HapII CCGG 6 cut(s) 329, 362, 590, 992, 1051, 1380
HgaI GACGC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 1504
HpaII CCGG 6 cut(s) 329, 362, 590, 992, 1051, 1380
HphI GGTGA 7 cut(s) 343, 350, 579, 988, 1213, 1645, 1747
Hpy166II GTNNAC 3 cut(s) 109, 1517, 1699
Hpy188I TCNGA 4 cut(s) 690, 829, 1308, 1556
Hpy8I GTNNAC 3 cut(s) 109, 1517, 1699
Hpy99I CGWCG 1 cut(s) 1560
HpyAV CCTTC 3 cut(s) 186, 407, 1571
HpyCH4III ACNGT 7 cut(s) 93, 124, 1032, 1044, 1282, 1560, 1596
HpyCH4IV ACGT 1 cut(s) 1248
HpyF10VI GCNNNNNNNGC 4 cut(s) 1079, 1454, 1508, 1690
HpyF3I CTNAG 3 cut(s) 512, 728, 1464
HpySE526I ACGT 1 cut(s) 1248
Kpn2I TCCGGA 2 cut(s) 991, 1050
KpnI GGTACC 1 cut(s) 1216
Kzo9I GATC 4 cut(s) 148, 533, 1047, 1293
LmnI GCTCC 2 cut(s) 791, 994
Lsp1109I GCAGC 5 cut(s) 23, 286, 880, 883, 913
LweI GCATC 2 cut(s) 650, 1257
MabI ACCWGGT 1 cut(s) 1731
MaeI CTAG 6 cut(s) 183, 191, 507, 1145, 1413, 1623
MaeII ACGT 1 cut(s) 1248
MaeIII GTNAC 5 cut(s) 585, 654, 934, 976, 1108
MalI GATC 4 cut(s) 150, 535, 1049, 1295
MboI GATC 4 cut(s) 148, 533, 1047, 1293
MflI RGATCY 3 cut(s) 533, 1047, 1293
MluCI AATT 8 cut(s) 334, 620, 723, 1154, 1187, 1233, 1527, 1617
MlyI GAGTC 2 cut(s) 745, 1635
MmeI TCCRAC 1 cut(s) 1579
MnlI CCTC 9 cut(s) 75, 507, 705, 782, 863, 1279, 1302, 1545, 1731
Mph1103I ATGCAT 1 cut(s) 972
MroI TCCGGA 2 cut(s) 991, 1050
MroXI GAANNNNTTC 2 cut(s) 124, 289
MseI TTAA 6 cut(s) 36, 542, 806, 837, 1232, 1368
MspI CCGG 6 cut(s) 329, 362, 590, 992, 1051, 1380
MspR9I CCNGG 5 cut(s) 362, 363, 1199, 1431, 1733
MvaI CCWGG 3 cut(s) 1199, 1431, 1733
MwoI GCNNNNNNNGC 4 cut(s) 1079, 1454, 1508, 1690
NciI CCSGG 2 cut(s) 362, 363
NcoI CCATGG 1 cut(s) 581
NdeI CATATG 1 cut(s) 308
NdeII GATC 4 cut(s) 148, 533, 1047, 1293
NlaIV GGNNCC 6 cut(s) 434, 990, 996, 1049, 1214, 1671
NmuCI GTSAC 4 cut(s) 585, 654, 976, 1108
NsiI ATGCAT 1 cut(s) 972
NspI RCATGY 2 cut(s) 1326, 1515
NspV TTCGAA 1 cut(s) 283
PaeI GCATGC 1 cut(s) 1326
PagI TCATGA 2 cut(s) 1263, 1473
PdmI GAANNNNTTC 2 cut(s) 124, 289
Pfl23II CGTACG 1 cut(s) 1032
PflMI CCANNNNNTGG 1 cut(s) 972
PinAI ACCGGT 1 cut(s) 1379
PkrI GCNGC 5 cut(s) 13, 301, 895, 898, 928
PleI GAGTC 2 cut(s) 745, 1634
PpsI GAGTC 2 cut(s) 745, 1634
Ppu21I YACGTR 1 cut(s) 1249
PshBI ATTAAT 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 1197, 1429, 1731
PspEI GGTNACC 1 cut(s) 585
PspGI CCWGG 3 cut(s) 1197, 1429, 1731
PspLI CGTACG 1 cut(s) 1032
PspN4I GGNNCC 6 cut(s) 434, 990, 996, 1049, 1214, 1671
PspPI GGNCC 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 3 cut(s) 533, 1047, 1293
RsaI GTAC 7 cut(s) 174, 324, 452, 1034, 1214, 1340, 1707
RsaNI GTAC 7 cut(s) 173, 323, 451, 1033, 1213, 1339, 1706
SaqAI TTAA 6 cut(s) 36, 542, 806, 837, 1232, 1368
SatI GCNGC 5 cut(s) 12, 300, 894, 897, 927
Sau3AI GATC 4 cut(s) 148, 533, 1047, 1293
Sau96I GGNCC 1 cut(s) 432
ScaI AGTACT 1 cut(s) 324
SchI GAGTC 2 cut(s) 745, 1635
ScrFI CCNGG 5 cut(s) 362, 363, 1199, 1431, 1733
SexAI ACCWGGT 1 cut(s) 1731
SfaNI GCATC 2 cut(s) 650, 1257
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 1 cut(s) 432
SmaI CCCGGG 1 cut(s) 363
SnaBI TACGTA 1 cut(s) 1249
SphI GCATGC 1 cut(s) 1326
Sse9I AATT 8 cut(s) 334, 620, 723, 1154, 1187, 1233, 1527, 1617
SsiI CCGC 2 cut(s) 29, 1300
SspI AATATT 1 cut(s) 1680
SspMI CTAG 6 cut(s) 183, 191, 507, 1145, 1413, 1623
StyD4I CCNGG 5 cut(s) 360, 361, 1197, 1429, 1731
StyI CCWWGG 2 cut(s) 381, 581
TaaI ACNGT 7 cut(s) 93, 124, 1032, 1044, 1282, 1560, 1596
TaiI ACGT 1 cut(s) 1251
TaqI TCGA 5 cut(s) 128, 283, 673, 858, 1726
TaqII GACCGA 1 cut(s) 836
TasI AATT 8 cut(s) 334, 620, 723, 1154, 1187, 1233, 1527, 1617
TatI WGTACW 4 cut(s) 322, 450, 1338, 1705
Tru1I TTAA 6 cut(s) 36, 542, 806, 837, 1232, 1368
Tru9I TTAA 6 cut(s) 36, 542, 806, 837, 1232, 1368
TscAI CASTG 3 cut(s) 466, 1287, 1599
TseFI GTSAC 4 cut(s) 585, 654, 976, 1108
TseI GCWGC 5 cut(s) 11, 299, 893, 896, 926
Tsp45I GTSAC 4 cut(s) 585, 654, 976, 1108
TspDTI ATGAA 5 cut(s) 17, 761, 1462, 1552, 1633
TspGWI ACGGA 1 cut(s) 1050
TspMI CCCGGG 1 cut(s) 361
TspRI CASTG 3 cut(s) 466, 1287, 1599
Van91I CCANNNNNTGG 1 cut(s) 972
VpaK11BI GGWCC 1 cut(s) 432
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 6 cut(s) 620, 723, 1154, 1187, 1527, 1617
XbaI TCTAGA 1 cut(s) 1144
XceI RCATGY 2 cut(s) 1326, 1515
XmaI CCCGGG 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 2 cut(s) 124, 289
XspI CTAG 6 cut(s) 183, 191, 507, 1145, 1413, 1623
ZrmI AGTACT 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.