Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010275

Physical Location & Seq

Genomic Coordinates Forward (+)

22011305 .. 22020386

Gene Structure 9082 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G59820.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3642 bp

ATGGTTCGATCGGGTAGTTTTAGCGTCGATTCTTCGGCGACTCATCAACGGACTCCGTCTCGAACGGTTACGTTGGGTCATATTCAACCACAGGCTCCGACTTATCGTACCGTTTATTGTAATGATCGGGAATCTAATCAACCCGTCCGGTTCAAGGGTAATTCGATTTCTACAACAAAGTATAATGTTTTCACATTTCTGCCCAAGGGTCTGTTTGAACAGTTTAGGCGCATAGCGAACATATACTTTCTTGGAATTTCGTGTCTATCAATGACACCAATAAGTCCTGTGAGTCCAATAACAAATGTAGCTCCACTGTCAATGGTGCTTCTTGTTTCTCTGATTAAAGAGGCTTTCGAAGACTGGAAACGGTTTCAAAATGATATGTCCATAAATAATAGCACAGTGGAAATCTTACAAGATCAACAGTGGGTATCTATTCCGTGGAGAAAGTTGCAAGTTGGAGATATTGTCAAGATAAAAAAAGATGGATTTTTCCCAGCAGATATTCTTTTCATGTCCAGTACTAATTCTGATGGTATCTGCTACGTTGAGACTGCAAACCTAGATGGAGAAACAAATTTGAAAATAAGAAAGGCACTTGAAAGGACATGGGATTACTTGGTACCAGAGAAAGCTTATGAGTTTAAAGGTGAAATTCAGTGTGAGCAACCAAACAATTCTCTGTACACTTTTACTGGGAACCTTGTAGTCCAAAAGCAGACGTTACCTCTCTCTCCGGACCAGCTTTTATTGCGTGGATGCAGTCTTAGGAACACAGAATACATAGTTGGAGCTGTAGTTTTTACTGGTCATGAGACGAAGGTGATGATGAATGCCATGAATGCTCCTTCCAAAAGAAGTACGCTGGAAAAGAAGCTTGACAAACTCATTATTACAATATTTTGTGTCCTTGTCACGATGTGTCTGATTGGAGCTATAGGCTGTTCAATTGTGACGGATCGTGAGGACAAATACTTGGGTCTTCATAACTCAGATTGGGAATACCGCAATGGACTCATGATAGGATTTTTCACCTTTTTCACGCTAGTCACTCTGTTCTCATCAATTATACCAATCTCCCTTTATGTTTCTATTGAGATGATCAAGTTTATCCAGTCGACCCAGTTTATCAACCGCGATCTTAATATGTATCATGCTGAAACAAATACCCCTGCCTCGGCCAGGACTTCTAATTTGAACGAGGAGCTTGGACAGGTGGAATACATATTTTCCGATAAAACCGGAACACTGACAAGAAATTTGATGGAGTTCTTCAAATGTTCAATTGGAGGTGTAAGTTATGGATGTGGTGTCACTGAAATAGAAAAAGGAATCGCTCAGCGTCATGGCTTAAAAGTTCAAGAGGAGCAAAGGTCCACGGGTGCAATAAGAGAAAAAGGATTTAATTTTGATGATCCAAGGCTAATGCGGGGAGCTTGGAGAAACGAACCTAATCCTGATCTTTGCAAGGAACTGTTTAGATGCCTGGCCATCTGTCACACTGTCCTTCCTGAAGGTGATGAGTCCCCTGAGAAGATTGTTTATCAAGCTGCATCTCCAGATGAAGCTGCCTTAGTTACTGCTGCCAAGAATTTTGGATTCTTCTTTTATAGGCGTACTCCAACTATGGTGTATGTTCGCGAGTCTCATGTGGAGAAGATGGGAAAGATTCAAGACGTGGCCTATGAGATCCTAAACGTTCTCGAGTTCAACAGTACTAGGAAGCGCCAGTCTGTTGTATGTCGTTTCCCAGATGGAAGGCTTGTACTCTACTGTAAGGGCGCTGATAATGTTATCTTTGAGAGATTGGCTAATGGTATGGACGATGTTAGGAAAGTAACAAGAGAACATTTGGAACATTTTGGGTCTTCTGGATTGCGTACCCTTTGCCTAGCCTATAAAGATTTAAATCCTGAAACCTATGATAGTTGGAATGAAAAGTTCATCCAAGCCAAATCTGCATTACGAGACCGTGAGAAGAAGTTAGATGAGGTGGCAGAACTCATCGAGAAGGATCTTATCCTCATCGGATCAACTGCGATAGAAGACAAACTTCAGGAAGGGGTGCCTACCTGCATAGAAACATTATCAAGAGCTGGAATTAAGATCTGGGTGTTAACAGGGGACAAAATGGAAACGGCTATTAATATTGCATATGCATGCAACTTGATCAACAATGAAATGAAACAATTTGTCATTAGTTCTGAAACCGATGCAATCAGAGAAGCTGAAGAAAGGGGTGATCAAGTTGAGATTGCTCGTGTTATCAAAGAAGAAGTAAAGAGGGAGCTGAAAAAGAGTCTTGAGGAAGCTCAACACTCTTTGCATACCGTAGCTGGACCAAAATTGTCTCTCGTTATTGATGGCAAGTGTTTAATGTATGCATTAGACCCGAGTTTACGCGTGATGCTGCTCAGCTTGAGCTTGAACTGTACTTCAGTTGTATGCTGTCGTGTATCTCCGTTACAGAAAGCACAGGTGACAAGCTTGGTGAGAAAAGGGGCACAAAAGATTACTCTTAGTATTGGTGATGGGGCCAATGATGTTAGCATGATTCAAGCTGCTCATGTTGGTATAGGAATAAGTGGGATGGAGGGAATGCAAGCTGTGATGGCTAGTGATTTTGCCATTGCTCAGTTCAGATTTCTTACAGATTTACTTCTTGTCCATGGACGGTGGTCATATCTTAGAATCTGCAAGGTTGTCATGTATTTCTTCTACAAGAATCTTACCTTCACTTTGACTCAGTTTTGGTTCACATTTCGAACTGGATTTTCTGGGCAAAGGTTTTATGATGATTGGTTCCAGTCATTGTTTAATGTTGTATTTACAGCTCTTCCTGTCATCGTCCTTGGGCTGTTTGAGAAGGATGTGAGTGCATCCCTTTCGAAAAGATATCCAGAACTGTATAGAGAGGGAATACGTAACTCATTTTTCAAATGGAGAGTTGTAGCAGTATGGGCTACTTCTGCTGTGTATCAGTCTCTTGTCTGCTATCTCTTTGTGACAACCTCATCTTTCGGTGCTGTAAATTCATCAGGCAAAGTATTTGGCCTCTGGGATGTTAGCACAATGGTCTTCACATGTCTGGTGATTGCTGTGAATGTGCGCATTCTTTTGATGAGCAATTCCATTACCAGATGGCATTATATCACAGTCGGTGGGAGTATTCTTGCATGGCTCGTTTTTGCTTTCGTATACTGCGGGATTATGACACCACATGATAGGAATGAAAATGTCTACTTTGTCATATATGTTCTGATGAGTACATTCTATTTCTACTTCACATTGTTGCTTGTTCCCATTGTCTCTCTTCTAGGCGATTTCATCTTCCAAGGGGTGGAGAGATGGTTCTTCCCGTATGATTATCAGATCGTTCAAGAAATACATAGGCACGAGTCAGATGCAAGCAAGGCAGATCAGCTGGAGGTAGAGAATGAGCTCACACCTCAAGAGGCGAGAAGCTATGCGATATCACAATTGCCTCGGGAGCTCTCAAAGCACACTGGGTTTGCGTTTGATTCACCAGGTTATGAATCTTTCTTTGCGTCTCAGTTAGGGATATACGCGCCACAGAAGGCGTGGGATGTGGCAAGGAGAGCCAGTATGAGATCACGGCCAAAGGTACCGAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022622 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0048364 GO:0048367 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0097708 GO:0098791 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1213

Amino Acids

137.75

Weight (kDa)

7.84

Isoelectric Point (pI)

38.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	38 - 102	8.6e-26	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	490 - 688	2.7e-23	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	871 - 1121	1.2e-83	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59820
fragaria_vesca	FvH4_4g35440 FvH4_4g35440 FvH4_4g35440 FvH4_4g35440
malus_domestica	MD13G1083400.v1.1 MD16G1082100.v1.1
prunus_persica	Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1
pyrus_communis	pycom13g07180 pycom16g07110
rosa_chinensis	RchiOBHm_Chr4g0445071
rosa_laevigata	RLG00000005770
rosa_multiflora	Rmu_sc0003784.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385610
rosa_rugosa	Rorug04G0358500
rosa_samantha	Rh4AG419600 Rh4BG431300 Rh4CG445800
rosa_wichuraiana	Rw4G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3113
Acc36I	ACCTGC	1 cut(s)	2084
Acc65I	GGTACC	2 cut(s)	625, 3628
AccB1I	GGYRCC	3 cut(s)	625, 2068, 3628
AccB7I	CCANNNNNTGG	1 cut(s)	3343
AccI	GTMKAC	3 cut(s)	1121, 3201, 3243
AccII	CGCG	4 cut(s)	1140, 1644, 2406, 3572
AccIII	TCCGGA	1 cut(s)	739
AciI	CCGC	4 cut(s)	1009, 1138, 1432, 3207
AclI	AACGTT	1 cut(s)	1701
AclWI	GGATC	5 cut(s)	969, 1412, 1687, 2025, 2041
AcoI	YGGCCR	3 cut(s)	1182, 1491, 3620
AcsI	RAATTY	6 cut(s)	255, 580, 657, 1261, 1594, 3034
AcuI	CTGAAG	4 cut(s)	1536, 2042, 2253, 2424
AdeI	CACNNNGTG	1 cut(s)	924
AfiI	CCNNNNNNNGG	4 cut(s)	154, 1180, 1185, 3343
AflIII	ACRYGT	2 cut(s)	2404, 3086
AjiI	CACGTC	1 cut(s)	1681
AjnI	CCWGG	3 cut(s)	1184, 1488, 3529
AjuI	GAANNNNNNNTTGG	2 cut(s)	774, 806
AloI	GAACNNNNNNTCC	2 cut(s)	3338, 3370
Alw21I	GWGCWC	2 cut(s)	3447, 3498
Alw26I	GTCTC	9 cut(s)	63, 548, 812, 1653, 1965, 2358, 2991, 3316, 3558
AlwI	GGATC	5 cut(s)	969, 1412, 1687, 2025, 2041
Ama87I	CYCGRG	3 cut(s)	1706, 2395, 3489
Aor13HI	TCCGGA	1 cut(s)	739
AoxI	GGCC	6 cut(s)	1182, 1491, 1683, 2538, 3055, 3620
ApeKI	GCWGC	5 cut(s)	1553, 1571, 1586, 2413, 2564
ApoI	RAATTY	6 cut(s)	255, 580, 657, 1261, 1594, 3034
ArsI	GACNNNNNNTTYG	4 cut(s)	1180, 1212, 1246, 1278
AseI	ATTAAT	1 cut(s)	2148
Asp718I	GGTACC	2 cut(s)	625, 3628
AspLEI	GCGC	5 cut(s)	231, 1731, 1787, 3114, 3574
AspS9I	GGNCC	4 cut(s)	742, 1377, 2342, 2538
AsuII	TTCGAA	3 cut(s)	357, 2767, 2891
AvaI	CYCGRG	3 cut(s)	1706, 2395, 3489
AvaII	GGWCC	3 cut(s)	742, 1377, 2342
BaeGI	GKGCMC	1 cut(s)	2509
BalI	TGGCCA	1 cut(s)	1493
BanI	GGYRCC	3 cut(s)	625, 2068, 3628
BanII	GRGCYC	2 cut(s)	3447, 3498
BauI	CACGAG	2 cut(s)	2262, 3398
BbsI	GAAGAC	5 cut(s)	366, 977, 1863, 2055, 3073
Bbv12I	GWGCWC	2 cut(s)	3447, 3498
BbvI	GCAGC	5 cut(s)	1540, 1558, 1573, 2400, 2551
BceAI	ACGGC	2 cut(s)	2157, 3635
BcgI	CGANNNNNNTGC	4 cut(s)	2871, 2905, 3389, 3423
BciT130I	CCWGG	3 cut(s)	1186, 1490, 3531
BclI	TGATCA	3 cut(s)	1104, 2172, 2245
BcoDI	GTCTC	9 cut(s)	63, 548, 812, 1653, 1965, 2358, 2991, 3316, 3558
BfaI	CTAG	6 cut(s)	566, 1049, 1722, 1895, 2619, 3320
BfmI	CTRYAG	2 cut(s)	798, 939
BfoI	RGCGCY	2 cut(s)	1732, 1788
BfuAI	ACCTGC	1 cut(s)	2084
BglII	AGATCT	1 cut(s)	2109
BisI	GCNGC	5 cut(s)	1554, 1572, 1587, 2414, 2565
BlpI	GCTNAGC	2 cut(s)	1341, 2417
BlsI	GCNGC	5 cut(s)	1555, 1573, 1588, 2415, 2566
BmcAI	AGTACT	2 cut(s)	526, 1720
Bme1390I	CCNGG	3 cut(s)	1186, 1490, 3531
Bme18I	GGWCC	3 cut(s)	742, 1377, 2342
BmeT110I	CYCGRG	3 cut(s)	1706, 2395, 3489
BmgBI	CACGTC	1 cut(s)	1681
BmgT120I	GGNCC	4 cut(s)	742, 1377, 2342, 2538
BmiI	GGNNCC	7 cut(s)	96, 627, 704, 2070, 2539, 2807, 3630
BmrFI	CCNGG	3 cut(s)	1186, 1490, 3531
BmrI	ACTGGG	3 cut(s)	708, 1120, 3519
BmsI	GCATC	7 cut(s)	752, 1475, 1565, 2206, 2400, 2891, 3397
BmuI	ACTGGG	3 cut(s)	708, 1120, 3519
BoxI	GACNNNNGTC	1 cut(s)	2680
BpiI	GAAGAC	5 cut(s)	366, 977, 1863, 2055, 3073
BpmI	CTGGAG	2 cut(s)	1545, 3449
Bpu1102I	GCTNAGC	2 cut(s)	1341, 2417
Bpu14I	TTCGAA	3 cut(s)	357, 2767, 2891
BpuEI	CTTGAG	3 cut(s)	2327, 2443, 3438
BsaAI	YACGTR	1 cut(s)	2927
BsaBI	GATNNNNATC	1 cut(s)	1911
BsaI	GGTCTC	1 cut(s)	1965
BsaJI	CCNNGG	9 cut(s)	204, 443, 1179, 1380, 1421, 2671, 2854, 3337, 3488
BsaWI	WCCGGW	3 cut(s)	147, 739, 1244
Bsc4I	CCNNNNNNNGG	4 cut(s)	154, 1180, 1185, 3343
Bse3DI	GCAATG	2 cut(s)	1018, 2631
Bse8I	GATNNNNATC	1 cut(s)	1911
BseAI	TCCGGA	1 cut(s)	739
BseBI	CCWGG	3 cut(s)	1186, 1490, 3531
BseDI	CCNNGG	9 cut(s)	204, 443, 1179, 1380, 1421, 2671, 2854, 3337, 3488
BseGI	GGATG	8 cut(s)	767, 1313, 1947, 2598, 2878, 2882, 3070, 3595
BseJI	GATNNNNATC	1 cut(s)	1911
BseLI	CCNNNNNNNGG	4 cut(s)	154, 1180, 1185, 3343
BseMI	GCAATG	2 cut(s)	1018, 2631
BseMII	CTCAG	7 cut(s)	1008, 1355, 1524, 2431, 2651, 2762, 3569
BseRI	GAGGAG	2 cut(s)	1220, 1382
BseSI	GKGCMC	1 cut(s)	2509
BseXI	GCAGC	5 cut(s)	1540, 1558, 1573, 2400, 2551
BseYI	CCCAGC	1 cut(s)	499
Bsh1236I	CGCG	4 cut(s)	1140, 1644, 2406, 3572
Bsh1285I	CGRYCG	1 cut(s)	11
BshFI	GGCC	6 cut(s)	1184, 1493, 1685, 2540, 3057, 3622
BshNI	GGYRCC	3 cut(s)	625, 2068, 3628
BsiEI	CGRYCG	1 cut(s)	11
BsiHKAI	GWGCWC	2 cut(s)	3447, 3498
BsiHKCI	CYCGRG	3 cut(s)	1706, 2395, 3489
BsiSI	CCGG	3 cut(s)	148, 740, 1245
BslFI	GGGAC	2 cut(s)	1513, 2141
BslI	CCNNNNNNNGG	4 cut(s)	154, 1180, 1185, 3343
BsmAI	GTCTC	9 cut(s)	63, 548, 812, 1653, 1965, 2358, 2991, 3316, 3558
BsmBI	CGTCTC	3 cut(s)	63, 812, 3558
BsmFI	GGGAC	2 cut(s)	1513, 2141
BsmI	GAATGC	4 cut(s)	841, 850, 2607, 3114
BsnI	GGCC	6 cut(s)	1184, 1493, 1685, 2540, 3057, 3622
Bso31I	GGTCTC	1 cut(s)	1965
BsoBI	CYCGRG	3 cut(s)	1706, 2395, 3489
Bsp119I	TTCGAA	3 cut(s)	357, 2767, 2891
Bsp1286I	GDGCHC	3 cut(s)	2509, 3447, 3498
Bsp13I	TCCGGA	1 cut(s)	739
Bsp1407I	TGTACA	1 cut(s)	687
Bsp1720I	GCTNAGC	2 cut(s)	1341, 2417
Bsp19I	CCATGG	1 cut(s)	2671
Bsp68I	TCGCGA	1 cut(s)	1644
BspACI	CCGC	4 cut(s)	1009, 1138, 1432, 3207
BspANI	GGCC	6 cut(s)	1184, 1493, 1685, 2540, 3057, 3622
BspCNI	CTCAG	7 cut(s)	1007, 1354, 1525, 2430, 2650, 2761, 3568
BspEI	TCCGGA	1 cut(s)	739
BspFNI	CGCG	4 cut(s)	1140, 1644, 2406, 3572
BspHI	TCATGA	2 cut(s)	814, 1020
BspLI	GGNNCC	7 cut(s)	96, 627, 704, 2070, 2539, 2807, 3630
BspMI	ACCTGC	1 cut(s)	2084
BspPI	GGATC	5 cut(s)	969, 1412, 1687, 2025, 2041
BspQI	GCTCTTC	1 cut(s)	2844
BspT104I	TTCGAA	3 cut(s)	357, 2767, 2891
BspT107I	GGYRCC	3 cut(s)	625, 2068, 3628
BspTNI	GGTCTC	1 cut(s)	1965
BsrDI	GCAATG	2 cut(s)	1018, 2631
BsrGI	TGTACA	1 cut(s)	687
BssECI	CCNNGG	9 cut(s)	204, 443, 1179, 1380, 1421, 2671, 2854, 3337, 3488
BssNAI	GTATAC	1 cut(s)	3202
BssSI	CACGAG	2 cut(s)	2262, 3398
BssT1I	CCWWGG	5 cut(s)	204, 1421, 2671, 2854, 3337
Bst1107I	GTATAC	1 cut(s)	3202
Bst2BI	CACGAG	2 cut(s)	2262, 3398
Bst2UI	CCWGG	3 cut(s)	1186, 1490, 3531
Bst6I	CTCTTC	2 cut(s)	2844, 3321
BstAUI	TGTACA	1 cut(s)	687
BstBAI	YACGTR	1 cut(s)	2927
BstBI	TTCGAA	3 cut(s)	357, 2767, 2891
BstC8I	GCNNGC	3 cut(s)	2164, 2607, 3412
BstDSI	CCRYGG	3 cut(s)	443, 1380, 2671
BstENI	CCTNNNNNAGG	1 cut(s)	1183
BstF5I	GGATG	8 cut(s)	767, 1313, 1947, 2598, 2878, 2882, 3070, 3595
BstFNI	CGCG	4 cut(s)	1140, 1644, 2406, 3572
BstH2I	RGCGCY	2 cut(s)	1732, 1788
BstHHI	GCGC	5 cut(s)	231, 1731, 1787, 3114, 3574
BstMAI	GTCTC	9 cut(s)	63, 548, 812, 1653, 1965, 2358, 2991, 3316, 3558
BstMCI	CGRYCG	1 cut(s)	11
BstNI	CCWGG	3 cut(s)	1186, 1490, 3531
BstNSI	RCATGY	2 cut(s)	2166, 3090
BstPAI	GACNNNNGTC	1 cut(s)	2680
BstSCI	CCNGG	3 cut(s)	1184, 1488, 3529
BstSFI	CTRYAG	2 cut(s)	798, 939
BstSLI	GKGCMC	1 cut(s)	2509
BstSNI	TACGTA	1 cut(s)	2927
BstUI	CGCG	4 cut(s)	1140, 1644, 2406, 3572
BstV1I	GCAGC	5 cut(s)	1540, 1558, 1573, 2400, 2551
BstV2I	GAAGAC	5 cut(s)	366, 977, 1863, 2055, 3073
BstX2I	RGATCY	3 cut(s)	1692, 2017, 2109
BstYI	RGATCY	3 cut(s)	1692, 2017, 2109
BstZ17I	GTATAC	1 cut(s)	3202
BsuRI	GGCC	6 cut(s)	1184, 1493, 1685, 2540, 3057, 3622
BtgI	CCRYGG	3 cut(s)	443, 1380, 2671
BtrI	CACGTC	1 cut(s)	1681
BtsCI	GGATG	8 cut(s)	767, 1313, 1947, 2598, 2878, 2882, 3070, 3595
BtsIMutI	CAGTG	8 cut(s)	314, 411, 434, 668, 1250, 1317, 1503, 3507
BtuMI	TCGCGA	1 cut(s)	1644
BveI	ACCTGC	1 cut(s)	2084
Cac8I	GCNNGC	3 cut(s)	2164, 2607, 3412
CciI	TCATGA	2 cut(s)	814, 1020
CfoI	GCGC	5 cut(s)	231, 1731, 1787, 3114, 3574
Cfr13I	GGNCC	4 cut(s)	742, 1377, 2342, 2538
CseI	GACGC	3 cut(s)	13, 1334, 3540
CsiI	ACCWGGT	1 cut(s)	3529
DraI	TTTAAA	2 cut(s)	649, 1911
DraIII	CACNNNGTG	1 cut(s)	924
EaeI	YGGCCR	3 cut(s)	1182, 1491, 3620
Eam1104I	CTCTTC	2 cut(s)	2844, 3321
EarI	CTCTTC	2 cut(s)	2844, 3321
Ecl136II	GAGCTC	2 cut(s)	3445, 3496
Eco105I	TACGTA	1 cut(s)	2927
Eco130I	CCWWGG	5 cut(s)	204, 1421, 2671, 2854, 3337
Eco24I	GRGCYC	2 cut(s)	3447, 3498
Eco31I	GGTCTC	1 cut(s)	1965
Eco32I	GATATC	2 cut(s)	2900, 3477
Eco47I	GGWCC	3 cut(s)	742, 1377, 2342
Eco53kI	GAGCTC	2 cut(s)	3445, 3496
Eco57I	CTGAAG	4 cut(s)	1536, 2042, 2253, 2424
Eco88I	CYCGRG	3 cut(s)	1706, 2395, 3489
EcoICRI	GAGCTC	2 cut(s)	3445, 3496
EcoNI	CCTNNNNNAGG	1 cut(s)	1183
EcoRII	CCWGG	3 cut(s)	1184, 1488, 3529
EcoRV	GATATC	2 cut(s)	2900, 3477
EcoT14I	CCWWGG	5 cut(s)	204, 1421, 2671, 2854, 3337
EcoT22I	ATGCAT	2 cut(s)	2164, 2389
EcoT38I	GRGCYC	2 cut(s)	3447, 3498
ErhI	CCWWGG	5 cut(s)	204, 1421, 2671, 2854, 3337
Esp3I	CGTCTC	3 cut(s)	63, 812, 3558
FalI	AAGNNNNNCTT	2 cut(s)	2040, 2072
FaqI	GGGAC	2 cut(s)	1513, 2141
FauI	CCCGC	2 cut(s)	1425, 3200
FauNDI	CATATG	1 cut(s)	2158
FbaI	TGATCA	3 cut(s)	1104, 2172, 2245
FblI	GTMKAC	3 cut(s)	1121, 3201, 3243
Fnu4HI	GCNGC	5 cut(s)	1554, 1572, 1587, 2414, 2565
FokI	GGATG	8 cut(s)	774, 1320, 1934, 2605, 2869, 2885, 3077, 3602
FriOI	GRGCYC	2 cut(s)	3447, 3498
Fsp4HI	GCNGC	5 cut(s)	1554, 1572, 1587, 2414, 2565
FspAI	RTGCGCAY	1 cut(s)	3113
FspBI	CTAG	6 cut(s)	566, 1049, 1722, 1895, 2619, 3320
FspI	TGCGCA	1 cut(s)	3113
GlaI	GCGC	5 cut(s)	230, 1730, 1786, 3113, 3573
GluI	GCNGC	5 cut(s)	1554, 1572, 1587, 2414, 2565
GsaI	CCCAGC	1 cut(s)	503
GsuI	CTGGAG	2 cut(s)	1545, 3449
HaeII	RGCGCY	2 cut(s)	1732, 1788
HaeIII	GGCC	6 cut(s)	1184, 1493, 1685, 2540, 3057, 3622
HapII	CCGG	3 cut(s)	148, 740, 1245
HgaI	GACGC	3 cut(s)	13, 1334, 3540
HhaI	GCGC	5 cut(s)	231, 1731, 1787, 3114, 3574
Hin6I	GCGC	5 cut(s)	229, 1729, 1785, 3112, 3572
HinP1I	GCGC	5 cut(s)	229, 1729, 1785, 3112, 3572
HincII	GTYRAC	2 cut(s)	1122, 2121
HindII	GTYRAC	2 cut(s)	1122, 2121
HindIII	AAGCTT	3 cut(s)	636, 878, 2488
HpaI	GTTAAC	1 cut(s)	2121
HpaII	CCGG	3 cut(s)	148, 740, 1245
Hpy166II	GTNNAC	8 cut(s)	690, 1122, 1380, 2121, 2402, 2760, 3202, 3244
Hpy8I	GTNNAC	8 cut(s)	690, 1122, 1380, 2121, 2402, 2760, 3202, 3244
Hpy99I	CGWCG	1 cut(s)	29
HpyCH4IV	ACGT	6 cut(s)	71, 549, 725, 1680, 1701, 2926
HpySE526I	ACGT	6 cut(s)	71, 549, 725, 1680, 1701, 2926
HspAI	GCGC	5 cut(s)	229, 1729, 1785, 3112, 3572
Kpn2I	TCCGGA	1 cut(s)	739
KpnI	GGTACC	2 cut(s)	629, 3632
Ksp22I	TGATCA	3 cut(s)	1104, 2172, 2245
KspAI	GTTAAC	1 cut(s)	2121
LguI	GCTCTTC	1 cut(s)	2844
Lsp1109I	GCAGC	5 cut(s)	1540, 1558, 1573, 2400, 2551
LweI	GCATC	7 cut(s)	752, 1475, 1565, 2206, 2400, 2891, 3397
MabI	ACCWGGT	1 cut(s)	3529
MaeI	CTAG	6 cut(s)	566, 1049, 1722, 1895, 2619, 3320
MaeII	ACGT	6 cut(s)	71, 549, 725, 1680, 1701, 2926
MfeI	CAATTG	3 cut(s)	951, 1287, 3482
MflI	RGATCY	3 cut(s)	1692, 2017, 2109
MhlI	GDGCHC	3 cut(s)	2509, 3447, 3498
MlsI	TGGCCA	1 cut(s)	1493
MluI	ACGCGT	1 cut(s)	2404
MluNI	TGGCCA	1 cut(s)	1493
MlyI	GAGTC	9 cut(s)	34, 46, 301, 1011, 1535, 1655, 2311, 2740, 3410
MmeI	TCCRAC	5 cut(s)	122, 442, 772, 1649, 1913
Mox20I	TGGCCA	1 cut(s)	1493
Mph1103I	ATGCAT	2 cut(s)	2164, 2389
MroI	TCCGGA	1 cut(s)	739
MscI	TGGCCA	1 cut(s)	1493
MslI	CAYNNNNRTG	1 cut(s)	2161
Msp20I	TGGCCA	1 cut(s)	1493
MspA1I	CMGCKG	1 cut(s)	3427
MspI	CCGG	3 cut(s)	148, 740, 1245
MspR9I	CCNGG	3 cut(s)	1186, 1490, 3531
MunI	CAATTG	3 cut(s)	951, 1287, 3482
Mva1269I	GAATGC	4 cut(s)	841, 850, 2607, 3114
MvaI	CCWGG	3 cut(s)	1186, 1490, 3531
MvnI	CGCG	4 cut(s)	1140, 1644, 2406, 3572
NcoI	CCATGG	1 cut(s)	2671
NdeI	CATATG	1 cut(s)	2158
NlaIV	GGNNCC	7 cut(s)	96, 627, 704, 2070, 2539, 2807, 3630
NmeAIII	GCCGAG	1 cut(s)	1160
NmuCI	GTSAC	7 cut(s)	916, 955, 1051, 1315, 1499, 2482, 3007
NruI	TCGCGA	1 cut(s)	1644
NsbI	TGCGCA	1 cut(s)	3113
NsiI	ATGCAT	2 cut(s)	2164, 2389
NspI	RCATGY	2 cut(s)	2166, 3090
NspV	TTCGAA	3 cut(s)	357, 2767, 2891
PaeI	GCATGC	1 cut(s)	2166
PaeR7I	CTCGAG	1 cut(s)	1706
PagI	TCATGA	2 cut(s)	814, 1020
PciI	ACATGT	1 cut(s)	3086
PciSI	GCTCTTC	1 cut(s)	2844
PctI	GAATGC	4 cut(s)	841, 850, 2607, 3114
PflFI	GACNNNGTC	1 cut(s)	55
PflMI	CCANNNNNTGG	1 cut(s)	3343
PkrI	GCNGC	5 cut(s)	1555, 1573, 1588, 2415, 2566
Ple19I	CGATCG	1 cut(s)	11
PleI	GAGTC	9 cut(s)	34, 46, 300, 1011, 1534, 1654, 2310, 2740, 3409
PpsI	GAGTC	9 cut(s)	34, 46, 300, 1011, 1534, 1654, 2310, 2740, 3409
Ppu21I	YACGTR	1 cut(s)	2927
PscI	ACATGT	1 cut(s)	3086
PshAI	GACNNNNGTC	1 cut(s)	2680
PshBI	ATTAAT	1 cut(s)	2148
Psp124BI	GAGCTC	2 cut(s)	3447, 3498
Psp1406I	AACGTT	1 cut(s)	1701
Psp6I	CCWGG	3 cut(s)	1184, 1488, 3529
PspFI	CCCAGC	1 cut(s)	499
PspGI	CCWGG	3 cut(s)	1184, 1488, 3529
PspN4I	GGNNCC	7 cut(s)	96, 627, 704, 2070, 2539, 2807, 3630
PspPI	GGNCC	4 cut(s)	742, 1377, 2342, 2538
PsrI	GAACNNNNNNTAC	2 cut(s)	767, 799
PsuI	RGATCY	3 cut(s)	1692, 2017, 2109
PsyI	GACNNNGTC	1 cut(s)	55
PvuI	CGATCG	1 cut(s)	11
PvuII	CAGCTG	1 cut(s)	3427
RruI	TCGCGA	1 cut(s)	1644
RseI	CAYNNNNRTG	1 cut(s)	2161
SacI	GAGCTC	2 cut(s)	3447, 3498
SalI	GTCGAC	1 cut(s)	1120
SapI	GCTCTTC	1 cut(s)	2844
SatI	GCNGC	5 cut(s)	1554, 1572, 1587, 2414, 2565
Sau96I	GGNCC	4 cut(s)	742, 1377, 2342, 2538
ScaI	AGTACT	2 cut(s)	526, 1720
SchI	GAGTC	9 cut(s)	34, 46, 301, 1011, 1535, 1655, 2311, 2740, 3410
ScrFI	CCNGG	3 cut(s)	1186, 1490, 3531
SduI	GDGCHC	3 cut(s)	2509, 3447, 3498
SexAI	ACCWGGT	1 cut(s)	3529
SfaNI	GCATC	7 cut(s)	752, 1475, 1565, 2206, 2400, 2891, 3397
SfcI	CTRYAG	2 cut(s)	798, 939
Sfr274I	CTCGAG	1 cut(s)	1706
SfuI	TTCGAA	3 cut(s)	357, 2767, 2891
SinI	GGWCC	3 cut(s)	742, 1377, 2342
SlaI	CTCGAG	1 cut(s)	1706
SmiI	ATTTAAAT	1 cut(s)	1911
SmiMI	CAYNNNNRTG	1 cut(s)	2161
SmlI	CTYRAG	4 cut(s)	1706, 2306, 2422, 3453
SmoI	CTYRAG	4 cut(s)	1706, 2306, 2422, 3453
SnaBI	TACGTA	1 cut(s)	2927
SphI	GCATGC	1 cut(s)	2166
SsiI	CCGC	4 cut(s)	1009, 1138, 1432, 3207
SspI	AATATT	2 cut(s)	903, 2152
SspMI	CTAG	6 cut(s)	566, 1049, 1722, 1895, 2619, 3320
SstI	GAGCTC	2 cut(s)	3447, 3498
StyD4I	CCNGG	3 cut(s)	1184, 1488, 3529
StyI	CCWWGG	5 cut(s)	204, 1421, 2671, 2854, 3337
SwaI	ATTTAAAT	1 cut(s)	1911
TaiI	ACGT	6 cut(s)	74, 552, 728, 1683, 1704, 2929
TatI	WGTACW	6 cut(s)	524, 687, 1718, 1768, 2435, 3269
TscAI	CASTG	8 cut(s)	321, 411, 434, 668, 1257, 1324, 1510, 3514
TseFI	GTSAC	7 cut(s)	916, 955, 1051, 1315, 1499, 2482, 3007
TseI	GCWGC	5 cut(s)	1553, 1571, 1586, 2413, 2564
Tsp45I	GTSAC	7 cut(s)	916, 955, 1051, 1315, 1499, 2482, 3007
TspGWI	ACGGA	5 cut(s)	45, 64, 432, 974, 2454
TspRI	CASTG	8 cut(s)	321, 411, 434, 668, 1257, 1324, 1510, 3514
Tth111I	GACNNNGTC	1 cut(s)	55
Van91I	CCANNNNNTGG	1 cut(s)	3343
VpaK11BI	GGWCC	3 cut(s)	742, 1377, 2342
VspI	ATTAAT	1 cut(s)	2148
XagI	CCTNNNNNAGG	1 cut(s)	1183
XapI	RAATTY	6 cut(s)	255, 580, 657, 1261, 1594, 3034
XceI	RCATGY	2 cut(s)	2166, 3090
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3582
XhoI	CTCGAG	1 cut(s)	1706
XmiI	GTMKAC	3 cut(s)	1121, 3201, 3243
XspI	CTAG	6 cut(s)	566, 1049, 1722, 1895, 2619, 3320
ZrmI	AGTACT	2 cut(s)	526, 1720
Zsp2I	ATGCAT	2 cut(s)	2164, 2389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.