Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0010275

Physical Location & Seq

Genomic Coordinates Reverse (-)

53123845 .. 53129950

Gene Structure 6106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0358500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGCAATGGACCACAAGCTCGCCGTGATTCTCGATCACCCTCAAAACGACGCCGAATTTCTCCTCGTGAAGCAGACGCGCCCTCCCAAATTCGACGACGATGAGTACGACTCGTTCGTCGATTCCGACCTCTGGGACTTGCCCTCCGCGCAATTGAACCTTCTAGAAGGTCAATCCGAGTCCTCAGTCGCCGTTCAAGGCGCAGAGTCGTGGTCGGAGAAGATAGAGTTGACCAAATTTGACCTGGATTCGGCTCTCAATTCGATTTTGGAACAAGTAGGGTTTAAGGCAGCTGATGCTGGAGACTGGAAGCTGTGGAAGTATGTTGAGGAAGCTCAGTTTGGGCCTGGGCCGCCAATCAATTCGGTTTTTGTTAGGGGAAAATTGGTGAATGCAGATCAAACTTTACAAGAGACGTGTAAGTGGATGTCGGTCGAAAGCTGTCTAAGCTGGCTTCAGGAAGTGAAACCAAGATGTGAGCGTGTGGGGCCTTTGATAGTTGCTGGGCTTCTAAATGAATTTGCACAAACAAGAGAGTTGCAGATCCCATCGACCTTACCTTACCAGGAGTACCCTCCTGGTGTTGTGCTTGTACCTATGGGAAGCAGGACAGGAAAACCTTTTCGTACAACAAACCTGATTGTTTTTGCACCTAAAAATGTTCCAAATGATTCTGGAAAGAATGGTTTTATCGCGTGTGGAGATGCGTTGATAGTGGATCCTGGGTGTCAGTCTAAATTCCATGAAGAGCTCAGACAAATTGTAGCTGCTTTGCCGAGAAAGCTAATTGTGTTTGTTACTCATCATCATCGCGATCATGTGGATGGTCTTTCAGTTATTCAACGTTGCAATCCTGAGGCTACATTATTAGCACATGAAAATACCATGCGACGCATTGGGAAAGATGACTGGTCTCTTGGCTTTACTGCTATTTCTGGAACTGAAGAGATTTGCATTGGCGGTCAGCGGTTGATTGCCATTTTCTCTCCGGGACATACAGATGGCCATATGGGACTGCTTCATGCCAGTACTCACTCATTGATTGTAGGTGATCATTGTGTGGGTCAAGGTAGTGCCATACTGGATGTTACTTCCGGGGGAAATATGACTGATTACTTTCAATCAACATACAAATTTATGGAGATTTCCCCACATGCTTTGATCCCCATGCATGGGAGAGTTAACCTATGGCCAAAGCACATGCTTTGTGGATATCTCAGGAACCGTAGAGGTAGAGAAAGTTCCATCTTGAAAGCGATAGAAAATGGAGCTGAAACATTATTTGATATAGTTGCAAATGTATATTCTGAGGTTGACCGTAGCTTTTGGATTCCTGCTGCATCAAATGTGAGGCTTCACGTGGATCATCTAAGCCAGCAAGATAAGTTGCCAAAGGCATTTTCAATTCAGAAGTTCCAGAAAACTTGTAGACTGCATTTCTTCTCTCGATGGATGTGGGCTTACCTCAGTAACGGTGTCCTCGGAAATTGTTCAAAGCCAAGAATATCTCATCTTCTTGTTGCTGGTGTTGTTGCTAGCTCTGCCGTGTTGTACATGTTTAAAAGACGGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022622 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0048364 GO:0048367 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0097708 GO:0098791 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

524

Amino Acids

58.5

Weight (kDa)

6.29

Isoelectric Point (pI)

41.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	233 - 363	1.4e-06	Metallo-beta-lactamase superfamily
WHD_BLACT	PF17778	426 - 462	4.7e-06	Beta-lactamase associated winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59820
fragaria_vesca	FvH4_4g35440 FvH4_4g35440 FvH4_4g35440 FvH4_4g35440
malus_domestica	MD13G1083400.v1.1 MD16G1082100.v1.1
prunus_persica	Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1
pyrus_communis	pycom13g07180 pycom16g07110
rosa_chinensis	RchiOBHm_Chr4g0445071
rosa_laevigata	RLG00000005770
rosa_multiflora	Rmu_sc0003784.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385610
rosa_rugosa	Rorug04G0358500
rosa_samantha	Rh4AG419600 Rh4BG431300 Rh4CG445800
rosa_wichuraiana	Rw4G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	116
AccI	GTMKAC	1 cut(s)	1429
AccII	CGCG	4 cut(s)	79, 149, 695, 813
AciI	CCGC	4 cut(s)	147, 353, 960, 967
AclI	AACGTT	1 cut(s)	844
AclWI	GGATC	5 cut(s)	538, 713, 726, 1156, 1371
AcoI	YGGCCR	2 cut(s)	1003, 1190
AcsI	RAATTY	6 cut(s)	56, 89, 236, 518, 737, 1133
AcuI	CTGAAG	2 cut(s)	440, 963
AcvI	CACGTG	1 cut(s)	1360
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	6 cut(s)	107, 572, 594, 628, 1030, 1553
AfiI	CCNNNNNNNGG	4 cut(s)	132, 250, 1172, 1173
AflIII	ACRYGT	2 cut(s)	416, 1554
AgsI	TTSAA	7 cut(s)	157, 197, 842, 1121, 1252, 1404, 1494
AjiI	CACGTC	1 cut(s)	417
AjnI	CCWGG	5 cut(s)	243, 346, 564, 577, 721
AjuI	GAANNNNNNNTTGG	4 cut(s)	324, 356, 1384, 1416
Alw21I	GWGCWC	1 cut(s)	753
Alw26I	GTCTC	3 cut(s)	297, 407, 918
AlwI	GGATC	5 cut(s)	538, 713, 726, 1156, 1371
AoxI	GGCC	5 cut(s)	344, 350, 488, 1003, 1190
ApeKI	GCWGC	3 cut(s)	290, 767, 1337
ApoI	RAATTY	6 cut(s)	56, 89, 236, 518, 737, 1133
Asp700I	GAANNNNTTC	1 cut(s)	1489
AspLEI	GCGC	3 cut(s)	81, 151, 203
AspS9I	GGNCC	4 cut(s)	10, 344, 350, 488
AsuC2I	CCSGG	2 cut(s)	990, 1096
AsuHPI	GGTGA	3 cut(s)	29, 400, 1061
AsuNHI	GCTAGC	1 cut(s)	1535
AvaII	GGWCC	1 cut(s)	10
AxyI	CCTNAGG	1 cut(s)	855
BalI	TGGCCA	2 cut(s)	1005, 1192
BamHI	GGATCC	1 cut(s)	718
BanII	GRGCYC	1 cut(s)	753
BauI	CACGAG	1 cut(s)	65
BbrPI	CACGTG	1 cut(s)	1360
Bbv12I	GWGCWC	1 cut(s)	753
BbvI	GCAGC	3 cut(s)	302, 754, 1324
BccI	CCATC	5 cut(s)	556, 818, 995, 1253, 1443
BceAI	ACGGC	3 cut(s)	8, 176, 1529
BciT130I	CCWGG	5 cut(s)	245, 348, 566, 579, 723
BclI	TGATCA	1 cut(s)	1051
BcnI	CCSGG	2 cut(s)	990, 1096
BcoDI	GTCTC	3 cut(s)	297, 407, 918
BfaI	CTAG	2 cut(s)	164, 1536
BisI	GCNGC	4 cut(s)	291, 353, 768, 1338
BlsI	GCNGC	4 cut(s)	292, 354, 769, 1339
BmcAI	AGTACT	1 cut(s)	1030
Bme1390I	CCNGG	7 cut(s)	245, 348, 566, 579, 723, 990, 1096
Bme18I	GGWCC	1 cut(s)	10
BmgBI	CACGTC	1 cut(s)	417
BmgT120I	GGNCC	4 cut(s)	10, 344, 350, 488
BmiI	GGNNCC	3 cut(s)	489, 720, 1223
BmrFI	CCNGG	7 cut(s)	245, 348, 566, 579, 723, 990, 1096
BmsI	GCATC	3 cut(s)	286, 694, 1349
BmtI	GCTAGC	1 cut(s)	1539
BplI	GAGNNNNNCTC	2 cut(s)	95, 127
BpmI	CTGGAG	1 cut(s)	321
BpuMI	CCSGG	2 cut(s)	990, 1096
BsaAI	YACGTR	1 cut(s)	1360
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	1 cut(s)	918
BsaJI	CCNNGG	4 cut(s)	347, 722, 1095, 1480
BsaXI	ACNNNNNCTCC	4 cut(s)	67, 97, 128, 158
Bsc4I	CCNNNNNNNGG	4 cut(s)	132, 250, 1172, 1173
Bse1I	ACTGG	4 cut(s)	311, 914, 1026, 1086
Bse21I	CCTNAGG	1 cut(s)	855
Bse3DI	GCAATG	1 cut(s)	12
BseBI	CCWGG	5 cut(s)	245, 348, 566, 579, 723
BseDI	CCNNGG	4 cut(s)	347, 722, 1095, 1480
BseGI	GGATG	4 cut(s)	432, 829, 1090, 1458
BseLI	CCNNNNNNNGG	4 cut(s)	132, 250, 1172, 1173
BseMI	GCAATG	1 cut(s)	12
BseMII	CTCAG	7 cut(s)	198, 350, 766, 846, 1231, 1299, 1480
BseNI	ACTGG	4 cut(s)	311, 914, 1026, 1086
BseRI	GAGGAG	1 cut(s)	53
BseXI	GCAGC	3 cut(s)	302, 754, 1324
BseYI	CCCAGC	1 cut(s)	503
Bsh1236I	CGCG	4 cut(s)	79, 149, 695, 813
Bsh1285I	CGRYCG	1 cut(s)	435
BshFI	GGCC	5 cut(s)	346, 352, 490, 1005, 1192
BsiEI	CGRYCG	1 cut(s)	435
BsiHKAI	GWGCWC	1 cut(s)	753
BsiSI	CCGG	2 cut(s)	989, 1095
BslFI	GGGAC	3 cut(s)	149, 1005, 1026
BslI	CCNNNNNNNGG	4 cut(s)	132, 250, 1172, 1173
BsmAI	GTCTC	3 cut(s)	297, 407, 918
BsmBI	CGTCTC	1 cut(s)	407
BsmFI	GGGAC	3 cut(s)	149, 1005, 1026
BsmI	GAATGC	1 cut(s)	397
BsnI	GGCC	5 cut(s)	346, 352, 490, 1005, 1192
Bso31I	GGTCTC	1 cut(s)	918
Bsp1286I	GDGCHC	1 cut(s)	753
Bsp1407I	TGTACA	1 cut(s)	1551
Bsp143I	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
Bsp68I	TCGCGA	1 cut(s)	813
BspACI	CCGC	4 cut(s)	147, 353, 960, 967
BspANI	GGCC	5 cut(s)	346, 352, 490, 1005, 1192
BspCNI	CTCAG	7 cut(s)	197, 349, 765, 847, 1230, 1300, 1479
BspFNI	CGCG	4 cut(s)	79, 149, 695, 813
BspLI	GGNNCC	3 cut(s)	489, 720, 1223
BspOI	GCTAGC	1 cut(s)	1539
BspPI	GGATC	5 cut(s)	538, 713, 726, 1156, 1371
BspQI	GCTCTTC	1 cut(s)	741
BspTNI	GGTCTC	1 cut(s)	918
BsrDI	GCAATG	1 cut(s)	12
BsrGI	TGTACA	1 cut(s)	1551
BsrI	ACTGG	4 cut(s)	311, 914, 1026, 1086
BssECI	CCNNGG	4 cut(s)	347, 722, 1095, 1480
BssMI	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	65
Bst2BI	CACGAG	1 cut(s)	65
Bst2UI	CCWGG	5 cut(s)	245, 348, 566, 579, 723
Bst4CI	ACNGT	3 cut(s)	1226, 1319, 1475
Bst6I	CTCTTC	2 cut(s)	741, 939
BstACI	GRCGYC	1 cut(s)	51
BstAPI	GCANNNNNTGC	1 cut(s)	296
BstAUI	TGTACA	1 cut(s)	1551
BstBAI	YACGTR	1 cut(s)	1360
BstC8I	GCNNGC	4 cut(s)	21, 452, 1376, 1537
BstDEI	CTNAG	9 cut(s)	184, 336, 446, 752, 855, 1217, 1308, 1370, 1466
BstF5I	GGATG	4 cut(s)	432, 829, 1090, 1458
BstFNI	CGCG	4 cut(s)	79, 149, 695, 813
BstHHI	GCGC	3 cut(s)	81, 151, 203
BstKTI	GATC	8 cut(s)	37, 400, 546, 721, 817, 1054, 1164, 1366
BstMAI	GTCTC	3 cut(s)	297, 407, 918
BstMBI	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
BstMCI	CGRYCG	1 cut(s)	435
BstMWI	GCNNNNNNNGC	7 cut(s)	148, 296, 352, 447, 487, 781, 1541
BstNI	CCWGG	5 cut(s)	245, 348, 566, 579, 723
BstNSI	RCATGY	3 cut(s)	1157, 1204, 1558
BstSCI	CCNGG	7 cut(s)	243, 346, 564, 577, 721, 988, 1094
BstUI	CGCG	4 cut(s)	79, 149, 695, 813
BstV1I	GCAGC	3 cut(s)	302, 754, 1324
BstX2I	RGATCY	2 cut(s)	543, 718
BstYI	RGATCY	2 cut(s)	543, 718
Bsu36I	CCTNAGG	1 cut(s)	855
BsuRI	GGCC	5 cut(s)	346, 352, 490, 1005, 1192
BtgZI	GCGATG	1 cut(s)	794
BtrI	CACGTC	1 cut(s)	417
BtsCI	GGATG	4 cut(s)	432, 829, 1090, 1458
BtuMI	TCGCGA	1 cut(s)	813
Cac8I	GCNNGC	4 cut(s)	21, 452, 1376, 1537
CfoI	GCGC	3 cut(s)	81, 151, 203
Cfr13I	GGNCC	4 cut(s)	10, 344, 350, 488
CseI	GACGC	3 cut(s)	59, 85, 900
Csp6I	GTAC	6 cut(s)	106, 571, 593, 627, 1029, 1552
CspCI	CAANNNNNGTGG	2 cut(s)	1140, 1175
CviQI	GTAC	6 cut(s)	106, 571, 593, 627, 1029, 1552
DdeI	CTNAG	9 cut(s)	184, 336, 446, 752, 855, 1217, 1308, 1370, 1466
DpnI	GATC	8 cut(s)	36, 399, 545, 720, 816, 1053, 1163, 1365
DpnII	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
DraI	TTTAAA	1 cut(s)	1561
DrdI	GACNNNNNNGTC	1 cut(s)	116
DseDI	GACNNNNNNGTC	1 cut(s)	116
EaeI	YGGCCR	2 cut(s)	1003, 1190
Eam1104I	CTCTTC	2 cut(s)	741, 939
EarI	CTCTTC	2 cut(s)	741, 939
Ecl136II	GAGCTC	1 cut(s)	751
Eco24I	GRGCYC	1 cut(s)	753
Eco31I	GGTCTC	1 cut(s)	918
Eco32I	GATATC	1 cut(s)	1214
Eco47I	GGWCC	1 cut(s)	10
Eco53kI	GAGCTC	1 cut(s)	751
Eco57I	CTGAAG	2 cut(s)	440, 963
Eco72I	CACGTG	1 cut(s)	1360
Eco81I	CCTNAGG	1 cut(s)	855
EcoICRI	GAGCTC	1 cut(s)	751
EcoO109I	RGGNCCY	1 cut(s)	488
EcoRII	CCWGG	5 cut(s)	243, 346, 564, 577, 721
EcoRV	GATATC	1 cut(s)	1214
EcoT22I	ATGCAT	1 cut(s)	1173
EcoT38I	GRGCYC	1 cut(s)	753
Esp3I	CGTCTC	1 cut(s)	407
FaqI	GGGAC	3 cut(s)	149, 1005, 1026
FauNDI	CATATG	1 cut(s)	1008
FbaI	TGATCA	1 cut(s)	1051
FblI	GTMKAC	1 cut(s)	1429
Fnu4HI	GCNGC	4 cut(s)	291, 353, 768, 1338
FokI	GGATG	4 cut(s)	439, 836, 1097, 1465
FriOI	GRGCYC	1 cut(s)	753
Fsp4HI	GCNGC	4 cut(s)	291, 353, 768, 1338
FspBI	CTAG	2 cut(s)	164, 1536
GlaI	GCGC	3 cut(s)	80, 150, 202
GluI	GCNGC	4 cut(s)	291, 353, 768, 1338
GsaI	CCCAGC	1 cut(s)	507
GsuI	CTGGAG	1 cut(s)	321
HaeIII	GGCC	5 cut(s)	346, 352, 490, 1005, 1192
HapII	CCGG	2 cut(s)	989, 1095
HgaI	GACGC	3 cut(s)	59, 85, 900
HhaI	GCGC	3 cut(s)	81, 151, 203
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	3 cut(s)	79, 149, 201
HinP1I	GCGC	3 cut(s)	79, 149, 201
HincII	GTYRAC	3 cut(s)	231, 1183, 1315
HindII	GTYRAC	3 cut(s)	231, 1183, 1315
HinfI	GANTC	8 cut(s)	28, 110, 122, 179, 206, 248, 671, 1330
HpaI	GTTAAC	1 cut(s)	1183
HpaII	CCGG	2 cut(s)	989, 1095
HphI	GGTGA	3 cut(s)	29, 400, 1061
Hpy166II	GTNNAC	4 cut(s)	231, 1183, 1315, 1430
Hpy188I	TCNGA	7 cut(s)	127, 178, 217, 755, 1309, 1410, 1484
Hpy8I	GTNNAC	4 cut(s)	231, 1183, 1315, 1430
Hpy99I	CGWCG	5 cut(s)	53, 98, 101, 122, 894
HpyAV	CCTTC	2 cut(s)	161, 170
HpyCH4III	ACNGT	3 cut(s)	1226, 1319, 1475
HpyCH4IV	ACGT	3 cut(s)	416, 844, 1359
HpyF10VI	GCNNNNNNNGC	7 cut(s)	148, 296, 352, 447, 487, 781, 1541
HpyF3I	CTNAG	9 cut(s)	184, 336, 446, 752, 855, 1217, 1308, 1370, 1466
HpySE526I	ACGT	3 cut(s)	416, 844, 1359
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	3 cut(s)	79, 149, 201
Ksp22I	TGATCA	1 cut(s)	1051
KspAI	GTTAAC	1 cut(s)	1183
Kzo9I	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	1 cut(s)	1268
Lsp1109I	GCAGC	3 cut(s)	302, 754, 1324
LweI	GCATC	3 cut(s)	286, 694, 1349
MaeI	CTAG	2 cut(s)	164, 1536
MaeII	ACGT	3 cut(s)	416, 844, 1359
MaeIII	GTNAC	3 cut(s)	796, 1087, 1469
MalI	GATC	8 cut(s)	36, 399, 545, 720, 816, 1053, 1163, 1365
MboI	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
MboII	GAAGA	5 cut(s)	232, 758, 956, 1432, 1505
MfeI	CAATTG	1 cut(s)	152
MflI	RGATCY	2 cut(s)	543, 718
MhlI	GDGCHC	1 cut(s)	753
MlsI	TGGCCA	2 cut(s)	1005, 1192
MluNI	TGGCCA	2 cut(s)	1005, 1192
MlyI	GAGTC	3 cut(s)	104, 188, 215
MmeI	TCCRAC	2 cut(s)	150, 195
Mox20I	TGGCCA	2 cut(s)	1005, 1192
Mph1103I	ATGCAT	1 cut(s)	1173
MroXI	GAANNNNTTC	1 cut(s)	1489
MscI	TGGCCA	2 cut(s)	1005, 1192
MseI	TTAA	3 cut(s)	285, 1182, 1560
MslI	CAYNNNNRTG	2 cut(s)	822, 999
Msp20I	TGGCCA	2 cut(s)	1005, 1192
MspA1I	CMGCKG	2 cut(s)	293, 967
MspI	CCGG	2 cut(s)	989, 1095
MspR9I	CCNGG	7 cut(s)	245, 348, 566, 579, 723, 990, 1096
MunI	CAATTG	1 cut(s)	152
Mva1269I	GAATGC	1 cut(s)	397
MvaI	CCWGG	5 cut(s)	245, 348, 566, 579, 723
MvnI	CGCG	4 cut(s)	79, 149, 695, 813
MwoI	GCNNNNNNNGC	7 cut(s)	148, 296, 352, 447, 487, 781, 1541
NciI	CCSGG	2 cut(s)	990, 1096
NdeI	CATATG	1 cut(s)	1008
NdeII	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
NheI	GCTAGC	1 cut(s)	1535
NlaIV	GGNNCC	3 cut(s)	489, 720, 1223
NmeAIII	GCCGAG	1 cut(s)	801
NruI	TCGCGA	1 cut(s)	813
NsiI	ATGCAT	1 cut(s)	1173
NspI	RCATGY	3 cut(s)	1157, 1204, 1558
PciI	ACATGT	1 cut(s)	1554
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	3 cut(s)	105, 114, 123
PctI	GAATGC	1 cut(s)	397
PdmI	GAANNNNTTC	1 cut(s)	1489
PfeI	GAWTC	5 cut(s)	28, 122, 248, 671, 1330
PfoI	TCCNGGA	1 cut(s)	988
PkrI	GCNGC	4 cut(s)	292, 354, 769, 1339
PleI	GAGTC	3 cut(s)	104, 187, 214
PmaCI	CACGTG	1 cut(s)	1360
PmlI	CACGTG	1 cut(s)	1360
PpsI	GAGTC	3 cut(s)	104, 187, 214
Ppu21I	YACGTR	1 cut(s)	1360
PscI	ACATGT	1 cut(s)	1554
Psp124BI	GAGCTC	1 cut(s)	753
Psp1406I	AACGTT	1 cut(s)	844
Psp6I	CCWGG	5 cut(s)	243, 346, 564, 577, 721
PspCI	CACGTG	1 cut(s)	1360
PspFI	CCCAGC	1 cut(s)	503
PspGI	CCWGG	5 cut(s)	243, 346, 564, 577, 721
PspN4I	GGNNCC	3 cut(s)	489, 720, 1223
PspPI	GGNCC	4 cut(s)	10, 344, 350, 488
PsrI	GAACNNNNNNTAC	4 cut(s)	98, 130, 1225, 1257
PsuI	RGATCY	2 cut(s)	543, 718
PvuII	CAGCTG	1 cut(s)	293
RruI	TCGCGA	1 cut(s)	813
RsaI	GTAC	6 cut(s)	107, 572, 594, 628, 1030, 1553
RsaNI	GTAC	6 cut(s)	106, 571, 593, 627, 1029, 1552
RseI	CAYNNNNRTG	2 cut(s)	822, 999
SacI	GAGCTC	1 cut(s)	753
SapI	GCTCTTC	1 cut(s)	741
SaqAI	TTAA	3 cut(s)	285, 1182, 1560
SatI	GCNGC	4 cut(s)	291, 353, 768, 1338
Sau3AI	GATC	8 cut(s)	34, 397, 543, 718, 814, 1051, 1161, 1363
Sau96I	GGNCC	4 cut(s)	10, 344, 350, 488
ScaI	AGTACT	1 cut(s)	1030
SchI	GAGTC	3 cut(s)	104, 188, 215
ScrFI	CCNGG	7 cut(s)	245, 348, 566, 579, 723, 990, 1096
SduI	GDGCHC	1 cut(s)	753
SfaNI	GCATC	3 cut(s)	286, 694, 1349
SinI	GGWCC	1 cut(s)	10
SmiMI	CAYNNNNRTG	2 cut(s)	822, 999
SsiI	CCGC	4 cut(s)	147, 353, 960, 967
SspMI	CTAG	2 cut(s)	164, 1536
SstI	GAGCTC	1 cut(s)	753
StyD4I	CCNGG	7 cut(s)	243, 346, 564, 577, 721, 988, 1094
TaaI	ACNGT	3 cut(s)	1226, 1319, 1475
TaiI	ACGT	3 cut(s)	419, 847, 1362
TaqI	TCGA	7 cut(s)	33, 93, 120, 263, 435, 551, 1447
TaqII	GACCGA	1 cut(s)	421
TatI	WGTACW	2 cut(s)	1028, 1551
TauI	GCSGC	1 cut(s)	355
TfiI	GAWTC	5 cut(s)	28, 122, 248, 671, 1330
Tru1I	TTAA	3 cut(s)	285, 1182, 1560
Tru9I	TTAA	3 cut(s)	285, 1182, 1560
TseI	GCWGC	3 cut(s)	290, 767, 1337
TspDTI	ATGAA	4 cut(s)	531, 759, 891, 1010
VpaK11BI	GGWCC	1 cut(s)	10
XapI	RAATTY	6 cut(s)	56, 89, 236, 518, 737, 1133
XbaI	TCTAGA	1 cut(s)	163
XceI	RCATGY	3 cut(s)	1157, 1204, 1558
XcmI	CCANNNNNNNNNTGG	1 cut(s)	241
XmiI	GTMKAC	1 cut(s)	1429
XmnI	GAANNNNTTC	1 cut(s)	1489
XspI	CTAG	2 cut(s)	164, 1536
ZrmI	AGTACT	1 cut(s)	1030
Zsp2I	ATGCAT	1 cut(s)	1173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.