Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010275

Physical Location & Seq

Genomic Coordinates Forward (+)

5759979 .. 5771453

Gene Structure 11475 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1082100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3675 bp

ATGAATGGATGGAATCGGTCCTCGAGAGGAGCGAGGCTGGGTGGTCGCAATTCGAGTTCGCAGCCCGAGCGGACGTCGACGACTCGGACGGTTCGGCTCGGGAGAGTCCAGCCACAGGCGCCGGGTCACCGCACTATTTTCTGCAACGATCGCGAGGCTAATATTCCAGTCAGATTCCCGGGGAATTCTATATCAACTACAAAGTACAACTTCTTTACTTTTTTGCCAAAAGGACTCTTTGAACAGTTCAGGCGGGTAGCTAACCTGTACTTTCTTACAATCTCAATTCTATCAACAACGCCAATCAGTCCTGTGCATCCTGTAACCAATGTGGTGCCTCTGAGTTTGGTGCTTTTTGTTTCTCTTGTTAAGGAAGCATTTGAGGATTGGAAGCGCTTCCAGAATGATATGACCATAAATAATGCTTCTGTTGATGTCTTGCAAGATCAAAAATGGGAAAGTATTCCCTGGAAAAAGTTGCAGGTTGGAGATATTGTCAGGATCAAGCAGAATGGATATTTCCCTGCAGATCTGCTTTTTCTTGCTGGTACAAACCCCGATGGTGTCTGCTACATTGAGACTGCAAATTTGGACGGGGAAACTAATTTGAAGATCCGAAAAGCATTAGAAAAGACTTGGGATTACTCGTCTCCTGAGAAAGCATCTGAATTCAAAGGAGAAGTGCAGTGTGAACAACCTAACAATTCATTGTACACCTTCACTGGAAATCTCATTATTGACAAGCAGACGTTACCTCTATCTCCAAACCAACTTTTGCTGCGAGGTTGCAGTCTCAGGAATACTGAATACATTGTTGCGACTGTTATATTTACAGGTCATGAAACCAAGGTTATGATGAATGCCATGAATGTTCCTTCGAAAAGAAGTACACTGGAGAGGAAACTTGACAAACTGATACTCGCACTTTTTGCTTTTCTCTTTATGTTGTGTTTGATTGGAGCTATTGGCAGTGGTGTATTCATCAACGACAAGTACTACTACTTAGGTCTCCGGGGGAAAAAGAATGCCGGCTCGTCATACAGCTCATTTGACCCTAATAACCGTTTTCTGGTTATCCTTCTGACGATGTTTACCCTCATTACTTTGTACTCAACAATAATCCCTATTTCTCTTTATGTTTCCATTGAGATGATCAAATTTATCCAGTCTACCCAATATATCAATAATGACTTGCGTATGTACCATGTCGAAAGCAATACCCCTGCTTTGGCTAGGACTTCGAATTTGAATGAGGAACTTGGACAGGTGGAATACATCTTTTCTGATAAAACTGGAACTCTAACAAGAAACTTGATGGAGTTCTTTAAGTGTTCAATTGGAGGGGAGGTCTATGGAACTGGTATCACTGAAATTGAAAGGGGAATAGCAGAGCGGAATGGTATAAAACTTGATGAGGGGTACAATTCAGGCAATGCAGCCCATGAGAAGGGATTTAACTTTGATGATCCTAAGCTTATGCGAGGAGGTTGGAGGAATGAGCCTAATCCTGATCTTTGTAAGGAATTCTTCAGATGCCTTGCTATATGTCACACGGTGCTTCCTGAGGGTGATGAGTCCCCGGAAAAAATCACATATCAAGCTGCATCCCCTGATGAGTCTGCTTTGGTAATTGCTGCAAAGAACTTCGGCTTCTTCTTTTACAGACGTACACCGACAACAATTTATGTGCGTGAATCTCACACTGAGAAACTCGGTAAAGTCCAAGATGTGTCTTATGAGATTCTCAATGTACTTGAATTTAATAGTACGAGGAAGCGTCAGTCTGTTGTATGCCGTTACACGGATGGCAGGCTTGTTTTGTACTGCAAGGGTGCTGATAATGTAATCTACGAGAGATTGGCAGATGGACAAGGTGATTTGAAAAAAGTATCTAGGGAACACTTGGAATTTTTTGGATCTGCTGGATTACGTACTCTTTGCCTGGCCTATAGAGATTTAAGTCCTGATACATATGAAAGCTGGAATGAGAAATTTATTCAGGCTAAATCCTCTCTAAGGGATCGTGAAAAGAAGTTGGATGAGGTGGCAGAACTCATAGAGAAAGATCTCATTTTGATTGGATGCACTGCCATTGAAGACAAGCTTCAAGAAGGAGTTCCAAATTGCATAGAGACTCTATCAAGAGCTGGTATTAAGATTTGGGTGCTAACGGGGGACAAGATGGAAACTGCAATAAATATAGCTTATGCATGCAATTTGATCAACAATGAAATGAAACAGTTCATTATCAGTTCAGAAACTGATTTGATTAGGGAAGTTGAGGACAGGGGAGACCAAGTGGAAATTGCACGTGTCATTAGAGAAGAAGTAAAAAAACAACTAAAGAGGTGCCTTGAGGAAGCGCAGCACTATCTTTACAGTGTGTCTCACCCAAAACTAGCACTTGTAATAGATGGGAAGTGCTTGATGTATGCCTTAGACCCCATTCTACGAGTGACGCTGCTGAACTTGAGCTTGAATTGTAACTCAGTTGTTTGCTGTCGAGTTTCCCCTTTACAGAAAGCACAGGTGACAAGTATGGTAAAAAAAGGTGCAAAGAAGATTACACTGAGCATTGGTGATGGTGCCAATGATGTGAGCATGATCCAAGCTGCTCATGTTGGCGTTGGAATAAGTGGGCAAGAAGGAATGCAAGCAGTTATGGCTAGTGATTTTGCTATTGCCCAGTTCCGTTTTCTTACTGATTTACTGCTTGTGCATGGACGGTGGTCATATATTAGATTATGCAAGGTTATTACATACTTCTTTTACAAGAATCTTACATTCACGTTGACTCAATTTTGGTTCACCTTTCAAACTGGATTTTCTGGTCAAAGATTCTACGATGATTGGTTTCAGTCATTGTATAATGTCATATTCACAGCCTTGCCTGTAATCATCGTCGGGCTTTTTGACAAGGATGTCAGTGCAGCCCTCTCCAAGAAGTACCCTGAACTATACAAGGAGGGAATAAGAAATGCATTTTTCAAATGGAGGGTTGTGGCCGTATGGGGCTTCTTCTCTGTTTACCAATCTTTAATCTTCTATTATTTTGTCACTTCCTCGAGTGATCGTGCTCAAAATTCATCCGGAAAGATGTTTGGACTTTGGGATGTCAGCACAATGGCCTTTACTTGTGTTGTAGTGACGGTCAACTTTCGTTTGATTATGATGTGCAATTCAATCACAAGGTGGCATTATATTAGTGTTGGAGGAAGCATTGCAGCGTGGTTTATATTTATCTTCATATATTGTATCATAGACCAGAAGAAAAATCTTTATTACGTCATATACGTCCTGATGAGTACCTTCTATTTCTACTTTACACTTCTGCTAGTCCCAGTTTTTGCACTTTTTGGCGACTTCGTTTACCAAGGGATCCAGAGATGGTTCTTCCCATTCGATTATCAAATAATTCAGGAAGTTCACAGGCATGAGCCGGAAGGTAGAAGCAGGGAAGATTTACTGGAAATCGGAAACCAGCTCACGCCAGCTGAGGCAAGGAGCTTTGCCGTAGCTCAACTCCCCAGGGAGGGATCAAAACACACTGGATTTGCTTTTGACTCGCCTGGATACGAATCATTTTTCGCTTCCCAGCTGGGAGTCCATGCCCCTCAAAAAGCATGGGATGTCGCTCGGAGAGCCAGCATGAAAAGGACACCGAAAAAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022622 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0048364 GO:0048367 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0097708 GO:0098791 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1225

Amino Acids

139.45

Weight (kDa)

8.67

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	46 - 110	8.1e-25	Phospholipid-translocating ATPase N-terminal
E1-E2_ATPase	PF00122	141 - 202	4.1e-06	P-type ATPase actuator domain
Cation_ATPase	PF13246	506 - 704	2e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	887 - 1133	9.7e-83	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59820
fragaria_vesca	FvH4_4g35440 FvH4_4g35440 FvH4_4g35440 FvH4_4g35440
malus_domestica	MD13G1083400.v1.1 MD16G1082100.v1.1
prunus_persica	Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1
pyrus_communis	pycom13g07180 pycom16g07110
rosa_chinensis	RchiOBHm_Chr4g0445071
rosa_laevigata	RLG00000005770
rosa_multiflora	Rmu_sc0003784.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385610
rosa_rugosa	Rorug04G0358500
rosa_samantha	Rh4AG419600 Rh4BG431300 Rh4CG445800
rosa_wichuraiana	Rw4G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2921
AatII	GACGTC	1 cut(s)	77
Acc36I	ACCTGC	1 cut(s)	472
AccB1I	GGYRCC	4 cut(s)	118, 334, 2349, 2585
AccBSI	CCGCTC	2 cut(s)	70, 1393
AccI	GTMKAC	2 cut(s)	77, 1169
AccII	CGCG	1 cut(s)	153
AccIII	TCCGGA	1 cut(s)	3089
AciI	CCGC	4 cut(s)	70, 130, 253, 1393
AclWI	GGATC	9 cut(s)	509, 607, 1460, 1924, 2028, 2599, 3373, 3386, 3544
AcoI	YGGCCR	1 cut(s)	3003
AcuI	CTGAAG	1 cut(s)	1513
AcvI	CACGTG	1 cut(s)	2312
AcyI	GRCGYC	2 cut(s)	74, 119
AdeI	CACNNNGTG	2 cut(s)	1555, 3192
AfeI	AGCGCT	1 cut(s)	395
AfiI	CCNNNNNNNGG	8 cut(s)	115, 1069, 1228, 1447, 1801, 2016, 3532, 3533
AflIII	ACRYGT	1 cut(s)	2311
AjnI	CCWGG	4 cut(s)	467, 1941, 3527, 3568
Alw21I	GWGCWC	1 cut(s)	3079
Alw26I	GTCTC	7 cut(s)	572, 654, 797, 1013, 2126, 2286, 2391
AlwI	GGATC	9 cut(s)	509, 607, 1460, 1924, 2028, 2599, 3373, 3386, 3544
AlwNI	CAGNNNCTG	1 cut(s)	2261
Ama87I	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
Aor13HI	TCCGGA	1 cut(s)	3089
Aor51HI	AGCGCT	1 cut(s)	395
AoxI	GGCC	3 cut(s)	1944, 3003, 3126
ArsI	GACNNNNNNTTYG	4 cut(s)	571, 603, 1228, 1260
Asp700I	GAANNNNTTC	4 cut(s)	395, 462, 2115, 2240
AspLEI	GCGC	3 cut(s)	121, 396, 2365
AspS9I	GGNCC	1 cut(s)	18
AsuC2I	CCSGG	5 cut(s)	123, 179, 180, 1013, 1580
AsuHPI	GGTGA	7 cut(s)	119, 1580, 1886, 2381, 2542, 2591, 2800
AsuII	TTCGAA	2 cut(s)	878, 1241
AvaI	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
AvaII	GGWCC	1 cut(s)	18
AxyI	CCTNAGG	1 cut(s)	1563
BamHI	GGATCC	1 cut(s)	3378
BanI	GGYRCC	4 cut(s)	118, 334, 2349, 2585
BbrPI	CACGTG	1 cut(s)	2312
BbsI	GAAGAC	1 cut(s)	2103
Bbv12I	GWGCWC	1 cut(s)	3079
BbvCI	CCTCAGC	1 cut(s)	3495
BccI	CCATC	9 cut(s)	3, 554, 1309, 1799, 1859, 2176, 2408, 2576, 3381
BceAI	ACGGC	3 cut(s)	1779, 2990, 3497
BciT130I	CCWGG	4 cut(s)	469, 1943, 3529, 3570
BciVI	GTATCC	1 cut(s)	3566
BclI	TGATCA	2 cut(s)	1152, 2220
BcnI	CCSGG	5 cut(s)	123, 179, 180, 1013, 1580
BcoDI	GTCTC	7 cut(s)	572, 654, 797, 1013, 2126, 2286, 2391
BfaI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
BfmI	CTRYAG	2 cut(s)	525, 1948
BfoI	RGCGCY	2 cut(s)	122, 397
BfuAI	ACCTGC	1 cut(s)	472
BfuI	GTATCC	1 cut(s)	3566
BglII	AGATCT	2 cut(s)	529, 2065
BmcAI	AGTACT	1 cut(s)	995
Bme1390I	CCNGG	9 cut(s)	123, 179, 180, 469, 1013, 1580, 1943, 3529, 3570
Bme18I	GGWCC	1 cut(s)	18
BmeT110I	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
BmgT120I	GGNCC	1 cut(s)	18
BmiI	GGNNCC	5 cut(s)	120, 336, 2351, 2587, 3380
BmrFI	CCNGG	9 cut(s)	123, 179, 180, 469, 1013, 1580, 1943, 3529, 3570
BmrI	ACTGGG	2 cut(s)	2680, 3335
BmsI	GCATC	5 cut(s)	325, 671, 1523, 1613, 2072
BmuI	ACTGGG	2 cut(s)	2680, 3335
BoxI	GACNNNNGTC	1 cut(s)	2728
BpiI	GAAGAC	1 cut(s)	2103
BpmI	CTGGAG	1 cut(s)	914
Bpu10I	CCTNAGC	2 cut(s)	1470, 3495
Bpu14I	TTCGAA	2 cut(s)	878, 1241
BpuEI	CTTGAG	2 cut(s)	2375, 2491
BpuMI	CCSGG	5 cut(s)	123, 179, 180, 1013, 1580
BsaAI	YACGTR	2 cut(s)	1931, 2312
BsaBI	GATNNNNATC	1 cut(s)	3577
BsaHI	GRCGYC	2 cut(s)	74, 119
BsaI	GGTCTC	2 cut(s)	1013, 2286
BsaJI	CCNNGG	9 cut(s)	178, 179, 467, 846, 1012, 1578, 3373, 3527, 3528
BsaWI	WCCGGW	1 cut(s)	3089
BsaXI	ACNNNNNCTCC	6 cut(s)	480, 510, 1332, 1362, 2283, 2313
Bsc4I	CCNNNNNNNGG	8 cut(s)	115, 1069, 1228, 1447, 1801, 2016, 3532, 3533
Bse118I	RCCGGY	1 cut(s)	1028
Bse21I	CCTNAGG	1 cut(s)	1563
Bse3DI	GCAATG	2 cut(s)	1438, 3219
Bse8I	GATNNNNATC	1 cut(s)	3577
BseAI	TCCGGA	1 cut(s)	3089
BseBI	CCWGG	4 cut(s)	469, 1943, 3529, 3570
BseDI	CCNNGG	9 cut(s)	178, 179, 467, 846, 1012, 1578, 3373, 3527, 3528
BseJI	GATNNNNATC	1 cut(s)	3577
BseLI	CCNNNNNNNGG	8 cut(s)	115, 1069, 1228, 1447, 1801, 2016, 3532, 3533
BseMI	GCAATG	2 cut(s)	1438, 3219
BseMII	CTCAG	8 cut(s)	332, 645, 808, 1554, 1695, 2502, 2561, 3486
BseRI	GAGGAG	2 cut(s)	42, 1497
BseYI	CCCAGC	3 cut(s)	37, 3594, 3598
BsgI	GTGCAG	2 cut(s)	704, 2949
Bsh1236I	CGCG	1 cut(s)	153
Bsh1285I	CGRYCG	1 cut(s)	151
BshFI	GGCC	3 cut(s)	1946, 3005, 3128
BshNI	GGYRCC	4 cut(s)	118, 334, 2349, 2585
BsiEI	CGRYCG	1 cut(s)	151
BsiHKAI	GWGCWC	1 cut(s)	3079
BsiHKCI	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
BsiSI	CCGG	7 cut(s)	122, 179, 1012, 1029, 1580, 3090, 3440
BslFI	GGGAC	3 cut(s)	1561, 2189, 3323
BslI	CCNNNNNNNGG	8 cut(s)	115, 1069, 1228, 1447, 1801, 2016, 3532, 3533
BsmAI	GTCTC	7 cut(s)	572, 654, 797, 1013, 2126, 2286, 2391
BsmBI	CGTCTC	1 cut(s)	654
BsmFI	GGGAC	3 cut(s)	1561, 2189, 3323
BsmI	GAATGC	3 cut(s)	865, 1030, 2655
BsnI	GGCC	3 cut(s)	1946, 3005, 3128
Bso31I	GGTCTC	2 cut(s)	1013, 2286
BsoBI	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
Bsp119I	TTCGAA	2 cut(s)	878, 1241
Bsp1286I	GDGCHC	1 cut(s)	3079
Bsp13I	TCCGGA	1 cut(s)	3089
Bsp1407I	TGTACA	1 cut(s)	711
Bsp68I	TCGCGA	1 cut(s)	153
BspACI	CCGC	4 cut(s)	70, 130, 253, 1393
BspANI	GGCC	3 cut(s)	1946, 3005, 3128
BspCNI	CTCAG	8 cut(s)	333, 646, 807, 1555, 1696, 2501, 2562, 3487
BspEI	TCCGGA	1 cut(s)	3089
BspFNI	CGCG	1 cut(s)	153
BspHI	TCATGA	1 cut(s)	838
BspLI	GGNNCC	5 cut(s)	120, 336, 2351, 2587, 3380
BspMAI	CTGCAG	1 cut(s)	529
BspMI	ACCTGC	1 cut(s)	472
BspPI	GGATC	9 cut(s)	509, 607, 1460, 1924, 2028, 2599, 3373, 3386, 3544
BspT104I	TTCGAA	2 cut(s)	878, 1241
BspT107I	GGYRCC	4 cut(s)	118, 334, 2349, 2585
BspTNI	GGTCTC	2 cut(s)	1013, 2286
BsrBI	CCGCTC	2 cut(s)	70, 1393
BsrDI	GCAATG	2 cut(s)	1438, 3219
BsrFI	RCCGGY	1 cut(s)	1028
BsrGI	TGTACA	1 cut(s)	711
BssAI	RCCGGY	1 cut(s)	1028
BssECI	CCNNGG	9 cut(s)	178, 179, 467, 846, 1012, 1578, 3373, 3527, 3528
BssNI	GRCGYC	2 cut(s)	74, 119
BssT1I	CCWWGG	2 cut(s)	846, 3373
Bst2UI	CCWGG	4 cut(s)	469, 1943, 3529, 3570
Bst4CI	ACNGT	9 cut(s)	91, 246, 823, 1064, 1555, 2241, 2381, 2727, 3151
BstACI	GRCGYC	2 cut(s)	74, 119
BstAPI	GCANNNNNTGC	1 cut(s)	929
BstAUI	TGTACA	1 cut(s)	711
BstBAI	YACGTR	2 cut(s)	1931, 2312
BstBI	TTCGAA	2 cut(s)	878, 1241
BstC8I	GCNNGC	6 cut(s)	1030, 1811, 2212, 2655, 3492, 3646
BstEII	GGTNACC	1 cut(s)	125
BstENI	CCTNNNNNAGG	1 cut(s)	2014
BstFNI	CGCG	1 cut(s)	153
BstH2I	RGCGCY	2 cut(s)	122, 397
BstHHI	GCGC	3 cut(s)	121, 396, 2365
BstMAI	GTCTC	7 cut(s)	572, 654, 797, 1013, 2126, 2286, 2391
BstMCI	CGRYCG	1 cut(s)	151
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 118, 150, 929, 2663, 3641
BstNI	CCWGG	4 cut(s)	469, 1943, 3529, 3570
BstNSI	RCATGY	1 cut(s)	2214
BstPAI	GACNNNNGTC	1 cut(s)	2728
BstPI	GGTNACC	1 cut(s)	125
BstSCI	CCNGG	9 cut(s)	121, 177, 178, 467, 1011, 1578, 1941, 3527, 3568
BstSFI	CTRYAG	2 cut(s)	525, 1948
BstSNI	TACGTA	1 cut(s)	1931
BstUI	CGCG	1 cut(s)	153
BstV2I	GAAGAC	1 cut(s)	2103
BstX2I	RGATCY	5 cut(s)	529, 612, 1916, 2065, 3378
BstYI	RGATCY	5 cut(s)	529, 612, 1916, 2065, 3378
Bsu36I	CCTNAGG	1 cut(s)	1563
BsuI	GTATCC	1 cut(s)	3566
BsuRI	GGCC	3 cut(s)	1946, 3005, 3128
BtsI	GCAGTG	3 cut(s)	692, 976, 2085
BtuMI	TCGCGA	1 cut(s)	153
BveI	ACCTGC	1 cut(s)	472
Cac8I	GCNNGC	6 cut(s)	1030, 1811, 2212, 2655, 3492, 3646
CaiI	CAGNNNCTG	1 cut(s)	2261
CciI	TCATGA	1 cut(s)	838
CfoI	GCGC	3 cut(s)	121, 396, 2365
Cfr10I	RCCGGY	1 cut(s)	1028
Cfr13I	GGNCC	1 cut(s)	18
Cfr9I	CCCGGG	1 cut(s)	178
CseI	GACGC	2 cut(s)	1766, 2467
DinI	GGCGCC	1 cut(s)	120
DraIII	CACNNNGTG	2 cut(s)	1555, 3192
DrdI	GACNNNNNNGTC	1 cut(s)	2921
DseDI	GACNNNNNNGTC	1 cut(s)	2921
EaeI	YGGCCR	1 cut(s)	3003
Eco105I	TACGTA	1 cut(s)	1931
Eco130I	CCWWGG	2 cut(s)	846, 3373
Eco31I	GGTCTC	2 cut(s)	1013, 2286
Eco47I	GGWCC	1 cut(s)	18
Eco47III	AGCGCT	1 cut(s)	395
Eco57I	CTGAAG	1 cut(s)	1513
Eco72I	CACGTG	1 cut(s)	2312
Eco81I	CCTNAGG	1 cut(s)	1563
Eco88I	CYCGRG	5 cut(s)	22, 65, 98, 178, 3064
Eco91I	GGTNACC	1 cut(s)	125
EcoNI	CCTNNNNNAGG	1 cut(s)	2014
EcoO65I	GGTNACC	1 cut(s)	125
EcoRI	GAATTC	3 cut(s)	184, 668, 1523
EcoRII	CCWGG	4 cut(s)	467, 1941, 3527, 3568
EcoT14I	CCWWGG	2 cut(s)	846, 3373
EcoT22I	ATGCAT	2 cut(s)	2212, 2983
EgeI	GGCGCC	1 cut(s)	120
EheI	GGCGCC	1 cut(s)	120
ErhI	CCWWGG	2 cut(s)	846, 3373
Esp3I	CGTCTC	1 cut(s)	654
FalI	AAGNNNNNCTT	6 cut(s)	194, 226, 1511, 1543, 2088, 2120
FaqI	GGGAC	3 cut(s)	1561, 2189, 3323
FauI	CCCGC	1 cut(s)	246
FauNDI	CATATG	1 cut(s)	1972
FbaI	TGATCA	2 cut(s)	1152, 2220
FblI	GTMKAC	2 cut(s)	77, 1169
FspBI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
GlaI	GCGC	3 cut(s)	120, 395, 2364
GsaI	CCCAGC	3 cut(s)	41, 3598, 3602
GsuI	CTGGAG	1 cut(s)	914
HaeII	RGCGCY	2 cut(s)	122, 397
HaeIII	GGCC	3 cut(s)	1946, 3005, 3128
HapII	CCGG	7 cut(s)	122, 179, 1012, 1029, 1580, 3090, 3440
HgaI	GACGC	2 cut(s)	1766, 2467
HhaI	GCGC	3 cut(s)	121, 396, 2365
Hin1I	GRCGYC	2 cut(s)	74, 119
Hin6I	GCGC	3 cut(s)	119, 394, 2363
HinP1I	GCGC	3 cut(s)	119, 394, 2363
HincII	GTYRAC	3 cut(s)	78, 2793, 3154
HindII	GTYRAC	3 cut(s)	78, 2793, 3154
HindIII	AAGCTT	2 cut(s)	1472, 2102
HpaII	CCGG	7 cut(s)	122, 179, 1012, 1029, 1580, 3090, 3440
HphI	GGTGA	7 cut(s)	119, 1580, 1886, 2381, 2542, 2591, 2800
Hpy99I	CGWCG	3 cut(s)	79, 82, 2906
HpyAV	CCTTC	8 cut(s)	727, 885, 1088, 1441, 2105, 2639, 3319, 3437
HpyCH4III	ACNGT	9 cut(s)	91, 246, 823, 1064, 1555, 2241, 2381, 2727, 3151
HpyCH4IV	ACGT	8 cut(s)	74, 749, 1666, 1930, 2311, 2789, 3285, 3294
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 118, 150, 929, 2663, 3641
HpySE526I	ACGT	8 cut(s)	74, 749, 1666, 1930, 2311, 2789, 3285, 3294
Hsp92I	GRCGYC	2 cut(s)	74, 119
HspAI	GCGC	3 cut(s)	119, 394, 2363
KasI	GGCGCC	1 cut(s)	118
Kpn2I	TCCGGA	1 cut(s)	3089
KroI	GCCGGC	1 cut(s)	1028
KroNI	GCCGGC	1 cut(s)	1030
Ksp22I	TGATCA	2 cut(s)	1152, 2220
LmnI	GCTCC	3 cut(s)	29, 959, 3504
LweI	GCATC	5 cut(s)	325, 671, 1523, 1613, 2072
MaeI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
MaeII	ACGT	8 cut(s)	74, 749, 1666, 1930, 2311, 2789, 3285, 3294
MbiI	CCGCTC	2 cut(s)	70, 1393
MfeI	CAATTG	1 cut(s)	1335
MflI	RGATCY	5 cut(s)	529, 612, 1916, 2065, 3378
MhlI	GDGCHC	1 cut(s)	3079
Mly113I	GGCGCC	1 cut(s)	119
MlyI	GAGTC	9 cut(s)	76, 114, 228, 1583, 1625, 2128, 2788, 3557, 3612
MmeI	TCCRAC	5 cut(s)	466, 1469, 2016, 2608, 3190
Mph1103I	ATGCAT	2 cut(s)	2212, 2983
MroI	TCCGGA	1 cut(s)	3089
MroNI	GCCGGC	1 cut(s)	1028
MroXI	GAANNNNTTC	4 cut(s)	395, 462, 2115, 2240
MseI	TTAA	7 cut(s)	369, 1326, 1455, 1761, 1958, 2154, 3038
MslI	CAYNNNNRTG	2 cut(s)	1148, 3432
MspA1I	CMGCKG	2 cut(s)	3494, 3598
MspI	CCGG	7 cut(s)	122, 179, 1012, 1029, 1580, 3090, 3440
MspR9I	CCNGG	9 cut(s)	123, 179, 180, 469, 1013, 1580, 1943, 3529, 3570
MunI	CAATTG	1 cut(s)	1335
Mva1269I	GAATGC	3 cut(s)	865, 1030, 2655
MvaI	CCWGG	4 cut(s)	469, 1943, 3529, 3570
MvnI	CGCG	1 cut(s)	153
MwoI	GCNNNNNNNGC	6 cut(s)	67, 118, 150, 929, 2663, 3641
NaeI	GCCGGC	1 cut(s)	1030
NarI	GGCGCC	1 cut(s)	119
NciI	CCSGG	5 cut(s)	123, 179, 180, 1013, 1580
NdeI	CATATG	1 cut(s)	1972
NgoMIV	GCCGGC	1 cut(s)	1028
NlaIV	GGNNCC	5 cut(s)	120, 336, 2351, 2587, 3380
NmuCI	GTSAC	6 cut(s)	125, 1547, 2455, 2530, 3055, 3145
NruI	TCGCGA	1 cut(s)	153
NsiI	ATGCAT	2 cut(s)	2212, 2983
NspI	RCATGY	1 cut(s)	2214
NspV	TTCGAA	2 cut(s)	878, 1241
PaeI	GCATGC	1 cut(s)	2214
PaeR7I	CTCGAG	2 cut(s)	22, 3064
PagI	TCATGA	1 cut(s)	838
PasI	CCCWGGG	1 cut(s)	3528
PcsI	WCGNNNNNNNCGW	2 cut(s)	29, 3291
PctI	GAATGC	3 cut(s)	865, 1030, 2655
PdiI	GCCGGC	1 cut(s)	1030
PdmI	GAANNNNTTC	4 cut(s)	395, 462, 2115, 2240
PfeI	GAWTC	7 cut(s)	13, 174, 1694, 1741, 2776, 2838, 3578
Ple19I	CGATCG	1 cut(s)	151
PleI	GAGTC	9 cut(s)	76, 113, 228, 1582, 1624, 2128, 2788, 3557, 3611
PluTI	GGCGCC	1 cut(s)	122
PmaCI	CACGTG	1 cut(s)	2312
PmlI	CACGTG	1 cut(s)	2312
PpsI	GAGTC	9 cut(s)	76, 113, 228, 1582, 1624, 2128, 2788, 3557, 3611
Ppu21I	YACGTR	2 cut(s)	1931, 2312
PshAI	GACNNNNGTC	1 cut(s)	2728
Psp6I	CCWGG	4 cut(s)	467, 1941, 3527, 3568
PspCI	CACGTG	1 cut(s)	2312
PspEI	GGTNACC	1 cut(s)	125
PspFI	CCCAGC	3 cut(s)	37, 3594, 3598
PspGI	CCWGG	4 cut(s)	467, 1941, 3527, 3568
PspN4I	GGNNCC	5 cut(s)	120, 336, 2351, 2587, 3380
PspPI	GGNCC	1 cut(s)	18
PspXI	VCTCGAGB	1 cut(s)	3064
PstI	CTGCAG	1 cut(s)	529
PstNI	CAGNNNCTG	1 cut(s)	2261
PsuI	RGATCY	5 cut(s)	529, 612, 1916, 2065, 3378
PvuI	CGATCG	1 cut(s)	151
PvuII	CAGCTG	2 cut(s)	3494, 3598
RruI	TCGCGA	1 cut(s)	153
RseI	CAYNNNNRTG	2 cut(s)	1148, 3432
SalI	GTCGAC	1 cut(s)	76
SaqAI	TTAA	7 cut(s)	369, 1326, 1455, 1761, 1958, 2154, 3038
Sau96I	GGNCC	1 cut(s)	18
ScaI	AGTACT	1 cut(s)	995
SchI	GAGTC	9 cut(s)	76, 114, 228, 1583, 1625, 2128, 2788, 3557, 3612
ScrFI	CCNGG	9 cut(s)	123, 179, 180, 469, 1013, 1580, 1943, 3529, 3570
SduI	GDGCHC	1 cut(s)	3079
SfaNI	GCATC	5 cut(s)	325, 671, 1523, 1613, 2072
SfcI	CTRYAG	2 cut(s)	525, 1948
SfoI	GGCGCC	1 cut(s)	120
Sfr274I	CTCGAG	2 cut(s)	22, 3064
SfuI	TTCGAA	2 cut(s)	878, 1241
SgrDI	CGTCGACG	1 cut(s)	76
SinI	GGWCC	1 cut(s)	18
SlaI	CTCGAG	2 cut(s)	22, 3064
SmaI	CCCGGG	1 cut(s)	180
SmiMI	CAYNNNNRTG	2 cut(s)	1148, 3432
SmlI	CTYRAG	4 cut(s)	22, 2354, 2470, 3064
SmoI	CTYRAG	4 cut(s)	22, 2354, 2470, 3064
SnaBI	TACGTA	1 cut(s)	1931
SphI	GCATGC	1 cut(s)	2214
SsiI	CCGC	4 cut(s)	70, 130, 253, 1393
SspDI	GGCGCC	1 cut(s)	118
SspI	AATATT	1 cut(s)	163
SspMI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
StyD4I	CCNGG	9 cut(s)	121, 177, 178, 467, 1011, 1578, 1941, 3527, 3568
StyI	CCWWGG	2 cut(s)	846, 3373
TaaI	ACNGT	9 cut(s)	91, 246, 823, 1064, 1555, 2241, 2381, 2727, 3151
TaiI	ACGT	8 cut(s)	77, 752, 1669, 1933, 2314, 2792, 3288, 3297
TaqI	TCGA	9 cut(s)	23, 53, 77, 878, 1209, 1241, 2503, 3065, 3402
TaqII	GACCGA	1 cut(s)	6
TatI	WGTACW	8 cut(s)	204, 267, 711, 887, 993, 1107, 1750, 1821
TfiI	GAWTC	7 cut(s)	13, 174, 1694, 1741, 2776, 2838, 3578
Tru1I	TTAA	7 cut(s)	369, 1326, 1455, 1761, 1958, 2154, 3038
Tru9I	TTAA	7 cut(s)	369, 1326, 1455, 1761, 1958, 2154, 3038
TseFI	GTSAC	6 cut(s)	125, 1547, 2455, 2530, 3055, 3145
Tsp45I	GTSAC	6 cut(s)	125, 1547, 2455, 2530, 3055, 3145
TspGWI	ACGGA	2 cut(s)	1817, 2681
TspMI	CCCGGG	1 cut(s)	178
VpaK11BI	GGWCC	1 cut(s)	18
XagI	CCTNNNNNAGG	1 cut(s)	2014
XceI	RCATGY	1 cut(s)	2214
XhoI	CTCGAG	2 cut(s)	22, 3064
XmaI	CCCGGG	1 cut(s)	178
XmiI	GTMKAC	2 cut(s)	77, 1169
XmnI	GAANNNNTTC	4 cut(s)	395, 462, 2115, 2240
XspI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
ZraI	GACGTC	1 cut(s)	75
ZrmI	AGTACT	1 cut(s)	995
Zsp2I	ATGCAT	2 cut(s)	2212, 2983

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.