Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010275

Physical Location & Seq

Genomic Coordinates Forward (+)

5832088 .. 5850847

Gene Structure 18760 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1083400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3675 bp

ATGAGTGGATGGAATCGGTCCTCGAGAGGAGCGAGGCTGGGCGGTGGTCGCAATTCGAGTTTGCCGCCCGGGAGCACGTCGACGGTTCGACTCGGAAGAGTCCAGCCACAGGCGCCGGGTCACCGCACGATTTTCTGCAACGATCGCGAGCATAACATTCCCGTCAGATTCCCGGGGAATTCTATATCGACTACAAAGTACAACTTCATTACATTTTTGCCGAAAGGACTCTTTGAACAGTTCAGGCGGGTAGCTAACCTCTACTTTCTGACGATCTCAATTCTATCAACAACGCCAATCAGTCCTGTGCATCCTGTAACCAATGTGGTCCCTCTGAGTTTGGTGCTTTTTGTTTCTCTTGTTAAGGAAGCATTTGAGGATTGGAAGCGCTTCCAGAATGATATGACCATAAATAATGCTTCTGTTGATGTCTTGCAAGATCAGAAATGGGAAAGTATTCCCTGGAAAAAGTTGCAGGTTGGAGATATTGTCAGGATTAAGCAGAACGGATATTTCCCTGCAGATCTGCTTTTTCTTGCTGGTACAAACCCAGATGGTGTCTGCTACATTGAGACTGCAAATTTGGATGGGGAAACTAATTTGAAGATCAGAAAAGCATTAGAAAAGACTTGGGATTACTCGACTCCTGACAAAGCATCTGAATTCAAAGGAGAAGTGCAGTGTGAACAACCTAACAATTCATTGTACACCTACACTGGAAATCTCATTATTGACAAGCAGACATTACCTCTATCTCCAAACCAAATTCTGCTGCGAGGTTGCAGTCTCAGGAATACTGAGTACATTGTTGCGACTGTTATATTTACAGGTCATGAAACCAAGGTTATGATGAATGCCATGAATGTTCCTTCGAAAAGAAGTACACTGGAGAGGAAACTTGACAAACTTATACTCGCGCTTTTTGCTTTTCTCTTTATGTTGTGTTTGATTGGAGCTATCGGCAGTGGTGTATTCATCAACCGCAAGTACTACTACTTAGGTCTCTGGGGAAAAAAGAGCGGCGACTCAGCAATTGCATACAGCTCGTTTGACCCTAATAACCGTTTTCTGGTTATCATTCTGACGATGTTTACCCTCATTACTCTGTACTCAACAATAATCCCTATTTCTCTTTATGTTTCCATTGAGATGATCAAATTTATCCAGTCTACCCAATATATCAATAATGACTTGCGTATGTACCATGTCGAAAGCAATACCCCTGCCTTGGCTAGGACGTCAAATTTGAATGAAGAACTTGGACAGGTGGAATACATTTTTTCTGATAAAACTGGAACCCTAACAAGAAACTTGATGGAGTTCTTTAAGTGTTCAATTGGAGGGGAGGTCTATGGAACTGGTATCACTGAAATTGAAAGGGGAATAGCAGAGCGGAATGGTATAAAACTTGTTGAGGGTTACAATTCAGGCAACGCAGCCCATGAGAAGGGATTCAACTTTGATGATCCTAAGCTTATGCGAGGAGGTTGGAGGAATGAGCCTAATCCTGATCTTTGTAAGGAATTCTTCAGATGCCTTGCTATATGTCACACAGTGCTTCCTGAGGGTGAAGAGTCCCCAGAAAAAATCACATATCAAGCTGCATCCCCTGATGAGTCTGCTTTGGTAATTGCTGCAAAGAACTTTGGCTTCTTCTTTCACAGACGTACACCGACGACGATATATATTCGTGAATCTCACACTGAGAAAATCGGTACAGTTCAAGGTGTGTCTTACGAGATTCTCAATGTGCTTGAATTTAATAGTACAAGGAAGCGTCAGTCTGTTGTATGCCGTTACACAGATGGCAGGCTTGTTTTGTACTGCAAGGGTGCTGATAATGTAATCTACGAGAGATTGGCTGATGGACAAGATGATTTGAAAAAAGTATCTAGGGAACACTTGGAATTATTTGGATCTGCTGGATTACGTACTCTTTGCTTGGCCTGTAGAGATTTAAGTCCTGATACATATGAAAGCTGGAATGAGAAATTTATTCAGGCTAAATCCTCACTACGGGACCGTGAAAAGAAGTTGGATGAGGTGGCAGAAATTATAGAGAAAGATCTCATTTTGATTGGATGCACTGCCATCGAAGATAAGCTTCAAGAAGGAGTCCCAAATTGCATAGAGACTCTATCAAGAGCTGGTATTAAGATTTGGGTGCTAACAGGGGACAAGATGGAAACTGCAATAAATATAGCTTATGCATGTAATTTGATCAACAATGAAATGAAACAGTTTATTATCAGTTCAGAAACTGATGTGATTAGGGAAGTTGAGGACAGGGGAGACCAAATGGAAATTGCACGTGTCATTAAAGAAGAAGTCAAAAAAGACCTAAAAAGGTGCCTCGAGGAAGCGCAAAACTATCTTCACAGTGTGTCTGCACCAAAACTAGCACTTGTAATAGATGGGAAGTGCTTGATGTATGCCTTAGACCCCAGTCTACGAGTGACGCTACTCAACTTGAGCTTGAATTGTAACTCAGTTGTTTGCTGTCGAGTTTCTCCTTTGCAGAAAGCACAGGTGACAAGTATGGTAAAAAAGGGTGCAAAGAAGATAACACTGAGCATTGGTGATGGTGCCAATGATGTGAGCATGATCCAAGCTGCTCATGTTGGCGTTGGAATAAGTGGGCAAGAAGGAATGCAAGCAGTGATGGCTAGTGATTTTGCTATTGCCCAGTTCCGCTTTCTTACTGATTTATTGCTTGTGCATGGACGGTGGTCATATATTAGATTATGCAAGGTTGTTACATACTTCTTTTACAAGAATCTTACATTCACGTTGACTCAATTTTGGTTCACCTTTCAAACTGGATTTTCTGGTCAAAGATTCTACGATGATTGGTTTCAGTCATTGTATAATGTCATATTCACAGCCTTGCCTGTAATCATCGTTGGGCTTTTTGACAAGGATGTCAGTGCAGCCCTCTCCAAGAAGTACCCTGAACTATACAGGGAGGGAATAAGAAATGCCTTTTTCAAATGGAGGGTTGTGGCAATATGGGCTTTCTTCTCCGTTTACCAATCTTTAATCTTCTATTATTTTGTAACTTCCTCAAGTGATCGTGGTCAAAATTCATCCGGAAAGATGTTTGGACTTTGGGATGTCAGCACAATGGCCTTTACTTGTGTTGTAGTGACCGTCAACTTTCGTTTGCTTATGATGTGCAATTCAATCACAAGGTGGCATTATATTAGTGTTGGAGGAAGCCTCGCAGCATGGTTTATATTCATCTTCATATATTGTATCATAGACCAGAAGAGAAATCTTTATTACGTCATATATGTCCTGATGAGTACCTTCTATTTCTACCTGACACTTCTTCTAGTCCCAGTTTTTGCACTTTTTGGCGACTTCGTTTACCAAGGGATCCAGAGATGGTTCTTTCCTTTCGATTATCAGATAATTCAGGAAATTCACAGGCATGAGCCCGAAGGTAGAAGCAGGGATGATTTACTGGAAATCGGAAACCAGCTCACCCCAGCTGAGGCCAGGAGCTTTGCCGTAGCGCAACTCCCTCGGGAGGTATCAAAACACACAGGGTTTGCTTTTGACTCTCCTGGATACGAATCATTTTTTGCTTCCCAGCTGGGAGTCCATGCCCCTCAAAAAGCATGGGATGTCGCTCGGAGAGCCAGCTTGAAAAGAACAGCGAAAAAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0007275 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022622 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0048364 GO:0048367 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0097708 GO:0098791 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1225

Amino Acids

139.0

Weight (kDa)

8.57

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	44 - 108	3e-25	Phospholipid-translocating ATPase N-terminal
E1-E2_ATPase	PF00122	139 - 201	3.4e-06	P-type ATPase actuator domain
Cation_ATPase	PF13246	506 - 704	2.4e-20	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	887 - 1133	8.8e-83	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59820
fragaria_vesca	FvH4_4g35440 FvH4_4g35440 FvH4_4g35440 FvH4_4g35440
malus_domestica	MD13G1083400.v1.1 MD16G1082100.v1.1
prunus_persica	Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1 Prupe.1G263700_v2.0.a1
pyrus_communis	pycom13g07180 pycom16g07110
rosa_chinensis	RchiOBHm_Chr4g0445071
rosa_laevigata	RLG00000005770
rosa_multiflora	Rmu_sc0003784.1_g000007
rosa_roxburghii	Rroxscaffold_5G00385610
rosa_rugosa	Rorug04G0358500
rosa_samantha	Rh4AG419600 Rh4BG431300 Rh4CG445800
rosa_wichuraiana	Rw4G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2921
AatII	GACGTC	1 cut(s)	1241
AbsI	CCTCGAGG	1 cut(s)	2354
Acc36I	ACCTGC	1 cut(s)	466
AccB1I	GGYRCC	3 cut(s)	112, 2349, 2585
AccBSI	CCGCTC	2 cut(s)	1020, 1393
AccI	GTMKAC	3 cut(s)	80, 1169, 2449
AccII	CGCG	2 cut(s)	147, 917
AccIII	TCCGGA	1 cut(s)	3089
AciI	CCGC	8 cut(s)	42, 65, 124, 247, 982, 1020, 1393, 2692
AclWI	GGATC	5 cut(s)	1460, 1924, 2599, 3373, 3386
AcuI	CTGAAG	1 cut(s)	1513
AcvI	CACGTG	1 cut(s)	2312
AcyI	GRCGYC	2 cut(s)	113, 1238
AdeI	CACNNNGTG	3 cut(s)	1555, 2383, 3192
AfeI	AGCGCT	1 cut(s)	389
AfiI	CCNNNNNNNGG	8 cut(s)	109, 1069, 1228, 1233, 1447, 2016, 3496, 3532
AflIII	ACRYGT	1 cut(s)	2311
AjiI	CACGTC	1 cut(s)	78
AjnI	CCWGG	3 cut(s)	461, 3500, 3568
Alw21I	GWGCWC	1 cut(s)	77
Alw26I	GTCTC	5 cut(s)	566, 791, 1007, 2126, 2286
AlwI	GGATC	5 cut(s)	1460, 1924, 2599, 3373, 3386
AlwNI	CAGNNNCTG	1 cut(s)	2261
Ama87I	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
Aor13HI	TCCGGA	1 cut(s)	3089
Aor51HI	AGCGCT	1 cut(s)	389
AoxI	GGCC	3 cut(s)	1944, 3126, 3498
ApeKI	GCWGC	7 cut(s)	772, 1436, 1601, 1634, 2612, 2930, 3224
ArsI	GACNNNNNNTTYG	4 cut(s)	565, 597, 1228, 1260
Asp700I	GAANNNNTTC	3 cut(s)	389, 456, 1451
AspLEI	GCGC	5 cut(s)	115, 390, 919, 2365, 3520
AspS9I	GGNCC	3 cut(s)	18, 328, 2020
AsuC2I	CCSGG	5 cut(s)	69, 70, 117, 173, 174
AsuHPI	GGTGA	6 cut(s)	113, 1580, 2542, 2591, 2800, 3478
AsuII	TTCGAA	1 cut(s)	872
AvaI	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
AvaII	GGWCC	3 cut(s)	18, 328, 2020
AxyI	CCTNAGG	1 cut(s)	1563
BamHI	GGATCC	1 cut(s)	3378
BanI	GGYRCC	3 cut(s)	112, 2349, 2585
BanII	GRGCYC	1 cut(s)	3441
BbrPI	CACGTG	1 cut(s)	2312
Bbv12I	GWGCWC	1 cut(s)	77
BbvCI	CCTCAGC	1 cut(s)	3495
BbvI	GCAGC	7 cut(s)	759, 1448, 1588, 1621, 2599, 2942, 3236
BceAI	ACGGC	2 cut(s)	1779, 3497
BcgI	CGANNNNNNTGC	6 cut(s)	1026, 1060, 2074, 2108, 3509, 3543
BciT130I	CCWGG	3 cut(s)	463, 3502, 3570
BciVI	GTATCC	1 cut(s)	3566
BclI	TGATCA	2 cut(s)	1152, 2220
BcnI	CCSGG	5 cut(s)	69, 70, 117, 173, 174
BcoDI	GTCTC	5 cut(s)	566, 791, 1007, 2126, 2286
BfaI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
BfmI	CTRYAG	2 cut(s)	519, 1948
BfoI	RGCGCY	2 cut(s)	116, 391
BfuAI	ACCTGC	1 cut(s)	466
BfuI	GTATCC	1 cut(s)	3566
BglII	AGATCT	2 cut(s)	523, 2065
BisI	GCNGC	9 cut(s)	65, 773, 1021, 1437, 1602, 1635, 2613, 2931, 3225
BlsI	GCNGC	9 cut(s)	66, 774, 1022, 1438, 1603, 1636, 2614, 2932, 3226
BmcAI	AGTACT	1 cut(s)	989
Bme1390I	CCNGG	8 cut(s)	69, 70, 117, 173, 174, 463, 3502, 3570
Bme18I	GGWCC	3 cut(s)	18, 328, 2020
BmeT110I	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
BmgBI	CACGTC	1 cut(s)	78
BmgT120I	GGNCC	3 cut(s)	18, 328, 2020
BmiI	GGNNCC	7 cut(s)	114, 330, 1297, 2021, 2351, 2587, 3380
BmrFI	CCNGG	8 cut(s)	69, 70, 117, 173, 174, 463, 3502, 3570
BmrI	ACTGGG	3 cut(s)	2439, 2680, 3335
BmsI	GCATC	5 cut(s)	319, 665, 1523, 1613, 2072
BmuI	ACTGGG	3 cut(s)	2439, 2680, 3335
BoxI	GACNNNNGTC	2 cut(s)	2445, 2728
BplI	GAGNNNNNCTC	2 cut(s)	3204, 3236
BpmI	CTGGAG	1 cut(s)	908
Bpu10I	CCTNAGC	2 cut(s)	1470, 3495
Bpu14I	TTCGAA	1 cut(s)	872
BpuEI	CTTGAG	2 cut(s)	2491, 3049
BpuMI	CCSGG	5 cut(s)	69, 70, 117, 173, 174
BsaAI	YACGTR	2 cut(s)	1931, 2312
BsaBI	GATNNNNATC	1 cut(s)	3577
BsaHI	GRCGYC	2 cut(s)	113, 1238
BsaI	GGTCTC	2 cut(s)	1007, 2286
BsaJI	CCNNGG	8 cut(s)	68, 172, 173, 461, 840, 1227, 3373, 3527
BsaWI	WCCGGW	1 cut(s)	3089
BsaXI	ACNNNNNCTCC	4 cut(s)	474, 504, 1332, 1362
Bsc4I	CCNNNNNNNGG	8 cut(s)	109, 1069, 1228, 1233, 1447, 2016, 3496, 3532
Bse21I	CCTNAGG	1 cut(s)	1563
Bse8I	GATNNNNATC	1 cut(s)	3577
BseAI	TCCGGA	1 cut(s)	3089
BseBI	CCWGG	3 cut(s)	463, 3502, 3570
BseDI	CCNNGG	8 cut(s)	68, 172, 173, 461, 840, 1227, 3373, 3527
BseJI	GATNNNNATC	1 cut(s)	3577
BseLI	CCNNNNNNNGG	8 cut(s)	109, 1069, 1228, 1233, 1447, 2016, 3496, 3532
BseMII	CTCAG	9 cut(s)	326, 789, 802, 1041, 1554, 1695, 2502, 2561, 3486
BseRI	GAGGAG	2 cut(s)	42, 1497
BseXI	GCAGC	7 cut(s)	759, 1448, 1588, 1621, 2599, 2942, 3236
BseYI	CCCAGC	4 cut(s)	37, 3490, 3594, 3598
BsgI	GTGCAG	3 cut(s)	698, 2373, 2949
Bsh1236I	CGCG	2 cut(s)	147, 917
Bsh1285I	CGRYCG	1 cut(s)	145
BshFI	GGCC	3 cut(s)	1946, 3128, 3500
BshNI	GGYRCC	3 cut(s)	112, 2349, 2585
BsiEI	CGRYCG	1 cut(s)	145
BsiHKAI	GWGCWC	1 cut(s)	77
BsiHKCI	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
BsiSI	CCGG	4 cut(s)	69, 116, 173, 3090
BslFI	GGGAC	6 cut(s)	314, 1561, 2033, 2102, 2189, 3323
BslI	CCNNNNNNNGG	8 cut(s)	109, 1069, 1228, 1233, 1447, 2016, 3496, 3532
BsmAI	GTCTC	5 cut(s)	566, 791, 1007, 2126, 2286
BsmFI	GGGAC	6 cut(s)	314, 1561, 2033, 2102, 2189, 3323
BsmI	GAATGC	2 cut(s)	859, 2655
BsnI	GGCC	3 cut(s)	1946, 3128, 3500
Bso31I	GGTCTC	2 cut(s)	1007, 2286
BsoBI	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
Bsp119I	TTCGAA	1 cut(s)	872
Bsp1286I	GDGCHC	2 cut(s)	77, 3441
Bsp13I	TCCGGA	1 cut(s)	3089
Bsp1407I	TGTACA	1 cut(s)	705
Bsp68I	TCGCGA	1 cut(s)	147
BspACI	CCGC	8 cut(s)	42, 65, 124, 247, 982, 1020, 1393, 2692
BspANI	GGCC	3 cut(s)	1946, 3128, 3500
BspCNI	CTCAG	9 cut(s)	327, 790, 801, 1040, 1555, 1696, 2501, 2562, 3487
BspEI	TCCGGA	1 cut(s)	3089
BspFNI	CGCG	2 cut(s)	147, 917
BspHI	TCATGA	1 cut(s)	832
BspLI	GGNNCC	7 cut(s)	114, 330, 1297, 2021, 2351, 2587, 3380
BspMAI	CTGCAG	1 cut(s)	523
BspMI	ACCTGC	1 cut(s)	466
BspPI	GGATC	5 cut(s)	1460, 1924, 2599, 3373, 3386
BspT104I	TTCGAA	1 cut(s)	872
BspT107I	GGYRCC	3 cut(s)	112, 2349, 2585
BspTNI	GGTCTC	2 cut(s)	1007, 2286
BsrBI	CCGCTC	2 cut(s)	1020, 1393
BsrGI	TGTACA	1 cut(s)	705
BssECI	CCNNGG	8 cut(s)	68, 172, 173, 461, 840, 1227, 3373, 3527
BssNI	GRCGYC	2 cut(s)	113, 1238
BssT1I	CCWWGG	3 cut(s)	840, 1227, 3373
Bst2UI	CCWGG	3 cut(s)	463, 3502, 3570
Bst6I	CTCTTC	3 cut(s)	91, 1566, 3263
BstACI	GRCGYC	2 cut(s)	113, 1238
BstAUI	TGTACA	1 cut(s)	705
BstBAI	YACGTR	2 cut(s)	1931, 2312
BstBI	TTCGAA	1 cut(s)	872
BstC8I	GCNNGC	4 cut(s)	149, 1811, 2655, 3646
BstEII	GGTNACC	1 cut(s)	119
BstENI	CCTNNNNNAGG	1 cut(s)	1231
BstFNI	CGCG	2 cut(s)	147, 917
BstH2I	RGCGCY	2 cut(s)	116, 391
BstHHI	GCGC	5 cut(s)	115, 390, 919, 2365, 3520
BstMAI	GTCTC	5 cut(s)	566, 791, 1007, 2126, 2286
BstMCI	CGRYCG	1 cut(s)	145
BstMWI	GCNNNNNNNGC	7 cut(s)	48, 112, 144, 923, 2663, 3011, 3641
BstNI	CCWGG	3 cut(s)	463, 3502, 3570
BstNSI	RCATGY	1 cut(s)	2214
BstPAI	GACNNNNGTC	2 cut(s)	2445, 2728
BstPI	GGTNACC	1 cut(s)	119
BstSCI	CCNGG	8 cut(s)	67, 68, 115, 171, 172, 461, 3500, 3568
BstSFI	CTRYAG	2 cut(s)	519, 1948
BstSNI	TACGTA	1 cut(s)	1931
BstUI	CGCG	2 cut(s)	147, 917
BstV1I	GCAGC	7 cut(s)	759, 1448, 1588, 1621, 2599, 2942, 3236
BstX2I	RGATCY	4 cut(s)	523, 1916, 2065, 3378
BstYI	RGATCY	4 cut(s)	523, 1916, 2065, 3378
Bsu36I	CCTNAGG	1 cut(s)	1563
BsuI	GTATCC	1 cut(s)	3566
BsuRI	GGCC	3 cut(s)	1946, 3128, 3500
BtrI	CACGTC	1 cut(s)	78
BtsI	GCAGTG	4 cut(s)	686, 970, 2085, 2664
BtuMI	TCGCGA	1 cut(s)	147
BveI	ACCTGC	1 cut(s)	466
Cac8I	GCNNGC	4 cut(s)	149, 1811, 2655, 3646
CaiI	CAGNNNCTG	1 cut(s)	2261
CciI	TCATGA	1 cut(s)	832
CfoI	GCGC	5 cut(s)	115, 390, 919, 2365, 3520
Cfr13I	GGNCC	3 cut(s)	18, 328, 2020
Cfr9I	CCCGGG	2 cut(s)	68, 172
CseI	GACGC	2 cut(s)	1766, 2467
DinI	GGCGCC	1 cut(s)	114
DraIII	CACNNNGTG	3 cut(s)	1555, 2383, 3192
DrdI	GACNNNNNNGTC	1 cut(s)	2921
DseDI	GACNNNNNNGTC	1 cut(s)	2921
Eam1104I	CTCTTC	3 cut(s)	91, 1566, 3263
EarI	CTCTTC	3 cut(s)	91, 1566, 3263
Eco105I	TACGTA	1 cut(s)	1931
Eco130I	CCWWGG	3 cut(s)	840, 1227, 3373
Eco24I	GRGCYC	1 cut(s)	3441
Eco31I	GGTCTC	2 cut(s)	1007, 2286
Eco47I	GGWCC	3 cut(s)	18, 328, 2020
Eco47III	AGCGCT	1 cut(s)	389
Eco57I	CTGAAG	1 cut(s)	1513
Eco72I	CACGTG	1 cut(s)	2312
Eco81I	CCTNAGG	1 cut(s)	1563
Eco88I	CYCGRG	5 cut(s)	22, 68, 172, 2354, 3528
Eco91I	GGTNACC	1 cut(s)	119
EcoNI	CCTNNNNNAGG	1 cut(s)	1231
EcoO65I	GGTNACC	1 cut(s)	119
EcoRI	GAATTC	3 cut(s)	178, 662, 1523
EcoRII	CCWGG	3 cut(s)	461, 3500, 3568
EcoT14I	CCWWGG	3 cut(s)	840, 1227, 3373
EcoT22I	ATGCAT	1 cut(s)	2212
EcoT38I	GRGCYC	1 cut(s)	3441
EgeI	GGCGCC	1 cut(s)	114
EheI	GGCGCC	1 cut(s)	114
ErhI	CCWWGG	3 cut(s)	840, 1227, 3373
FalI	AAGNNNNNCTT	6 cut(s)	188, 220, 1511, 1543, 2088, 2120
FaqI	GGGAC	6 cut(s)	314, 1561, 2033, 2102, 2189, 3323
FauI	CCCGC	1 cut(s)	240
FauNDI	CATATG	1 cut(s)	1972
FbaI	TGATCA	2 cut(s)	1152, 2220
FblI	GTMKAC	3 cut(s)	80, 1169, 2449
Fnu4HI	GCNGC	9 cut(s)	65, 773, 1021, 1437, 1602, 1635, 2613, 2931, 3225
FriOI	GRGCYC	1 cut(s)	3441
Fsp4HI	GCNGC	9 cut(s)	65, 773, 1021, 1437, 1602, 1635, 2613, 2931, 3225
FspBI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
GlaI	GCGC	5 cut(s)	114, 389, 918, 2364, 3519
GluI	GCNGC	9 cut(s)	65, 773, 1021, 1437, 1602, 1635, 2613, 2931, 3225
GsaI	CCCAGC	4 cut(s)	41, 3494, 3598, 3602
GsuI	CTGGAG	1 cut(s)	908
HaeII	RGCGCY	2 cut(s)	116, 391
HaeIII	GGCC	3 cut(s)	1946, 3128, 3500
HapII	CCGG	4 cut(s)	69, 116, 173, 3090
HgaI	GACGC	2 cut(s)	1766, 2467
HhaI	GCGC	5 cut(s)	115, 390, 919, 2365, 3520
Hin1I	GRCGYC	2 cut(s)	113, 1238
Hin6I	GCGC	5 cut(s)	113, 388, 917, 2363, 3518
HinP1I	GCGC	5 cut(s)	113, 388, 917, 2363, 3518
HincII	GTYRAC	3 cut(s)	81, 2793, 3154
HindII	GTYRAC	3 cut(s)	81, 2793, 3154
HindIII	AAGCTT	2 cut(s)	1472, 2102
HpaII	CCGG	4 cut(s)	69, 116, 173, 3090
HphI	GGTGA	6 cut(s)	113, 1580, 2542, 2591, 2800, 3478
Hpy99I	CGWCG	4 cut(s)	82, 85, 1678, 1681
HpyAV	CCTTC	6 cut(s)	879, 1441, 2105, 2639, 3319, 3437
HpyCH4IV	ACGT	7 cut(s)	77, 1238, 1666, 1930, 2311, 2789, 3285
HpyF10VI	GCNNNNNNNGC	7 cut(s)	48, 112, 144, 923, 2663, 3011, 3641
HpySE526I	ACGT	7 cut(s)	77, 1238, 1666, 1930, 2311, 2789, 3285
Hsp92I	GRCGYC	2 cut(s)	113, 1238
HspAI	GCGC	5 cut(s)	113, 388, 917, 2363, 3518
KasI	GGCGCC	1 cut(s)	112
Kpn2I	TCCGGA	1 cut(s)	3089
Ksp22I	TGATCA	2 cut(s)	1152, 2220
LmnI	GCTCC	4 cut(s)	29, 72, 953, 3504
Lsp1109I	GCAGC	7 cut(s)	759, 1448, 1588, 1621, 2599, 2942, 3236
LweI	GCATC	5 cut(s)	319, 665, 1523, 1613, 2072
MaeI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
MaeII	ACGT	7 cut(s)	77, 1238, 1666, 1930, 2311, 2789, 3285
MbiI	CCGCTC	2 cut(s)	1020, 1393
MfeI	CAATTG	2 cut(s)	1032, 1335
MflI	RGATCY	4 cut(s)	523, 1916, 2065, 3378
MhlI	GDGCHC	2 cut(s)	77, 3441
Mly113I	GGCGCC	1 cut(s)	113
MmeI	TCCRAC	5 cut(s)	460, 1469, 2016, 2608, 3190
Mph1103I	ATGCAT	1 cut(s)	2212
MroI	TCCGGA	1 cut(s)	3089
MroXI	GAANNNNTTC	3 cut(s)	389, 456, 1451
MseI	TTAA	8 cut(s)	363, 498, 1326, 1761, 1958, 2154, 2319, 3038
MslI	CAYNNNNRTG	2 cut(s)	1148, 3432
MspA1I	CMGCKG	2 cut(s)	3494, 3598
MspI	CCGG	4 cut(s)	69, 116, 173, 3090
MspR9I	CCNGG	8 cut(s)	69, 70, 117, 173, 174, 463, 3502, 3570
MunI	CAATTG	2 cut(s)	1032, 1335
Mva1269I	GAATGC	2 cut(s)	859, 2655
MvaI	CCWGG	3 cut(s)	463, 3502, 3570
MvnI	CGCG	2 cut(s)	147, 917
MwoI	GCNNNNNNNGC	7 cut(s)	48, 112, 144, 923, 2663, 3011, 3641
NarI	GGCGCC	1 cut(s)	113
NciI	CCSGG	5 cut(s)	69, 70, 117, 173, 174
NdeI	CATATG	1 cut(s)	1972
NlaIV	GGNNCC	7 cut(s)	114, 330, 1297, 2021, 2351, 2587, 3380
NmuCI	GTSAC	5 cut(s)	119, 1547, 2455, 2530, 3145
NruI	TCGCGA	1 cut(s)	147
NsiI	ATGCAT	1 cut(s)	2212
NspI	RCATGY	1 cut(s)	2214
NspV	TTCGAA	1 cut(s)	872
PaeR7I	CTCGAG	2 cut(s)	22, 2354
PagI	TCATGA	1 cut(s)	832
PcsI	WCGNNNNNNNCGW	1 cut(s)	29
PctI	GAATGC	2 cut(s)	859, 2655
PdmI	GAANNNNTTC	3 cut(s)	389, 456, 1451
PfeI	GAWTC	8 cut(s)	13, 168, 1452, 1694, 1741, 2776, 2838, 3578
PfoI	TCCNGGA	1 cut(s)	3568
PkrI	GCNGC	9 cut(s)	66, 774, 1022, 1438, 1603, 1636, 2614, 2932, 3226
Ple19I	CGATCG	1 cut(s)	145
PluTI	GGCGCC	1 cut(s)	116
PmaCI	CACGTG	1 cut(s)	2312
PmlI	CACGTG	1 cut(s)	2312
Ppu21I	YACGTR	2 cut(s)	1931, 2312
PshAI	GACNNNNGTC	2 cut(s)	2445, 2728
Psp6I	CCWGG	3 cut(s)	461, 3500, 3568
PspCI	CACGTG	1 cut(s)	2312
PspEI	GGTNACC	1 cut(s)	119
PspFI	CCCAGC	4 cut(s)	37, 3490, 3594, 3598
PspGI	CCWGG	3 cut(s)	461, 3500, 3568
PspN4I	GGNNCC	7 cut(s)	114, 330, 1297, 2021, 2351, 2587, 3380
PspPI	GGNCC	3 cut(s)	18, 328, 2020
PspXI	VCTCGAGB	1 cut(s)	2354
PstI	CTGCAG	1 cut(s)	523
PstNI	CAGNNNCTG	1 cut(s)	2261
PsuI	RGATCY	4 cut(s)	523, 1916, 2065, 3378
PvuI	CGATCG	1 cut(s)	145
PvuII	CAGCTG	2 cut(s)	3494, 3598
RruI	TCGCGA	1 cut(s)	147
RseI	CAYNNNNRTG	2 cut(s)	1148, 3432
SalI	GTCGAC	1 cut(s)	79
SaqAI	TTAA	8 cut(s)	363, 498, 1326, 1761, 1958, 2154, 2319, 3038
SatI	GCNGC	9 cut(s)	65, 773, 1021, 1437, 1602, 1635, 2613, 2931, 3225
Sau96I	GGNCC	3 cut(s)	18, 328, 2020
ScaI	AGTACT	1 cut(s)	989
ScrFI	CCNGG	8 cut(s)	69, 70, 117, 173, 174, 463, 3502, 3570
SduI	GDGCHC	2 cut(s)	77, 3441
SfaNI	GCATC	5 cut(s)	319, 665, 1523, 1613, 2072
SfcI	CTRYAG	2 cut(s)	519, 1948
SfoI	GGCGCC	1 cut(s)	114
Sfr274I	CTCGAG	2 cut(s)	22, 2354
SfuI	TTCGAA	1 cut(s)	872
SgrDI	CGTCGACG	1 cut(s)	79
SinI	GGWCC	3 cut(s)	18, 328, 2020
SlaI	CTCGAG	2 cut(s)	22, 2354
SmaI	CCCGGG	2 cut(s)	70, 174
SmiMI	CAYNNNNRTG	2 cut(s)	1148, 3432
SmlI	CTYRAG	4 cut(s)	22, 2354, 2470, 3064
SmoI	CTYRAG	4 cut(s)	22, 2354, 2470, 3064
SnaBI	TACGTA	1 cut(s)	1931
SsiI	CCGC	8 cut(s)	42, 65, 124, 247, 982, 1020, 1393, 2692
SspDI	GGCGCC	1 cut(s)	112
SspMI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
StyD4I	CCNGG	8 cut(s)	67, 68, 115, 171, 172, 461, 3500, 3568
StyI	CCWWGG	3 cut(s)	840, 1227, 3373
TaiI	ACGT	7 cut(s)	80, 1241, 1669, 1933, 2314, 2792, 3288
TaqII	GACCGA	1 cut(s)	6
TatI	WGTACW	8 cut(s)	198, 705, 801, 881, 987, 1107, 1766, 1821
TauI	GCSGC	2 cut(s)	67, 1023
TfiI	GAWTC	8 cut(s)	13, 168, 1452, 1694, 1741, 2776, 2838, 3578
Tru1I	TTAA	8 cut(s)	363, 498, 1326, 1761, 1958, 2154, 2319, 3038
Tru9I	TTAA	8 cut(s)	363, 498, 1326, 1761, 1958, 2154, 2319, 3038
TseFI	GTSAC	5 cut(s)	119, 1547, 2455, 2530, 3145
TseI	GCWGC	7 cut(s)	772, 1436, 1601, 1634, 2612, 2930, 3224
Tsp45I	GTSAC	5 cut(s)	119, 1547, 2455, 2530, 3145
TspGWI	ACGGA	2 cut(s)	522, 3013
TspMI	CCCGGG	2 cut(s)	68, 172
VpaK11BI	GGWCC	3 cut(s)	18, 328, 2020
XagI	CCTNNNNNAGG	1 cut(s)	1231
XceI	RCATGY	1 cut(s)	2214
XhoI	CTCGAG	2 cut(s)	22, 2354
XmaI	CCCGGG	2 cut(s)	68, 172
XmiI	GTMKAC	3 cut(s)	80, 1169, 2449
XmnI	GAANNNNTTC	3 cut(s)	389, 456, 1451
XspI	CTAG	5 cut(s)	1233, 1893, 2399, 2667, 3335
ZraI	GACGTC	1 cut(s)	1239
ZrmI	AGTACT	1 cut(s)	989
Zsp2I	ATGCAT	1 cut(s)	2212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.