AT1G60080

3' exoribonuclease family, domain 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010687
Physical Location & Seq
Reverse (-)
22152230 .. 22154643
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60080.1

Sequence Viewer

Length: 909 bp
ATGGGAATACCAGATGCAGCTCAAGACTTGTCGACAGAGATGGAAGTTGATGCTTTTAGAAGAATCTTCCCTCTTCGTTTCTTTGAGCGTCATCTCTCTGAATCTCTTCGTCCTGATGGAAGACAACTTGGAAAAGCTAGAGACACCATTGTTAATCTTGGACTTGTTTCAACTGCTGATGGGTCTGCTCTTGCTAAGATTGGTTCCACTACTATGCTTGCTGCTATTAGAATGGAAGTTATGACTCCTTCTACGGACTCTCCAGACGAAGGATGCATAGCTATTGAGTTCCATATGCCTCCAATTTGTTCTCCGACTGTTCGTCCTGGAAGACCAGCCGAAGCTGCTCCCGTTATTTCAAAGCGTTTGTCTGATACTATTTTAAGTTCTGGTATGATTGATCTTAAAGAACTGTGTCTAGTTAGTGGAAAAGCTGCTTGGATGGGTTATCTGGACATATATTGCCTGGATGCTGATGGTGCTCTCTTTGATGCTGCTCTGCTTGCTGCTGTCGCAGCCTTCTCCAATCTGCAAATTCCAATTGTTGCTCTGAATGACAATGGAAGAATAGTTGCCGTCACTGGAGAAAAAGATCAAGACAATGCTTTGATCACCGAGAAAGAAGCAGTGAACAAGGAGAAGAGAAAACTTACGCTTAAGAACATTCCTTTCTCTTTAACTTGCATACTTCACAAGAACTACATCTTAGCAGATCCAACAACCGAAGAAGAATCGATCATGGACACTCTTGTGACTGTTGTTTTGGATTCCTCGGATCAAATGGTTTCTTTCTACAAATCAGGAGGAGCTGCTCTCGCCTATTCATCAGCCATCAAGAGCTGTGTCGAGTTAGCAAGGAAGAGAGCAAAGGAGCTTAAGCAGATATTGGGAGAGATGGATATCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
RNase_PH RNase_PH_C

Protein Analysis

302

Amino Acids

32.7

Weight (kDa)

4.96

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 46 - 180 4e-26 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 222 - 287 6.1e-11 3' exoribonuclease family, domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 32
AclWI GGATC 2 cut(s) 707, 783
AcsI RAATTY 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 269
AflII CTTAAG 2 cut(s) 656, 875
AgsI TTSAA 2 cut(s) 171, 360
AhdI GACNNNNNGTC 1 cut(s) 321
AjnI CCWGG 2 cut(s) 325, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 421, 453
AleI CACNNNNGTG 1 cut(s) 749
AluBI AGCT 8 cut(s) 20, 137, 281, 344, 434, 809, 840, 874
AluI AGCT 8 cut(s) 20, 137, 281, 344, 434, 809, 840, 874
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 2 cut(s) 707, 783
ApeKI GCWGC 8 cut(s) 17, 221, 344, 434, 494, 506, 515, 809
ApoI RAATTY 1 cut(s) 534
ArsI GACNNNNNNTTYG 4 cut(s) 353, 385, 745, 777
Asp700I GAANNNNTTC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 604
BbsI GAAGAC 2 cut(s) 127, 337
Bbv12I GWGCWC 1 cut(s) 484
BbvI GCAGC 8 cut(s) 29, 208, 331, 421, 481, 493, 527, 796
BccI CCATC 7 cut(s) 34, 110, 173, 436, 470, 839, 889
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 327, 467
BclI TGATCA 1 cut(s) 609
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 2 cut(s) 138, 419
BfrI CTTAAG 2 cut(s) 656, 875
BisI GCNGC 8 cut(s) 18, 222, 345, 435, 495, 507, 516, 810
BlsI GCNGC 8 cut(s) 19, 223, 346, 436, 496, 508, 517, 811
Bme1390I CCNGG 2 cut(s) 327, 467
BmeRI GACNNNNNGTC 1 cut(s) 321
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 327, 467
BmsI GCATC 5 cut(s) 4, 40, 263, 460, 481
BpiI GAAGAC 2 cut(s) 127, 337
BplI GAGNNNNNCTC 2 cut(s) 798, 830
BpmI CTGGAG 2 cut(s) 246, 603
BpuEI CTTGAG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 734
BsaBI GATNNNNATC 1 cut(s) 899
BsaJI CCNNGG 1 cut(s) 771
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 586
Bse8I GATNNNNATC 1 cut(s) 899
BseBI CCWGG 2 cut(s) 327, 467
BseCI ATCGAT 1 cut(s) 734
BseDI CCNNGG 1 cut(s) 771
BseGI GGATG 3 cut(s) 278, 447, 475
BseJI GATNNNNATC 1 cut(s) 899
BseLI CCNNNNNNNGG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 8 cut(s) 29, 208, 331, 421, 481, 493, 527, 796
BshVI ATCGAT 1 cut(s) 734
BsiHKAI GWGCWC 1 cut(s) 484
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 6 cut(s) 400, 592, 609, 712, 735, 775
BspDI ATCGAT 1 cut(s) 734
BspLI GGNNCC 1 cut(s) 205
BspPI GGATC 2 cut(s) 707, 783
BspTI CTTAAG 2 cut(s) 656, 875
BsrI ACTGG 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 771
BssMI GATC 6 cut(s) 400, 592, 609, 712, 735, 775
Bst2UI CCWGG 2 cut(s) 327, 467
Bst4CI ACNGT 3 cut(s) 319, 414, 757
Bst6I CTCTTC 4 cut(s) 78, 111, 635, 854
BstAFI CTTAAG 2 cut(s) 656, 875
BstC8I GCNNGC 2 cut(s) 219, 504
BstDEI CTNAG 2 cut(s) 195, 706
BstF5I GGATG 3 cut(s) 278, 447, 475
BstKTI GATC 6 cut(s) 403, 595, 612, 715, 738, 778
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 6 cut(s) 400, 592, 609, 712, 735, 775
BstMWI GCNNNNNNNGC 6 cut(s) 344, 479, 503, 512, 515, 815
BstNI CCWGG 2 cut(s) 327, 467
BstSCI CCNGG 2 cut(s) 325, 465
BstV1I GCAGC 8 cut(s) 29, 208, 331, 421, 481, 493, 527, 796
BstV2I GAAGAC 2 cut(s) 127, 337
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
Bsu15I ATCGAT 1 cut(s) 734
BsuTUI ATCGAT 1 cut(s) 734
BtsCI GGATG 3 cut(s) 278, 447, 475
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 579, 633
Cac8I GCNNGC 2 cut(s) 219, 504
ClaI ATCGAT 1 cut(s) 734
CseI GACGC 1 cut(s) 77
CviAII CATG 1 cut(s) 739
DdeI CTNAG 2 cut(s) 195, 706
DpnI GATC 6 cut(s) 402, 594, 611, 714, 737, 777
DpnII GATC 6 cut(s) 400, 592, 609, 712, 735, 775
DriI GACNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 4 cut(s) 78, 111, 635, 854
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 4 cut(s) 78, 111, 635, 854
Eco32I GATATC 1 cut(s) 901
EcoRII CCWGG 2 cut(s) 325, 465
EcoRV GATATC 1 cut(s) 901
EcoT22I ATGCAT 1 cut(s) 278
FaeI CATG 1 cut(s) 742
FatI CATG 1 cut(s) 738
FauNDI CATATG 1 cut(s) 294
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 8 cut(s) 18, 222, 345, 435, 495, 507, 516, 810
FokI GGATG 3 cut(s) 285, 454, 482
Fsp4HI GCNGC 8 cut(s) 18, 222, 345, 435, 495, 507, 516, 810
FspBI CTAG 2 cut(s) 138, 419
GluI GCNGC 8 cut(s) 18, 222, 345, 435, 495, 507, 516, 810
GsuI CTGGAG 2 cut(s) 246, 603
HgaI GACGC 1 cut(s) 77
Hin1II CATG 1 cut(s) 742
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 6 cut(s) 63, 101, 244, 257, 731, 767
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 2 cut(s) 33, 631
Hpy188I TCNGA 5 cut(s) 100, 315, 373, 552, 775
Hpy188III TCNNGA 7 cut(s) 23, 113, 263, 452, 596, 801, 835
Hpy8I GTNNAC 2 cut(s) 33, 631
HpyAV CCTTC 3 cut(s) 258, 263, 529
HpyCH4III ACNGT 3 cut(s) 319, 414, 757
HpyCH4V TGCA 4 cut(s) 17, 276, 532, 684
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 479, 503, 512, 515, 815
HpyF3I CTNAG 2 cut(s) 195, 706
Hsp92II CATG 1 cut(s) 742
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 6 cut(s) 400, 592, 609, 712, 735, 775
LmnI GCTCC 3 cut(s) 352, 806, 871
Lsp1109I GCAGC 8 cut(s) 29, 208, 331, 421, 481, 493, 527, 796
LweI GCATC 5 cut(s) 4, 40, 263, 460, 481
MaeI CTAG 2 cut(s) 138, 419
MaeIII GTNAC 2 cut(s) 577, 751
MalI GATC 6 cut(s) 402, 594, 611, 714, 737, 777
MboI GATC 6 cut(s) 400, 592, 609, 712, 735, 775
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 3 cut(s) 303, 534, 540
MlyI GAGTC 2 cut(s) 238, 251
MmeI TCCRAC 2 cut(s) 338, 740
MnlI CCTC 4 cut(s) 81, 309, 781, 797
Mph1103I ATGCAT 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 6 cut(s) 153, 383, 405, 657, 677, 876
MslI CAYNNNNRTG 2 cut(s) 212, 749
MspCI CTTAAG 2 cut(s) 656, 875
MspR9I CCNGG 2 cut(s) 327, 467
MunI CAATTG 1 cut(s) 540
MvaI CCWGG 2 cut(s) 327, 467
MwoI GCNNNNNNNGC 6 cut(s) 344, 479, 503, 512, 515, 815
NdeI CATATG 1 cut(s) 294
NdeII GATC 6 cut(s) 400, 592, 609, 712, 735, 775
NlaIII CATG 1 cut(s) 742
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 577, 751
NsiI ATGCAT 1 cut(s) 278
OliI CACNNNNGTG 1 cut(s) 749
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 4 cut(s) 63, 101, 731, 767
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 8 cut(s) 19, 223, 346, 436, 496, 508, 517, 811
PleI GAGTC 2 cut(s) 238, 251
PpsI GAGTC 2 cut(s) 238, 251
Psp6I CCWGG 2 cut(s) 325, 465
PspGI CCWGG 2 cut(s) 325, 465
PspN4I GGNNCC 1 cut(s) 205
PsuI RGATCY 1 cut(s) 712
RseI CAYNNNNRTG 2 cut(s) 212, 749
SalI GTCGAC 1 cut(s) 31
SaqAI TTAA 6 cut(s) 153, 383, 405, 657, 677, 876
SatI GCNGC 8 cut(s) 18, 222, 345, 435, 495, 507, 516, 810
Sau3AI GATC 6 cut(s) 400, 592, 609, 712, 735, 775
SchI GAGTC 2 cut(s) 238, 251
ScrFI CCNGG 2 cut(s) 327, 467
SduI GDGCHC 1 cut(s) 484
SetI ASST 8 cut(s) 22, 139, 283, 346, 436, 811, 842, 876
SfaNI GCATC 5 cut(s) 4, 40, 263, 460, 481
SmiMI CAYNNNNRTG 2 cut(s) 212, 749
SmlI CTYRAG 3 cut(s) 21, 656, 875
SmoI CTYRAG 3 cut(s) 21, 656, 875
Sse9I AATT 3 cut(s) 303, 534, 540
SspMI CTAG 2 cut(s) 138, 419
StyD4I CCNGG 2 cut(s) 325, 465
TaaI ACNGT 3 cut(s) 319, 414, 757
TaqI TCGA 4 cut(s) 32, 734, 846, 903
TasI AATT 3 cut(s) 303, 534, 540
TfiI GAWTC 4 cut(s) 63, 101, 731, 767
Tru1I TTAA 6 cut(s) 153, 383, 405, 657, 677, 876
Tru9I TTAA 6 cut(s) 153, 383, 405, 657, 677, 876
TscAI CASTG 2 cut(s) 586, 633
TseFI GTSAC 2 cut(s) 577, 751
TseI GCWGC 8 cut(s) 17, 221, 344, 434, 494, 506, 515, 809
Tsp45I GTSAC 2 cut(s) 577, 751
TspDTI ATGAA 1 cut(s) 813
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 2 cut(s) 586, 633
Vha464I CTTAAG 2 cut(s) 656, 875
XapI RAATTY 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 2 cut(s) 138, 419
Zsp2I ATGCAT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.