Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 833 |
| AciI | CCGC | 3 cut(s) | 391, 519, 833 |
| AclWI | GGATC | 3 cut(s) | 365, 378, 458 |
| AcuI | CTGAAG | 3 cut(s) | 440, 564, 932 |
| AfaI | GTAC | 2 cut(s) | 694, 1159 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 473, 1200, 1201 |
| AgsI | TTSAA | 3 cut(s) | 431, 482, 1067 |
| AhlI | ACTAGT | 1 cut(s) | 826 |
| AjiI | CACGTC | 1 cut(s) | 305 |
| AluBI | AGCT | 5 cut(s) | 124, 418, 542, 550, 1096 |
| AluI | AGCT | 5 cut(s) | 124, 418, 542, 550, 1096 |
| Alw21I | GWGCWC | 3 cut(s) | 165, 826, 1108 |
| Alw26I | GTCTC | 3 cut(s) | 66, 76, 1104 |
| AlwI | GGATC | 3 cut(s) | 365, 378, 458 |
| Ama87I | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| AoxI | GGCC | 3 cut(s) | 177, 1116, 1191 |
| ApeKI | GCWGC | 3 cut(s) | 31, 539, 1096 |
| AspS9I | GGNCC | 3 cut(s) | 183, 1117, 1192 |
| AsuC2I | CCSGG | 2 cut(s) | 181, 348 |
| AsuHPI | GGTGA | 2 cut(s) | 362, 473 |
| AvaI | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| AvaII | GGWCC | 1 cut(s) | 183 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 882, 915 |
| BamHI | GGATCC | 1 cut(s) | 370 |
| BanII | GRGCYC | 1 cut(s) | 1063 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 685, 717 |
| Bbv12I | GWGCWC | 3 cut(s) | 165, 826, 1108 |
| BbvI | GCAGC | 3 cut(s) | 18, 551, 1083 |
| BccI | CCATC | 6 cut(s) | 51, 452, 486, 735, 887, 984 |
| BciVI | GTATCC | 1 cut(s) | 883 |
| BcnI | CCSGG | 2 cut(s) | 181, 348 |
| BcoDI | GTCTC | 3 cut(s) | 66, 76, 1104 |
| BcuI | ACTAGT | 1 cut(s) | 826 |
| BfaI | CTAG | 3 cut(s) | 807, 827, 930 |
| BfmI | CTRYAG | 2 cut(s) | 686, 894 |
| BfuI | GTATCC | 1 cut(s) | 883 |
| BisI | GCNGC | 4 cut(s) | 32, 392, 540, 1097 |
| BlsI | GCNGC | 4 cut(s) | 33, 393, 541, 1098 |
| BmcAI | AGTACT | 1 cut(s) | 1159 |
| Bme1390I | CCNGG | 2 cut(s) | 181, 348 |
| Bme18I | GGWCC | 1 cut(s) | 183 |
| BmeT110I | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| BmgBI | CACGTC | 1 cut(s) | 305 |
| BmgT120I | GGNCC | 3 cut(s) | 183, 1117, 1192 |
| BmiI | GGNNCC | 4 cut(s) | 184, 372, 877, 964 |
| BmrFI | CCNGG | 2 cut(s) | 181, 348 |
| BmsI | GCATC | 1 cut(s) | 707 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 46, 78 |
| BpuMI | CCSGG | 2 cut(s) | 181, 348 |
| BsaBI | GATNNNNATC | 3 cut(s) | 291, 462, 468 |
| BsaI | GGTCTC | 2 cut(s) | 66, 76 |
| BsaJI | CCNNGG | 1 cut(s) | 405 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 473, 1200, 1201 |
| Bse1I | ACTGG | 1 cut(s) | 905 |
| Bse8I | GATNNNNATC | 3 cut(s) | 291, 462, 468 |
| BseDI | CCNNGG | 1 cut(s) | 405 |
| BseGI | GGATG | 2 cut(s) | 82, 292 |
| BseJI | GATNNNNATC | 3 cut(s) | 291, 462, 468 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 473, 1200, 1201 |
| BseMII | CTCAG | 4 cut(s) | 22, 32, 807, 1114 |
| BseNI | ACTGG | 1 cut(s) | 905 |
| BseXI | GCAGC | 3 cut(s) | 18, 551, 1083 |
| Bsh1236I | CGCG | 1 cut(s) | 833 |
| BshFI | GGCC | 3 cut(s) | 179, 1118, 1193 |
| BsiHKAI | GWGCWC | 3 cut(s) | 165, 826, 1108 |
| BsiHKCI | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| BsiSI | CCGG | 2 cut(s) | 180, 348 |
| BslFI | GGGAC | 3 cut(s) | 196, 330, 849 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 473, 1200, 1201 |
| BsmAI | GTCTC | 3 cut(s) | 66, 76, 1104 |
| BsmFI | GGGAC | 3 cut(s) | 196, 330, 849 |
| BsnI | GGCC | 3 cut(s) | 179, 1118, 1193 |
| Bso31I | GGTCTC | 2 cut(s) | 66, 76 |
| BsoBI | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| Bsp1286I | GDGCHC | 4 cut(s) | 165, 826, 1063, 1108 |
| Bsp143I | GATC | 4 cut(s) | 311, 370, 463, 501 |
| BspACI | CCGC | 3 cut(s) | 391, 519, 833 |
| BspANI | GGCC | 3 cut(s) | 179, 1118, 1193 |
| BspCNI | CTCAG | 4 cut(s) | 21, 33, 808, 1113 |
| BspFNI | CGCG | 1 cut(s) | 833 |
| BspLI | GGNNCC | 4 cut(s) | 184, 372, 877, 964 |
| BspPI | GGATC | 3 cut(s) | 365, 378, 458 |
| BspQI | GCTCTTC | 1 cut(s) | 540 |
| BspTNI | GGTCTC | 2 cut(s) | 66, 76 |
| BsrI | ACTGG | 1 cut(s) | 905 |
| BssECI | CCNNGG | 1 cut(s) | 405 |
| BssMI | GATC | 4 cut(s) | 311, 370, 463, 501 |
| Bst4CI | ACNGT | 3 cut(s) | 627, 898, 1129 |
| Bst6I | CTCTTC | 3 cut(s) | 480, 540, 648 |
| BstC8I | GCNNGC | 2 cut(s) | 23, 552 |
| BstDEI | CTNAG | 5 cut(s) | 8, 41, 816, 1085, 1100 |
| BstEII | GGTNACC | 1 cut(s) | 350 |
| BstF5I | GGATG | 2 cut(s) | 82, 292 |
| BstFNI | CGCG | 1 cut(s) | 833 |
| BstKTI | GATC | 4 cut(s) | 314, 373, 466, 504 |
| BstMAI | GTCTC | 3 cut(s) | 66, 76, 1104 |
| BstMBI | GATC | 4 cut(s) | 311, 370, 463, 501 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 13, 22, 31, 224, 397, 830 |
| BstPI | GGTNACC | 1 cut(s) | 350 |
| BstSCI | CCNGG | 2 cut(s) | 179, 346 |
| BstSFI | CTRYAG | 2 cut(s) | 686, 894 |
| BstUI | CGCG | 1 cut(s) | 833 |
| BstV1I | GCAGC | 3 cut(s) | 18, 551, 1083 |
| BstX2I | RGATCY | 1 cut(s) | 370 |
| BstYI | RGATCY | 1 cut(s) | 370 |
| BsuI | GTATCC | 1 cut(s) | 883 |
| BsuRI | GGCC | 3 cut(s) | 179, 1118, 1193 |
| BtgZI | GCGATG | 2 cut(s) | 99, 471 |
| BtrI | CACGTC | 1 cut(s) | 305 |
| BtsCI | GGATG | 2 cut(s) | 82, 292 |
| BtsIMutI | CAGTG | 2 cut(s) | 38, 1108 |
| Cac8I | GCNNGC | 2 cut(s) | 23, 552 |
| Cfr13I | GGNCC | 3 cut(s) | 183, 1117, 1192 |
| Csp6I | GTAC | 2 cut(s) | 693, 1158 |
| CviAII | CATG | 2 cut(s) | 340, 1114 |
| CviQI | GTAC | 2 cut(s) | 693, 1158 |
| DdeI | CTNAG | 5 cut(s) | 8, 41, 816, 1085, 1100 |
| DpnI | GATC | 4 cut(s) | 313, 372, 465, 503 |
| DpnII | GATC | 4 cut(s) | 311, 370, 463, 501 |
| Eam1104I | CTCTTC | 3 cut(s) | 480, 540, 648 |
| EarI | CTCTTC | 3 cut(s) | 480, 540, 648 |
| Eco24I | GRGCYC | 1 cut(s) | 1063 |
| Eco31I | GGTCTC | 2 cut(s) | 66, 76 |
| Eco32I | GATATC | 1 cut(s) | 1078 |
| Eco47I | GGWCC | 1 cut(s) | 183 |
| Eco57I | CTGAAG | 3 cut(s) | 440, 564, 932 |
| Eco88I | CYCGRG | 3 cut(s) | 164, 1056, 1107 |
| Eco91I | GGTNACC | 1 cut(s) | 350 |
| EcoO65I | GGTNACC | 1 cut(s) | 350 |
| EcoRV | GATATC | 1 cut(s) | 1078 |
| EcoT38I | GRGCYC | 1 cut(s) | 1063 |
| FaeI | CATG | 2 cut(s) | 343, 1117 |
| FaqI | GGGAC | 3 cut(s) | 196, 330, 849 |
| FatI | CATG | 2 cut(s) | 339, 1113 |
| FauI | CCCGC | 1 cut(s) | 826 |
| FauNDI | CATATG | 1 cut(s) | 316 |
| Fnu4HI | GCNGC | 4 cut(s) | 32, 392, 540, 1097 |
| FokI | GGATG | 2 cut(s) | 89, 299 |
| FriOI | GRGCYC | 1 cut(s) | 1063 |
| Fsp4HI | GCNGC | 4 cut(s) | 32, 392, 540, 1097 |
| FspBI | CTAG | 3 cut(s) | 807, 827, 930 |
| GluI | GCNGC | 4 cut(s) | 32, 392, 540, 1097 |
| HaeIII | GGCC | 3 cut(s) | 179, 1118, 1193 |
| HapII | CCGG | 2 cut(s) | 180, 348 |
| Hin1II | CATG | 2 cut(s) | 343, 1117 |
| HindIII | AAGCTT | 1 cut(s) | 122 |
| HinfI | GANTC | 6 cut(s) | 320, 343, 431, 572, 976, 1067 |
| HpaII | CCGG | 2 cut(s) | 180, 348 |
| HphI | GGTGA | 2 cut(s) | 362, 473 |
| Hpy166II | GTNNAC | 3 cut(s) | 299, 330, 845 |
| Hpy188I | TCNGA | 7 cut(s) | 11, 76, 387, 817, 951, 989, 1234 |
| Hpy188III | TCNNGA | 4 cut(s) | 166, 563, 1109, 1217 |
| Hpy8I | GTNNAC | 3 cut(s) | 299, 330, 845 |
| Hpy99I | CGWCG | 2 cut(s) | 274, 306 |
| HpyAV | CCTTC | 4 cut(s) | 74, 163, 711, 976 |
| HpyCH4III | ACNGT | 3 cut(s) | 627, 898, 1129 |
| HpyCH4IV | ACGT | 1 cut(s) | 304 |
| HpyCH4V | TGCA | 5 cut(s) | 21, 251, 400, 539, 902 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 13, 22, 31, 224, 397, 830 |
| HpyF3I | CTNAG | 5 cut(s) | 8, 41, 816, 1085, 1100 |
| HpySE526I | ACGT | 1 cut(s) | 304 |
| Hsp92II | CATG | 2 cut(s) | 343, 1117 |
| Kzo9I | GATC | 4 cut(s) | 311, 370, 463, 501 |
| LguI | GCTCTTC | 1 cut(s) | 540 |
| LmnI | GCTCC | 2 cut(s) | 559, 875 |
| Lsp1109I | GCAGC | 3 cut(s) | 18, 551, 1083 |
| LweI | GCATC | 1 cut(s) | 707 |
| MaeI | CTAG | 3 cut(s) | 807, 827, 930 |
| MaeII | ACGT | 1 cut(s) | 304 |
| MaeIII | GTNAC | 2 cut(s) | 350, 800 |
| MalI | GATC | 4 cut(s) | 313, 372, 465, 503 |
| MboI | GATC | 4 cut(s) | 311, 370, 463, 501 |
| MboII | GAAGA | 9 cut(s) | 321, 494, 497, 557, 665, 693, 750, 796, 1221 |
| MflI | RGATCY | 1 cut(s) | 370 |
| MhlI | GDGCHC | 4 cut(s) | 165, 826, 1063, 1108 |
| MluCI | AATT | 6 cut(s) | 150, 477, 674, 794, 850, 1146 |
| MlyI | GAGTC | 1 cut(s) | 352 |
| MseI | TTAA | 3 cut(s) | 849, 884, 918 |
| MspA1I | CMGCKG | 1 cut(s) | 542 |
| MspI | CCGG | 2 cut(s) | 180, 348 |
| MspR9I | CCNGG | 2 cut(s) | 181, 348 |
| MvnI | CGCG | 1 cut(s) | 833 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 13, 22, 31, 224, 397, 830 |
| NciI | CCSGG | 2 cut(s) | 181, 348 |
| NdeI | CATATG | 1 cut(s) | 316 |
| NdeII | GATC | 4 cut(s) | 311, 370, 463, 501 |
| NlaIII | CATG | 2 cut(s) | 343, 1117 |
| NlaIV | GGNNCC | 4 cut(s) | 184, 372, 877, 964 |
| NmuCI | GTSAC | 1 cut(s) | 350 |
| PaeR7I | CTCGAG | 3 cut(s) | 164, 1056, 1107 |
| PciSI | GCTCTTC | 1 cut(s) | 540 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 301, 391, 453 |
| PfeI | GAWTC | 5 cut(s) | 320, 431, 572, 976, 1067 |
| PkrI | GCNGC | 4 cut(s) | 33, 393, 541, 1098 |
| PleI | GAGTC | 1 cut(s) | 351 |
| PpsI | GAGTC | 1 cut(s) | 351 |
| PspEI | GGTNACC | 1 cut(s) | 350 |
| PspN4I | GGNNCC | 4 cut(s) | 184, 372, 877, 964 |
| PspPI | GGNCC | 3 cut(s) | 183, 1117, 1192 |
| PspXI | VCTCGAGB | 1 cut(s) | 1056 |
| PsuI | RGATCY | 1 cut(s) | 370 |
| PvuII | CAGCTG | 1 cut(s) | 542 |
| RsaI | GTAC | 2 cut(s) | 694, 1159 |
| RsaNI | GTAC | 2 cut(s) | 693, 1158 |
| SapI | GCTCTTC | 1 cut(s) | 540 |
| SaqAI | TTAA | 3 cut(s) | 849, 884, 918 |
| SatI | GCNGC | 4 cut(s) | 32, 392, 540, 1097 |
| Sau3AI | GATC | 4 cut(s) | 311, 370, 463, 501 |
| Sau96I | GGNCC | 3 cut(s) | 183, 1117, 1192 |
| ScaI | AGTACT | 1 cut(s) | 1159 |
| SchI | GAGTC | 1 cut(s) | 352 |
| ScrFI | CCNGG | 2 cut(s) | 181, 348 |
| SduI | GDGCHC | 4 cut(s) | 165, 826, 1063, 1108 |
| SetI | ASST | 9 cut(s) | 72, 126, 307, 357, 420, 544, 552, 616, 1098 |
| SfaNI | GCATC | 1 cut(s) | 707 |
| SfcI | CTRYAG | 2 cut(s) | 686, 894 |
| Sfr274I | CTCGAG | 3 cut(s) | 164, 1056, 1107 |
| SinI | GGWCC | 1 cut(s) | 183 |
| SlaI | CTCGAG | 3 cut(s) | 164, 1056, 1107 |
| SmlI | CTYRAG | 3 cut(s) | 164, 1056, 1107 |
| SmoI | CTYRAG | 3 cut(s) | 164, 1056, 1107 |
| SpeI | ACTAGT | 1 cut(s) | 826 |
| Sse9I | AATT | 6 cut(s) | 150, 477, 674, 794, 850, 1146 |
| SsiI | CCGC | 3 cut(s) | 391, 519, 833 |
| SspI | AATATT | 1 cut(s) | 773 |
| SspMI | CTAG | 3 cut(s) | 807, 827, 930 |
| StyD4I | CCNGG | 2 cut(s) | 179, 346 |
| TaaI | ACNGT | 3 cut(s) | 627, 898, 1129 |
| TaiI | ACGT | 1 cut(s) | 307 |
| TaqI | TCGA | 7 cut(s) | 115, 142, 165, 272, 1057, 1108, 1216 |
| TaqII | GACCGA | 1 cut(s) | 200 |
| TasI | AATT | 6 cut(s) | 150, 477, 674, 794, 850, 1146 |
| TatI | WGTACW | 1 cut(s) | 1157 |
| TauI | GCSGC | 1 cut(s) | 394 |
| TfiI | GAWTC | 5 cut(s) | 320, 431, 572, 976, 1067 |
| Tru1I | TTAA | 3 cut(s) | 849, 884, 918 |
| Tru9I | TTAA | 3 cut(s) | 849, 884, 918 |
| TscAI | CASTG | 2 cut(s) | 45, 1108 |
| TseFI | GTSAC | 1 cut(s) | 350 |
| TseI | GCWGC | 3 cut(s) | 31, 539, 1096 |
| Tsp45I | GTSAC | 1 cut(s) | 350 |
| TspDTI | ATGAA | 7 cut(s) | 93, 195, 605, 612, 666, 913, 985 |
| TspRI | CASTG | 2 cut(s) | 45, 1108 |
| VpaK11BI | GGWCC | 1 cut(s) | 183 |
| XhoI | CTCGAG | 3 cut(s) | 164, 1056, 1107 |
| XspI | CTAG | 3 cut(s) | 807, 827, 930 |
| ZrmI | AGTACT | 1 cut(s) | 1159 |