pycom14g05860

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001156
Physical Location & Seq
Reverse (-)
5701332 .. 5703150
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05860.1

Sequence Viewer

Length: 1770 bp
ATGTGGACGAAAGTGTCAGTGGACTTTGATATCACTAGATTGGCCAAAGAGATCCTTCACTCTGCATCAGGTACAAAGATCAGTGAAGGGTTGTCTCCGCATCAGTTGCAAGAACAACTCCGAGATGCTTTAAAGGATAAGAAATTTCTGCTTGTCTTGGATGATGTGTGGAACGAAGATCGTAACAAATGGTGCCAGTTGAGAGATATGTTGACAGAGGGGGCCAAGGTAGGAACTAAGGTTTTGGTGACGACACGGAATAGCTCAGTTGCTTCAATCATGGGCACGGTTGAAAGCATAAATTTAGATGTTCTCTCTTTTGAGGATTGTTTGTCTTTGTTTGTAAAGTGTGCATTTAAAAAAGGACAAGAAAGGCAGCATCCAAGCCTCTATGAAATGGGAAAAGATATTGTAAGAAGGTGCGGAGGGGTTCCCGTAGCTGTGAAAACCTTAGGTAGTCAACTTTACTCAAAGACTGATGAGCGTCAATGGAAATTGATTAGAGATTCTGAGATATGGGAATTAGAACGAGATGGAGCTGGTCACATTCTACCTGCTTTGAGATTGAGTTATAATCAATTGCCCACTCATTTGAAGCAATGCCTTGTTTGCTGTTCAATTCTTTCAAAGAATTACATTGAATTTAATACCATGGCATTGATCGATCAAACACCTTTGCATGATGTTGGTGAGCTATATTTCAAAGAGTTATGGGACAGATCTTTCTTTCAAAATGTTAAGGATTACGCTTTCTTCTATGAATTTGATATGCATGATCTCATCCATGACCTTGTACAATCAGTCGCACAAGGTGAGAGTTTGACAGTAGACTCTGCAAGCACCAAAGGCGTCTCTGAAAATGTTAGACATCTAACATTTTTGGAAGTTGGCCAAAATGTTTCAACAACCTTGCAAAAGTTGAATAAAGTGCGGACTATGTTAGTAGAAAGTTGTGAAAACATTGATCAATCCTTCCTCAGCACTTGCATTTCAAGATTCAAATATCTGCGACTGCTTAAGTTAACCAATGCATCTTGGGAATTGTTGCCGACTTCCATTGGTTCTTTGAAGCATTTGAGAAGCCTGAACTTGGCTAGAAATGAAAGAATCACCGCACTACCCAATTCAATTTGTAAGCTGCAGAGCTTGCAAATTCTGGTTCTCCGTGGATGCGTGAATCTTGAAGAGTTCCCTAGAGACATGAGAAACTTGATCAACCTCAGATGGCTTGTAATAACCACAAAACAATCAAGTTTTCCAGAGAATGGAGTGGGATGCTTGACATCACTTCGATATCTTTCTATCAGGGAGTGTAGTAATCTAACGTGTTTGCCGCGTGATACGAGTTATCTCGCGTCGTTACGTACTCTGATGATAGGCGATTGCAAACAACTTGATTTGGTGATGGAAAACTGTCAGCTAATTCCACTGAGGCTCCAAAAATTGGGGATTATAGGAGTACCACGAATGGTGGCATTGCCTGAATGGTTTCAAGGATGCACAAACACACTACGAGTCTTGGTTATTTGGGATTGTGAGAATTTGGAGGCATTACCTGGGTGGCTGACGAGTTTCACATCGCTTCGCAGGCTTACGCTCGGTTCATGTCCCAAACTGTTGTCTTTGCCAGAGGAGATGCATCGCCTCACTACCTTAACAGAGGTTAGGATTGTAGATTGCCCTGATTTGGAGAGGAGATGTCAGCGCGACACAGGAGAAGATTGGCCAAAGATTTCTCATGTTCCAAATGTTTCTTTTGAAGGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

590

Amino Acids

67.24

Weight (kDa)

7.13

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 121 3.1e-18 NB-ARC domain
WHD_DRP PF23559 224 - 265 4e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 311 - 533 4.7e-21 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 192
AccB7I CCANNNNNTGG 2 cut(s) 1265, 1444
AccI GTMKAC 1 cut(s) 828
AccII CGCG 3 cut(s) 1336, 1355, 1707
AciI CCGC 5 cut(s) 98, 423, 931, 1113, 1334
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 3 cut(s) 42, 889, 1724
AcsI RAATTY 6 cut(s) 143, 301, 641, 761, 1152, 1540
AcyI GRCGYC 1 cut(s) 849
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 4 cut(s) 73, 795, 1366, 1461
AfiI CCNNNNNNNGG 4 cut(s) 1090, 1265, 1444, 1687
AflII CTTAAG 1 cut(s) 1016
AflIII ACRYGT 1 cut(s) 1325
AjnI CCWGG 1 cut(s) 1555
AluBI AGCT 7 cut(s) 264, 440, 539, 694, 1138, 1146, 1420
AluI AGCT 7 cut(s) 264, 440, 539, 694, 1138, 1146, 1420
Alw26I GTCTC 3 cut(s) 99, 856, 1191
AlwI GGATC 1 cut(s) 46
AoxI GGCC 4 cut(s) 42, 222, 889, 1724
ApeKI GCWGC 2 cut(s) 376, 1138
ApoI RAATTY 6 cut(s) 143, 301, 641, 761, 1152, 1540
Asp700I GAANNNNTTC 1 cut(s) 1488
AspLEI GCGC 1 cut(s) 1707
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 5 cut(s) 259, 701, 824, 1102, 1414
AxyI CCTNAGG 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 287
BalI TGGCCA 3 cut(s) 44, 891, 1726
BanI GGYRCC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 977
BbvI GCAGC 2 cut(s) 388, 1125
BccI CCATC 3 cut(s) 527, 1218, 1399
BciT130I CCWGG 1 cut(s) 1557
BclI TGATCA 2 cut(s) 964, 1212
BcoDI GTCTC 3 cut(s) 99, 856, 1191
BfaI CTAG 3 cut(s) 36, 1095, 1194
BfmI CTRYAG 1 cut(s) 1139
BfrI CTTAAG 1 cut(s) 1016
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 719
BisI GCNGC 3 cut(s) 377, 1139, 1334
BlsI GCNGC 3 cut(s) 378, 1140, 1335
Bme1390I CCNGG 1 cut(s) 1557
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 4 cut(s) 194, 223, 432, 1436
BmrFI CCNGG 1 cut(s) 1557
Bpu10I CCTNAGC 1 cut(s) 977
Bsa29I ATCGAT 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 1364
BsaHI GRCGYC 1 cut(s) 849
BsaJI CCNNGG 4 cut(s) 225, 651, 1165, 1556
BsaXI ACNNNNNCTCC 2 cut(s) 1419, 1449
Bsc4I CCNNNNNNNGG 4 cut(s) 1090, 1265, 1444, 1687
Bse1I ACTGG 1 cut(s) 196
Bse21I CCTNAGG 1 cut(s) 451
Bse3DI GCAATG 2 cut(s) 605, 1475
BseBI CCWGG 1 cut(s) 1557
BseCI ATCGAT 1 cut(s) 663
BseDI CCNNGG 4 cut(s) 225, 651, 1165, 1556
BseGI GGATG 6 cut(s) 166, 379, 780, 1175, 1280, 1502
BseLI CCNNNNNNNGG 4 cut(s) 1090, 1265, 1444, 1687
BseMI GCAATG 2 cut(s) 605, 1475
BseMII CTCAG 5 cut(s) 279, 501, 991, 1234, 1421
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 2 cut(s) 1646, 1708
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 2 cut(s) 388, 1125
Bsh1236I CGCG 3 cut(s) 1336, 1355, 1707
BshFI GGCC 4 cut(s) 44, 224, 891, 1726
BshNI GGYRCC 1 cut(s) 192
BshVI ATCGAT 1 cut(s) 663
BslFI GGGAC 2 cut(s) 728, 1593
BslI CCNNNNNNNGG 4 cut(s) 1090, 1265, 1444, 1687
BsmAI GTCTC 3 cut(s) 99, 856, 1191
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 2 cut(s) 728, 1593
BsnI GGCC 4 cut(s) 44, 224, 891, 1726
Bsp1286I GDGCHC 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 793
Bsp143I GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 5 cut(s) 98, 423, 931, 1113, 1334
BspANI GGCC 4 cut(s) 44, 224, 891, 1726
BspCNI CTCAG 5 cut(s) 278, 502, 990, 1233, 1422
BspDI ATCGAT 1 cut(s) 663
BspFNI CGCG 3 cut(s) 1336, 1355, 1707
BspLI GGNNCC 4 cut(s) 194, 223, 432, 1436
BspMAI CTGCAG 1 cut(s) 1143
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 192
BspTI CTTAAG 1 cut(s) 1016
BsrDI GCAATG 2 cut(s) 605, 1475
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 4 cut(s) 225, 651, 1165, 1556
BssMI GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
BssNI GRCGYC 1 cut(s) 849
BssT1I CCWWGG 2 cut(s) 225, 651
Bst2UI CCWGG 1 cut(s) 1557
Bst4CI ACNGT 4 cut(s) 289, 826, 1415, 1617
Bst6I CTCTTC 1 cut(s) 1179
BstACI GRCGYC 1 cut(s) 849
BstAFI CTTAAG 1 cut(s) 1016
BstAPI GCANNNNNTGC 2 cut(s) 106, 1147
BstAUI TGTACA 1 cut(s) 793
BstBAI YACGTR 1 cut(s) 1364
BstC8I GCNNGC 3 cut(s) 838, 1148, 1589
BstDEI CTNAG 7 cut(s) 237, 265, 451, 510, 977, 1220, 1430
BstDSI CCRYGG 2 cut(s) 651, 1165
BstF5I GGATG 6 cut(s) 166, 379, 780, 1175, 1280, 1502
BstFNI CGCG 3 cut(s) 1336, 1355, 1707
BstHHI GCGC 1 cut(s) 1707
BstKTI GATC 9 cut(s) 54, 81, 181, 663, 667, 722, 778, 967, 1215
BstMAI GTCTC 3 cut(s) 99, 856, 1191
BstMBI GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
BstMWI GCNNNNNNNGC 5 cut(s) 106, 609, 846, 1147, 1588
BstNI CCWGG 1 cut(s) 1557
BstSCI CCNGG 1 cut(s) 1555
BstSFI CTRYAG 1 cut(s) 1139
BstSLI GKGCMC 1 cut(s) 287
BstSNI TACGTA 1 cut(s) 1364
BstUI CGCG 3 cut(s) 1336, 1355, 1707
BstV1I GCAGC 2 cut(s) 388, 1125
BstX2I RGATCY 2 cut(s) 51, 719
BstYI RGATCY 2 cut(s) 51, 719
Bsu15I ATCGAT 1 cut(s) 663
Bsu36I CCTNAGG 1 cut(s) 451
BsuRI GGCC 4 cut(s) 44, 224, 891, 1726
BsuTUI ATCGAT 1 cut(s) 663
BtgI CCRYGG 2 cut(s) 651, 1165
BtgZI GCGATG 2 cut(s) 1563, 1625
BtsCI GGATG 6 cut(s) 166, 379, 780, 1175, 1280, 1502
BtsIMutI CAGTG 3 cut(s) 24, 88, 1427
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 3 cut(s) 838, 1148, 1589
CfoI GCGC 1 cut(s) 1707
Cfr13I GGNCC 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 663
CseI GACGC 3 cut(s) 473, 838, 1344
Csp6I GTAC 4 cut(s) 72, 794, 1365, 1460
CviAII CATG 8 cut(s) 280, 652, 680, 773, 785, 1201, 1605, 1739
CviQI GTAC 4 cut(s) 72, 794, 1365, 1460
DdeI CTNAG 7 cut(s) 237, 265, 451, 510, 977, 1220, 1430
DpnI GATC 9 cut(s) 53, 80, 180, 662, 666, 721, 777, 966, 1214
DpnII GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
DraI TTTAAA 2 cut(s) 132, 358
DraIII CACNNNGTG 1 cut(s) 812
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 3 cut(s) 42, 889, 1724
Eam1104I CTCTTC 1 cut(s) 1179
EarI CTCTTC 1 cut(s) 1179
Eco105I TACGTA 1 cut(s) 1364
Eco130I CCWWGG 2 cut(s) 225, 651
Eco32I GATATC 2 cut(s) 31, 1295
Eco81I CCTNAGG 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 1555
EcoRV GATATC 2 cut(s) 31, 1295
EcoT14I CCWWGG 2 cut(s) 225, 651
EcoT22I ATGCAT 3 cut(s) 774, 1033, 1641
ErhI CCWWGG 2 cut(s) 225, 651
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 8 cut(s) 283, 655, 683, 776, 788, 1204, 1608, 1742
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 2 cut(s) 728, 1593
FatI CATG 8 cut(s) 279, 651, 679, 772, 784, 1200, 1604, 1738
FbaI TGATCA 2 cut(s) 964, 1212
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 3 cut(s) 377, 1139, 1334
FokI GGATG 6 cut(s) 173, 366, 767, 1182, 1287, 1509
Fsp4HI GCNGC 3 cut(s) 377, 1139, 1334
FspBI CTAG 3 cut(s) 36, 1095, 1194
GlaI GCGC 1 cut(s) 1706
GluI GCNGC 3 cut(s) 377, 1139, 1334
HaeIII GGCC 4 cut(s) 44, 224, 891, 1726
HgaI GACGC 3 cut(s) 473, 838, 1344
HhaI GCGC 1 cut(s) 1707
Hin1I GRCGYC 1 cut(s) 849
Hin1II CATG 8 cut(s) 283, 655, 683, 776, 788, 1204, 1608, 1742
Hin6I GCGC 1 cut(s) 1705
HinP1I GCGC 1 cut(s) 1705
HincII GTYRAC 3 cut(s) 213, 461, 1023
HindII GTYRAC 3 cut(s) 213, 461, 1023
HinfI GANTC 6 cut(s) 506, 830, 996, 1107, 1177, 1515
HpaI GTTAAC 1 cut(s) 1023
HphI GGTGA 5 cut(s) 259, 701, 824, 1102, 1414
Hpy166II GTNNAC 6 cut(s) 6, 22, 213, 461, 829, 1023
Hpy188I TCNGA 5 cut(s) 122, 511, 856, 1223, 1371
Hpy188III TCNNGA 3 cut(s) 993, 1181, 1259
Hpy8I GTNNAC 6 cut(s) 6, 22, 213, 461, 829, 1023
Hpy99I CGWCG 1 cut(s) 1360
HpyAV CCTTC 5 cut(s) 65, 80, 411, 982, 1754
HpyCH4III ACNGT 4 cut(s) 289, 826, 1415, 1617
HpyCH4IV ACGT 2 cut(s) 1325, 1363
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 609, 846, 1147, 1588
HpyF3I CTNAG 7 cut(s) 237, 265, 451, 510, 977, 1220, 1430
HpySE526I ACGT 2 cut(s) 1325, 1363
Hsp92I GRCGYC 1 cut(s) 849
Hsp92II CATG 8 cut(s) 283, 655, 683, 776, 788, 1204, 1608, 1742
HspAI GCGC 1 cut(s) 1705
Ksp22I TGATCA 2 cut(s) 964, 1212
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
LmnI GCTCC 2 cut(s) 536, 1440
Lsp1109I GCAGC 2 cut(s) 388, 1125
MaeI CTAG 3 cut(s) 36, 1095, 1194
MaeII ACGT 2 cut(s) 1325, 1363
MaeIII GTNAC 4 cut(s) 182, 247, 542, 1359
MalI GATC 9 cut(s) 53, 80, 180, 662, 666, 721, 777, 966, 1214
MboI GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
MboII GAAGA 4 cut(s) 188, 745, 1196, 1730
MfeI CAATTG 1 cut(s) 578
MflI RGATCY 2 cut(s) 51, 719
MhlI GDGCHC 1 cut(s) 287
MlsI TGGCCA 3 cut(s) 44, 891, 1726
MluNI TGGCCA 3 cut(s) 44, 891, 1726
MlyI GAGTC 2 cut(s) 824, 1524
Mox20I TGGCCA 3 cut(s) 44, 891, 1726
Mph1103I ATGCAT 3 cut(s) 774, 1033, 1641
MroXI GAANNNNTTC 1 cut(s) 1488
MscI TGGCCA 3 cut(s) 44, 891, 1726
MseI TTAA 7 cut(s) 131, 357, 645, 738, 1017, 1022, 1655
Msp20I TGGCCA 3 cut(s) 44, 891, 1726
MspCI CTTAAG 1 cut(s) 1016
MspR9I CCNGG 1 cut(s) 1557
MunI CAATTG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 1557
MvnI CGCG 3 cut(s) 1336, 1355, 1707
MwoI GCNNNNNNNGC 5 cut(s) 106, 609, 846, 1147, 1588
NcoI CCATGG 1 cut(s) 651
NdeII GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
NlaIII CATG 8 cut(s) 283, 655, 683, 776, 788, 1204, 1608, 1742
NlaIV GGNNCC 4 cut(s) 194, 223, 432, 1436
NmuCI GTSAC 2 cut(s) 247, 542
NsiI ATGCAT 3 cut(s) 774, 1033, 1641
PdmI GAANNNNTTC 1 cut(s) 1488
PfeI GAWTC 4 cut(s) 506, 996, 1107, 1177
PflMI CCANNNNNTGG 2 cut(s) 1265, 1444
PkrI GCNGC 3 cut(s) 378, 1140, 1335
PleI GAGTC 2 cut(s) 824, 1523
PpsI GAGTC 2 cut(s) 824, 1523
Ppu21I YACGTR 1 cut(s) 1364
PsiI TTATAA 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 1555
PspGI CCWGG 1 cut(s) 1555
PspN4I GGNNCC 4 cut(s) 194, 223, 432, 1436
PspPI GGNCC 1 cut(s) 222
PstI CTGCAG 1 cut(s) 1143
PsuI RGATCY 2 cut(s) 51, 719
RsaI GTAC 4 cut(s) 73, 795, 1366, 1461
RsaNI GTAC 4 cut(s) 72, 794, 1365, 1460
SaqAI TTAA 7 cut(s) 131, 357, 645, 738, 1017, 1022, 1655
SatI GCNGC 3 cut(s) 377, 1139, 1334
Sau3AI GATC 9 cut(s) 51, 78, 178, 660, 664, 719, 775, 964, 1212
Sau96I GGNCC 1 cut(s) 222
SchI GAGTC 2 cut(s) 824, 1524
ScrFI CCNGG 1 cut(s) 1557
SduI GDGCHC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 1139
SmlI CTYRAG 1 cut(s) 1016
SmoI CTYRAG 1 cut(s) 1016
SnaBI TACGTA 1 cut(s) 1364
SsiI CCGC 5 cut(s) 98, 423, 931, 1113, 1334
SspMI CTAG 3 cut(s) 36, 1095, 1194
StyD4I CCNGG 1 cut(s) 1555
StyI CCWWGG 2 cut(s) 225, 651
TaaI ACNGT 4 cut(s) 289, 826, 1415, 1617
TaiI ACGT 2 cut(s) 1328, 1366
TaqI TCGA 2 cut(s) 663, 1291
TatI WGTACW 1 cut(s) 793
TauI GCSGC 1 cut(s) 1336
TfiI GAWTC 4 cut(s) 506, 996, 1107, 1177
Tru1I TTAA 7 cut(s) 131, 357, 645, 738, 1017, 1022, 1655
Tru9I TTAA 7 cut(s) 131, 357, 645, 738, 1017, 1022, 1655
TscAI CASTG 3 cut(s) 24, 88, 1434
TseFI GTSAC 2 cut(s) 247, 542
TseI GCWGC 2 cut(s) 376, 1138
Tsp45I GTSAC 2 cut(s) 247, 542
TspDTI ATGAA 4 cut(s) 408, 774, 1116, 1593
TspGWI ACGGA 2 cut(s) 271, 1154
TspRI CASTG 3 cut(s) 24, 88, 1434
Van91I CCANNNNNTGG 2 cut(s) 1265, 1444
Vha464I CTTAAG 1 cut(s) 1016
XapI RAATTY 6 cut(s) 143, 301, 641, 761, 1152, 1540
XmiI GTMKAC 1 cut(s) 828
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 3 cut(s) 36, 1095, 1194
Zsp2I ATGCAT 3 cut(s) 774, 1033, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.