MD14G1068500.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001156
Physical Location & Seq
Reverse (-)
7460391 .. 7462516
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1068500.v1.1.491

Sequence Viewer

Length: 2073 bp
ATGACCGAGCTTGCTTTTCCGTTGGCAGCCAAACTCATTGAAAGGCTCAGCTCCATTGCTTCTGAGGAGATTTGCTTGGCGTGGGGTGTTAAAGCAGATCTGCAAAACCTTCGACGCACCATGTCCACAATCAAAGACGTGCTCTTGGATGCCGAAGAGAAGCAAGCACGTAACGATGATCTAAGCAGTTGGCTACGAGAACTTAAGGATGTGTTCCTTGATGCGGAGGATCTGTTGGACGAGTTTGAGTGCGAAGCTTTGCGAAGGCAAGTGGTTCATGGCAGTGGCATAACTAGAAAGGTACGCCGTTTCTTTTCCCGCTCTAATCCAGTTGCATTCGACTTGCGAGTAGGTCATGGAATTAAGGAAATACGAGCGAGGTTACAAGAGCTTAAAGAAGATAAGAATTTTGCTGATCTTCGACCATTTCATCATCACCCACATCTCGGTGATCATCTGAGGGAGAATAGGAATACGACCCACTCATTTGTCCGTGCTTCGGAGGTTATTGGTAGAGACACGGAGAAAAATACAGTTGTAGATCTTCTAATGCAACAAGGCGATGATCATCAAGGTGGGGATAATGGGAATGTATCTGTTATTCCTATAGTGGGATTTGGAGGTTTAGGGAAGACCACCCTTGCCCAGTCGGTGTACAATGATCAGAGAGTCGTTGGAAGTTTTGACTTAAGAATGTGGCAGCATGTGTCAGTGGACTTTGATGTTACTAGCTTGACAAAAGAGATCCTTGGCTCTGCATTAGGTACACAGATCAGTGAAGGGCTGTCTCTGGATCAGTTGCAAGCACAACTACGAGATGCTTTAAAGGATAAAAAATTTCTGCTTGTCCTGGATGATCTGTGGAACGTAGATCGTATCAAATGGAGCGAGTTGAGAGATTTGTTGGTAGAGGGGGCTAAGATAGGATCTAAGATTTTGGTGACAACACGGAATATCTCAATTGCTTCAATCATGGGCACAGTTGGAACATACATAAATTTAGAAGTTCTCACTTTTGAGGATTGTATGAAGTTGTTTGTGAAGTGTGCATTTGAAGAAGGACAAGAAAGACAACATCCAAGCCTCTATGAAATGGGAGAAGATATTGTAGGAAAGTGCGGAGGGGTTCCCTTAGCCGTGAAAACCTTAGGGAGTCAACTTTACTCAAAGACTGATGAGCGTCAATGGAAGTTGATTAGAGATTCGGAGATTTGGGAATTAGAACGAGGGGGAGATGGTCACATTCTACCTGCTTTGAGACTGAGTTATACTCAATTGCCCCCTCATTTGAAGCATTGCCTTGTTTGCTGTTCAACTCTTCCAAAGAATTACACTAAATTTGATAGCAGGGAATTAATCAATAATTGGATGGCACATGGAATCCTTGAATCTCGTGAACATGGGGATATGGAGTTGGAAGATGTTGGCGAGCTATATTTCAAAGAGTTATGGGACAGATCTTTCTTTCAAAATGTTAAGGATTACGCTTTCTTCTATGAATTTGATATGCATGATCTCATCCATGACCTTGTACAATCAGTCGGACAAGGTGAGAGTTTGATAGTAGACTCTGCAGGGACCAAAGGCATCTCTGAAAATGTTAGACATCTAGCAATTTTGAAAAGTGGCCAAAATGTTTCAACAACCTTGCAAAAGTTGAACAAAGTGCGGACCATAGGAGTAGTGGATTGTAAAGATATTGATGAATCCTTCCTCCGCACTTGCATTTCAAGATTCAAATATCTGCGGTGCCTTGGATTAGTCAATGCATCTTGGGAATTGTTGCCGAGTTCCATTGGTTCTTTGAAGCATTTGAGAAGCCTGAACTTGGCCGGAAATGTAAGAATCACCGCACTACCCAATTCAATTTGTAAGCTGCAGAGCCTGCAAGCTCTGACTCTCGGTAGATGCGTGAAACTTGAAGAGTTGCCTGGAGACATGAGCAAGTTGATCAGCCTTAGATGCCTCGAATTTACCACAAAACAATCAAGCTTTCCAGAGAATGGAGTGGGATGCTTGACATCACTTCGATATCTTTGTATTCAGGATTGTAGTTATCTAACCCAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

691

Amino Acids

77.95

Weight (kDa)

5.91

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 96 7.8e-25 Rx N-terminal domain
NB-ARC PF00931 195 - 353 3.9e-30 NB-ARC domain
WHD_DRP PF23559 441 - 511 2.9e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 534 - 682 7.5e-18 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1258
AccB1I GGYRCC 1 cut(s) 1749
AccB7I CCANNNNNTGG 1 cut(s) 2003
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 1566
AciI CCGC 7 cut(s) 224, 319, 1119, 1669, 1717, 1747, 1851
AclWI GGATC 4 cut(s) 237, 739, 801, 934
AcoI YGGCCR 2 cut(s) 1627, 1830
AcsI RAATTY 6 cut(s) 406, 836, 997, 1337, 1499, 1970
AfaI GTAC 4 cut(s) 303, 656, 766, 1533
AfiI CCNNNNNNNGG 6 cut(s) 223, 446, 499, 611, 1828, 2003
AflII CTTAAG 2 cut(s) 203, 688
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 849, 1930
AluBI AGCT 9 cut(s) 10, 51, 257, 391, 732, 1432, 1876, 1892, 1992
AluI AGCT 9 cut(s) 10, 51, 257, 391, 732, 1432, 1876, 1892, 1992
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 4 cut(s) 510, 792, 1252, 1929
AlwI GGATC 4 cut(s) 237, 739, 801, 934
AlwNI CAGNNNCTG 1 cut(s) 1885
AoxI GGCC 2 cut(s) 1627, 1830
ApeKI GCWGC 3 cut(s) 26, 700, 1876
ApoI RAATTY 6 cut(s) 406, 836, 997, 1337, 1499, 1970
AseI ATTAAT 1 cut(s) 1355
AspS9I GGNCC 2 cut(s) 1578, 1671
AsuHPI GGTGA 5 cut(s) 428, 461, 952, 1562, 1840
AvaII GGWCC 2 cut(s) 1578, 1671
AxyI CCTNAGG 1 cut(s) 1147
BaeGI GKGCMC 1 cut(s) 980
BalI TGGCCA 1 cut(s) 1629
BanI GGYRCC 1 cut(s) 1749
BauI CACGAG 1 cut(s) 1392
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 38, 712, 1863
BccI CCATC 2 cut(s) 1229, 1363
BceAI ACGGC 2 cut(s) 291, 1121
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 2 cut(s) 851, 1932
BclI TGATCA 4 cut(s) 451, 565, 661, 1950
BcoDI GTCTC 4 cut(s) 510, 792, 1252, 1929
BfaI CTAG 3 cut(s) 294, 729, 1610
BfmI CTRYAG 3 cut(s) 606, 1572, 1877
BfrI CTTAAG 2 cut(s) 203, 688
BfuAI ACCTGC 1 cut(s) 1258
BglII AGATCT 3 cut(s) 97, 541, 1457
BisI GCNGC 3 cut(s) 27, 701, 1877
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 3 cut(s) 28, 702, 1878
Bme1390I CCNGG 2 cut(s) 851, 1932
Bme18I GGWCC 2 cut(s) 1578, 1671
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 1578, 1671
BmiI GGNNCC 3 cut(s) 1128, 1579, 1751
BmrFI CCNGG 2 cut(s) 851, 1932
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 8 cut(s) 139, 211, 808, 1596, 1778, 1898, 1952, 2003
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 638
BplI GAGNNNNNCTC 2 cut(s) 1255, 1287
BpmI CTGGAG 1 cut(s) 1953
Bpu10I CCTNAGC 1 cut(s) 1132
Bpu1102I GCTNAGC 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 748, 1753
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 446, 499, 611, 1828, 2003
Bse1I ACTGG 2 cut(s) 329, 646
Bse21I CCTNAGG 1 cut(s) 1147
Bse3DI GCAATG 2 cut(s) 54, 1294
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 851, 1932
BseDI CCNNGG 2 cut(s) 748, 1753
BseGI GGATG 7 cut(s) 154, 214, 859, 1075, 1374, 1518, 2018
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 6 cut(s) 223, 446, 499, 611, 1828, 2003
BseMI GCAATG 2 cut(s) 54, 1294
BseMII CTCAG 4 cut(s) 54, 61, 449, 1253
BseNI ACTGG 2 cut(s) 329, 646
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 980
BseXI GCAGC 3 cut(s) 38, 712, 1863
BshFI GGCC 2 cut(s) 1629, 1832
BshNI GGYRCC 1 cut(s) 1749
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 1833
BslFI GGGAC 2 cut(s) 1466, 1591
BslI CCNNNNNNNGG 6 cut(s) 223, 446, 499, 611, 1828, 2003
BsmAI GTCTC 4 cut(s) 510, 792, 1252, 1929
BsmFI GGGAC 2 cut(s) 1466, 1591
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 2 cut(s) 1629, 1832
Bsp1286I GDGCHC 2 cut(s) 144, 980
Bsp1407I TGTACA 2 cut(s) 654, 1531
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 7 cut(s) 224, 319, 1119, 1669, 1717, 1747, 1851
BspANI GGCC 2 cut(s) 1629, 1832
BspCNI CTCAG 4 cut(s) 55, 60, 450, 1254
BspLI GGNNCC 3 cut(s) 1128, 1579, 1751
BspMAI CTGCAG 2 cut(s) 1576, 1881
BspMI ACCTGC 1 cut(s) 1258
BspPI GGATC 4 cut(s) 237, 739, 801, 934
BspT107I GGYRCC 1 cut(s) 1749
BspTI CTTAAG 2 cut(s) 203, 688
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 2 cut(s) 54, 1294
BsrGI TGTACA 2 cut(s) 654, 1531
BsrI ACTGG 2 cut(s) 329, 646
BssECI CCNNGG 2 cut(s) 748, 1753
BssSI CACGAG 1 cut(s) 1392
BssT1I CCWWGG 2 cut(s) 748, 1753
Bst2BI CACGAG 1 cut(s) 1392
Bst2UI CCWGG 2 cut(s) 851, 1932
Bst4CI ACNGT 2 cut(s) 535, 982
Bst6I CTCTTC 3 cut(s) 150, 1323, 1917
BstAFI CTTAAG 2 cut(s) 203, 688
BstAPI GCANNNNNTGC 1 cut(s) 1885
BstAUI TGTACA 2 cut(s) 654, 1531
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 6 cut(s) 12, 165, 804, 1430, 1886, 1890
BstF5I GGATG 7 cut(s) 154, 214, 859, 1075, 1374, 1518, 2018
BstMAI GTCTC 4 cut(s) 510, 792, 1252, 1929
BstMWI GCNNNNNNNGC 3 cut(s) 1305, 1885, 1962
BstNI CCWGG 2 cut(s) 851, 1932
BstNSI RCATGY 1 cut(s) 707
BstSCI CCNGG 2 cut(s) 849, 1930
BstSFI CTRYAG 3 cut(s) 606, 1572, 1877
BstSLI GKGCMC 1 cut(s) 980
BstV1I GCAGC 3 cut(s) 38, 712, 1863
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 6 cut(s) 97, 229, 541, 744, 926, 1457
BstYI RGATCY 6 cut(s) 97, 229, 541, 744, 926, 1457
Bsu36I CCTNAGG 1 cut(s) 1147
BsuRI GGCC 2 cut(s) 1629, 1832
BtgZI GCGATG 1 cut(s) 576
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 7 cut(s) 154, 214, 859, 1075, 1374, 1518, 2018
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 3 cut(s) 289, 717, 781
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 6 cut(s) 12, 165, 804, 1430, 1886, 1890
CaiI CAGNNNCTG 1 cut(s) 1885
Cfr13I GGNCC 2 cut(s) 1578, 1671
CseI GACGC 2 cut(s) 123, 1169
Csp6I GTAC 4 cut(s) 302, 655, 765, 1532
CviQI GTAC 4 cut(s) 302, 655, 765, 1532
DraI TTTAAA 1 cut(s) 825
EaeI YGGCCR 2 cut(s) 1627, 1830
Eam1104I CTCTTC 3 cut(s) 150, 1323, 1917
EarI CTCTTC 3 cut(s) 150, 1323, 1917
Eco130I CCWWGG 2 cut(s) 748, 1753
Eco32I GATATC 1 cut(s) 2033
Eco47I GGWCC 2 cut(s) 1578, 1671
Eco81I CCTNAGG 1 cut(s) 1147
EcoRII CCWGG 2 cut(s) 849, 1930
EcoRV GATATC 1 cut(s) 2033
EcoT14I CCWWGG 2 cut(s) 748, 1753
EcoT22I ATGCAT 2 cut(s) 1512, 1771
ErhI CCWWGG 2 cut(s) 748, 1753
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 2 cut(s) 1466, 1591
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 4 cut(s) 451, 565, 661, 1950
FblI GTMKAC 1 cut(s) 1566
Fnu4HI GCNGC 3 cut(s) 27, 701, 1877
FokI GGATG 7 cut(s) 161, 221, 866, 1062, 1381, 1505, 2025
Fsp4HI GCNGC 3 cut(s) 27, 701, 1877
FspBI CTAG 3 cut(s) 294, 729, 1610
GluI GCNGC 3 cut(s) 27, 701, 1877
GsuI CTGGAG 1 cut(s) 1953
HaeIII GGCC 2 cut(s) 1629, 1832
HapII CCGG 1 cut(s) 1833
HgaI GACGC 2 cut(s) 123, 1169
HincII GTYRAC 1 cut(s) 1157
HindII GTYRAC 1 cut(s) 1157
HindIII AAGCTT 2 cut(s) 255, 1990
HpaII CCGG 1 cut(s) 1833
HphI GGTGA 5 cut(s) 428, 461, 952, 1562, 1840
Hpy166II GTNNAC 7 cut(s) 126, 655, 715, 767, 1157, 1397, 1567
Hpy188I TCNGA 8 cut(s) 64, 459, 502, 666, 1207, 1544, 1594, 1896
Hpy188III TCNNGA 5 cut(s) 791, 1394, 1731, 1997, 2045
Hpy8I GTNNAC 7 cut(s) 126, 655, 715, 767, 1157, 1397, 1567
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 5 cut(s) 119, 258, 773, 1052, 1720
HpyCH4III ACNGT 2 cut(s) 535, 982
HpyCH4IV ACGT 3 cut(s) 138, 169, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 1305, 1885, 1962
HpySE526I ACGT 3 cut(s) 138, 169, 867
Ksp22I TGATCA 4 cut(s) 451, 565, 661, 1950
LmnI GCTCC 2 cut(s) 56, 885
Lsp1109I GCAGC 3 cut(s) 38, 712, 1863
LweI GCATC 8 cut(s) 139, 211, 808, 1596, 1778, 1898, 1952, 2003
MaeI CTAG 3 cut(s) 294, 729, 1610
MaeII ACGT 3 cut(s) 138, 169, 867
MaeIII GTNAC 5 cut(s) 170, 381, 724, 940, 1238
MbiI CCGCTC 1 cut(s) 321
MfeI CAATTG 3 cut(s) 960, 1274, 2066
MflI RGATCY 6 cut(s) 97, 229, 541, 744, 926, 1457
MhlI GDGCHC 2 cut(s) 144, 980
MlsI TGGCCA 1 cut(s) 1629
MluNI TGGCCA 1 cut(s) 1629
MlyI GAGTC 4 cut(s) 678, 1162, 1562, 1891
MmeI TCCRAC 5 cut(s) 216, 655, 964, 1395, 1522
Mox20I TGGCCA 1 cut(s) 1629
Mph1103I ATGCAT 2 cut(s) 1512, 1771
MscI TGGCCA 1 cut(s) 1629
MseI TTAA 8 cut(s) 90, 204, 363, 393, 689, 824, 1355, 1476
MslI CAYNNNNRTG 3 cut(s) 282, 447, 573
Msp20I TGGCCA 1 cut(s) 1629
MspCI CTTAAG 2 cut(s) 203, 688
MspI CCGG 1 cut(s) 1833
MspR9I CCNGG 2 cut(s) 851, 1932
MunI CAATTG 3 cut(s) 960, 1274, 2066
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 2 cut(s) 851, 1932
MwoI GCNNNNNNNGC 3 cut(s) 1305, 1885, 1962
NlaIV GGNNCC 3 cut(s) 1128, 1579, 1751
NmeAIII GCCGAG 1 cut(s) 1812
NmuCI GTSAC 2 cut(s) 940, 1238
NsiI ATGCAT 2 cut(s) 1512, 1771
NspI RCATGY 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 1908
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 6 cut(s) 1202, 1380, 1388, 1706, 1734, 1845
PflMI CCANNNNNTGG 1 cut(s) 2003
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 3 cut(s) 28, 702, 1878
PleI GAGTC 4 cut(s) 677, 1161, 1562, 1891
PpsI GAGTC 4 cut(s) 677, 1161, 1562, 1891
Ppu21I YACGTR 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 1355
Psp6I CCWGG 2 cut(s) 849, 1930
PspGI CCWGG 2 cut(s) 849, 1930
PspN4I GGNNCC 3 cut(s) 1128, 1579, 1751
PspPI GGNCC 2 cut(s) 1578, 1671
PstI CTGCAG 2 cut(s) 1576, 1881
PstNI CAGNNNCTG 1 cut(s) 1885
PsuI RGATCY 6 cut(s) 97, 229, 541, 744, 926, 1457
RsaI GTAC 4 cut(s) 303, 656, 766, 1533
RsaNI GTAC 4 cut(s) 302, 655, 765, 1532
RseI CAYNNNNRTG 3 cut(s) 282, 447, 573
SaqAI TTAA 8 cut(s) 90, 204, 363, 393, 689, 824, 1355, 1476
SatI GCNGC 3 cut(s) 27, 701, 1877
Sau96I GGNCC 2 cut(s) 1578, 1671
SchI GAGTC 4 cut(s) 678, 1162, 1562, 1891
ScrFI CCNGG 2 cut(s) 851, 1932
SduI GDGCHC 2 cut(s) 144, 980
SfaNI GCATC 8 cut(s) 139, 211, 808, 1596, 1778, 1898, 1952, 2003
SfcI CTRYAG 3 cut(s) 606, 1572, 1877
SinI GGWCC 2 cut(s) 1578, 1671
SmiMI CAYNNNNRTG 3 cut(s) 282, 447, 573
SmlI CTYRAG 2 cut(s) 203, 688
SmoI CTYRAG 2 cut(s) 203, 688
SsiI CCGC 7 cut(s) 224, 319, 1119, 1669, 1717, 1747, 1851
SspMI CTAG 3 cut(s) 294, 729, 1610
StyD4I CCNGG 2 cut(s) 849, 1930
StyI CCWWGG 2 cut(s) 748, 1753
TaaI ACNGT 2 cut(s) 535, 982
TaiI ACGT 3 cut(s) 141, 172, 870
TaqI TCGA 5 cut(s) 112, 339, 421, 1968, 2029
TaqII GACCGA 1 cut(s) 20
TatI WGTACW 2 cut(s) 654, 1531
TfiI GAWTC 6 cut(s) 1202, 1380, 1388, 1706, 1734, 1845
Tru1I TTAA 8 cut(s) 90, 204, 363, 393, 689, 824, 1355, 1476
Tru9I TTAA 8 cut(s) 90, 204, 363, 393, 689, 824, 1355, 1476
TscAI CASTG 3 cut(s) 289, 717, 781
TseFI GTSAC 2 cut(s) 940, 1238
TseI GCWGC 3 cut(s) 26, 700, 1876
Tsp45I GTSAC 2 cut(s) 940, 1238
TspDTI ATGAA 6 cut(s) 266, 419, 1043, 1104, 1512, 1719
TspGWI ACGGA 4 cut(s) 9, 482, 536, 964
TspRI CASTG 3 cut(s) 289, 717, 781
Van91I CCANNNNNTGG 1 cut(s) 2003
Vha464I CTTAAG 2 cut(s) 203, 688
VpaK11BI GGWCC 2 cut(s) 1578, 1671
VspI ATTAAT 1 cut(s) 1355
XapI RAATTY 6 cut(s) 406, 836, 997, 1337, 1499, 1970
XceI RCATGY 1 cut(s) 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 1681
XmiI GTMKAC 1 cut(s) 1566
XspI CTAG 3 cut(s) 294, 729, 1610
Zsp2I ATGCAT 2 cut(s) 1512, 1771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.