MD12G1180400.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001156
Physical Location & Seq
Reverse (-)
26030045 .. 26040842
10798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1180400.v1.1.491

Sequence Viewer

Length: 2535 bp
ATGTGGGATTCGCACTCCCAACAATCTTTTCCAGTCATAACCCTCACCGTTGGCTATTTAAACGCATATTCACGATCAATTTGTATATATCCGTACCTTCACTACGAACCACAATCCTTGTACTCAGCTGAAATGGCTGAACGTGCCTTTGATTTGGCAGTCAGACTCACGGAGAAGCTAGGCTCCCTTGCTTACGACGAGATTTGCTTGGCATGGGCGGTTCAATCCGATCTGAGAAAGCTTGAGGGCACCATGTCCACAATCAAAGGCGTGCTCTTGGATGCTGAAGAGAAGCAAGCGAATAACAAGGAGCTCCGCAGTTGGCTCAGACAAGTTAAAAATGTGTTTCTTGATGCCGAGGATGTGTTGGATGAGTTTGAGTGTGAAGCCCTGCGAAAGCAAGTGGTGAAAAAATTTCATGGTACATGTAGAAAGGTACGTCGTTTTTTCTCCCGGTCTAATCCTGTTGCATTCCGTTTGAGGGTAGCTCATGAAATCAAAGAGTTATGGGAGAGGTTAGAAGAGCTTAAAGCCAATAAGTCTATCTTTGATTCTCTCACTAGTCATAGTAAAGGTTACTATGATGACCATGATAGCAATCTGAAGCAGGTGATCAAGGCGAGAGAGACGCACTCTTTCGTCCGTGCTTCGGAGGTTGTTGGTAGAGAATTTGAGAAAGAAGAGCTAGTAGATCGTTTGATTGAGCAAGGTGACGATCATTTCTCAGTAATTCCTGTAGTTGGAATGGGAGGTATAGGCAAGACTACACTTGCTAAGTTGGTGTACAATGATACAAGAGTCATTAAGAATTTTGAATTGATGATTTGGCAGTATGTGTCAGTAGATTTTGATATTGCTAGATTGACAAAAGAGATTCTTAGTTCTGTATTAGGTACAAATATTAGTGGTGAATTGTCTATGAATCAGTTGCAAGAAAAACTACGAGATGCTTTGAAGGATAAAATTTTTTTACTTGTGAACGAAAATGCATATAAATGGGGTGAGTTGAAAGATTTGTTGGTAGAGGGGGCCAAATTAGGAAGTAAGGTTTTAGTGACAACCCGTAATGTTACAGTTGCTTCCATCATGGGAACTGTTCCGACAAACATTAATTTGAAAATTCTCTCCGAAGAGGATTGCATGTCCTTGTTTGTGAAATGTGCATTTAAAGGAGGAGAAGAGAGAGAACATCCAAACCTCTTTGAAATTGGGAAAGATATTGTGAGAAAGTGTGGAGGGGTCCCCTTAGCGGTGAAAACTTTAGGATGTCAACTTTCCTCAAAGACTGATGAACGGTATTGGGAAATGATAAGGGATTCTGCAATATGGGAATTAGAAAGAGAGGGAGAAGCTCACGTTTTACCTGCTTTGAGACTTAGTTATACTCATTTGCCTCCCCATTTGAAAAGATGTCTTGCTTATTGTTCACTTCTTCCAAGGACATACGAAGGGCTTCATAGCTTGCTATTGATCAAAATTTGGATAGCAAATGGAATCCTTGAATCTCATGATCATGGGAATCTGGAGTTGGAAGATGTTGGTGAGCTATATTTTAAAGAGTTATGGGAGAGGTCCTTCTTTCAAAATGTTCAAGATAATAGTCTATTCTGCAAATTTCGTATGCATGATCTTATCCATGACCTCGTACGATCAGTTGCACAAGATGAGTGCTTTGCAGTAGACTTTGAAGGCACCAAAGAAATCTCTGAAAATGTTAGACATTTGTCAGTATTCAGACCAGCACAAAATGTTCCATCAATCTTGGATAAGCGGAACAGGTTGCGATCTATAACTGCGGAAGATATCAATGAATCCTTCCTTCAGACTTGCTTTTCAAGATTCAAGTATTTGCGGATGCTTGTTCTTCATGCTACAACATTTGAAGCTTTTCCAAGTTCCATTGTTTCCTTGAAGCATATGAGATACCTGGACTTGACTGGAAATGAAAGAATCACGAAACTCCCTGATGCAGTTTGTAATTTGCAAAGCTTGGAAATTTTAAATGTTTTTGGCTGTGTGAATCTTGAAGAATTGCCTAGAGATATAAGCAAGTTGATCAGCCTTAGATGGCTTGAGGTAACTACAAAGCAAACTACTTTTCCAGACAATGGAGTGGGATGCATGAAATCACTTCGGTATCTTTGTATTACGGATTGTGGTAATCTAACCTCTTTGCCACGTGATATGAGTTATCTTGATGCATTACACACTCTAATTATAGGCAATTGTGAGCAGCTTGATTTGGAGAACGGAAACTATCAACTAATTCCATTGAGGCTCCGAAGATTGGCTATTGGTGGAGTACCAAGGATGGTGGCATTGCCTGAATGGTTGCAGGGAGCTGCTAACACCCTACGAGTCTTGTATATTTGGGGTTGTGAAAACTTGGATGCATTACCTGAGTGGTTGACAAGTTTCACATCCCTTAGAATACTTGACCTTTCAGGTTGTCCAAAATTGTTGGCTCTGCCAGAAGGGATGCATCCACTCGAAGTTAAAATAGAGGATTGTCCTCAATTGAAGAGGGGAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

845

Amino Acids

96.56

Weight (kDa)

6.0

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 56 - 141 1.8e-23 Rx N-terminal domain
NB-ARC PF00931 225 - 390 8.1e-28 NB-ARC domain
WHD_DRP PF23559 489 - 549 2e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 594 - 814 7e-24 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 2 cut(s) 598, 1372
AccB1I GGYRCC 2 cut(s) 248, 1691
AccB7I CCANNNNNTGG 1 cut(s) 2108
AccI GTMKAC 1 cut(s) 1680
AciI CCGC 6 cut(s) 218, 316, 1250, 1771, 1796, 1852
AcsI RAATTY 8 cut(s) 413, 668, 808, 963, 1119, 1476, 1613, 1995
AcuI CTGAAG 3 cut(s) 306, 623, 1805
AcvI CACGTG 1 cut(s) 2180
AfaI GTAC 8 cut(s) 95, 122, 424, 438, 785, 895, 1647, 2304
AfiI CCNNNNNNNGG 6 cut(s) 481, 649, 740, 1249, 2108, 2287
AflIII ACRYGT 1 cut(s) 425
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 1926
AjuI GAANNNNNNNTTGG 6 cut(s) 12, 44, 1001, 1033, 1511, 1543
Alw21I GWGCWC 2 cut(s) 276, 315
Alw26I GTCTC 2 cut(s) 620, 1366
AoxI GGCC 1 cut(s) 1029
ApeKI GCWGC 2 cut(s) 2233, 2342
ApoI RAATTY 8 cut(s) 413, 668, 808, 963, 1119, 1476, 1613, 1995
AseI ATTAAT 1 cut(s) 1110
Asp700I GAANNNNTTC 2 cut(s) 1452, 1887
AspS9I GGNCC 3 cut(s) 1029, 1240, 1572
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 8 cut(s) 37, 418, 622, 722, 920, 1013, 1264, 1553
AvaII GGWCC 2 cut(s) 1240, 1572
BaeGI GKGCMC 1 cut(s) 251
BaeI ACNNNNGTAYC 4 cut(s) 783, 816, 2120, 2153
BanI GGYRCC 2 cut(s) 248, 1691
BanII GRGCYC 1 cut(s) 315
BbrPI CACGTG 1 cut(s) 2180
Bbv12I GWGCWC 2 cut(s) 276, 315
BbvI GCAGC 2 cut(s) 2245, 2329
BccI CCATC 4 cut(s) 1091, 1762, 2061, 2305
BciT130I CCWGG 1 cut(s) 1928
BclI TGATCA 4 cut(s) 612, 1470, 1510, 2055
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 620, 1366
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 6 cut(s) 179, 561, 686, 858, 2037, 2533
BfmI CTRYAG 1 cut(s) 735
BfuAI ACCTGC 2 cut(s) 598, 1372
BisI GCNGC 2 cut(s) 2234, 2343
BlsI GCNGC 2 cut(s) 2235, 2344
Bme1390I CCNGG 2 cut(s) 454, 1928
Bme18I GGWCC 2 cut(s) 1240, 1572
BmgT120I GGNCC 3 cut(s) 1029, 1240, 1572
BmiI GGNNCC 7 cut(s) 184, 250, 1030, 1241, 1242, 1693, 2279
BmrFI CCNGG 2 cut(s) 454, 1928
BoxI GACNNNNGTC 1 cut(s) 1723
BplI GAGNNNNNCTC 4 cut(s) 472, 504, 617, 649
BpmI CTGGAG 1 cut(s) 1544
Bpu10I CCTNAGC 1 cut(s) 1246
BpuEI CTTGAG 2 cut(s) 263, 2093
BpuMI CCSGG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 2180
BsaBI GATNNNNATC 1 cut(s) 597
BsaJI CCNNGG 3 cut(s) 357, 1436, 2306
Bsc4I CCNNNNNNNGG 6 cut(s) 481, 649, 740, 1249, 2108, 2287
Bse1I ACTGG 2 cut(s) 32, 1942
Bse3DI GCAATG 1 cut(s) 2318
Bse8I GATNNNNATC 1 cut(s) 597
BseBI CCWGG 1 cut(s) 1928
BseDI CCNNGG 3 cut(s) 357, 1436, 2306
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 6 cut(s) 481, 649, 740, 1249, 2108, 2287
BseMI GCAATG 1 cut(s) 2318
BseMII CTCAG 5 cut(s) 138, 224, 340, 738, 2391
BseNI ACTGG 2 cut(s) 32, 1942
BseRI GAGGAG 1 cut(s) 1188
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 2 cut(s) 2245, 2329
BshFI GGCC 1 cut(s) 1031
BshNI GGYRCC 2 cut(s) 248, 1691
BsiHKAI GWGCWC 2 cut(s) 276, 315
BsiSI CCGG 1 cut(s) 454
BsiWI CGTACG 1 cut(s) 1645
BslFI GGGAC 1 cut(s) 1226
BslI CCNNNNNNNGG 6 cut(s) 481, 649, 740, 1249, 2108, 2287
BsmAI GTCTC 2 cut(s) 620, 1366
BsmBI CGTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 1226
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 1 cut(s) 1031
Bsp1286I GDGCHC 3 cut(s) 251, 276, 315
Bsp1407I TGTACA 1 cut(s) 783
BspACI CCGC 6 cut(s) 218, 316, 1250, 1771, 1796, 1852
BspANI GGCC 1 cut(s) 1031
BspCNI CTCAG 5 cut(s) 137, 225, 339, 737, 2392
BspHI TCATGA 2 cut(s) 490, 1507
BspLI GGNNCC 7 cut(s) 184, 250, 1030, 1241, 1242, 1693, 2279
BspMI ACCTGC 2 cut(s) 598, 1372
BspQI GCTCTTC 2 cut(s) 516, 675
BspT107I GGYRCC 2 cut(s) 248, 1691
BsrDI GCAATG 1 cut(s) 2318
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 2 cut(s) 32, 1942
BssECI CCNNGG 3 cut(s) 357, 1436, 2306
BssT1I CCWWGG 2 cut(s) 1436, 2306
Bst2UI CCWGG 1 cut(s) 1928
Bst4CI ACNGT 4 cut(s) 49, 1075, 1096, 1296
Bst6I CTCTTC 6 cut(s) 282, 516, 675, 1125, 1173, 2516
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 2180
BstC8I GCNNGC 3 cut(s) 272, 297, 1463
BstMAI GTCTC 2 cut(s) 620, 1366
BstMWI GCNNNNNNNGC 2 cut(s) 134, 143
BstNI CCWGG 1 cut(s) 1928
BstNSI RCATGY 2 cut(s) 429, 1144
BstPAI GACNNNNGTC 1 cut(s) 1723
BstSCI CCNGG 2 cut(s) 452, 1926
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 2245, 2329
BsuRI GGCC 1 cut(s) 1031
BveI ACCTGC 2 cut(s) 598, 1372
Cac8I GCNNGC 3 cut(s) 272, 297, 1463
CciI TCATGA 2 cut(s) 490, 1507
Cfr13I GGNCC 3 cut(s) 1029, 1240, 1572
CseI GACGC 1 cut(s) 637
Csp6I GTAC 8 cut(s) 94, 121, 423, 437, 784, 894, 1646, 2303
CspCI CAANNNNNGTGG 4 cut(s) 99, 134, 2295, 2330
CviQI GTAC 8 cut(s) 94, 121, 423, 437, 784, 894, 1646, 2303
DraI TTTAAA 4 cut(s) 60, 1168, 1555, 2001
Eam1104I CTCTTC 6 cut(s) 282, 516, 675, 1125, 1173, 2516
EarI CTCTTC 6 cut(s) 282, 516, 675, 1125, 1173, 2516
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 1436, 2306
Eco24I GRGCYC 1 cut(s) 315
Eco32I GATATC 1 cut(s) 1804
Eco47I GGWCC 2 cut(s) 1240, 1572
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 3 cut(s) 306, 623, 1805
Eco72I CACGTG 1 cut(s) 2180
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 1240, 1572
EcoRII CCWGG 1 cut(s) 1926
EcoRV GATATC 1 cut(s) 1804
EcoT14I CCWWGG 2 cut(s) 1436, 2306
EcoT22I ATGCAT 6 cut(s) 991, 1626, 2123, 2203, 2395, 2484
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 2 cut(s) 1436, 2306
Esp3I CGTCTC 1 cut(s) 620
FalI AAGNNNNNCTT 4 cut(s) 530, 562, 861, 893
FaqI GGGAC 1 cut(s) 1226
FauNDI CATATG 1 cut(s) 1917
FbaI TGATCA 4 cut(s) 612, 1470, 1510, 2055
FblI GTMKAC 1 cut(s) 1680
Fnu4HI GCNGC 2 cut(s) 2234, 2343
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 2 cut(s) 2234, 2343
FspBI CTAG 6 cut(s) 179, 561, 686, 858, 2037, 2533
GluI GCNGC 2 cut(s) 2234, 2343
GsuI CTGGAG 1 cut(s) 1544
HaeIII GGCC 1 cut(s) 1031
HapII CCGG 1 cut(s) 454
HgaI GACGC 1 cut(s) 637
HincII GTYRAC 2 cut(s) 1271, 2409
HindII GTYRAC 2 cut(s) 1271, 2409
HindIII AAGCTT 3 cut(s) 239, 1884, 1987
HpaII CCGG 1 cut(s) 454
HphI GGTGA 8 cut(s) 37, 418, 622, 722, 920, 1013, 1264, 1553
Hpy166II GTNNAC 7 cut(s) 258, 784, 979, 1271, 1427, 1681, 2409
Hpy8I GTNNAC 7 cut(s) 258, 784, 979, 1271, 1427, 1681, 2409
Hpy99I CGWCG 2 cut(s) 200, 444
HpyAV CCTTC 8 cut(s) 107, 949, 1442, 1585, 1682, 1825, 1829, 2468
HpyCH4III ACNGT 4 cut(s) 49, 1075, 1096, 1296
HpyCH4IV ACGT 4 cut(s) 142, 439, 1356, 2179
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 143
HpySE526I ACGT 4 cut(s) 142, 439, 1356, 2179
KflI GGGWCCC 1 cut(s) 1240
Ksp22I TGATCA 4 cut(s) 612, 1470, 1510, 2055
LguI GCTCTTC 2 cut(s) 516, 675
LmnI GCTCC 5 cut(s) 188, 310, 318, 2283, 2339
Lsp1109I GCAGC 2 cut(s) 2245, 2329
MaeI CTAG 6 cut(s) 179, 561, 686, 858, 2037, 2533
MaeII ACGT 4 cut(s) 142, 439, 1356, 2179
MaeIII GTNAC 5 cut(s) 575, 710, 1054, 1069, 2077
MfeI CAATTG 2 cut(s) 2224, 2516
MhlI GDGCHC 3 cut(s) 251, 276, 315
MlyI GAGTC 3 cut(s) 159, 807, 2367
MmeI TCCRAC 4 cut(s) 348, 721, 1124, 1509
Mph1103I ATGCAT 6 cut(s) 991, 1626, 2123, 2203, 2395, 2484
MroXI GAANNNNTTC 2 cut(s) 1452, 1887
MseI TTAA 9 cut(s) 59, 336, 528, 804, 1110, 1167, 1554, 2000, 2496
MslI CAYNNNNRTG 2 cut(s) 994, 1512
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 2 cut(s) 454, 1928
MunI CAATTG 2 cut(s) 2224, 2516
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 1928
MwoI GCNNNNNNNGC 2 cut(s) 134, 143
NciI CCSGG 1 cut(s) 454
NdeI CATATG 1 cut(s) 1917
NlaIV GGNNCC 7 cut(s) 184, 250, 1030, 1241, 1242, 1693, 2279
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 2 cut(s) 710, 1054
NsiI ATGCAT 6 cut(s) 991, 1626, 2123, 2203, 2395, 2484
NspI RCATGY 2 cut(s) 429, 1144
PagI TCATGA 2 cut(s) 490, 1507
PaqCI CACCTGC 1 cut(s) 598
PciI ACATGT 1 cut(s) 425
PciSI GCTCTTC 2 cut(s) 516, 675
PctI GAATGC 1 cut(s) 470
PdmI GAANNNNTTC 2 cut(s) 1452, 1887
Pfl23II CGTACG 1 cut(s) 1645
PflMI CCANNNNNTGG 1 cut(s) 2108
PkrI GCNGC 2 cut(s) 2235, 2344
PleI GAGTC 3 cut(s) 159, 806, 2366
PmaCI CACGTG 1 cut(s) 2180
PmlI CACGTG 1 cut(s) 2180
PpsI GAGTC 3 cut(s) 159, 806, 2366
Ppu21I YACGTR 1 cut(s) 2180
PpuMI RGGWCCY 2 cut(s) 1240, 1572
PscI ACATGT 1 cut(s) 425
PshAI GACNNNNGTC 1 cut(s) 1723
PshBI ATTAAT 1 cut(s) 1110
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 2 cut(s) 1240, 1572
Psp6I CCWGG 1 cut(s) 1926
PspCI CACGTG 1 cut(s) 2180
PspGI CCWGG 1 cut(s) 1926
PspLI CGTACG 1 cut(s) 1645
PspN4I GGNNCC 7 cut(s) 184, 250, 1030, 1241, 1242, 1693, 2279
PspPI GGNCC 3 cut(s) 1029, 1240, 1572
PspPPI RGGWCCY 2 cut(s) 1240, 1572
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 8 cut(s) 95, 122, 424, 438, 785, 895, 1647, 2304
RsaNI GTAC 8 cut(s) 94, 121, 423, 437, 784, 894, 1646, 2303
RseI CAYNNNNRTG 2 cut(s) 994, 1512
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 2 cut(s) 516, 675
SaqAI TTAA 9 cut(s) 59, 336, 528, 804, 1110, 1167, 1554, 2000, 2496
SatI GCNGC 2 cut(s) 2234, 2343
Sau96I GGNCC 3 cut(s) 1029, 1240, 1572
SchI GAGTC 3 cut(s) 159, 807, 2367
ScrFI CCNGG 2 cut(s) 454, 1928
SduI GDGCHC 3 cut(s) 251, 276, 315
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 2 cut(s) 1240, 1572
SmiMI CAYNNNNRTG 2 cut(s) 994, 1512
SmlI CTYRAG 2 cut(s) 242, 2072
SmoI CTYRAG 2 cut(s) 242, 2072
SpeI ACTAGT 1 cut(s) 560
SsiI CCGC 6 cut(s) 218, 316, 1250, 1771, 1796, 1852
SspI AATATT 1 cut(s) 901
SspMI CTAG 6 cut(s) 179, 561, 686, 858, 2037, 2533
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 452, 1926
StyI CCWWGG 2 cut(s) 1436, 2306
TaaI ACNGT 4 cut(s) 49, 1075, 1096, 1296
TaiI ACGT 4 cut(s) 145, 442, 1359, 2182
TaqI TCGA 1 cut(s) 2490
TatI WGTACW 2 cut(s) 120, 783
Tru1I TTAA 9 cut(s) 59, 336, 528, 804, 1110, 1167, 1554, 2000, 2496
Tru9I TTAA 9 cut(s) 59, 336, 528, 804, 1110, 1167, 1554, 2000, 2496
TseFI GTSAC 2 cut(s) 710, 1054
TseI GCWGC 2 cut(s) 2233, 2342
Tsp45I GTSAC 2 cut(s) 710, 1054
TspDTI ATGAA 9 cut(s) 407, 507, 935, 1305, 1445, 1824, 1856, 1959, 2138
TspGWI ACGGA 6 cut(s) 81, 185, 464, 632, 2165, 2265
Van91I CCANNNNNTGG 1 cut(s) 2108
VpaK11BI GGWCC 2 cut(s) 1240, 1572
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 8 cut(s) 413, 668, 808, 963, 1119, 1476, 1613, 1995
XceI RCATGY 2 cut(s) 429, 1144
XmiI GTMKAC 1 cut(s) 1680
XmnI GAANNNNTTC 2 cut(s) 1452, 1887
XspI CTAG 6 cut(s) 179, 561, 686, 858, 2037, 2533
Zsp2I ATGCAT 6 cut(s) 991, 1626, 2123, 2203, 2395, 2484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.