Rroxscaffold_6G00399360

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001156
Physical Location & Seq
Reverse (-)
21291043 .. 21292903
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399360.1

Sequence Viewer

Length: 843 bp
ATGGCTCATGGAATCCTTCAATCTTGTGATAATGGGAATCTGGAGATGGAAGATGTTGGCGAGCAATATTTTAAAGAGTTAATGGCGAGATCGTTCTTTGAAAATGTGGACATTAGTGATTTTGGCCAAGATGGCTACCATAATTTTTCTATGCATGATCTTATCTATGACCTTGAACAGTCAATTGGGCAAGATGAGTGGTCTGTTGTGGATTCTAGCACTAAAGACATCGTTGAAAATCAACTCCATCTGGTGAGCCAACACTATGAAACAATTCCATTGAGGCTCGAAAAGTTGGTTATTGTTGGACTACCACGGATCTGGGAGTTGCCTGATTGGTTTCGAGGAGCTGCCAACACCCTACAATACTTGTACATTGGAAATTGTTCCAATTTGGGTTCATTACCTGGGTGGCTCACAAATCTAACATCACTTAAAAAGCTTGTCCTCAAGAGTTGCCCAAAATTGTTGTCACTTCCAGAAGGGATGGATCGTCTCACTGCCTTGAGATACTTGAAGATGGAAGATTGTCCTGAGTTGCAGAGAAGATGCAAGTGTGACGCTGGTGAAGATTGGCCAAAGATTGCTCTGCTTACAGCGGAGGACAGCGGAGAGCAAGTGCAGACTGGATTCGATCCGTGCAGTGGAAGGCAGTTTGAGGTGCTGATCGGTCACATTACTTCACCGACAGTTGGGATCAGCAAGCGGCAGCGTGACGGGCTTGGAGGCACGATGGTGGTAGCTGGGCTGTCTGTGCGGCACATGTCGTGGGCCGAGCTCAATGGCTGGAGCTCTTTGGCCCTAGTTCAGTGGTGGCTTGATCTGGGCCTGGGGTTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

280

Amino Acids

31.59

Weight (kDa)

4.82

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 58 2.5e-12 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 599, 609, 706, 757
AclWI GGATC 4 cut(s) 326, 498, 629, 704
AcoI YGGCCR 2 cut(s) 124, 575
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 644, 692
AflIII ACRYGT 1 cut(s) 762
AgsI TTSAA 5 cut(s) 20, 101, 176, 236, 517
AjnI CCWGG 2 cut(s) 406, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AleI CACNNNNGTG 1 cut(s) 734
AluBI AGCT 5 cut(s) 350, 442, 743, 778, 792
AluI AGCT 5 cut(s) 350, 442, 743, 778, 792
Alw21I GWGCWC 2 cut(s) 780, 794
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 4 cut(s) 326, 498, 629, 704
AoxI GGCC 5 cut(s) 124, 575, 771, 798, 826
ApeKI GCWGC 2 cut(s) 350, 709
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
Asp700I GAANNNNTTC 2 cut(s) 273, 385
AspS9I GGNCC 3 cut(s) 771, 799, 826
AsuHPI GGTGA 3 cut(s) 265, 578, 675
BalI TGGCCA 2 cut(s) 126, 577
BanII GRGCYC 2 cut(s) 780, 794
Bbv12I GWGCWC 2 cut(s) 780, 794
BbvI GCAGC 2 cut(s) 337, 721
BccI CCATC 6 cut(s) 40, 125, 255, 481, 514, 727
BciT130I CCWGG 2 cut(s) 408, 830
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 2 cut(s) 216, 803
BglI GCCNNNNNGGC 1 cut(s) 132
BisI GCNGC 4 cut(s) 351, 707, 710, 758
BlsI GCNGC 4 cut(s) 352, 708, 711, 759
Bme1390I CCNGG 2 cut(s) 408, 830
BmgT120I GGNCC 3 cut(s) 771, 799, 826
BmrFI CCNGG 2 cut(s) 408, 830
BmsI GCATC 1 cut(s) 539
BpmI CTGGAG 2 cut(s) 62, 808
BpuEI CTTGAG 2 cut(s) 434, 526
BsaJI CCNNGG 3 cut(s) 314, 407, 829
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 644, 692
Bse1I ACTGG 1 cut(s) 631
BseBI CCWGG 2 cut(s) 408, 830
BseDI CCNNGG 3 cut(s) 314, 407, 829
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 2 cut(s) 644, 692
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 1 cut(s) 360
BseXI GCAGC 2 cut(s) 337, 721
BseYI CCCAGC 1 cut(s) 743
BsgI GTGCAG 2 cut(s) 641, 661
BshFI GGCC 5 cut(s) 126, 577, 773, 800, 828
BsiHKAI GWGCWC 2 cut(s) 780, 794
BslI CCNNNNNNNGG 2 cut(s) 644, 692
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsnI GGCC 5 cut(s) 126, 577, 773, 800, 828
Bsp1286I GDGCHC 2 cut(s) 780, 794
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
BspACI CCGC 4 cut(s) 599, 609, 706, 757
BspANI GGCC 5 cut(s) 126, 577, 773, 800, 828
BspCNI CTCAG 1 cut(s) 526
BspPI GGATC 4 cut(s) 326, 498, 629, 704
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 3 cut(s) 314, 407, 829
BssMI GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
Bst2UI CCWGG 2 cut(s) 408, 830
Bst4CI ACNGT 2 cut(s) 180, 691
BstAUI TGTACA 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 62, 704
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 492
BstKTI GATC 8 cut(s) 92, 160, 321, 493, 637, 669, 699, 823
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
BstMWI GCNNNNNNNGC 3 cut(s) 132, 718, 754
BstNI CCWGG 2 cut(s) 408, 830
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 2 cut(s) 406, 828
BstV1I GCAGC 2 cut(s) 337, 721
BstX2I RGATCY 1 cut(s) 318
BstXI CCANNNNNNTGG 1 cut(s) 321
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 5 cut(s) 126, 577, 773, 800, 828
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 492
BtsI GCAGTG 2 cut(s) 498, 649
BtsIMutI CAGTG 3 cut(s) 498, 649, 815
Cac8I GCNNGC 2 cut(s) 62, 704
Cfr13I GGNCC 3 cut(s) 771, 799, 826
CseI GACGC 1 cut(s) 569
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 179, 214
CviAII CATG 3 cut(s) 8, 155, 763
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 1 cut(s) 534
DpnI GATC 8 cut(s) 91, 159, 320, 492, 636, 668, 698, 822
DpnII GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
DraI TTTAAA 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 124, 575
Ecl136II GAGCTC 2 cut(s) 778, 792
Eco24I GRGCYC 2 cut(s) 780, 794
Eco53kI GAGCTC 2 cut(s) 778, 792
EcoICRI GAGCTC 2 cut(s) 778, 792
EcoRII CCWGG 2 cut(s) 406, 828
EcoT22I ATGCAT 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 780, 794
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 3 cut(s) 11, 158, 766
FaiI YATR 7 cut(s) 9, 141, 152, 156, 168, 267, 764
FatI CATG 3 cut(s) 7, 154, 762
Fnu4HI GCNGC 4 cut(s) 351, 707, 710, 758
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 2 cut(s) 780, 794
Fsp4HI GCNGC 4 cut(s) 351, 707, 710, 758
FspBI CTAG 2 cut(s) 216, 803
GluI GCNGC 4 cut(s) 351, 707, 710, 758
GsaI CCCAGC 1 cut(s) 747
GsuI CTGGAG 2 cut(s) 62, 808
HaeIII GGCC 5 cut(s) 126, 577, 773, 800, 828
HgaI GACGC 1 cut(s) 569
Hin1II CATG 3 cut(s) 11, 158, 766
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 12, 37, 212, 630
HphI GGTGA 3 cut(s) 265, 578, 675
Hpy166II GTNNAC 1 cut(s) 109
Hpy188III TCNNGA 4 cut(s) 41, 451, 479, 533
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 3 cut(s) 26, 476, 642
HpyCH4III ACNGT 2 cut(s) 180, 691
HpyCH4V TGCA 5 cut(s) 154, 541, 552, 622, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 718, 754
HpyF3I CTNAG 1 cut(s) 534
Hsp92II CATG 3 cut(s) 11, 158, 766
Kzo9I GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
LmnI GCTCC 2 cut(s) 347, 789
Lsp1109I GCAGC 2 cut(s) 337, 721
LweI GCATC 1 cut(s) 539
MaeI CTAG 2 cut(s) 216, 803
MaeIII GTNAC 4 cut(s) 471, 557, 671, 713
MalI GATC 8 cut(s) 91, 159, 320, 492, 636, 668, 698, 822
MboI GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
MboII GAAGA 5 cut(s) 62, 529, 536, 558, 581
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 780, 794
MlsI TGGCCA 2 cut(s) 126, 577
MluCI AATT 6 cut(s) 142, 183, 273, 382, 391, 464
MluNI TGGCCA 2 cut(s) 126, 577
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 6 cut(s) 276, 338, 458, 595, 652, 719
Mox20I TGGCCA 2 cut(s) 126, 577
Mph1103I ATGCAT 1 cut(s) 156
MroXI GAANNNNTTC 2 cut(s) 273, 385
MscI TGGCCA 2 cut(s) 126, 577
MseI TTAA 3 cut(s) 72, 80, 435
MslI CAYNNNNRTG 1 cut(s) 734
Msp20I TGGCCA 2 cut(s) 126, 577
MspA1I CMGCKG 2 cut(s) 599, 609
MspR9I CCNGG 2 cut(s) 408, 830
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 2 cut(s) 408, 830
MwoI GCNNNNNNNGC 3 cut(s) 132, 718, 754
NdeII GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
NlaIII CATG 3 cut(s) 11, 158, 766
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 4 cut(s) 471, 557, 671, 713
NsiI ATGCAT 1 cut(s) 156
NspI RCATGY 1 cut(s) 766
OliI CACNNNNGTG 1 cut(s) 734
PciI ACATGT 1 cut(s) 762
PdmI GAANNNNTTC 2 cut(s) 273, 385
PfeI GAWTC 4 cut(s) 12, 37, 212, 630
PkrI GCNGC 4 cut(s) 352, 708, 711, 759
PscI ACATGT 1 cut(s) 762
Psp124BI GAGCTC 2 cut(s) 780, 794
Psp6I CCWGG 2 cut(s) 406, 828
PspFI CCCAGC 1 cut(s) 743
PspGI CCWGG 2 cut(s) 406, 828
PspPI GGNCC 3 cut(s) 771, 799, 826
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 734
SacI GAGCTC 2 cut(s) 780, 794
SaqAI TTAA 3 cut(s) 72, 80, 435
SatI GCNGC 4 cut(s) 351, 707, 710, 758
Sau3AI GATC 8 cut(s) 89, 157, 318, 490, 634, 666, 696, 820
Sau96I GGNCC 3 cut(s) 771, 799, 826
ScrFI CCNGG 2 cut(s) 408, 830
SduI GDGCHC 2 cut(s) 780, 794
SetI ASST 8 cut(s) 174, 352, 409, 444, 663, 745, 780, 794
SfaNI GCATC 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 734
SmlI CTYRAG 2 cut(s) 449, 505
SmoI CTYRAG 2 cut(s) 449, 505
Sse9I AATT 6 cut(s) 142, 183, 273, 382, 391, 464
SsiI CCGC 4 cut(s) 599, 609, 706, 757
SspI AATATT 1 cut(s) 68
SspMI CTAG 2 cut(s) 216, 803
SstI GAGCTC 2 cut(s) 780, 794
StyD4I CCNGG 2 cut(s) 406, 828
TaaI ACNGT 2 cut(s) 180, 691
TaqI TCGA 3 cut(s) 288, 343, 633
TaqII GACCGA 1 cut(s) 659
TasI AATT 6 cut(s) 142, 183, 273, 382, 391, 464
TatI WGTACW 1 cut(s) 372
TauI GCSGC 2 cut(s) 709, 760
TfiI GAWTC 4 cut(s) 12, 37, 212, 630
Tru1I TTAA 3 cut(s) 72, 80, 435
Tru9I TTAA 3 cut(s) 72, 80, 435
TscAI CASTG 3 cut(s) 505, 649, 815
TseFI GTSAC 4 cut(s) 471, 557, 671, 713
TseI GCWGC 2 cut(s) 350, 709
Tsp45I GTSAC 4 cut(s) 471, 557, 671, 713
TspDTI ATGAA 2 cut(s) 282, 390
TspGWI ACGGA 2 cut(s) 331, 627
TspRI CASTG 3 cut(s) 505, 649, 815
XceI RCATGY 1 cut(s) 766
XmnI GAANNNNTTC 2 cut(s) 273, 385
XspI CTAG 2 cut(s) 216, 803
Zsp2I ATGCAT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.