MD14G1068900.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001156
Physical Location & Seq
Reverse (-)
7472780 .. 7474998
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1068900.v1.1.491

Sequence Viewer

Length: 2115 bp
ATGGCTAACCAGGCATTTCCGTTAGCAAGCAGACTCATTGAAAAGCTCGCCTCCATTGCTTCTGAGGAGATTTGCTTGGCGTGGGGTGTTAAAGTGGATCTGCAGAACCTTGGACGCACTATGTCCACAATCAGAGACGTGCTCTTGGATGCCGAAGAGAAGCAAGCACGTAAGAATGACCTACGCAGTTGGCTACGACAACTTAAAGATGTGTTCCTTGATGCGGAGGATCTGTTGGACGAGTTTGAATGTGAAGCTTTGCGAAGGCAAGTGGTTCGTGGCAGTGGCACAACTAGAAAGGTACACCGTTTCTTTTCCCGCTCTAATCCAGTTGCATTCCGCTTACGAGTAGGCCATGGAATTAAGGACATAAGAGAGAGGTTACAAAAGCTTAAAGATGATAAGAATTTTGCTGATCTTCGCACTGTTCATCATCACCCTCATCTAGGTGATCATGTGAGGGAGAACATGAATACGACCCACTCCTTTGTTCGTGCTTCGGAGGTTATTGGTAGAGAAACAGAAAAAAATAAAATTGTTGATCTTCTAATAATACAAGGCGATGATCATATAGGTGGGGATAATGGGAATGTTTCTGTTATTCCTATAGTGGGATTAGGAGGTTTAGGGAAGACCACCCTTGCCCAGTCAGTGTACAATGATCAGAGAGTTGTTGGGAGTTTTGACTTAAGAATGTGGCAGCATGTGTCAGTGGACTTTGATGTTACTAGATTGACAAAAGAGATCCTTGGCTCTGCATTAGGTACAAAGATCAGTGAAGGGTTGTCTCTGGATCAGTTGCAAGAACAACTACGAGACGCTTTAAAGGAAAAGAAATTTCTGCTTGTCTTGGATGATGTGTGGAATGAAGATCGTATCAAATGGAGCGAGTTGAGAGATTTGTTGATAGAGGGAGCCAAGGTGGGAACTAAGATTTTGGTGACGACACGGAGTATCTCAGTTGCTTCAATCATGGGGGCGGTTGGAAACATAAATTTAGACGTTCTCTCTTTTGAGGATTGTTTGTCTCTGTTTGTAAAGTGTGCATTTAAAGAAGGACAGGAAAGACAGCATCCAAGCCTGTATGAAATGGGAAAAGATATTGTAAGAAAGTGCGGAGGGGTTCCCTTAGCTGTGAAAACCTTAGGGAGTCAACTTTACTCAAAGACTGATGAGCGTCAATGGAAATTGATTAGAGATTCTGAGATATGGGAATTAGAACGAGATGGAGCTGGTCACATTCTACCTGCTTTGAGACTGAGTTATAATCAATTGCCATCTCATTTGAAGCAATGCCTTGTTTGCTGTTCAATTCTTCCAAAGAATTACATTAAATTTTATAGCAGCGAATTGATCAATCATTGGATGGCACATGGACTCCTTGAATCTCGTGATCATGGGAATATGGAGTGGGAAGATGTTGGTGAGCTATATTTCAAAGAGTTATGGGACAGATCTTTCTTTCAAAATGTTAAAGATTACACTTTCGGCTATGGATTTGATATGCATGATCTCATCAAGGACCTTGTACAATCAGTCGCACAAGGTGAGAGTTTGATAGTAGACTCTGCAGGGACCAAAGGCATCTCTGAAAATGTTAGACACCTAACAATTTTGAAAAGTGGCCAAAATGTATCAACAACCTTGCAAAAGTTGAATAAAGTGCGGACCATAGCACTAGAGAGTTGTGAAAATATTGATGAATCCTTCCTCAGCACTTGCATTTCAAGATTCAAATATCTGCGACTGCTTAAGTTAACCAATGCATCTTGGGAATTGTTGCCCAGTTCCATTGTTTCTTTGAAGCATTTGAGAAGCCTAGACTTGACCGCAAATGAAAGAATCACCGCACTACCCAATTCAATTTGTAAGCTGCAGAGCTTGCAAACTCTGAATCTCTGTGGATGCGTGAATCTTGAAGAGTTCCCTAGAGACATGAGAAACTTGATCAGCCTCAGATGGCTTGAATTAACCACAAAACAATCAAGTTTTCCAGAGAATGGAGTGGGATGCTTGACATCACTTCGATTTCTTTCTATTTGGGAGTGCAGTAATTCCACTAAGGCTCCAAAAATTGGGGATTCGGAAAGTACCACGAATGGTGGCATTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

705

Amino Acids

79.58

Weight (kDa)

6.7

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 98 6.6e-25 Rx N-terminal domain
NB-ARC PF00931 195 - 352 5.3e-31 NB-ARC domain
WHD_DRP PF23559 438 - 510 2e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 533 - 682 2.8e-19 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1266
Acc36I ACCTGC 1 cut(s) 1255
AccB7I CCANNNNNTGG 2 cut(s) 2000, 2075
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 1563
AciI CCGC 8 cut(s) 224, 319, 340, 980, 1116, 1666, 1830, 1848
AclWI GGATC 4 cut(s) 105, 237, 739, 801
AcoI YGGCCR 1 cut(s) 1624
AcsI RAATTY 4 cut(s) 406, 836, 994, 1334
AdeI CACNNNGTG 1 cut(s) 1547
AfaI GTAC 5 cut(s) 303, 656, 766, 1530, 2092
AfiI CCNNNNNNNGG 5 cut(s) 223, 446, 611, 2000, 2075
AflII CTTAAG 2 cut(s) 688, 1751
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 8 cut(s) 46, 257, 391, 1133, 1232, 1429, 1873, 1881
AluI AGCT 8 cut(s) 46, 257, 391, 1133, 1232, 1429, 1873, 1881
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 6 cut(s) 129, 792, 810, 1032, 1249, 1926
AlwI GGATC 4 cut(s) 105, 237, 739, 801
AoxI GGCC 2 cut(s) 352, 1624
ApeKI GCWGC 3 cut(s) 700, 1344, 1873
ApoI RAATTY 4 cut(s) 406, 836, 994, 1334
AspS9I GGNCC 3 cut(s) 1522, 1575, 1668
AsuHPI GGTGA 6 cut(s) 428, 461, 952, 1436, 1559, 1837
AvaII GGWCC 3 cut(s) 1522, 1575, 1668
AxyI CCTNAGG 1 cut(s) 1144
BalI TGGCCA 1 cut(s) 1626
BauI CACGAG 1 cut(s) 1389
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 1712
BbvI GCAGC 3 cut(s) 712, 1356, 1860
BccI CCATC 4 cut(s) 1220, 1285, 1360, 1953
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 6 cut(s) 451, 565, 661, 1353, 1393, 1947
BcoDI GTCTC 6 cut(s) 129, 792, 810, 1032, 1249, 1926
BfaI CTAG 6 cut(s) 294, 446, 729, 1679, 1820, 1929
BfmI CTRYAG 4 cut(s) 101, 606, 1569, 1874
BfrI CTTAAG 2 cut(s) 688, 1751
BfuAI ACCTGC 1 cut(s) 1255
BglII AGATCT 1 cut(s) 1454
BisI GCNGC 3 cut(s) 701, 1345, 1874
BlsI GCNGC 3 cut(s) 702, 1346, 1875
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 3 cut(s) 1522, 1575, 1668
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 1522, 1575, 1668
BmiI GGNNCC 4 cut(s) 916, 1125, 1576, 2067
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 2 cut(s) 640, 1779
BmsI GCATC 7 cut(s) 139, 211, 1081, 1593, 1775, 1895, 2000
BmuI ACTGGG 2 cut(s) 640, 1779
BpiI GAAGAC 1 cut(s) 638
BplI GAGNNNNNCTC 2 cut(s) 126, 158
Bpu10I CCTNAGC 2 cut(s) 1129, 1712
BsaAI YACGTR 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 109, 355, 748, 918
BsaXI ACNNNNNCTCC 8 cut(s) 218, 248, 906, 936, 1362, 1392, 2050, 2080
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 446, 611, 2000, 2075
Bse1I ACTGG 3 cut(s) 329, 646, 1785
Bse21I CCTNAGG 1 cut(s) 1144
Bse3DI GCAATG 3 cut(s) 54, 1298, 2106
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 4 cut(s) 109, 355, 748, 918
BseGI GGATG 6 cut(s) 154, 859, 1072, 1371, 1910, 2015
BseLI CCNNNNNNNGG 5 cut(s) 223, 446, 611, 2000, 2075
BseMI GCAATG 3 cut(s) 54, 1298, 2106
BseMII CTCAG 6 cut(s) 54, 972, 1194, 1250, 1726, 1969
BseNI ACTGG 3 cut(s) 329, 646, 1785
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 712, 1356, 1860
BsgI GTGCAG 1 cut(s) 2068
BshFI GGCC 2 cut(s) 354, 1626
BsiHKAI GWGCWC 1 cut(s) 144
BslFI GGGAC 2 cut(s) 1463, 1588
BslI CCNNNNNNNGG 5 cut(s) 223, 446, 611, 2000, 2075
BsmAI GTCTC 6 cut(s) 129, 792, 810, 1032, 1249, 1926
BsmBI CGTCTC 2 cut(s) 129, 810
BsmFI GGGAC 2 cut(s) 1463, 1588
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 2 cut(s) 354, 1626
Bsp1286I GDGCHC 1 cut(s) 144
Bsp1407I TGTACA 2 cut(s) 654, 1528
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 8 cut(s) 224, 319, 340, 980, 1116, 1666, 1830, 1848
BspANI GGCC 2 cut(s) 354, 1626
BspCNI CTCAG 6 cut(s) 55, 971, 1195, 1251, 1725, 1968
BspLI GGNNCC 4 cut(s) 916, 1125, 1576, 2067
BspMAI CTGCAG 3 cut(s) 105, 1573, 1878
BspMI ACCTGC 1 cut(s) 1255
BspPI GGATC 4 cut(s) 105, 237, 739, 801
BspTI CTTAAG 2 cut(s) 688, 1751
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 3 cut(s) 54, 1298, 2106
BsrGI TGTACA 2 cut(s) 654, 1528
BsrI ACTGG 3 cut(s) 329, 646, 1785
BssECI CCNNGG 4 cut(s) 109, 355, 748, 918
BssSI CACGAG 1 cut(s) 1389
BssT1I CCWWGG 4 cut(s) 109, 355, 748, 918
Bst2BI CACGAG 1 cut(s) 1389
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 308, 427
Bst6I CTCTTC 2 cut(s) 150, 1914
BstAFI CTTAAG 2 cut(s) 688, 1751
BstAPI GCANNNNNTGC 1 cut(s) 1882
BstAUI TGTACA 2 cut(s) 654, 1528
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 4 cut(s) 28, 48, 165, 1883
BstDSI CCRYGG 1 cut(s) 355
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 6 cut(s) 154, 859, 1072, 1371, 1910, 2015
BstMAI GTCTC 6 cut(s) 129, 792, 810, 1032, 1249, 1926
BstMWI GCNNNNNNNGC 4 cut(s) 11, 56, 1302, 1882
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 707
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 4 cut(s) 101, 606, 1569, 1874
BstV1I GCAGC 3 cut(s) 712, 1356, 1860
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 4 cut(s) 97, 229, 744, 1454
BstYI RGATCY 4 cut(s) 97, 229, 744, 1454
Bsu36I CCTNAGG 1 cut(s) 1144
BsuRI GGCC 2 cut(s) 354, 1626
BtgI CCRYGG 1 cut(s) 355
BtgZI GCGATG 1 cut(s) 576
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 6 cut(s) 154, 859, 1072, 1371, 1910, 2015
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 5 cut(s) 289, 423, 657, 717, 781
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 4 cut(s) 28, 48, 165, 1883
Cfr13I GGNCC 3 cut(s) 1522, 1575, 1668
CseI GACGC 3 cut(s) 123, 827, 1166
Csp6I GTAC 5 cut(s) 302, 655, 765, 1529, 2091
CviAII CATG 9 cut(s) 356, 455, 469, 704, 973, 1373, 1397, 1508, 1936
CviQI GTAC 5 cut(s) 302, 655, 765, 1529, 2091
DraI TTTAAA 2 cut(s) 825, 1051
DraIII CACNNNGTG 1 cut(s) 1547
EaeI YGGCCR 1 cut(s) 1624
Eam1104I CTCTTC 2 cut(s) 150, 1914
EarI CTCTTC 2 cut(s) 150, 1914
Eco130I CCWWGG 4 cut(s) 109, 355, 748, 918
Eco47I GGWCC 3 cut(s) 1522, 1575, 1668
Eco81I CCTNAGG 1 cut(s) 1144
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 1522
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 4 cut(s) 109, 355, 748, 918
EcoT22I ATGCAT 2 cut(s) 1509, 1768
ErhI CCWWGG 4 cut(s) 109, 355, 748, 918
Esp3I CGTCTC 2 cut(s) 129, 810
FaeI CATG 9 cut(s) 359, 458, 472, 707, 976, 1376, 1400, 1511, 1939
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FaqI GGGAC 2 cut(s) 1463, 1588
FatI CATG 9 cut(s) 355, 454, 468, 703, 972, 1372, 1396, 1507, 1935
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 6 cut(s) 451, 565, 661, 1353, 1393, 1947
FblI GTMKAC 1 cut(s) 1563
Fnu4HI GCNGC 3 cut(s) 701, 1345, 1874
FokI GGATG 6 cut(s) 161, 866, 1059, 1378, 1917, 2022
Fsp4HI GCNGC 3 cut(s) 701, 1345, 1874
FspBI CTAG 6 cut(s) 294, 446, 729, 1679, 1820, 1929
GluI GCNGC 3 cut(s) 701, 1345, 1874
HaeIII GGCC 2 cut(s) 354, 1626
HgaI GACGC 3 cut(s) 123, 827, 1166
Hin1II CATG 9 cut(s) 359, 458, 472, 707, 976, 1376, 1400, 1511, 1939
HincII GTYRAC 2 cut(s) 1154, 1758
HindII GTYRAC 2 cut(s) 1154, 1758
HindIII AAGCTT 2 cut(s) 255, 389
HpaI GTTAAC 1 cut(s) 1758
HphI GGTGA 6 cut(s) 428, 461, 952, 1436, 1559, 1837
Hpy166II GTNNAC 7 cut(s) 126, 304, 655, 715, 1154, 1564, 1758
Hpy188I TCNGA 9 cut(s) 64, 134, 502, 666, 1204, 1591, 1893, 1958, 2086
Hpy188III TCNNGA 5 cut(s) 791, 1391, 1728, 1916, 1994
Hpy8I GTNNAC 7 cut(s) 126, 304, 655, 715, 1154, 1564, 1758
HpyAV CCTTC 4 cut(s) 258, 773, 1049, 1717
HpyCH4III ACNGT 2 cut(s) 308, 427
HpyCH4IV ACGT 3 cut(s) 138, 169, 1002
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 56, 1302, 1882
HpySE526I ACGT 3 cut(s) 138, 169, 1002
Hsp92II CATG 9 cut(s) 359, 458, 472, 707, 976, 1376, 1400, 1511, 1939
Ksp22I TGATCA 6 cut(s) 451, 565, 661, 1353, 1393, 1947
KspAI GTTAAC 1 cut(s) 1758
LmnI GCTCC 4 cut(s) 885, 914, 1229, 2071
Lsp1109I GCAGC 3 cut(s) 712, 1356, 1860
LweI GCATC 7 cut(s) 139, 211, 1081, 1593, 1775, 1895, 2000
MaeI CTAG 6 cut(s) 294, 446, 729, 1679, 1820, 1929
MaeII ACGT 3 cut(s) 138, 169, 1002
MaeIII GTNAC 4 cut(s) 381, 724, 940, 1235
MbiI CCGCTC 1 cut(s) 321
MboII GAAGA 8 cut(s) 167, 410, 536, 643, 881, 1307, 1427, 1931
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 4 cut(s) 97, 229, 744, 1454
MhlI GDGCHC 1 cut(s) 144
MlsI TGGCCA 1 cut(s) 1626
MluNI TGGCCA 1 cut(s) 1626
MlyI GAGTC 4 cut(s) 27, 1159, 1371, 1559
MmeI TCCRAC 2 cut(s) 216, 964
Mox20I TGGCCA 1 cut(s) 1626
Mph1103I ATGCAT 2 cut(s) 1509, 1768
MscI TGGCCA 1 cut(s) 1626
MslI CAYNNNNRTG 2 cut(s) 447, 573
Msp20I TGGCCA 1 cut(s) 1626
MspCI CTTAAG 2 cut(s) 688, 1751
MspR9I CCNGG 1 cut(s) 11
MunI CAATTG 1 cut(s) 1271
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 4 cut(s) 11, 56, 1302, 1882
NcoI CCATGG 1 cut(s) 355
NlaIII CATG 9 cut(s) 359, 458, 472, 707, 976, 1376, 1400, 1511, 1939
NlaIV GGNNCC 4 cut(s) 916, 1125, 1576, 2067
NmuCI GTSAC 2 cut(s) 940, 1235
NsiI ATGCAT 2 cut(s) 1509, 1768
NspI RCATGY 1 cut(s) 707
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 8 cut(s) 1199, 1385, 1703, 1731, 1842, 1894, 1912, 2081
PflMI CCANNNNNTGG 2 cut(s) 2000, 2075
PkrI GCNGC 3 cut(s) 702, 1346, 1875
PleI GAGTC 4 cut(s) 27, 1158, 1371, 1559
PpsI GAGTC 4 cut(s) 27, 1158, 1371, 1559
Ppu21I YACGTR 1 cut(s) 170
PpuMI RGGWCCY 1 cut(s) 1522
PsiI TTATAA 1 cut(s) 1266
Psp5II RGGWCCY 1 cut(s) 1522
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 4 cut(s) 916, 1125, 1576, 2067
PspPI GGNCC 3 cut(s) 1522, 1575, 1668
PspPPI RGGWCCY 1 cut(s) 1522
PstI CTGCAG 3 cut(s) 105, 1573, 1878
PsuI RGATCY 4 cut(s) 97, 229, 744, 1454
RsaI GTAC 5 cut(s) 303, 656, 766, 1530, 2092
RsaNI GTAC 5 cut(s) 302, 655, 765, 1529, 2091
RseI CAYNNNNRTG 2 cut(s) 447, 573
SatI GCNGC 3 cut(s) 701, 1345, 1874
Sau96I GGNCC 3 cut(s) 1522, 1575, 1668
SchI GAGTC 4 cut(s) 27, 1159, 1371, 1559
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 7 cut(s) 139, 211, 1081, 1593, 1775, 1895, 2000
SfcI CTRYAG 4 cut(s) 101, 606, 1569, 1874
SinI GGWCC 3 cut(s) 1522, 1575, 1668
SmiMI CAYNNNNRTG 2 cut(s) 447, 573
SmlI CTYRAG 2 cut(s) 688, 1751
SmoI CTYRAG 2 cut(s) 688, 1751
SsiI CCGC 8 cut(s) 224, 319, 340, 980, 1116, 1666, 1830, 1848
SspI AATATT 1 cut(s) 1696
SspMI CTAG 6 cut(s) 294, 446, 729, 1679, 1820, 1929
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 4 cut(s) 109, 355, 748, 918
TaaI ACNGT 2 cut(s) 308, 427
TaiI ACGT 3 cut(s) 141, 172, 1005
TaqI TCGA 1 cut(s) 2026
TatI WGTACW 2 cut(s) 654, 1528
TfiI GAWTC 8 cut(s) 1199, 1385, 1703, 1731, 1842, 1894, 1912, 2081
TscAI CASTG 5 cut(s) 289, 430, 657, 717, 781
TseFI GTSAC 2 cut(s) 940, 1235
TseI GCWGC 3 cut(s) 700, 1344, 1873
Tsp45I GTSAC 2 cut(s) 940, 1235
TspDTI ATGAA 6 cut(s) 419, 485, 882, 1101, 1716, 1851
TspGWI ACGGA 2 cut(s) 9, 964
TspRI CASTG 5 cut(s) 289, 430, 657, 717, 781
Van91I CCANNNNNTGG 2 cut(s) 2000, 2075
Vha464I CTTAAG 2 cut(s) 688, 1751
VpaK11BI GGWCC 3 cut(s) 1522, 1575, 1668
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 4 cut(s) 406, 836, 994, 1334
XceI RCATGY 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 1563
XspI CTAG 6 cut(s) 294, 446, 729, 1679, 1820, 1929
Zsp2I ATGCAT 2 cut(s) 1509, 1768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.