Rh3DG275300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001156
Physical Location & Seq
Forward (+)
27903965 .. 27906550
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG275300.1

Sequence Viewer

Length: 1320 bp
ATGGCTGAACTTGTTCTTGATTTGGCAAGCAAGCTAACAGAGAGGTTGGGCTCCCTTGCTTACAATGAGATTTGCTTGGCATGGGGAGTCAAAACTGATCTGCAAAAGCTTGAGCGCACCCTGTCCACCATCAAAAGTGCGCTCTTTGATGCTGAAGATAAGCAAGTGGGTAACGAGGGGTTAAGAAGTTGGCTGCAACAACTTAAACATGTGTTCCGTGACGCAGAAGATGTATTGGATGAGTTTGAGTGCGAAGCTTTGCAGAGGCAAGTTGTAAAAAGCATTGCAAGAAAGTCAATTGGGCAAGATGAGTGGCGTGTTGTGGATTCTAGCACTAAAGACATCGTTGAAAATATTAGACACTTGTCATTTTTGGGATTTGACAACAAACTAATGGTTTCGATGTTGCAAAAGTTAAAAAAAGTGCGGAGTATAACTGCAATGGAAGAGGCGCCTATCGATGAATTATTCCTTGATACTTGCTTTTCAAAATTAAAATATTTGAGGGTGCTTCGCTTGGGTGATTTATCATTGAAGGAGCTGCCAAGTTCCATTGGTGCGTTAAAGCATTTAAGATATCTGCATTTAAAGGATGAGTTGCAGAGCTTGACAAAAATCCCCAATGCCATTTGCAAGTTGCAGAGCTTGCAAACTCTATTTTTTTTTGAGTGTGAAAATATTGAAGAGTTGCCAAGAGATATGAGCAAGTTGATCAGCCTCAGATTGCTTATGATGACGACGAAAGAAGCATGTTTTCAAGACAATGGAGTGGGATGTTTGAAATCACTTCGGTTTCTCGCGATTGTAAATTGTGGTAATTTAACCTCTTTGCCCCGCGAGATGAGTTACCTTGGGGCATTGCGGACATTGATATTAGCTGATTGTGAGCAACTCGATCTGGTGAGCCAACATTATAAAGCAATTCCATTGAGGCTCGAAAAGTTGGCTATTGATGGACTACCACGGATCACGGAGTTGCCTGACTGGTTTCGAGGAGCTGCCAACACCCTACAGCACCTGTACATTAAATATTGTTCCAATTTGGGTTCATTACCAGGGTGGCTCACAAATCTAACATCACTTAAAAAGCTTGTCCTCATTGATTGCCCCAAATTGTTGTCACTTCCAGAAGGGATGGAGCGTCTCACTGCCTTGAGAGACTTGAAGATAGAAGATTGTCCCGAGTTGGAGAGAAGATGCAAGCGTGACACTGGTGAAGATTGGCCAAAGATTGCTCGTATTCCAAACCTTTCTATATCAGACTGTGAAGAGTCAGATTCTGAAGAGTCAGATTCTGAAGTGTCAGACTCTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

439

Amino Acids

49.98

Weight (kDa)

5.34

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 94 1.7e-24 Rx N-terminal domain
LRR_14 PF23598 141 - 292 6e-19 Leucine-rich repeat region
LRR_14 PF23598 312 - 428 4.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 451
AccII CGCG 2 cut(s) 800, 837
AciI CCGC 3 cut(s) 427, 835, 862
AclWI GGATC 1 cut(s) 974
AcoI YGGCCR 1 cut(s) 1223
AcuI CTGAAG 3 cut(s) 174, 1302, 1317
AcyI GRCGYC 1 cut(s) 452
AfaI GTAC 1 cut(s) 1022
AflIII ACRYGT 1 cut(s) 208
AgsI TTSAA 7 cut(s) 350, 489, 535, 683, 758, 781, 1165
AjnI CCWGG 1 cut(s) 1054
AluBI AGCT 9 cut(s) 34, 109, 257, 541, 606, 645, 878, 998, 1090
AluI AGCT 9 cut(s) 34, 109, 257, 541, 606, 645, 878, 998, 1090
Alw26I GTCTC 2 cut(s) 1148, 1152
AlwI GGATC 1 cut(s) 974
AlwNI CAGNNNCTG 4 cut(s) 1018, 1280, 1295, 1310
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 1 cut(s) 1223
ApeKI GCWGC 3 cut(s) 193, 541, 998
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 3 cut(s) 117, 142, 454
AsuHPI GGTGA 3 cut(s) 533, 913, 1226
AvaI CYCGRG 1 cut(s) 1181
BalI TGGCCA 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 180, 528, 985
BccI CCATC 3 cut(s) 137, 947, 1129
BciT130I CCWGG 1 cut(s) 1056
BclI TGATCA 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 1148, 1152
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 1010
BfoI RGCGCY 1 cut(s) 455
BisI GCNGC 3 cut(s) 194, 542, 999
BlsI GCNGC 3 cut(s) 195, 543, 1000
Bme1390I CCNGG 1 cut(s) 1056
BmeT110I CYCGRG 1 cut(s) 1181
BmiI GGNNCC 2 cut(s) 52, 453
BmrFI CCNGG 1 cut(s) 1056
BmsI GCATC 2 cut(s) 139, 1187
BoxI GACNNNNGTC 1 cut(s) 364
BpuEI CTTGAG 2 cut(s) 131, 1174
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 452
BsaJI CCNNGG 3 cut(s) 850, 962, 1055
Bse1I ACTGG 2 cut(s) 989, 1216
Bse3DI GCAATG 3 cut(s) 282, 447, 857
BseBI CCWGG 1 cut(s) 1056
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 850, 962, 1055
BseGI GGATG 4 cut(s) 244, 598, 779, 1140
BseMI GCAATG 3 cut(s) 282, 447, 857
BseMII CTCAG 1 cut(s) 733
BseNI ACTGG 2 cut(s) 989, 1216
BseRI GAGGAG 1 cut(s) 1008
BseXI GCAGC 3 cut(s) 180, 528, 985
Bsh1236I CGCG 2 cut(s) 800, 837
BshFI GGCC 1 cut(s) 1225
BshNI GGYRCC 1 cut(s) 451
BshVI ATCGAT 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 1181
BslFI GGGAC 1 cut(s) 1164
BsmAI GTCTC 2 cut(s) 1148, 1152
BsmBI CGTCTC 1 cut(s) 1148
BsmFI GGGAC 1 cut(s) 1164
BsnI GGCC 1 cut(s) 1225
BsoBI CYCGRG 1 cut(s) 1181
Bsp1286I GDGCHC 1 cut(s) 53
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 4 cut(s) 97, 711, 895, 966
Bsp68I TCGCGA 1 cut(s) 800
BspACI CCGC 3 cut(s) 427, 835, 862
BspANI GGCC 1 cut(s) 1225
BspCNI CTCAG 1 cut(s) 732
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 2 cut(s) 800, 837
BspLI GGNNCC 2 cut(s) 52, 453
BspPI GGATC 1 cut(s) 974
BspT107I GGYRCC 1 cut(s) 451
BsrDI GCAATG 3 cut(s) 282, 447, 857
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 2 cut(s) 989, 1216
BssECI CCNNGG 3 cut(s) 850, 962, 1055
BssMI GATC 4 cut(s) 97, 711, 895, 966
BssNI GRCGYC 1 cut(s) 452
BssT1I CCWWGG 1 cut(s) 850
Bst2UI CCWGG 1 cut(s) 1056
Bst4CI ACNGT 1 cut(s) 1265
Bst6I CTCTTC 5 cut(s) 441, 678, 1263, 1278, 1308
BstACI GRCGYC 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 646
BstAUI TGTACA 1 cut(s) 1020
BstC8I GCNNGC 4 cut(s) 28, 32, 647, 1202
BstDEI CTNAG 1 cut(s) 719
BstDSI CCRYGG 1 cut(s) 962
BstF5I GGATG 4 cut(s) 244, 598, 779, 1140
BstFNI CGCG 2 cut(s) 800, 837
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 3 cut(s) 117, 142, 454
BstKTI GATC 4 cut(s) 100, 714, 898, 969
BstMAI GTCTC 2 cut(s) 1148, 1152
BstMBI GATC 4 cut(s) 97, 711, 895, 966
BstMWI GCNNNNNNNGC 1 cut(s) 646
BstNI CCWGG 1 cut(s) 1056
BstNSI RCATGY 2 cut(s) 212, 753
BstPAI GACNNNNGTC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 1010
BstUI CGCG 2 cut(s) 800, 837
BstV1I GCAGC 3 cut(s) 180, 528, 985
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 1 cut(s) 1225
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 962
BtsCI GGATG 4 cut(s) 244, 598, 779, 1140
BtsI GCAGTG 1 cut(s) 1146
BtsIMutI CAGTG 2 cut(s) 1146, 1209
BtuMI TCGCGA 1 cut(s) 800
Cac8I GCNNGC 4 cut(s) 28, 32, 647, 1202
CaiI CAGNNNCTG 4 cut(s) 1018, 1280, 1295, 1310
CfoI GCGC 3 cut(s) 117, 142, 454
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 2 cut(s) 230, 1130
Csp6I GTAC 1 cut(s) 1021
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviAII CATG 3 cut(s) 81, 209, 750
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 1 cut(s) 719
DinI GGCGCC 1 cut(s) 453
DpnI GATC 4 cut(s) 99, 713, 897, 968
DpnII GATC 4 cut(s) 97, 711, 895, 966
DraI TTTAAA 1 cut(s) 588
EaeI YGGCCR 1 cut(s) 1223
Eam1104I CTCTTC 5 cut(s) 441, 678, 1263, 1278, 1308
EarI CTCTTC 5 cut(s) 441, 678, 1263, 1278, 1308
Eco130I CCWWGG 1 cut(s) 850
Eco24I GRGCYC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 578
Eco57I CTGAAG 3 cut(s) 174, 1302, 1317
Eco88I CYCGRG 1 cut(s) 1181
EcoRII CCWGG 1 cut(s) 1054
EcoRV GATATC 1 cut(s) 578
EcoT14I CCWWGG 1 cut(s) 850
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 453
EheI GGCGCC 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 850
Esp3I CGTCTC 1 cut(s) 1148
FaeI CATG 3 cut(s) 84, 212, 753
FaiI YATR 8 cut(s) 82, 210, 434, 701, 731, 751, 915, 1256
FaqI GGGAC 1 cut(s) 1164
FatI CATG 3 cut(s) 80, 208, 749
FauI CCCGC 1 cut(s) 842
FbaI TGATCA 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 194, 542, 999
FokI GGATG 4 cut(s) 251, 605, 786, 1147
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 194, 542, 999
FspBI CTAG 1 cut(s) 330
GlaI GCGC 3 cut(s) 116, 141, 453
GluI GCNGC 3 cut(s) 194, 542, 999
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 1 cut(s) 1225
HgaI GACGC 2 cut(s) 230, 1130
HhaI GCGC 3 cut(s) 117, 142, 454
Hin1I GRCGYC 1 cut(s) 452
Hin1II CATG 3 cut(s) 84, 212, 753
Hin6I GCGC 3 cut(s) 115, 140, 452
HinP1I GCGC 3 cut(s) 115, 140, 452
HindIII AAGCTT 3 cut(s) 107, 255, 1088
HinfI GANTC 7 cut(s) 87, 326, 1271, 1277, 1286, 1292, 1307
HphI GGTGA 3 cut(s) 533, 913, 1226
Hpy166II GTNNAC 1 cut(s) 126
Hpy188I TCNGA 8 cut(s) 722, 1261, 1276, 1282, 1291, 1297, 1306, 1312
Hpy188III TCNNGA 5 cut(s) 17, 758, 799, 1127, 1181
Hpy8I GTNNAC 1 cut(s) 126
Hpy99I CGWCG 1 cut(s) 742
HpyAV CCTTC 2 cut(s) 529, 1124
HpyCH4III ACNGT 1 cut(s) 1265
HpyF10VI GCNNNNNNNGC 1 cut(s) 646
HpyF3I CTNAG 1 cut(s) 719
Hsp92I GRCGYC 1 cut(s) 452
Hsp92II CATG 3 cut(s) 84, 212, 753
HspAI GCGC 3 cut(s) 115, 140, 452
KasI GGCGCC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 711
Kzo9I GATC 4 cut(s) 97, 711, 895, 966
LmnI GCTCC 4 cut(s) 56, 538, 995, 1138
LpnPI CCDG 9 cut(s) 134, 884, 970, 993, 1031, 1041, 1068, 1140, 1197
Lsp1109I GCAGC 3 cut(s) 180, 528, 985
LweI GCATC 2 cut(s) 139, 1187
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 5 cut(s) 170, 218, 845, 1119, 1205
MalI GATC 4 cut(s) 99, 713, 897, 968
MboI GATC 4 cut(s) 97, 711, 895, 966
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 1 cut(s) 1225
MluCI AATT 8 cut(s) 297, 464, 491, 808, 817, 921, 1039, 1112
MluNI TGGCCA 1 cut(s) 1225
Mly113I GGCGCC 1 cut(s) 452
MlyI GAGTC 4 cut(s) 96, 1280, 1295, 1301
MmeI TCCRAC 1 cut(s) 1167
Mox20I TGGCCA 1 cut(s) 1225
MroXI GAANNNNTTC 1 cut(s) 12
MscI TGGCCA 1 cut(s) 1225
Msp20I TGGCCA 1 cut(s) 1225
MspR9I CCNGG 1 cut(s) 1056
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 1056
MvnI CGCG 2 cut(s) 800, 837
MwoI GCNNNNNNNGC 1 cut(s) 646
NarI GGCGCC 1 cut(s) 452
NdeII GATC 4 cut(s) 97, 711, 895, 966
NlaIII CATG 3 cut(s) 84, 212, 753
NlaIV GGNNCC 2 cut(s) 52, 453
NmuCI GTSAC 3 cut(s) 218, 1119, 1205
NruI TCGCGA 1 cut(s) 800
NspI RCATGY 2 cut(s) 212, 753
PciI ACATGT 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 326, 1277, 1292
PkrI GCNGC 3 cut(s) 195, 543, 1000
PleI GAGTC 4 cut(s) 95, 1279, 1294, 1301
PluTI GGCGCC 1 cut(s) 455
PpsI GAGTC 4 cut(s) 95, 1279, 1294, 1301
PscI ACATGT 1 cut(s) 208
PshAI GACNNNNGTC 1 cut(s) 364
PsiI TTATAA 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 1054
PspGI CCWGG 1 cut(s) 1054
PspN4I GGNNCC 2 cut(s) 52, 453
PstNI CAGNNNCTG 4 cut(s) 1018, 1280, 1295, 1310
RruI TCGCGA 1 cut(s) 800
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
SatI GCNGC 3 cut(s) 194, 542, 999
Sau3AI GATC 4 cut(s) 97, 711, 895, 966
SchI GAGTC 4 cut(s) 96, 1280, 1295, 1301
ScrFI CCNGG 1 cut(s) 1056
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 139, 1187
SfcI CTRYAG 1 cut(s) 1010
SfoI GGCGCC 1 cut(s) 453
SmlI CTYRAG 2 cut(s) 110, 1153
SmoI CTYRAG 2 cut(s) 110, 1153
Sse9I AATT 8 cut(s) 297, 464, 491, 808, 817, 921, 1039, 1112
SsiI CCGC 3 cut(s) 427, 835, 862
SspDI GGCGCC 1 cut(s) 451
SspI AATATT 4 cut(s) 355, 500, 679, 1031
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 1 cut(s) 1265
TaqI TCGA 5 cut(s) 401, 459, 894, 936, 991
TasI AATT 8 cut(s) 297, 464, 491, 808, 817, 921, 1039, 1112
TatI WGTACW 1 cut(s) 1020
TfiI GAWTC 3 cut(s) 326, 1277, 1292
TscAI CASTG 2 cut(s) 1153, 1216
TseFI GTSAC 3 cut(s) 218, 1119, 1205
TseI GCWGC 3 cut(s) 193, 541, 998
Tsp45I GTSAC 3 cut(s) 218, 1119, 1205
TspDTI ATGAA 2 cut(s) 477, 1038
TspGWI ACGGA 3 cut(s) 206, 979, 986
TspRI CASTG 2 cut(s) 1153, 1216
XceI RCATGY 2 cut(s) 212, 753
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.