pycom14g05810

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001156
Physical Location & Seq
Reverse (-)
5672158 .. 5672753
596 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05810.1

Sequence Viewer

Length: 555 bp
ATGAATCCTTCCTCATTAACCAATGCATCTTGCAATTTGTTGCCGGGTTCCATTGGTTCTTTGGAGCATTTGAGAAGCCTGCACTTGGCTAGAAATGAAAGAATCACCGCACTACCCAATTCAATTTGTAAGCTGCAGAGCTTGCAAACTCTGTTTATCCGATGCGTGAATCTTGAAGAGTTTCCTAGAGACATGAGAAAGTTGATCAGCCTCAGACGCCTTGTAATAACCACAAAACAATCAAGTTTTCCAGAGAATGGAGTGGGATGCTTGACATCACTTCGATATCTTTTTCTTGGGGAGTGTCATAATCTAACGTGTCTGCCGCGTGATACAAGTTATCTTGCGTCATTACACACTCTGATGATAATCAGCTGTAAACAACTTGATTTGGTGATGGAAAACTATCAGGTAATTCCACTAAGGCTCCAAAAATTGGGGATTGTAGAAGTACCACGAATGGTGGCATTGCCTGAATGGTTTCAAGGATGCACAAACACACTACGAGTCTTGATTATTGGGGATTGTGAGAATTTGGAGGCATTACCTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

185

Amino Acids

20.69

Weight (kDa)

8.41

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 16 - 123 1.2e-15 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 257, 436
AccII CGCG 1 cut(s) 328
AciI CCGC 2 cut(s) 108, 326
AcsI RAATTY 1 cut(s) 532
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 3 cut(s) 85, 257, 436
AflIII ACRYGT 1 cut(s) 317
AgsI TTSAA 3 cut(s) 123, 176, 485
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 3 cut(s) 133, 141, 375
AluI AGCT 3 cut(s) 133, 141, 375
Alw26I GTCTC 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 532
Asp700I GAANNNNTTC 2 cut(s) 180, 480
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 97, 406
BbvI GCAGC 1 cut(s) 120
BccI CCATC 1 cut(s) 391
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 2 cut(s) 90, 186
BfmI CTRYAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 134, 326
BlsI GCNGC 2 cut(s) 135, 327
Bme1390I CCNGG 2 cut(s) 45, 549
BmiI GGNNCC 2 cut(s) 49, 428
BmrFI CCNGG 2 cut(s) 45, 549
BmsI GCATC 4 cut(s) 35, 152, 257, 479
BpuMI CCSGG 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 257, 436
Bse3DI GCAATG 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 368
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 2 cut(s) 272, 494
BseJI GATNNNNATC 1 cut(s) 368
BseLI CCNNNNNNNGG 3 cut(s) 85, 257, 436
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 328
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 3 cut(s) 85, 257, 436
BsmAI GTCTC 1 cut(s) 183
Bsp143I GATC 1 cut(s) 204
BspACI CCGC 2 cut(s) 108, 326
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 49, 428
BspMAI CTGCAG 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 1 cut(s) 204
BssNI GRCGYC 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 549
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 80, 143
BstDEI CTNAG 2 cut(s) 212, 422
BstF5I GGATG 2 cut(s) 272, 494
BstFNI CGCG 1 cut(s) 328
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 142, 216
BstNI CCWGG 1 cut(s) 549
BstSCI CCNGG 2 cut(s) 43, 547
BstSFI CTRYAG 1 cut(s) 134
BstUI CGCG 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 120
BtsCI GGATG 2 cut(s) 272, 494
Cac8I GCNNGC 2 cut(s) 80, 143
CseI GACGC 2 cut(s) 225, 336
Csp6I GTAC 1 cut(s) 452
CviAII CATG 1 cut(s) 193
CviJI RGCY 7 cut(s) 78, 89, 133, 141, 210, 375, 427
CviKI_1 RGCY 7 cut(s) 78, 89, 133, 141, 210, 375, 427
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 2 cut(s) 212, 422
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 547
EcoRV GATATC 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 28
FaeI CATG 1 cut(s) 196
FaiI YATR 2 cut(s) 194, 309
FatI CATG 1 cut(s) 192
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 134, 326
FokI GGATG 2 cut(s) 279, 501
Fsp4HI GCNGC 2 cut(s) 134, 326
FspBI CTAG 2 cut(s) 90, 186
GluI GCNGC 2 cut(s) 134, 326
HapII CCGG 1 cut(s) 44
HgaI GACGC 2 cut(s) 225, 336
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 1 cut(s) 196
HinfI GANTC 4 cut(s) 4, 102, 169, 507
HpaII CCGG 1 cut(s) 44
HphI GGTGA 2 cut(s) 97, 406
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 3 cut(s) 161, 215, 363
Hpy188III TCNNGA 3 cut(s) 173, 251, 511
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 1 cut(s) 18
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 6 cut(s) 26, 33, 82, 136, 145, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 216
HpyF3I CTNAG 2 cut(s) 212, 422
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 64, 432
LpnPI CCDG 6 cut(s) 57, 92, 264, 395, 486, 534
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 4 cut(s) 35, 152, 257, 479
MaeI CTAG 2 cut(s) 90, 186
MaeII ACGT 1 cut(s) 317
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 1 cut(s) 188
MluCI AATT 6 cut(s) 34, 118, 123, 414, 434, 532
MlyI GAGTC 1 cut(s) 516
MnlI CCTC 3 cut(s) 22, 221, 532
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 2 cut(s) 180, 480
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 45, 549
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 2 cut(s) 142, 216
NciI CCSGG 1 cut(s) 45
NdeII GATC 1 cut(s) 204
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 2 cut(s) 49, 428
NsiI ATGCAT 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 180, 480
PfeI GAWTC 3 cut(s) 4, 102, 169
PflMI CCANNNNNTGG 2 cut(s) 257, 436
PkrI GCNGC 2 cut(s) 135, 327
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 2 cut(s) 49, 428
PstI CTGCAG 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 375
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 2 cut(s) 134, 326
Sau3AI GATC 1 cut(s) 204
SchI GAGTC 1 cut(s) 516
ScrFI CCNGG 2 cut(s) 45, 549
SetI ASST 6 cut(s) 135, 143, 320, 377, 414, 550
SfaNI GCATC 4 cut(s) 35, 152, 257, 479
SfcI CTRYAG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 362
Sse9I AATT 6 cut(s) 34, 118, 123, 414, 434, 532
SsiI CCGC 2 cut(s) 108, 326
SspMI CTAG 2 cut(s) 90, 186
StyD4I CCNGG 2 cut(s) 43, 547
TaiI ACGT 1 cut(s) 320
TaqI TCGA 1 cut(s) 283
TasI AATT 6 cut(s) 34, 118, 123, 414, 434, 532
TauI GCSGC 1 cut(s) 328
TfiI GAWTC 3 cut(s) 4, 102, 169
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 17, 111
Van91I CCANNNNNTGG 2 cut(s) 257, 436
XapI RAATTY 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XmnI GAANNNNTTC 2 cut(s) 180, 480
XspI CTAG 2 cut(s) 90, 186
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.