AT1G61820

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0017061
Physical Location & Seq
Forward (+)
22835078 .. 22838635
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61820.1

Sequence Viewer

Length: 1551 bp
ATGAAAACTTTTGCCAACTTTGCAATTCTGTTTTTATTACAAAGCTTATTGTTTCCTCTTTACAGTTCATGCCTTCATCAAACTTCAGATGATTCATCTCCATTTCCTTCTGATTTCCTCTTTGGCACAGCTTCTTCTGCTTTCCAGTATGAAGGTGCCTTCTTAACCGATGGAAAAGGTCTGAACAATTGGGATGTCTTTGCCCATGAAAACCCTGGGAAAATAGTTGATGGAAGCAATGGAGACATAGCTACGGACCAATATCATCGATATATGGAAGATATCCAATCAATGAATTTTCTTGGAGTCAATAGTTACAGATTATCTATTTCCTGGTCTAGAGTCTTACCTAATGGAAGATTTGGAGTTATTAATTATAAGGGAATAAAGTATTACAACAATTTGATCGACGCTCTTATTAAAAAAGGGATTACACCATTTGTGACGTTGAACCACTTCGACTATCCTCAAGAACTCGAGAACCGGTTCAAGAGTTGGTTAAGCTCCGAGATGCAGAAAGATTTCGGGTACTTAGCTGATATATGTTTCAAACATTTTGGAGACCGAGTTAAACACTGGATCACGATTAACGAACCAAACCAACATATAAGCTTAGCATATCGAAGTGGTTTGTTTCCACCAGCGCGGTGCTCCATGCCATACGGAAATTGTACTCACGGGAACTCAGAAACTGAGCCTTTTATAGCCGCTCATAATATGATCCTCGCACATGCAAAGGCGATTCAAATATACCGAACCAAATATCAAAGAGAACAAAAGGGAATCATTGGCATTGTGGTACAAACATCATGGTTTGAACCCATAAGCGATTCGATTGCTGATAAAAATGCTGCCGAGAGAGCTCAATCATTTTATTCAAATTGGATTCTAGATCCCGTTGTGTATGGGAAATATCCGGAAGAAATGGTGAATCTACTTGGATCAGCTTTGCCAAAATTTTCGAGCAATGAAATGAACAGCCTAATGAGCTATAAATCAGACTTTTTGGGTATTAATCACTATACAAGTTACTTCATACAAGATTGTTTGATCACTGCTTGTAATTCTGGAGACGGAGCTTCTAAAAGCGAAGGATTAGCCCTCAAATTGGACCGGAAAGGAAATGTTTCCATCGGAGAACTGACCGACGTAAATTGGCAGCATATTGATCCTAACGGATTCAGAAAGATGTTGAATTACCTAAAAAATAGGTACCACAACATACCAATGTACATCACCGAAAATGGTTTCGGACAGTTGCAAAAACCAGAGACAACAGTTGAAGAACTTCTGCATGATACAAAAAGGATACAATACTTGAGTGGATATTTGGATGCTTTGAAAGCAGCAATGAGGGATGGAGCAAATGTGAAGGGATATTTCGCATGGTCACTATTAGATAATTTTGAATGGTTGTACGGATACAAGGTTCGATTTGGGCTATTCCACGTTGATTTTACAACTTTGAAAAGGACACCAAAGCAATCAGCTACATGGTACAAGAACTTCATTGAACAGAACGTGAATATAGAAGATCAAATAGATAAATAA

Protein Analysis

516

Amino Acids

59.11

Weight (kDa)

6.44

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 33 - 507 9.3e-152 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 2 cut(s) 155, 1212
AccBSI CCGCTC 1 cut(s) 710
AccII CGCG 1 cut(s) 646
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 2 cut(s) 646, 708
AclWI GGATC 5 cut(s) 587, 715, 887, 949, 1163
AcsI RAATTY 2 cut(s) 295, 956
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 7 cut(s) 530, 673, 801, 1214, 1232, 1418, 1499
AfiI CCNNNNNNNGG 1 cut(s) 1108
AgeI ACCGGT 1 cut(s) 483
AjnI CCWGG 2 cut(s) 214, 332
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
Alw21I GWGCWC 2 cut(s) 653, 865
Alw26I GTCTC 4 cut(s) 237, 555, 1065, 1265
AlwI GGATC 5 cut(s) 587, 715, 887, 949, 1163
AlwNI CAGNNNCTG 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 916
ApeKI GCWGC 3 cut(s) 851, 1159, 1346
ApoI RAATTY 2 cut(s) 295, 956
AseI ATTAAT 2 cut(s) 372, 1014
AsiGI ACCGGT 1 cut(s) 483
Asp700I GAANNNNTTC 5 cut(s) 455, 485, 521, 1126, 1287
Asp718I GGTACC 1 cut(s) 1212
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 2 cut(s) 256, 1111
AsuHPI GGTGA 2 cut(s) 940, 1228
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 2 cut(s) 256, 1111
BanI GGYRCC 2 cut(s) 155, 1212
BanII GRGCYC 1 cut(s) 865
BarI GAAGNNNNNNTAC 2 cut(s) 1490, 1522
Bbv12I GWGCWC 2 cut(s) 653, 865
BbvI GCAGC 3 cut(s) 838, 1171, 1358
BccI CCATC 4 cut(s) 164, 224, 1139, 1352
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 2 cut(s) 216, 334
BciVI GTATCC 2 cut(s) 1302, 1415
BclI TGATCA 1 cut(s) 1050
BcoDI GTCTC 4 cut(s) 237, 555, 1065, 1265
BfaI CTAG 2 cut(s) 339, 890
BfuI GTATCC 2 cut(s) 1302, 1415
BisI GCNGC 4 cut(s) 708, 852, 1160, 1347
BlpI GCTNAGC 1 cut(s) 613
BlsI GCNGC 4 cut(s) 709, 853, 1161, 1348
Bme1390I CCNGG 2 cut(s) 216, 334
Bme18I GGWCC 2 cut(s) 256, 1111
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 2 cut(s) 256, 1111
BmiI GGNNCC 2 cut(s) 157, 1214
BmrFI CCNGG 2 cut(s) 216, 334
BmsI GCATC 2 cut(s) 501, 1324
BpmI CTGGAG 1 cut(s) 1089
Bpu1102I GCTNAGC 1 cut(s) 613
BpuEI CTTGAG 2 cut(s) 453, 1339
Bsa29I ATCGAT 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 214, 215
BsaWI WCCGGW 3 cut(s) 483, 916, 1113
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 1353, 1383
Bsc4I CCNNNNNNNGG 1 cut(s) 1108
Bse118I RCCGGY 1 cut(s) 483
Bse1I ACTGG 2 cut(s) 145, 581
Bse3DI GCAATG 3 cut(s) 244, 973, 1356
BseAI TCCGGA 1 cut(s) 916
BseBI CCWGG 2 cut(s) 216, 334
BseCI ATCGAT 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 214, 215
BseGI GGATG 3 cut(s) 199, 1339, 1363
BseLI CCNNNNNNNGG 1 cut(s) 1108
BseMI GCAATG 3 cut(s) 244, 973, 1356
BseMII CTCAG 2 cut(s) 684, 699
BseNI ACTGG 2 cut(s) 145, 581
BseXI GCAGC 3 cut(s) 838, 1171, 1358
Bsh1236I CGCG 1 cut(s) 646
BshNI GGYRCC 2 cut(s) 155, 1212
BshTI ACCGGT 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 268
BsiHKAI GWGCWC 2 cut(s) 653, 865
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 3 cut(s) 484, 917, 1114
BslI CCNNNNNNNGG 1 cut(s) 1108
BsmAI GTCTC 4 cut(s) 237, 555, 1065, 1265
BsmBI CGTCTC 1 cut(s) 1065
Bso31I GGTCTC 1 cut(s) 555
BsoBI CYCGRG 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 653, 865
Bsp13I TCCGGA 1 cut(s) 916
Bsp1407I TGTACA 1 cut(s) 1230
Bsp143I GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
Bsp1720I GCTNAGC 1 cut(s) 613
BspACI CCGC 2 cut(s) 646, 708
BspCNI CTCAG 2 cut(s) 685, 698
BspDI ATCGAT 1 cut(s) 268
BspEI TCCGGA 1 cut(s) 916
BspFNI CGCG 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 157, 1214
BspPI GGATC 5 cut(s) 587, 715, 887, 949, 1163
BspT107I GGYRCC 2 cut(s) 155, 1212
BspTNI GGTCTC 1 cut(s) 555
BsrBI CCGCTC 1 cut(s) 710
BsrDI GCAATG 3 cut(s) 244, 973, 1356
BsrFI RCCGGY 1 cut(s) 483
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 2 cut(s) 145, 581
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 2 cut(s) 214, 215
BssMI GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
Bst2UI CCWGG 2 cut(s) 216, 334
Bst4CI ACNGT 3 cut(s) 65, 1257, 1279
BstAUI TGTACA 1 cut(s) 1230
BstDEI CTNAG 4 cut(s) 532, 613, 685, 693
BstF5I GGATG 3 cut(s) 199, 1339, 1363
BstFNI CGCG 1 cut(s) 646
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 8 cut(s) 408, 582, 723, 895, 944, 1053, 1171, 1537
BstMAI GTCTC 4 cut(s) 237, 555, 1065, 1265
BstMBI GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
BstMWI GCNNNNNNNGC 5 cut(s) 20, 137, 860, 987, 1343
BstNI CCWGG 2 cut(s) 216, 334
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 2 cut(s) 214, 332
BstUI CGCG 1 cut(s) 646
BstV1I GCAGC 3 cut(s) 838, 1171, 1358
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 268
BsuI GTATCC 2 cut(s) 1302, 1415
BsuTUI ATCGAT 1 cut(s) 268
BtsCI GGATG 3 cut(s) 199, 1339, 1363
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 2 cut(s) 574, 1053
CaiI CAGNNNCTG 1 cut(s) 692
CfoI GCGC 1 cut(s) 646
Cfr10I RCCGGY 1 cut(s) 483
Cfr13I GGNCC 2 cut(s) 256, 1111
ClaI ATCGAT 1 cut(s) 268
CseI GACGC 1 cut(s) 419
Csp6I GTAC 7 cut(s) 529, 672, 800, 1213, 1231, 1417, 1498
CspAI ACCGGT 1 cut(s) 483
CviAII CATG 8 cut(s) 69, 206, 655, 731, 810, 1295, 1386, 1494
CviQI GTAC 7 cut(s) 529, 672, 800, 1213, 1231, 1417, 1498
DdeI CTNAG 4 cut(s) 532, 613, 685, 693
DpnI GATC 8 cut(s) 407, 581, 722, 894, 943, 1052, 1170, 1536
DpnII GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
Ecl136II GAGCTC 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 865
Eco31I GGTCTC 1 cut(s) 555
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 256, 1111
Eco53kI GAGCTC 1 cut(s) 863
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 863
EcoRII CCWGG 2 cut(s) 214, 332
EcoRV GATATC 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 865
Esp3I CGTCTC 1 cut(s) 1065
FaeI CATG 8 cut(s) 72, 209, 658, 734, 813, 1298, 1389, 1497
FatI CATG 8 cut(s) 68, 205, 654, 730, 809, 1294, 1385, 1493
FbaI TGATCA 1 cut(s) 1050
Fnu4HI GCNGC 4 cut(s) 708, 852, 1160, 1347
FokI GGATG 3 cut(s) 206, 1346, 1370
FriOI GRGCYC 1 cut(s) 865
Fsp4HI GCNGC 4 cut(s) 708, 852, 1160, 1347
FspBI CTAG 2 cut(s) 339, 890
GlaI GCGC 1 cut(s) 645
GluI GCNGC 4 cut(s) 708, 852, 1160, 1347
GsuI CTGGAG 1 cut(s) 1089
HapII CCGG 3 cut(s) 484, 917, 1114
HgaI GACGC 1 cut(s) 419
HhaI GCGC 1 cut(s) 646
Hin1II CATG 8 cut(s) 72, 209, 658, 734, 813, 1298, 1389, 1497
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HindIII AAGCTT 2 cut(s) 43, 610
HinfI GANTC 9 cut(s) 92, 306, 342, 742, 783, 830, 886, 931, 1179
HpaII CCGG 3 cut(s) 484, 917, 1114
HphI GGTGA 2 cut(s) 940, 1228
Hpy188I TCNGA 9 cut(s) 88, 112, 183, 508, 688, 1000, 1136, 1184, 1253
Hpy188III TCNNGA 8 cut(s) 339, 470, 478, 490, 583, 890, 917, 1068
Hpy99I CGWCG 2 cut(s) 413, 1151
HpyAV CCTTC 6 cut(s) 83, 117, 146, 169, 1085, 1366
HpyCH4III ACNGT 3 cut(s) 65, 1257, 1279
HpyCH4IV ACGT 4 cut(s) 446, 1149, 1449, 1521
HpyCH4V TGCA 5 cut(s) 23, 514, 734, 1261, 1294
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 137, 860, 987, 1343
HpyF3I CTNAG 4 cut(s) 532, 613, 685, 693
HpySE526I ACGT 4 cut(s) 446, 1149, 1449, 1521
Hsp92II CATG 8 cut(s) 72, 209, 658, 734, 813, 1298, 1389, 1497
HspAI GCGC 1 cut(s) 644
Kpn2I TCCGGA 1 cut(s) 916
KpnI GGTACC 1 cut(s) 1216
Ksp22I TGATCA 1 cut(s) 1050
Kzo9I GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
LmnI GCTCC 4 cut(s) 509, 656, 1076, 1361
Lsp1109I GCAGC 3 cut(s) 838, 1171, 1358
LweI GCATC 2 cut(s) 501, 1324
MaeI CTAG 2 cut(s) 339, 890
MaeII ACGT 4 cut(s) 446, 1149, 1449, 1521
MaeIII GTNAC 4 cut(s) 314, 442, 1028, 1389
MalI GATC 8 cut(s) 407, 581, 722, 894, 943, 1052, 1170, 1536
MbiI CCGCTC 1 cut(s) 710
MboI GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
MboII GAAGA 6 cut(s) 126, 290, 369, 932, 1295, 1544
MfeI CAATTG 1 cut(s) 187
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 2 cut(s) 653, 865
MlyI GAGTC 2 cut(s) 315, 351
MnlI CCTC 6 cut(s) 66, 128, 477, 734, 1112, 1347
MroI TCCGGA 1 cut(s) 916
MroXI GAANNNNTTC 5 cut(s) 455, 485, 521, 1126, 1287
MseI TTAA 7 cut(s) 164, 372, 420, 500, 570, 588, 1014
MslI CAYNNNNRTG 1 cut(s) 1226
MspI CCGG 3 cut(s) 484, 917, 1114
MspR9I CCNGG 2 cut(s) 216, 334
MunI CAATTG 1 cut(s) 187
MvaI CCWGG 2 cut(s) 216, 334
MvnI CGCG 1 cut(s) 646
MwoI GCNNNNNNNGC 5 cut(s) 20, 137, 860, 987, 1343
NdeII GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
NlaIII CATG 8 cut(s) 72, 209, 658, 734, 813, 1298, 1389, 1497
NlaIV GGNNCC 2 cut(s) 157, 1214
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 2 cut(s) 442, 1389
NspI RCATGY 1 cut(s) 734
PaeR7I CTCGAG 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 215
PdmI GAANNNNTTC 5 cut(s) 455, 485, 521, 1126, 1287
PfeI GAWTC 7 cut(s) 92, 742, 783, 830, 886, 931, 1179
PinAI ACCGGT 1 cut(s) 483
PkrI GCNGC 4 cut(s) 709, 853, 1161, 1348
PleI GAGTC 2 cut(s) 314, 350
PpsI GAGTC 2 cut(s) 314, 350
PshBI ATTAAT 2 cut(s) 372, 1014
PsiI TTATAA 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 865
Psp6I CCWGG 2 cut(s) 214, 332
PspGI CCWGG 2 cut(s) 214, 332
PspN4I GGNNCC 2 cut(s) 157, 1214
PspPI GGNCC 2 cut(s) 256, 1111
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 1 cut(s) 892
RsaI GTAC 7 cut(s) 530, 673, 801, 1214, 1232, 1418, 1499
RsaNI GTAC 7 cut(s) 529, 672, 800, 1213, 1231, 1417, 1498
RseI CAYNNNNRTG 1 cut(s) 1226
SacI GAGCTC 1 cut(s) 865
SaqAI TTAA 7 cut(s) 164, 372, 420, 500, 570, 588, 1014
SatI GCNGC 4 cut(s) 708, 852, 1160, 1347
Sau3AI GATC 8 cut(s) 405, 579, 720, 892, 941, 1050, 1168, 1534
Sau96I GGNCC 2 cut(s) 256, 1111
SchI GAGTC 2 cut(s) 315, 351
ScrFI CCNGG 2 cut(s) 216, 334
SduI GDGCHC 2 cut(s) 653, 865
SfaNI GCATC 2 cut(s) 501, 1324
Sfr274I CTCGAG 1 cut(s) 476
SinI GGWCC 2 cut(s) 256, 1111
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 1226
SmlI CTYRAG 3 cut(s) 468, 476, 1318
SmoI CTYRAG 3 cut(s) 468, 476, 1318
SsiI CCGC 2 cut(s) 646, 708
SspMI CTAG 2 cut(s) 339, 890
SstI GAGCTC 1 cut(s) 865
StyD4I CCNGG 2 cut(s) 214, 332
TaaI ACNGT 3 cut(s) 65, 1257, 1279
TaiI ACGT 4 cut(s) 449, 1152, 1452, 1524
TaqI TCGA 8 cut(s) 268, 408, 459, 477, 622, 833, 962, 1432
TaqII GACCGA 2 cut(s) 579, 1160
TatI WGTACW 2 cut(s) 671, 1230
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 7 cut(s) 92, 742, 783, 830, 886, 931, 1179
Tru1I TTAA 7 cut(s) 164, 372, 420, 500, 570, 588, 1014
Tru9I TTAA 7 cut(s) 164, 372, 420, 500, 570, 588, 1014
TscAI CASTG 2 cut(s) 581, 1060
TseFI GTSAC 2 cut(s) 442, 1389
TseI GCWGC 3 cut(s) 851, 1159, 1346
Tsp45I GTSAC 2 cut(s) 442, 1389
TspGWI ACGGA 5 cut(s) 269, 678, 1089, 1191, 1434
TspRI CASTG 2 cut(s) 581, 1060
VpaK11BI GGWCC 2 cut(s) 256, 1111
VspI ATTAAT 2 cut(s) 372, 1014
XapI RAATTY 2 cut(s) 295, 956
XbaI TCTAGA 2 cut(s) 338, 889
XceI RCATGY 1 cut(s) 734
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 476
XmnI GAANNNNTTC 5 cut(s) 455, 485, 521, 1126, 1287
XspI CTAG 2 cut(s) 339, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.