MD11G1240900.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017061
Physical Location & Seq
Forward (+)
35037164 .. 35041131
3968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1240900.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGAAATTTACTGGAGGCTTGTTCTATTTCTTTTCGCTCTCGGTTATCTTATTGTCTACCGCTTTAGTTGCAGCTCAGAGTTTCAAACCAGATGAAGAAGAAATCCAGAAATCTCAGTTCCCAGATGGCTTCTTGTTTGGAGCAAGCACTTCATCTTATCAGATTGAAGGTGCTTATCATGAAGATGGAAAGAGCCTAAGCAACTGGGATGTTTATGCTCACATTCCAGGAAAAATAGAGGATAATGAGACTGGAGATGTTGCAGATGACCATTACCATCGCTACTTGGAGGACATTGAGTGGATACATTCTCTGGGGATGAATGCATATCGGTTCTCCATTTCATGGGCTAGAATTCTACCTGACGGCAAACTCGGCAAAGTCAATCAAAGAGGGATCATATTTTATAATAAACTTATTGATCATCTTGTGAGCAAAGGAATAGAGCCATTTGTGACACTTCACCACAATGACATGCCCTATATACTAGATGCAAGAGATAGGGGTTGGCTTAGTCCACTCTTACGGGAAGAATTTGCACATTTTGCCTCCATATGCTTCAAGAGCTTTGGGGACAGGGTGAAGCACTGGATTACCTTCAATGAAGCCAATATGCTGGTTGATTTTACTTACATACAAGGAGTATACCCACCTGCTCGTTGCACGCCCCCTTTTGGGAATTGTTCAAGTGGCAATTCTGATGTAGAGCCTCTCATTGCAATGCACAACATTTTAATAGCTCATGCCATGGCGGTTGACATATATCGGAGGCTTTTTCAGCCAAAACAACATGGGTTTGTCGGAATTATTGCACATTGTCACTGGTATGAACCTCTTCGAGACGATGAAAGGGACCTGCATGCTGTTGATAGAGTCTTAGCTTTCAATGCGGCCTGGGTGTTGGATCCTTTAGTGTATGGAGAATACCCCGCTGAAATGCGCCAATGCCCTCGCTTGGAAATGCCAAGGTTCTCCCCCAAAGAAAAAGAGCTGTTAAGAGGCAGCATAGATTTCATCGGACTCAACCACTATACAACACTATATGCCAAGGACTGTTTCCACTCCCCTTGCCCCTCAGGCGGAGACCATGCCATTAGAGGTTACGTCAACACAACTGGGTATCGGGATGGCACTGCGATTGGAGAACTGACTGGAGTTGCTAGGTTTTTTGTTGTTCCAAGAGGCATGGAGAAGATTATAGACTATCTTAAGCAAAGATACAATAACCTGCCCATGTTTGTTACTGAAAATGGATACTCCTCACCGACACCACAGAATGAGCTACAAGACTGGAATCGGATCAAGTTTCACAAAGGCTACCTAGCTTTTTTAGCCAAAGCAATAAGGAATGGTGCTGATGTACGTGGCTATTTCATTTGGAGCTTGATGGATAATTTTGAATGGCTTAGTGGGTATGAAACAAAATTTGGTATAGTTTATGTTGATCGTAATAAAACGCTGGGGCGAACTCCAAAGCCTTCTGCAAGGTGGTTTAGTAGTTTCATCAGAAACAATAGTCACAATGAGGAAGAAATTAGTGCAGCTTCTTCAGGTCACTCCAACAAAAATACACTATTTAGCCAATTGCAACCGAAGGTTGCTGAAATGTAG

Protein Analysis

537

Amino Acids

61.37

Weight (kDa)

6.27

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 37 - 506 8.2e-147 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AarI CACCTGC 1 cut(s) 661
Acc36I ACCTGC 3 cut(s) 661, 864, 1236
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 2 cut(s) 56, 645
AciI CCGC 5 cut(s) 60, 752, 890, 930, 1080
AclWI GGATC 4 cut(s) 404, 899, 912, 1307
AcsI RAATTY 4 cut(s) 5, 354, 533, 1424
AcuI CTGAAG 1 cut(s) 1533
AfaI GTAC 1 cut(s) 1362
AfiI CCNNNNNNNGG 5 cut(s) 345, 674, 675, 955, 1079
AflII CTTAAG 1 cut(s) 1208
AgsI TTSAA 7 cut(s) 85, 167, 562, 601, 687, 886, 1400
AjnI CCWGG 2 cut(s) 226, 893
AjuI GAANNNNNNNTTGG 4 cut(s) 1040, 1072, 1410, 1442
AluBI AGCT 9 cut(s) 74, 567, 740, 881, 991, 1282, 1325, 1383, 1544
AluI AGCT 9 cut(s) 74, 567, 740, 881, 991, 1282, 1325, 1383, 1544
Alw26I GTCTC 3 cut(s) 242, 834, 1077
AlwI GGATC 4 cut(s) 404, 899, 912, 1307
AoxI GGCC 1 cut(s) 891
ApeKI GCWGC 3 cut(s) 71, 1002, 1541
ApoI RAATTY 4 cut(s) 5, 354, 533, 1424
Asp700I GAANNNNTTC 1 cut(s) 834
AspLEI GCGC 1 cut(s) 942
AspS9I GGNCC 1 cut(s) 853
AsuHPI GGTGA 3 cut(s) 455, 592, 1254
AvaII GGWCC 1 cut(s) 853
AxyI CCTNAGG 1 cut(s) 1075
BamHI GGATCC 1 cut(s) 904
BbvI GCAGC 3 cut(s) 83, 1014, 1553
BccI CCATC 5 cut(s) 119, 179, 285, 1121, 1381
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 2 cut(s) 228, 895
BciVI GTATCC 2 cut(s) 297, 1247
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 3 cut(s) 242, 834, 1077
BfaI CTAG 4 cut(s) 351, 488, 1161, 1322
BfrI CTTAAG 1 cut(s) 1208
BfuAI ACCTGC 3 cut(s) 661, 864, 1236
BfuI GTATCC 2 cut(s) 297, 1247
BglI GCCNNNNNGGC 1 cut(s) 1077
BisI GCNGC 4 cut(s) 72, 891, 1003, 1542
BlsI GCNGC 4 cut(s) 73, 892, 1004, 1543
Bme1390I CCNGG 2 cut(s) 228, 895
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmiI GGNNCC 2 cut(s) 854, 906
BmrFI CCNGG 2 cut(s) 228, 895
BmrI ACTGGG 2 cut(s) 214, 1125
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 2 cut(s) 214, 1125
BpmI CTGGAG 3 cut(s) 33, 273, 1173
Bpu10I CCTNAGC 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 1364
BsaI GGTCTC 1 cut(s) 1077
BsaJI CCNNGG 4 cut(s) 747, 894, 965, 1047
Bsc4I CCNNNNNNNGG 5 cut(s) 345, 674, 675, 955, 1079
Bse1I ACTGG 8 cut(s) 16, 209, 256, 593, 827, 1120, 1156, 1295
Bse21I CCTNAGG 1 cut(s) 1075
Bse3DI GCAATG 2 cut(s) 714, 726
BseBI CCWGG 2 cut(s) 228, 895
BseDI CCNNGG 4 cut(s) 747, 894, 965, 1047
BseGI GGATG 3 cut(s) 214, 324, 1132
BseLI CCNNNNNNNGG 5 cut(s) 345, 674, 675, 955, 1079
BseMI GCAATG 2 cut(s) 714, 726
BseMII CTCAG 3 cut(s) 89, 128, 1089
BseNI ACTGG 8 cut(s) 16, 209, 256, 593, 827, 1120, 1156, 1295
BseRI GAGGAG 1 cut(s) 1249
BseXI GCAGC 3 cut(s) 83, 1014, 1553
BseYI CCCAGC 1 cut(s) 1459
BsgI GTGCAG 1 cut(s) 1560
BshFI GGCC 1 cut(s) 893
BslFI GGGAC 2 cut(s) 587, 866
BslI CCNNNNNNNGG 5 cut(s) 345, 674, 675, 955, 1079
BsmAI GTCTC 3 cut(s) 242, 834, 1077
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 2 cut(s) 587, 866
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 893
Bso31I GGTCTC 1 cut(s) 1077
Bsp143I GATC 5 cut(s) 396, 421, 904, 1299, 1444
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 5 cut(s) 60, 752, 890, 930, 1080
BspANI GGCC 1 cut(s) 893
BspCNI CTCAG 3 cut(s) 88, 127, 1088
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 854, 906
BspMI ACCTGC 3 cut(s) 661, 864, 1236
BspPI GGATC 4 cut(s) 404, 899, 912, 1307
BspTI CTTAAG 1 cut(s) 1208
BspTNI GGTCTC 1 cut(s) 1077
BsrDI GCAATG 2 cut(s) 714, 726
BsrI ACTGG 8 cut(s) 16, 209, 256, 593, 827, 1120, 1156, 1295
BssECI CCNNGG 4 cut(s) 747, 894, 965, 1047
BssMI GATC 5 cut(s) 396, 421, 904, 1299, 1444
BssNAI GTATAC 1 cut(s) 646
BssT1I CCWWGG 3 cut(s) 747, 965, 1047
Bst1107I GTATAC 1 cut(s) 646
Bst2UI CCWGG 2 cut(s) 228, 895
Bst4CI ACNGT 1 cut(s) 1055
Bst6I CTCTTC 1 cut(s) 840
BstAFI CTTAAG 1 cut(s) 1208
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 1364
BstC8I GCNNGC 3 cut(s) 145, 665, 861
BstDEI CTNAG 7 cut(s) 75, 114, 197, 512, 877, 1075, 1406
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 214, 324, 1132
BstHHI GCGC 1 cut(s) 942
BstKTI GATC 5 cut(s) 399, 424, 907, 1302, 1447
BstMAI GTCTC 3 cut(s) 242, 834, 1077
BstMBI GATC 5 cut(s) 396, 421, 904, 1299, 1444
BstMWI GCNNNNNNNGC 8 cut(s) 68, 375, 545, 564, 778, 887, 1077, 1331
BstNI CCWGG 2 cut(s) 228, 895
BstNSI RCATGY 2 cut(s) 478, 863
BstSCI CCNGG 2 cut(s) 226, 893
BstV1I GCAGC 3 cut(s) 83, 1014, 1553
BstX2I RGATCY 1 cut(s) 904
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 1 cut(s) 904
BstZ17I GTATAC 1 cut(s) 646
Bsu36I CCTNAGG 1 cut(s) 1075
BsuI GTATCC 2 cut(s) 297, 1247
BsuRI GGCC 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 747
BtgZI GCGATG 1 cut(s) 263
BtsCI GGATG 3 cut(s) 214, 324, 1132
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 3 cut(s) 586, 820, 1131
BveI ACCTGC 3 cut(s) 661, 864, 1236
Cac8I GCNNGC 3 cut(s) 145, 665, 861
CciI TCATGA 1 cut(s) 178
CfoI GCGC 1 cut(s) 942
Cfr13I GGNCC 1 cut(s) 853
Csp6I GTAC 1 cut(s) 1361
CspCI CAANNNNNGTGG 2 cut(s) 1049, 1084
CviQI GTAC 1 cut(s) 1361
DdeI CTNAG 7 cut(s) 75, 114, 197, 512, 877, 1075, 1406
DpnI GATC 5 cut(s) 398, 423, 906, 1301, 1446
DpnII GATC 5 cut(s) 396, 421, 904, 1299, 1444
Eam1104I CTCTTC 1 cut(s) 840
EarI CTCTTC 1 cut(s) 840
EciI GGCGGA 1 cut(s) 1095
Eco130I CCWWGG 3 cut(s) 747, 965, 1047
Eco31I GGTCTC 1 cut(s) 1077
Eco47I GGWCC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 1533
Eco81I CCTNAGG 1 cut(s) 1075
EcoO109I RGGNCCY 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 354
EcoRII CCWGG 2 cut(s) 226, 893
EcoT14I CCWWGG 3 cut(s) 747, 965, 1047
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 3 cut(s) 747, 965, 1047
Esp3I CGTCTC 1 cut(s) 834
FaqI GGGAC 2 cut(s) 587, 866
FauI CCCGC 1 cut(s) 937
FauNDI CATATG 1 cut(s) 554
FbaI TGATCA 1 cut(s) 421
FblI GTMKAC 2 cut(s) 56, 645
Fnu4HI GCNGC 4 cut(s) 72, 891, 1003, 1542
FokI GGATG 3 cut(s) 221, 331, 1139
Fsp4HI GCNGC 4 cut(s) 72, 891, 1003, 1542
FspBI CTAG 4 cut(s) 351, 488, 1161, 1322
GlaI GCGC 1 cut(s) 941
GluI GCNGC 4 cut(s) 72, 891, 1003, 1542
GsaI CCCAGC 1 cut(s) 1463
GsuI CTGGAG 3 cut(s) 33, 273, 1173
HaeIII GGCC 1 cut(s) 893
HhaI GCGC 1 cut(s) 942
Hin6I GCGC 1 cut(s) 940
HinP1I GCGC 1 cut(s) 940
HincII GTYRAC 2 cut(s) 757, 1108
HindII GTYRAC 2 cut(s) 757, 1108
HinfI GANTC 3 cut(s) 873, 1020, 1294
HphI GGTGA 3 cut(s) 455, 592, 1254
Hpy166II GTNNAC 5 cut(s) 57, 518, 646, 757, 1108
Hpy188I TCNGA 8 cut(s) 78, 162, 700, 768, 803, 1019, 1299, 1508
Hpy188III TCNNGA 5 cut(s) 106, 179, 562, 839, 1124
Hpy8I GTNNAC 5 cut(s) 57, 518, 646, 757, 1108
HpyAV CCTTC 4 cut(s) 161, 607, 1488, 1588
HpyCH4III ACNGT 1 cut(s) 1055
HpyCH4IV ACGT 2 cut(s) 1104, 1363
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 375, 545, 564, 778, 887, 1077, 1331
HpyF3I CTNAG 7 cut(s) 75, 114, 197, 512, 877, 1075, 1406
HpySE526I ACGT 2 cut(s) 1104, 1363
HspAI GCGC 1 cut(s) 940
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 5 cut(s) 396, 421, 904, 1299, 1444
LmnI GCTCC 2 cut(s) 140, 1380
Lsp1109I GCAGC 3 cut(s) 83, 1014, 1553
LweI GCATC 1 cut(s) 481
MaeI CTAG 4 cut(s) 351, 488, 1161, 1322
MaeII ACGT 2 cut(s) 1104, 1363
MaeIII GTNAC 6 cut(s) 454, 818, 1100, 1240, 1517, 1553
MalI GATC 5 cut(s) 398, 423, 906, 1301, 1446
MboI GATC 5 cut(s) 396, 421, 904, 1299, 1444
MboII GAAGA 8 cut(s) 107, 110, 194, 542, 827, 1204, 1539, 1541
MfeI CAATTG 1 cut(s) 1583
MflI RGATCY 1 cut(s) 904
MlyI GAGTC 2 cut(s) 882, 1014
MmeI TCCRAC 3 cut(s) 781, 882, 1584
Mph1103I ATGCAT 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 834
MseI TTAA 3 cut(s) 734, 995, 1209
MslI CAYNNNNRTG 4 cut(s) 183, 468, 719, 825
MspA1I CMGCKG 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 1208
MspR9I CCNGG 2 cut(s) 228, 895
MunI CAATTG 1 cut(s) 1583
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 2 cut(s) 228, 895
MwoI GCNNNNNNNGC 8 cut(s) 68, 375, 545, 564, 778, 887, 1077, 1331
NcoI CCATGG 1 cut(s) 747
NdeI CATATG 1 cut(s) 554
NdeII GATC 5 cut(s) 396, 421, 904, 1299, 1444
NlaIV GGNNCC 2 cut(s) 854, 906
NmeAIII GCCGAG 1 cut(s) 354
NmuCI GTSAC 4 cut(s) 454, 818, 1517, 1553
NsiI ATGCAT 1 cut(s) 328
NspI RCATGY 2 cut(s) 478, 863
PaeI GCATGC 1 cut(s) 863
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 661
PctI GAATGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 834
PfeI GAWTC 1 cut(s) 1294
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 4 cut(s) 73, 892, 1004, 1543
PleI GAGTC 2 cut(s) 881, 1014
PpsI GAGTC 2 cut(s) 881, 1014
Ppu21I YACGTR 1 cut(s) 1364
PpuMI RGGWCCY 1 cut(s) 853
PsiI TTATAA 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 2 cut(s) 226, 893
PspFI CCCAGC 1 cut(s) 1459
PspGI CCWGG 2 cut(s) 226, 893
PspN4I GGNNCC 2 cut(s) 854, 906
PspPI GGNCC 1 cut(s) 853
PspPPI RGGWCCY 1 cut(s) 853
PsuI RGATCY 1 cut(s) 904
RsaI GTAC 1 cut(s) 1362
RsaNI GTAC 1 cut(s) 1361
RseI CAYNNNNRTG 4 cut(s) 183, 468, 719, 825
SaqAI TTAA 3 cut(s) 734, 995, 1209
SatI GCNGC 4 cut(s) 72, 891, 1003, 1542
Sau3AI GATC 5 cut(s) 396, 421, 904, 1299, 1444
Sau96I GGNCC 1 cut(s) 853
SchI GAGTC 2 cut(s) 882, 1014
ScrFI CCNGG 2 cut(s) 228, 895
SfaNI GCATC 1 cut(s) 481
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 4 cut(s) 183, 468, 719, 825
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
SphI GCATGC 1 cut(s) 863
SsiI CCGC 5 cut(s) 60, 752, 890, 930, 1080
SspMI CTAG 4 cut(s) 351, 488, 1161, 1322
StyD4I CCNGG 2 cut(s) 226, 893
StyI CCWWGG 3 cut(s) 747, 965, 1047
TaaI ACNGT 1 cut(s) 1055
TaiI ACGT 2 cut(s) 1107, 1366
TaqI TCGA 1 cut(s) 838
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 1 cut(s) 1294
Tru1I TTAA 3 cut(s) 734, 995, 1209
Tru9I TTAA 3 cut(s) 734, 995, 1209
TscAI CASTG 3 cut(s) 593, 827, 1138
TseFI GTSAC 4 cut(s) 454, 818, 1517, 1553
TseI GCWGC 3 cut(s) 71, 1002, 1541
Tsp45I GTSAC 4 cut(s) 454, 818, 1517, 1553
TspRI CASTG 3 cut(s) 593, 827, 1138
Van91I CCANNNNNTGG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 1208
VpaK11BI GGWCC 1 cut(s) 853
XapI RAATTY 4 cut(s) 5, 354, 533, 1424
XceI RCATGY 2 cut(s) 478, 863
XmiI GTMKAC 2 cut(s) 56, 645
XmnI GAANNNNTTC 1 cut(s) 834
XspI CTAG 4 cut(s) 351, 488, 1161, 1322
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.