AT1G63180

Bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012053
Physical Location & Seq
Reverse (-)
23427405 .. 23429652
2248 bp
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UTR
Exon/CDS
Intron
AT1G63180.1

Sequence Viewer

Length: 1056 bp
ATGGGTTCTTCTGTGGAACAGAACATTTTGGTGACTGGTGGTGCTGGATTCATTGGAACACATACTGTTGTTCAGCTTTTGAATCAGGGTTTTAAGGTTACGATCATTGATAATCTTGATAACTCTGTCGTTGAAGCTGTTCATAGGGTTAGGGAACTTGTTGGTCCTGATCTCTCTACCAAGCTTGAATTCAATCTGGGTGATCTAAGAAACAAAGGAGATATTGAGAAACTCTTTTCCAATCAGAGATTTGATGCTGTGATTCACTTTGCTGGTCTTAAAGCTGTGGGAGAAAGTGTTGGAAACCCTCGTCGTTACTTTGATAATAACTTAGTTGGAACTATCAATCTATATGAGACCATGGCAAAATACAACTGCAAAATGATGGTGTTTTCGTCGTCTGCAACAGTTTATGGTCAACCTGAAATAGTCCCATGTGTGGAAGACTTTGAGTTACAGGCTATGAATCCTTATGGTCGTACTAAGCTGTTTCTTGAAGAGATAGCTAGAGACATTCACGCTGCGGAACCAGAATGGAAGATAATTCTTCTGAGGTACTTCAATCCGGTTGGAGCTCACGAGAGTGGAAGAATTGGAGAAGATCCAAAGGGCATACCGAATAATCTCATGCCTTATATCCAACAAGTGGCGGTTGGAAGATTACCTGAACTCAATGTATTTGGACATGATTATCCTACCATGGATGGTAGCGCGGTTAGAGACTACATCCATGTAATGGATTTAGCAGATGGCCATGTGGCTGCGTTAAACAAATTGTTTTCAGACTCAAAGATTGGCTGTACTGCTTATAATCTTGGCACTGGTCAAGGAACTTCTGTCTTAGAAATGGTTTCTTCTTTTGAAAAAGCTTCTGGCAAGAAAATACCTATCAAGCTATGTCCAAGAAGAGCTGGAGATGCAACAGCTGTTTATGCTTCAACTCAGAAAGCTGAGAAGGAACTTGGCTGGAAGGCAAAATATGGAGTTGATGAGATGTGCAGAGATCAATGGAACTGGGCAAATAAGAATCCATGGGGTTTCCAGAAGAAGCCTTGA

Protein Analysis

351

Amino Acids

38.91

Weight (kDa)

6.27

Isoelectric Point (pI)

24.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 8 - 171 1.2e-08 RmlD substrate binding domain
adh_short PF00106 8 - 124 2.1e-07 short chain dehydrogenase
Epimerase PF01370 9 - 264 8.4e-54 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 191 6.7e-15 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 335 3.8e-64 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 10 - 162 2.3e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12780 AT1G63180 AT1G63180
fragaria_vesca FvH4_5g01780 FvH4_5g01780
malus_domestica MD04G1064600.v1.1
prunus_persica Prupe.5G093300_v2.0.a1 Prupe.5G093300_v2.0.a1
pyrus_communis pycom04g05840
rosa_chinensis RchiOBHm_Chr7g0198901
rosa_laevigata RLG00000003834
rosa_multiflora Rmu_sc0016854.1_g000005
rosa_roxburghii Rroxscaffold_3G00257320
rosa_rugosa Rorug07G0050500 Rorug07G0050600
rosa_samantha Rh7BG177400 Rh7CG183400 Rh7DG176800
rosa_wichuraiana Rw7G015260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AccB7I CCANNNNNTGG 2 cut(s) 646, 736
AccII CGCG 1 cut(s) 713
AciI CCGC 3 cut(s) 524, 650, 713
AclWI GGATC 1 cut(s) 596
AcoI YGGCCR 1 cut(s) 751
AcsI RAATTY 1 cut(s) 188
AfaI GTAC 3 cut(s) 481, 557, 802
AfiI CCNNNNNNNGG 3 cut(s) 439, 646, 736
AgsI TTSAA 8 cut(s) 82, 134, 188, 193, 497, 562, 863, 939
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AleI CACNNNNGTG 1 cut(s) 582
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 3 cut(s) 350, 504, 714
AlwI GGATC 1 cut(s) 596
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 2 cut(s) 521, 761
ApoI RAATTY 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 43, 212
AvaII GGWCC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 753
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 508, 748
BccI CCATC 3 cut(s) 379, 698, 743
BcoDI GTCTC 3 cut(s) 350, 504, 714
BfaI CTAG 1 cut(s) 507
BisI GCNGC 2 cut(s) 522, 762
BlsI GCNGC 2 cut(s) 523, 763
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 528
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 2 cut(s) 244, 907
BmuI ACTGGG 1 cut(s) 1024
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 933
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 360, 699, 1031
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 3 cut(s) 439, 646, 736
Bse1I ACTGG 3 cut(s) 40, 826, 1019
BseDI CCNNGG 3 cut(s) 360, 699, 1031
BseGI GGATG 2 cut(s) 709, 726
BseLI CCNNNNNNNGG 3 cut(s) 439, 646, 736
BseMII CTCAG 3 cut(s) 542, 942, 956
BseNI ACTGG 3 cut(s) 40, 826, 1019
BseXI GCAGC 2 cut(s) 508, 748
BsgI GTGCAG 1 cut(s) 1018
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 566
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 3 cut(s) 439, 646, 736
BsmAI GTCTC 3 cut(s) 350, 504, 714
BsmFI GGGAC 1 cut(s) 416
BsnI GGCC 1 cut(s) 753
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 5 cut(s) 102, 169, 202, 601, 1003
Bsp19I CCATGG 3 cut(s) 360, 699, 1031
BspACI CCGC 3 cut(s) 524, 650, 713
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 3 cut(s) 543, 943, 955
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 1 cut(s) 528
BspPI GGATC 1 cut(s) 596
BspQI GCTCTTC 1 cut(s) 901
BspTNI GGTCTC 1 cut(s) 350
BsrI ACTGG 3 cut(s) 40, 826, 1019
BssECI CCNNGG 3 cut(s) 360, 699, 1031
BssMI GATC 5 cut(s) 102, 169, 202, 601, 1003
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 3 cut(s) 360, 699, 1031
Bst2BI CACGAG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 67, 409
Bst6I CTCTTC 2 cut(s) 492, 901
BstDEI CTNAG 7 cut(s) 206, 331, 483, 551, 841, 942, 951
BstDSI CCRYGG 3 cut(s) 360, 699, 1031
BstF5I GGATG 2 cut(s) 709, 726
BstFNI CGCG 1 cut(s) 713
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 5 cut(s) 105, 172, 205, 604, 1006
BstMAI GTCTC 3 cut(s) 350, 504, 714
BstMBI GATC 5 cut(s) 102, 169, 202, 601, 1003
BstMWI GCNNNNNNNGC 2 cut(s) 917, 932
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 2 cut(s) 508, 748
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 1 cut(s) 753
BtgI CCRYGG 3 cut(s) 360, 699, 1031
BtsCI GGATG 2 cut(s) 709, 726
BtsIMutI CAGTG 1 cut(s) 819
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 480, 556, 801
CviAII CATG 8 cut(s) 361, 435, 628, 686, 700, 731, 755, 1032
CviQI GTAC 3 cut(s) 480, 556, 801
DdeI CTNAG 7 cut(s) 206, 331, 483, 551, 841, 942, 951
DpnI GATC 5 cut(s) 104, 171, 204, 603, 1005
DpnII GATC 5 cut(s) 102, 169, 202, 601, 1003
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 2 cut(s) 492, 901
EarI CTCTTC 2 cut(s) 492, 901
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 360, 699, 1031
Eco24I GRGCYC 1 cut(s) 577
Eco31I GGTCTC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 575
EcoICRI GAGCTC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 188
EcoT14I CCWWGG 3 cut(s) 360, 699, 1031
EcoT38I GRGCYC 1 cut(s) 577
ErhI CCWWGG 3 cut(s) 360, 699, 1031
FaeI CATG 8 cut(s) 364, 438, 631, 689, 703, 734, 758, 1035
FaqI GGGAC 1 cut(s) 416
FatI CATG 8 cut(s) 360, 434, 627, 685, 699, 730, 754, 1031
Fnu4HI GCNGC 2 cut(s) 522, 762
FokI GGATG 2 cut(s) 713, 716
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 2 cut(s) 522, 762
FspBI CTAG 1 cut(s) 507
GlaI GCGC 1 cut(s) 712
GluI GCNGC 2 cut(s) 522, 762
GsuI CTGGAG 1 cut(s) 933
HaeIII GGCC 1 cut(s) 753
HapII CCGG 1 cut(s) 566
HhaI GCGC 1 cut(s) 713
Hin1II CATG 8 cut(s) 364, 438, 631, 689, 703, 734, 758, 1035
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HincII GTYRAC 1 cut(s) 419
HindII GTYRAC 1 cut(s) 419
HindIII AAGCTT 2 cut(s) 182, 867
HinfI GANTC 6 cut(s) 48, 82, 262, 466, 785, 1027
HpaII CCGG 1 cut(s) 566
HphI GGTGA 2 cut(s) 43, 212
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 4 cut(s) 246, 552, 784, 945
Hpy188III TCNNGA 5 cut(s) 116, 167, 494, 578, 1042
Hpy8I GTNNAC 1 cut(s) 419
Hpy99I CGWCG 2 cut(s) 315, 400
HpyAV CCTTC 2 cut(s) 949, 964
HpyCH4III ACNGT 2 cut(s) 67, 409
HpyCH4V TGCA 4 cut(s) 378, 404, 920, 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 917, 932
HpyF3I CTNAG 7 cut(s) 206, 331, 483, 551, 841, 942, 951
Hsp92II CATG 8 cut(s) 364, 438, 631, 689, 703, 734, 758, 1035
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 5 cut(s) 102, 169, 202, 601, 1003
LguI GCTCTTC 1 cut(s) 901
LmnI GCTCC 1 cut(s) 572
Lsp1109I GCAGC 2 cut(s) 508, 748
LweI GCATC 2 cut(s) 244, 907
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 4 cut(s) 31, 97, 314, 453
MalI GATC 5 cut(s) 104, 171, 204, 603, 1005
MboI GATC 5 cut(s) 102, 169, 202, 601, 1003
MboII GAAGA 9 cut(s) 455, 509, 539, 550, 600, 611, 669, 846, 918
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 4 cut(s) 188, 543, 591, 773
MluNI TGGCCA 1 cut(s) 753
MlyI GAGTC 1 cut(s) 779
MmeI TCCRAC 5 cut(s) 280, 316, 550, 634, 664
MnlI CCTC 2 cut(s) 318, 546
Mox20I TGGCCA 1 cut(s) 753
MroXI GAANNNNTTC 1 cut(s) 138
MscI TGGCCA 1 cut(s) 753
MseI TTAA 3 cut(s) 93, 279, 767
MslI CAYNNNNRTG 2 cut(s) 29, 582
Msp20I TGGCCA 1 cut(s) 753
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 1 cut(s) 566
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 2 cut(s) 917, 932
NcoI CCATGG 3 cut(s) 360, 699, 1031
NdeII GATC 5 cut(s) 102, 169, 202, 601, 1003
NlaIII CATG 8 cut(s) 364, 438, 631, 689, 703, 734, 758, 1035
NlaIV GGNNCC 1 cut(s) 528
NmuCI GTSAC 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 901
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 5 cut(s) 48, 82, 262, 466, 1027
PflMI CCANNNNNTGG 2 cut(s) 646, 736
PkrI GCNGC 2 cut(s) 523, 763
PleI GAGTC 1 cut(s) 779
PpsI GAGTC 1 cut(s) 779
PsiI TTATAA 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 528
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 3 cut(s) 481, 557, 802
RsaNI GTAC 3 cut(s) 480, 556, 801
RseI CAYNNNNRTG 2 cut(s) 29, 582
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 901
SaqAI TTAA 3 cut(s) 93, 279, 767
SatI GCNGC 2 cut(s) 522, 762
Sau3AI GATC 5 cut(s) 102, 169, 202, 601, 1003
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 1 cut(s) 779
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 2 cut(s) 244, 907
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 2 cut(s) 29, 582
Sse9I AATT 4 cut(s) 188, 543, 591, 773
SsiI CCGC 3 cut(s) 524, 650, 713
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 577
StyI CCWWGG 3 cut(s) 360, 699, 1031
TaaI ACNGT 2 cut(s) 67, 409
TasI AATT 4 cut(s) 188, 543, 591, 773
TatI WGTACW 1 cut(s) 800
TfiI GAWTC 5 cut(s) 48, 82, 262, 466, 1027
Tru1I TTAA 3 cut(s) 93, 279, 767
Tru9I TTAA 3 cut(s) 93, 279, 767
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 521, 761
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 40, 131, 479
TspRI CASTG 1 cut(s) 826
Van91I CCANNNNNTGG 2 cut(s) 646, 736
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.