MD04G1064600.v1.1

bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012053
Physical Location & Seq
Forward (+)
8687642 .. 8691096
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1064600.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGGGTCTATGGAGCAGACAATTCTGGTGACCGGCGGCGCGGGGTTCATAGGGAGCCACACGGTGATTCAGCTGCTCGAGGCGGGGTTTAGGGTTTCCGTCATTGATAATCTCGATAACTCCGTTGCTGAAGCTGTTGATCGCGTCAGGGACTTGGTCGGCCCCAAGCTCTCTCAGAAACTCCACTTTCAGCTGGGCGATCTCAGAAATAAGGACGATTTGGAGAAGCTGTTTTCTCAAACTAAGTTTGATGCTGTAATCCACTTTGCTGGCCTAAAAGCTGTAGGAGAAAGCGTTGCACACCCGCACCGTTATTATGATAACAATTTGGTTGGAACTATTAATCTTTACGCGGTCATGGCGAAATACAATTGTAAAAAGATGGTTTTTTCATCATCTGCAACTGTGTATGGGCAACCTGAAAAAATTCCATGTGTTGAGGATTTCAACCTTGTAGCTTTGAATCCATATGGCCGTACCAAGCTTTTCCTTGAAGAAATTGCTCGAGATATGCAAAATGCAGACCCAGATTGGCAAATTATTCTGCTGAGGTACTTCAATCCTGTCGGGGCTCATGAGAGTGGGAAAATTGGTGAAGATCCCAAAGGCATCCCAAACAACCTAATGCCGTACATACAGCAAGTAGCTGTTGGAAGATTGCCTGAGCTTAATGTCTATGGTCATGATTATCCCACCAAGGATGGTACTGCGATCCGAGACTACATCCATGTTATGGACTTAGCAGATGGTCATATTGCTGCTCTTCAGAAGCTTTTTACTACAGAGAATATAGGTTGTGCTGCCTACAATCTGGGAACCGGACAAGGTACATCGGTGCTTGAAATGGTTGCTGGCTTCGAGAAGGCTTCTGGCAAGAAAATTCCTATCAAACTATGTCCCAGGAGGCCAGGTGATGCTACTGCTGTTTATGCTTCAACAGAGAAAGCTGAAAGGGAACTCGGGTGGAAGGCAAAATACGGAATAGAGGAGATGTGCAGGGACCAATGGAAGTGGGCAGTAAACAATCCGCACGGTTACAAGTCGAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00520 ko01100 map00052 map00520 map01100
Pfam Domains
Protein Families
GDP_Man_Dehyd

Protein Analysis

351

Amino Acids

38.83

Weight (kDa)

6.56

Isoelectric Point (pI)

24.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 6 - 123 9.3e-09 short chain dehydrogenase
RmlD_sub_bind PF04321 7 - 170 3.5e-09 RmlD substrate binding domain
Epimerase PF01370 8 - 262 2.1e-56 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 190 1.3e-15 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 9 - 334 4.2e-67 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 9 - 139 1.3e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 181 4.1e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12780 AT1G63180 AT1G63180
fragaria_vesca FvH4_5g01780 FvH4_5g01780
malus_domestica MD04G1064600.v1.1
prunus_persica Prupe.5G093300_v2.0.a1 Prupe.5G093300_v2.0.a1
pyrus_communis pycom04g05840
rosa_chinensis RchiOBHm_Chr7g0198901
rosa_laevigata RLG00000003834
rosa_multiflora Rmu_sc0016854.1_g000005
rosa_roxburghii Rroxscaffold_3G00257320
rosa_rugosa Rorug07G0050500 Rorug07G0050600
rosa_samantha Rh7BG177400 Rh7CG183400 Rh7DG176800
rosa_wichuraiana Rw7G015260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 733
AccII CGCG 3 cut(s) 41, 144, 353
AciI CCGC 6 cut(s) 36, 41, 83, 305, 353, 1028
AclWI GGATC 2 cut(s) 593, 706
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 2 cut(s) 426, 879
AcuI CTGAAG 2 cut(s) 150, 749
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 5 cut(s) 478, 554, 632, 706, 829
AfiI CCNNNNNNNGG 1 cut(s) 733
AgsI TTSAA 6 cut(s) 448, 463, 494, 559, 842, 936
AjnI CCWGG 2 cut(s) 899, 907
Alw26I GTCTC 1 cut(s) 711
AlwI GGATC 2 cut(s) 593, 706
Ama87I CYCGRG 3 cut(s) 77, 504, 959
AoxI GGCC 4 cut(s) 160, 271, 472, 905
ApeKI GCWGC 3 cut(s) 73, 758, 800
ApoI RAATTY 2 cut(s) 426, 879
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 426
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 161, 1000
AsuHPI GGTGA 4 cut(s) 40, 76, 605, 923
AvaI CYCGRG 3 cut(s) 77, 504, 959
AvaII GGWCC 1 cut(s) 1000
BaeI ACNNNNGTAYC 2 cut(s) 819, 852
BanII GRGCYC 1 cut(s) 574
BarI GAAGNNNNNNTAC 2 cut(s) 959, 991
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 3 cut(s) 60, 745, 787
BccI CCATC 3 cut(s) 376, 695, 740
BceAI ACGGC 2 cut(s) 459, 613
BciT130I CCWGG 2 cut(s) 901, 909
BcoDI GTCTC 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 282, 780
BisI GCNGC 4 cut(s) 37, 74, 759, 801
BlsI GCNGC 4 cut(s) 38, 75, 760, 802
Bme1390I CCNGG 2 cut(s) 901, 909
Bme18I GGWCC 1 cut(s) 1000
BmeT110I CYCGRG 3 cut(s) 77, 504, 959
BmgT120I GGNCC 2 cut(s) 161, 1000
BmiI GGNNCC 4 cut(s) 56, 163, 817, 1001
BmrFI CCNGG 2 cut(s) 901, 909
BmsI GCATC 3 cut(s) 241, 618, 904
Bpu10I CCTNAGC 2 cut(s) 548, 663
BsaJI CCNNGG 2 cut(s) 696, 899
BsaWI WCCGGW 1 cut(s) 818
BsaXI ACNNNNNCTCC 6 cut(s) 215, 245, 279, 309, 895, 925
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse118I RCCGGY 1 cut(s) 32
BseBI CCWGG 2 cut(s) 901, 909
BseDI CCNNGG 2 cut(s) 696, 899
BseGI GGATG 3 cut(s) 609, 706, 723
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMII CTCAG 4 cut(s) 188, 217, 539, 654
BseRI GAGGAG 1 cut(s) 1001
BseXI GCAGC 3 cut(s) 60, 745, 787
BseYI CCCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 1015
Bsh1236I CGCG 3 cut(s) 41, 144, 353
BshFI GGCC 4 cut(s) 162, 273, 474, 907
BsiHKCI CYCGRG 3 cut(s) 77, 504, 959
BsiSI CCGG 2 cut(s) 33, 819
BslFI GGGAC 3 cut(s) 164, 882, 1013
BslI CCNNNNNNNGG 1 cut(s) 733
BsmAI GTCTC 1 cut(s) 711
BsmFI GGGAC 3 cut(s) 164, 882, 1013
BsnI GGCC 4 cut(s) 162, 273, 474, 907
BsoBI CYCGRG 3 cut(s) 77, 504, 959
Bsp1286I GDGCHC 1 cut(s) 574
Bsp143I GATC 4 cut(s) 139, 199, 598, 711
BspACI CCGC 6 cut(s) 36, 41, 83, 305, 353, 1028
BspANI GGCC 4 cut(s) 162, 273, 474, 907
BspCNI CTCAG 4 cut(s) 187, 216, 540, 655
BspFNI CGCG 3 cut(s) 41, 144, 353
BspHI TCATGA 2 cut(s) 574, 682
BspLI GGNNCC 4 cut(s) 56, 163, 817, 1001
BspPI GGATC 2 cut(s) 593, 706
BspQI GCTCTTC 1 cut(s) 768
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 696, 899
BssMI GATC 4 cut(s) 139, 199, 598, 711
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 901, 909
Bst4CI ACNGT 4 cut(s) 64, 311, 406, 1034
Bst6I CTCTTC 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 271, 853
BstDEI CTNAG 6 cut(s) 174, 203, 243, 548, 663, 739
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 3 cut(s) 609, 706, 723
BstFNI CGCG 3 cut(s) 41, 144, 353
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 4 cut(s) 142, 202, 601, 714
BstMAI GTCTC 1 cut(s) 711
BstMBI GATC 4 cut(s) 139, 199, 598, 711
BstMWI GCNNNNNNNGC 2 cut(s) 359, 929
BstNI CCWGG 2 cut(s) 901, 909
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 2 cut(s) 899, 907
BstSFI CTRYAG 2 cut(s) 282, 780
BstUI CGCG 3 cut(s) 41, 144, 353
BstV1I GCAGC 3 cut(s) 60, 745, 787
BstX2I RGATCY 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 4 cut(s) 162, 273, 474, 907
BtsCI GGATG 3 cut(s) 609, 706, 723
Cac8I GCNNGC 2 cut(s) 271, 853
CciI TCATGA 2 cut(s) 574, 682
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 161, 1000
CseI GACGC 1 cut(s) 133
Csp6I GTAC 5 cut(s) 477, 553, 631, 705, 828
CspCI CAANNNNNGTGG 2 cut(s) 992, 1027
CviAII CATG 5 cut(s) 358, 432, 575, 683, 728
CviQI GTAC 5 cut(s) 477, 553, 631, 705, 828
DdeI CTNAG 6 cut(s) 174, 203, 243, 548, 663, 739
DpnI GATC 4 cut(s) 141, 201, 600, 713
DpnII GATC 4 cut(s) 139, 199, 598, 711
DraIII CACNNNGTG 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 1000
Eco57I CTGAAG 2 cut(s) 150, 749
Eco88I CYCGRG 3 cut(s) 77, 504, 959
Eco91I GGTNACC 1 cut(s) 28
EcoO65I GGTNACC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 899, 907
EcoT14I CCWWGG 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 5 cut(s) 361, 435, 578, 686, 731
FaqI GGGAC 3 cut(s) 164, 882, 1013
FatI CATG 5 cut(s) 357, 431, 574, 682, 727
FauI CCCGC 3 cut(s) 34, 76, 312
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 4 cut(s) 37, 74, 759, 801
FokI GGATG 3 cut(s) 596, 710, 713
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 4 cut(s) 37, 74, 759, 801
GlaI GCGC 1 cut(s) 40
GluI GCNGC 4 cut(s) 37, 74, 759, 801
GsaI CCCAGC 1 cut(s) 197
HaeIII GGCC 4 cut(s) 162, 273, 474, 907
HapII CCGG 2 cut(s) 33, 819
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 41
Hin1II CATG 5 cut(s) 361, 435, 578, 686, 731
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 2 cut(s) 482, 770
HinfI GANTC 2 cut(s) 67, 463
HpaII CCGG 2 cut(s) 33, 819
HphI GGTGA 4 cut(s) 40, 76, 605, 923
Hpy166II GTNNAC 1 cut(s) 1021
Hpy188I TCNGA 4 cut(s) 177, 206, 716, 768
Hpy188III TCNNGA 5 cut(s) 113, 506, 575, 683, 859
Hpy8I GTNNAC 1 cut(s) 1021
HpyAV CCTTC 2 cut(s) 856, 961
HpyCH4III ACNGT 4 cut(s) 64, 311, 406, 1034
HpyCH4V TGCA 5 cut(s) 299, 401, 514, 521, 996
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 929
HpyF3I CTNAG 6 cut(s) 174, 203, 243, 548, 663, 739
Hsp92II CATG 5 cut(s) 361, 435, 578, 686, 731
HspAI GCGC 1 cut(s) 39
Kzo9I GATC 4 cut(s) 139, 199, 598, 711
LguI GCTCTTC 1 cut(s) 768
LmnI GCTCC 2 cut(s) 13, 54
Lsp1109I GCAGC 3 cut(s) 60, 745, 787
LweI GCATC 3 cut(s) 241, 618, 904
MaeIII GTNAC 2 cut(s) 28, 1034
MalI GATC 4 cut(s) 141, 201, 600, 713
MboI GATC 4 cut(s) 139, 199, 598, 711
MboII GAAGA 4 cut(s) 506, 608, 666, 755
MfeI CAATTG 1 cut(s) 370
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 8 cut(s) 21, 325, 370, 426, 498, 537, 588, 879
MmeI TCCRAC 2 cut(s) 313, 631
MnlI CCTC 5 cut(s) 73, 433, 543, 897, 979
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 2 cut(s) 342, 669
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 2 cut(s) 73, 193
MspI CCGG 2 cut(s) 33, 819
MspR9I CCNGG 2 cut(s) 901, 909
MunI CAATTG 1 cut(s) 370
MvaI CCWGG 2 cut(s) 901, 909
MvnI CGCG 3 cut(s) 41, 144, 353
MwoI GCNNNNNNNGC 2 cut(s) 359, 929
NdeI CATATG 1 cut(s) 469
NdeII GATC 4 cut(s) 139, 199, 598, 711
NlaIII CATG 5 cut(s) 361, 435, 578, 686, 731
NlaIV GGNNCC 4 cut(s) 56, 163, 817, 1001
NmuCI GTSAC 1 cut(s) 28
PaeR7I CTCGAG 2 cut(s) 77, 504
PagI TCATGA 2 cut(s) 574, 682
PciSI GCTCTTC 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 67, 463
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 4 cut(s) 38, 75, 760, 802
PshBI ATTAAT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 899, 907
PspEI GGTNACC 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 899, 907
PspN4I GGNNCC 4 cut(s) 56, 163, 817, 1001
PspPI GGNCC 2 cut(s) 161, 1000
PspXI VCTCGAGB 1 cut(s) 77
PsuI RGATCY 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 2 cut(s) 73, 193
RsaI GTAC 5 cut(s) 478, 554, 632, 706, 829
RsaNI GTAC 5 cut(s) 477, 553, 631, 705, 828
RseI CAYNNNNRTG 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 768
SaqAI TTAA 2 cut(s) 342, 669
SatI GCNGC 4 cut(s) 37, 74, 759, 801
Sau3AI GATC 4 cut(s) 139, 199, 598, 711
Sau96I GGNCC 2 cut(s) 161, 1000
ScrFI CCNGG 2 cut(s) 901, 909
SduI GDGCHC 1 cut(s) 574
SfaNI GCATC 3 cut(s) 241, 618, 904
SfcI CTRYAG 2 cut(s) 282, 780
Sfr274I CTCGAG 2 cut(s) 77, 504
SinI GGWCC 1 cut(s) 1000
SlaI CTCGAG 2 cut(s) 77, 504
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 2 cut(s) 77, 504
SmoI CTYRAG 2 cut(s) 77, 504
Sse9I AATT 8 cut(s) 21, 325, 370, 426, 498, 537, 588, 879
SsiI CCGC 6 cut(s) 36, 41, 83, 305, 353, 1028
StyD4I CCNGG 2 cut(s) 899, 907
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 4 cut(s) 64, 311, 406, 1034
TaqI TCGA 5 cut(s) 78, 114, 505, 858, 1043
TasI AATT 8 cut(s) 21, 325, 370, 426, 498, 537, 588, 879
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 67, 463
Tru1I TTAA 2 cut(s) 342, 669
Tru9I TTAA 2 cut(s) 342, 669
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 73, 758, 800
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 37, 381
TspGWI ACGGA 3 cut(s) 88, 112, 993
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 1000
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 2 cut(s) 426, 879
XhoI CTCGAG 2 cut(s) 77, 504
XmnI GAANNNNTTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.