FvH4_5g01780

bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012053
Physical Location & Seq
Reverse (-)
1132143 .. 1135413
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01780.t3

Sequence Viewer

Length: 1068 bp
ATGGGGTCGATAGAGCAGACGATACTAGTGACGGGAGGCGCGGGGTTCATAGGGAGCCACACGGCGGTGCAGCTACTCAACGAGGGCTTCAGAGTCGTCATCTTTGATAATCTTGATAATTCTTGTATTGAAGCTGTTCATCGCGTCCGCGAACTCGTCGGACCCAAGCTCTCCCAGAAGCTTCAGTTTCATCTGGGTGATTTGAGAAATAAGGAGGAGTTGGACAAGCTATTTTCGCAAACTAAGTTTGATGCTGTGATCCATTTTGCTGGCTTGAAAGCTGTTGGGGAGAGTGTTGCGCACCCTCTCCGCTATTTTGACAACAATTTGATTGGCACTATTAATCTCTATGATATCATGGCAAAATACAATTGCAAAAAGATGGTTTTTTCTTCATCTGCAACTGTTTATGGCCAACCTGAAAAAATTCCTTGTGTTGAGGATTTTGACCTTGTAGCTCTGAATCCATATGGTCGTACCAAGCTTTTCCTCGAAGAAATTGCTCGAGATATTCACAGGGCAGAGCCAGATTGGGGAATTATTCTTCTGAGGTATTTCAACCCCGTGGGGGCTCATGAGAGTGGTAAAATTGGTGAAGATCCTAAAGGCATACCAAACAATCTCATGCCTTACATACAGCAAGTAGCTGTTGGAAGATTGCCTGAGCTTAATGTCTATGGTCATGACTATCCCACCAGGGATGGTACTGCGATCCGAGACTACATCCATGTGATGGACTTAGCAGATGGCCATATTGCTGCTCTTCAGAAGCTTTTTACTACAGAGAATACAGGTTGTATCGCCTACAATCTGGGAACCGGACAAGGTACATCAGTGCTCGAAATGGTTGCTGCCTTTGAAAAAGCTTCTGGGAAGAAAATCCCTATCAAACTATGTCCAAGAAGGCCAGGAGATGCTACTGCTGTTTATGCTTCAACTGAGAAAGCTAGAAGGGAACTTGGTTGGAAGGCGAAATATGGAGTGGAGGAGATGTGCAGAGACCAATGGAATTGGGCAGTAAACAATCCATGGGGGTACGTTGCCCCGTCCAAGGACTCCAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00520 ko01100 map00052 map00520 map01100
Pfam Domains
Protein Families
GDP_Man_Dehyd

Protein Analysis

356

Amino Acids

39.5

Weight (kDa)

6.56

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 6 - 121 5e-08 short chain dehydrogenase
RmlD_sub_bind PF04321 7 - 170 9.4e-09 RmlD substrate binding domain
Epimerase PF01370 8 - 272 6e-56 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 133 6.2e-17 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 9 - 248 1.4e-56 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 9 - 139 4.5e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12780 AT1G63180 AT1G63180
fragaria_vesca FvH4_5g01780 FvH4_5g01780
malus_domestica MD04G1064600.v1.1
prunus_persica Prupe.5G093300_v2.0.a1 Prupe.5G093300_v2.0.a1
pyrus_communis pycom04g05840
rosa_chinensis RchiOBHm_Chr7g0198901
rosa_laevigata RLG00000003834
rosa_multiflora Rmu_sc0016854.1_g000005
rosa_roxburghii Rroxscaffold_3G00257320
rosa_rugosa Rorug07G0050500 Rorug07G0050600
rosa_samantha Rh7BG177400 Rh7CG183400 Rh7DG176800
rosa_wichuraiana Rw7G015260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 300
AccB7I CCANNNNNTGG 1 cut(s) 733
AccII CGCG 3 cut(s) 41, 144, 150
AciI CCGC 4 cut(s) 41, 65, 148, 310
AclWI GGATC 3 cut(s) 253, 593, 706
AcoI YGGCCR 2 cut(s) 412, 748
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 3 cut(s) 73, 167, 749
AfaI GTAC 4 cut(s) 478, 706, 829, 1037
AfiI CCNNNNNNNGG 5 cut(s) 64, 533, 568, 733, 1051
AgsI TTSAA 5 cut(s) 131, 277, 559, 860, 936
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 695, 907
AleI CACNNNNGTG 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 711, 993
AlwI GGATC 3 cut(s) 253, 593, 706
Ama87I CYCGRG 1 cut(s) 504
AoxI GGCC 3 cut(s) 412, 748, 905
ApeKI GCWGC 3 cut(s) 70, 758, 851
ApoI RAATTY 1 cut(s) 426
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 2 cut(s) 135, 426
AspLEI GCGC 2 cut(s) 41, 301
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 209, 605
AvaI CYCGRG 1 cut(s) 504
AvaII GGWCC 1 cut(s) 161
BaeI ACNNNNGTAYC 2 cut(s) 819, 852
BalI TGGCCA 2 cut(s) 414, 750
BanII GRGCYC 1 cut(s) 574
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 3 cut(s) 82, 745, 838
BccI CCATC 4 cut(s) 376, 695, 727, 740
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 4 cut(s) 482, 516, 830, 864
BciT130I CCWGG 2 cut(s) 697, 909
BcoDI GTCTC 2 cut(s) 711, 993
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 2 cut(s) 26, 948
BfmI CTRYAG 1 cut(s) 780
BisI GCNGC 3 cut(s) 71, 759, 852
BlsI GCNGC 3 cut(s) 72, 760, 853
Bme1390I CCNGG 2 cut(s) 697, 909
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 3 cut(s) 56, 163, 817
BmrFI CCNGG 2 cut(s) 697, 909
BmsI GCATC 2 cut(s) 241, 904
Bpu10I CCTNAGC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 993
BsaJI CCNNGG 4 cut(s) 564, 696, 1028, 1050
BsaWI WCCGGW 1 cut(s) 818
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 533, 568, 733, 1051
BseBI CCWGG 2 cut(s) 697, 909
BseDI CCNNGG 4 cut(s) 564, 696, 1028, 1050
BseGI GGATG 2 cut(s) 706, 723
BseLI CCNNNNNNNGG 5 cut(s) 64, 533, 568, 733, 1051
BseMII CTCAG 3 cut(s) 539, 654, 930
BseRI GAGGAG 2 cut(s) 230, 1001
BseXI GCAGC 3 cut(s) 82, 745, 838
BsgI GTGCAG 2 cut(s) 89, 1015
Bsh1236I CGCG 3 cut(s) 41, 144, 150
BshFI GGCC 3 cut(s) 414, 750, 907
BsiHKAI GWGCWC 1 cut(s) 840
BsiHKCI CYCGRG 1 cut(s) 504
BsiSI CCGG 1 cut(s) 819
BslI CCNNNNNNNGG 5 cut(s) 64, 533, 568, 733, 1051
BsmAI GTCTC 2 cut(s) 711, 993
BsnI GGCC 3 cut(s) 414, 750, 907
Bso31I GGTCTC 1 cut(s) 993
BsoBI CYCGRG 1 cut(s) 504
Bsp1286I GDGCHC 2 cut(s) 574, 840
Bsp143I GATC 3 cut(s) 258, 598, 711
Bsp19I CCATGG 1 cut(s) 1028
BspACI CCGC 4 cut(s) 41, 65, 148, 310
BspANI GGCC 3 cut(s) 414, 750, 907
BspCNI CTCAG 3 cut(s) 540, 655, 931
BspFNI CGCG 3 cut(s) 41, 144, 150
BspHI TCATGA 2 cut(s) 574, 682
BspLI GGNNCC 3 cut(s) 56, 163, 817
BspPI GGATC 3 cut(s) 253, 593, 706
BspQI GCTCTTC 1 cut(s) 768
BspTNI GGTCTC 1 cut(s) 993
BssECI CCNNGG 4 cut(s) 564, 696, 1028, 1050
BssMI GATC 3 cut(s) 258, 598, 711
BssT1I CCWWGG 2 cut(s) 1028, 1050
Bst2UI CCWGG 2 cut(s) 697, 909
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 5 cut(s) 243, 548, 663, 739, 939
BstDSI CCRYGG 2 cut(s) 564, 1028
BstF5I GGATG 2 cut(s) 706, 723
BstFNI CGCG 3 cut(s) 41, 144, 150
BstHHI GCGC 2 cut(s) 41, 301
BstKTI GATC 3 cut(s) 261, 601, 714
BstMAI GTCTC 2 cut(s) 711, 993
BstMBI GATC 3 cut(s) 258, 598, 711
BstMWI GCNNNNNNNGC 2 cut(s) 235, 929
BstNI CCWGG 2 cut(s) 697, 909
BstSCI CCNGG 2 cut(s) 695, 907
BstSFI CTRYAG 1 cut(s) 780
BstUI CGCG 3 cut(s) 41, 144, 150
BstV1I GCAGC 3 cut(s) 82, 745, 838
BstX2I RGATCY 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 3 cut(s) 414, 750, 907
BtgI CCRYGG 2 cut(s) 564, 1028
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 2 cut(s) 706, 723
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 1 cut(s) 271
CciI TCATGA 2 cut(s) 574, 682
CfoI GCGC 2 cut(s) 41, 301
Cfr13I GGNCC 1 cut(s) 161
CseI GACGC 1 cut(s) 133
Csp6I GTAC 4 cut(s) 477, 705, 828, 1036
CviAII CATG 6 cut(s) 358, 575, 625, 683, 728, 1029
CviQI GTAC 4 cut(s) 477, 705, 828, 1036
DdeI CTNAG 5 cut(s) 243, 548, 663, 739, 939
DpnI GATC 3 cut(s) 260, 600, 713
DpnII GATC 3 cut(s) 258, 598, 711
EaeI YGGCCR 2 cut(s) 412, 748
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 1028, 1050
Eco24I GRGCYC 1 cut(s) 574
Eco31I GGTCTC 1 cut(s) 993
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 3 cut(s) 73, 167, 749
Eco88I CYCGRG 1 cut(s) 504
EcoRII CCWGG 2 cut(s) 695, 907
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 2 cut(s) 1028, 1050
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 2 cut(s) 1028, 1050
FaeI CATG 6 cut(s) 361, 578, 628, 686, 731, 1032
FatI CATG 6 cut(s) 357, 574, 624, 682, 727, 1028
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 3 cut(s) 71, 759, 852
FokI GGATG 2 cut(s) 710, 713
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 3 cut(s) 71, 759, 852
FspBI CTAG 2 cut(s) 26, 948
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 2 cut(s) 40, 300
GluI GCNGC 3 cut(s) 71, 759, 852
HaeIII GGCC 3 cut(s) 414, 750, 907
HapII CCGG 1 cut(s) 819
HgaI GACGC 1 cut(s) 133
HhaI GCGC 2 cut(s) 41, 301
Hin1II CATG 6 cut(s) 361, 578, 628, 686, 731, 1032
Hin6I GCGC 2 cut(s) 39, 299
HinP1I GCGC 2 cut(s) 39, 299
HindIII AAGCTT 4 cut(s) 179, 482, 770, 864
HinfI GANTC 3 cut(s) 93, 463, 1055
HpaII CCGG 1 cut(s) 819
HphI GGTGA 2 cut(s) 209, 605
Hpy166II GTNNAC 1 cut(s) 1021
Hpy188I TCNGA 6 cut(s) 92, 161, 462, 549, 716, 768
Hpy188III TCNNGA 4 cut(s) 113, 506, 575, 683
Hpy8I GTNNAC 1 cut(s) 1021
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 897, 945, 961
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 1 cut(s) 1038
HpyCH4V TGCA 4 cut(s) 70, 375, 401, 996
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 929
HpyF3I CTNAG 5 cut(s) 243, 548, 663, 739, 939
HpySE526I ACGT 1 cut(s) 1038
Hsp92II CATG 6 cut(s) 361, 578, 628, 686, 731, 1032
HspAI GCGC 2 cut(s) 39, 299
Kzo9I GATC 3 cut(s) 258, 598, 711
LguI GCTCTTC 1 cut(s) 768
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 3 cut(s) 82, 745, 838
LweI GCATC 2 cut(s) 241, 904
MaeI CTAG 2 cut(s) 26, 948
MaeII ACGT 1 cut(s) 1038
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 260, 600, 713
MboI GATC 3 cut(s) 258, 598, 711
MboII GAAGA 7 cut(s) 384, 506, 536, 608, 666, 755, 886
MfeI CAATTG 1 cut(s) 370
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 2 cut(s) 574, 840
MlsI TGGCCA 2 cut(s) 414, 750
MluCI AATT 8 cut(s) 118, 325, 370, 426, 498, 537, 588, 1009
MluNI TGGCCA 2 cut(s) 414, 750
MlyI GAGTC 2 cut(s) 102, 1049
MmeI TCCRAC 4 cut(s) 139, 201, 631, 944
MnlI CCTC 8 cut(s) 29, 76, 208, 315, 433, 500, 543, 979
Mox20I TGGCCA 2 cut(s) 414, 750
MroXI GAANNNNTTC 2 cut(s) 135, 426
MscI TGGCCA 2 cut(s) 414, 750
MseI TTAA 2 cut(s) 342, 669
MslI CAYNNNNRTG 4 cut(s) 65, 195, 579, 728
Msp20I TGGCCA 2 cut(s) 414, 750
MspI CCGG 1 cut(s) 819
MspR9I CCNGG 2 cut(s) 697, 909
MunI CAATTG 1 cut(s) 370
MvaI CCWGG 2 cut(s) 697, 909
MvnI CGCG 3 cut(s) 41, 144, 150
MwoI GCNNNNNNNGC 2 cut(s) 235, 929
NcoI CCATGG 1 cut(s) 1028
NdeI CATATG 1 cut(s) 469
NdeII GATC 3 cut(s) 258, 598, 711
NlaIII CATG 6 cut(s) 361, 578, 628, 686, 731, 1032
NlaIV GGNNCC 3 cut(s) 56, 163, 817
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 504
PagI TCATGA 2 cut(s) 574, 682
PciSI GCTCTTC 1 cut(s) 768
PdmI GAANNNNTTC 2 cut(s) 135, 426
PfeI GAWTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 3 cut(s) 72, 760, 853
PleI GAGTC 2 cut(s) 101, 1049
PpsI GAGTC 2 cut(s) 101, 1049
PshBI ATTAAT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 695, 907
PspGI CCWGG 2 cut(s) 695, 907
PspN4I GGNNCC 3 cut(s) 56, 163, 817
PspPI GGNCC 1 cut(s) 161
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 4 cut(s) 478, 706, 829, 1037
RsaNI GTAC 4 cut(s) 477, 705, 828, 1036
RseI CAYNNNNRTG 4 cut(s) 65, 195, 579, 728
SapI GCTCTTC 1 cut(s) 768
SaqAI TTAA 2 cut(s) 342, 669
SatI GCNGC 3 cut(s) 71, 759, 852
Sau3AI GATC 3 cut(s) 258, 598, 711
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 2 cut(s) 102, 1049
ScrFI CCNGG 2 cut(s) 697, 909
SduI GDGCHC 2 cut(s) 574, 840
SfaNI GCATC 2 cut(s) 241, 904
SfcI CTRYAG 1 cut(s) 780
Sfr274I CTCGAG 1 cut(s) 504
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 504
SmiMI CAYNNNNRTG 4 cut(s) 65, 195, 579, 728
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 8 cut(s) 118, 325, 370, 426, 498, 537, 588, 1009
SsiI CCGC 4 cut(s) 41, 65, 148, 310
SspMI CTAG 2 cut(s) 26, 948
StyD4I CCNGG 2 cut(s) 695, 907
StyI CCWWGG 2 cut(s) 1028, 1050
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 1 cut(s) 1041
TaqI TCGA 4 cut(s) 8, 492, 505, 840
TasI AATT 8 cut(s) 118, 325, 370, 426, 498, 537, 588, 1009
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 2 cut(s) 342, 669
Tru9I TTAA 2 cut(s) 342, 669
TscAI CASTG 1 cut(s) 840
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 70, 758, 851
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 4 cut(s) 37, 128, 179, 384
TspRI CASTG 1 cut(s) 840
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 161
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 1 cut(s) 426
XhoI CTCGAG 1 cut(s) 504
XmnI GAANNNNTTC 2 cut(s) 135, 426
XspI CTAG 2 cut(s) 26, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.