AT1G67820

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012422
Physical Location & Seq
Forward (+)
25429750 .. 25431600
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67820.2

Sequence Viewer

Length: 1338 bp
ATGACTAATAAACTTCGCTCAGAAGAAACAATCACGTCCCTTTCTTCTTTCATGGCTAGTACACTTAGCATTGCGTCACCCTCTCCTTGTTCTATTCCATTGTCGGTCACAAAGGTCTCGCCTTTAAAGAGAAAGAGACCTACCCACTTAAATATCCCTGACCTCAATCCTCAACAACCGATTTCAACTGATTACTTCCGTTTCAGAGAGGGTGACGCAAAGGTCTCGCCTTTAAAGAGAAAGAGACCTGCCCACCTAAATATCCCTGACCTCAATCCTCAACAACCGATTCGAACTGATTACTTCAGTTTCACAGACTTTGCTCACCAAAACGGCACCGTAAGCTTCGGTGGCAATGGTTTTGGTGTCGTCTCCAGAAACGGTAAGAAGAAGTTTATGGAAGATACTCACAGAATTGTTCCTTGTTTGGTTGGTAACTCCAAAAAGAGTTTCTTTGGAGTTTATGATGGTCATGGAGGTGCTAAAGCTGCAGAATTTGTGGCTGAGAATTTGCACAAATATGTTGTTGAGATGATGGAGAATTGTAAGGGGAAGGAGGAGAAAGTTGAAGCCTTTAAGGCTGCTTTTTTGAGGACTGATCGTGATTTCCTAGAAAAGGGTGTTGTGAGTGGTGCTTGCTGTGTTACTGCTGTGATACAAGATCAGGAGATGATTGTATCAAATTTGGGAGATTGTAGAGCTGTCCTGTGTAGAGCAGGTGTTGCTGAGGCTCTTACTGATGATCACAAACCAGGAAGGGATGATGAAAAGGAAAGAATTGAGAGTCAGGGAGGTTATGTCGATAATCATCAAGGGGCTTGGCGAGTTCAGGGAATACTTGCGGTTTCCAGAAGCATTGGAGATGCACACTTAAAGAAGTGGGTGGTTGCTGAACCTGAGACGAGAGTACTAGAACTGGAACAAGATATGGAGTTTCTTGTCTTAGCTTCTGATGGACTTTGGGATGTGGTTAGCAATCAAGAAGCTGTTTATACCGTGTTGCATGTTCTAGCTCAGAGAAAGACGCCTAAAGAAAGTGAGGAGGAGAACTTGGTTCAGGGCTTTGTCAATATGAGCCCATCTTCGAAACTTCGTCGAGCTTCACTGGTCAAGTCTCCCAGATGTGCTAAATCCCAAAGTTACTACTACAACTCAGAGAATGAATCACCCTCACTCAACCGTGAAATAGGGAGTTCACCTTCAAAGTCACCGATAACACCGTGGAAGTCTCTTTGGGCCAAAGCGGCTTGCAAAGAGCTTGCGAACCTGGCTGCAAAAAGAGGCAGTATGGATGATATCACAGTGGTGATAATTGATCTCAACCATTACAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

445

Amino Acids

49.35

Weight (kDa)

8.43

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 84 - 309 2.5e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67820 AT1G67820
fragaria_vesca FvH4_4g25730
malus_domestica MD13G1099500.v1.1
prunus_persica Prupe.1G246400_v2.0.a1
pyrus_communis pycom13g08690
rosa_chinensis RchiOBHm_Chr4g0433171
rosa_laevigata RLG00000006777
rosa_multiflora Rmu_co8297519.1_g000001 Rmu_sc0000957.1_g000012 Rmu_ssc0000295.1_g000013
rosa_roxburghii Rroxscaffold_5G00374660
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320200 Rh4BG328400 Rh4CG343800 Rh4DG323800
rosa_wichuraiana Rw0G008700 Rw4G027800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 707
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 256, 707
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 2 cut(s) 842, 1244
AcsI RAATTY 3 cut(s) 494, 508, 682
AcuI CTGAAG 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 2 cut(s) 61, 909
AfiI CCNNNNNNNGG 1 cut(s) 616
AgsI TTSAA 3 cut(s) 186, 569, 1203
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 2 cut(s) 751, 1266
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AleI CACNNNNGTG 1 cut(s) 1304
AluBI AGCT 8 cut(s) 345, 488, 701, 947, 986, 1013, 1100, 1258
AluI AGCT 8 cut(s) 345, 488, 701, 947, 986, 1013, 1100, 1258
Alw26I GTCTC 8 cut(s) 121, 130, 229, 238, 376, 893, 1119, 1233
AoxI GGCC 1 cut(s) 1236
ApeKI GCWGC 3 cut(s) 488, 581, 1271
ApoI RAATTY 3 cut(s) 494, 508, 682
AspS9I GGNCC 1 cut(s) 1236
AsuHPI GGTGA 7 cut(s) 69, 224, 317, 1158, 1188, 1200, 1318
AsuII TTCGAA 2 cut(s) 292, 1085
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 1079
BbvCI CCTCAGC 1 cut(s) 726
BbvI GCAGC 3 cut(s) 475, 568, 1258
BccI CCATC 4 cut(s) 461, 529, 947, 1087
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 2 cut(s) 753, 1268
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 8 cut(s) 121, 130, 229, 238, 376, 893, 1119, 1233
BfaI CTAG 4 cut(s) 57, 611, 911, 1010
BfmI CTRYAG 1 cut(s) 489
BfuAI ACCTGC 2 cut(s) 256, 707
BglI GCCNNNNNGGC 2 cut(s) 578, 1244
BisI GCNGC 4 cut(s) 489, 582, 1245, 1272
BlsI GCNGC 4 cut(s) 490, 583, 1246, 1273
BmcAI AGTACT 1 cut(s) 909
Bme1390I CCNGG 2 cut(s) 753, 1268
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 1236
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 2 cut(s) 753, 1268
BmsI GCATC 1 cut(s) 853
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 2 cut(s) 292, 1085
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 4 cut(s) 121, 130, 229, 238
BsaJI CCNNGG 1 cut(s) 1220
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse1I ACTGG 2 cut(s) 921, 1110
Bse3DI GCAATG 2 cut(s) 69, 361
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 2 cut(s) 753, 1268
BseDI CCNNGG 1 cut(s) 1220
BseGI GGATG 3 cut(s) 766, 970, 1297
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 2 cut(s) 69, 361
BseMII CTCAG 6 cut(s) 33, 495, 717, 888, 1028, 1167
BseNI ACTGG 2 cut(s) 921, 1110
BseRI GAGGAG 3 cut(s) 572, 1055, 1058
BseXI GCAGC 3 cut(s) 475, 568, 1258
BshFI GGCC 1 cut(s) 1238
BshNI GGYRCC 1 cut(s) 335
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 8 cut(s) 121, 130, 229, 238, 376, 893, 1119, 1233
BsmBI CGTCTC 2 cut(s) 376, 893
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 1 cut(s) 1238
Bso31I GGTCTC 4 cut(s) 121, 130, 229, 238
Bsp119I TTCGAA 2 cut(s) 292, 1085
Bsp1286I GDGCHC 1 cut(s) 1079
Bsp143I GATC 4 cut(s) 598, 661, 742, 1315
BspACI CCGC 2 cut(s) 842, 1244
BspANI GGCC 1 cut(s) 1238
BspCNI CTCAG 6 cut(s) 32, 496, 718, 889, 1027, 1166
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 2 cut(s) 256, 707
BspT104I TTCGAA 2 cut(s) 292, 1085
BspT107I GGYRCC 1 cut(s) 335
BspTNI GGTCTC 4 cut(s) 121, 130, 229, 238
BsrDI GCAATG 2 cut(s) 69, 361
BsrI ACTGG 2 cut(s) 921, 1110
BssECI CCNNGG 1 cut(s) 1220
BssMI GATC 4 cut(s) 598, 661, 742, 1315
BssNI GRCGYC 1 cut(s) 1025
Bst2UI CCWGG 2 cut(s) 753, 1268
Bst4CI ACNGT 6 cut(s) 340, 383, 997, 1181, 1221, 1303
BstACI GRCGYC 1 cut(s) 1025
BstAPI GCANNNNNTGC 1 cut(s) 722
BstBI TTCGAA 2 cut(s) 292, 1085
BstC8I GCNNGC 3 cut(s) 637, 1249, 1260
BstDEI CTNAG 8 cut(s) 19, 65, 504, 726, 897, 943, 1014, 1153
BstDSI CCRYGG 1 cut(s) 1220
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 3 cut(s) 766, 970, 1297
BstKTI GATC 4 cut(s) 601, 664, 745, 1318
BstMAI GTCTC 8 cut(s) 121, 130, 229, 238, 376, 893, 1119, 1233
BstMBI GATC 4 cut(s) 598, 661, 742, 1315
BstMWI GCNNNNNNNGC 7 cut(s) 342, 351, 488, 578, 722, 1244, 1268
BstNI CCWGG 2 cut(s) 753, 1268
BstNSI RCATGY 1 cut(s) 1007
BstSCI CCNGG 2 cut(s) 751, 1266
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 475, 568, 1258
BsuRI GGCC 1 cut(s) 1238
BtgI CCRYGG 1 cut(s) 1220
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 3 cut(s) 766, 970, 1297
BtsIMutI CAGTG 2 cut(s) 1103, 1308
BveI ACCTGC 2 cut(s) 256, 707
Cac8I GCNNGC 3 cut(s) 637, 1249, 1260
Cfr13I GGNCC 1 cut(s) 1236
CseI GACGC 3 cut(s) 63, 224, 1033
Csp6I GTAC 2 cut(s) 60, 908
CviAII CATG 3 cut(s) 52, 473, 1004
CviQI GTAC 2 cut(s) 60, 908
DdeI CTNAG 8 cut(s) 19, 65, 504, 726, 897, 943, 1014, 1153
DpnI GATC 4 cut(s) 600, 663, 744, 1317
DpnII GATC 4 cut(s) 598, 661, 742, 1315
DraI TTTAAA 2 cut(s) 126, 234
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 1079
Eco31I GGTCTC 4 cut(s) 121, 130, 229, 238
Eco32I GATATC 1 cut(s) 1297
Eco57I CTGAAG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 751, 1266
EcoRV GATATC 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 1079
Esp3I CGTCTC 2 cut(s) 376, 893
FaeI CATG 3 cut(s) 55, 476, 1007
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 51, 472, 1003
FbaI TGATCA 1 cut(s) 742
Fnu4HI GCNGC 4 cut(s) 489, 582, 1245, 1272
FokI GGATG 3 cut(s) 773, 977, 1304
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 4 cut(s) 489, 582, 1245, 1272
FspBI CTAG 4 cut(s) 57, 611, 911, 1010
GluI GCNGC 4 cut(s) 489, 582, 1245, 1272
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 1 cut(s) 1238
HgaI GACGC 3 cut(s) 63, 224, 1033
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 3 cut(s) 55, 476, 1007
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 3 cut(s) 289, 784, 1163
HphI GGTGA 7 cut(s) 69, 224, 317, 1158, 1188, 1200, 1318
Hpy166II GTNNAC 2 cut(s) 62, 1196
Hpy188I TCNGA 5 cut(s) 22, 206, 952, 1017, 1156
Hpy188III TCNNGA 5 cut(s) 375, 602, 665, 849, 980
Hpy8I GTNNAC 2 cut(s) 62, 1196
Hpy99I CGWCG 1 cut(s) 1098
HpyAV CCTTC 3 cut(s) 547, 750, 1209
HpyCH4III ACNGT 6 cut(s) 340, 383, 997, 1181, 1221, 1303
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 6 cut(s) 491, 514, 866, 1003, 1251, 1274
HpyF10VI GCNNNNNNNGC 7 cut(s) 342, 351, 488, 578, 722, 1244, 1268
HpyF3I CTNAG 8 cut(s) 19, 65, 504, 726, 897, 943, 1014, 1153
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 3 cut(s) 55, 476, 1007
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 4 cut(s) 598, 661, 742, 1315
Lsp1109I GCAGC 3 cut(s) 475, 568, 1258
LweI GCATC 1 cut(s) 853
MaeI CTAG 4 cut(s) 57, 611, 911, 1010
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 7 cut(s) 75, 106, 212, 434, 643, 1139, 1206
MalI GATC 4 cut(s) 600, 663, 744, 1317
MboI GATC 4 cut(s) 598, 661, 742, 1315
MboII GAAGA 5 cut(s) 35, 36, 400, 413, 1074
MhlI GDGCHC 1 cut(s) 1079
MluCI AATT 7 cut(s) 414, 494, 508, 541, 682, 777, 1311
MlyI GAGTC 1 cut(s) 793
MseI TTAA 5 cut(s) 125, 149, 233, 576, 872
MslI CAYNNNNRTG 3 cut(s) 477, 519, 1304
MspR9I CCNGG 2 cut(s) 753, 1268
MvaI CCWGG 2 cut(s) 753, 1268
MwoI GCNNNNNNNGC 7 cut(s) 342, 351, 488, 578, 722, 1244, 1268
NdeII GATC 4 cut(s) 598, 661, 742, 1315
NlaIII CATG 3 cut(s) 55, 476, 1007
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 4 cut(s) 75, 106, 212, 1206
NspI RCATGY 1 cut(s) 1007
NspV TTCGAA 2 cut(s) 292, 1085
OliI CACNNNNGTG 1 cut(s) 1304
PaqCI CACCTGC 1 cut(s) 707
PfeI GAWTC 2 cut(s) 289, 1163
PkrI GCNGC 4 cut(s) 490, 583, 1246, 1273
PleI GAGTC 1 cut(s) 792
PpsI GAGTC 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 751, 1266
PspGI CCWGG 2 cut(s) 751, 1266
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 1236
PstI CTGCAG 1 cut(s) 493
RsaI GTAC 2 cut(s) 61, 909
RsaNI GTAC 2 cut(s) 60, 908
RseI CAYNNNNRTG 3 cut(s) 477, 519, 1304
SaqAI TTAA 5 cut(s) 125, 149, 233, 576, 872
SatI GCNGC 4 cut(s) 489, 582, 1245, 1272
Sau3AI GATC 4 cut(s) 598, 661, 742, 1315
Sau96I GGNCC 1 cut(s) 1236
ScaI AGTACT 1 cut(s) 909
SchI GAGTC 1 cut(s) 793
ScrFI CCNGG 2 cut(s) 753, 1268
SduI GDGCHC 1 cut(s) 1079
SfaNI GCATC 1 cut(s) 853
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 2 cut(s) 292, 1085
SmiMI CAYNNNNRTG 3 cut(s) 477, 519, 1304
Sse9I AATT 7 cut(s) 414, 494, 508, 541, 682, 777, 1311
SsiI CCGC 2 cut(s) 842, 1244
SspMI CTAG 4 cut(s) 57, 611, 911, 1010
StyD4I CCNGG 2 cut(s) 751, 1266
TaaI ACNGT 6 cut(s) 340, 383, 997, 1181, 1221, 1303
TaiI ACGT 1 cut(s) 38
TaqI TCGA 4 cut(s) 292, 801, 1085, 1096
TaqII GACCGA 1 cut(s) 94
TasI AATT 7 cut(s) 414, 494, 508, 541, 682, 777, 1311
TatI WGTACW 2 cut(s) 59, 907
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 2 cut(s) 289, 1163
Tru1I TTAA 5 cut(s) 125, 149, 233, 576, 872
Tru9I TTAA 5 cut(s) 125, 149, 233, 576, 872
TscAI CASTG 2 cut(s) 1110, 1308
TseFI GTSAC 4 cut(s) 75, 106, 212, 1206
TseI GCWGC 3 cut(s) 488, 581, 1271
Tsp45I GTSAC 4 cut(s) 75, 106, 212, 1206
TspDTI ATGAA 3 cut(s) 40, 780, 1176
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 2 cut(s) 1110, 1308
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 3 cut(s) 494, 508, 682
XceI RCATGY 1 cut(s) 1007
XspI CTAG 4 cut(s) 57, 611, 911, 1010
ZrmI AGTACT 1 cut(s) 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.