Rh4AG320200

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012422
Physical Location & Seq
Forward (+)
63293042 .. 63296025
2984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG320200.1

Sequence Viewer

Length: 1629 bp
ATGACGACGACCACAGAAAACGCCGCCGTTTCACATTCCGACGCTTCTTCAATCGCCGCTGCGCTGGATCCAATTACCGCATTTTCTTCTCTGAAGCGGAAGCGACCGCCGAAGATTGAGATCCCAAACGTCCTGCAAGAAATTAAAACCGAGAAGCAGCGTGACTGGGGGCCGCGAAACGGCGTCGTTTGCTTCAGTGGGATCGGAGTTGGGGTCTCCTCCGCCAAAGGCAAGAAGAAGTTTATGGAAGACGCGCATAAGATTGTTTCCTGCTTACAAGGCAACTCAAAGAGAGGTTTCTTTGGGGTTTATGATGGGCATGGTGGTAGTAAGGCTGCAGAGTTTGCTGCTGAGAATTTGCACACTAACGTTCTTGAAATGATGGAAGCTTGTGCAGAAAAAGAAGAGGCCTTTAGAGCTGGGTATCTGAAAACAGACCACGAGTTCTTGAAACAGGGTGTAGGGAGTGGTACGTGCTGTGTAACTGCCTTAGTCGATGAGCAGGAGGTGGTTATTTCGAACGTGGGAGATTGTAGGGCTGTTCTCTCCAGAGGTGGAGTGGCCGAAGCACTTACAAGAGATCATACTGCGGCACAGGAGGGCGAACGCCAAAGAATACAGGATAAGGGAGGATATGTGGAGTTCCACAGAGGAGCATGGAGGGTCCATGGGGTTCTTTCTGTTTCAAGAAGCATAGGAGATGCTCATCTGAAGAGCTGGGTGATGGGTGAGCCTGAAACAAATGTCTTACAGCTGACTCCAGATATGGATTTTGTTGTCTTAGCCTCGGATGGACTGTGGGAAAAGGTTGGCAACCAAGAAGCAATTGACATGGTGACACGAACATGTTCAGTTCAGAAGAAAATGGCACCCCCAGGAAACGGATTGAAAGATAATGATGAAGACCATCTGTGTGTCAGTGTGAGCCCTTCATCAAAGTTGCAAAGGACCTTGTTGGTTAAGGAGCCAAAGGTGACCCAATCCCCAGGAGAACTCCAGAAAGATAATAATGATGAGAGTGGCTGTGTTAGTGCAAGCCCTTCATCAAAGTTGCAAAGGATTTCATTGGTTAAGCAACCAAAAGGGACTCAATCCCCTGGAGAACTACTGATAGATAATAATGAGGACTATGGCTATGTCAATGTGAGCCCTTCATCGAAGTTGCGAAGGATTTCCTTGGTTAAACAGCCAAAAGGGATGCAATCTCCAGGCTATAAGAAGACACTCTTTGGCTCTGAAGATGCTGATAATGACTTTTCAAGTGAAAATGAAAGCCCCCCATCAAAGTCAAGAAGACTATCAATGTTAAAACGAGTAAACATGAAGACTGAATCTCCCATAAAAGAGAAGTGTGGATGTAAAATCAGACCTACCTGTGGTGAGAACGAATTTGCAAGTGAAAACGAAAGCCCTCCATCAAAGTCAAGGAGAATATCATTGGTTAAGCGAGTAAACATGAAGAGTGAATCTCCCATAAAAGAGAACAGTGGATATAAAATGAGACCTGCCTCTGGTGGACTGGTGACTGCTTGCAAGGAGCTTGTGAACCTTGCTTTGAGTAGGGGTAGCTTGGATGACATCACCGTAATGATAATTGATCTCAATCATTTTAGATGCAACAGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

542

Amino Acids

59.01

Weight (kDa)

8.4

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 69 - 286 1.4e-56 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67820 AT1G67820
fragaria_vesca FvH4_4g25730
malus_domestica MD13G1099500.v1.1
prunus_persica Prupe.1G246400_v2.0.a1
pyrus_communis pycom13g08690
rosa_chinensis RchiOBHm_Chr4g0433171
rosa_laevigata RLG00000006777
rosa_multiflora Rmu_co8297519.1_g000001 Rmu_sc0000957.1_g000012 Rmu_ssc0000295.1_g000013
rosa_roxburghii Rroxscaffold_5G00374660
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320200 Rh4BG328400 Rh4CG343800 Rh4DG323800
rosa_wichuraiana Rw0G008700 Rw4G027800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1513
AccB1I GGYRCC 1 cut(s) 868
AccII CGCG 2 cut(s) 175, 254
AciI CCGC 8 cut(s) 24, 57, 78, 97, 107, 173, 222, 590
AclI AACGTT 1 cut(s) 369
AclWI GGATC 4 cut(s) 62, 75, 115, 209
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 2 cut(s) 355, 1388
AcuI CTGAAG 4 cut(s) 113, 178, 731, 1257
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 4 cut(s) 179, 881, 1376, 1511
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 6 cut(s) 51, 377, 451, 687, 889, 1260
AjnI CCWGG 4 cut(s) 874, 985, 1096, 1207
AluBI AGCT 6 cut(s) 389, 419, 717, 754, 1540, 1569
AluI AGCT 6 cut(s) 389, 419, 717, 754, 1540, 1569
Alw26I GTCTC 2 cut(s) 220, 1495
AlwI GGATC 4 cut(s) 62, 75, 115, 209
AoxI GGCC 3 cut(s) 170, 408, 561
ApeKI GCWGC 4 cut(s) 59, 157, 335, 347
ApoI RAATTY 2 cut(s) 355, 1388
Asp700I GAANNNNTTC 2 cut(s) 847, 1171
AspLEI GCGC 2 cut(s) 64, 256
AspS9I GGNCC 3 cut(s) 170, 664, 948
AsuHPI GGTGA 7 cut(s) 733, 740, 847, 985, 1391, 1534, 1573
AsuII TTCGAA 1 cut(s) 518
AvaII GGWCC 2 cut(s) 664, 948
BamHI GGATCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 868
BanII GRGCYC 2 cut(s) 929, 1151
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 5 cut(s) 255, 909, 1226, 1300, 1331
BbvI GCAGC 4 cut(s) 46, 169, 322, 334
BccI CCATC 7 cut(s) 308, 376, 718, 785, 915, 1288, 1423
BceAI ACGGC 2 cut(s) 11, 196
BciT130I CCWGG 4 cut(s) 876, 987, 1098, 1209
BcoDI GTCTC 2 cut(s) 220, 1495
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 1513
BisI GCNGC 8 cut(s) 24, 57, 60, 158, 173, 336, 348, 591
BlsI GCNGC 8 cut(s) 25, 58, 61, 159, 174, 337, 349, 592
Bme1390I CCNGG 4 cut(s) 876, 987, 1098, 1209
Bme18I GGWCC 2 cut(s) 664, 948
BmgT120I GGNCC 3 cut(s) 170, 664, 948
BmiI GGNNCC 5 cut(s) 69, 171, 665, 870, 966
BmrFI CCNGG 4 cut(s) 876, 987, 1098, 1209
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 4 cut(s) 691, 1188, 1231, 1604
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 5 cut(s) 255, 909, 1226, 1300, 1331
BplI GAGNNNNNCTC 2 cut(s) 1453, 1485
BpmI CTGGAG 5 cut(s) 532, 744, 980, 1119, 1191
Bpu14I TTCGAA 1 cut(s) 518
BsaAI YACGTR 1 cut(s) 474
BsaBI GATNNNNATC 3 cut(s) 119, 705, 1602
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 220, 1495
BsaJI CCNNGG 6 cut(s) 667, 786, 874, 985, 1096, 1176
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 621, 651, 1318, 1348
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 881, 1376, 1511
Bse1I ACTGG 2 cut(s) 170, 1524
Bse8I GATNNNNATC 3 cut(s) 119, 705, 1602
BseBI CCWGG 4 cut(s) 876, 987, 1098, 1209
BseDI CCNNGG 6 cut(s) 667, 786, 874, 985, 1096, 1176
BseGI GGATG 4 cut(s) 796, 1203, 1361, 1579
BseJI GATNNNNATC 3 cut(s) 119, 705, 1602
BseLI CCNNNNNNNGG 4 cut(s) 179, 881, 1376, 1511
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 170, 1524
BseRI GAGGAG 2 cut(s) 208, 666
BseXI GCAGC 4 cut(s) 46, 169, 322, 334
BseYI CCCAGC 2 cut(s) 419, 717
BsgI GTGCAG 1 cut(s) 414
Bsh1236I CGCG 2 cut(s) 175, 254
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 3 cut(s) 172, 410, 563
BshNI GGYRCC 1 cut(s) 868
BsiEI CGRYCG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 1099
BslI CCNNNNNNNGG 4 cut(s) 179, 881, 1376, 1511
BsmAI GTCTC 2 cut(s) 220, 1495
BsmFI GGGAC 1 cut(s) 1099
BsnI GGCC 3 cut(s) 172, 410, 563
Bso31I GGTCTC 2 cut(s) 220, 1495
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 929, 1151
Bsp143I GATC 5 cut(s) 67, 120, 201, 580, 1597
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 8 cut(s) 24, 57, 78, 97, 107, 173, 222, 590
BspANI GGCC 3 cut(s) 172, 410, 563
BspCNI CTCAG 1 cut(s) 343
BspFNI CGCG 2 cut(s) 175, 254
BspLI GGNNCC 5 cut(s) 69, 171, 665, 870, 966
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 1513
BspPI GGATC 4 cut(s) 62, 75, 115, 209
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 868
BspTNI GGTCTC 2 cut(s) 220, 1495
BsrI ACTGG 2 cut(s) 170, 1524
BssECI CCNNGG 6 cut(s) 667, 786, 874, 985, 1096, 1176
BssMI GATC 5 cut(s) 67, 120, 201, 580, 1597
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 2 cut(s) 667, 1176
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 4 cut(s) 876, 987, 1098, 1209
Bst4CI ACNGT 4 cut(s) 798, 1487, 1585, 1622
Bst6I CTCTTC 3 cut(s) 399, 707, 1454
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 1036, 1531
BstDEI CTNAG 3 cut(s) 351, 490, 781
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 973
BstF5I GGATG 4 cut(s) 796, 1203, 1361, 1579
BstFNI CGCG 2 cut(s) 175, 254
BstHHI GCGC 2 cut(s) 64, 256
BstKTI GATC 5 cut(s) 70, 123, 204, 583, 1600
BstMAI GTCTC 2 cut(s) 220, 1495
BstMBI GATC 5 cut(s) 67, 120, 201, 580, 1597
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 4 cut(s) 189, 279, 344, 416
BstNI CCWGG 4 cut(s) 876, 987, 1098, 1209
BstNSI RCATGY 1 cut(s) 849
BstPI GGTNACC 1 cut(s) 973
BstSCI CCNGG 4 cut(s) 874, 985, 1096, 1207
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 175, 254
BstV1I GCAGC 4 cut(s) 46, 169, 322, 334
BstV2I GAAGAC 5 cut(s) 255, 909, 1226, 1300, 1331
BstX2I RGATCY 2 cut(s) 67, 120
BstYI RGATCY 2 cut(s) 67, 120
BsuRI GGCC 3 cut(s) 172, 410, 563
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 4 cut(s) 796, 1203, 1361, 1579
BtsIMutI CAGTG 4 cut(s) 202, 925, 1492, 1627
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 2 cut(s) 1036, 1531
CfoI GCGC 2 cut(s) 64, 256
Cfr13I GGNCC 3 cut(s) 170, 664, 948
CseI GACGC 3 cut(s) 50, 172, 260
Csp6I GTAC 1 cut(s) 471
CviAII CATG 7 cut(s) 320, 657, 668, 832, 846, 1321, 1456
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 3 cut(s) 351, 490, 781
DpnI GATC 5 cut(s) 69, 122, 203, 582, 1599
DpnII GATC 5 cut(s) 67, 120, 201, 580, 1597
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 3 cut(s) 399, 707, 1454
EarI CTCTTC 3 cut(s) 399, 707, 1454
EciI GGCGGA 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 667, 1176
Eco147I AGGCCT 1 cut(s) 410
Eco24I GRGCYC 2 cut(s) 929, 1151
Eco31I GGTCTC 2 cut(s) 220, 1495
Eco47I GGWCC 2 cut(s) 664, 948
Eco57I CTGAAG 4 cut(s) 113, 178, 731, 1257
Eco91I GGTNACC 1 cut(s) 973
EcoO109I RGGNCCY 1 cut(s) 948
EcoO65I GGTNACC 1 cut(s) 973
EcoRII CCWGG 4 cut(s) 874, 985, 1096, 1207
EcoT14I CCWWGG 2 cut(s) 667, 1176
EcoT38I GRGCYC 2 cut(s) 929, 1151
ErhI CCWWGG 2 cut(s) 667, 1176
FaeI CATG 7 cut(s) 323, 660, 671, 835, 849, 1324, 1459
FalI AAGNNNNNCTT 2 cut(s) 1211, 1243
FaqI GGGAC 1 cut(s) 1099
FatI CATG 7 cut(s) 319, 656, 667, 831, 845, 1320, 1455
Fnu4HI GCNGC 8 cut(s) 24, 57, 60, 158, 173, 336, 348, 591
FokI GGATG 4 cut(s) 803, 1210, 1368, 1586
FriOI GRGCYC 2 cut(s) 929, 1151
Fsp4HI GCNGC 8 cut(s) 24, 57, 60, 158, 173, 336, 348, 591
GlaI GCGC 2 cut(s) 63, 255
GluI GCNGC 8 cut(s) 24, 57, 60, 158, 173, 336, 348, 591
GsaI CCCAGC 2 cut(s) 423, 721
GsuI CTGGAG 5 cut(s) 532, 744, 980, 1119, 1191
HaeIII GGCC 3 cut(s) 172, 410, 563
HgaI GACGC 3 cut(s) 50, 172, 260
HhaI GCGC 2 cut(s) 64, 256
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 7 cut(s) 323, 660, 671, 835, 849, 1324, 1459
Hin6I GCGC 2 cut(s) 62, 254
HinP1I GCGC 2 cut(s) 62, 254
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 4 cut(s) 757, 1087, 1331, 1466
HphI GGTGA 7 cut(s) 733, 740, 847, 985, 1391, 1534, 1573
Hpy166II GTNNAC 4 cut(s) 1318, 1453, 1517, 1546
Hpy188I TCNGA 9 cut(s) 40, 93, 206, 429, 711, 790, 858, 1237, 1367
Hpy188III TCNNGA 7 cut(s) 374, 448, 549, 687, 761, 997, 1290
Hpy8I GTNNAC 4 cut(s) 1318, 1453, 1517, 1546
Hpy99I CGWCG 3 cut(s) 10, 44, 188
HpyAV CCTTC 4 cut(s) 939, 1050, 1161, 1161
HpyCH4III ACNGT 4 cut(s) 798, 1487, 1585, 1622
HpyCH4IV ACGT 4 cut(s) 129, 369, 473, 522
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 279, 344, 416
HpyF3I CTNAG 3 cut(s) 351, 490, 781
HpySE526I ACGT 4 cut(s) 129, 369, 473, 522
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 7 cut(s) 323, 660, 671, 835, 849, 1324, 1459
HspAI GCGC 2 cut(s) 62, 254
Kzo9I GATC 5 cut(s) 67, 120, 201, 580, 1597
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 3 cut(s) 653, 964, 1537
Lsp1109I GCAGC 4 cut(s) 46, 169, 322, 334
LweI GCATC 4 cut(s) 691, 1188, 1231, 1604
MaeII ACGT 4 cut(s) 129, 369, 473, 522
MaeIII GTNAC 5 cut(s) 161, 481, 835, 973, 1522
MalI GATC 5 cut(s) 69, 122, 203, 582, 1599
MboI GATC 5 cut(s) 67, 120, 201, 580, 1597
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 2 cut(s) 67, 120
MhlI GDGCHC 2 cut(s) 929, 1151
MluCI AATT 6 cut(s) 72, 141, 355, 825, 1388, 1593
MlyI GAGTC 2 cut(s) 751, 1081
MmeI TCCRAC 1 cut(s) 63
MroXI GAANNNNTTC 2 cut(s) 847, 1171
MseI TTAA 6 cut(s) 144, 960, 1071, 1182, 1307, 1443
MslI CAYNNNNRTG 3 cut(s) 844, 912, 1586
MspA1I CMGCKG 2 cut(s) 59, 754
MspR9I CCNGG 4 cut(s) 876, 987, 1098, 1209
MunI CAATTG 1 cut(s) 825
MvaI CCWGG 4 cut(s) 876, 987, 1098, 1209
MvnI CGCG 2 cut(s) 175, 254
MwoI GCNNNNNNNGC 4 cut(s) 189, 279, 344, 416
NcoI CCATGG 1 cut(s) 667
NdeII GATC 5 cut(s) 67, 120, 201, 580, 1597
NlaIII CATG 7 cut(s) 323, 660, 671, 835, 849, 1324, 1459
NlaIV GGNNCC 5 cut(s) 69, 171, 665, 870, 966
NmuCI GTSAC 4 cut(s) 161, 835, 973, 1522
NspI RCATGY 1 cut(s) 849
NspV TTCGAA 1 cut(s) 518
PceI AGGCCT 1 cut(s) 410
PciI ACATGT 1 cut(s) 845
PciSI GCTCTTC 1 cut(s) 707
PdmI GAANNNNTTC 2 cut(s) 847, 1171
PfeI GAWTC 2 cut(s) 1331, 1466
PkrI GCNGC 8 cut(s) 25, 58, 61, 159, 174, 337, 349, 592
PleI GAGTC 2 cut(s) 751, 1081
PpsI GAGTC 2 cut(s) 751, 1081
Ppu21I YACGTR 1 cut(s) 474
PpuMI RGGWCCY 1 cut(s) 948
PscI ACATGT 1 cut(s) 845
Psp1406I AACGTT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 948
Psp6I CCWGG 4 cut(s) 874, 985, 1096, 1207
PspEI GGTNACC 1 cut(s) 973
PspFI CCCAGC 2 cut(s) 419, 717
PspGI CCWGG 4 cut(s) 874, 985, 1096, 1207
PspN4I GGNNCC 5 cut(s) 69, 171, 665, 870, 966
PspPI GGNCC 3 cut(s) 170, 664, 948
PspPPI RGGWCCY 1 cut(s) 948
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 2 cut(s) 67, 120
PvuII CAGCTG 1 cut(s) 754
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 3 cut(s) 844, 912, 1586
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 6 cut(s) 144, 960, 1071, 1182, 1307, 1443
SatI GCNGC 8 cut(s) 24, 57, 60, 158, 173, 336, 348, 591
Sau3AI GATC 5 cut(s) 67, 120, 201, 580, 1597
Sau96I GGNCC 3 cut(s) 170, 664, 948
SchI GAGTC 2 cut(s) 751, 1081
ScrFI CCNGG 4 cut(s) 876, 987, 1098, 1209
SduI GDGCHC 2 cut(s) 929, 1151
SfaNI GCATC 4 cut(s) 691, 1188, 1231, 1604
SfcI CTRYAG 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 518
SinI GGWCC 2 cut(s) 664, 948
SmiMI CAYNNNNRTG 3 cut(s) 844, 912, 1586
Sse9I AATT 6 cut(s) 72, 141, 355, 825, 1388, 1593
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 8 cut(s) 24, 57, 78, 97, 107, 173, 222, 590
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 4 cut(s) 874, 985, 1096, 1207
StyI CCWWGG 2 cut(s) 667, 1176
TaaI ACNGT 4 cut(s) 798, 1487, 1585, 1622
TaiI ACGT 4 cut(s) 132, 372, 476, 525
TaqI TCGA 3 cut(s) 495, 518, 1157
TasI AATT 6 cut(s) 72, 141, 355, 825, 1388, 1593
TauI GCSGC 4 cut(s) 26, 59, 175, 593
TfiI GAWTC 2 cut(s) 1331, 1466
Tru1I TTAA 6 cut(s) 144, 960, 1071, 1182, 1307, 1443
Tru9I TTAA 6 cut(s) 144, 960, 1071, 1182, 1307, 1443
TscAI CASTG 4 cut(s) 202, 925, 1492, 1627
TseFI GTSAC 4 cut(s) 161, 835, 973, 1522
TseI GCWGC 4 cut(s) 59, 157, 335, 347
Tsp45I GTSAC 4 cut(s) 161, 835, 973, 1522
TspDTI ATGAA 8 cut(s) 915, 921, 1032, 1053, 1143, 1284, 1337, 1472
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 4 cut(s) 202, 925, 1492, 1627
VpaK11BI GGWCC 2 cut(s) 664, 948
XapI RAATTY 2 cut(s) 355, 1388
XceI RCATGY 1 cut(s) 849
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XmnI GAANNNNTTC 2 cut(s) 847, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.