MD13G1099500.v1.1

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012422
Physical Location & Seq
Reverse (-)
7024186 .. 7026648
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1099500.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGCGAGCTCAGAACTCCCCGTTTCGCATTCCGATTCGTTATGCGCTACCGCCGCCGTGCTCGATGCGGTTGCCGCTTCATCTTCTTCTCTGAAGCGGAAGAGACCCCCGATGATTGCAATCCCGAACGTCCTGCAAGAAATTAAAACCGAGAAGCTCCGGGATTTCACCCCTCAGAACGGCGCCGTCTGCTTCAACGGGATTGGAGTTGGGGTCTCCGCCTCCAAAGGCAAGAAGAAGTTTATGGAAGATACCCACAAGATTGTTTCTTGCTTACAGGGACAGAAGTCAAAGAAAGCGTTTTTTGGGGTGTATGACGGGCATGGTGGGAAGAGGGCCGCAGACTTTGTTGCTGAGAATTTGCACAATAATATTGTCGAAATGATGGAAATGCATACGGAAACTGAAGAGGCAGTTAAAGCTGGGTATCTGAAGACAGACCAGGAGTTCTTGGAACAGGGCTTAGGCAGCGGAACCTGCTGTGTCACAGCCTTAATCGAAGGGCAGGAGGTTGTTATTTCAAACTTGGGAGATTGCCGGGCAGTTCTTTGTAGAGGTGGAGTGGCGGAAGCTCTTACAAAGGATCATAAGGCAGAACAGGAGGACGAACGCAACAGAATAGAGCATGAGGGAGGTTATGTAGAGTTCCACCGAGGAGCATGGAGAGTTCACGGGGTTCTTTCTGTTTCTAGAAGCATTGGAGATGCTCATCTGAAAAACTGGGTGGTGGCCGAGCCTGAAACAAAGATCCTGCAGCTGACTCCAGATATGGAATTTCTTGTATTGGCTTCGGATGGACTATGGGGAGAGGTTGACAACCAAGAAGCCATCGATACTGTGAAACGATTATGTTCAATTCAGAAGAAATCGGCACCCTCAGGAGATCTTCTGAATGACGATGACTATGGCTGTGTCAGTGTGAGCCCTTCATCAAAGTTGCGAAGGATTTCGCTGGTTAAGCAAGTAAAAGGGATGCAATCCCCAGGCTTTAAGAAGGCGGTCAATAGCTGGCAAGGTAGTGAGAATGACCTTGCAAGTGAAAATGAAAGCCCTCCATCAAAGGCAAGAAGAATATCCTTAGTAAAGCGATTAAACACAAAGATGGAATCACCCAAAAAAGAGACTCAATCTCCAGATAGCTGGAAAGATAGCGAGAATGAATTTCCGAATGAAAATGAAAGGCCTCCATCAAAGTCGAGAAGAATATCAGTGGTAAAGCGAGTAAGCACGAAGATTCAACCTACCTCTTCGGGTGGACTGGTGGCTGCTTGTAAGGAGCTTGTGAGACTTGCCACGAGTAGGGGTAGTTTGGATGACATCACTGTGATGATAATTGATCTCAATCACTTCAGATGCAACAGTGGATTCGCCCAGCTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

461

Amino Acids

50.27

Weight (kDa)

7.15

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 69 - 285 3.5e-57 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67820 AT1G67820
fragaria_vesca FvH4_4g25730
malus_domestica MD13G1099500.v1.1
prunus_persica Prupe.1G246400_v2.0.a1
pyrus_communis pycom13g08690
rosa_chinensis RchiOBHm_Chr4g0433171
rosa_laevigata RLG00000006777
rosa_multiflora Rmu_co8297519.1_g000001 Rmu_sc0000957.1_g000012 Rmu_ssc0000295.1_g000013
rosa_roxburghii Rroxscaffold_5G00374660
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320200 Rh4BG328400 Rh4CG343800 Rh4DG323800
rosa_wichuraiana Rw0G008700 Rw4G027800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 485
AccB1I GGYRCC 2 cut(s) 182, 871
AclWI GGATC 2 cut(s) 591, 742
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 3 cut(s) 358, 773, 1160
AcuI CTGAAG 4 cut(s) 113, 426, 452, 1333
AcyI GRCGYC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 179, 1299
AgsI TTSAA 4 cut(s) 196, 522, 855, 1238
AjnI CCWGG 2 cut(s) 441, 982
AluBI AGCT 9 cut(s) 9, 157, 422, 572, 757, 1008, 1140, 1279, 1375
AluI AGCT 9 cut(s) 9, 157, 422, 572, 757, 1008, 1140, 1279, 1375
Alw21I GWGCWC 2 cut(s) 11, 63
Alw26I GTCTC 4 cut(s) 97, 220, 1115, 1279
AlwI GGATC 2 cut(s) 591, 742
AoxI GGCC 3 cut(s) 336, 729, 1181
ApeKI GCWGC 3 cut(s) 468, 754, 1265
ApoI RAATTY 3 cut(s) 358, 773, 1160
Asp700I GAANNNNTTC 1 cut(s) 946
AspLEI GCGC 2 cut(s) 47, 185
AspS9I GGNCC 1 cut(s) 336
AsuC2I CCSGG 2 cut(s) 161, 539
AsuHPI GGTGA 2 cut(s) 160, 1101
AxyI CCTNAGG 1 cut(s) 877
BanI GGYRCC 2 cut(s) 182, 871
BanII GRGCYC 2 cut(s) 11, 926
BauI CACGAG 1 cut(s) 1294
BbsI GAAGAC 1 cut(s) 440
Bbv12I GWGCWC 2 cut(s) 11, 63
BbvI GCAGC 3 cut(s) 480, 766, 1252
BccI CCATC 6 cut(s) 379, 788, 836, 1063, 1096, 1195
BceAI ACGGC 3 cut(s) 41, 170, 196
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 115, 149
BciT130I CCWGG 2 cut(s) 443, 984
BcnI CCSGG 2 cut(s) 161, 539
BcoDI GTCTC 4 cut(s) 97, 220, 1115, 1279
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 1 cut(s) 752
BfoI RGCGCY 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 485
BglII AGATCT 1 cut(s) 883
BisI GCNGC 6 cut(s) 54, 75, 339, 469, 755, 1266
BlsI GCNGC 6 cut(s) 55, 76, 340, 470, 756, 1267
Bme1390I CCNGG 4 cut(s) 161, 443, 539, 984
BmgT120I GGNCC 1 cut(s) 336
BmiI GGNNCC 3 cut(s) 184, 475, 873
BmrFI CCNGG 4 cut(s) 161, 443, 539, 984
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 4 cut(s) 55, 694, 963, 1343
BmuI ACTGGG 1 cut(s) 730
BoxI GACNNNNGTC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 440
BpmI CTGGAG 2 cut(s) 747, 1116
Bpu10I CCTNAGC 1 cut(s) 463
BpuMI CCSGG 2 cut(s) 161, 539
Bsa29I ATCGAT 1 cut(s) 831
BsaBI GATNNNNATC 3 cut(s) 119, 708, 1341
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 97, 220
BsaJI CCNNGG 2 cut(s) 652, 982
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 624, 654, 1114, 1144
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 1299
Bse1I ACTGG 2 cut(s) 725, 1263
Bse21I CCTNAGG 1 cut(s) 877
Bse8I GATNNNNATC 3 cut(s) 119, 708, 1341
BseBI CCWGG 2 cut(s) 443, 984
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 2 cut(s) 652, 982
BseGI GGATG 3 cut(s) 799, 978, 1318
BseJI GATNNNNATC 3 cut(s) 119, 708, 1341
BseLI CCNNNNNNNGG 2 cut(s) 179, 1299
BseMII CTCAG 4 cut(s) 24, 188, 345, 891
BseNI ACTGG 2 cut(s) 725, 1263
BseRI GAGGAG 1 cut(s) 669
BseXI GCAGC 3 cut(s) 480, 766, 1252
BseYI CCCAGC 2 cut(s) 422, 1371
BshFI GGCC 3 cut(s) 338, 731, 1183
BshNI GGYRCC 2 cut(s) 182, 871
BshVI ATCGAT 1 cut(s) 831
BsiHKAI GWGCWC 2 cut(s) 11, 63
BsiSI CCGG 2 cut(s) 160, 538
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 179, 1299
BsmAI GTCTC 4 cut(s) 97, 220, 1115, 1279
BsmFI GGGAC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 3 cut(s) 338, 731, 1183
Bso31I GGTCTC 2 cut(s) 97, 220
Bsp1286I GDGCHC 3 cut(s) 11, 63, 926
Bsp143I GATC 4 cut(s) 583, 747, 883, 1336
BspANI GGCC 3 cut(s) 338, 731, 1183
BspCNI CTCAG 4 cut(s) 23, 187, 346, 890
BspDI ATCGAT 1 cut(s) 831
BspLI GGNNCC 3 cut(s) 184, 475, 873
BspMAI CTGCAG 1 cut(s) 756
BspMI ACCTGC 1 cut(s) 485
BspPI GGATC 2 cut(s) 591, 742
BspT107I GGYRCC 2 cut(s) 182, 871
BspTNI GGTCTC 2 cut(s) 97, 220
BsrI ACTGG 2 cut(s) 725, 1263
BssECI CCNNGG 2 cut(s) 652, 982
BssMI GATC 4 cut(s) 583, 747, 883, 1336
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 1294
Bst2BI CACGAG 1 cut(s) 1294
Bst2UI CCWGG 2 cut(s) 443, 984
Bst4CI ACNGT 3 cut(s) 838, 1324, 1361
Bst6I CTCTTC 4 cut(s) 95, 326, 402, 1252
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 7, 1010
BstDEI CTNAG 6 cut(s) 10, 174, 354, 463, 877, 1078
BstF5I GGATG 3 cut(s) 799, 978, 1318
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 47, 185
BstKTI GATC 4 cut(s) 586, 750, 886, 1339
BstMAI GTCTC 4 cut(s) 97, 220, 1115, 1279
BstMBI GATC 4 cut(s) 583, 747, 883, 1336
BstMWI GCNNNNNNNGC 7 cut(s) 53, 74, 189, 419, 468, 477, 958
BstNI CCWGG 2 cut(s) 443, 984
BstPAI GACNNNNGTC 1 cut(s) 286
BstSCI CCNGG 4 cut(s) 159, 441, 537, 982
BstSFI CTRYAG 1 cut(s) 752
BstV1I GCAGC 3 cut(s) 480, 766, 1252
BstV2I GAAGAC 1 cut(s) 440
BstX2I RGATCY 2 cut(s) 747, 883
BstXI CCANNNNNNTGG 1 cut(s) 1140
BstYI RGATCY 2 cut(s) 747, 883
Bsu15I ATCGAT 1 cut(s) 831
Bsu36I CCTNAGG 1 cut(s) 877
BsuRI GGCC 3 cut(s) 338, 731, 1183
BsuTUI ATCGAT 1 cut(s) 831
BtsCI GGATG 3 cut(s) 799, 978, 1318
BtsIMutI CAGTG 4 cut(s) 922, 1215, 1320, 1366
BveI ACCTGC 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 7, 1010
CfoI GCGC 2 cut(s) 47, 185
Cfr13I GGNCC 1 cut(s) 336
ClaI ATCGAT 1 cut(s) 831
CviAII CATG 3 cut(s) 323, 626, 660
DdeI CTNAG 6 cut(s) 10, 174, 354, 463, 877, 1078
DinI GGCGCC 1 cut(s) 184
DpnI GATC 4 cut(s) 585, 749, 885, 1338
DpnII GATC 4 cut(s) 583, 747, 883, 1336
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 4 cut(s) 95, 326, 402, 1252
EarI CTCTTC 4 cut(s) 95, 326, 402, 1252
EciI GGCGGA 2 cut(s) 208, 581
Ecl136II GAGCTC 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 1183
Eco24I GRGCYC 2 cut(s) 11, 926
Eco31I GGTCTC 2 cut(s) 97, 220
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 4 cut(s) 113, 426, 452, 1333
Eco81I CCTNAGG 1 cut(s) 877
EcoICRI GAGCTC 1 cut(s) 9
EcoRII CCWGG 2 cut(s) 441, 982
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 2 cut(s) 11, 926
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
FaeI CATG 3 cut(s) 326, 629, 663
FaqI GGGAC 1 cut(s) 294
FatI CATG 3 cut(s) 322, 625, 659
Fnu4HI GCNGC 6 cut(s) 54, 75, 339, 469, 755, 1266
FokI GGATG 3 cut(s) 806, 985, 1325
FriOI GRGCYC 2 cut(s) 11, 926
Fsp4HI GCNGC 6 cut(s) 54, 75, 339, 469, 755, 1266
FspBI CTAG 1 cut(s) 690
GlaI GCGC 2 cut(s) 46, 184
GluI GCNGC 6 cut(s) 54, 75, 339, 469, 755, 1266
GsaI CCCAGC 2 cut(s) 426, 1375
GsuI CTGGAG 2 cut(s) 747, 1116
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 3 cut(s) 338, 731, 1183
HapII CCGG 2 cut(s) 160, 538
HhaI GCGC 2 cut(s) 47, 185
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 326, 629, 663
Hin6I GCGC 2 cut(s) 45, 183
HinP1I GCGC 2 cut(s) 45, 183
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HinfI GANTC 6 cut(s) 35, 760, 1106, 1123, 1234, 1365
HpaII CCGG 2 cut(s) 160, 538
HphI GGTGA 2 cut(s) 160, 1101
Hpy166II GTNNAC 3 cut(s) 670, 814, 1256
Hpy188III TCNNGA 6 cut(s) 124, 690, 764, 879, 1133, 1197
Hpy8I GTNNAC 3 cut(s) 670, 814, 1256
HpyAV CCTTC 4 cut(s) 494, 936, 936, 988
HpyCH4III ACNGT 3 cut(s) 838, 1324, 1361
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 8 cut(s) 119, 136, 364, 394, 754, 976, 1034, 1356
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 74, 189, 419, 468, 477, 958
HpyF3I CTNAG 6 cut(s) 10, 174, 354, 463, 877, 1078
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 326, 629, 663
HspAI GCGC 2 cut(s) 45, 183
KasI GGCGCC 1 cut(s) 182
Kzo9I GATC 4 cut(s) 583, 747, 883, 1336
LmnI GCTCC 3 cut(s) 162, 656, 1276
Lsp1109I GCAGC 3 cut(s) 480, 766, 1252
LweI GCATC 4 cut(s) 55, 694, 963, 1343
MaeI CTAG 1 cut(s) 690
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 484
MalI GATC 4 cut(s) 585, 749, 885, 1338
MboI GATC 4 cut(s) 583, 747, 883, 1336
MflI RGATCY 2 cut(s) 747, 883
MhlI GDGCHC 3 cut(s) 11, 63, 926
MluCI AATT 6 cut(s) 141, 358, 773, 855, 1160, 1332
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 2 cut(s) 754, 1117
Mph1103I ATGCAT 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 946
MseI TTAA 6 cut(s) 144, 417, 494, 957, 990, 1091
MslI CAYNNNNRTG 3 cut(s) 1100, 1322, 1325
MspA1I CMGCKG 2 cut(s) 471, 757
MspI CCGG 2 cut(s) 160, 538
MspR9I CCNGG 4 cut(s) 161, 443, 539, 984
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 2 cut(s) 443, 984
MwoI GCNNNNNNNGC 7 cut(s) 53, 74, 189, 419, 468, 477, 958
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 2 cut(s) 161, 539
NdeII GATC 4 cut(s) 583, 747, 883, 1336
NlaIII CATG 3 cut(s) 326, 629, 663
NlaIV GGNNCC 3 cut(s) 184, 475, 873
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 396
PceI AGGCCT 1 cut(s) 1183
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 946
PfeI GAWTC 4 cut(s) 35, 1106, 1234, 1365
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 6 cut(s) 55, 76, 340, 470, 756, 1267
PleI GAGTC 2 cut(s) 754, 1117
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 2 cut(s) 754, 1117
PshAI GACNNNNGTC 1 cut(s) 286
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 441, 982
PspFI CCCAGC 2 cut(s) 422, 1371
PspGI CCWGG 2 cut(s) 441, 982
PspN4I GGNNCC 3 cut(s) 184, 475, 873
PspPI GGNCC 1 cut(s) 336
PstI CTGCAG 1 cut(s) 756
PsuI RGATCY 2 cut(s) 747, 883
PvuII CAGCTG 1 cut(s) 757
RseI CAYNNNNRTG 3 cut(s) 1100, 1322, 1325
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 6 cut(s) 144, 417, 494, 957, 990, 1091
SatI GCNGC 6 cut(s) 54, 75, 339, 469, 755, 1266
Sau3AI GATC 4 cut(s) 583, 747, 883, 1336
Sau96I GGNCC 1 cut(s) 336
SchI GAGTC 2 cut(s) 754, 1117
ScrFI CCNGG 4 cut(s) 161, 443, 539, 984
SduI GDGCHC 3 cut(s) 11, 63, 926
SfaNI GCATC 4 cut(s) 55, 694, 963, 1343
SfcI CTRYAG 1 cut(s) 752
SfoI GGCGCC 1 cut(s) 184
SmiMI CAYNNNNRTG 3 cut(s) 1100, 1322, 1325
Sse9I AATT 6 cut(s) 141, 358, 773, 855, 1160, 1332
SseBI AGGCCT 1 cut(s) 1183
SspDI GGCGCC 1 cut(s) 182
SspI AATATT 1 cut(s) 373
SspMI CTAG 1 cut(s) 690
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 1183
StyD4I CCNGG 4 cut(s) 159, 441, 537, 982
TaaI ACNGT 3 cut(s) 838, 1324, 1361
TaiI ACGT 1 cut(s) 132
TaqI TCGA 5 cut(s) 63, 378, 498, 831, 1196
TasI AATT 6 cut(s) 141, 358, 773, 855, 1160, 1332
TauI GCSGC 3 cut(s) 56, 77, 341
TfiI GAWTC 4 cut(s) 35, 1106, 1234, 1365
Tru1I TTAA 6 cut(s) 144, 417, 494, 957, 990, 1091
Tru9I TTAA 6 cut(s) 144, 417, 494, 957, 990, 1091
TscAI CASTG 4 cut(s) 922, 1215, 1327, 1366
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 3 cut(s) 468, 754, 1265
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 6 cut(s) 69, 918, 1059, 1173, 1185, 1191
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 4 cut(s) 922, 1215, 1327, 1366
XapI RAATTY 3 cut(s) 358, 773, 1160
XbaI TCTAGA 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 946
XspI CTAG 1 cut(s) 690
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.