Prupe.1G246400_v2.0.a1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012422
Physical Location & Seq
Forward (+)
25957401 .. 25960514
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G246400.1

Sequence Viewer

Length: 1533 bp
ATGGCGAGCTCAGAACTGGCCGTTTCGCATTCTGAGTCTTCAGCGGTTACCGCCACAGTAGATCCCATTACCGCTTCTTCTTCTCTGAAGCGGAAGAGACCCCCCATGATTGAGATCCCAAACGTCTTGCAAGAAATTAAAACCGAAAAGCTCAGAGATTTCACGCCAGAGAACGATGCCGTCTGCTTCAGTGGGATTGGAGTTGGGGTCTCCGCCGCCAAAGGCAAGAAAAAGTTTATGGAAGATACCCACAAGATAGTTTCTTGCTTACAAGGCAACAACTCCAAGAAGGGTTTCTTTGGGGTGTATGATGGGCATGGTGGCAAGAAGGCTGCAGATTTTGTTGCTGACAATTTGCACAATAATATTCTTGAAATGATGGAAGTGCATACAGAAATAGAAGAGGCGGTTAAAGCTGGGTATCTGAAAACAGACCAGGAGTTCTTGAAACAGGGTTTAGGAAGTGGTACCTGCTGTGTCACAGCCTTAATTGAAGGGCAAGAGGTCGTTATTTCAAACTTGGGGGATTGCAGGGCTGTTCTTTGTAGAGGTGGAGTGGCGGAGGCTCTTACCTTGGATCATACGGCAGAACAGGAGGATGAACGCAAAAGAATAGAGAATGAGGGAGGATATGTAGAGTTCCACCGAGGAGCATGGAGAGTTCATGGAGTTCTTTCTGTTTCTAGAAGCATTGGAGATGCTCATCTGAAGGACTGGGTGCTGGCTGAGCCTGAAACAAAGATCCTACAGCTCACTCCAGATATGGAATTTCTTGTATTGGCTTCGGATGGACTGTGGGGAGAGGTTGGCAACCAAGAAGCCATTGATACGGTGACCCGATTATGTTCAGTTCAGAAAAAATTGGGACCCTCAGGAGATCTACTGAAGGATAATGAGGAGGACTATGGCTGTGTTAGTGTGAGCCCTTCATCAAAGTTACGAAGAATTTCGCTAGTTAAGAAACTTAAAGGCACACAATCTCCAGGCTATAAGAAGACAGTCAATAGCTGGAAAGAGAGTGAGAATGACTTCCCGAGTGAAAATGAGAGCCCTCCATCGAAGTCAAGAAGAATCTCTTTAGCAAAGCGAGCAAACATGAAGAATGAATCTCCCATAAAAGAGACTCAATCTCCAGGCTATAAGAAGACTGACAATAGCTGCAAAGATAGTGAGAATGACTTCGCGAGTGAAAATGAAAGCCCCGCATCGAAGTCAAGAAAAATATCCTTAGTAAAGCGAGTAAACATGAAGAACGAATCTCCCGTAAAAGATACTCAATCTCCAGGCTATAAGAAGACAGTTAATAGCTGGAAAGATAGTGAGAATGACTTTCCAAGTGAAAATGAAAGCCCTCCATCAAAGTCAAGAAGAATATCATTGGTAAAGCGAGTAAATATGAAGATTCAACCAGCCTCCGGCGGACTGGTGGCTGCTTGTAAGGAGCTTGTGAACCTTGCATTGAGTAGGGGTAGTTTGGACGACATCACAGTGATGATAATTGATCTAAATCATTTCAGATGCCAGCTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

511

Amino Acids

55.95

Weight (kDa)

6.82

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67820 AT1G67820
fragaria_vesca FvH4_4g25730
malus_domestica MD13G1099500.v1.1
prunus_persica Prupe.1G246400_v2.0.a1
pyrus_communis pycom13g08690
rosa_chinensis RchiOBHm_Chr4g0433171
rosa_laevigata RLG00000006777
rosa_multiflora Rmu_co8297519.1_g000001 Rmu_sc0000957.1_g000012 Rmu_ssc0000295.1_g000013
rosa_roxburghii Rroxscaffold_5G00374660
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320200 Rh4BG328400 Rh4CG343800 Rh4DG323800
rosa_wichuraiana Rw0G008700 Rw4G027800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 1 cut(s) 1184
AclWI GGATC 4 cut(s) 56, 109, 585, 736
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 767, 945
AcuI CTGAAG 5 cut(s) 24, 107, 172, 728, 905
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 1415
AgsI TTSAA 5 cut(s) 374, 448, 494, 516, 1406
AjnI CCWGG 4 cut(s) 435, 982, 1132, 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 9 cut(s) 9, 151, 416, 751, 1008, 1158, 1308, 1444, 1525
AluI AGCT 9 cut(s) 9, 151, 416, 751, 1008, 1158, 1308, 1444, 1525
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 91, 214, 1115
AlwI GGATC 4 cut(s) 56, 109, 585, 736
Ama87I CYCGRG 1 cut(s) 1033
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 3 cut(s) 332, 1158, 1430
ApoI RAATTY 2 cut(s) 767, 945
Asp700I GAANNNNTTC 4 cut(s) 293, 946, 1028, 1178
Asp718I GGTACC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 866
AsuHPI GGTGA 1 cut(s) 844
AvaI CYCGRG 1 cut(s) 1033
AvaII GGWCC 1 cut(s) 866
AxyI CCTNAGG 1 cut(s) 871
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 3 cut(s) 11, 926, 1052
BbsI GAAGAC 4 cut(s) 30, 1001, 1151, 1301
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 319, 1145, 1417
BccI CCATC 5 cut(s) 305, 373, 782, 1063, 1363
BceAI ACGGC 3 cut(s) 5, 164, 600
BciT130I CCWGG 4 cut(s) 437, 984, 1134, 1284
BcoDI GTCTC 3 cut(s) 91, 214, 1115
BfaI CTAG 2 cut(s) 684, 953
BfmI CTRYAG 2 cut(s) 333, 746
BfuAI ACCTGC 1 cut(s) 479
BglII AGATCT 1 cut(s) 877
BisI GCNGC 4 cut(s) 216, 333, 1159, 1431
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 4 cut(s) 217, 334, 1160, 1432
Bme1390I CCNGG 4 cut(s) 437, 984, 1134, 1284
Bme18I GGWCC 1 cut(s) 866
BmeT110I CYCGRG 1 cut(s) 1033
BmgT120I GGNCC 1 cut(s) 866
BmiI GGNNCC 3 cut(s) 469, 867, 868
BmrFI CCNGG 4 cut(s) 437, 984, 1134, 1284
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 4 cut(s) 166, 688, 1214, 1508
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 4 cut(s) 30, 1001, 1151, 1301
BpmI CTGGAG 4 cut(s) 741, 966, 1116, 1266
Bpu1102I GCTNAGC 1 cut(s) 726
BsaBI GATNNNNATC 3 cut(s) 113, 702, 1506
BsaI GGTCTC 2 cut(s) 91, 214
BsaJI CCNNGG 2 cut(s) 573, 646
Bsc4I CCNNNNNNNGG 1 cut(s) 1415
Bse1I ACTGG 3 cut(s) 21, 719, 1428
Bse21I CCTNAGG 1 cut(s) 871
Bse8I GATNNNNATC 3 cut(s) 113, 702, 1506
BseBI CCWGG 4 cut(s) 437, 984, 1134, 1284
BseDI CCNNGG 2 cut(s) 573, 646
BseGI GGATG 2 cut(s) 604, 793
BseJI GATNNNNATC 3 cut(s) 113, 702, 1506
BseLI CCNNNNNNNGG 1 cut(s) 1415
BseMII CTCAG 5 cut(s) 24, 24, 166, 717, 885
BseNI ACTGG 3 cut(s) 21, 719, 1428
BseRI GAGGAG 2 cut(s) 663, 911
BseXI GCAGC 3 cut(s) 319, 1145, 1417
BseYI CCCAGC 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 1033
BsiSI CCGG 1 cut(s) 1416
BslFI GGGAC 1 cut(s) 879
BslI CCNNNNNNNGG 1 cut(s) 1415
BsmAI GTCTC 3 cut(s) 91, 214, 1115
BsmFI GGGAC 1 cut(s) 879
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 2 cut(s) 91, 214
BsoBI CYCGRG 1 cut(s) 1033
Bsp1286I GDGCHC 3 cut(s) 11, 926, 1052
Bsp143I GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
Bsp1720I GCTNAGC 1 cut(s) 726
Bsp68I TCGCGA 1 cut(s) 1184
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 5 cut(s) 23, 25, 165, 718, 884
BspFNI CGCG 1 cut(s) 1184
BspLI GGNNCC 3 cut(s) 469, 867, 868
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 4 cut(s) 56, 109, 585, 736
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 2 cut(s) 91, 214
BsrI ACTGG 3 cut(s) 21, 719, 1428
BssECI CCNNGG 2 cut(s) 573, 646
BssMI GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 4 cut(s) 437, 984, 1134, 1284
Bst4CI ACNGT 6 cut(s) 58, 795, 832, 1000, 1300, 1489
Bst6I CTCTTC 2 cut(s) 89, 396
BstC8I GCNNGC 4 cut(s) 7, 723, 1089, 1523
BstDEI CTNAG 6 cut(s) 10, 33, 152, 726, 871, 1228
BstEII GGTNACC 2 cut(s) 46, 832
BstF5I GGATG 2 cut(s) 604, 793
BstFNI CGCG 1 cut(s) 1184
BstKTI GATC 6 cut(s) 64, 117, 580, 744, 880, 1504
BstMAI GTCTC 3 cut(s) 91, 214, 1115
BstMBI GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
BstMWI GCNNNNNNNGC 5 cut(s) 50, 273, 413, 727, 1088
BstNI CCWGG 4 cut(s) 437, 984, 1134, 1284
BstPI GGTNACC 2 cut(s) 46, 832
BstSCI CCNGG 4 cut(s) 435, 982, 1132, 1282
BstSFI CTRYAG 2 cut(s) 333, 746
BstUI CGCG 1 cut(s) 1184
BstV1I GCAGC 3 cut(s) 319, 1145, 1417
BstV2I GAAGAC 4 cut(s) 30, 1001, 1151, 1301
BstX2I RGATCY 4 cut(s) 61, 114, 741, 877
BstYI RGATCY 4 cut(s) 61, 114, 741, 877
Bsu36I CCTNAGG 1 cut(s) 871
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 2 cut(s) 604, 793
BtsIMutI CAGTG 2 cut(s) 196, 1494
BtuMI TCGCGA 1 cut(s) 1184
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 4 cut(s) 7, 723, 1089, 1523
Cfr13I GGNCC 1 cut(s) 866
Csp6I GTAC 1 cut(s) 468
CviAII CATG 6 cut(s) 106, 317, 654, 665, 1096, 1246
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 6 cut(s) 10, 33, 152, 726, 871, 1228
DpnI GATC 6 cut(s) 63, 116, 579, 743, 879, 1503
DpnII GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 89, 396
EarI CTCTTC 2 cut(s) 89, 396
EciI GGCGGA 3 cut(s) 202, 575, 1434
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 3 cut(s) 11, 926, 1052
Eco31I GGTCTC 2 cut(s) 91, 214
Eco47I GGWCC 1 cut(s) 866
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 5 cut(s) 24, 107, 172, 728, 905
Eco81I CCTNAGG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 1033
Eco91I GGTNACC 2 cut(s) 46, 832
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 866
EcoO65I GGTNACC 2 cut(s) 46, 832
EcoRII CCWGG 4 cut(s) 435, 982, 1132, 1282
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 3 cut(s) 11, 926, 1052
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 6 cut(s) 109, 320, 657, 668, 1099, 1249
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 1060, 1092
FaqI GGGAC 1 cut(s) 879
FatI CATG 6 cut(s) 105, 316, 653, 664, 1095, 1245
FauI CCCGC 1 cut(s) 1210
Fnu4HI GCNGC 4 cut(s) 216, 333, 1159, 1431
FokI GGATG 2 cut(s) 611, 800
FriOI GRGCYC 3 cut(s) 11, 926, 1052
Fsp4HI GCNGC 4 cut(s) 216, 333, 1159, 1431
FspBI CTAG 2 cut(s) 684, 953
GluI GCNGC 4 cut(s) 216, 333, 1159, 1431
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 4 cut(s) 741, 966, 1116, 1266
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 1416
Hin1II CATG 6 cut(s) 109, 320, 657, 668, 1099, 1249
HinfI GANTC 6 cut(s) 35, 1071, 1106, 1123, 1256, 1402
HpaII CCGG 1 cut(s) 1416
HphI GGTGA 1 cut(s) 844
Hpy166II GTNNAC 2 cut(s) 1243, 1450
Hpy188I TCNGA 9 cut(s) 13, 34, 87, 155, 426, 708, 787, 855, 1517
Hpy8I GTNNAC 2 cut(s) 1243, 1450
HpyAV CCTTC 6 cut(s) 283, 322, 488, 703, 880, 936
HpyCH4III ACNGT 6 cut(s) 58, 795, 832, 1000, 1300, 1489
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 7 cut(s) 130, 335, 358, 388, 531, 1161, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 273, 413, 727, 1088
HpyF3I CTNAG 6 cut(s) 10, 33, 152, 726, 871, 1228
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 6 cut(s) 109, 320, 657, 668, 1099, 1249
KflI GGGWCCC 1 cut(s) 866
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
LmnI GCTCC 2 cut(s) 650, 1441
Lsp1109I GCAGC 3 cut(s) 319, 1145, 1417
LweI GCATC 4 cut(s) 166, 688, 1214, 1508
MaeI CTAG 2 cut(s) 684, 953
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 46, 478, 832, 936
MalI GATC 6 cut(s) 63, 116, 579, 743, 879, 1503
MboI GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
MfeI CAATTG 1 cut(s) 1528
MflI RGATCY 4 cut(s) 61, 114, 741, 877
MhlI GDGCHC 3 cut(s) 11, 926, 1052
MluCI AATT 8 cut(s) 135, 352, 489, 767, 860, 945, 1497, 1528
MlyI GAGTC 2 cut(s) 44, 1117
MroXI GAANNNNTTC 4 cut(s) 293, 946, 1028, 1178
MseI TTAA 6 cut(s) 138, 411, 488, 957, 966, 1302
MslI CAYNNNNRTG 2 cut(s) 1487, 1490
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 1 cut(s) 1416
MspR9I CCNGG 4 cut(s) 437, 984, 1134, 1284
MunI CAATTG 1 cut(s) 1528
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 4 cut(s) 437, 984, 1134, 1284
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 5 cut(s) 50, 273, 413, 727, 1088
NdeII GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
NlaIII CATG 6 cut(s) 109, 320, 657, 668, 1099, 1249
NlaIV GGNNCC 3 cut(s) 469, 867, 868
NmuCI GTSAC 2 cut(s) 478, 832
NruI TCGCGA 1 cut(s) 1184
PcsI WCGNNNNNNNCGW 1 cut(s) 1260
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 4 cut(s) 293, 946, 1028, 1178
PfeI GAWTC 4 cut(s) 1071, 1106, 1256, 1402
PkrI GCNGC 4 cut(s) 217, 334, 1160, 1432
PleI GAGTC 2 cut(s) 43, 1117
PpsI GAGTC 2 cut(s) 43, 1117
PpuMI RGGWCCY 1 cut(s) 866
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 866
Psp6I CCWGG 4 cut(s) 435, 982, 1132, 1282
PspEI GGTNACC 2 cut(s) 46, 832
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 4 cut(s) 435, 982, 1132, 1282
PspN4I GGNNCC 3 cut(s) 469, 867, 868
PspPI GGNCC 1 cut(s) 866
PspPPI RGGWCCY 1 cut(s) 866
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 4 cut(s) 61, 114, 741, 877
RruI TCGCGA 1 cut(s) 1184
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 2 cut(s) 1487, 1490
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 6 cut(s) 138, 411, 488, 957, 966, 1302
SatI GCNGC 4 cut(s) 216, 333, 1159, 1431
Sau3AI GATC 6 cut(s) 61, 114, 577, 741, 877, 1501
Sau96I GGNCC 1 cut(s) 866
SchI GAGTC 2 cut(s) 44, 1117
ScrFI CCNGG 4 cut(s) 437, 984, 1134, 1284
SduI GDGCHC 3 cut(s) 11, 926, 1052
SfaNI GCATC 4 cut(s) 166, 688, 1214, 1508
SfcI CTRYAG 2 cut(s) 333, 746
SinI GGWCC 1 cut(s) 866
SmiMI CAYNNNNRTG 2 cut(s) 1487, 1490
Sse9I AATT 8 cut(s) 135, 352, 489, 767, 860, 945, 1497, 1528
SspI AATATT 1 cut(s) 367
SspMI CTAG 2 cut(s) 684, 953
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 435, 982, 1132, 1282
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 6 cut(s) 58, 795, 832, 1000, 1300, 1489
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 1058, 1208
TasI AATT 8 cut(s) 135, 352, 489, 767, 860, 945, 1497, 1528
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 4 cut(s) 1071, 1106, 1256, 1402
Tru1I TTAA 6 cut(s) 138, 411, 488, 957, 966, 1302
Tru9I TTAA 6 cut(s) 138, 411, 488, 957, 966, 1302
TscAI CASTG 2 cut(s) 196, 1494
TseFI GTSAC 2 cut(s) 478, 832
TseI GCWGC 3 cut(s) 332, 1158, 1430
Tsp45I GTSAC 2 cut(s) 478, 832
TspDTI ATGAA 9 cut(s) 615, 653, 918, 1112, 1119, 1209, 1262, 1359, 1412
TspRI CASTG 2 cut(s) 196, 1494
VpaK11BI GGWCC 1 cut(s) 866
XapI RAATTY 2 cut(s) 767, 945
XbaI TCTAGA 1 cut(s) 683
XmnI GAANNNNTTC 4 cut(s) 293, 946, 1028, 1178
XspI CTAG 2 cut(s) 684, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.