AT1G73480

hydrolase, alpha beta fold family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010185
Physical Location & Seq
Forward (+)
27628678 .. 27632840
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73480.1

Sequence Viewer

Length: 1392 bp
ATGATCTGTTCAAGTAAAGGAACAATCTTAATCGCTAGAGGTAAAAATTCAAATACAATCCCTAGATTACCCCTAAAAGGGTCATCCACAAATTACGCGAATCGAATTCAAATTCGAAACCCAATCAGTGTCAAAACTTTCCGGCGATCATCTTCATCGTCAATGGCGGTGGAAACAATGTCGATGGGATCAGATTCATCAACTTTGATTCTAACATCAGGAGCAAGCGGTCGCGTTAGGGTACTCTTCTCGATGCGAGAGCTTAAGCGTCTCGTTACGATTATCCAATCGTTGATTCTTTTCCTCCTCCTTCCGTTTCGCGTCGTCGTTTGGCGGCGGAGGACTGGTGCGGTGGTTATCAGAGACGATAAGCAAGAGAGGAAGGTTTGGTCTCCTCCGCAGATCGTGGTGAGGAAGAGGAACATCGGTGGCGAAAGCAGCGTTTCTCCTCCGTCGGTTCCAGCTGCGGTGGTGGATGGGGAGGTTGCTGTTCGACGTGAACTGGCGATTAAGCGAGTTTTGGAGGATGAAGGCGGCGATGGAAGCTCCGTCAGAGATTATTCGCTATTCACGACGAAGAGAGGAGATACGTTGTTTAGTCAGTCATGGTCACCTCTTTCCCCAAATCACAGGGGACTTATTGTTCTGCTACATGGATTAAACGAGCATAGTGGCAGGTATAGTGATTTTGCAAAGCAGCTTAATGCTAATGGGTTCAAGGTCTATGGAATTGACTGGATCGGTCATGGCGGAAGTGATGGACTTCATGCTTACGTTCCTTCCCTTGATTACGCTGTCACAGATTTGAAATCATTTCTTGAAAAGGTATTCACAGAGAATCCAGGACTCCCCTGTTTCTGCTTTGGACACTCAACAGGTGGAGCAATCATCCTCAAGGCTATGCTGGATCCAAAGATTGAATCTCGAGTTTCAGGCATTGCATTGACTTCACCAGCTGTTGGAGTCCAACCATCCCATCCAATCTTCGCTGTTCTTGCTCCAATCATGGCGTTTCTACTACCCAGGTACCAAATCAGTGCAGCAAACAAGAAAGGAATGCCGGTTTCTCGTGACCCAGCAGCTCTCATCGCCAAATACTCTGACCCATTAGTCTTCACCGGATCCATCCGGGTTAAAACCGGCTACGAGATCCTTAGAATCACTGCTCACTTGCAACAGAACCTGAACAAAGTGAAAGTTCCCTTTCTTGTGATGCACGGTACTGACGACACAGTTACCGATCCTAGCGCCTCAAAGAAGCTCTACGAGGAAGCTGCCTCGTCAGACAAATCACTCAAGCTCTACGACGGGTTGTTGCACGATCTTCTTTTTGAACCCGAACGAGAAATCATCGCTGGAGCCATATTAGATTGGCTAAACCAGCGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

463

Amino Acids

50.77

Weight (kDa)

9.75

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 209 - 447 3.2e-77 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 213 - 341 3.2e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 214 - 446 1.4e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1109
Acc36I ACCTGC 1 cut(s) 666
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 1026
AccB7I CCANNNNNTGG 1 cut(s) 959
AccII CGCG 3 cut(s) 98, 234, 321
AclWI GGATC 8 cut(s) 196, 746, 902, 915, 1116, 1129, 1144, 1235
AcsI RAATTY 3 cut(s) 46, 105, 111
AfaI GTAC 3 cut(s) 243, 1028, 1222
AfiI CCNNNNNNNGG 3 cut(s) 77, 78, 959
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 8 cut(s) 12, 51, 110, 718, 808, 821, 920, 1334
AjiI CACGTC 1 cut(s) 497
AjnI CCWGG 2 cut(s) 841, 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AloI GAACNNNNNNTCC 2 cut(s) 627, 659
AluBI AGCT 9 cut(s) 262, 464, 546, 700, 956, 1082, 1261, 1274, 1300
AluI AGCT 9 cut(s) 262, 464, 546, 700, 956, 1082, 1261, 1274, 1300
Alw26I GTCTC 3 cut(s) 275, 357, 396
AlwI GGATC 8 cut(s) 196, 746, 902, 915, 1116, 1129, 1144, 1235
AlwNI CAGNNNCTG 1 cut(s) 1183
Ama87I CYCGRG 1 cut(s) 924
ApeKI GCWGC 6 cut(s) 438, 464, 697, 1040, 1079, 1274
ApoI RAATTY 3 cut(s) 46, 105, 111
Asp718I GGTACC 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 1250
AsuC2I CCSGG 1 cut(s) 1130
AsuHPI GGTGA 4 cut(s) 421, 603, 942, 1108
AsuII TTCGAA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 924
BaeI ACNNNNGTAYC 2 cut(s) 1010, 1043
BamHI GGATCC 2 cut(s) 907, 1121
BanI GGYRCC 1 cut(s) 1026
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 1105
BbvI GCAGC 6 cut(s) 450, 451, 709, 1052, 1091, 1261
BccI CCATC 7 cut(s) 178, 470, 533, 752, 979, 984, 1133
BcgI CGANNNNNNTGC 2 cut(s) 1256, 1290
BciT130I CCWGG 2 cut(s) 843, 1024
BcnI CCSGG 1 cut(s) 1130
BcoDI GTCTC 3 cut(s) 275, 357, 396
BfaI CTAG 3 cut(s) 36, 63, 1245
BfoI RGCGCY 1 cut(s) 1251
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 666
BisI GCNGC 8 cut(s) 335, 439, 465, 535, 698, 1041, 1080, 1275
BlsI GCNGC 8 cut(s) 336, 440, 466, 536, 699, 1042, 1081, 1276
Bme1390I CCNGG 3 cut(s) 843, 1024, 1130
BmeT110I CYCGRG 1 cut(s) 924
BmgBI CACGTC 1 cut(s) 497
BmiI GGNNCC 5 cut(s) 459, 909, 1028, 1123, 1360
BmrFI CCNGG 3 cut(s) 843, 1024, 1130
BmsI GCATC 2 cut(s) 243, 1203
BpiI GAAGAC 1 cut(s) 1105
BpmI CTGGAG 1 cut(s) 1377
Bpu14I TTCGAA 1 cut(s) 115
BpuEI CTTGAG 2 cut(s) 878, 1280
BpuMI CCSGG 1 cut(s) 1130
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 1022
BsaWI WCCGGW 1 cut(s) 1118
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 78, 959
Bse118I RCCGGY 2 cut(s) 1060, 1139
Bse1I ACTGG 3 cut(s) 349, 507, 740
Bse3DI GCAATG 1 cut(s) 936
BseBI CCWGG 2 cut(s) 843, 1024
BseDI CCNNGG 1 cut(s) 1022
BseGI GGATG 7 cut(s) 83, 481, 532, 888, 971, 976, 1125
BseLI CCNNNNNNNGG 3 cut(s) 77, 78, 959
BseMI GCAATG 1 cut(s) 936
BseNI ACTGG 3 cut(s) 349, 507, 740
BseRI GAGGAG 4 cut(s) 296, 384, 438, 597
BseXI GCAGC 6 cut(s) 450, 451, 709, 1052, 1091, 1261
BseYI CCCAGC 1 cut(s) 1075
BsgI GTGCAG 1 cut(s) 1059
Bsh1236I CGCG 3 cut(s) 98, 234, 321
Bsh1285I CGRYCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 1026
BsiEI CGRYCG 1 cut(s) 232
BsiHKCI CYCGRG 1 cut(s) 924
BsiSI CCGG 5 cut(s) 142, 1061, 1119, 1129, 1140
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 3 cut(s) 77, 78, 959
BsmAI GTCTC 3 cut(s) 275, 357, 396
BsmBI CGTCTC 2 cut(s) 275, 357
BsmFI GGGAC 1 cut(s) 648
BsmI GAATGC 1 cut(s) 1062
Bso31I GGTCTC 1 cut(s) 396
BsoBI CYCGRG 1 cut(s) 924
Bsp119I TTCGAA 1 cut(s) 115
BspFNI CGCG 3 cut(s) 98, 234, 321
BspLI GGNNCC 5 cut(s) 459, 909, 1028, 1123, 1360
BspMI ACCTGC 1 cut(s) 666
BspPI GGATC 8 cut(s) 196, 746, 902, 915, 1116, 1129, 1144, 1235
BspT104I TTCGAA 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 1026
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 936
BsrFI RCCGGY 2 cut(s) 1060, 1139
BsrI ACTGG 3 cut(s) 349, 507, 740
BssAI RCCGGY 2 cut(s) 1060, 1139
BssECI CCNNGG 1 cut(s) 1022
BssSI CACGAG 1 cut(s) 1068
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 2 cut(s) 843, 1024
Bst4CI ACNGT 2 cut(s) 1220, 1234
Bst6I CTCTTC 3 cut(s) 251, 410, 572
BstAFI CTTAAG 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 1154
BstEII GGTNACC 1 cut(s) 609
BstF5I GGATG 7 cut(s) 83, 481, 532, 888, 971, 976, 1125
BstFNI CGCG 3 cut(s) 98, 234, 321
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 1 cut(s) 1250
BstMAI GTCTC 3 cut(s) 275, 357, 396
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 5 cut(s) 438, 543, 995, 1088, 1381
BstNI CCWGG 2 cut(s) 843, 1024
BstPI GGTNACC 1 cut(s) 609
BstSCI CCNGG 3 cut(s) 841, 1022, 1128
BstUI CGCG 3 cut(s) 98, 234, 321
BstV1I GCAGC 6 cut(s) 450, 451, 709, 1052, 1091, 1261
BstV2I GAAGAC 1 cut(s) 1105
BstX2I RGATCY 3 cut(s) 907, 1121, 1149
BstYI RGATCY 3 cut(s) 907, 1121, 1149
BtgZI GCGATG 3 cut(s) 552, 1072, 1336
BtrI CACGTC 1 cut(s) 497
BtsCI GGATG 7 cut(s) 83, 481, 532, 888, 971, 976, 1125
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 3 cut(s) 133, 1042, 1161
BveI ACCTGC 1 cut(s) 666
Cac8I GCNNGC 1 cut(s) 226
CaiI CAGNNNCTG 1 cut(s) 1183
CfoI GCGC 1 cut(s) 1250
Cfr10I RCCGGY 2 cut(s) 1060, 1139
CseI GACGC 2 cut(s) 257, 310
Csp6I GTAC 3 cut(s) 242, 1027, 1221
CspCI CAANNNNNGTGG 2 cut(s) 150, 185
CviAII CATG 5 cut(s) 606, 653, 746, 767, 1006
CviQI GTAC 3 cut(s) 242, 1027, 1221
DdeI CTNAG 1 cut(s) 1154
DrdI GACNNNNNNGTC 1 cut(s) 1109
DseDI GACNNNNNNGTC 1 cut(s) 1109
Eam1104I CTCTTC 3 cut(s) 251, 410, 572
EarI CTCTTC 3 cut(s) 251, 410, 572
EciI GGCGGA 2 cut(s) 352, 765
Eco31I GGTCTC 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 924
Eco91I GGTNACC 1 cut(s) 609
EcoO65I GGTNACC 1 cut(s) 609
EcoRI GAATTC 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 841, 1022
Esp3I CGTCTC 2 cut(s) 275, 357
FaeI CATG 5 cut(s) 609, 656, 749, 770, 1009
FaqI GGGAC 1 cut(s) 648
FatI CATG 5 cut(s) 605, 652, 745, 766, 1005
FauI CCCGC 1 cut(s) 1377
Fnu4HI GCNGC 8 cut(s) 335, 439, 465, 535, 698, 1041, 1080, 1275
FokI GGATG 7 cut(s) 70, 488, 539, 875, 958, 963, 1112
Fsp4HI GCNGC 8 cut(s) 335, 439, 465, 535, 698, 1041, 1080, 1275
FspBI CTAG 3 cut(s) 36, 63, 1245
GlaI GCGC 1 cut(s) 1249
GluI GCNGC 8 cut(s) 335, 439, 465, 535, 698, 1041, 1080, 1275
GsaI CCCAGC 1 cut(s) 1079
GsuI CTGGAG 1 cut(s) 1377
HaeII RGCGCY 1 cut(s) 1251
HapII CCGG 5 cut(s) 142, 1061, 1119, 1129, 1140
HgaI GACGC 2 cut(s) 257, 310
HhaI GCGC 1 cut(s) 1250
Hin1II CATG 5 cut(s) 609, 656, 749, 770, 1009
Hin6I GCGC 1 cut(s) 1248
HinP1I GCGC 1 cut(s) 1248
HinfI GANTC 9 cut(s) 100, 194, 208, 295, 838, 846, 920, 963, 1158
HpaII CCGG 5 cut(s) 142, 1061, 1119, 1129, 1140
HphI GGTGA 4 cut(s) 421, 603, 942, 1108
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 5 cut(s) 193, 362, 554, 1102, 1285
Hpy188III TCNNGA 6 cut(s) 219, 250, 571, 818, 924, 1070
Hpy8I GTNNAC 1 cut(s) 500
Hpy99I CGWCG 6 cut(s) 326, 329, 457, 498, 577, 1310
HpyAV CCTTC 4 cut(s) 320, 376, 524, 789
HpyCH4III ACNGT 2 cut(s) 1220, 1234
HpyCH4IV ACGT 3 cut(s) 496, 590, 774
HpyCH4V TGCA 6 cut(s) 692, 941, 1040, 1174, 1216, 1318
HpyF10VI GCNNNNNNNGC 5 cut(s) 438, 543, 995, 1088, 1381
HpyF3I CTNAG 1 cut(s) 1154
HpySE526I ACGT 3 cut(s) 496, 590, 774
Hsp92II CATG 5 cut(s) 609, 656, 749, 770, 1009
HspAI GCGC 1 cut(s) 1248
KpnI GGTACC 1 cut(s) 1030
LmnI GCTCC 5 cut(s) 221, 551, 881, 1003, 1358
Lsp1109I GCAGC 6 cut(s) 450, 451, 709, 1052, 1091, 1261
LweI GCATC 2 cut(s) 243, 1203
MaeI CTAG 3 cut(s) 36, 63, 1245
MaeII ACGT 3 cut(s) 496, 590, 774
MaeIII GTNAC 5 cut(s) 274, 609, 796, 1070, 1234
MboII GAAGA 7 cut(s) 144, 238, 427, 589, 976, 1105, 1316
MflI RGATCY 3 cut(s) 907, 1121, 1149
MluCI AATT 5 cut(s) 46, 91, 105, 111, 729
MlyI GAGTC 2 cut(s) 840, 972
MmeI TCCRAC 2 cut(s) 940, 991
MseI TTAA 6 cut(s) 29, 264, 510, 659, 702, 1134
MspA1I CMGCKG 3 cut(s) 464, 956, 1384
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 5 cut(s) 142, 1061, 1119, 1129, 1140
MspR9I CCNGG 3 cut(s) 843, 1024, 1130
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 843, 1024
MvnI CGCG 3 cut(s) 98, 234, 321
MwoI GCNNNNNNNGC 5 cut(s) 438, 543, 995, 1088, 1381
NciI CCSGG 1 cut(s) 1130
NlaIII CATG 5 cut(s) 609, 656, 749, 770, 1009
NlaIV GGNNCC 5 cut(s) 459, 909, 1028, 1123, 1360
NmuCI GTSAC 3 cut(s) 609, 796, 1070
NspV TTCGAA 1 cut(s) 115
PaeR7I CTCGAG 1 cut(s) 924
PcsI WCGNNNNNNNCGW 2 cut(s) 569, 1224
PctI GAATGC 1 cut(s) 1062
PfeI GAWTC 7 cut(s) 100, 194, 208, 295, 838, 920, 1158
PflMI CCANNNNNTGG 1 cut(s) 959
PfoI TCCNGGA 1 cut(s) 841
PkrI GCNGC 8 cut(s) 336, 440, 466, 536, 699, 1042, 1081, 1276
PleI GAGTC 2 cut(s) 840, 971
PpsI GAGTC 2 cut(s) 840, 971
Psp6I CCWGG 2 cut(s) 841, 1022
PspEI GGTNACC 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 1075
PspGI CCWGG 2 cut(s) 841, 1022
PspN4I GGNNCC 5 cut(s) 459, 909, 1028, 1123, 1360
PstNI CAGNNNCTG 1 cut(s) 1183
PsuI RGATCY 3 cut(s) 907, 1121, 1149
PvuII CAGCTG 2 cut(s) 464, 956
RsaI GTAC 3 cut(s) 243, 1028, 1222
RsaNI GTAC 3 cut(s) 242, 1027, 1221
SaqAI TTAA 6 cut(s) 29, 264, 510, 659, 702, 1134
SatI GCNGC 8 cut(s) 335, 439, 465, 535, 698, 1041, 1080, 1275
SchI GAGTC 2 cut(s) 840, 972
ScrFI CCNGG 3 cut(s) 843, 1024, 1130
SfaNI GCATC 2 cut(s) 243, 1203
Sfr274I CTCGAG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 115
SlaI CTCGAG 1 cut(s) 924
SmlI CTYRAG 4 cut(s) 263, 893, 924, 1295
SmoI CTYRAG 4 cut(s) 263, 893, 924, 1295
Sse9I AATT 5 cut(s) 46, 91, 105, 111, 729
SspMI CTAG 3 cut(s) 36, 63, 1245
StyD4I CCNGG 3 cut(s) 841, 1022, 1128
TaaI ACNGT 2 cut(s) 1220, 1234
TaiI ACGT 3 cut(s) 499, 593, 777
TaqI TCGA 6 cut(s) 103, 115, 182, 251, 493, 925
TaqII GACCGA 1 cut(s) 731
TasI AATT 5 cut(s) 46, 91, 105, 111, 729
TauI GCSGC 2 cut(s) 337, 537
TfiI GAWTC 7 cut(s) 100, 194, 208, 295, 838, 920, 1158
Tru1I TTAA 6 cut(s) 29, 264, 510, 659, 702, 1134
Tru9I TTAA 6 cut(s) 29, 264, 510, 659, 702, 1134
TscAI CASTG 3 cut(s) 133, 1042, 1168
TseFI GTSAC 3 cut(s) 609, 796, 1070
TseI GCWGC 6 cut(s) 438, 464, 697, 1040, 1079, 1274
Tsp45I GTSAC 3 cut(s) 609, 796, 1070
TspDTI ATGAA 4 cut(s) 144, 186, 543, 755
TspGWI ACGGA 3 cut(s) 303, 441, 538
TspRI CASTG 3 cut(s) 133, 1042, 1168
Van91I CCANNNNNTGG 1 cut(s) 959
Vha464I CTTAAG 1 cut(s) 263
XapI RAATTY 3 cut(s) 46, 105, 111
XhoI CTCGAG 1 cut(s) 924
XspI CTAG 3 cut(s) 36, 63, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.