MD14G1240600.v1.1

Monoglyceride lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010185
Physical Location & Seq
Forward (+)
31950828 .. 31955830
5003 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1240600.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGATCTGTTTGGGAAAGGCAGGGATTATAGATAGTAGAATTCTGTTTTTTCACGGGGAGAAGAAGGAGCCAAACAACAACCTCAACAGGAAACAACCAATCCGGCCGTTGATTGGAGTGGAGGAGCTCCAATTCCATTTCAACTCGTCATCTCCACCGCCGCCGGCGTCATCAGCATTGTTCTTTCAACCCACAAGACCACCGCCGAAGAAGCAGCAGAAAATGGCGACGGAGAGAGGCAGCGATTTGTCGTCGTCGTCGTCGTTGATGTTGACTTCTGGAGCGAGCGGCCGCATAAACGCCCTCCTCTCGCTGCGGGCGCTGCGGAGTCTGATGATGCTGATTAACGCCTTCTTCCTGCTCCTTCTGCTCCCATTTCGCGGCCGCAAGCGAGCGCCGTCGTCGTCCGCCTCGTCGTCTGCGGCGGCGGCTTCGCCGGTCTTGGCGGAGAAGTCGGCCAAGGACGAGAGGCAGCCTAGGGGGCCCGTGGTCCGGGTGCCAACAACGATTGTTCCGTGGAAGAGCAGCTCGTCGTTGCCGGTGGACCCGGAGGTGGCGGCGAGGAGAGCGTTAGCGAAGCTAAGAGTTGTACAAGATGATGATGAGAATTCGGTCCGGCGATATTCGCTCTTTGCGACTGCGAGAGGTGAAACTCTCTTCACGCAAAGTTGGACTCCCGTTTCCGTTAACATCAGGGGCGTAGTCATTCTTATGCATGGTCTGAACGAACACAGTGGAAGATACGATTACTTTGCAAAGCAACTGAATGCTAATGGATTTAAGGTTTACGGAATGGATTGGATTGGTCATGGTGGGAGTGATGGGCTTCATGCATATGTTCCTTCTCTCGATTATGCTGTTACTGATATGAAATTATTTCTCGAAAAGGTTTTAGCTGAAAATCCAGGCCTACCGTGTTTCTGTTTTGGGCATTCCACAGGTGCAGCCATTGTCCTTAAGGTAATGCTTGACCCAAAATTTGAGTCCCGCATAGACGGTGTAGTGCTCACATCACCAGCAGTTGGGGTTCAGCCTACGCATCCAATATTTGTGGTAATTGCTCCATTGCTCTCATTTTTGCTGCCAAGATACCAAATCAGTGCAGCAAATAAGAAGGGCATGCCTGTTTCTCGTGACCCAGCGGCTCTAGTTGCCAAGTATTCGGACCCTCTAGTGTACACTGGATCCATCCGCGTAAGGACTGGTTATGAGATTCTCCGAATCACATCCTACTTGCAGCAGAACCTAACCAAATTGAGGGTCCCCTTCTTTGTTCTCCACGGCACAGCTGATACGGTTACTGACCCCGAGGCCTCCCAGAAACTGTACGATGAAGCCTCCTCAACTGACAAAACCATCAAACTGTTTGACGGGTTGTTACATGACCTTCTCTTTGAGCCAGAGCGAGAAGCCATTACGAAGGGCATCATTGAGTGGTTGAATCGTAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

485

Amino Acids

53.3

Weight (kDa)

9.63

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 231 - 468 2e-77 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 234 - 343 1.6e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 235 - 465 8.8e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 496
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccBSI CCGCTC 1 cut(s) 288
AccII CGCG 2 cut(s) 381, 1194
AclWI GGATC 2 cut(s) 1179, 1192
AcoI YGGCCR 4 cut(s) 104, 289, 382, 456
AcsI RAATTY 3 cut(s) 39, 607, 977
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 3 cut(s) 591, 1178, 1328
AfiI CCNNNNNNNGG 6 cut(s) 113, 380, 492, 553, 1022, 1257
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 3 cut(s) 142, 188, 1441
AjnI CCWGG 1 cut(s) 904
AluBI AGCT 5 cut(s) 127, 528, 580, 896, 1289
AluI AGCT 5 cut(s) 127, 528, 580, 896, 1289
Alw21I GWGCWC 2 cut(s) 129, 1008
AlwI GGATC 2 cut(s) 1179, 1192
AlwNI CAGNNNCTG 1 cut(s) 1324
Ama87I CYCGRG 1 cut(s) 1307
AoxI GGCC 7 cut(s) 104, 289, 382, 456, 482, 907, 1311
ApaI GGGCCC 1 cut(s) 486
ApoI RAATTY 3 cut(s) 39, 607, 977
ArsI GACNNNNNNTTYG 8 cut(s) 962, 968, 994, 1000, 1245, 1277, 1376, 1408
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 2 cut(s) 322, 397
AspS9I GGNCC 7 cut(s) 482, 483, 490, 544, 613, 1165, 1261
AsuC2I CCSGG 2 cut(s) 494, 548
AsuHPI GGTGA 2 cut(s) 659, 1005
AvaI CYCGRG 1 cut(s) 1307
AvaII GGWCC 5 cut(s) 490, 544, 613, 1165, 1261
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 486
BamHI GGATCC 1 cut(s) 1184
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 2 cut(s) 129, 486
BauI CACGAG 1 cut(s) 1131
Bbv12I GWGCWC 2 cut(s) 129, 1008
BccI CCATC 3 cut(s) 815, 1196, 1364
BceAI ACGGC 3 cut(s) 91, 382, 1297
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 1 cut(s) 906
BcnI CCSGG 2 cut(s) 494, 548
BfaI CTAG 3 cut(s) 477, 1148, 1172
BfoI RGCGCY 2 cut(s) 323, 398
BfrI CTTAAG 1 cut(s) 956
BglI GCCNNNNNGGC 1 cut(s) 481
BlnI CCTAGG 1 cut(s) 476
Bme1390I CCNGG 3 cut(s) 494, 548, 906
Bme18I GGWCC 5 cut(s) 490, 544, 613, 1165, 1261
BmeT110I CYCGRG 1 cut(s) 1307
BmgT120I GGNCC 7 cut(s) 482, 483, 490, 544, 613, 1165, 1261
BmiI GGNNCC 9 cut(s) 69, 483, 484, 498, 546, 1167, 1186, 1262, 1263
BmrFI CCNGG 3 cut(s) 494, 548, 906
BmsI GCATC 3 cut(s) 327, 1048, 1434
BpmI CTGGAG 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 494, 548
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 6 cut(s) 459, 476, 486, 515, 1279, 1308
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 380, 492, 553, 1022, 1257
Bse118I RCCGGY 3 cut(s) 163, 436, 538
Bse1I ACTGG 2 cut(s) 1186, 1207
Bse3DI GCAATG 1 cut(s) 1064
BseBI CCWGG 1 cut(s) 906
BseDI CCNNGG 6 cut(s) 459, 476, 486, 515, 1279, 1308
BseGI GGATG 3 cut(s) 1039, 1188, 1226
BseLI CCNNNNNNNGG 6 cut(s) 113, 380, 492, 553, 1022, 1257
BseMI GCAATG 1 cut(s) 1064
BseNI ACTGG 2 cut(s) 1186, 1207
BseRI GAGGAG 4 cut(s) 137, 296, 577, 1330
BseSI GKGCMC 1 cut(s) 486
BseX3I CGGCCG 3 cut(s) 104, 289, 382
BseYI CCCAGC 1 cut(s) 1138
BsgI GTGCAG 2 cut(s) 963, 1122
Bsh1236I CGCG 2 cut(s) 381, 1194
Bsh1285I CGRYCG 3 cut(s) 107, 292, 385
BshFI GGCC 7 cut(s) 106, 291, 384, 458, 484, 909, 1313
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 3 cut(s) 107, 292, 385
BsiHKAI GWGCWC 2 cut(s) 129, 1008
BsiHKCI CYCGRG 1 cut(s) 1307
BsiSI CCGG 7 cut(s) 103, 164, 437, 493, 539, 548, 616
BslFI GGGAC 2 cut(s) 970, 1247
BslI CCNNNNNNNGG 6 cut(s) 113, 380, 492, 553, 1022, 1257
BsmFI GGGAC 2 cut(s) 970, 1247
BsmI GAATGC 2 cut(s) 772, 931
BsnI GGCC 7 cut(s) 106, 291, 384, 458, 484, 909, 1313
BsoBI CYCGRG 1 cut(s) 1307
Bsp120I GGGCCC 1 cut(s) 482
Bsp1286I GDGCHC 3 cut(s) 129, 486, 1008
Bsp1407I TGTACA 2 cut(s) 589, 1176
Bsp143I GATC 2 cut(s) 3, 1184
BspANI GGCC 7 cut(s) 106, 291, 384, 458, 484, 909, 1313
BspFNI CGCG 2 cut(s) 381, 1194
BspLI GGNNCC 9 cut(s) 69, 483, 484, 498, 546, 1167, 1186, 1262, 1263
BspPI GGATC 2 cut(s) 1179, 1192
BspQI GCTCTTC 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 496
BspTI CTTAAG 1 cut(s) 956
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 1064
BsrFI RCCGGY 3 cut(s) 163, 436, 538
BsrGI TGTACA 2 cut(s) 589, 1176
BsrI ACTGG 2 cut(s) 1186, 1207
BssAI RCCGGY 3 cut(s) 163, 436, 538
BssECI CCNNGG 6 cut(s) 459, 476, 486, 515, 1279, 1308
BssMI GATC 2 cut(s) 3, 1184
BssNI GRCGYC 1 cut(s) 167
BssSI CACGAG 1 cut(s) 1131
BssT1I CCWWGG 2 cut(s) 459, 476
Bst2BI CACGAG 1 cut(s) 1131
Bst2UI CCWGG 1 cut(s) 906
Bst4CI ACNGT 6 cut(s) 734, 915, 998, 1297, 1326, 1365
Bst6I CTCTTC 2 cut(s) 515, 662
BstACI GRCGYC 1 cut(s) 167
BstAFI CTTAAG 1 cut(s) 956
BstAUI TGTACA 2 cut(s) 589, 1176
BstC8I GCNNGC 6 cut(s) 165, 286, 318, 389, 393, 1121
BstDEI CTNAG 1 cut(s) 581
BstDSI CCRYGG 3 cut(s) 486, 515, 1279
BstF5I GGATG 3 cut(s) 1039, 1188, 1226
BstFNI CGCG 2 cut(s) 381, 1194
BstH2I RGCGCY 2 cut(s) 323, 398
BstHHI GCGC 2 cut(s) 322, 397
BstKTI GATC 2 cut(s) 6, 1187
BstMBI GATC 2 cut(s) 3, 1184
BstMCI CGRYCG 3 cut(s) 107, 292, 385
BstNI CCWGG 1 cut(s) 906
BstNSI RCATGY 1 cut(s) 1123
BstSCI CCNGG 3 cut(s) 492, 546, 904
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 2 cut(s) 381, 1194
BstX2I RGATCY 1 cut(s) 1184
BstYI RGATCY 1 cut(s) 1184
BstZI CGGCCG 3 cut(s) 104, 289, 382
BsuRI GGCC 7 cut(s) 106, 291, 384, 458, 484, 909, 1313
BtgI CCRYGG 3 cut(s) 486, 515, 1279
BtsCI GGATG 3 cut(s) 1039, 1188, 1226
BtsIMutI CAGTG 3 cut(s) 739, 1105, 1179
Cac8I GCNNGC 6 cut(s) 165, 286, 318, 389, 393, 1121
CaiI CAGNNNCTG 1 cut(s) 1324
CciNI GCGGCCGC 2 cut(s) 289, 382
CfoI GCGC 2 cut(s) 322, 397
Cfr10I RCCGGY 3 cut(s) 163, 436, 538
Cfr13I GGNCC 7 cut(s) 482, 483, 490, 544, 613, 1165, 1261
CpoI CGGWCCG 1 cut(s) 613
CseI GACGC 1 cut(s) 156
Csp6I GTAC 3 cut(s) 590, 1177, 1327
CspCI CAANNNNNGTGG 2 cut(s) 1032, 1067
CspI CGGWCCG 1 cut(s) 613
CviAII CATG 5 cut(s) 716, 809, 830, 1120, 1382
CviQI GTAC 3 cut(s) 590, 1177, 1327
DdeI CTNAG 1 cut(s) 581
DpnI GATC 2 cut(s) 5, 1186
DpnII GATC 2 cut(s) 3, 1184
EaeI YGGCCR 4 cut(s) 104, 289, 382, 456
EagI CGGCCG 3 cut(s) 104, 289, 382
Eam1104I CTCTTC 2 cut(s) 515, 662
EarI CTCTTC 2 cut(s) 515, 662
EciI GGCGGA 2 cut(s) 397, 461
Ecl136II GAGCTC 1 cut(s) 127
EclXI CGGCCG 3 cut(s) 104, 289, 382
Eco130I CCWWGG 2 cut(s) 459, 476
Eco147I AGGCCT 2 cut(s) 909, 1313
Eco24I GRGCYC 2 cut(s) 129, 486
Eco47I GGWCC 5 cut(s) 490, 544, 613, 1165, 1261
Eco52I CGGCCG 3 cut(s) 104, 289, 382
Eco53kI GAGCTC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 1307
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 2 cut(s) 482, 1261
EcoRI GAATTC 2 cut(s) 39, 607
EcoRII CCWGG 1 cut(s) 904
EcoT14I CCWWGG 2 cut(s) 459, 476
EcoT22I ATGCAT 2 cut(s) 717, 835
EcoT38I GRGCYC 2 cut(s) 129, 486
ErhI CCWWGG 2 cut(s) 459, 476
FaeI CATG 5 cut(s) 719, 812, 833, 1123, 1385
FaqI GGGAC 2 cut(s) 970, 1247
FatI CATG 5 cut(s) 715, 808, 829, 1119, 1381
FauI CCCGC 2 cut(s) 309, 995
FauNDI CATATG 1 cut(s) 835
FokI GGATG 3 cut(s) 1026, 1175, 1213
FriOI GRGCYC 2 cut(s) 129, 486
FspBI CTAG 3 cut(s) 477, 1148, 1172
GlaI GCGC 2 cut(s) 321, 396
GsaI CCCAGC 1 cut(s) 1142
GsuI CTGGAG 1 cut(s) 300
HaeII RGCGCY 2 cut(s) 323, 398
HaeIII GGCC 7 cut(s) 106, 291, 384, 458, 484, 909, 1313
HapII CCGG 7 cut(s) 103, 164, 437, 493, 539, 548, 616
HgaI GACGC 1 cut(s) 156
HhaI GCGC 2 cut(s) 322, 397
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 5 cut(s) 719, 812, 833, 1123, 1385
Hin6I GCGC 2 cut(s) 320, 395
HinP1I GCGC 2 cut(s) 320, 395
HincII GTYRAC 2 cut(s) 273, 688
HindII GTYRAC 2 cut(s) 273, 688
HinfI GANTC 6 cut(s) 328, 673, 983, 1213, 1221, 1441
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 7 cut(s) 103, 164, 437, 493, 539, 548, 616
HphI GGTGA 2 cut(s) 659, 1005
Hpy166II GTNNAC 6 cut(s) 273, 544, 688, 787, 1177, 1179
Hpy188I TCNGA 4 cut(s) 333, 723, 1165, 1220
Hpy188III TCNNGA 4 cut(s) 279, 848, 881, 1133
Hpy8I GTNNAC 6 cut(s) 273, 544, 688, 787, 1177, 1179
Hpy99I CGWCG 9 cut(s) 232, 256, 259, 262, 265, 403, 406, 418, 535
HpyAV CCTTC 8 cut(s) 58, 361, 374, 852, 1108, 1276, 1397, 1414
HpyCH4III ACNGT 6 cut(s) 734, 915, 998, 1297, 1326, 1365
HpyCH4V TGCA 6 cut(s) 715, 755, 833, 944, 1103, 1237
HpyF3I CTNAG 1 cut(s) 581
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 5 cut(s) 719, 812, 833, 1123, 1385
HspAI GCGC 2 cut(s) 320, 395
KflI GGGWCCC 1 cut(s) 1261
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 2 cut(s) 3, 1184
LguI GCTCTTC 1 cut(s) 515
LmnI GCTCC 7 cut(s) 67, 124, 132, 281, 366, 375, 1066
LweI GCATC 3 cut(s) 327, 1048, 1434
MaeI CTAG 3 cut(s) 477, 1148, 1172
MaeIII GTNAC 4 cut(s) 859, 1133, 1297, 1377
MalI GATC 2 cut(s) 5, 1186
MbiI CCGCTC 1 cut(s) 288
MboI GATC 2 cut(s) 3, 1184
MboII GAAGA 6 cut(s) 73, 220, 346, 532, 649, 750
MflI RGATCY 1 cut(s) 1184
MhlI GDGCHC 3 cut(s) 129, 486, 1008
MluCI AATT 7 cut(s) 39, 131, 607, 872, 977, 1056, 1253
MlyI GAGTC 3 cut(s) 337, 667, 992
MmeI TCCRAC 1 cut(s) 650
Mph1103I ATGCAT 2 cut(s) 717, 835
MreI CGCCGGCG 1 cut(s) 163
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 4 cut(s) 345, 687, 780, 957
MslI CAYNNNNRTG 2 cut(s) 710, 834
MspA1I CMGCKG 2 cut(s) 1142, 1289
MspCI CTTAAG 1 cut(s) 956
MspI CCGG 7 cut(s) 103, 164, 437, 493, 539, 548, 616
MspR9I CCNGG 3 cut(s) 494, 548, 906
Mva1269I GAATGC 2 cut(s) 772, 931
MvaI CCWGG 1 cut(s) 906
MvnI CGCG 2 cut(s) 381, 1194
NaeI GCCGGC 1 cut(s) 165
NciI CCSGG 2 cut(s) 494, 548
NdeI CATATG 1 cut(s) 835
NdeII GATC 2 cut(s) 3, 1184
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 5 cut(s) 719, 812, 833, 1123, 1385
NlaIV GGNNCC 9 cut(s) 69, 483, 484, 498, 546, 1167, 1186, 1262, 1263
NmuCI GTSAC 1 cut(s) 1133
NotI GCGGCCGC 2 cut(s) 289, 382
NsiI ATGCAT 2 cut(s) 717, 835
NspI RCATGY 1 cut(s) 1123
PaeI GCATGC 1 cut(s) 1123
PceI AGGCCT 2 cut(s) 909, 1313
PciSI GCTCTTC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 5 cut(s) 257, 260, 410, 512, 632
PctI GAATGC 2 cut(s) 772, 931
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 3 cut(s) 1213, 1221, 1441
PflMI CCANNNNNTGG 1 cut(s) 1022
PleI GAGTC 3 cut(s) 336, 667, 991
PpsI GAGTC 3 cut(s) 336, 667, 991
PpuMI RGGWCCY 1 cut(s) 1261
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 1261
Psp6I CCWGG 1 cut(s) 904
PspFI CCCAGC 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 904
PspN4I GGNNCC 9 cut(s) 69, 483, 484, 498, 546, 1167, 1186, 1262, 1263
PspOMI GGGCCC 1 cut(s) 482
PspPI GGNCC 7 cut(s) 482, 483, 490, 544, 613, 1165, 1261
PspPPI RGGWCCY 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 1324
PsuI RGATCY 1 cut(s) 1184
PvuII CAGCTG 1 cut(s) 1289
RsaI GTAC 3 cut(s) 591, 1178, 1328
RsaNI GTAC 3 cut(s) 590, 1177, 1327
RseI CAYNNNNRTG 2 cut(s) 710, 834
Rsr2I CGGWCCG 1 cut(s) 613
RsrII CGGWCCG 1 cut(s) 613
SacI GAGCTC 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 4 cut(s) 345, 687, 780, 957
Sau3AI GATC 2 cut(s) 3, 1184
Sau96I GGNCC 7 cut(s) 482, 483, 490, 544, 613, 1165, 1261
SchI GAGTC 3 cut(s) 337, 667, 992
ScrFI CCNGG 3 cut(s) 494, 548, 906
SduI GDGCHC 3 cut(s) 129, 486, 1008
SfaNI GCATC 3 cut(s) 327, 1048, 1434
SgrAI CRCCGGYG 1 cut(s) 163
SinI GGWCC 5 cut(s) 490, 544, 613, 1165, 1261
SmiMI CAYNNNNRTG 2 cut(s) 710, 834
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SphI GCATGC 1 cut(s) 1123
Sse9I AATT 7 cut(s) 39, 131, 607, 872, 977, 1056, 1253
SseBI AGGCCT 2 cut(s) 909, 1313
SspI AATATT 1 cut(s) 1047
SspMI CTAG 3 cut(s) 477, 1148, 1172
SstI GAGCTC 1 cut(s) 129
StuI AGGCCT 2 cut(s) 909, 1313
StyD4I CCNGG 3 cut(s) 492, 546, 904
StyI CCWWGG 2 cut(s) 459, 476
TaaI ACNGT 6 cut(s) 734, 915, 998, 1297, 1326, 1365
TaqI TCGA 2 cut(s) 849, 882
TaqII GACCGA 1 cut(s) 601
TasI AATT 7 cut(s) 39, 131, 607, 872, 977, 1056, 1253
TatI WGTACW 2 cut(s) 589, 1176
TfiI GAWTC 3 cut(s) 1213, 1221, 1441
Tru1I TTAA 4 cut(s) 345, 687, 780, 957
Tru9I TTAA 4 cut(s) 345, 687, 780, 957
TscAI CASTG 3 cut(s) 739, 1105, 1186
TseFI GTSAC 1 cut(s) 1133
Tsp45I GTSAC 1 cut(s) 1133
TspDTI ATGAA 3 cut(s) 818, 884, 1347
TspGWI ACGGA 4 cut(s) 245, 504, 673, 804
TspRI CASTG 3 cut(s) 739, 1105, 1186
Van91I CCANNNNNTGG 1 cut(s) 1022
Vha464I CTTAAG 1 cut(s) 956
VpaK11BI GGWCC 5 cut(s) 490, 544, 613, 1165, 1261
XapI RAATTY 3 cut(s) 39, 607, 977
XceI RCATGY 1 cut(s) 1123
XmaJI CCTAGG 1 cut(s) 476
XspI CTAG 3 cut(s) 477, 1148, 1172
Zsp2I ATGCAT 2 cut(s) 717, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.