MD06G1233800.v1.1

Monoglyceride lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010185
Physical Location & Seq
Forward (+)
36502108 .. 36507270
5163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1233800.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGGTCTGTTTGGGAAGGGCAGCGATTATAGAGAGTAGAATTCTGGTTTTTCACGCGGAGAAGAAGCAGAAGCCAAACAACAACCTCAACAGGAAACAACCAATCCGGCCGTTGGTCAAATCGGAGGAGCTCCAGTTCCGGTTCAAGTCATCATCTCCACCTCCGCCGGCGTCATCGGCATTGTTCTTTCAAGCCACAAGACCACCGCCGAAGAAGCAGCAGAAAATGGCGACGGATCGAGGCAGCGGCTTGTCATCGTCGTCGACGTTGATGTTGACTTCTGGAGCGAGCGGCCGCATAAACGCCCTCTTCTCGCTGCAGGTGCTGCGGAGTCTGATGATGCTGATTAACGCCTTCTTCCTGCTCCTCCTGCTCCCATTTCGCGGCCGCAAGCGAGCGCCTTTGTCGTCCGCTTCTTCGTCTGCGGCGGCGGCGGCGGTGGCGGCTTCGCCGGTCTTGGCGGAGAAGTCGGCCAAGGACGAGAGGCAGCCGAGGGTGCCGATGGTGCGGGTGCCGACAAAGATAGTGCCGTGGAAGTGCAGCTCGTCATCGCCGGTGGACCAGGAGGTGGCGGCGAGGAGGGCGTTAGCGAAGCTAAGAGTTGTACAAGATGATGATGAGAATTCGGTCAGGCAATATTCGCTCTTTGCGACTGCGAGAGGTGAAACTCTCTTCACGCAGAGTTGGACTCCCGTTTCCGTTAACATCAGGGGCGTAGTTATTCTTATGCATGGTCTGAACGAACACAGTGGAAGATACAGCGACTTTGCAAAGCAACTGAATGCTAATGGATTTAAGGTTTACGGAATGGATTGGATTGGTCATGGCGGGAGTGATGGGCTTCATGCATATGTACCTTCTCTCGATTATGCTGTTACTGATATGAAATTATTTCTCGAAAAGGTTTTAGCTGAAAATCATGGCCTACCGTGTTTCTGTTTTGGGCATTCCACAGGTGCAGCCATTGTCCTTAAGGTAATGCTTGACCCAAAATTTGAGTCCCGCATAGACGGTGTAGTGCTCACATCACCTGCAGTTGGGGTTCAGCCTTCGCATCCAATATTAGTGGTAATTGCTCCCATTGTCTCATTTTTGCTGCCAAGATACCAACTCAGTGCAGCAAATAAACAGGGCATGCCGGTTTCTCGTGATCCAGAGGCTTTAGTTGCCAAGTATTCGGACCCTCTAGTGTACACTGGATCCATCCGCGTAAGGACTGGATATGAGATTCTCCGAATCACATCCTACTTGCAGCAGAACCTAACCAAATTGATGGTACCCTTCTTCGTTCTCCACGGAACAGCAGACACAGTTACTGACCCCGAGGCCTCCCAGAAACTGTACGATGAAGCCTCCTCAACTGACAAAACCATCAAACTGTTTGACGGGTTCGTACATGACCTTCTCTTTGAGCCAGAGCGAGAAGCCATTACGAAGGATATCATTGACTGGTTGAATCATAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

490

Amino Acids

53.87

Weight (kDa)

9.58

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 236 - 473 2.1e-76 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 239 - 351 3.5e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 240 - 449 6.5e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 310, 1039
Acc36I ACCTGC 2 cut(s) 310, 1039
Acc65I GGTACC 1 cut(s) 1276
AccB1I GGYRCC 3 cut(s) 496, 511, 1276
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 1 cut(s) 263
AccII CGCG 3 cut(s) 56, 384, 1209
AclWI GGATC 4 cut(s) 243, 1145, 1194, 1207
AcoI YGGCCR 4 cut(s) 107, 292, 385, 471
AcsI RAATTY 3 cut(s) 39, 622, 992
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 6 cut(s) 606, 855, 1193, 1278, 1343, 1395
AfiI CCNNNNNNNGG 4 cut(s) 112, 383, 568, 1037
AflII CTTAAG 1 cut(s) 971
AgsI TTSAA 3 cut(s) 145, 191, 1456
AjnI CCWGG 1 cut(s) 561
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 4 cut(s) 130, 543, 595, 911
AluI AGCT 4 cut(s) 130, 543, 595, 911
Alw21I GWGCWC 2 cut(s) 132, 1023
Alw26I GTCTC 1 cut(s) 1090
AlwI GGATC 4 cut(s) 243, 1145, 1194, 1207
AlwNI CAGNNNCTG 3 cut(s) 325, 1316, 1339
Ama87I CYCGRG 1 cut(s) 1322
AoxI GGCC 6 cut(s) 107, 292, 385, 471, 922, 1326
ApoI RAATTY 3 cut(s) 39, 622, 992
ArsI GACNNNNNNTTYG 6 cut(s) 977, 983, 1009, 1015, 1391, 1423
Asp718I GGTACC 1 cut(s) 1276
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 2 cut(s) 559, 1180
AsuHPI GGTGA 2 cut(s) 674, 1020
AvaI CYCGRG 1 cut(s) 1322
AvaII GGWCC 2 cut(s) 559, 1180
BamHI GGATCC 1 cut(s) 1199
BanI GGYRCC 3 cut(s) 496, 511, 1276
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BauI CACGAG 1 cut(s) 1146
Bbv12I GWGCWC 2 cut(s) 132, 1023
BccI CCATC 5 cut(s) 496, 830, 1211, 1267, 1379
BceAI ACGGC 2 cut(s) 94, 514
BciT130I CCWGG 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 1090
BfaI CTAG 1 cut(s) 1187
BfmI CTRYAG 2 cut(s) 317, 1032
BfoI RGCGCY 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 971
BfuAI ACCTGC 2 cut(s) 310, 1039
Bme1390I CCNGG 1 cut(s) 563
Bme18I GGWCC 2 cut(s) 559, 1180
BmeT110I CYCGRG 1 cut(s) 1322
BmgT120I GGNCC 2 cut(s) 559, 1180
BmiI GGNNCC 5 cut(s) 498, 513, 1182, 1201, 1278
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 330, 1063
BplI GAGNNNNNCTC 2 cut(s) 673, 705
BpmI CTGGAG 2 cut(s) 116, 303
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 5 cut(s) 474, 491, 530, 1294, 1323
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 383, 568, 1037
Bse118I RCCGGY 4 cut(s) 166, 451, 553, 1138
Bse1I ACTGG 4 cut(s) 133, 1201, 1222, 1454
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 5 cut(s) 474, 491, 530, 1294, 1323
BseGI GGATG 3 cut(s) 1054, 1203, 1241
BseLI CCNNNNNNNGG 4 cut(s) 112, 383, 568, 1037
BseMII CTCAG 1 cut(s) 1126
BseNI ACTGG 4 cut(s) 133, 1201, 1222, 1454
BseRI GAGGAG 4 cut(s) 140, 356, 592, 1345
BseX3I CGGCCG 3 cut(s) 107, 292, 385
BsgI GTGCAG 3 cut(s) 559, 978, 1137
Bsh1236I CGCG 3 cut(s) 56, 384, 1209
Bsh1285I CGRYCG 3 cut(s) 110, 295, 388
BshFI GGCC 6 cut(s) 109, 294, 387, 473, 924, 1328
BshNI GGYRCC 3 cut(s) 496, 511, 1276
BsiEI CGRYCG 3 cut(s) 110, 295, 388
BsiHKAI GWGCWC 2 cut(s) 132, 1023
BsiHKCI CYCGRG 1 cut(s) 1322
BsiSI CCGG 6 cut(s) 106, 139, 167, 452, 554, 1139
BslFI GGGAC 1 cut(s) 985
BslI CCNNNNNNNGG 4 cut(s) 112, 383, 568, 1037
BsmAI GTCTC 1 cut(s) 1090
BsmFI GGGAC 1 cut(s) 985
BsmI GAATGC 2 cut(s) 787, 946
BsnI GGCC 6 cut(s) 109, 294, 387, 473, 924, 1328
BsoBI CYCGRG 1 cut(s) 1322
Bsp1286I GDGCHC 2 cut(s) 132, 1023
Bsp1407I TGTACA 2 cut(s) 604, 1191
Bsp143I GATC 3 cut(s) 235, 1150, 1199
BspANI GGCC 6 cut(s) 109, 294, 387, 473, 924, 1328
BspCNI CTCAG 1 cut(s) 1125
BspFNI CGCG 3 cut(s) 56, 384, 1209
BspLI GGNNCC 5 cut(s) 498, 513, 1182, 1201, 1278
BspMAI CTGCAG 2 cut(s) 321, 1036
BspMI ACCTGC 2 cut(s) 310, 1039
BspPI GGATC 4 cut(s) 243, 1145, 1194, 1207
BspT107I GGYRCC 3 cut(s) 496, 511, 1276
BspTI CTTAAG 1 cut(s) 971
BsrBI CCGCTC 1 cut(s) 291
BsrFI RCCGGY 4 cut(s) 166, 451, 553, 1138
BsrGI TGTACA 2 cut(s) 604, 1191
BsrI ACTGG 4 cut(s) 133, 1201, 1222, 1454
BssAI RCCGGY 4 cut(s) 166, 451, 553, 1138
BssECI CCNNGG 5 cut(s) 474, 491, 530, 1294, 1323
BssMI GATC 3 cut(s) 235, 1150, 1199
BssNI GRCGYC 1 cut(s) 170
BssSI CACGAG 1 cut(s) 1146
BssT1I CCWWGG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 1146
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 6 cut(s) 749, 930, 1013, 1312, 1341, 1380
Bst6I CTCTTC 2 cut(s) 314, 677
BstACI GRCGYC 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 2 cut(s) 604, 1191
BstC8I GCNNGC 5 cut(s) 168, 289, 392, 396, 1136
BstDEI CTNAG 2 cut(s) 596, 1112
BstDSI CCRYGG 2 cut(s) 530, 1294
BstF5I GGATG 3 cut(s) 1054, 1203, 1241
BstFNI CGCG 3 cut(s) 56, 384, 1209
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 3 cut(s) 238, 1153, 1202
BstMAI GTCTC 1 cut(s) 1090
BstMBI GATC 3 cut(s) 235, 1150, 1199
BstMCI CGRYCG 3 cut(s) 110, 295, 388
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 1138
BstSCI CCNGG 1 cut(s) 561
BstSFI CTRYAG 2 cut(s) 317, 1032
BstUI CGCG 3 cut(s) 56, 384, 1209
BstX2I RGATCY 1 cut(s) 1199
BstXI CCANNNNNNTGG 1 cut(s) 1273
BstYI RGATCY 1 cut(s) 1199
BstZI CGGCCG 3 cut(s) 107, 292, 385
BsuRI GGCC 6 cut(s) 109, 294, 387, 473, 924, 1328
BtgI CCRYGG 2 cut(s) 530, 1294
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 3 cut(s) 1054, 1203, 1241
BtsIMutI CAGTG 3 cut(s) 754, 1120, 1194
BveI ACCTGC 2 cut(s) 310, 1039
Cac8I GCNNGC 5 cut(s) 168, 289, 392, 396, 1136
CaiI CAGNNNCTG 3 cut(s) 325, 1316, 1339
CciNI GCGGCCGC 2 cut(s) 292, 385
CfoI GCGC 1 cut(s) 400
Cfr10I RCCGGY 4 cut(s) 166, 451, 553, 1138
Cfr13I GGNCC 2 cut(s) 559, 1180
CseI GACGC 1 cut(s) 159
Csp6I GTAC 6 cut(s) 605, 854, 1192, 1277, 1342, 1394
CspCI CAANNNNNGTGG 2 cut(s) 1047, 1082
CviAII CATG 6 cut(s) 731, 824, 845, 920, 1135, 1397
CviQI GTAC 6 cut(s) 605, 854, 1192, 1277, 1342, 1394
DdeI CTNAG 2 cut(s) 596, 1112
DpnI GATC 3 cut(s) 237, 1152, 1201
DpnII GATC 3 cut(s) 235, 1150, 1199
EaeI YGGCCR 4 cut(s) 107, 292, 385, 471
EagI CGGCCG 3 cut(s) 107, 292, 385
Eam1104I CTCTTC 2 cut(s) 314, 677
EarI CTCTTC 2 cut(s) 314, 677
EciI GGCGGA 2 cut(s) 153, 476
Ecl136II GAGCTC 1 cut(s) 130
EclXI CGGCCG 3 cut(s) 107, 292, 385
Eco130I CCWWGG 1 cut(s) 474
Eco147I AGGCCT 1 cut(s) 1328
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 1441
Eco47I GGWCC 2 cut(s) 559, 1180
Eco52I CGGCCG 3 cut(s) 107, 292, 385
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 1322
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 2 cut(s) 39, 622
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 1441
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 2 cut(s) 732, 850
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 6 cut(s) 734, 827, 848, 923, 1138, 1400
FaqI GGGAC 1 cut(s) 985
FatI CATG 6 cut(s) 730, 823, 844, 919, 1134, 1396
FauI CCCGC 3 cut(s) 501, 821, 1010
FauNDI CATATG 1 cut(s) 850
FblI GTMKAC 1 cut(s) 263
FokI GGATG 3 cut(s) 1041, 1190, 1228
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 1 cut(s) 1187
GlaI GCGC 1 cut(s) 399
GsuI CTGGAG 2 cut(s) 116, 303
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 6 cut(s) 109, 294, 387, 473, 924, 1328
HapII CCGG 6 cut(s) 106, 139, 167, 452, 554, 1139
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 6 cut(s) 734, 827, 848, 923, 1138, 1400
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 3 cut(s) 264, 276, 703
HindII GTYRAC 3 cut(s) 264, 276, 703
HinfI GANTC 6 cut(s) 331, 688, 998, 1228, 1236, 1456
HpaI GTTAAC 1 cut(s) 703
HpaII CCGG 6 cut(s) 106, 139, 167, 452, 554, 1139
HphI GGTGA 2 cut(s) 674, 1020
Hpy166II GTNNAC 7 cut(s) 264, 276, 559, 703, 802, 1192, 1194
Hpy188I TCNGA 5 cut(s) 124, 336, 738, 1180, 1235
Hpy188III TCNNGA 5 cut(s) 282, 863, 896, 1148, 1154
Hpy8I GTNNAC 7 cut(s) 264, 276, 559, 703, 802, 1192, 1194
Hpy99I CGWCG 4 cut(s) 235, 262, 265, 268
HpyAV CCTTC 7 cut(s) 9, 364, 867, 1059, 1291, 1412, 1429
HpyCH4III ACNGT 6 cut(s) 749, 930, 1013, 1312, 1341, 1380
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 9 cut(s) 319, 540, 730, 770, 848, 959, 1034, 1118, 1252
HpyF3I CTNAG 2 cut(s) 596, 1112
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 6 cut(s) 734, 827, 848, 923, 1138, 1400
HspAI GCGC 1 cut(s) 398
KpnI GGTACC 1 cut(s) 1280
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
KspAI GTTAAC 1 cut(s) 703
Kzo9I GATC 3 cut(s) 235, 1150, 1199
LmnI GCTCC 6 cut(s) 127, 135, 284, 369, 378, 1081
LweI GCATC 2 cut(s) 330, 1063
MaeI CTAG 1 cut(s) 1187
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 2 cut(s) 874, 1312
MalI GATC 3 cut(s) 237, 1152, 1201
MbiI CCGCTC 1 cut(s) 291
MboI GATC 3 cut(s) 235, 1150, 1199
MboII GAAGA 8 cut(s) 73, 223, 301, 349, 408, 664, 765, 1276
MflI RGATCY 1 cut(s) 1199
MhlI GDGCHC 2 cut(s) 132, 1023
MluCI AATT 6 cut(s) 39, 622, 887, 992, 1071, 1268
MlyI GAGTC 3 cut(s) 340, 682, 1007
MmeI TCCRAC 1 cut(s) 665
Mph1103I ATGCAT 2 cut(s) 732, 850
MreI CGCCGGCG 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 166
MseI TTAA 4 cut(s) 348, 702, 795, 972
MslI CAYNNNNRTG 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 246
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 6 cut(s) 106, 139, 167, 452, 554, 1139
MspR9I CCNGG 1 cut(s) 563
Mva1269I GAATGC 2 cut(s) 787, 946
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 3 cut(s) 56, 384, 1209
NaeI GCCGGC 1 cut(s) 168
NdeI CATATG 1 cut(s) 850
NdeII GATC 3 cut(s) 235, 1150, 1199
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 6 cut(s) 734, 827, 848, 923, 1138, 1400
NlaIV GGNNCC 5 cut(s) 498, 513, 1182, 1201, 1278
NmeAIII GCCGAG 1 cut(s) 516
NotI GCGGCCGC 2 cut(s) 292, 385
NsiI ATGCAT 2 cut(s) 732, 850
NspI RCATGY 1 cut(s) 1138
PaeI GCATGC 1 cut(s) 1138
PaqCI CACCTGC 2 cut(s) 310, 1039
PceI AGGCCT 1 cut(s) 1328
PcsI WCGNNNNNNNCGW 2 cut(s) 263, 647
PctI GAATGC 2 cut(s) 787, 946
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 1228, 1236, 1456
PflMI CCANNNNNTGG 1 cut(s) 568
PleI GAGTC 3 cut(s) 339, 682, 1006
PpsI GAGTC 3 cut(s) 339, 682, 1006
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 5 cut(s) 498, 513, 1182, 1201, 1278
PspPI GGNCC 2 cut(s) 559, 1180
PstI CTGCAG 2 cut(s) 321, 1036
PstNI CAGNNNCTG 3 cut(s) 325, 1316, 1339
PsuI RGATCY 1 cut(s) 1199
RsaI GTAC 6 cut(s) 606, 855, 1193, 1278, 1343, 1395
RsaNI GTAC 6 cut(s) 605, 854, 1192, 1277, 1342, 1394
RseI CAYNNNNRTG 1 cut(s) 849
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 4 cut(s) 348, 702, 795, 972
Sau3AI GATC 3 cut(s) 235, 1150, 1199
Sau96I GGNCC 2 cut(s) 559, 1180
SchI GAGTC 3 cut(s) 340, 682, 1007
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 2 cut(s) 132, 1023
SfaNI GCATC 2 cut(s) 330, 1063
SfcI CTRYAG 2 cut(s) 317, 1032
SgrAI CRCCGGYG 2 cut(s) 166, 553
SgrDI CGTCGACG 1 cut(s) 262
SinI GGWCC 2 cut(s) 559, 1180
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
SphI GCATGC 1 cut(s) 1138
Sse9I AATT 6 cut(s) 39, 622, 887, 992, 1071, 1268
SseBI AGGCCT 1 cut(s) 1328
SspI AATATT 2 cut(s) 638, 1062
SspMI CTAG 1 cut(s) 1187
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 1 cut(s) 561
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 6 cut(s) 749, 930, 1013, 1312, 1341, 1380
TaiI ACGT 1 cut(s) 269
TaqI TCGA 4 cut(s) 238, 263, 864, 897
TaqII GACCGA 1 cut(s) 616
TasI AATT 6 cut(s) 39, 622, 887, 992, 1071, 1268
TatI WGTACW 2 cut(s) 604, 1191
TfiI GAWTC 3 cut(s) 1228, 1236, 1456
Tru1I TTAA 4 cut(s) 348, 702, 795, 972
Tru9I TTAA 4 cut(s) 348, 702, 795, 972
TscAI CASTG 3 cut(s) 754, 1120, 1201
TspDTI ATGAA 3 cut(s) 833, 899, 1362
TspGWI ACGGA 4 cut(s) 248, 688, 819, 1311
TspRI CASTG 3 cut(s) 754, 1120, 1201
Van91I CCANNNNNTGG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 971
VpaK11BI GGWCC 2 cut(s) 559, 1180
XapI RAATTY 3 cut(s) 39, 622, 992
XceI RCATGY 1 cut(s) 1138
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 1 cut(s) 1187
Zsp2I ATGCAT 2 cut(s) 732, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.